LOCUS       DAVBCR010000001        68749 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCR010000001 DAVBCR010000000
VERSION     DAVBCR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.001"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(134..1882)
                     /locus_tag="VQ508_000001"
     CDS             complement(134..1882)
                     /locus_tag="VQ508_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489071.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1884..3608)
                     /locus_tag="VQ508_000002"
     CDS             complement(1884..3608)
                     /locus_tag="VQ508_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489072.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQ508_000003"
     CDS             complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQ508_000003"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2489073.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(4899..5768)
                     /locus_tag="VQ508_000004"
     CDS             complement(4899..5768)
                     /locus_tag="VQ508_000004"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2489074.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            complement(5770..7056)
                     /locus_tag="VQ508_000005"
     CDS             complement(5770..7056)
                     /locus_tag="VQ508_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2489075.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQ508_000006"
     CDS             complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQ508_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2489076.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(8144..9178)
                     /locus_tag="VQ508_000007"
     CDS             complement(8144..9178)
                     /locus_tag="VQ508_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2489077.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(9207..10640)
                     /locus_tag="VQ508_000008"
     CDS             complement(9207..10640)
                     /locus_tag="VQ508_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2489078.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            complement(10656..11642)
                     /locus_tag="VQ508_000009"
     CDS             complement(10656..11642)
                     /locus_tag="VQ508_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2489079.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(11644..12747)
                     /locus_tag="VQ508_000010"
     CDS             complement(11644..12747)
                     /locus_tag="VQ508_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2489080.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(12737..13582)
                     /locus_tag="VQ508_000011"
     CDS             complement(12737..13582)
                     /locus_tag="VQ508_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2489081.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQ508_000012"
     CDS             complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQ508_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2489082.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQ508_000013"
     CDS             complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQ508_000013"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2489083.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(15977..16453)
                     /locus_tag="VQ508_000014"
     CDS             complement(15977..16453)
                     /locus_tag="VQ508_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2489084.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQVWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQ508_000015"
     CDS             complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQ508_000015"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2489085.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(17821..18159)
                     /locus_tag="VQ508_000016"
     CDS             complement(17821..18159)
                     /locus_tag="VQ508_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2489086.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(18170..19273)
                     /locus_tag="VQ508_000017"
     CDS             complement(18170..19273)
                     /locus_tag="VQ508_000017"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2489087.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQ508_000018"
     CDS             complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQ508_000018"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2489088.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(20459..21526)
                     /locus_tag="VQ508_000019"
     CDS             complement(20459..21526)
                     /locus_tag="VQ508_000019"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2489089.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVENILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(21545..22399)
                     /locus_tag="VQ508_000020"
     CDS             complement(21545..22399)
                     /locus_tag="VQ508_000020"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2489090.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(22389..23066)
                     /locus_tag="VQ508_000021"
     CDS             complement(22389..23066)
                     /locus_tag="VQ508_000021"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2489091.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(23063..24226)
                     /locus_tag="VQ508_000022"
     CDS             complement(23063..24226)
                     /locus_tag="VQ508_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2489092.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(24911..26545)
                     /locus_tag="VQ508_000023"
                     /pseudo
     CDS             complement(24911..26545)
                     /locus_tag="VQ508_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26526..27275)
                     /locus_tag="VQ508_000024"
     CDS             complement(26526..27275)
                     /locus_tag="VQ508_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489093.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            27454..28908
                     /locus_tag="VQ508_000025"
     CDS             27454..28908
                     /locus_tag="VQ508_000025"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2489094.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQ508_000026"
     CDS             complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQ508_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2489095.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31638..32123)
                     /locus_tag="VQ508_000027"
     CDS             complement(31638..32123)
                     /locus_tag="VQ508_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2489096.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(32398..32637)
                     /locus_tag="VQ508_000028"
     CDS             complement(32398..32637)
                     /locus_tag="VQ508_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489097.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(32890..33960)
                     /locus_tag="VQ508_000029"
     CDS             complement(32890..33960)
                     /locus_tag="VQ508_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2489098.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(33957..34730)
                     /locus_tag="VQ508_000030"
     CDS             complement(33957..34730)
                     /locus_tag="VQ508_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489099.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(34727..35533)
                     /locus_tag="VQ508_000031"
     CDS             complement(34727..35533)
                     /locus_tag="VQ508_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489100.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(35514..36671)
                     /locus_tag="VQ508_000032"
     CDS             complement(35514..36671)
                     /locus_tag="VQ508_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489101.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQ508_000033"
     CDS             complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQ508_000033"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2489102.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(37792..38544)
                     /locus_tag="VQ508_000034"
     CDS             complement(37792..38544)
                     /locus_tag="VQ508_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2489103.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQ508_000035"
     CDS             complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQ508_000035"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2489104.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            39427..40698
                     /locus_tag="VQ508_000036"
     CDS             39427..40698
                     /locus_tag="VQ508_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2489105.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(40718..41533)
                     /locus_tag="VQ508_000037"
     CDS             complement(40718..41533)
                     /locus_tag="VQ508_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2489106.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQ508_000038"
     CDS             complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQ508_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2489107.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQ508_000039"
     CDS             complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQ508_000039"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2489108.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQ508_000040"
     CDS             complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQ508_000040"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2489109.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQ508_000041"
     CDS             complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQ508_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2489110.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQ508_000042"
     CDS             complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQ508_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2489111.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQ508_000043"
     CDS             complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQ508_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2489112.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(47183..48487)
                     /locus_tag="VQ508_000044"
     CDS             complement(47183..48487)
                     /locus_tag="VQ508_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2489113.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(48488..49261)
                     /locus_tag="VQ508_000045"
     CDS             complement(48488..49261)
                     /locus_tag="VQ508_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2489114.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(49251..49529)
                     /locus_tag="VQ508_000046"
     CDS             complement(49251..49529)
                     /locus_tag="VQ508_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2489115.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(49529..49930)
                     /locus_tag="VQ508_000047"
     CDS             complement(49529..49930)
                     /locus_tag="VQ508_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2489116.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(50140..50757)
                     /locus_tag="VQ508_000048"
     CDS             complement(50140..50757)
                     /locus_tag="VQ508_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2489117.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(50747..51520)
                     /locus_tag="VQ508_000049"
     CDS             complement(50747..51520)
                     /locus_tag="VQ508_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2489118.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(51531..52268)
                     /locus_tag="VQ508_000050"
     CDS             complement(51531..52268)
                     /locus_tag="VQ508_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2489119.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(52273..53403)
                     /locus_tag="VQ508_000051"
     CDS             complement(52273..53403)
                     /locus_tag="VQ508_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2489120.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(53487..53876)
                     /locus_tag="VQ508_000052"
     CDS             complement(53487..53876)
                     /locus_tag="VQ508_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2489121.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(53892..54275)
                     /locus_tag="VQ508_000053"
     CDS             complement(53892..54275)
                     /locus_tag="VQ508_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2489122.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(54276..55064)
                     /locus_tag="VQ508_000054"
     CDS             complement(54276..55064)
                     /locus_tag="VQ508_000054"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2489123.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(55057..55992)
                     /locus_tag="VQ508_000055"
     CDS             complement(55057..55992)
                     /locus_tag="VQ508_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2489124.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(56122..56298)
                     /locus_tag="VQ508_000056"
     CDS             complement(56122..56298)
                     /locus_tag="VQ508_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2489125.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(56438..57145)
                     /locus_tag="VQ508_000057"
     CDS             complement(56438..57145)
                     /locus_tag="VQ508_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2489126.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            57296..58324
                     /gene="queA"
                     /locus_tag="VQ508_000058"
     CDS             57296..58324
                     /gene="queA"
                     /locus_tag="VQ508_000058"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2489127.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(58995..59384)
                     /locus_tag="VQ508_000059"
     CDS             complement(58995..59384)
                     /locus_tag="VQ508_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2489128.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(59438..60145)
                     /locus_tag="VQ508_000060"
     CDS             complement(59438..60145)
                     /locus_tag="VQ508_000060"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2489129.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(60845..61396)
                     /locus_tag="VQ508_000061"
     CDS             complement(60845..61396)
                     /locus_tag="VQ508_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489130.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQ508_000062"
     CDS             complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQ508_000062"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2489131.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(62615..64075)
                     /locus_tag="VQ508_000063"
     CDS             complement(62615..64075)
                     /locus_tag="VQ508_000063"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2489132.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(64191..64679)
                     /locus_tag="VQ508_000064"
     CDS             complement(64191..64679)
                     /locus_tag="VQ508_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489133.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(64669..64878)
                     /locus_tag="VQ508_000065"
     CDS             complement(64669..64878)
                     /locus_tag="VQ508_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489134.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(65268..65710)
                     /locus_tag="VQ508_000066"
                     /pseudo
     CDS             complement(65268..65710)
                     /locus_tag="VQ508_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66002..66808)
                     /locus_tag="VQ508_000067"
     CDS             complement(66002..66808)
                     /locus_tag="VQ508_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489135.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(67214..67942)
                     /locus_tag="VQ508_000068"
                     /pseudo
     CDS             complement(67214..67942)
                     /locus_tag="VQ508_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(68178..68489)
                     /locus_tag="VQ508_000069"
     CDS             complement(68178..68489)
                     /locus_tag="VQ508_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489136.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(68504..>68749)
                     /locus_tag="VQ508_000070"
     CDS             complement(68504..>68749)
                     /locus_tag="VQ508_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET2489137.1"
                     /translation="RNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMEL
                     LTIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT        20483 a        15452 c        12769 g        20045 t
ORIGIN      
        1 acctacggct agcttcctag tttgctcttt gattttcatt gagtattaca gtaaacaatt
       61 tttctagcta ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc
      121 aaaagagaat ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac
      181 cctttctatc catgagttct tgatggttac catattcaac aatatcacca tctactaaga
      241 caagaattaa atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc
      301 ggcccttcat gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg
      361 aagaagttgc ctcgtctaag ataagaatct ttggaatagc cagaaagact cgggatatgg
      421 tcaagagctg agcttggccg acagagagag attctccagc attttccaac ttggtatcgt
      481 atccctgtgg caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta
      541 cttgctctcg actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg
      601 tgagccaggt ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat
      661 cataaatgga ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa
      721 ggagattgat aagagttgat tttccagcac ctgtcggacc aacgatggct accttactac
      781 cagctggaat atcgatagac aactccttaa tcaaaatttt ttcaggatgg tagccaaaag
      841 agacatgttt aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg
      901 tttcagccac ttcagggcta tctaagaccc catagatacg ctctacgcaa gccagagcac
      961 tttgcaactc agctagcact gaagaaatat cgttaaaggg cttggtgtat tgctgaacat
     1021 agttcaaaaa agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag
     1081 ctcctactcc agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga
     1141 ccgttgaaga ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac
     1201 gcaatctttg gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa
     1261 gcgattcttc aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat
     1321 gataggatct cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga
     1381 ctaagagagt catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca
     1441 aaacaccaat gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatat
     1501 ccgtggttac acgactgacc atctctccat tgccttgccg atccgcaaaa gcaatcggta
     1561 accgatggag tttatggatg attcgctctc gtaaatctct ggtataagag aagattagac
     1621 gattatagag gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca
     1681 tctggagaaa aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga
     1741 cctgcccaat cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa
     1801 aggctaggaa aaggaggaag ggatggtttg ctaaatctac gactaaacgt ttgagcgtct
     1861 ggtttacagt ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca
     1921 cgatagactt gactggattt catcaagtca tcgtgcttgc caacagctag caactcacct
     1981 ttttccaaga ggagaatctg gtccgccatc tggagtgtcg aagtccgttg agaaatcaag
     2041 attaagctca tatttggcag attttcgcgg atagctttca agagcttgga ctctgtaatg
     2101 gtgtccagtg ctgaggtcgc atcatctagg atgagaaacg gagcctggcg caagactgct
     2161 cgtgcgatag acagcctttg tttttgtcca cctgagaaat ttcgccctcc tgcttcaact
     2221 agagcatcca agagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag
     2281 gcttgccaga gttcttgatc agtcacttct tgatttaaac ctaaagtcaa gttggaacga
     2341 atagttccct taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc
     2401 tccaaattaa gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga
     2461 taaagtccaa gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata
     2521 ccaaggattt gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca
     2581 tcaggatagg taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt
     2641 tgttctaatt ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg
     2701 gctgagatat aggactggtt gagggaattg atcagcatgg ctagcttgac caattccacc
     2761 aaaatctgta agaggtaatt gataagagca atgagagcac cttgactgag tactcctcct
     2821 tgaattgaaa tatagccttg ccagataata acgagaagag ttccattgac aatcagatag
     2881 gtcagaggtg ttaataaact agacaagaaa cctgtctttt cttgtaatct agcataaact
     2941 tggttaaggg tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc
     3001 cgcatccctt gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga
     3061 ctactgtaga gaggattgac caatcgagat aaccctacaa tgacaatggt caaaatggca
     3121 accatgacta agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca
     3181 ccaaaaacga taatgggcgc tcgtaaaaag agacgcagga attgattgat accagtctga
     3241 atctggtagg tatccgaagt caagcgagta accaaactag aagttgtcag acggtctctg
     3301 ctgtcctttg gcaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaaa
     3361 cctactgctg cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact
     3421 gcaaagataa ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa
     3481 gattggtcaa caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac
     3541 agcttgaaca agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg
     3601 tgtttcataa atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct
     3661 ctgttttatt tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc
     3721 ttggccacaa agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc
     3781 ggtccatctg tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt
     3841 cgctccattc catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg
     3901 ctaaaacttg gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca
     3961 cctgtcgtaa tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat
     4021 ggagcctctg tagcagcttc ttgtgtgaca cgataagact cttcagatag actggccttc
     4081 aacacctctt gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg
     4141 gtcacatcga tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct
     4201 tcagccagaa tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg
     4261 cgttcctgct cctgcaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa
     4321 atcccagttc gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg
     4381 ataactcgga aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc
     4441 acttggaccg tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact
     4501 tgaccattag cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc
     4561 tctaggcccc aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta
     4621 tcacgttttt aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc
     4681 gtaggtatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg
     4741 acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagcttc
     4801 ctagtttgct ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac
     4861 cctctggtca agagtttccc catcctatct tctattcttt attttgcttc tacacggaca
     4921 cctttggtat caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct
     4981 gccttaattg ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca
     5041 gaaaggtagg ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct
     5101 cttaccaagt cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg
     5161 gtcaccatgt ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca
     5221 acagcttcct tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat
     5281 tcatagtttg gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag
     5341 acttgccctt caacagaact tgcaataacg agattaccat aaatggctgg agccacattg
     5401 tcaggatgcc cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt
     5461 tgacccagtt ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa
     5521 ccgcgcgcca aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca
     5581 atttgcaaag cgattttaag caagagatta cgctcgtcat gtggaatcca tttgccaatc
     5641 tggtgttcaa tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt
     5701 acagctacac cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata
     5761 atcttcatct tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt
     5821 cttcaattca gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac
     5881 gacacgcgcc ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg
     5941 agcattgaag atttcagcca acttcaagac ctgacctttt gagtctagag ccaagattga
     6001 gaaatagtag tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc
     6061 gttgaagtct ttgccaatag taccatcatt caaacgacga acgatacgga caatatcagc
     6121 tacaacactt gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc
     6181 aataccgata gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg
     6241 cgctttaggt aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat
     6301 agaaccaacc aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt
     6361 gatattgcgg attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa
     6421 ttggctcaaa ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc
     6481 gctttctgca aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc
     6541 ttccaccatc ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt
     6601 aattttatca gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac
     6661 tgctgcttcg tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc
     6721 atggacagct aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg
     6781 agtgataaag gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc
     6841 ctggtctgat aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag
     6901 caagcgattt ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg
     6961 tgctgattga ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac
     7021 ggtaccaaat cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa
     7081 tatagccatt ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta
     7141 taaaaagaaa aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag
     7201 aaaagattag ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc
     7261 atactcttgt aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc
     7321 caacagccaa gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata
     7381 atagcgctcg ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc
     7441 aaaagctacc aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt
     7501 cttactctca gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc
     7561 aagagattga atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt
     7621 tttgataaat tcatctggag acatttgagc caattcttcc atatctggca aatccgataa
     7681 gtcttcaaaa tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc
     7741 aggttttgga gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc
     7801 tagctcatcc aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc
     7861 agcaatctcc attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt
     7921 atcactaatt tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat
     7981 actagatttt tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg
     8041 gtctttctat aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa
     8101 atcggctaag agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg
     8161 ctacaccgag gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct
     8221 tataacgagt cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg
     8281 atacatcctg taaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt
     8341 tatctaggat agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg
     8401 acaaaacatc tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact
     8461 gagaaaaatc acctactcta ttgattgttg gattaaagag ataaactaac acatttccca
     8521 tcacaaccaa aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca
     8581 catttaagcc aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca
     8641 gtttttcaat tttcacattt gcataggcat gtttaaattt ctcaatcaac ccatcaattt
     8701 ttttctctaa caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg
     8761 catcgtcaat catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt
     8821 tcaaggttgt ttctgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta
     8881 catcattgaa aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac
     8941 tataaccacc aatttgatga ttcaaaatcg ttttggccga ctcttttgga ggagtgattt
     9001 tatagataag ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg
     9061 ctttaattgt aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg
     9121 cttttcgctt tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga
     9181 tttttttctc ctttgtttag tagatactag ttttcctttg taagcatttt tgctataaat
     9241 ataatcacaa gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa
     9301 aagctgcttc ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg
     9361 attgtccttg agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa
     9421 ctaagcagaa agactccaat cataccatag tcggtataca actccatgat ataactactt
     9481 ccgataccat gccctttcag gtattccttg ttcaagacaa gataggatag attgtgggca
     9541 taactattac tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct
     9601 ccgaaaatgg ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa
     9661 ttacggaaat ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca
     9721 aaactagtcc cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgggaa
     9781 acttggacat tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa
     9841 cctacaaaaa tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc
     9901 cgcataaagt aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca
     9961 attgccaaat gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat
    10021 ttctttggct tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg
    10081 taggtaaaat aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc
    10141 aaacgataca agagccgttc aaataaccga atgaaataga aaggataagt tagaagaaaa
    10201 actcctagtg atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata
    10261 tttgctactt ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc
    10321 aagcccagaa tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag
    10381 gtatccaaag caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa
    10441 atggttaaat agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt
    10501 aagctactaa ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac
    10561 agatacacaa ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat
    10621 aatcgtttcc catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca
    10681 atatttttca gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta
    10741 attcttctcc ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa
    10801 aaacgatgaa tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta
    10861 tccagaataa agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga
    10921 tagtttttca caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc
    10981 tctgcatagg gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta
    11041 gtattaacca catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg
    11101 taaatcaacc ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg
    11161 caaatcgtcc caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca
    11221 cagacaaaat agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc
    11281 atgacaccac agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga
    11341 atcatttctg gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc
    11401 tgcttcatcc catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac
    11461 accctgtcat cttgttcagc gattgagtca cacaagcgac cactttcatc tgttgcacca
    11521 tcatcaacaa gaataatttc cagattttga tagatctgct tctgaatgga agctatcgat
    11581 ttttctaggt actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc
    11641 atgctactcc tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg
    11701 aatgaattgg ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat
    11761 cgcctgagct gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa
    11821 ttcctcagcc cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac
    11881 atacactcca ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagataa
    11941 atactgataa ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc
    12001 tttgactcgt tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata
    12061 gttttttccc tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt
    12121 ttcctcaatc cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc
    12181 ttgagatttt tctagaatag tctgaaaatc atatccattg tagattatct gtaatttaga
    12241 agcataatct ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat
    12301 tgtattcgca gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag
    12361 ttcttcaata cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa
    12421 cagtggtgga ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa
    12481 acgacgagtc agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct
    12541 ggtttggcga taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata
    12601 tcccttgtca aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt
    12661 tgaaacaatg gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa
    12721 aaataagatt tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca
    12781 tatcagcgat gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat
    12841 aatccccaaa agcagttctg agatagacat catagccgat tggaataggc atctctgttc
    12901 cttcaaatgg caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt
    12961 agccaggacc tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca
    13021 tttctttctc agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta
    13081 aaatgcgact tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa
    13141 agagtgaata aatcaaggcc caacgaacct gttttttccg ctcagctgga tttttcggat
    13201 aataatccaa aggcaaaaca tccaaggtca gaccatgtgg caaatctaaa tcctgctgat
    13261 aaggcttgat acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa
    13321 aatccttgtg actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg
    13381 ctaatttctc ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa
    13441 atcccttgtt tcgaagggca ccaatagcgc ctccgccaca gagataacag agcaaatcat
    13501 gttctttaca aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt
    13561 ttaaatcagt catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca
    13621 aggggtgaaa atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct
    13681 atcaaacttt gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt
    13741 caccttgccg taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt
    13801 gtttttagca gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc
    13861 ttatctcaag cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc
    13921 caagcctcca taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt
    13981 ttcaatacca ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata
    14041 gggtacgttg gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag
    14101 tttagtggga tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt
    14161 acggccgtaa tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc
    14221 attcatctgc tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcgta
    14281 taaatcatct ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc
    14341 atcaatcgtc accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc
    14401 aaccaaggcg tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat
    14461 ttctgcccct ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc
    14521 tgtctgtttt ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg
    14581 catgaaactg actagcttca ttttcttatc tgagcccccg acatttaata ccataataga
    14641 gtcagttcgt gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat
    14701 attaactcca tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc
    14761 agcagttttc tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa
    14821 agccacacag accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg
    14881 tcttttcttt tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt
    14941 cgcatagctt ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt
    15001 tcccctagca agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt
    15061 gagatagtga atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg
    15121 attttcttta ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca
    15181 cccagaattt tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag
    15241 tccttatcga tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt
    15301 ggacgacttt caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcctta
    15361 taaagtgcac ctggaaggtt gtcaggtaat gtcaaggcca aactcatctt ttcagtttgt
    15421 gcttgcaagg gaatactagg cttttcagct cctagaaccc agaaacgtgt gaaattggct
    15481 tccatttcct gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta
    15541 ggtgcaactg ctgcaaaggg ctggtctgga tgttcggaaa taaaacgggc cgcataagct
    15601 gtactagctg ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct
    15661 tgagccaagg cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc
    15721 atcaactgct gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga
    15781 aaaagatagt ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca
    15841 gaatagtcca ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc
    15901 tgcaattcct catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga
    15961 cctagataag ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca
    16021 catccaaaac ccgactagcc acttcctcat accaaacctg tctttcttga aaaatagcta
    16081 ctagttcttc cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga
    16141 tacgtaggta gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga
    16201 gtaagtcacg atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg
    16261 tttgtagtaa atcagctagg acttctgatt ctacatgacg aaaggctgct tctccctttt
    16321 cagcgaaaaa attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa
    16381 ggtaattagg gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata
    16441 acaccttagc catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg
    16501 atggcttctg cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct
    16561 gtcatcatgc cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat
    16621 ttccctttga taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag
    16681 gcgtctgcca caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg
    16741 ataactgtta caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc
    16801 aatcgtggaa tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc
    16861 aaggtagcag atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca
    16921 cgaatgacat caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaga
    16981 cgagaatttg gagcaatcaa acctgcgact aaccaaaagg ctgcactgga aatatctcct
    17041 ggtacgacca ccttctgtcc tgtcaatttt tgtggcccct ggactgtgat tttcttacca
    17101 tccacactta aatgaccacc aaattgttgc aacatatctt cagtatgatt acgggtgtac
    17161 tctttttcga taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac
    17221 ttgacttggg cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct
    17281 tttaagcgaa ggggaggcaa gtctcgttca gtttgccctg aaatgctgac gcccattttt
    17341 ttcagtggaa gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc
    17401 tctacttcga aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa
    17461 tttcccatat taagggcatt ttgtggcgct tttaagccag ccatgcctac accttgaatg
    17521 gtaataaccc catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc
    17581 gaaagaacgt cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt
    17641 ccaaagataa tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt
    17701 aaatggcgaa tgtttgtttt tagtttcata ctggacctca tacttgcaat acttttacct
    17761 attttatcat aaaaagtcag aaattcctta aaaattcctg actttaggat cgttcttttc
    17821 ttatttcagc aattctgaaa ctggttcaaa aacaattttt tcaatgtcag aaaggtaaat
    17881 ggccaattgt tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt
    17941 accaaagcct tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag
    18001 tttttgaatt tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc
    18061 tgctgcaatc gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc
    18121 gcgactgagt tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttgatga
    18181 cgactgtata gtcggtattt tctgttatga gatgctcagc tctttccaag tcttgagcat
    18241 ttttaaatga aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga
    18301 tgttaaccaa ggaagttcca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat
    18361 caggaacgtc aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca
    18421 tggtctggcg ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct
    18481 tactaatggc ctgaccaatc acttccaaac gttccttgaa atcctcaatt ctatccagaa
    18541 tggtctcgct attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc
    18601 gggtcatatc tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat
    18661 agaccgcagt ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag
    18721 aagtgacacg atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa
    18781 gcagatcctt catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat
    18841 aataggcatt ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag
    18901 ccatgggatg ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat
    18961 ccacaatggt tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca
    19021 aattggccaa ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga
    19081 catctgccaa aggagcaaaa ctagcaaaat catccgttgc acggtcaatc ataccttctt
    19141 tcaaggcgat atctctcgaa gcttgactac gattataacc taaaatttca taatctggat
    19201 gatcgcgttt gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga
    19261 ttgtttttgc cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg
    19321 cttcttttag ttcctcaaga ttatctgatg agaatttttc gaggatttct tgcgccagaa
    19381 ccgttgctac aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc
    19441 tctccacggt tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat
    19501 aaagagtagg aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac
    19561 caccttcaaa accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga
    19621 gaatttcatc cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt
    19681 ccaccccttt aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc
    19741 tatcccattg gacataggaa ccaagaccaa ctggaacgcc tccgacgact gtctccacaa
    19801 ccccaccgat ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt
    19861 ctcgttcttg gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga
    19921 ctgtcagatt ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa
    19981 tctccatatc cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg
    20041 tggtttcacg agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa
    20101 tccccccaac caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa
    20161 ggcggtcttc aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga
    20221 tgacatccat agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga
    20281 caacctggtc actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc
    20341 taagatcctc attgatatcc tcagctgtca atggaagtcc agctggaatt ccctcaataa
    20401 tagctgttag acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt
    20461 attttaccaa gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa
    20521 gctctggcac caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag
    20581 cctgataaag cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac
    20641 acatctctgt gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct
    20701 tggaaatctg taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg
    20761 cagtcgcttc gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat
    20821 tgtccaactc atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg
    20881 tctctgcatg ttccaaaata ttctcaacag aaccattcag tcccgtcaag agagcccaca
    20941 gttctggatc ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc
    21001 tttttccgag ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg
    21061 tccccaccat atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat
    21121 caacctgagc tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag
    21181 aggctacaaa gccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc
    21241 tacgagtcag accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct
    21301 ttctttcttc accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc
    21361 tgagcttgac cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct
    21421 tttgcggttg ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa
    21481 tctgaatatc ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct
    21541 ttctttattg atatttttct gttaaagact gccaaatctc ttctgtcggc attttcttgc
    21601 ctgtccacag ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg
    21661 ctggattgcc ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga
    21721 tatctgcaac taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc
    21781 catccatgcc cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca
    21841 gttcagaaac atattctaaa gcacacaaat ccactttaaa gcctgtctgc tcctgtaact
    21901 tgtctaggta aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac
    21961 tgacgccatc caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca
    22021 gggtcatctt tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat
    22081 ctgtattata tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca
    22141 agcgcgcttc atcgctcagc tcatccaaat aaggaatcac ctgctcctta tagggcatgg
    22201 acagattgat gccaaacatc tggtagcgac gaatattggc cactgtttct accaagtcac
    22261 tcgcttcaat ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat
    22321 tgtggatgaa gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg
    22381 tatagccatc aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc
    22441 tgcccagggg cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc
    22501 gcagtgatac gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca
    22561 ggattgagcg ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg
    22621 tgagccatca ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt
    22681 tccatcatgt tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag
    22741 tccagcattt cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt
    22801 tgatagagtt gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt
    22861 tctccacctt cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca
    22921 aaaatggctg gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca
    22981 atgatgtcgg catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt
    23041 ggcattactg aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat
    23101 tactactttc atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa
    23161 tctggtaact tcttcctgca aagcctttat caatttgttc tgtaaatttc tgacgggaaa
    23221 cattggcagc attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct
    23281 gggaaatcaa cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa
    23341 agctaggcgg atctaggaca atcacatcgt aggtcaagtt tttgcgtttg gcatatttga
    23401 aatactcaaa gacatccatg actataaaac gacgctcgtc tgtgctgagc ccatttgcct
    23461 gaaaatgcgc ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc
    23521 tagctcctcc catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca
    23581 aggatttacc catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga
    23641 aaattcctgt catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa
    23701 cagtgaaaaa gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct
    23761 taaagcggat tttctcataa gcccctaaaa cctcagggaa aacctgtcta aaggcttctg
    23821 atatagtctg acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc
    23881 cataaaggtc cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg
    23941 cagttgtcaa atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg
    24001 tttcaaagaa agcttgattg aaggccacct tgtctttgct gacaaaccag cccaagccct
    24061 tgttttgctg agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta
    24121 cttcctgatc cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct
    24181 tagcaagctt cttttcaacc cttttgctga ctcttattct attcataact accattatat
    24241 caaactttta gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa
    24301 aaatggtata ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacaataaaa
    24361 aatgctcttc cgtcttggag gagcatttct ttttatcaac gaaaatcaaa taacaaacta
    24421 tgaaactagc ctcaggttaa ctgtgagatt ataggtagag aggttgaatc agcaatatgt
    24481 gtctgtcaaa tttagtgaca aaggtagtag aagaaagatg aagaaataaa tctgcttcag
    24541 tagatatctg gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat
    24601 caataaataa tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat
    24661 ttcgaactct aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta
    24721 acgtttttga aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta
    24781 tattatattt cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt
    24841 tacaactaca ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca
    24901 accaactatc tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc
    24961 caaaaatagc aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga
    25021 taggggagag agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac
    25081 tataaataaa caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa
    25141 tgctactcca attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga
    25201 tgacactggc aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta
    25261 gatatagaga aagaccgagc aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa
    25321 tccaaaattt cactttcaca taacgagcaa agtcaaaggg taaactcttc agaaaatcaa
    25381 cattttccct ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa
    25441 ctgctataaa cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa
    25501 ctatctgaga atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag
    25561 cacttgtaaa aagcactgta atcacaccag tcccctatcc caagagggtg aggtggtagc
    25621 gtaaaaccat acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt
    25681 cttttttgac cttctcctca ctgttaagca ggatcacgtc ataaaaacga ggaaggacct
    25741 tctttttggt cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta
    25801 aggcttctgt cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga
    25861 gaaatggaat gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga
    25921 taaatattaa aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat
    25981 actttctgaa gaccctagtc tgagccaaga aatgtactgc cactaccgtc actaaagtaa
    26041 cagagacaaa taataaggtc aaggacagta gcatcaaagg caaacccagc caaagagaag
    26101 gagctagact aatatagagg gctagaaaat aagctaggat tggtacaatt ccagttagag
    26161 ctggcaagag gacagacaga cctttggcca ttataatctc tgattcttta aaggcatagg
    26221 gcctatagga ggccaggtcc ttactctcat aaaagacatt gtaaaaggca gtgaaagaag
    26281 ttgaaaaggc cagcaccagt aaaatagcaa ccatcgagct aaaataaata ggtatttcct
    26341 caaaaggaaa atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa
    26401 tgtaagaact taggactcta gcagaaacat ttactttttt cccaggattc ttagcctgct
    26461 tctttcgtag attagccaga ttagcttctt gagatgaata gaggatattg atatcaacta
    26521 attttttaat gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg
    26581 tagatacttt ccaaaaactg gtctgggtgg tctttcctca agttctctac actaccacaa
    26641 tagatcaaat gccccttttt caaaatagca atccgatcac agacttgctc ggctacctca
    26701 aggacatgag ttgaaaacaa gaccgtcttg cctttttgtg catgttcctt catcatttgc
    26761 ttcaaatcaa aggcggcctg gggatccaaa ccagtcaagg gttcgtccaa aacccaaata
    26821 tcaggagcag acaagagtgc cccaatgaca aagactttct gacgcattcc gtgagaaaga
    26881 gtttcaataa cctgatagcg gttttcagca aaatcaaaaa cgttcaatag cctagctaga
    26941 ctagtctcca agtcagagct agtcagatca taggatgaag cgatcaattc ccaaaattca
    27001 ttggccgtta agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgtttg
    27061 atcgccaagc gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt
    27121 gaaatgatac tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc
    27181 aagcccataa tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca
    27241 ccatacaact tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta
    27301 ttatacatcg ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt
    27361 aaaatagttt ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat
    27421 aaagaacaag attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac
    27481 tttgaatggt atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca
    27541 cacctagctc atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac
    27601 gaaaaagatg tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa
    27661 gggactgtcc gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt
    27721 aaagaacagg gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat
    27781 aacagggaag cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga
    27841 gaacccttca ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat
    27901 aatggcttcc actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt
    27961 aaatctggga tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc
    28021 gataacgaaa acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct
    28081 gaagtcatcc aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct
    28141 ggtttccgtt tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc
    28201 gatatgaagg aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac
    28261 aaggaagcca atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt
    28321 cgtctccacc agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc
    28381 attttcacag atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac
    28441 cacgataccc aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca
    28501 gcctatgccc tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac
    28561 tatgggattt caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta
    28621 gccatccgaa aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt
    28681 atcggttggg tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat
    28741 gaccaagaaa acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta
    28801 gatttacttg gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc
    28861 cctgtctcag ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata
    28921 accaagctag gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg
    28981 gatagatctt tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta
    29041 gcttggttgc ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg
    29101 attggtgcta attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca
    29161 aggacattga ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt
    29221 tgtttccagt tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta
    29281 gcaatgtaac gatgaccgtt gacttcctta gcatctttga agatatcgcc tgcggctgca
    29341 gctgcggctt tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga
    29401 agtccttcta ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca
    29461 gctttaagag catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc
    29521 aagatacggc gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc
    29581 tcagaagtgt tgccaacatg agtaccacca caaagttcaa tagagtagtc accgatagtc
    29641 acgacacgaa cttccttgcc gtatttctca ccaaagaggg ccatagctcc catttcttta
    29701 gcagtgtcaa tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta
    29761 acttgctgtt caatcgcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca
    29821 aagcgaagga attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata
    29881 ttgtgaaggg cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg
    29941 cgattgctat caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca
    30001 actgtatgaa gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaatc
    30061 ttacctgact catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa
    30121 aatgacgttt ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca
    30181 ttgtcagcaa cgatagctac caatttagaa gacaattggc tagtattgta gttgaagaca
    30241 ctttctacag tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca
    30301 gctgacgcac gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct
    30361 acagtcatac cagcctcttc agcaatttct tcagtcaatt caactgggaa cccataagta
    30421 tcatagagtt tgaagacatc tgaaccagcg ataacagatt gacctttttc tttcaagtct
    30481 gctacaatgc cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct
    30541 tcgctcttaa cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt
    30601 tccatgattt ttccaacagt tggaaccaat ttgtaaagga aaggctcgtt gattcccaat
    30661 ttttgaccat gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca
    30721 tttcctggaa gggcaccatc accgatggca aatgaaagtg aacggatgtg gtcagcgatg
    30781 accttgaagc tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact
    30841 tcacggataa tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg
    30901 gccaccaaac gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat
    30961 tcgctacgag gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca
    31021 atataacggt cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca
    31081 aaggcttccc cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccgatt
    31141 tcccagaagt tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag
    31201 cggttgtaag aatctttatc gtctggatag taggtcatgt aaagtttttc agcagggaaa
    31261 tcaaaccatt cagggcttat caaaagctca taagcccaag tgatagcttc gtcacggaag
    31321 taatccccga tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc
    31381 cctacgtttt cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt
    31441 tcagggataa tggtcccgtc aaagtatttc ttaagggttg ctaccccaga gttgatccac
    31501 aaaagagttg ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg
    31561 gtcgcccaga aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg
    31621 tctccttatt tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg
    31681 acacagaggg aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta
    31741 gaggtcagat ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc
    31801 aatttgaaat tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca
    31861 tacttatctc gccaaaaggt caacttctta cgaatgacaa aactcgagcc atccagaagc
    31921 tgaatctcaa aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca
    31981 ccagccgcat catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga
    32041 taggcaattt ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt
    32101 tgtttgacaa gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac
    32161 gaagggcgaa aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta
    32221 tgcaattgta tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt
    32281 tctctggact aagacaagtg aaaatcaatt ctcaactttc ttattatatc gtttttttaa
    32341 tcttgcgtca actggaaatg atctccgttg aattagacca attccctaca tctctgatta
    32401 cttttccagg atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt
    32461 actaaacaca gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga
    32521 ctcagttgtt aggtattgtc gatccaagcc atcctttaaa tggaatagta tagcagtttg
    32581 gttaacaatc ataaaggttg gccagaaact tttgttgaaa aaagtagaca ttttcattat
    32641 ttgttgccgc tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct
    32701 agcctcaagc tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc
    32761 ttagtatact actaggcaag caaataagca aataaacaac tagaatagaa aaagataggg
    32821 ctctaaaaac tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac
    32881 gtttatctcc tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt
    32941 ccatttatgg tcaaatttct cataaatttc taggtgtttc atggtttcaa catcgggata
    33001 gaaggcctta tcttcctttg tttcctctgg gagcaattcc ttcgctggta tgtttggtgt
    33061 tgaatagccg acatactccg cattttggag agcattttca ggtttcaaca taaagttgat
    33121 aaaggcatag gctgagtctt ggtttttaac tgttttggga atgaccatat tgtcaaacca
    33181 aagattgctg gcctctgtcg gtaccacata acgtagattt tcattttttt ctaacatttg
    33241 gctggcttca ccagagaagg tcacgccgat tgcaacgtta ttctgaatca tatagccctt
    33301 catctcgtcc gcaacgatag ccttgatatt tggagtcagt ttgtagagct tatccactgt
    33361 ctcttccaac tgctgcagat ccttggagtt gaggctgtag ccgagggaat tgagtcctag
    33421 tcccagcacc tcacgcgccc catcaaagag catgatagaa ttcttatact ccggcttcca
    33481 aaggtcatcc caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa
    33541 ggttccccag aagtaaggga tggagaattt attacctggg tcaaaggact ggttgagaaa
    33601 ctctggtccg atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc
    33661 ttcgtccttc atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc
    33721 accctgcttt atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg
    33781 aattcctgtt tcttctgtaa actgagtcaa gagttcagga tcgatatagt ctccccagtt
    33841 atagataacc aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat
    33901 tccccacaag acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat
    33961 gcttgctcct ccttctcacg agagataaag taataaccta caactaggat aatactaaag
    34021 agaaagacta gagcagacag ggcattgatt tctaaggaaa tccccttgcg agcacgagag
    34081 taaatctcga ctgatagggt tgaaaagcca tttcctgtta caaagaaggt cacggcaaag
    34141 tcatctaacg aataggtgaa ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa
    34201 ggaagcatga tttccttgaa catctgaaat tgactagctc ccaagtcata ggccgcatga
    34261 atcatgtcgc cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg
    34321 gagaaggcca cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta
    34381 aagagaatca agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg
    34441 agtgatagaa aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa
    34501 gtcccgataa tggtcgctat caaggctgat aggaaggcca agaaaaatgt ctgagccaaa
    34561 atcagcatga gtctcccatc tccaaacatg gtttcaaagt gagtccagct aaaacctgta
    34621 aagctattca tatcatcacc agcattaaag gcatagccaa tcaagtaaaa gataggcagg
    34681 tagaggacca gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc
    34741 tttccttagt cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccgatggttg
    34801 aacccatacc atagttgtca ttggttagaa aattctgctc aatagccgtc cccaaggtga
    34861 taacgcggtt cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga
    34921 ccgactgaac cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaagg
    34981 tctcccactt agttgcacca aggtcataac tagcattgat gagattatta tccatgtcgt
    35041 ctaagacatt gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga
    35101 taaaggaaaa atcggtaaaa agcaactgtt gtgaaccaat tccgataaat tccaagaatt
    35161 ggttaataga gccattttga ccaaaaatcc cgataaaagc ataggcttta aggagcaaat
    35221 tgatccaggt aggaaggata atcagcatga gccagagttg acggtgcttg agacgggtca
    35281 aaaagagggc tgttggatag ctgataagca aggtcacaaa ggtcacaatc cctgcataaa
    35341 gcactgagtt gaaactcatt ttgaggtagg tcaagttttg tgacgcaaag taagatttat
    35401 aattttctaa actaaactgg ccttcaatgt tgaaaaagga ttgtccgaaa atcaagacca
    35461 agggtgccaa tacaaagagg gcaatccaaa gcatgtaggg cactacaaag agtttagagc
    35521 ttgttttctt catctctttc ctcctcgatc gcattgatca gacctgcttc ttgctcttcg
    35581 atttccacgt attcttcgat acgagcatcg aactcttctt cggtttcatt gagacgcatg
    35641 atgtggatgt cttctgattc aaagtccaga ccgatttcct cacccacgat ggccttacgg
    35701 gttgagtgga tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact
    35761 ccacggaaga gctgggtgtc gaccttaact tggagtttgc cttcttcagg aagggtaatg
    35821 cgcaagtcct ctggacgaat aacgacctca acaggttcat ttggcttcat cccaccatca
    35881 accgcttcaa agcgtttgcc gttaaattcg accaagtagt cctcaatcat ggtacctggc
    35941 aagatgtttg actccccgat aaaggtggca acaaagtggt tgattggctc atcgtagatg
    36001 tccacagggg ttccagactg gacaatctcg ccatcattca taacgaaaat ccagtcactc
    36061 atggcaagag cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt
    36121 tgtaattcac gcaattcgta ctgcatgtct gttctcaatt tcaagtccag cgctgataaa
    36181 ggctcgtcca acaagaccac acggggttgg ttgatgatag cacgggcgat ggccacacgc
    36241 tgacgttgtc ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc
    36301 atcttgagaa cttccgctac acgctgctcg atttctttct tatcaatttt acgcaagcga
    36361 agtggaaagg caacattttc aaacacattc atatgtggga acaaggcata ggattggaag
    36421 acggtatgta cgtcgcgctt gttggttgga atatcattga tacgaacacc gtctagcatg
    36481 atatctcctg tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc
    36541 gaaccagatg cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct
    36601 ttgagaacct tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt
    36661 ggttttttca attggcataa attccttctt tttcatagat taaccgatcg gggctctgtc
    36721 aggtccccac tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag
    36781 gctttcaccc aagatccgga cttctctttc aagcgtaata cctgagtgtt ccttgacttt
    36841 ttcgataacc gattggatca agtcctcgta gtctttggcc gttccatctg cgacattgat
    36901 cataaatcct gcatgctttt ctgacacttc tacgccaccg atacgatagc ctttcaagcc
    36961 agcttctgaa attaactgac ctgcaaaatg cccgactgga cgcttaaaga ccgagccaca
    37021 agatgggtat tccaaaggtt gcttgagttc acgtaggtgc gtcaagcggt ccatttcctg
    37081 cttgataacc tgatgggttc ctggagctag ggcaaattta actgacaaga caactgcacc
    37141 agactcctga atagctgaat ggcggtaacc aaaagccaag tctttagcag acagggtttc
    37201 gatttctcca tccttggtca agaccttaca agactgcaag atgtgagcaa tctcgccacc
    37261 ataggcaccc gcattcataa agacagcacc gccaacgctt cctggaatac cacaagcaaa
    37321 ctcaaagcca gttaaactat gacggagggc aatgcgagtt gtttcaatca agttagcccc
    37381 agcttctgct tcaatggtat agccatcaac agaaacgtta ttgagcttgt cacacaagat
    37441 gacaaatcca cgaatcccac catcacgaac gatgatattg cttgcattgc caagaaccat
    37501 ccaagggata ttttcttggt tggcaaattt cacaaggcga gccaactcaa aacgatttcg
    37561 tggaaagacc aaataatcag cctctccacc tacttttgta taactatagc tatgcaaggg
    37621 ttcctgaaaa cggatatcaa ttccttctaa gatttcaagc attttttctc ttacagacat
    37681 gtcactcttc cttttccaaa attcattcca ttataccatt tttagagaca tttgacgacc
    37741 ataaaaatac cttgtttgga ttttgcataa gaaaaagagg ttcccccctt tttatgattt
    37801 tttacaaaag atttccttgg ttccataggc gaccagaacg agctccagtg ctagaatcac
    37861 ttcaaccaag actggatttg tcaaccagcc tacttggaaa agagatggtg ccagatcaaa
    37921 gaaggcatgc aagccatagg ctgttaggag ataaatccat ttcttctggc gaacagcttg
    37981 gtaaacccaa actgtcaaaa gtaattggaa accaagcgcc aagattcgct caaaaccaag
    38041 caaataaatc tgccagaccg aaagtgactg aatggttttt aacatatttt cagacagtaa
    38101 ttgcataacc tgtggattct gagtttgaac tgccgaaaga acaatgtaaa gattgagtaa
    38161 actagtaagg cctagaaaaa tcaactccaa gccaccatgc cccagtccat aggccaaggc
    38221 gtctgccttt tccaaacttc tctttttctc aaaccatttg aagaaaacaa gacgagccgt
    38281 ttcctcaaaa aatgctgcca tggctaggcc atagataata tagataagcg gatgatcttg
    38341 caagagggca atactaccgt ctttttgagg gtgcaaaatc aagatatgca ccagtttttc
    38401 caaaatctgc gaagagacaa agaaagcgac tgcacccaag cccaagacag ctagattaat
    38461 ctggtatttc tttttggcaa accaaatgct tcctatcaga aaagctagca acagcaacat
    38521 ggtaataatg atatgaatgg tcattttctt ctcctattct gccttttcaa tatctttttt
    38581 catctcgtca acattgaact tagcaaacaa gtattgacgg tcttggactg ggaaacgttg
    38641 gtccaactgg tcaactgctc ccacctcgat aatgccttcc ttgatgacaa aatccatgac
    38701 atgtccaccg aaggtcaaat catctgatat gaagtgcaga tggtagcctg ccacactgac
    38761 cccatggaaa atctcaggcg tccagaaacc aacaatagtc cccgcaacat tgtcacgact
    38821 atattccggt tgatgggttg cgacatcagc aaacttggta tcgggtgttg acttaggaat
    38881 catacgcaca tgcatatgta aaaattcccc ccgaatcttg atagagcgga aaagattttc
    38941 cccatcataa taagactcaa ttcgttcttt caattccttg tctgtcatct caaagcgctg
    39001 gcggaaaatg acctctgcct gatgcggtac cactgcagcg taaggaataa gggcatctgg
    39061 tgacacttcc acaatttctg gctggtctcc tgaccctttg gcctgataag ccttgccatc
    39121 caagacaatc aattccccat caatggaatc caaggttccc aaaccaaggt caccatgttc
    39181 tagcaattct cctactgtca tagtaccacc ataaaggcca gccatcaagg ctccaagagt
    39241 attgtattga aataatttca ctggttcctg cacgttctta tccattcact ttcttgtctg
    39301 ttattctata aatcttactc ctaagtatac cacatttgcc cctagatgtg aacgagagaa
    39361 acgttctaga cattgccaag aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg
    39421 tctggaatga agaaacaaag caagtacaaa gaggtcgttt cctatctgaa aaatggtatc
    39481 gagtctggac gatttccgac gggtagtcgc ctgccttcta tccgtcaact gagccttgac
    39541 tttcactgca gcaaggacac cattcaacga gccctgctgg aattacggca cgaacaatac
    39601 ctctatgcca agcctcagag tggctactat gtattagaac aagggcaaca tcaagaccta
    39661 gaaatcgagg ttaccgacga acatgccagt gcctatgacg atttccgact ctgtgtcaat
    39721 gaaaccttga ttggccgaga aaactacctc ttcaactact atgacaatca agaaggatta
    39781 gaagacctaa gacagtccat tcacaaactc ctctttgagc aagctctcta ctgcaaggct
    39841 aaccaactag tactgacttc tggaacccaa caagccttat ttatcctctc tcaaatatcc
    39901 tttcctagac aagccaagga aatcttggtg gaacagccaa cctaccatcg gatgaatcgc
    39961 ctcttgattg cacaggggct ggactatcaa acgattgaac gaggcattga tgggattgac
    40021 ttggaggagc tggaaggcca cttcaaaaca ggaaaaatta aatttttcta caccattccc
    40081 cgatttcact atcctctggg acattcctat tctgagcaag acaaacgatc tattcttaac
    40141 ttagctgcca agtatgatgt ctatatcgta gaggacgatt atctgggtga tttggactcc
    40201 aagaagggcc aaaccttcca ctatcttgat acagaggagc atgtcattta tatcaagtcc
    40261 ttctcgacca gcctttttcc tgcccttcgt attacagcac tcattcttcc aaatgctatc
    40321 aaagaagcat ttgtggccta caaaaatatc ctagactacg acagcaacct cattatgcaa
    40381 aaggccctgt cactctatat tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg
    40441 accaatcatg aatcttacca aaaacaaatc gaggaaagga taactaaaac accttgtccc
    40501 cttcctcatt atcccctaca cgatggttta ttgctagacc tgagacagta tcctaaaatc
    40561 gccagtctca aacacagtca actgggcttg gacttctttg aagaggccta tttgagcacc
    40621 tgtccttatc aatttgccaa ggtgtcccta gacaatctgg aaaatgtttt aaactattta
    40681 aaagcagaat tggaatgaca tccaactctg ctttttctta ttcttcaact tcagttagac
    40741 ttgctgcttt ttcaagatag ctttggtaag ttccttcatc cttaagtttt tggatgacct
    40801 tgtctaccac ttctttcaaa tcatcactat tttttctaag agcaacggca ttggcgtcgc
    40861 cgtccttcat cttcaagctg acagttgcga cagctaagcc agcgttttta gcagcataac
    40921 taagtgcaac aggctcatcc atatgaacag catctatttt tccagcctgc aattcattga
    40981 ctgcttcacc catattagtt agggaagtta attgagcttt tggcaattgt tccttgacca
    41041 ttgattctgg aacagtccct ttttgggctg caatattagc actttctagg ctagttaaat
    41101 ccttgtattt ttccacatca gccttacgaa ccaagaaact aatcttgttt tcatagtatg
    41161 ggattgaaaa atcaaagact tcttttctct cgtcagtagc actaattcct gcaactgcta
    41221 ggtcagcctt accagtttga agactggtca aaacattgtc aaaactcatg cttaagattt
    41281 ccaacttaac cccaagttca tcagcgatag cctgagccat gtcgatgtct gcaccgacta
    41341 cctggttctt tccatcaacc aatgattgaa attcaaaggg tgcatagtca ggactggttg
    41401 ccacaactaa tttccccttt tgcttaatgg cctcaacagc tgactgagaa ccattagaac
    41461 ctgactggca agctactaag aagaaacttg caagaaaatt acataaaaca aatatccatt
    41521 tttttgattt cataaataaa caacctttct gttaattttg tataattata acgatatcca
    41581 agttacttgt caagtgtttt ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa
    41641 ggagccatca gttgatttca agctcctttt tatacagaat taaactattt tatagttcga
    41701 caatcttacc tgtttcaaag tagacaaccc attcacagat atttttagca tagtcaccga
    41761 tacgctccaa gtaggaaata acttggaaat aatcacgacc cgtaacaatg gcttctggat
    41821 ttttcttaat ctcttcagtc gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct
    41881 catccatgga ggccacccgg tatgcgtcgt caacagaacc attaagataa agatcaagtg
    41941 ctgcttccac aacgctttta acttcacgtc ccattttttt aatttcttcc tctacagctg
    42001 ggatgcgttc ttcccccttc atacggatgg ttgcctgggc aatggctaca gcgtgatccc
    42061 ccatacgctc cacatctgat acagccttaa ggacagtcaa gactgtacgc aaatcttgag
    42121 agactggttg ttggagtgcg atcatttcaa atgatttctt ttccagtttc acttcgtatt
    42181 catttacttc tgcatcatct tcgatgacct cttttgccag gtcacggtca tgcgtgacaa
    42241 aagcacgtac cgtacgattg atttgtgaga gcacttcttg tcccatagcg tagaactggt
    42301 tatgtaattt ctctaaatct tcttcaaatt gagatcgtaa catctttcat ctccttatcc
    42361 aaattttcct gtaatatagt cttccgtttc cttgtgttgg ggatcaagga acatctgctt
    42421 ggtatcatta aattcaatca aatctccatc taggaaaaat cctgtcttat cagagatacg
    42481 tgaagcttgc tgcatggaac gggttaccag aagcatggtg tacttgtctt ttagaccata
    42541 caaggtttcc tcaattttac cagctgaaat cggatccaaa gccgaagttg gctcatccaa
    42601 gaggatgatt ttaggactag ttgccaagac acgggccacg cagacacgct gctgttgacc
    42661 acctgacaat ccaatagctg aatcatatag acgatccttg acctcatccc agatagaggc
    42721 accttgcaag gctttttcta cggcttcatc cagaacctgc ttatccttaa ttccattgat
    42781 acgaagcccg tagacaacat tctcatagat agtcataggg aaaggattag gttgttggaa
    42841 aaccattccg atttccttac gtaattcaac cgtatctgta cgcggactgt agatgttgtg
    42901 accattgtac accacggatc cagttgtggt cacctctgga ttgagatctc ccatgcggtt
    42961 gagagacttg aggagggttg acttccctga tccagatgga ccaatcaagg ctgtaatttc
    43021 cttaggttgg aaagataggg aaacactatt caaagccttc tttttattat aatatacgga
    43081 caggtctgat acctgtaaaa tcgcatctgt catacggttt cctttctaac caaagtgacc
    43141 agatacatag tcattggtgg actgtagctt ggcattttgg aaaatagttg cagtcttgtc
    43201 atactcaatc aaatcaccca agtaaaagaa gcctgtatag tcacttgcac gagcagcctg
    43261 ctgcatatta tgcgttacaa tgatgatggt aaagtttttc ttgagctcaa acatggtctc
    43321 ttctagttgc atggtcgcaa tcggatccaa ggctgaggct ggctcatcca ttaagaggat
    43381 atctggctta acagagatgg cacgagcgat acagagacgt tgttgctgac cacctgataa
    43441 ggtcaaggct gacttgtgga gatcgtcttt aacctgatcc cagagggcag cctgacgaag
    43501 ggaggtttct acgatttcat ctaggacttg cttatcctta actccagcac gttcatgcgc
    43561 aaaggtaata ttacggtaaa ttgacttagc aaatggattg gggcgttgaa aaaccattcc
    43621 aatgtgttta cgcatttcat aaacgttgat ttctggacgg ttgacatcaa ttccacgata
    43681 gagaatctgc ccagttactt tagcaatatc aatagtatca ttcatgcgat tgagactgcg
    43741 taagtaggta gatttccccg atcccgacgg accaatcaaa gctgtaattt tatttctttc
    43801 aaattgcata tcaatcccct taatggattc atttttacca tagtaaacat ggacatcctt
    43861 agtagaaagg gctacttttt cttcaggaaa ggtaaggata tgcttctcat cccagttata
    43921 tgttgacatg gcttctcctt taggcagcgg ttaatttctt gtgtagatag cttccgaact
    43981 tacgagctcc aaagttaaaa atcaggataa agatcaggag cacagcggca gaacctgctg
    44041 atacaatggt tccatctgga atagtgcctt cactattgac tttccagata tggacagcca
    44101 aggtttctgc ttgacggaag atagagatgg ggctagtcac actgaggata ttccagttag
    44161 accagtcaag agctggcgcc gattgccctg ctgtatagat cagagctgca gcttcgccaa
    44221 agatacgacc agatgccaag acgacacccg ttacaatacc tggaagcgct tctggaataa
    44281 caacatgaac cactgtctcc cagcgagaaa tcccaagagc cagaccagcc tcacgttggg
    44341 tatggtgaac gtgtttcaaa ctatcctcta cattacgcgt catctgaggc aagttaaaga
    44401 ctgtcaaggc caaggcacct gaaatgattg aaaatccata ctcaaactgg actacaaaga
    44461 tcaagtaacc aaagagaccc accaccactg atggtaaaga ggacaagatt tcaatacaag
    44521 tccgcacaaa gttggtaaca ggaccttttt tagcatattc agccaagtaa atcccagctc
    44581 ccatagaaag aggtacagaa ataatcaagg taatgaccaa taggaaaaag gaattgtaaa
    44641 gctgaatgcc aatcccacca cctgcttgaa aagcagaaga ccttccagtc aagaaagacc
    44701 aagagatatg gggcaagccc cgaaccaaga tatagagaat caaggaagcc aagattgtca
    44761 caatgatgct agcaatcgta tagaggacag ctgttgcaag tttatctaat ttcttagcgc
    44821 gcataatttt tctttcctct ttctttcgta atcaatttaa tcacactgtt aaaaactaaa
    44881 ctcatcaaga gcagtaccaa ggccagtgac cagagaacat tattatttac agttcccatg
    44941 acagtgttcc caattcccat agttaatata gaagttaaag ttgcagctgg tgtggtcaag
    45001 gaagttggga taacagctga gtttccgaca accatctgga tagctaaagc ctcaccaaag
    45061 gcacgcgcca tcccaaagac cactgcagtg aaaataccag aacgggccgc cttcaagatc
    45121 acacgccaga tagtctgcca gcgagtggct cccatagcga aacttgcctc acgataataa
    45181 cgaggaaccg cacgcaagct atccgttgtc ataaaggtta cggtcggcaa aatcatgaca
    45241 aagaggacgg aaatccctga caaaatccca aaaccagtcc caccaaagac actgcgaaca
    45301 aagggaacga cgacttgcaa gccaataaat ccgtacacta ctgaaggaat cccaaccagg
    45361 agttcaatag ctggttgcaa aatcttcgcc ccttttggtg atacttcggt cataaaaact
    45421 gctgcaccaa tagcaaaggg tgttgcgata agggctgaga gaatggtaac gataaaggaa
    45481 cccaaaatca taggaagggc accaaattct ttactagaag gattccaagt tcctcccaaa
    45541 agaaagtcaa agatattcac accattgaca aagaaggtcg acaagccttt ttgcgctacg
    45601 aaaaccaaaa tcatggccac aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct
    45661 tttcctaatt tctccagacg agaattcttt gatggaagca acattttctt agctaattct
    45721 tcttgattca ttattgtctc ccttccaaca ctgtcacagt tccggcagca tctttttcaa
    45781 ccttcatttc cttaatcgga atatacttca atcctttgac aatcccttct tgggtctcat
    45841 ccgagagaac aaaattgaga aattctgcag ctaactcatt gggctgcccc aatgtataca
    45901 tatgctcata agaccacaag ggccaattat tgctacttat attttctgga cttaagtcat
    45961 agccattcaa cttcatgctt ttgaccgaat catctatata ggtaagagat aaataagaga
    46021 tagctcctgg actttttgat acgattgatt ttaccgctcc atttgaatcc tgctcctgac
    46081 tttgcatggc agactgacct tccataatga cagtatcaaa ggtagcacga gagccagagc
    46141 cggctgcccg attgataaca gagatgggta agtccttacc accaacctct ttccaattgg
    46201 ttacctcacc tatgaagatt tgacgaagtt gctctgtcgt taggttatca acatcaacct
    46261 ccttattgac aatcagagcc aagccagcta ccgcgacctt gtggtcaaca agagcagaag
    46321 catcaattcc gtctttttcc tcagcaaata catctgagtt tcctatatca actgccccag
    46381 actgaacctg ggacaagcct gtaccagaac ctcccccttg gacattgacc gtttttccaa
    46441 catggatcgt gccaaattca tctgccgcta cttcaaccaa gggttgcaag gcagttgagc
    46501 caacagccgt tatggattct ccacgatcaa tccagctagc acagcctact aaacaagccg
    46561 tcagccaaaa agcgataaga gacagagcaa gcttttttct ttttttcact gtttttctcc
    46621 tcgaaaataa ttatgaatac tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga
    46681 attcttacca aatttctgcg caattgatta tttatataat ataggctata ttactctttc
    46741 ctaacctcct tttttcatat gtggataaaa tctcttgtct atcccttccc ccattgtcac
    46801 ccattatagt catttcgtgt ctctttttcc cctttttaat gcaagggaaa ttactctcct
    46861 tagatgataa tccaaaagct agaaaggtat ctcaaatctc tctactctcc cagactagtt
    46921 tacaactaaa aggaaaagat tctattttat gagaaatcta gtttacaagc ggtaagaacg
    46981 ctaataacta aacttcttgt actctttgaa aatctcttca aagcagtgtt ttgagctatc
    47041 tatggctagc ttcctagttt gctctttgat tttcatcgag tagtaaaact acatgtgatg
    47101 gcaatcaaga tatcaagaat catcctacta aaaaaatcca tactttcact ataacataga
    47161 ataagatatt tgactagcat tttcatttga atctgaggcc ttttggaaaa taatttttca
    47221 aaacatttcc agtaaccttt gcaaagccca agccattgcc tttaaccaaa acttggtacc
    47281 aaccatttgg cagactttct gccagctgaa cggtttctcc agccgcatac ttgacaaact
    47341 cttcttggcc aatttcaacc gactgttcga cctgactcgg tttcaaggct aaaccaagag
    47401 cgaaactggg ctcaaagcgt ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa
    47461 tcttgagctt ccctaaatct ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa
    47521 tctgcaagat acccggtaga ttgaccttca aatggttttg ggcaaattcc tgccacaagg
    47581 caacttgttc acggctgagg ttactcttac ttgccttaaa tttaggagct ggattgttac
    47641 ccttaaactg tagatgggca acaaactgac cctctccctt aaactgatga gggtacatcc
    47701 gagccgtttc tggcaggtca ataccagcta ccattccatt gatatgctct actggcaaca
    47761 agtcaaaatc atactcttcc agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc
    47821 aggtacaggt cgaataaacc agacgaccac cttcagctaa catggtcact gcatcctcca
    47881 gaatttctct ttgcaagcta gcacattgac tcggataatc taagctccaa tagtccatag
    47941 catcaggttg cttacgaaac attccttcac cagagcaagg ggcatcaaga acgattaagt
    48001 caaaatagcc tttaaagacc ttgaccaagc ggtcggcaga ttcattggtc accacgacat
    48061 ttgtcgctcc aaaacgctcc atgttttcaa ccaaaatctt agcccgtttg cttgaaattt
    48121 cattggaaac aagtagcccc tcccctgcta gataggctgc cagttgagtt gatttgcccc
    48181 ccggtgcagc agccaagtcc aagaccttca taccaggact gggttgggct acttgagcca
    48241 ccatttgagc agcaggttct tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc
    48301 ctgaaacctt cccatagtgg ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt
    48361 gctcttcttt taagggattg acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa
    48421 gaaaatctct tgcctcatct cctagtattt ctttatattt ttcaacaaat ccttctggaa
    48481 atttcattta agttcttttc ctttcgtaaa tataggactg aatttcctcc tgcatctcaa
    48541 gaggcaccat catgaccggc tgtctggttt gaaaatcagg agcttcacca aaaagggtca
    48601 caacccgata gcccagactt tcccctaaaa tactagctgc ggcataatcc catggttgca
    48661 gataggtgag ataggtcaac aaacgccctg acaaaatctt ggcaaaacta atggccgcac
    48721 ttccatagac acgaacacca agaaccgctc ggctcaaatc agccagcccc cattcattgg
    48781 tttccagcat accactattc cctgcaatga gaaaatctcc aagtggttta gttttaaaag
    48841 aagctagggg cctatcattt agacaaactg gaaattcccc accaccgtgg taacaatccc
    48901 ctttgaccac atcataaatc agaccaaact gtccctgacc attttcaaaa taagccatca
    48961 taacagcaaa atcttcctgc tgggctacaa aattattggt accatcaatg ggatcaatga
    49021 cccaaacctt gccctcttga accgaggctc gcagacaacc ttcttcagca caaatcttat
    49081 cctcaggata acgggacaaa atctcaccaa ccaagagttc ctgaacttct ttgtccagtc
    49141 tggtcaccaa atctgttgga gaggacttgg tttcaacacg caagtcttcc tgcatatggt
    49201 caagaatgta ctgacctgct ttcttaacaa gctctttagc aaattcaaat ttacttttca
    49261 agagaaatct ttcctttccc tttttctttg gcagcctgaa ctgctcggta aagtgaatag
    49321 ccactaaccg tttcaaattc tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc
    49381 gctttgaatc ccttatagga gtccagtaac tttttagcct ctactttgcc ttcataggca
    49441 gcttcaacat cattaaaaaa agaaagcact gaagcaagtt cttcagtgct ccacgacaaa
    49501 tctagtgggt aactatactg tttgttcatt aactaatacc agctctcatt cttgcttctt
    49561 ttagttcttg cttacgataa ctacgaggga gaaaagcacg aatctcatct tcattaaaac
    49621 cgatttgcat acgcttggca tcaataataa ttggacgacg caaaagacta ggatactgct
    49681 caatcaaatg aagcaattcc gataccgaaa tactctctac atcaatattc aatttttgaa
    49741 aaatttttga acgagttgaa atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt
    49801 gttgcaattc ttttcttgtt aaaggactgg tcataatatt gtgttccaca aagggaacct
    49861 tatgtttttc taaccaggtc ttagccttac gacatgatgt acagctcggt gatagaaata
    49921 gtgtaatcat gcttttctct tcttatctat actttgctac ttctattata caaaaaaata
    49981 aggcgcttga ctagggattt ttagaaaaaa agcctatttt ttcaggaaaa ataggctgtt
    50041 tgcgaacgat tgacacaatt ggatttggtt aattcactct taacgatggt tttaaacgat
    50101 atatatattt atatatgtaa attaaaaaca tctttccttt cacttcctac gacttttcag
    50161 acacagatag ccaaagaagc tttcatagag ggcaaaaaag aggaggaagg catgaagaaa
    50221 gaaggtctct ggcaaaatca taataacagg atccttggct ggatcaaaaa gccaggtatc
    50281 atctcccaca aagagaattt gatggaaaag agtaaagaat tggtcaaaac caatcaaaac
    50341 tcccccaagt ccaatcatca caggtaagac tactagagcc aggagacttt ttcgataaag
    50401 agacaaaaag tcctttttca caatcctatt gacaaagaca tagaaacttg gcagtgtcac
    50461 tagagctact agctgaacca aatgaaagag attcttgacc actgcgaaat ggtgcaaacc
    50521 agctgctgac gaacgaaaat caggcatctg taagacctga ctaaaaggat tggtcagata
    50581 attcatcaag atatgaaaat tgtattgaat ggtttctggt tttagataga ctcgattcgt
    50641 taagtttagc cactgaatct ccataggata gaaaatccaa gccagataaa tggtcagaag
    50701 gattgagagg gagaggagaa agagcataga gccccaaaag gtcaatttag ttttcatcaa
    50761 aatcccactc cgcaaggcta gaaaccacat gtgtcggtgc gattggcagg ccagctactt
    50821 cttctgcctt ggtaaaacct gtcgtcacca agagcgttgg aatgccattg tcaatcccag
    50881 cccggatatc agtcaaataa ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta
    50941 agtgctcaac tgccttgtcc ataatgatgg cattgggttt tccgatataa accggcttca
    51001 ctcgtgtcgc tacttcaagc agcgtaatca gtgagccagc acctggcaaa agaccacgtt
    51061 ccgtcgggat gttgaggtca ggattggttc cgataaaatg ggcacccttt tgaatagcaa
    51121 gagttgctgt ggcaaatttt tcatagtcga cttgccaatc cagaccaact accacgtagg
    51181 caggtttttc cttgtcttcc acataaccag ccgccttgat ggcttccttg agtcctgctt
    51241 ctccgacaac atagacggtc ttttcaagcc ccaaatcatt catatagtcg atggttgcca
    51301 aagtcgctgt gtagacagtc gataggggcg tatcgatatt aaaattctga gccaacatct
    51361 ccttaacact ctctggagtg cgggttgtat tgttggttac aaagagatag ggaatgtccc
    51421 gcttttgcaa ttcatgaaca aaagtctctc cagcagggat ttggtctttc cccttataaa
    51481 tggttccgtc taaatcaatt aaatagcctt tatatttcat ctatttctcc ctaagccttt
    51541 tttatttctt gccaagtaat gattgcttgg gcattgataa ccccatcact tgtaatttca
    51601 tgcttgcttt ccagtccagt ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact
    51661 tccttgacat acttgaggtt gattttcttg ggaatatagt gggtcaaaaa atccgctccc
    51721 atgacctcaa aaatccagtc taagtattta ctgttattga catgaccatt catatccaag
    51781 tcgtaaaaac gaacatggta atccttgctg atcgactctt ccaaggactc atacttcggt
    51841 ccacggataa gttttttatc aaaatcagac tggtaaggag ccacaatctc aggttcaaca
    51901 gcatggactt ttcgactgtc gcggtccatg agaacaaagg tcgccatcat atggatgagc
    51961 tcctgccccg cttcattata aatagtaaag cgacggtagc aaaaaagtcg attgtagctc
    52021 aaggcttccg tttcgatggt aatttcttcc gcaaaacgag gcaaacgaac cacctcaata
    52081 tcatattcta cgataatcca gaccagatta tagtcttcca aaatggcctt atcactaact
    52141 cccagttcaa tcgactgcat ccctgaaact tgcagtgaca gcaaaattac atctggaagt
    52201 ttgatatgac cgttcatatc agccatatca aaaggaattt tcattttcat ttgataagtt
    52261 aagcccatga tcctactcca aaataaatcg ttccgctaca gtatctccca aaaagagacc
    52321 tctctttgtc atgcgaacgt ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc
    52381 tctgacaatt tctccataaa gtccagcaaa agactgtcca aatttttcct caaatcgcgc
    52441 catggaaacc ccggatttct tgcggagtcc caagaacatt tcttcttcca tttgctcctt
    52501 ttgactcagg tgatcttctg taatacaagc attgccttcc tcaaccgcac tgagataatg
    52561 acgaatggga ccatgatttt tatagcgtac tccattgaca taaccagatg cccctgcacc
    52621 aataccatag tattcagcat tgtcccagta catgagattg tgacgacttt caaagccggg
    52681 tttggagaaa ttagaaatct cataatgctc aaaacccgct cgctccagct ctgcaatgat
    52741 gtactcaaac atctccgctt ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat
    52801 ccgattcata aagaccgtat ggttttctaa aatcaaacta tacaaactca tgtggggaat
    52861 atccagtcca atggctttag ccacattttc ctttacttgc tccatggtct gaccaggcag
    52921 agcataaatc aaatcaatgg agatgttgtc aaaaccagcc agtttcaggc gatcgatatt
    52981 ttcataaata tccttctcca aatgactgcg cccaatcttt ttcaacatct tatcatcaaa
    53041 ggtctggaca cctagcgaaa cacgattgac agccgaattt ttcaaaacag ctatcttatc
    53101 cgcatccaaa tcgcctggat tggcttcaat ggtcaactct tctaagacag acaagtccaa
    53161 gtttttagtc aagccattca gtaaaacctc cagttgcgga gccgacaggg ctgtcggtgt
    53221 tccaccaccg atataaaggg ttgacaactt ttcaatatca taagaacgaa actcttccag
    53281 cagatgctct aaatagctgt cgactggctg atttttgatg aagacctttg aaaaatcaca
    53341 ataataacaa atctgggtac aaaatgggat gtgcacatag gctgacgttg gttttttctg
    53401 catagtaatt attataccac aaagactaga ttccagataa aaatcaccat ccccagatac
    53461 atagtccgtc cggagatggt gatggtttat tcttctgtta tatcaatcac aatctcttct
    53521 gagtcatcaa gagcttcggc tttttcttgc cattgctcct tgagattatt taattgattt
    53581 tttgatgctt ctgtcgcttg aaaagcatag gatttagctt gagcaagtat actgtccaca
    53641 gtgatttcac ctgactcaac ctgttctttt gttttcagaa caaaatctgt agcctgctcc
    53701 ttaacttctg tcagtttttc acagacttgc tccttggcat actccggatc ttctctcaaa
    53761 tcatctagaa aatcttgagc ctgactgcaa acttgtttgc ccttatcact tgttaaaaac
    53821 aaggcaagag ctgcacctga aacggttcct aaaaggattg aggataattt acccataagg
    53881 attctccttt tttatttttt gaaaaattta cttgcaagac gaagagctga cagacttgca
    53941 ccagtcttga gtgtttttga accagctgat gaagctttct tgctcaagac acgcgcatgg
    54001 tcattgaggt ctgaaacaga tagagataaa tctgcaacag cactgaagag tggatcaatc
    54061 gtagccacct tgacattgat atcatctgcc aagacattga ccttagccaa caactcattg
    54121 gtgtgatgca aggtcacatc cacatctgaa gtcaaggttt taatcgtctt ttctgtttca
    54181 tcgatgacac gaccaagctt ttgtacagta atgatcagat agaccaaaaa gacaatcaaa
    54241 gctagggcaa caagaatata tgcaacttct aacatttagt tttcctcctc tgtaatatag
    54301 taaggggcct tctttcgatt ttgataaata acgatcatta taccgagacc gataaggaca
    54361 actgacagcc attgggacac tcgaaggccg aagaacatga gactatctgt tcgcatacct
    54421 tcgatgacca tacgaccgaa accataccaa atcaagtaaa aggccgtgat atgacctcgt
    54481 ctgagactct tccatttccg tctaaaaatc agaatcaagg caaagccaag cagattccat
    54541 agagactcat aaaggaaagt cggttgacgg tagctcccct caatatacat ctggtcacgg
    54601 ataaagccag gtagataatc cagattatcc actgttgcac cataagcttc ttggttaaag
    54661 aaattacccc aacgccccaa actttgagca atcataacgc taggcgccgc aatatctaga
    54721 aaatcccaag tattgatgag tttacggtca gcaaagatat agagcacaag agccccagtt
    54781 atcaaaccac cgtaaatggc caaaccacca ttccaaatgg caaaaatctc tcctaaattc
    54841 tgactatagt aatcaaatcg gaaaataaca tagtagagac gagctcctaa aatagccaag
    54901 ggaaaggcta ctaagataaa atctaaaata tcgtctggta tgatcttctt tctaggtgct
    54961 tctttcatgg tcaaataaac cgcaagaatc aagcctgtca caatacataa ggcataccaa
    55021 cgaatggcta ggggtcctag ttgaatagca attggatcaa gcattttgca cctcatttcg
    55081 agcgatgaga cttgtcagtc gttcgtcgaa caaacgggtc gcatcaaagc ccatttcctt
    55141 ggcacgataa ttcatggcag ctgcctcaat cacaacagag atattacgac ctgttttaac
    55201 tggaatacga atacgaggaa tggctacgcc agaaacttca agttcctctg cattgtttcc
    55261 aagacgatca aaggtcttat gcgtatcgta attttccaaa tagacagcaa gctgaacctg
    55321 tgaagaatcc ttgacagcac tcgcaccgta gagactcata acatcgataa taccaacccc
    55381 acgaatttca atcaagtgtt tcaaaatttc agctggttca ccccagagag taatctcatc
    55441 cttggcaaag atatcgacac ggtcatctgc taccaaacgg tgaccacgtt tgacaagctc
    55501 aagacctgtc tcgctcttac caattccact atctccctga atcaagacgc ccatcccata
    55561 aatatccatc aagacaccgt gcacactggt acgttctgcc aaacgagaat ccagatagct
    55621 agataactct ccagataaac gactggtagc tgcacggctg gttaaaatag caatcttaca
    55681 ttctctagca gcctttaaca tctcctctgg aaccaccaaa ccacgggcaa caatgactgc
    55741 tggtgtctca gatagaaaca tttttttcaa aacttcataa cggctgttgg aaggcatgct
    55801 gatcagataa gaccactcct tcatccccaa aagttgaatc cgctctggtg tgtagtagtc
    55861 aaaatagcct gtcatttcaa gaccaggtcg cgtaatatcc gctgtattga tttccttttc
    55921 aagcaactct ggttcaccat agacaatatc tagtctaagc ttttcaatca cttcttttac
    55981 taaaaccgac atcatttcct ccttcaaatg agttcttctc tctattatat caaattgtag
    56041 gaggaagttt cagatttttg caggctttgg agtatttatt taaaagaaaa aggactagat
    56101 ttctctagcc tttcattcca attagaattt tttacgttta cgagctactt ctgatttacg
    56161 tttacgtttt acagaaggtt tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc
    56221 agctttagta accgcacgtt tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg
    56281 tactactgtt ttagacattt ttttcactcc cttcaagttc gatatctatt tcattctaca
    56341 cgtaaaagga ataattgtca agcaaaaatg ctgtttaatt ttgatttttt cttccattta
    56401 tacaagagtt gaaagcgttc aatagaacat tgctttttta tttttcgagt aagctaagcg
    56461 cttcagcatc tgcgatgatg gttacatcag ggtgattttg gaggctactt gcaggtaggt
    56521 tctcagtcac tgggccagat actgttccgg caatggcttc tgctttcgac tcaccgtaag
    56581 caaaaagaat aatagacttg gcatccaaaa tgtttttaat ccccattgaa atagcttggg
    56641 ttgggacgtc ttcaatcttg gcaaagaagc gtgcattggc ttcgatagta gactggtcaa
    56701 gttctactag atgcgtttga ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc
    56761 cattgcgacc gattcccaag atttggaggt caactggatg gtcagccaaa atttggttgt
    56821 agcgttcaac ttcagcttca gcattgtcct taacaccacg aggcaagaaa ctttctttaa
    56881 atggtttttg gttgaacaag ttttcttgca tgaagtaacg ataagactgt ggattgtccc
    56941 catcaaggcc cacatactca tcaaggttga cactggttag atttgaaaaa tcaaggtcac
    57001 tctcaacaat ttccttgtaa aattcaagtg ggctacttcc tgtcgcaagt cctagagttt
    57061 gagctccatt agccaatttt tccttcaaaa tctcaaaagc aacttttcca ccttgaactt
    57121 ggttttcaac tttaataact ttcatctttt catcctccat ttctgtctat atttattata
    57181 tggtatagac caatttttgt caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa
    57241 acttgaaacg tgttataatg gataggatta gaacttagaa agaatgaaca gatatatgaa
    57301 tacagctgat tttgatttcc acttgccaga ggaattgatt gcccaaacgc cccttgaaaa
    57361 acgtgatgcc tccaaactcc tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca
    57421 cttccactct attattaata tgctggaacc tggtgatgcc cttgtcatga acgacacccg
    57481 agttctccct gcccgcctct atggtcaaaa agtggagaca ggaggtcatg tggaacttct
    57541 cctccttaag aacactagtg gagacgagtg ggaagttctg gctaaacctg ccaaacgcct
    57601 caaggtcggt actcgtatca gctttggtga tggccgcctc agcgctgtcg ttacagaaga
    57661 attgacccac gggggacgca ttgtccgctt tgaataccaa ggaattttcc tagaagtctt
    57721 ggaaagtctg ggagaaatgc ctctgccacc ttatatccac gaaaaattag atgaccgtga
    57781 acgttatcaa accgtctacg ccaaggaaag cggctctgct gcagcaccga ctgctggtct
    57841 tcacttcacc aaagaactgc tggcagaaat ccaagctaag ggtgttcatc tagtctatct
    57901 gactctccat gtcggactcg gaacctttag acctgtttct gtggataatc tggacgaaca
    57961 cgaaatgcat tcagagttct atcaactttc tgaggaagct gctgccaccc ttcgctctgt
    58021 caaagaaaat ggtggtcgcg tcatcgctgt cggaaccact tctatccgca ccttggaaac
    58081 tattggttcc aagtttgatg ggcaaatcca agcagattct ggttggacca atatctttat
    58141 caaacctggg tatgagtgga aggtcgtgga tgccttctca accaacttcc atctgccaaa
    58201 atcaactctg gtcatgttgg tttctgcctt tgcaggccgt gaattagtct tagatgccta
    58261 ccaccatgcc atccaagaac actaccgctt cttcagtttt ggtgactcca tgtttattta
    58321 ttgagaaaga atttctctaa atcttctaat accaataaat cgctaagata ttatttcaaa
    58381 gaacatctac aattgaaact ctagctagct gtagaagacg cctagtacat tgaaattaaa
    58441 atgcttcccc ctagcttcga aaatattgcc atagattgcg ttgactctcc aaattgattc
    58501 atctatattt tatttcagct tcctatattt tcttcgctgt ttgtaaatca aaatgcaaga
    58561 cacatgagta gcaccatatt tgttactctt atctgtcctc tcaagagact attatgagtt
    58621 atttcagaat cattcactac tttgaccctg actctcctta gtctcaaaat caaagactta
    58681 tactcttcaa aaatctcttc aaactgcgtc aacgtcacct tggattatat atgtgactga
    58741 cttcgtcagt tctatctaca acctcaaagc agtactttga gcaacctgcg actagttttc
    58801 tagtttgctc tttgattttc attgagtatt aaacaaaaag tgaacaaatc tgaattctaa
    58861 tgtacagaag actaggcttg ttcacttttt tatagtcgct ataagatgac cttatctata
    58921 gctttttata tataattata tataattata tattcagaca tactattatc aattttgtcg
    58981 cagggaggaa tctgttaacg cacccattca ccattatcat tgactctata gccatctata
    59041 cttgtattga ccgctaactc acccgatgca tttacataat accatttacc accaacttgg
    59101 aaccattgat tgactttcat agaaccgttg ctgttgaggt agtaccatga accattaact
    59161 tgtacccaac ctgttgccat ggaaccatca gtattataaa aataccacat accattttct
    59221 tgtttccagt ctgttgttgg agcaactgct ttagctggtt ctactgctac atctgttcct
    59281 tggttagatg taacagatac aggatacgaa ggaatagatg attgctcagg aacaacaact
    59341 ttttcaggtt ctctcgtccc tctccttata cgtcttttta ccatctcttt agtaatttga
    59401 cgagaagtag tttcttcaat tgttccatca cgttcatcta cagtatagat tgtagtaaga
    59461 gtaatttacc aatttctcct acttcttcta cttcttgact tttatcaaga gttgggtcat
    59521 cgagatattc tgtttcgatt ggaatttctt ggacaagaac ttggggcttg gttccttttt
    59581 taacaactct tgtttgagag tcttcttttt gacttaaagt actctcagtt acttgtccac
    59641 tctttccatc tacatcataa gttatcgttg taactgtttt cccattcttt cctagagtaa
    59701 tctcttgctc ctgtcctgca gaaaggtcat tgtctgcttc atatttagta gcacatggaa
    59761 caagaacttc ttcaaccttg cttttagctg gaactttgat aactgtatcc gtggcttgtt
    59821 ttctatcaac agtaacctgt tcggtaacat gaccagtctc tggattaaca tcgtaggtcc
    59881 ttgtcgtagt tacatagcca tcctctccat caattgtaac aggattttca ctacggtctt
    59941 ttgtttcatc tttttcataa cgaattcgcg tacttgaaat tttcttggtt actaccttag
    60001 gtttagtcgc tacttttaca ataatacccc cattgtcagc gtcatcatac tctattccct
    60061 cttctttatc tctagtatca tctctgacat attgaatccc atcagcagca tgaacaaaac
    60121 ttgtattcag attcctccta aaaataaagt tagcccgatt accgcagaac caaaaatctt
    60181 tccgagttta cgtaatgcat agcgcttatt agtattagat tttgccatta catcctactt
    60241 ctagtatagc atcttttcta tcaaacgtta aacaatatac tttatatata aaatagactt
    60301 agaatgatat attgattatt gaactaacac tttaactata tcgtaatcaa tctcatatat
    60361 aaaggattgc agacatctta tctaaataca tgctaatata tttagataca aacattccaa
    60421 cttgataatt ttcactcatc tttcatcatt ccttatacaa ctatgcagta taaatagaat
    60481 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
    60541 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
    60601 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
    60661 tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa ccagattaaa
    60721 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
    60781 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
    60841 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
    60901 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
    60961 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
    61021 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
    61081 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acgatgctga
    61141 tatctcctat cacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
    61201 gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg atatcaggat
    61261 ggggatccca ggtaacatag gtcagattct cagcagatga agcccaattt tgaaacatgg
    61321 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
    61381 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
    61441 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
    61501 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
    61561 gatgaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc taaactcggg
    61621 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
    61681 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtccccggac
    61741 cttcttcgct ttctcatttc taggctcagg gtaaaaacct ccaatggagg tttttactcc
    61801 caaaagtcat caaatacagt gatgggtaag tggcgtttgt gttggccttt gtgccaccag
    61861 ttttcaatgg tcgcttgagc ttctgggctg attgttttgc cttctaggta gtcgtcaatc
    61921 tctgcgtagg tgactccaag tgcgacttcg tcagctaggc ctggtttatc ttcttctagg
    61981 tctgccgttg ggattttttc ataaagggct ggctctgcgc caagtttctg caagagctgt
    62041 tttccttggc gtttattgag gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac
    62101 ttggtaaaga aacctgtgat attttccgcg gcgtggtctg ttccaatgac cgctccgcta
    62161 tgggaaccag caagggcata ctgagcaatc atacggcaac gtgccttgat attccccttg
    62221 ttgaagtctg aaacaggact acctgtcgct tcaactgcag ctgtcatggc atcagctgat
    62281 tccttgatat tcacaaccaa gctgacatct ggctggatga aggctagggc tttttgagca
    62341 tctgcttcat cagcttgcac tccgtatggc aggcggacag cgataaattt gtagctgtcg
    62401 tctcccgttt cagctcgcag ttcttccata gctaattgcg ccaaacgccc tgccaaggtt
    62461 gagtcttgtc ccccagaaat ccctagtaca aaggttttta ggaagggatg ttttttcaga
    62521 tatcttttta agaaatcaat agaacgacgg atttcttcct gggcatcaat cactggtttg
    62581 acacccagct cttggataat cgtttcttgc aaactcattc ttcttctcct tcaccaaggg
    62641 cttccttgcg catcttatca atcaagtcca tcttatcttg ccatacatca cgcgccaaat
    62701 ccactggata gtgctgcgga ttgagcacac gcttatactc atcccacaac ttgtcaaatt
    62761 ccttacgggc ataatcctga atgtcagtca aactaggcaa gttgtaaact aatattcctt
    62821 ctttgaagat atccaccaag agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt
    62881 atgtataggt cggatggaac atcttgattt ctgtcatgtc gctaatatcc acaccatcat
    62941 aagtgatata gtcgccttct gacttgcctt tttcacgact ggtaatgcgc cacacctgct
    63001 tcttacctgg cgtagagact ttttccgcat tattagacag cttaatcgta ttgcgcatct
    63061 gaccagtttc atcttcgatt gcaacaatct tgtaaactgc accaagagct ggctggtcat
    63121 aagctgtaat cagcttggta cccacgcccc agacatcaat cttggccttt tgcatcttga
    63181 gattgaggat ggtattttca tctagatcat tagaagcata aatcttagcc tctgtaaatc
    63241 cagcctcatc cagttgctga cggactttct tagaaatgta ggcaatatcc ccagagtcaa
    63301 tccgcacacc cataaagtta atctgatcac ccagctcacg cgccacctga atggcagctg
    63361 gtacaccgat gcgaagggtg tcataggtat ccacaagaaa gacacaattt ttgtgggtcg
    63421 cagcgtaagc cttgaaagct tcatagtcat tgccataaac ctgtaccaag gcatgggcat
    63481 gggttcccaa aacaggaatg tcaaagagct tacccgcacg cacgttgctg gttccattgg
    63541 cgccaccaat cacagctgcg cgtgttcccc agatggccgc atccatttct tgagcccgac
    63601 gtgtcccaaa ctccatcaag ggttcatctt cgataaccga acgaatacga gctgccttcg
    63661 tcgccaccaa ggtctggtag ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt
    63721 gggctagagg tccttccacc tgcacaatcg gttcattagc aaaaaccaaa tccccttctt
    63781 gggcagaacg aacggtcaac tccaacttga aattgcgaag gtaatccaag aacgccccat
    63841 gataaccaag cgactccaaa taggctatat cactatctga aaaacgcaag tcttcaagat
    63901 agttcacaat tctttccaaa cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc
    63961 ggaaatacac ctcaaagacc gccttcttat tgtgaatccc ttggtcaaag taaacctgca
    64021 tcatgttgat ctggtacaag tccgtgtgca atgtcaaact atcatctgga tacatacttt
    64081 tcctacttcc ttagctagaa acccatgaaa attttcaaga actttcatgt attccaataa
    64141 attagtacta ttatatcaca ttttagctgg attgagaaaa gagtaacaag ctattctcca
    64201 ctctccaatt catccatatc ttgttcaaat tttttctgag cccattcgcc atagctctta
    64261 agaccaagat tgccaataaa gacccacgga aggtaaatga cataagtaat gacccaagca
    64321 gacaggtatt taaaattcaa aggattgtgc tgataaattt ctatgttgaa ttgataattc
    64381 tgcaacatca aaagagccgt aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa
    64441 tgaaaacgac tatagtaggt cactcccaga taacgggcac ggttaaaaaa gtaaaatgtc
    64501 cctatgatga taacgattag cagcatatta gaattaaaaa ggcttggtgc taatactgaa
    64561 atgatataag ataggagcga caaagcgatg cagatataga aactttcaga gcccgcttta
    64621 ttgatcagtt gttcttctct ttcgtctagt aattgataat aataaaatct atttttcatc
    64681 ttcttcctcc caaaatagtt ggtctagggt tttccctaaa catctgcaaa tagactggca
    64741 gagcgagaga ctgggattgt attttcccgc ctctatcaaa ccaatagtct ggcgtgtcac
    64801 cccgacagcc tctgccagtt gaccttgtgt taaatcacgc tctacccgag ctaattttaa
    64861 ttttaaattt ttagccacct tcgtcctcct tatagtttta atactcatct actcttaaaa
    64921 aatccaaact aacacaagct gtcagctatt tattataacc ccattaattt gcactccagt
    64981 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    65041 ttttattttt atttttattt agcattagct ttagtaggat cttttaaacg agtttttatt
    65101 gcttatattt cttgtttagt ttagttggtt tcatatcact attatataat gcttttggaa
    65161 atgagcctag tataagtcaa aaacgtgttg aatagtgaaa gcaggagcat accaatatga
    65221 ctgatttttt actgtatttt aggtatgcaa aataatacta aatgaaacta aaatgaatac
    65281 ttcttgcttt tcttgagagt gaaaaagtac aataaaatga ttgtagtgat tgccacaaga
    65341 tactgactaa tagacatcgt atttgaagcc acaaatacag gtaaagaata aaaaggaaga
    65401 agttcaattg ctttgataaa caaatcatta ctagtattca tcatcgtatt taatatatta
    65461 gggataaaga gtagtaaagc tactaaaata ccaagccact tttcaatttg tataaataga
    65521 aaatgtaaga taatagaaaa taaaacagga ctaacaaaat tgtattgact acaatagcta
    65581 ctataaaatc taatcctaaa tcacctggaa tacccaattt acttactatg actagtaaca
    65641 caatgccatt caaagcataa ataatggaag ctgagatggc atatagaaaa ttagacaaga
    65701 cgtaaatcat ccgattcttc tgttgattat aaaacaaact taccgtattg tgggaaaaat
    65761 ctctactaat gactcgatta gcatgtataa cagccattaa cataccgagc atagcataaa
    65821 agtttgaaat atgcttaata ccaagcgatg tcccttaagt ctttagaata aagaatgtaa
    65881 gcaatatcgg cggtataaac gagataagca aactgagata tattacgcct gatgaatata
    65941 aatttcttat attttcctta agaaaattca atttatttaa tttcatgatt ataaccttcc
    66001 tttattttga acgatttagg taaatatctt taagcgtctc ttttttagtt tcaaaatcaa
    66061 ctactttaat agagttttcg ttaaaaaatt taaaaagctc actactttga atattcccag
    66121 acatagtaat tctcaatccc tcttcctgaa caatatcccc aaattcttgt ttggtaatga
    66181 aaatgtctct atctgtagct gatgaaaaag ctatctcaaa caagaaatta tgactatctt
    66241 ttcctacttt ttgaaatgtc aaaagcccgt tctccaagaa aagaactctc tcacaaattt
    66301 cttcaatgtc ttctaattta tgactcgata ttaaaattcc cacgttttca tttaaagcta
    66361 atttttttag aaccgctaaa actatttgtg atgattcaat atctaaacca ttagtcggtt
    66421 catctaaaat caatatatca ggttccgtaa cgagagttag aagcaaagct aatttttgtt
    66481 ttgtacccaa agaataggtc tttacttttt tattaataga ctgagtcaaa tctaactctt
    66541 ggatcaaact tctaaatctt tcttgattgt agtcaacacc atataaattt gacaaatatt
    66601 ttaaattctc taaacctgtt ttagataaaa ataattttgg ttcttcgatt aaatacccaa
    66661 cattatcact gcttataata ttacctgaag tcggttgatt attttgaaca agaattttca
    66721 tcaacgtact cttaccaact ccatttctcc ctactagacc aacaatttca cccttgttaa
    66781 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttttat
    66841 tcctacatta atattcatgg tttactcttt gattcttatt gagtttaaaa agttgcgcct
    66901 ctttttaaca atattatatc atatatctaa cttgatgcaa tatataaatg tcattttgta
    66961 aaattttatt aacaaaaaac tctctacaac aaaataatag agagttttct gcttattaat
    67021 ttttttcaaa atatctaaga catcgcttct ttgtttaatt tgataggctt tgatacctaa
    67081 gctccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc atcaagggat
    67141 ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcataa ttttttaaaa
    67201 ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta taaagattga
    67261 taagcccctc tttaagagct cgaaccatgt ctcctgattg aacgggttgt gggactttaa
    67321 tagtcaatag ttccattgga tttggagcgc ggtcgatttt attgcctttg gcatcatgca
    67381 aatcttcaat ataggtttca aaatgacgga aacctggacc ataaaactca acttggtccc
    67441 cttcgttaat gacgtttcgt tgacgaatag ttgctgtttg tgccgcatca tcataagaaa
    67501 ccacttcagc gacaaacttg tactcaggga ttttacgacg agcaccaaac aactgctcat
    67561 tttcagatgg tgtaccatag taaaatcctg tagccagttc acgttgggca accttccaca
    67621 tctcgtccac caagtcttgt ttgatagctt caaacttttc aggactttca agataggcat
    67681 ccacagccgc cttgtagcag ttggttactg ttaatacata gtgaatagac tccatacgtc
    67741 cttcgatttt tagactgtcc acaccatttt caatcatatc tggaatgtgg tcaatcatag
    67801 acatgtcaac ggctgacatt gaaaattctt ctggaatctc accctgcaaa ctcttacgtt
    67861 ctttcccaaa tggcatatcg taaaggtcgt atttccaacg gcatgactga gaacatccac
    67921 cacggttggc atcacgcata ctctagacgc aattctaatc ttttagactt tgcataataa
    67981 ctgttaaaca gttgaaaatt aacccagttt taacaagtag tcaaaatacc agataaagga
    68041 aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat cttgatatta
    68101 tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt tctcctactt
    68161 caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca acctggatta
    68221 aagcgagaaa aacaaaaggg gcaacttaag ctttctttat attgcttata tgttagtagt
    68281 tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg atacggctca
    68341 aaagggtgat tttctaatga atcatgacac cgataacaag catagtactt tttacaaaca
    68401 tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct tgtttgctta
    68461 tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct cgaactgtga
    68521 cgctggttcc atcttcctta taaagattga tgagtccttc ttttaatgca cgaaccatgt
    68581 ctccagcttg aacaggttga ggcaccttaa tagtcaatag ttccattgga tttggagcgc
    68641 ggtcgatttt attgcctttg gcatcatgca aatcttcaat ataggtttca aaatgacgga
    68701 aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcg
//
LOCUS       DAVBCR010000002        63812 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCR010000002 DAVBCR010000000
VERSION     DAVBCR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.002"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            212..3982
                     /gene="spuA"
                     /locus_tag="VQ508_000071"
     CDS             212..3982
                     /gene="spuA"
                     /locus_tag="VQ508_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2489138.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSNPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWIKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            complement(4082..5131)
                     /locus_tag="VQ508_000072"
     CDS             complement(4082..5131)
                     /locus_tag="VQ508_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2489139.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ508_000073"
     CDS             complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ508_000073"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2489140.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGTDNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ508_000074"
     CDS             complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ508_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2489141.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWDRDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8728..10581)
                     /locus_tag="VQ508_000075"
     CDS             complement(8728..10581)
                     /locus_tag="VQ508_000075"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2489142.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALIEKLEASLMGDGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ508_000076"
     CDS             complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ508_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2489143.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11875..12678)
                     /locus_tag="VQ508_000077"
     CDS             complement(11875..12678)
                     /locus_tag="VQ508_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2489144.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12687..13445)
                     /locus_tag="VQ508_000078"
     CDS             complement(12687..13445)
                     /locus_tag="VQ508_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2489145.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13676..14248)
                     /locus_tag="VQ508_000079"
     CDS             complement(13676..14248)
                     /locus_tag="VQ508_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2489146.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ508_000080"
     CDS             complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ508_000080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2489147.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15361..15453
                     /locus_tag="VQ508_000081"
     CDS             15361..15453
                     /locus_tag="VQ508_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2489148.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(16080..16496)
                     /locus_tag="VQ508_000082"
     CDS             complement(16080..16496)
                     /locus_tag="VQ508_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489149.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(16508..17203)
                     /locus_tag="VQ508_000083"
     CDS             complement(16508..17203)
                     /locus_tag="VQ508_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489150.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(17397..19898)
                     /locus_tag="VQ508_000084"
     CDS             complement(17397..19898)
                     /locus_tag="VQ508_000084"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2489151.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(20353..21441)
                     /locus_tag="VQ508_000085"
     CDS             complement(20353..21441)
                     /locus_tag="VQ508_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2489152.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(21459..22127)
                     /locus_tag="VQ508_000086"
     CDS             complement(21459..22127)
                     /locus_tag="VQ508_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2489153.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(22146..24584)
                     /locus_tag="VQ508_000087"
     CDS             complement(22146..24584)
                     /locus_tag="VQ508_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2489154.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(24735..26033)
                     /locus_tag="VQ508_000088"
     CDS             complement(24735..26033)
                     /locus_tag="VQ508_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2489155.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(26050..26358)
                     /locus_tag="VQ508_000089"
     CDS             complement(26050..26358)
                     /locus_tag="VQ508_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2489156.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(26404..26724)
                     /locus_tag="VQ508_000090"
     CDS             complement(26404..26724)
                     /locus_tag="VQ508_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2489157.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(26919..27899)
                     /locus_tag="VQ508_000091"
     CDS             complement(26919..27899)
                     /locus_tag="VQ508_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2489158.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(27912..28658)
                     /locus_tag="VQ508_000092"
     CDS             complement(27912..28658)
                     /locus_tag="VQ508_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2489159.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            28780..29556
                     /locus_tag="VQ508_000093"
     CDS             28780..29556
                     /locus_tag="VQ508_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2489160.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            29631..29828
                     /locus_tag="VQ508_000094"
     CDS             29631..29828
                     /locus_tag="VQ508_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2489161.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            30045..30641
                     /locus_tag="VQ508_000095"
                     /pseudo
     CDS             30045..30641
                     /locus_tag="VQ508_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            30717..31166
                     /locus_tag="VQ508_000096"
     CDS             30717..31166
                     /locus_tag="VQ508_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2489162.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            31179..32270
                     /locus_tag="VQ508_000097"
     CDS             31179..32270
                     /locus_tag="VQ508_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489163.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKESLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDIRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            32260..33912
                     /locus_tag="VQ508_000098"
                     /pseudo
     CDS             32260..33912
                     /locus_tag="VQ508_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(34015..34707)
                     /locus_tag="VQ508_000099"
     CDS             complement(34015..34707)
                     /locus_tag="VQ508_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2489164.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(34951..36288)
                     /locus_tag="VQ508_000100"
     CDS             complement(34951..36288)
                     /locus_tag="VQ508_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2489165.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINCAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(36298..36564)
                     /locus_tag="VQ508_000101"
     CDS             complement(36298..36564)
                     /locus_tag="VQ508_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2489166.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ508_000102"
     CDS             complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ508_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2489167.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(37227..38162)
                     /locus_tag="VQ508_000103"
     CDS             complement(37227..38162)
                     /locus_tag="VQ508_000103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2489168.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ508_000104"
     CDS             complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ508_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2489169.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ508_000105"
     CDS             complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ508_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2489170.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ508_000106"
     CDS             complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ508_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2489171.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ508_000107"
     CDS             complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ508_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2489172.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(39465..40103)
                     /locus_tag="VQ508_000108"
     CDS             complement(39465..40103)
                     /locus_tag="VQ508_000108"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2489173.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ508_000109"
     CDS             complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ508_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2489174.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ508_000110"
     CDS             complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ508_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2489175.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ508_000111"
     CDS             complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ508_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2489176.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ508_000112"
     CDS             complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ508_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2489177.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ508_000113"
     CDS             complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ508_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2489178.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ508_000114"
     CDS             complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ508_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2489179.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ508_000115"
     CDS             complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ508_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2489180.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ508_000116"
     CDS             complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ508_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2489181.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ508_000117"
     CDS             complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ508_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2489182.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ508_000118"
     CDS             complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ508_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2489183.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ508_000119"
     CDS             complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ508_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2489184.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ508_000120"
     CDS             complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ508_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2489185.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(46636..46842)
                     /locus_tag="VQ508_000121"
     CDS             complement(46636..46842)
                     /locus_tag="VQ508_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2489186.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ508_000122"
     CDS             complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ508_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2489187.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ508_000123"
     CDS             complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ508_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2489188.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ508_000124"
     CDS             complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ508_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2489189.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ508_000125"
     CDS             complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ508_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2489190.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ508_000126"
     CDS             complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ508_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2489191.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(49527..49823)
                     /locus_tag="VQ508_000127"
     CDS             complement(49527..49823)
                     /locus_tag="VQ508_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2489192.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ508_000128"
     CDS             complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ508_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2489193.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ508_000129"
     CDS             complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ508_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2489194.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ508_000130"
     CDS             complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ508_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2489195.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(51887..52504)
                     /locus_tag="VQ508_000131"
     CDS             complement(51887..52504)
                     /locus_tag="VQ508_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2489196.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLSVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ508_000132"
     CDS             complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ508_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2489197.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(53084..53590)
                     /locus_tag="VQ508_000133"
     CDS             complement(53084..53590)
                     /locus_tag="VQ508_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489198.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(53634..53774)
                     /locus_tag="VQ508_000134"
     CDS             complement(53634..53774)
                     /locus_tag="VQ508_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489199.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ508_000135"
     CDS             complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ508_000135"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2489200.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(56113..57651)
                     /locus_tag="VQ508_000136"
     CDS             complement(56113..57651)
                     /locus_tag="VQ508_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2489201.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(57688..57831)
                     /locus_tag="VQ508_000137"
     CDS             complement(57688..57831)
                     /locus_tag="VQ508_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489202.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ508_000138"
     CDS             complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ508_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2489203.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(59587..60144)
                     /locus_tag="VQ508_000139"
     CDS             complement(59587..60144)
                     /locus_tag="VQ508_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2489204.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(60141..61391)
                     /locus_tag="VQ508_000140"
     CDS             complement(60141..61391)
                     /locus_tag="VQ508_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2489205.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(61599..61904)
                     /locus_tag="VQ508_000141"
     CDS             complement(61599..61904)
                     /locus_tag="VQ508_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2489206.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ508_000142"
     CDS             complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ508_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2489207.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(62343..62609)
                     /locus_tag="VQ508_000143"
     CDS             complement(62343..62609)
                     /locus_tag="VQ508_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2489208.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(62696..63265)
                     /locus_tag="VQ508_000144"
     CDS             complement(62696..63265)
                     /locus_tag="VQ508_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2489209.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ508_000145"
     CDS             complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ508_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2489210.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        18883 a        14185 c        11396 g        19348 t
ORIGIN      
        1 ttctgaataa aaatagctgg gaacacatca tcttcctaat aatacggctt ggcttatgtg
       61 acatttttat ctccgtcatt tatactgata tataaaatag tacagaaaac ttctcaaaag
      121 taggaatttt ttcacaaatc aaagaaaacg cttgcatatt gttttactac atgctatact
      181 gatatagaaa tttattgatt ggagaatcat tatgagaaaa acaccatctc acactgagaa
      241 aaaaatggtc tacagcatcc gttccctcaa aaatggaact ggttctgtcc ttatcggcgc
      301 aagccttgtc ttgcttgcca tggctacacc aactatctca tccgacgaaa gtacaccaac
      361 cactaacgaa cccaacaaca gaaatacaac cacccttgcc caacctctta ctgatacagc
      421 agctggctct ggtaagaacg aaagtgatat ttcttcacct agaaatgcaa acgcttccct
      481 agagaaaaca gaagaaaaac ctgcaacaga gccaactact tctactagtc cagtaacaac
      541 tgaaactaag gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact
      601 tcctgaagaa aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc
      661 tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta
      721 ttacctagat gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa
      781 tacagctgga aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa
      841 cgaggcttgg ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac
      901 tgttcgcgtc aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg
      961 gggagatgta aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac
     1021 aggcaaatat ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt
     1081 tttattacta gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa
     1141 gttcacagat ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta
     1201 cacaaatcca tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc
     1261 tagcattgaa agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca
     1321 ctccaacatc actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga
     1381 agctggtaag aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt
     1441 tagctacaat tccgaccaat tcactaccaa aacaagctgg cgcctgaaag atgagacata
     1501 cagctatgat ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac
     1561 cctttggtca ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga
     1621 caaagtagtt ggaactgtcg ctctcgaaaa aggggaaaga ggaacttgga aacaaactct
     1681 agacagcaca aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat
     1741 cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg
     1801 gaatagcgac gatgccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga
     1861 tccagctaaa ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg
     1921 tgaagacgcc gttatctacg aagctcatgt gcgtgacttc acttcagatc ctgccattgc
     1981 aaaagacttg accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct
     2041 caaagacttg ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa
     2101 tgaattgaaa aaccatgaac gcttgtctga ctatgcttca agcaacagca actacaactg
     2161 gggatatgac cctcaaaact acttctcctt gactggtatg tactcaagca atcctaagaa
     2221 tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat
     2281 gggagctatc ctagatgtcg tttataacca cacagccaaa gtcgatatct ttgaagacct
     2341 agagccaaac tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg
     2401 tggacgcttg gggacaaccc accatatgac caaacgcctc ctagttgact ctatcaaata
     2461 cctagttgat acctacaaag tggatggatt ccgtttcgat atgatgggag accatgacgc
     2521 cgcttctatc gaagaagctt acaaggctgc acgcgccctc aatccaaatc taatcatgtt
     2581 aggcgaaggt tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca
     2641 agattggata aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct
     2701 caaatctggt tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt
     2761 taacactatc tttaaaaatc tcattgctca gccaactaac tttgaagctg acagccctgg
     2821 agatgtcatc caatacatcg cagcccatga taacttgacc ctctttgaca tcattgccca
     2881 gtctatcaaa aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttgag
     2941 acttggaaat ctcatggtct tgacagctca aggaactccg tttatccact ctggccagga
     3001 atatggacgt accaaacaat tccgtgaccc agcctacaga actcctgtag cagaggataa
     3061 ggttccaaac aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta
     3121 cttcatccat gactcttacg attctagtga tgcagtcaac aagtttgact ggactaaggc
     3181 tacagatggt aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat
     3241 tgcccttcgt caatctacag atgccttccg acttaagagt ctccaagata tcaaagaccg
     3301 tgtccacctc atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg
     3361 ctaccaaatc actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa
     3421 agctcgcgaa tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc
     3481 agatgaaaac caagcaggac cagtcggaat tgccaacccg aaaggacttg aatggactga
     3541 aaaaggcttg aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac
     3601 tagccatgag tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca
     3661 agacccagct ccagaagcta gacctgattc tactaaacca gatgccaaag tagctgatgc
     3721 ggaaaataaa cctagccaag ctacagctga ttcacaagct gaacaaccag cacaagaagc
     3781 acaagcatca tctgtaaaag aagcggttcg aaacgaatcg gtagaaaact ctagcaagga
     3841 aaatatacct gcaaccccag ataaacaagc tgaacttcca aatacaggaa tcaaaaacga
     3901 aaacaaactc ctatttgcag gaatcagcct ccttgcgctc cttggtctcg gtttcttact
     3961 aaaaaataaa aaagagaact aaactagccc tcctatagaa aaatccccca agcattatag
     4021 ctcgggggat taatttttgt acaatatttg ttgtcctaat aaacttgatt aggatttttt
     4081 attaagcctc tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga
     4141 gttcaatagc tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga
     4201 agcggtccaa tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat
     4261 tgccaacaaa catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt
     4321 cctgccagtg cggacggtct ttggaaatag catccaccac ttgcttagtc ggatggaaat
     4381 aatctttcac cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt
     4441 tcaagtcttt gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact
     4501 ttttaaaata acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag
     4561 caatcttcac tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct
     4621 gaactggacg aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt
     4681 ctaacttggt cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt
     4741 cgtagtcttt caagtcataa ccaaacagag ggaaagattc cgtgaaagat ccccttccag
     4801 ccataatctc cgctcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac
     4861 gaatcgggtc catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat
     4921 tgactgcccc agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt
     4981 gctcgccaat cccatatacg tccaaaccaa ccttgtcagc cagctcaatc tctgccacca
     5041 actggcgaat gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt
     5101 ccccaaatgt tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtcctt
     5161 caatttgaaa aattattcta acacgaatct tgagtacaag caaccaattt gctcacaaga
     5221 aaaagcctag ataactagac ttttttagct tattctaccg ttactgactt ggcaaggtta
     5281 cgtggtttgt ccacatcgag gccacggtgg agggttgcaa agtaagcgac taattgcgtt
     5341 ggtacgacca ttgaaattgg tgagaggtat ggatgtacgg tcgtaaggac gatatcgtcg
     5401 gtatctttgg ctacattctc ttctgcgata gtgaggactt tggcaccacg ggctgcgacc
     5461 tcttggatat ttccacgagt atgattggca agaactggat ctgacaagag agccaaaaca
     5521 ggcgttcctt cttcaatcaa ggcaatggtt ccgtgcttga gttctcctgc agcaaagcct
     5581 tcacactgga tataagaaat ctctttgagt ttgagacttg cttccatggc tacgtagtaa
     5641 tcttgaccac gtccgatgta aaaggcgtta cgagttgttt caagaagttc acgaaccttg
     5701 gcttcaatgg tttctttctc tgaaagagtt gattcgatag actgagctac gattgacaat
     5761 tcatgaatca ggtcaaaggc ttgcgcttta gcattaccat ttgcttctcc gactgctttt
     5821 gcaaggaagg caagggctgc gatttgcgct gtataggctt tagttgatgc cacggcaatt
     5881 tcaggacctg cgtgaaggag catggtatag ttggcttcac gtgagagggt tgagcctgga
     5941 acatttgtca ctgttaagct tggaattccc atttcattag ccttgaccaa aacttgacga
     6001 ctatccgctg tttcaccaga ttggctgata aagatgaaga gtggtttctt gctgagaagt
     6061 ggcataccgt agccccactc agatgagatg ccaagttcaa ctggtgtatc tgtcaattct
     6121 tccaacattt tcttagaagc aaatcctgca tggtaagatg ttccagctgc aaggatgtag
     6181 atgcggtctg cgtcttgaac agccttaatg atagcaggat caaccactac ttgaccagca
     6241 tcatccgtgt aggcttgaat gagtttacgc ataacagttg gttgctcatc aatttcctta
     6301 agcatgtagt aaggataagt tcccttaccg atatctgaca agtcaagttc cgcagtatag
     6361 ctagcacgtt cacgactgtt accatcatag tcttgaactt ccacgctatc agccttgacg
     6421 attaccaact cttggtcatg aatttccatg tattggttgg tttcacgaat catagccata
     6481 gcatctgagc agaccatatt gtagccttcc ccaagaccaa tcaaaagtgg agatttgttt
     6541 ttcgctacat agatgacatc tggattttca gagtcaatca aggcaaaggc atatgaacca
     6601 cggataatat gaagagcttt tttaaaggct tcaagaactg agagaccgtc ttcttccgca
     6661 aattttccaa tcaaatgtac ggcgatttcc gtatctgttt gccctttgaa gtggtgccca
     6721 gcaaggtatt cttctttaat ttcaaggtag ttttcaatca ccccattgtg aaccaagaca
     6781 aaacgttctg tctcagagcg gtgtgggtga gcattgtctt ccgttggttt cccatgagtt
     6841 gcccaacgag tatgtccgat accagttgtt ccctcaacac cagctgtctt agcagacaat
     6901 tctgcgatac gaccaacagc cttcaccaaa tggttatcag taccatctag gacaaaaatt
     6961 cccgcagaat catagccacg gtattcaagc ttttcaagcc cttgaatcaa aatatcagtt
     7021 gcatttgtgt ttccaacaac accaacaatt ccacacatag tatatacgac acaggcaagc
     7081 tgtgctttct ccttaaaatt ggtatagtct aattcatctt ttatagaatc agcaaaaata
     7141 gtatatactt gtttctttca cttgtcaaga gtaaaaattg gtatagttca aattaagctc
     7201 ctgtaagcaa aaaactctga ccgattggga taatcagtca gagtcctttt taaaatccat
     7261 tattatcgct taattctttg aaccagtggc ctgatttctt cagacgacgt tcttgcgttt
     7321 ccaagtctaa ttcgaccaaa ccatagcgat ttttatagct gttgagccat gaccagcagt
     7381 caataaaggt ccaaatcaag tagcccttac agttggcacc atcttcaatg gcacggtgaa
     7441 gttcacgaag atgacctttt acaaagtcaa tacggtaatc atcttgaatc attccatctt
     7501 gacggaattt ttcttctcct tcaacaccca taccattctc agtcaacatc cactcaatat
     7561 tgccataatt ttccttgata ttttgggcga tgtcataaat tccttgctca taaatctccc
     7621 aaccacggtg agaattgatt ttacgtccag gcatcacata aggctcgtaa aaatgttctg
     7681 gtaagagtgg actatctgga tgcttagcaa atcgaggagc cataacacgc aaaggttgat
     7741 agtagttcac accaaggaag tccaccgtat tatcacgaat gagttccaac tcttcctctg
     7801 tagcatcagg taaaagaccg tgttcatgca agatttctac caactcctgt ggataagtcc
     7861 ccaagacaga tggatctaag aaagattggg cctgaaaaag ggccgcaata cgagctgcct
     7921 tgacatcagc aggatgctgg ctacgtggat aagccggtgt caagttgagg acaatcccaa
     7981 tcttggaatc aggcaaaagt tcatggcaag ccttaacagc ccggctgctg gccaattgtg
     8041 tatgataggc taccttaaca gctgcctctg catccacctt atgtggataa tgggcatcat
     8101 aaaaataacc aaattctaca ggaacgatgg gctcgttaaa ggtaatccat tgatccacta
     8161 aatctccata agtctcaaaa caaaaacgag catagtcttc ataggctgag actgtcgcct
     8221 tattttccca accatcacca tcctcttgaa gggcaaaagg taaatcaaaa tgatagagat
     8281 tgactaacag acgaattcct ttagccttaa tagcctcaaa gaccttacga taaaaatcca
     8341 caccttgagg gttgactttt ccacagcctt gtggaaaaat ccgtgaccac tgaatagaag
     8401 tccgaaaggc tgtgtgacca gtctctaaca aaagctcaat atcccgatcc caattttcat
     8461 aaaaagtcga tgtcttatct gaaccaatcc cattatagta acgatttggc tccacttgga
     8521 accagtaatc ccagagattg tctcccttgc cgtcaccagc tacacgtcct tctgtctgcg
     8581 gtccagaagt agaggatccc cagacaaaat cctttggaaa tcttagcata catttacctc
     8641 tttatctact catttctccc attatacaga aaaaacaagg taaaaactag ttacattttt
     8701 tccttgtttt tcttctgatt atagttttta tttcttgctt aggatttcaa gcgtttcaag
     8761 cacgttatct gcatgaacct caatggtgtc accagttgcc ttgatcttaa cttctacaat
     8821 gccatcggcc gcttttttac caacagtgat acggattgga agaccaatca agtcactatc
     8881 gctaaattta acaccgacac gttcgttacg gtcatctgtc aagacttcat aaccatctcc
     8941 catcaagctt gcttcaagtt tttctatcaa ggcttgcgct tcttcatcct tgacattgac
     9001 agtaatcaaa tgcacatcaa atggtgccaa ttctttaggg aaattgattc cccaagcgta
     9061 acggtattca ccttttggcg ttttgttaac aaagaggcga gcgtgttgct ccatcactgc
     9121 tgaaagaaga cggctgacac cgataccgta acatcccatg atgattggca cagcacgacc
     9181 attttcatcc aagacatctg ctcccatgct tgctgagtag cgagttccga gtttgaaaat
     9241 atgaccaatc tcaataccac gcgcaaagtt aaggacacct tgtccatctg gggaaatttc
     9301 accctcacga acttcacgga tatccacata ttctgcagta aaatcacggc ctgggttcac
     9361 accagtcaag tggtagccat cttcgttagc accgacaact gcattgcgaa catcttgcac
     9421 cttacgatct gcaataattt taatattctc tggcaaacca actggtccaa gtgaaccaaa
     9481 tcctgcttga acaacattcg ccacttcttc ttcgctagca acgtcaaaga aatctgctcc
     9541 taagtggttt ttcaacttga cttcgttgag ttggtcattt ccaactagaa gggctgcaac
     9601 aagctcacca tctgcaatgt agaagagggt tttaatcgtt tgttcttctg gaacattgag
     9661 gaaagctgca acttcatcaa ttgatttaac atctggcgtt gcaacacgag taacttcttc
     9721 ttcagcgaca acacggttgc ttggtttgta ctcgtttgtt gccatttcta agttagctgc
     9781 atagctagac tcacttgagt aagcaatggt atcttcacca gagactatcc atttgagcaa
     9841 ttctgccttg atttcttctt gcacttctgc aggaatttcg tcaaatgagg caactgactt
     9901 gtccaagaca acccagcggt caaggtctgt acgagcagat gtaatggcca taaattcttg
     9961 gctatcctta ccacccatgg ctccaccgtc accaataata gccttgaagt ctaaaccact
    10021 acgagtgaaa atacgctcat aggctgcttt gtactcatca taaacactat ccaaactatc
    10081 atagttagcg tggaaactat aagcatcctt catgataaat tcacgtgtac gaagaagtcc
    10141 attacgtggg cgtttttcat cacgatactt gggctgaatt tgataaaggt tgagtggcaa
    10201 ttgcttgtaa gatttaacag aatcacggac aatagctgta aaggtttcct cgtgagttgg
    10261 acctaagata aagtctgatt tttcacggtt ttttagtttg taaaggtctt caccataggt
    10321 ttcgtaacga cctgattcac gccacaattc tgcactaaga agggctggag ccaacatctc
    10381 aacagcacca atcttttcga attcttggcg catgatgttt ttagcttttt caatcacacg
    10441 gttggcaagt ggtagataag aataaacacc tgctgaaact tggcgaacat aaccagcacg
    10501 caacataaga gcatggctga taacttgagc atcgcttggc atttcgcgaa gcgttgggat
    10561 aggcatttta ctttgtttca taatattcct cgattatcta aaaaagagtc gcataatgtc
    10621 attccaagtc acagcaatca tcaagacaac catgatgacc actccggcca aggtgacata
    10681 ggtttcaatt tcttgtttca atggtttgcg gcggatggct tctaggatat tgagcacaat
    10741 cttaccacca tccaaggctg gaatcggaat aagattaaaa atcccaatat tgatggaaat
    10801 cattgccaag aagtacaaga tattttcaat tccattttta gcagcatcac tacttgcctt
    10861 aaagatagca acaggtccac ccaacttgtt caaatccggt tggaaaatca gatttttcag
    10921 agctgagaga attcggagag ctgagtcagc agcagttgta aaaccaccta caaacatgga
    10981 tagaaaatct gacttaaccc ccggttgaac acctagaagg taacgacctt gactatcttc
    11041 gggtgtaaca gtgacttgtt tgtcactccc cttttcagaa atagtcacat ccaaagtcgg
    11101 tgccgtctta tctttggttt ctgtttccac agcttggatc aagctttccc agttgctaac
    11161 ctcatgtgag ccaatcttgg taatttgtgc cgtttctggt actcctacct tggccaaggc
    11221 accttggggc atgatatgga actgattggt atcaacatct ctgacaccac cctgcataaa
    11281 gattaaaacc caaaaaacaa cgacacctaa gataaaattg ttcatagggc ctgcaaaatt
    11341 ggtaatcagt ttgccccaga tagtcgcatt ttgatattga acatctaaag gtgcaatccg
    11401 aacctcagta ccatctgctt ccacaaccgt tgcatcgtga tccactgcaa atgttttttc
    11461 ttcttccaga accaatcctt tgataaagag cttgtcttca aaatcaaact gggtcacctg
    11521 catagggagg gctgtttgat ccaatttttt acctgagaga ttgatgcgtt taaccttacc
    11581 atcatcagca agtgtcaaac taacaggcgt tcctgtcttg atttcagttg tatcatcacc
    11641 ccaaccggcc atgcggacat agccacccag aggcaagatt cgaatggtat aggccgttcc
    11701 atccttgcca atgtgagcaa aaattttagg tcccataccg atggcaaatt cacgtactaa
    11761 aatccctgat ttcttggcaa agtagaagtg cccgaactcg tgcaccacta caataatccc
    11821 gaaaaccaga ataaaggtta aaattccgag catagcgttt cctccgtctt ttgattaaaa
    11881 gagtccaaat aagtgcatga ttggaaatac aagcaacata ctatcgaaac gatccaaaac
    11941 accaccatgt ccagggataa atttcccaga atccttaaca ccaaaatgac gtttgatcga
    12001 actttctagt aaatcaccaa attgtccagc aatgctaaag aaaatagcaa agactgacat
    12061 cttgtaaatt ccatatggaa gagcaactgt actgtcaact atcataaaga taatggttac
    12121 taaaattgct cctaaaatac cacccaaggc accctcaagg gttttattag gcgataccct
    12181 tggtgctaac tttcgtttcc catagttcat cccaacaaga taggcaccac tgtctgtcgc
    12241 ccagacgata cacaaggcta agagagcctt gtccaaacct gcaacacgag catctagtaa
    12301 agcattaaat ccaaagccca cgtagaagct catagcaaga gggaaaaccg catcctcaat
    12361 cgtataagac ttgctaaaaa cggtcgttcc taacatgatt gaaatcaaaa cactataggc
    12421 aaccacattc ccatcaactg gcaaaaaagt caggtaattc tccaagggaa tggtcaatgc
    12481 aaaggttgca aagagggtca agaggccctc catcgtcatg gtctctagac ctctcatctt
    12541 caaaagttca tgcatggcta gcatggctat gattccgatt gctatctgaa gcaagaggcc
    12601 cccaatcatt aaaattggta ggaaaatagc cagggcaatc cctgcaaaca aggttctttt
    12661 ctgtaaatcc tgggtcatat ttcctcctaa actcctccaa atcggcgatg acgacgatta
    12721 taggcaagaa tagcttcctg caaggccgct tcgtcaaaat caggccataa ggtgtccgta
    12781 aaataaagct cactataggc tccctgccat ggaaggaaat tgctcaaacg caattctcca
    12841 ctagtacgga taatcaagtc tgggtctcgt aagtccttag gcaaatgctg ggtaaagaga
    12901 tagttaccaa tcaattcctc tgtgatgtca cctggattga ttttggcatc taaaacatcc
    12961 tgggaaatca acttaagcgc ctgtgtaatc tcagcacgtc caccatagtt aagagcaaaa
    13021 ttaagaatca atcctgtgtt gttcttagtc aattcctcag ccttggttaa agcttcaaag
    13081 gtttgcttag gcaggcggtc tgtctcccca atcatttgaa tcttaacatt attcgcatgt
    13141 agttccggga cataattatc ataaaactct actggcaagt tcatgataaa cttgacttcc
    13201 tgatctggac gggtccagtt ttccgtagaa aaagcataga ccgtaataac cttgacgccc
    13261 agtttgttgg ctgccttggt cacggtttgc aatgcttcca tgcccgcctt atgtccaaaa
    13321 actcgcggtt gcatacgttt tttagcccaa cggccattgc catccatgat gatgccgata
    13381 tgagcaggaa cctgtgtcgg aacctctact tccacagcct tatctttctt aaaaaatcca
    13441 aacatgatct tattcctatt caaaaatcta tcgttttatt ataccatatt tccccatttt
    13501 cttctatcac taagctattt attctcaggc accaagccca tttttcaaaa aaataagccg
    13561 cctgattggg cgactttatt tttataggga gattattatg aaaaagtttt aggagtttaa
    13621 gttaaggtct tcttaactta tgaacttagt gtacactccc tagcttaaag tttccttaag
    13681 tatttttaaa aatcaaattt ttccatttct cctgccaatt tttcttggat aaacgtgttt
    13741 gatagagttc cattcggtct tcattttcta agaaatgagg agttggacga acttgaaaat
    13801 tcaaaatatc ctccaaacca taaggtgcat agagttcaaa atctgattct tcattcaagc
    13861 gcagtccaac tgtcgtacac cgttctggat acttactcat agcatcacga gaactggtat
    13921 aggaagcagt gtgaggactg tgctgatgca tatagacctg atttttcaat tcccactggt
    13981 actgaggaaa atcctctcgc agctttttct ccagtaataa ggtttcctca taagaaaaat
    14041 ctggatcaaa gaaaatcaca tctatatctg tttcatgatc aaaaggggat ttgtctgaca
    14101 aaagattcca aatgaaattt ctgacagaac ctgctgccaa ccacgagtct ttcagaccaa
    14161 ggtctcggat gatcgtcaga atggccatca tatctggatt ttctctaaaa gcctctaaga
    14221 tttcttgctt atttttcact gtattcataa cctaagtgct catatgcctt agcagtcgcc
    14281 acccgtccag accgtgtccg catgataaaa cctttttgaa tcaagtaagg ctcatacata
    14341 tcttcaactg tctcacgctc ttcggcgata ttcacagaaa gagttcctag accaacaggt
    14401 cctccactgt acatctcaat catggtacga aggatttttt gatccacata gtccaaacct
    14461 tcatggtcaa catccagcat agtcaaagcc ttatcggtaa taatatcatc aattacccca
    14521 ttccccatta tctgggcaaa atcgcgcacg cgcttgagga gacgattggc aatacgaggg
    14581 gtcccacgac tacgtagggc caactcagat gctgcctcat gagtgatttc catctcaaaa
    14641 atatctgccg tccgctcgac aatttctgtc aagtcagcat gggcataata ctccatatgg
    14701 cctgtaatcc caaaacgtgc ccgtagcgga ttggagagca taccagcccg agtcgtcgca
    14761 ccaatcaagg taaaaggtgg taactccaaa tgaacactac gactaccctc accagcccca
    14821 atcataatat ctatgtagaa gtcctccata gcactataaa gcacctcttc cactgacatt
    14881 ggcaaacgat ggatctcatc aataaaaagt acatccccag gctctaactc attcaaaata
    14941 gctaccagat ctccggcttt ttcaatgact ggacccgacg tctgcttaag attgactccc
    15001 agttcgttgg caataacaaa ggccatggtc gttttcccga gacctggagg cccaaataag
    15061 agcacatgat ccagcgcttc atcccgcatt ttggcagctt caataaagat ttgtagctgg
    15121 tccttgacct tatcctgtcc gatatattca cgtaaatact gaggacggag cgtgcgttct
    15181 actaactcct catcccccat tatctcatta tctaaaattc tactcatggc tctattatat
    15241 caaaaaacca agccacaaac aaaaaagcca cctgattggg tgactcctaa gtttagcact
    15301 tatgtggtat aatattctac gacactgcta caccgcctac gaaaggaggt gagatagccc
    15361 atgatggaat tagtactcaa aactatcatc ggaccaattg tggtcggtgt cgttcttcgt
    15421 atagtcgata aatggctaaa caaggacaaa tagtgtcaaa aaagacctca agcttatttg
    15481 gtcgtgagct tggggtcttt tctagcctat gatatagaac tagtactcaa ttccttttta
    15541 ttatcccata gttcacgaat tttgtcaaag gtttacattt tcttcaaccg ctgtacgaca
    15601 agacggttaa gattaagaga acgttaggga ttctatcaat ttcatagaaa ttttgatttc
    15661 gtaaacgaag agacaatctt acatgtcact tctcatttaa tacgccacta ctagacaagc
    15721 aaaatcatta ttacagtagt tccagttctt caattaacag tcacttacaa tcaaattgag
    15781 tttgaactag ctgaagcgac cacagaccta tttcttagtt atattcgcta aaaaagtccc
    15841 cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat atcgaaaaat tatttttaga
    15901 atagtcccca aaagcctgaa atagagctaa aaaactccac ctgattgggt ggagttaagg
    15961 gagattatta tgaaaaagaa aagtttagga ttttattaaa taaagttagg aggtctttat
    16021 ttaatgacta tataatacta gaccatcctt aaattttgct taagaaaaaa caagttttgt
    16081 tattttttac gattcatcca agtcatccct atacaattat aggtggataa gatttcaaag
    16141 cccatagaac gatagaagcc caaggttctt tctgtctctt ctgtcaccag ctggacttga
    16201 taggcatctt tgtaatcctc taaagcctct ttcatcaagg cactaccaat tccttgacgc
    16261 tgatagattg gtaaaacgat taaatcctga accaatactg atgagaatcc atctccaacc
    16321 aaacgaatca agcccaccac agcatcacca tcaagtgcca gataaattac taatgaatga
    16381 gataaggcct gctccagcat ctctggttga tgggtataat ttgtccaacc gacagcctga
    16441 tagagatgta aaacatcctc tagcttgaca atttcttgct ttttaatagt aatcatctca
    16501 acacctctta aagttctctc aagctcttgt actgctgtcc atttttatca aaattttcag
    16561 gacgcaacca tgtttccaaa cgatctttga cttgaggcca gtccttatca atcatagaca
    16621 accaatccgt atctctcgta cgtcccttat aaaccactgc ctgacggaag gttccttcat
    16681 aaataaagct caaacgctcc gcagctcgtc tggatggcag atttaaggca tcgcatttcc
    16741 actcgtagcg acgatagttt agctcctcaa aaacatagcg agctaggaga tactgagctt
    16801 cagttcctat ccgtgtaccc cttagctctg gagaaaaagt gactgctccg acttctatta
    16861 ctcggttatt ctggtcaatg cgcatgagag aaaaagatcc caaagcctta ccagtcgcct
    16921 tgtctatgat tgcatagtaa aaacggtcct tacgagccaa catctgatgt aaaaggctaa
    16981 ccagctcccc catatctgct actggctcct gaaaaaggta ggtccacatc tcctgaggcg
    17041 tatcaggccc ataaacagct agcaaatcct ccgcatgctt ttccactgaa agagcctcta
    17101 tcctagcata acgcccttct aagaaatcaa tagaaggcag ttcacctggt gtataacctt
    17161 ccattgactc cccaatcatt tgaccatatt catttactgg catagctttc tccttttttc
    17221 acttttcaaa ctttatagtt gcccatagta taccatactt tcataagaag attcaaaaaa
    17281 tcctccagat tctactctga aggattccta ccttatttca caacgatatt aacgagttta
    17341 gttggctcaa acagtttggg aaactgtttg aggttggaaa tcagcaaata aattggttat
    17401 tttacaacaa tattaaccaa tttatttggt acactaatca ctttcacgat ttccttaccg
    17461 tcaatttctg ctttgacttt ttcatccgct agagcaattt cttgcaattc ttcacgtgat
    17521 aggtctttag cgaccatgag tttggcacgg actttacctt tgatttggac aacgatttcg
    17581 atttcgtctt caaccagttt actttcgtcc catgttggcc aagtcacgta agagattgac
    17641 tcacctgttg cagcaacagt ttgccagagt tcttctgcca agtgaggtgc aaatggggca
    17701 atcaattgga taaatccttt ggcatagtca acatagagct tgtcttcctt gttagccgca
    17761 ttgacaaaga ccataagttg ggcaatggct gtgttgaatt tgagggaatc aatttgctct
    17821 gtgacagctt taacagtttc gttgtaaacc ttgtcaagag cgccattgtt ttccgcaaca
    17881 atttccttac ttctaatcaa acggtaaaca cggtcaagga acttacgact tccttccaga
    17941 ccttcttctg accaagcaat cgaagcatcg agtggtccca tgaacatttc atagacacga
    18001 agggtatcag caccgtattg ttccaccaca tcgtctgggt tgacaacgtt cttgagcgat
    18061 ttagacatct tggctggcgc ttgctccaac tcttcccctg tttccacatg gaagaaagaa
    18121 ccatcacgtt tttcaacctt gtcagtcgcc acaagagccc cacggtggtc acgatagctt
    18181 gttcccaaaa tcattccttg gttaaagagt ttttggaatg gttccttagt tggaacaaca
    18241 ccgaggtcat agaggaattt atgccagaaa cgagcgtaaa gcaagtgaag tacagcatgt
    18301 tccgcaccac ccacgtagat atctactggc aaccattgtt tgagcagatc ctcgtcagcc
    18361 aatttctcag tattgtgtgg gtcaatatag cggaggtagt accagcttga acctgcccat
    18421 tgtggcatag tatttgtttc acgacgacct ttgacaccat cttcacgagt cacttcaagc
    18481 cagtctgtca agttagctag tggactttca ccagtacctg agggacggat atccttggtt
    18541 acaggcaaaa caagtggcaa ttcactttca gggacagctg ttgaagttcc atcttcccaa
    18601 tgaatgattg ggattggctc accccagtaa cgttgacggc taaagagcca gtcgcggaga
    18661 cggtaggtta ccttctcttg tccacagcct ttttcttcca accaagccac aatcttggca
    18721 atagcgtctt ctttgttgag tccatctagg aagtctgaat taacgtgaag gccatcttct
    18781 gtgtaggcag cttcttcaac atttccacct tcaagtactt cgacgattgg aaggtcaaat
    18841 tgtttggcaa attcccagtc acgttgatcg tgggcaggca cagccattac cgcacctgta
    18901 ccatagctag caagaacgta atccgcaatc cagattggaa tttccttacc attgacagga
    18961 ttgatggcat aagcaccagt ccatacacca gttttttctt tggcaaggtc ggtacgagcc
    19021 aagtctgact taaggctggc ttggtgtttg tagtctgcta ctgcttctgc ttgctctgtg
    19081 cttgtgatgg catctactaa ttcatgttca ggagccaaaa cagtgaaagt cgcaccgaaa
    19141 agtgtgtccg gacgagtagt aaagactgta aattccttgt ctgttccttt tactttgaaa
    19201 gttacattgg caccagttga tttaccaatc cagttgcgtt gcatatcctt gatagactct
    19261 gaccaatcta gttcatctaa gtcattgagc aagcgctctg cgtaagccgt gattttgagc
    19321 atccattggc gcattggttt gcggacaact ggatagcctc cacgttcaga agttccgtca
    19381 ggaagcactt cttcattggc aatggcagtt cccaattcct caacccagtt tactggcact
    19441 tcagcttcat aggccaagcc tttttcgtaa agcttggtga aaatccattg agtccacttg
    19501 tagtagtttg gatctgttgt gttgacttca cgatcccagt cataagaaaa tccaagcgca
    19561 ttgatttgac gtttgaagtt ggcaatgttt tccgctgtaa attctgctgg gtcattacca
    19621 gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat cccaacccat tgggtgaagg
    19681 acattgtagc cttgcgcacg tttgtaacga ctgaggatat cggttgcagt ataaccttct
    19741 gggtgtccta cgtgcagacc agctccagac ggataaggga acatatcaag cgcataaaac
    19801 ttaggttttg atgcatctgt tcctgtctta aatgtatgat gttctgccca gtagccctgc
    19861 cacttaggct caatttcttt atgattgtaa aaactcatgg tctctctcca attttgtgat
    19921 gttactatta taccattttt gagggaattt gaaatgtgtt taaaggaaaa actgtatact
    19981 gtatctcatt aattttgatg actatgaatt gctctatctc ccacttttta tcccattgtt
    20041 atgcagtaaa tcaaggagta agtcaagaaa cttatctcgt atagcacaaa atacttattt
    20101 tagaccgaag ataaaactgg aatgctaatt aatgtattca caataaatag ccgacaacct
    20161 atgtcgcttt ttgatttatg aagaacgtat agtggactga atctgaaata gtacgaaaca
    20221 attgctaaaa tacttataga aattaatttt actttcccaa tcgatttgtt ctcatcttat
    20281 ttcaatctgc tatagaattc aatacgagga tcaataaaag actaccattt ggaaaacagt
    20341 agtccttatt gtttattttg aatttacata ggcatctaca gcgataatag cttgagcaac
    20401 atctgaaggc gaaatcttaa acggcatctg atgaattgtc tcaccctcca tagttgcttg
    20461 tttaccaact tttattaaat catcatatcc aacttgatcc aaatgcattt cttttagagt
    20521 tgttggcata ccaatttttt tgtaaaactc aatatactta tcaagttctt ctttaggtct
    20581 attttccaat aatagttgta ctaaagttcc ataagctact ttttcaccat gtgttaaatg
    20641 atgaatgtca cctgtcaatg cagtaaaacc attatgaatt gcatgcgccg cagctaatcc
    20701 tccactttca aaacctaggc cactcaataa agtattagct tcaacaatat tttctaatgc
    20761 tggtgtcacc actttagctt cacaagctgc catagcctgt aaaccatctg caaacagcgt
    20821 ttcttcacat ttcttcgcaa ttgcaactcc agccaatgtt tgctgttgtc ccaacatagt
    20881 ttttccattt gcctgcataa ccgcacgcgc ctcaacccaa gttgctaaac catctgcaat
    20941 accagacgct aataaacgct taggggcttg tgaaataact tttgtatcaa ccaaaactaa
    21001 atctggattt ttagaataaa atagataatg atcaaatgca ccttcatctg tataaataac
    21061 agataaagca gatacaggtg cgtcggtcga tgcaattgtt ggagcaataa taacaggctt
    21121 ttcaatcaaa tctgcaatag cttttgcgct atcaatcgtc tttcccccac caagaccgat
    21181 aatactatca caattttctt tctcagccaa ggcaacaact cgattgattt cattgtcaga
    21241 agcttcacca ttaaatagcg ccagaacaat atggaaacca tacctatgta gataatcttc
    21301 aaatcgtttt ccaacaatat cataaaccaa ctgatcgcat aatagaatag ggtaatttcc
    21361 caaatccaaa attgatttgg cattttcaaa caaggcattt tccccctgaa tatatctaga
    21421 aggactagca aaaattctca tatgtatttc ctctctatct aaatggaacg actattttga
    21481 gtaacaaacc aatcgtcaga aaaatcatca accgcctttt ggatagatgg catggcgaaa
    21541 gctgattcaa aaacatctgc tcctgctgta acagcatgcg cacctgcagc taaagcatta
    21601 tttacttgtg ctacattttt aaaggatgca gctaaaatct tactaggaga gttctgtcta
    21661 tcaatagcaa gagctaattg acgaatgaca gaatttgaat caatgttcag attttccatt
    21721 ctattataat atggagctag gtaatccgct cctacttcga tagctaataa tccctgaata
    21781 actgtataaa tagctgttgc agtgatatgg tagccctctt tttttagcac ctttattgca
    21841 cgtaatccag ctggagtaac aggtactttg ataaatatat catctcctgc ttgtcttcga
    21901 atttcatgag catcctttaa gatgccttca aaatcttgag aaatcacctg aacatgaata
    21961 gagggtgtag agccaatcaa ttctcttaca tctttgattc gttcaaaaaa attaatagaa
    22021 ccctctcttt ttgcaatagt gggatttgaa gttaccccag ctagcggcaa aatttcagac
    22081 cactttttaa tctcatctaa atttaatgtg tcaagcataa attccataaa aagaacctct
    22141 ttattttaca aagtatgctc agtacgtcct ataatgtcat cttgggttgc cttagaaaga
    22201 acattgaaga atgcagagta tcctgcaaca cgaacaatta agtctctgtg tttttcagga
    22261 tgtttctgag cgtcaatcaa cgtctctctg gaaacaacat tgtattgaat atggtaccca
    22321 tgtaaacgat taaagaatgt tcgtaacaaa gcaattagtt ttaatttatc ttcttctttg
    22381 gctaacgttt gaggatttac tttctgattt aagagaaccc cacctacgat ttcatctgtt
    22441 ggtaattttg aaacagattt taaaacagat gtagggccgt gttgatccat attatgtgat
    22501 ggtgaacaac cctctgctaa cggtgtaccc gcgttgcgtc catctggagt tgctaatgtt
    22561 ccacgtccct gccctacgtt ggctgagata gaagatgttc ctgaataacg aattcctcca
    22621 ataggccctc ttccataacg tgtattagga tatttagcaa tttcatcaac ataaatgtca
    22681 taagcagcag taaccaattt gtcagcataa tcatcatcat taccatactt aggtgcatca
    22741 tgaatcaaca tttcttgaat gaccttacct tcttctccgg cataatctgt ttccagtgca
    22801 tgccaaagct gacttgggct tatacgttct tcctcaaaca ccaatttttt aattgcagct
    22861 aatgaatccg acaaatttgc aattccaact tgcaatcctg atatataatc atatactgct
    22921 ccaccttctt taaggtgttt tccacgacca atacaatcat cagtcaatgc tgaacataga
    22981 atatcaggaa cttctcgttc caatgataaa tcaatagaat tttcaacaat aacactcatt
    23041 cgtgtcaaat atcttagtgt tttatcccaa gcattttcta attcagaaaa gttcttcata
    23101 tccttaaaat gaccaaagct tggtgcaaac cgtttacccg aagccggatc aattccatca
    23161 ttcatcgtga taagtagaac cttagggaag ttcatataac tcatacctgt gcaacgatag
    23221 ccccatttcc ctggaactgc cgtttcaaca catccaatgg cactgtaatc ataagcatca
    23281 tcttccaata ctccttttgc aataaaagaa ggaataataa tctcatcatt attaaatgca
    23341 ggcataccaa aaccaagttt catcacttca atacactcat tcatgaaacg agcatctaaa
    23401 cctgcatggt aacgtacagt tagattaggt tgcggtagat gggtttgtgc aactgatttt
    23461 aataccaaat aagataatgg gttaacagca tccttcttat ctcgagtctg tccaccaatt
    23521 gtaacatttt gatataaagg acttcctgct gaagaaaatg tatgtgattg actgcgaacc
    23581 ttattgattg taattgtctt aatccaaaga tttgtcagac gttcaacaat gctatcttct
    23641 gtttctttac cactttctaa atcagccttc atatatggat acatatattg atcaaaacgg
    23701 ccatatgaaa gagagtggcc attagattca atttgtaaaa tacattgaat aaaccaaact
    23761 gattgaatag cttctgcaaa agtagttgcc ggttcatatg ggactctaga gcaaatatct
    23821 gcaatctcaa gtaattcttt cttacgttta ggatttgcat tttcggctaa acttttagca
    23881 agagcaacaa agcgctttgc atatacttta atagcatcga ttacgataaa tatagagtcg
    23941 taaaaatgat atttatcaat acttgctgga tctgttaaat ctagagctac ttttgcttta
    24001 cgagcccgct cttcaaaacc tcttaaacca aattgcaaaa gtttctgata gttaactgct
    24061 aggtgagcat ctccagaatt catcttacct tccataccga agaatcctgt ttccatataa
    24121 acagacactt cttcaggtaa taaggcacca gctctagcac gtaaattatt attttcccaa
    24181 aacggagcaa tacttctaag ttgttctttt gtttcttctg taatatagaa aacatctcca
    24241 tcacgctttt caaaaagatc caactcattg agaacaaatt ctagcgtata ttccggaaaa
    24301 ataggagcat ctttattgga agaagcttga tttcccgcaa tcatagattc ttcttcaata
    24361 tagatagtca tattttccaa aatttctttc agcatatatg cacgttttag gacattaggt
    24421 tgttccttgt atcgttcata ggcgcgtgtt gctagaactg ctctctcagc atcaatataa
    24481 ggttttttat ttaaaacatc ttctcgatat ttgttcatcc tttcagtaag gctgccaaaa
    24541 tattctgtct tgattgttgt tcttgctact tctgtattta ccatcgtaac cacctttctt
    24601 tcgaaaatat ttttatttct ttcgaaaata taataccgat ttttatagaa attttcaagc
    24661 ttttaactag aaaaatttta actctctaat ttttagcaca aatgtgtgag tttaacagta
    24721 acacacaaat acctctaaga ttgtttgatt tcattttggt aagctaaacg atcctgtact
    24781 ttaaagaatg gaaaataaac caatgtagac atcgctaata tcaccagctg agtaataact
    24841 ccttgccatc cacccaccaa aaatcctgat aaaatagctg gtgtactcca aggcaatgtt
    24901 acccctgaga atggctgcat gaaacctgtt gcaatagctc catatactat cacagctgca
    24961 agtacaggaa caagaatgaa aggtacaaac ataactggat tcatgacaat cggaaatcca
    25021 aatacaactg gctcatttac gttaaatatt gctggaaaag ctgcaacttt tcctaaggtt
    25081 tggtattgtt ttgattttgc tgcaaaaagc atggcaacta caagaccaaa cgtaatccct
    25141 gaacctgata gaattaaaaa tgaatctaaa aattgttgag taacaatatg tgcaccattt
    25201 tctaatgata gattagcaga ggctaacata gctttattag cgtcaagatt agataaaagc
    25261 agagctgtca ctactccatt tactaccgat tgcccatgaa caccaaacca ccacaaaaat
    25321 gatataaaga atgcaattcc aatagcacca tacaaagatc cagttaaacc ttgcaacgga
    25381 acttgaatag cagaataaat catttctatg aatgttccgc cattagtcaa tgacttcgct
    25441 aaaatatata caatcataga agataagaaa attacaaatg ctggaatcat tgcttcaaac
    25501 tgtttggcaa tagcttgtgg aacttgttct ggcatcttaa taacaatttt tctctttata
    25561 aagaaggtat aaatacttcc tactaccaaa cctataatga tagcaccgat aattccttgg
    25621 cctccaaacc aaactttact aatagcgtcc ccaatcgcct caccttgttt agggatataa
    25681 gatgatctta gcaaaataaa gaatgcagat acagatagaa ctccagctgg taaagcctct
    25741 actccgctat tcttagcata agaataggca attgaaaaac aagaaattag acccataata
    25801 gcaaaagttc ctgaatatac ttgcataaac ggctctgtcc aattagctcc aaaaacacta
    25861 gcaatgctct tatttaatcc ttcgaacggc aattgtccca taatcaagaa caaactacca
    25921 actactgtca atggcaaaat tgctaacatc ccatctttta gagctataat gccacgcata
    25981 ttcacaaact tcatcatcgg tgcaatgatt ttctgaacat ccatctttga cataataaat
    26041 ctccttttct tacccactaa tcaaagatag ggccaaatct aatacttttt tcccatctaa
    26101 cataccatag tccatcatcg gaataacagc tatcggaaca tcacacttat cacaaatttc
    26161 ttttgattta tctaatgtat aagcaacttg tggacccaat agtgcaacat ctatatttgg
    26221 cgcataatcc gctaatttag actgagaaaa cgcctctatt tctgcctcaa ctccactagc
    26281 ttgcgctgca attttcatat tatttacaag cataccagta gaaaaacctg ctgcacaaaa
    26341 caaaccaatc ttcaccatta tgttttcctc ctctatgtta ataacaatga taatactcta
    26401 gtattatttt ttatgaagtt cttttctcaa actaataatt tctttgatta aattaatctc
    26461 cgtcatacta gtcatgagat gatcttgtga atgaacaaag agagctgtaa tttctgtctt
    26521 tgtaccagac gcttcctgtg ccaagaattt tgtttgtaga ttatgagctt ctaataaggc
    26581 ggcatcagct aactggattt cttcttcaca tcgttcacat gtaccgttct ttatgtaatc
    26641 taatgcttta taaatatgtt gtttcgcatc gccagcatat aaaattaaac ccatgataat
    26701 ttgatctgga acaataattt ccataaattc ctcctcattt cactatgtaa aaagcttttt
    26761 tcatttgaaa ttttaaaatt ttcttttgta actatagaat acaactacat aataaagttg
    26821 tcaatccaaa aacaacagat aaactatttt tgtttatata agcacaaatg tgcaatcgac
    26881 taaaaagaaa aaacgataaa ctgatcagtc tatcattttc actcacctag aatctctcta
    26941 aaagtcaaat caccatcttc ttccaaaact tttaaaattg tatttttatc tgtaatcaaa
    27001 tgatttacta aattagcacg caagacagaa agaatcgaag atactttcgt atcaccgtaa
    27061 gcaacagcta aactttgagg aatatttttt aaatcttcca aagagatggc tatcgttctt
    27121 tcctgtaagt tttcatagac ttctttacct tttgaatcaa aaaatcgaca acaaatttct
    27181 cctacagttt taactttggt caattcttta aagtcatcct ctgtaagcat gtctaaccat
    27241 tgatgtttcc ctttattgct aaaatcacca attccgacta cagctatatc taaatctttc
    27301 caactatttt tcaaattttc aaaatatctt gattgcaaaa taccatctgc taacaattta
    27361 ttttcttgca caatcgttgc attcataaat gtacactctc catgaaattt tctagacatt
    27421 tcataaatca gtgtattcac atggtattta gcgtgtatgt gactaggacc acctgctaga
    27481 ggatagaagt gaacatttcg gacactttta ctgtgaatta aatctactaa attacttaaa
    27541 cttttccccc aagaaaagcc aattttcata ttatcatcaa ttagattcct aaggacgcct
    27601 gctgcaactt gagaaattct ttcagataaa attgttggag tatcatcaaa ttcatttgga
    27661 ataatttcta aactttccaa actgtatttt tcttttacat aattttccaa cttaaacata
    27721 ttggtatcaa aattctctat ttcaatttta acaattccta cattccttgc ttctgttaac
    27781 attctactaa tagaggttct ataaattcct aattttgctg ctatttgtga ctgatttaag
    27841 ttttcaatat aatacagata agcaatttta gaaagcagtt tattcctatc ttgattcata
    27901 cacttaacct cttacgaaac taccttaacc attatcccag cattttctaa tgtagctata
    27961 ttttgtttag aaagtttttc gtctgttatt acttcataga cttgacttaa agcaaatctt
    28021 cttactgtac ctcttttatc aaatttactt gagtcagtta ggacaatgac tttatccgac
    28081 actgctgaaa tatattgaac tacctcactg cgcattaaat cttttccggt aaagcccatc
    28141 tctttatcgt aaccatctgt cccaacaaaa gcttgacaca catgaaaagt ctgtatcatt
    28201 tcttttaata aaggtcctac agtcacctgt gaatctttct gaaactcacc accaagaaca
    28261 ataacacgac atgaatcata agctctcaca aaatttgcta taaaaaacga atttgttaca
    28321 atcgtaacat ttcttttttg cttgcaaatt tcctcagcaa gtaaagcaca ggtcgatcca
    28381 gattctatca ttattgtttc attatctgac accaatttta ctgcttcctg aacaattttt
    28441 ctcttagttt cataattaat tgacaaacgt acatttaagt catctccact atttaataca
    28501 gcatatccat gctctctgtg taataaacct tttgactcta atttatctaa atcttttcta
    28561 atcgttactt tcgatacatt taatttttcc gataatgtat taacgtcgat cttttcatat
    28621 tctgatacta atttaataat ttgttccaat cttttcattt tacacctccg ttttattcta
    28681 ccaaaataaa aagcaaaaaa caacaaatta aactttcgtt cgtaattgtt tttctttcgt
    28741 ttttgtgata ggatagactt atgaagagga ggaactctta tggaaatatc taaaggaatt
    28801 atttttaata ttcaacactt ttcaattcat gacggcccgg gtattcgtac aactgttttt
    28861 ttaaaaggat gtcctctgcg ctgtccatgg tgttctaacc ctgaatctca aagaatgaaa
    28921 cctgaaaaaa tgaaagatgc tcaacgagag aaattcacct tagtcggtga agaaaagact
    28981 gtagaagaaa ttattacaga ggtattaaaa gacaaagaat tttacgaaga atccggtgga
    29041 ggtttaactt tatcaggagg tgaaatattt gctcagtttg aatttgctaa agccatctta
    29101 aaatcagcta aagaacatca catacacact gccattgaaa ctactgcctt tgttgatcat
    29161 gaaaaattta tcgatttaat tcaatatgtg gattttatct acacagacct aaaacattat
    29221 aattctataa aacataaaaa agtgactggg gtttttaatc aaatgattat taaaaacatt
    29281 cattatgctt tttctcaaaa taaaactatc gttttaagaa tcccagttat tcctaatttt
    29341 aacaatagtt tagaggatgc agaaaaattc gctactctat ttaactcatt aaatatcgac
    29401 caagttcaac tactcccttt tcatcaattt ggtgaaaaca aatatcgttt attaaatcgg
    29461 aaatatgaaa tggatggagt caacgcactt catcctgaag atcttattga ttatcaaaag
    29521 gtatttctga accaccatat taattgttat ttctagttca tttccttgaa acgctctagc
    29581 tatttgcaga taacaagcat ctatataagg ttatcaaaga agacttcccc atgactctaa
    29641 atgaatatat cttacaatat cgcctgaaac aagctataga taagatggct gaatctccca
    29701 actcctcttt aagcgatatc tctgaccaag ttggattttc agactataaa tattttgcca
    29761 aagtatttaa aaagtatctc catatttccc caaaaaaatt gaaatcactc ggaagaatag
    29821 taaaataggc tattcttctt tttgtagaga attttactct aatatagtga attgaaataa
    29881 gatgtgaaca tctctatcag gaaagtcaaa ttaatttata gaaatatttt agcagtcaag
    29941 gtgtactatt atagattcaa tgcactatag aatagaattg caggttttaa aaacgcttac
    30001 attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga
    30061 tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag
    30121 acggtggttc gctagtcgtt tattgaccaa actcagaagg cttaattgga gcaactattc
    30181 ctgcctttga agaaaaatat ggtatcaaaa tagaactgat tcaagctggt actggagaac
    30241 ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt
    30301 cttatacaca atatgctacc cacggagaac tctttgaaaa ctatatttca aaagaaaatg
    30361 ataatgttat caaagaatat caaaacacaa ctggcttctc tactccttat acactagatg
    30421 gtagtgtttt aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa ggatatagcg
    30481 accttctcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta
    30541 gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg
    30601 cttggtctta tgtaaaagat cttttcacac ttattgatgg tatagtaaaa tgaaataaga
    30661 acaggacaag ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt
    30721 gtactattct agtttcaata tactataaaa tcggtttaag ttcatctggt gtctataaag
    30781 cagtcgctga tggagaaatg actgttggtc tctcttatga agatccagca gttaaactct
    30841 taaatgacgg agctaacatt aaggtagtct atccaaaaga aggaaccgtc ttcctacctg
    30901 ctagtgctgc tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa tttatcgatt
    30961 ttattatctc tcaagaagta caagatacac ttggtacaac cactactaac cgtcctgttc
    31021 gtaaaaatgc taaaacaagc gaaaacatga aaccaattga caaaatcaaa acactcactg
    31081 aagattatga ttatgtcatc aagaataaat cagatatcgt taagaaatac aacgaagtct
    31141 ttacagatat ccaatctaaa cagtaaaaga ggttcactat gagtgagatc aaaattatta
    31201 acgccaaaaa aatctaccac gatgtccctg ttattgagaa tttgaacatt acaattccaa
    31261 aagaaagtct ctttaccctt cttggacctt caggatgtgg gaaaacaacc cttcttcgta
    31321 tgattgcagg tttcaacagt atcgaaggtg gagaatttta cttcgatgat acaaaaatca
    31381 ataatatgga acccagcaaa cgcaatatcg ggatggtttt ccaaaactac gctattttcc
    31441 cacatttgac tgtccgagac aacgttgctt ttggtcttat gcaaaagaag gttccaaaag
    31501 aagaattgat tcaacagacc aacaagtatc ttgaactcat gcaaattgct caatatgcgg
    31561 atcgaaagcc cgataaactc agtggtggac aacaacaacg tgtcaccttg gcatgcgcct
    31621 tagcggttaa tccaagtgtt ctcctcatgg acgagccact tagtaatctg gaggccaaac
    31681 ttcgcttgga tatacgtcaa gccatccgag aaatccaaca cgaagtggga attacaactg
    31741 tttatgtaac ccacgaccaa gaagaagcca tggctatttc agaccaaatt gctgttatga
    31801 aagatggggt gatccaacaa atcggccgac caaaagaact ctatcataaa ccagctaatg
    31861 agtttgtggc aacctttatc ggacgcacaa atattatccc tgccaatctt gaaaaacgga
    31921 gcgacggcgc ttatatcgtc ttttcagatg gctatgccct tcgaatgcta gctcttgatc
    31981 aggttgagga gcaagctatt catgtaagca ttcgtcccga agagtttatc aaagatgaat
    32041 ctggagatat tgaaggaact attagcgata gcgtctatct tggactaaat acggagtatt
    32101 tcattgagac aggttttgcc tcaaaaattc aagttagtga agaatcaact tttgaagaag
    32161 aactacaaaa aggcaatcgt attcgtctac gaatcaatac gcaaaaatta aacatctttt
    32221 ctgcagatgg ttcccaaaac ctgataaaag gagtcaacca tggaacgtaa aaaactaaat
    32281 atttggacag cctcctcttt cttcatcttt cttacctatc ttgtctttct cgtttatcct
    32341 atcgttaccg tgctcaagta agcacttata catgaaggat aattctcact agctaatttt
    32401 gtcactttct ttagtaaaac ctactactct gagacactag tcaacagttt cagggtttcc
    32461 attaccgcta ctgtcacttc cttagttgta ggaaccctat tagcttatct cttctctatg
    32521 tatgacttca aggggaagaa atttctacaa atattgatta tcattgcttc catgtcagct
    32581 cctttcgtag gagcctactc ctggattctc ttgctgagac gaaatgaggt catcactaaa
    32641 tttttgacaa atgcccttca tcttccagct atcgatattt atggattcaa aggaattata
    32701 cttgtcttta cactgcaact attcccactg gtatttctat atgttgctgg aacaatgaac
    32761 agtattgaca attctctact tgaagctgct gaaagcatgg gatccttcgg atttaaacct
    32821 atcgtaacgg ttgttttacc tctcctagtt ccaaccttac tagcagcccc ttgcttgtat
    32881 ttatgagagc attctcagac tttggaacgc ctatgttgat tggcgaagga tatcggactt
    32941 tccctgtcct gatttatacc caatttatta gcgaggttgg aggaaattct gcttttgcaa
    33001 ttatggcgat tatcattgcc ttggcaattt tccttatcca aaaacacatt gcaaaccgct
    33061 acagtttcag catgaatccg ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa
    33121 tggctgccat ttatgcaaca gtctacggaa ttatctttat ctctgtttta cctcaaatct
    33181 acttaattta tacctctttc ctaaaaacat caggtatggt atttgttaaa ggttattctc
    33241 caaacagtta caaggtagct ttcaatcgta tgggatctgc tattttcaat accattcgta
    33301 tccctttgat tgccttagtt ctagttgttc tatttgcgac atttatctcc tacctagccg
    33361 ttagaaaacg gaatttattt acaaacttaa ttgacagcct cagtatggta ccttatattg
    33421 taccaggaac cgttctaggg attgccttca tttcttcctt caatactggt ctatttggaa
    33481 gtggatttct tatgattaca gggactgctt tcatcttgat tatgtctcta tctgtcagaa
    33541 gattaccgta tactattcgc tcatctgttg ctagcttaca acaaatagca ccaagtattg
    33601 aagaagctgc tgaaagctta ggaagtagtc gtctcaatac ctttgctaag attacaactc
    33661 caatgatgct atcgggtatt atttctggag ccatcctatc ttgggtcaca ctgatttcag
    33721 aactctctac ttctatcctc tatcctcctc tacaatgtca aaacaagaac aatgactgta
    33781 gctatttata cagaggttct cagaggaaat tacggtgtag ccgcagcctt gtcaactatc
    33841 ctgactgttc taacagtagg ttccttgctc ttgtttatga aaatctctaa aagcaatagc
    33901 attacacttt agttttccac atcaaaaaca gccgaaagga gtaccctcgg ctgttttttc
    33961 ttatcttgat attcttatca aatccctagc tcatggcaag caagtctaga ctgattaaat
    34021 agagccttct tccatcttct cacgtcctag ctctcggtaa ctacggtcac cgacaacttc
    34081 aaccctaaac tgaccgtcct catattcaag aatcgtcacg ctaccattat ctagaccatg
    34141 cggatgcatg ccattaatca gataaacaat ggttccaata gtcattccat ggctgacaac
    34201 aagggcgtta cctccacctt gatcttccat ttcttttgca attatttcaa agccttcctt
    34261 gattcggcca ctgagttttt cccaaccttc agcccaacca gctgtatcga cctctaccaa
    34321 gccctcagcc agttcagcat aagacaattg gtgaacgtgg tccacattaa agatacgagg
    34381 aataatgccc atgaaaagat cgccatcata ggctccatca aaactaccga aacaccattc
    34441 tctgatacgc ttgtccatgc gataagggat tttcccctgc aagccaagtt cttcaagtat
    34501 aattcccatg gtctgaatgg tacgaccaga atcactcgaa taagcgcgct caaactgtag
    34561 atcagattct cgcaaaccga ttcctaactc ttgaatccct cgttcacctt cagcagttaa
    34621 gggagtatcg ctccaacctt gcgcgcgacc aatcgtgtta aacatggtct tgccatgacg
    34681 taccaaatac aatcgtactt ttaccatttt ccgtcctccg tttgcttcta ttatatcatg
    34741 aatgataaaa taaaagccac ccatacaggc gacttttgaa ggagattatt atgaaaaagt
    34801 ttaggagttc attaaataaa gatattagat gaaaatcaaa ttcaaactaa ttcagtgtta
    34861 tctgtttcaa ataatattag gaggtcttta tttaatgatt atagtataca catctaatct
    34921 taaatagaac ttaaaatttc tcctattttc ttaattttta aaactatgaa ttggtgctgg
    34981 gatttgtcca ccgcgagaga tgaagtcgac agacgaagcc ccattaacct tcataacggg
    35041 tgcagttcct aatagaccac caaactcaat catatcgcct tctcttcctt tgggaatgat
    35101 acgaacagct gttgttttca tgttgataac accgattgct gcttcatccg caatcatagc
    35161 cgcaatagtt tcagcaggcg tatcttctgg gatggcaatc atatccaatc caacagaaca
    35221 gatagccgtc atagcttcta gtttttctaa attaagagag ccattttgca ctgcagcaat
    35281 cattccttca tcctcagaaa cagggataaa ggcaccagat agaccaccga cctggttaca
    35341 ggccatcact ccaccctttt taacttggtc gttcaagagg gccaaggcag ctgtcgttcc
    35401 atgcgtgcca actgtttcta gccccatttc ctcaaggaca cgtgccacag agtctccaac
    35461 cgcaggggtt ggtgccaaac tcaagtccac aataccaaac tccacaccca gtctctcact
    35521 ggccatttga ccaaccaatt gaccgatacg agtgatttta aaggcagttt tcttaactgt
    35581 ttcggctact acatcaaagc tctgtccacg aactttttcc aaagcacgtt tcacaacacc
    35641 aggaccagaa actccgacat tgatgataac atctgcttcc ccaacaccat gaaaggcacc
    35701 cgccataaat ggattgtcct caacagcatt agcgaataca accaacttgg ccactcccat
    35761 atctgaaaga tttgctgttt ccttgataat tcgtcccata tctgccacag ccgtcatatt
    35821 aataccagac ttggttgagc cgatattgac tgacgagcag accttatccg tctcagccaa
    35881 agcgcgagga atggaattga tgagaatctc atctcccttt tgataacctt tttgtactaa
    35941 ggcagaaaaa ccaccaataa agtccacacc aatctctttc gcagccttat caagcgcttt
    36001 tgccagaacc acgtagtccg tcgcatctgt cgctgcccca atcagagaaa taggtgtcac
    36061 cgatacacgc ttattaacga taggaattcc caactcagcc gcaatttcat caccaacagc
    36121 tactaaatta gccgcctttg tcgtaatttt ttgatagatt ttctccgcag cacaattgat
    36181 atctggatcg atacagtcca aaagagaaat ccccatggta atggttctaa tatcgaagtt
    36241 ttgctcctca atcatggcga tggtttcagt aacttgtcta atatccatga tgacctccta
    36301 gatattatac atagcttcga aaatcgctgc actctgaata ttgattttta cattcaaagt
    36361 ttgcccaaaa gcttcaaatt cattacgaag ataggtaaaa tcttgctttt catcactaga
    36421 tacaacagcc atcatcgtaa aatattcatc taagacagtt tgagagatat cgtcaatatt
    36481 caatcctaat tctgcaattt taccagaaac acctgcaaca attccagatt tatctttacc
    36541 aacaacagtt ataatggctt tcataagtaa actccaattc ttcttaaaat aagaaacctt
    36601 aacattaaac aggattcttt tcaaatagta tagcataatt ctaacgaaaa tactcaaaaa
    36661 cgcctgctta cgaagttgtt cttaagaaaa aaacaatttc gtaaacaagc gtctactatc
    36721 ccaacttatg atgagtgttc ccgctaggac cgaaatccta gcggtagacc agagctagac
    36781 taagagcaca agactccatc atcataacac tcaacaaaat tgatgatttt atactaattc
    36841 gatgatcgcc attggcgctg catcaccacg acgtggttca gttttaagga tacgagtgta
    36901 tccaccgtta cgttcagcat aacgaggtgc gatttctgag aacaattttt gaagtgctgt
    36961 agtagaagtg tacttatcag ttgcttcatc atagttttca gatgcgattt cattacgtac
    37021 gaaagcagct gcttgacgac gtgcatgcaa atcaccacgt ttacctagag taatcatttt
    37081 ttcaacagtt ttacggattt ctttagcacg agcttcagtt gtcacgattg attcgttgat
    37141 caaaaggtca gttgtcaaat cgcgaagcat tgctttacgt tgtgagctag tgcgtcctag
    37201 tttacggtaa gccatgtatt cctcctttat ttatctttta atccaagacc caaatcaatg
    37261 agtttgagtt tcacttcttc caaactcttg cgtccaagat ttcgtacttt catcatctct
    37321 gcttcagatt tttctgtcaa atcatgcaca gtattgatac cggcacgttt taaacagttg
    37381 tatgaacgca cagacaagtc cagttcctca atcgtacgat ctaaaatacg gtcgtcagat
    37441 tcagtatcag cttctttcat cacttcagtt gacttagcaa tctcagtaag atttgtaaac
    37501 aaatcaagat gttctgtcaa aatacgtgct gaaagcccta aagcatcttc tggaataatt
    37561 gttccatttg tcaagatttc aagggttaat ttgtcgaaac catcattgct acctacacga
    37621 gcaggttcca cttgatagtt gacttttgta actggtgtat aaatagaatc tacagcaagt
    37681 gttccaactg gtgcattatc ctttttattt tcatcagcag gtacatatcc acgaccactg
    37741 ttaacagtca tagtcgcttt tagagaagaa ccttcaccga ttgtaaagag ataatgatct
    37801 ggatttacaa tttcaatatc gctatctgtc aaaatgtcac cagctgttac ttcagcagga
    37861 ccttcaacat ccagttcgat gattttttcg tcttcaacgt acgatttcac tgcaattcct
    37921 ttaatgttca gaatgatttg catcacgtct tcacgaacac ctggaactgt gtcaaactca
    37981 tgtaacacac catcaatgtt gatagatgtc acagctgctc ctggtagaga agctagaagt
    38041 acacgacgaa gagagttacc aagagttgta ccgtagccac gttcaagtgg ttcgattaca
    38101 aacttgccat aatctttatt ttcatcaatt tttgttatat ttggtttttc aaactcgatc
    38161 atttagttac tccctcttaa acgaaaagca gtgtaatgcg atgattatac acggcgacgt
    38221 tttggaggac gagcaccatt gtgtggcact ggagtcacat cacgaattgc tgttacttca
    38281 agaccagcgg cagcaagcgc acgaatagct gactcacgac cagaacctgg accttttaca
    38341 gtaacttcaa ctgatttaag accgtgttct tgtgcagatt tagcagcagc ttcagaagcc
    38401 atttgagcag cgaatggtgt agatttacga gaacctttga aaccaagagc accagctgat
    38461 gaccaagcaa ttgcattacc atgcacatca gtaatcataa caatagtgtt attaaatgta
    38521 gcgtgaatat gagcaatacc agattcgata ttctttttca cacgacgttt acgtgttggt
    38581 ttagccaaga cttttacctc ctatattatt ttttcttacc agcaatcgca acagctttac
    38641 ctttacgagt gcgggcgttg tttttagtgt tttgtccacg gacaggaagt ccacgacggt
    38701 gacggatacc acggtatgaa ccgatttcca tcaaacgttt gatgttcaag tttacttcac
    38761 gacgaaggtc accttcaact ttgattgcat ccacttcacg acggatagca tcttcttgat
    38821 ctgatgtaag atcacgtaca cgaacatctt ctgagattcc agcagcagcc aaaattttct
    38881 tagatgttgc aagtccgata ccataaacat aagtcaatga gattactacg cgtttgtcat
    38941 ttggaatatc aactccagca atacgagcca tgttttctcc tttctatctt atccttgacg
    39001 ttgtttgtgt tttggatttg ctgggcaaat taccataaca cgaccattac gacgaataac
    39061 tttacagtat tcgcaaattg gtttgaccga tggtcttact ttcatttctt atccctccaa
    39121 gtttttcgat tatttaaagc ggtaagtgat acgtccacgt gtcaagtcat atggactcat
    39181 ttcgacagta acacgatctc ccgctaaaat acgaatatag tttttacgaa ttttaccaga
    39241 aactgttgct aaaatctgat gtccattttc aagttcaacc gtaaacattg cattcggcat
    39301 tgtatcaact actttgcctt caacttcaat cacatcgtct tttgccacgc aaaagcacct
    39361 ccataaattt cgattcgatg cctctagaca cagagacaac aattataagt cagactatct
    39421 cagtataaca tttgtagcgg atttttgcaa gtgcgaaaaa cgctttattt caaatttgtc
    39481 aatacttttt caatatctga gaagacatca ttgatatctt gattaccttc gatgtcatga
    39541 accaaacctt tggcacggta gtgagcaatg attggttctc cttgagcaat attaacatcc
    39601 aaacgacgtt ttactgtctc aggcttatca tcttcacgtt ggtagtaatc ttcttcttta
    39661 tagtcaactg gtgggttaaa gaccttgtgg aaagtttctc cagttacgcg gtggatgata
    39721 cggccactca aacgttccaa gaggctgtca gggttcactt caatattgat aataccttct
    39781 agttcaatgc caagttcagc caatgttttg tccaaggcat gagcttgttc aattgtacgt
    39841 gggtaaccat ccaataagaa tcctgtttct ttaatatcat cttgtgaaag gcgttctttt
    39901 acgattccat ttgtaacttc gtcaggaacc aattcaccct tgtcaatata tgacttagca
    39961 agaacaccca tttcagtttg atttgccatt gcagcgcgga acatatcacc tgttgagata
    40021 tgtgcaacat ggaattgttc tacgattttt gctgcttgag ttcccttacc tgcaccaggt
    40081 aagcccataa tcaaaagatt catgatttga tctccttatt ttatttttaa atagaagcaa
    40141 aaagtctttt cacccctatt taaaaacaaa atagaagagg ggagatccaa ctcttacaaa
    40201 tgtcagagtt taaacctcca ctccctcagt atttactgat tcagtaaata cttttattct
    40261 gttctgtcca tgaaaccaac atacttacgt ttcaataggt aaccttccaa ttgcttgatt
    40321 ccttcgatac ctgtagagat aatgatcaag agacttgttc caccaaaggc aacaacatca
    40381 gaaagaccaa atacatcttt agctgcaatc ggtaaaatgg aaatcacacc aaggaagagg
    40441 gaaccaacag ttgcaagacg acgaagaagt ttagacatat attcttctgt acctttacca
    40501 ggacgaactc catggatata ggcaccactc ttttgtaggc tctctgctgc tttttcagga
    40561 ttaatctgta caaacgtata gaagaatgta aagagaataa tcaacaaagc atacatggca
    40621 ataccagttg gagaagtagt tgccaacatc tcttgtgcta cccttaccca agcccaatca
    40681 tgacctgtgg cactcaaaaa ctgaagaata gccgcaggcg ctgcagtaat cgaactggca
    40741 aagataacag ggataactcc agcagggttt acctttaacg gaaggtaaga gctagatgga
    40801 gcaccttgtg caaccttagt atattggatt ggaattttgt attctgcttg ttgaacataa
    40861 gttgtaaagt aaataatcaa caatacagta ataatcaaaa tgattacgaa aatgatagat
    40921 gaagtgatac ggctacttgg gacgttcaca aagtagtcca catagatgcc ctgaatcatc
    40981 tctggaattg aggaaacaat cccggcaaag ataatcatgg aaacaccgtt tccgtatccc
    41041 ttatctgtaa tttgctcacc caaccaagtg acaatcatac taccagctgt taagatgata
    41101 ccaatcgtca gaaaaacttg tggagttaaa gcagttttaa tcaattgagc tccagccaag
    41161 gtattaaaac cagctgtaat cccgatagat tgcacaaaag cgagaactag agcaatataa
    41221 cgagtagctt gattcaattt tcttcgacct acttcccctt gtttacccca ctctacaaac
    41281 ttgggtaaaa tatccatttg caagagttgg acaacaatag aagcggtgat atagggacta
    41341 actcctaggg caaaaatcga aaagtttttt agggcattcc ccgacaccaa gctcaacatg
    41401 tttaagaagg ataatccact taaagcattc aagctattgg cattcacacc aggaactgta
    41461 atgctagttc cgatacgaaa gaccaaaacg ataaaaattg taaataaaat ttttgatcga
    41521 acctgcttga ctttaagagc ttctcttaat aatttaaaaa acataggtca cctctcttag
    41581 atgacttcta ctgaaccacc tttagcagtg atagcttctt cagctgattt agagaattta
    41641 gctgctttca cagtcaattt cttagtcaac tcaccgttac caagaatttt aatacctgac
    41701 ttttcagctt taacaattcc tgcttcgata agaacaactg gagttacttc agcaccatct
    41761 tcaaagacgt tcaattggtc aaggttcaca attgcgtatt ctttagcgtt gatgttagtg
    41821 aatccacgtt ttggaagacg acggaacaat ggagtttgtc caccttcaaa accaaggcga
    41881 actccgccac cgctacgagc tttttgacct ttttgaccac gaccagatgt tttaccgtta
    41941 cctgatgaag taccacgacc aacgcggtta cgtactttac gagaaccttc tgcaggtttc
    42001 aattcatgaa gtttcatttt tattttctcc tcttttgtaa aatgctagcg ccgataaggg
    42061 agaaaaggtt gtctccccca tcaactcgcc tatacgatgt catcatagat gactatatct
    42121 agttttaggg gatggtacag tgcacatccc ctaaaacttc attagtttac ttcttcaact
    42181 gttactaagt gagatactgc tgtaatcata ccacggatag cagcgttatc ttctttaata
    42241 acagagctgt tcaatttgcc aagtccaagt gctacaacag ttttacgttg tgatggaatg
    42301 cgtccgattg gagacttagt caaagtaatt ttaatttgag ccattttatc ccctttctta
    42361 tgccaaatca gaaactgaaa taccacgaag ggcagcaatt tcttcagcgc gtttcaattg
    42421 tttcaaacct tcaacagttg cacgaacaat gttgattgga gtgttagaac caagtgattt
    42481 agatgtaata tctgccacac ctgccaattc cacaacggca cgaactgcac caccagcggc
    42541 aactccagaa ccttctacag caggtttcaa caatacttta gctccaccga attctgaaag
    42601 aacttcgtgt gggattgttg ttccaaccat aggaacttcg atcaagtttt tcttagcatc
    42661 atctactgct ttacggattg cttctggaac ttcttgagct ttaccagtac caaatcctac
    42721 gcgaccattg tggtcaccaa caacaacaag agctgcgaaa cgaagacgac gtccaccttt
    42781 aacaactttt gtaacacggt tgacagcaac tacgcgttct tctaattcaa ctgcattgtc
    42841 tttaaatgcc attttctagt gtcctcctat tagaatttca atccgttttc acgagctgca
    42901 tcagccaaag ctttcacacg tccgtgatat agatatccac cgcggtcgaa caccacttct
    42961 gaaatacctt tagcgtttgc acgttctgca acgagtttac cgacagcaac ggcttgttca
    43021 gttttagttc cttttgaaac ttctttatca agagttgaag cacttgcgag cgttacaccc
    43081 gctacgtcat caatcacttg agcgtagatg cctgtattag aacggaatac gttcaaacgt
    43141 gggcgatcag cagttccaga gagttttccg cgaacgcgac ggtggcgttt ttggcggagt
    43201 ttgtttttat ctggttttga aatcacagtt ttcacctctt tagttttaaa tcgtgtgcta
    43261 tgcacaaagt tggaaaatag gttggtggtt gatgatcaac cactcaacat tatttacctg
    43321 ttttaccttc tttacggcga acgaattcac caacgtaacg gataccttta cctttatatg
    43381 gttctggtga acgaaggcta cgtacgtaag cagctgtttg accaactact tcttttgaaa
    43441 ttccgctaac aacgattgtt gttgggtttg gaagttcaaa agtaattcct tctggagctt
    43501 caacttcgtc tggatgagat ttaccaacag ccaaaacaag tttagatcct tgaagctgtg
    43561 cacggtaacc aaccccacgc atttcaagtt ctttcttgaa tccttctgat acaccaacaa
    43621 ccatgttgtt caaaagggca cgagtagttc cgtggatagt tttcatttct tttgaatcgt
    43681 ttggacggtg aagagttatt tcagtacctt ccacacggat ttcaatatct tttgagaact
    43741 cacgagtaag ttctccttta ggtcctttta cagttacaac gttgtcattg ttagcgagtt
    43801 caacaccagc aggcaacacg ataactttat taccaatacg tgacatgttt attttctcct
    43861 gttaaattgt caggccagaa cggccagttt tcacggggtt cagatactta tttagttcaa
    43921 gagctgaact gaacacgctc taagaactgt gcaaaataga taagttggct tgtttgtaaa
    43981 caaacttctc caacttccta attttgcttt gttctttacg agctttgtat cttgatttta
    44041 ccaaacgtaa gcgataacct caccaccaac attcttttgg cgtgcttctt tatcagtaag
    44101 caaaccttca gaagttgaaa ggatggcaat tccaagtccg ttaagaactt ttggaaggtc
    44161 ttcacgtttt ttgtagacac gaagtcctgg tttagaaaca cgtttcaagt tagtgataac
    44221 tttctcacca tttggtccgt atttaagaaa tacacggatg acgccttgtt tgtcatcttc
    44281 aatgatttca acgtttttta caaaaccttc gcgtttaagg atttcagcaa tccctttttt
    44341 gatgtttgat gcaggtactt caagtacttc gtgtttagct tggttagcat tacgaatacg
    44401 agttaggaag tctgcgattg ggtcagtcat aaccattttg tttttctcct cttactagta
    44461 gtttgcaagt tgcacttgct agttaataca tgatacaaag agcgtaacag cacgagcaaa
    44521 aatagacaat ttaatgcaga agcaatgctc caaagcaaat tggtcttttt tgccaaagct
    44581 gtagctcgtg ttcaaattgg caagcccaat tgaacccggg ctaaactctg tgtgaaaaag
    44641 ataaactttc ctagaaactt tagtttcttc gtcaagtttc ctatttttac ttggagtttt
    44701 gacgctcttg atatcttaaa ttaccaagat gcttttgtta caccaggaat ttgaccttta
    44761 tgcgcaagtt cgcgaaaagc gatacgactc agaccaaatt tgcggtaaac tgaatgtggg
    44821 cgccccgtaa ctctacaacg attatgtaaa cgagtcggtg aggcgttgcg aggtaattta
    44881 gataaacctt cgtagtcccc tgccgccttt aatgcagcac gtttttcagc ataacggtca
    44941 acaatttttt ggcgtttagc ctctctagct accattgatt ttttagccat tagatttacc
    45001 tcctatatta ttttgcaaaa ggcattccaa ggcctgtaag caatgcacgt gactcttcgt
    45061 cagtgttagc agttgttacg ataacgatgt caagaccacg agttttgtca acgtcatcga
    45121 agttgatttc tgggaagatt aattgttctt tcacaccaag tgtgtagttc ccgcgtccat
    45181 caaatgattt tgttggaaca ccgtggaagt cacgtacacg tggaagtgaa actgatacca
    45241 atttatccaa gaattcgtac atacgttcac cacgaagggt aacttttgca ccgatcgcaa
    45301 caccttcacg aagacggaag ccggcgattg attttttagc tttagtgata agtggttttt
    45361 gacctgagat aagtgccaat tcttcagcag ctttttcaag gcttttagcg tttgatacag
    45421 cttcaccaac acccatgttc aaaacaatct tatctacttt aggcacagcc atcactgatg
    45481 agtagttgaa ttgttctgtc aaagcaggaa ctacttcatt aagatatttt tcttttaaac
    45541 gatttgccat tatacttctc ctttccttcg tgattaatca agcacttcgc ctgatttttt
    45601 gttgtagcga acttttttac cgtctacaaa tttgtaacca acacgaccag ctacaccatt
    45661 tttgtccaaa acttgaacgt ttgatacgtg gatagctgct tctttctcga tgataccacc
    45721 ttgaggaagc tcgttagttg gacgttggtg tttcttaaca atgttaacac cttcaacgat
    45781 aactttgttt acttttggaa gggcagtaag gacaacagct tctgttccct tatctttacc
    45841 agcgattacg cgaactttgt cgcctttttt tacaaacatt aggtttctcc ttgatttttc
    45901 ttacgcccat aagggcaccc tagcttgaag ctaggggact agtttgttcc taaaaattaa
    45961 agtacttctg gagcaagtga cacgatcttc atgaagccac cttcacgcaa ttcacgtgca
    46021 actgggccaa agatacgtgt tccgcgagga gttttgtctt cacggatgat aactgctgcg
    46081 ttttcgtcaa atttgatgta tgaaccatca gcacgacgag cacctgattt agtacgaacg
    46141 ataactgctt taacaacgtc acctttttta accgcaccac caggagtagc ttgttttaca
    46201 gatgccacga taacatcacc gatgtttgca aatttacgtc ctgaaccacc aagaactttg
    46261 atagtcaaga tttcgcgagc accgctgttg tctgcgactt tcaaacgagt ttctgtttga
    46321 atcatttcag ttttctcctt tcaggtttga ttagatgatg accgcttctt caacaacttc
    46381 tacaagacgg aaacgttttg tagctgaaag cgggcgagtt tccatgatac gtacgatatc
    46441 gccttctttg gcaacattgt tttcatcatg agctttgtat tttttagagt agttaatacg
    46501 tttaccatag actgggtggt tacgttttgt ttcaactaca actgtgattg tcttgtccat
    46561 tttgtcagat acaacacgtc caacaagaac tttacgatta ttgcgttcca ttgaaatttc
    46621 tccttcccta gtctattatt tcgcttcaga ttgaactgtt ttgatacgag cgatttgttt
    46681 tttaacttct ttcaagcgag ctgtttgttc caattgacca gtagcagctt ggaaacgaag
    46741 ttcaaacaat tcttttttca attcgttttc gcgcttcgcg agttcttctt gagaaagacc
    46801 acgaagttct ttaacaaatt cttttacttc attaagtttc atgccttctc cttattctgc
    46861 ttcacgtttt acgaatttac atttaactgg caatttgtgg ctagcaagtc gaagcgcttc
    46921 acgtgcaatc tcttcagata caccagcgat ttcgaacatc actttaccac gtttaactgg
    46981 tgctacccaa ccttcaggtg cccctttacc agatcccata cgcacaccga tagctttagc
    47041 agtgtatgat ttgtgtggga agattttaat ccaaacttta ccaccacgtt tcatgtaacg
    47101 agtcatggcg atacgagcag cttcgatttg gcggttagtg atccagtggc tagttgtagc
    47161 ttgaagaccg tattcaccga atgctacttc ttttccacct tttgcttcac cgcgcatttt
    47221 tccacggaac tcacgacggt gtttaacacg tttaggtact aacattggtt atttacctcc
    47281 tttagtgttt ttacgagctg gaagaacttc accacggtag atccatactt taacaccaag
    47341 tttaccgtat gtagtatctg cttcttccca agcgtaatcg atatctgcac gaagtgtgtg
    47401 aagcggaaca gttccttcag agtatccttc agcacgggcg atatctgcac cgttcaaacg
    47461 acctgatact tgagttttga ttcctttagc tccagcacgc attgcacgtt ggattgcttg
    47521 tttttgtgca cgacggaaag caacacgttg ctccaattga cgagcaattc cttcacctac
    47581 aaggtgagca tccaaatcag gttgtttgat ttcgatgatg ttgatgtgta cttgttttcc
    47641 agtcaatttg ttaagttttg cacggagtgc atcaacgtta gcaccacctt taccgataac
    47701 catacctggt ttagcagtgt gaagtgaaac gttaactttg tttactgcgc gttcgatttc
    47761 aatagttgaa actgctgcgt cagcaagttc tttttgaacg aatttacgga ttgcaagatc
    47821 ttcatgaagg taatccgcgt attctttttc agcataccat ttggcatccc aatcacggat
    47881 gatgccgaca cgcataccaa ttggatgtac tttttgaccc acgattttac ctccttattt
    47941 ttctgcaaca gctacagtga tgtgagctgt acgtttgttg attggtgaag ctgaaccttt
    48001 cgcacgtgga cggaaacgtt tcatagttgg tccttcgttt gcgaatgctt cagatactac
    48061 caaattagct ttatccaaac caaagttgtt ttcagcgtta gctacagctg agttcaaaac
    48121 tttcaagatg atttcagcag ctttgtttgg agtgaatgtc aagattgcga ttgcatcggc
    48181 tacgctttta ccacggatgt tatcaagaac aagacgtgat ttacgaggtg aaacacgtac
    48241 tgtacgagcc attgctttag ctgaagtaat ttctgccatt tatgttctcc ttattttcta
    48301 cgtgttttct tgtcgtctgc agcgtgacct ttgtaagtac gagttggtgc aaattcacca
    48361 agtttgtggc ctaccatgtc ttcttggatg taaacaggta cgtgtttacg tccgtcataa
    48421 actgcaatag tgtaaccaat gaaacttggg aagatcgttg aacgacgtga ccaagtttta
    48481 ataacttttt tcttttcgtc gttagcttga gcttcaactt ttttcatcaa atgctcatcg
    48541 acgaaaggtc cttttttaag actgcgtccc atttttatat tttctccttt aaatgttgta
    48601 ccacagcggc ttgcactcct atggagcgct accgagctgg cggatttact agttgcttaa
    48661 gcgactagtt taatattatt tctcgttgcg acgacgaacg ataagtttgt cagatttcgc
    48721 tttcttgtta cgagttttaa gaccaagagc aggtttgccc catggagtag atggtgcttt
    48781 acgaccaact ggtgctttac cttcaccacc accgtgtggg tgatcgttag ggttcattac
    48841 agaaccacga actgttgggc ggataccttt ccaacggcta cgtcctgctt taccaaggtt
    48901 tacaagtcca tgttgttcgt ttccgacaac accaactgta gcacggcaag ttccaagaat
    48961 catacgaact tcacctgatt gaagacgaac aagaacatat ttaccttcag aacccaatac
    49021 ttgagcagat gcaccagcag cacgtaccaa ttcaccacca cgacctggtt tcaactcgat
    49081 gttgtgaatc aaagtaccaa ctgggatgtt agcaagtgga agagcgtttc cgactttgat
    49141 atctgcttct ggacctgaaa cgatacgttg acctacttca agaccttttg gagcgatgat
    49201 gtatgctttc acaccgtcag tgtagtgtac aagagcgatg tttgcagaac ggtttggatc
    49261 gtactcgatt gttttaacaa ctgcttcaac gttgtcttta ttacgtttga agtcaaccaa
    49321 acggtaaaaa cgtttgtgtc caccaccttg gtgacgaact gtgatacgac cgttgttgtt
    49381 acgaccagcc ttgctcttca atgcaacaag caatgatttt tcaggagtgc ttgttgtgat
    49441 ttcagcgaaa tccaaagaag tcatattacg gcgaccgttt gttgttggtt tataaacacg
    49501 aattcccacg atatttcctc cttagattat tcagcttcag cagcaaacaa ctcgattgct
    49561 ttagaatcag ctgtaagtgt gatgatagct tttttagttt tgttagtaaa accagtgtaa
    49621 cgtccaacac gtttagcttt tggttttacg ttgattgtgt taacattggc aactttaaca
    49681 ccttcgaaag cagcttcaac agcttgcttg atcaaaagtt tgtgtgcacg agtgtcaact
    49741 tcaaatacat attttcctgc ttcaagttga gccattgagc tttcagtgat gacaggtttt
    49801 ttgataacat catacaaatt cattatgcaa gaacctcctc gattttagag atagctgctt
    49861 gtgtgacaag aagtttgtcg ctatttgcga tgtcaagaac acttgcagtt gtagcagttg
    49921 caactttcac gtttggaagg ttacgagctg aaagagctgc gaattcattt ccttcttcaa
    49981 ggataacaag aactttagaa tcgatgctca atgctgcaag aacttttgca aattcagcag
    50041 tttttggagc tgtaaatgaa agagcgtcta cagctacgaa tttgttttca gcaacttttt
    50101 cagagtaaac tgatttaaga gctaggcgac gaactttttg tggaagtttg tagccgtatg
    50161 aacgtggagt tggtccgaag acaacaccac caccacgcca ttgtggtgag cggatagaac
    50221 cttgacgagc acgtccagtt cctttttgac gccatggttt gcgtccacca cctgatactg
    50281 cagagcggtt tttaacagcg tgtgttcctt gacgaaggct tgcgcgttgg ctgatgatta
    50341 catcaaacac aactgattca tttggttcga taccaaatac tgcatcgcta agaacaactt
    50401 ggccagcttc tttaccagtt tggtcaaata atgttacgtt tgccattgtg actgatttcc
    50461 cctttcttta ttatttacca gctttaactg ctgatttgat agtgataaga gatttcttag
    50521 cacctggtac gttacctttg ataaggataa cgttcttttc tggaacaact tgtacaactt
    50581 caaggttttg aattgttacg cggtcgccac ccatacgccc tgcaaggttt ttacctttga
    50641 atacgcggtt aggtgcaaca ggtcccatag aacctggacg acggtggtaa cgagaaccgt
    50701 gagccattgg tccacgtgat tgtccgtggc gtttgataac accttggaaa cctttacctt
    50761 tagaagtacc agttacgtca acaacgtctc cagctgcgaa tgtttcaact gtaatttcag
    50821 caccaacttc caagccttca acgtttttga attcacgaat gaagcgctta ggagccgtgt
    50881 tagctttcgc tacatgtcct ttagcaggtt tgttgctcaa tacttcgcgt ttgtcatcga
    50941 aaccaacttg gatagcgttg tatccgtctg tttcaacagt tttaacttga agaacaacgt
    51001 ttggagttgc ttcaataact gttacaggga tcaattcgcc agcttcagtg aagatttgag
    51061 tcattcccac ttttttccct aagattcctt ttgtcatgag aaaatagttc cttttctata
    51121 tttttattca aaaagttttt aacgagcgtt ttttatgctc aagtctaaga tacaaagggc
    51181 gtcaaactca acgtgaaaat aggaaactga cacagtgtct agcagacgcg aggaagttta
    51241 tctttttcac accgagttta gcccgtgttc aattagattg aaacaccagc ctctgctctt
    51301 ttatatttta gattaaagtt tgatttctac gtttacacca cttggaagat ccaatttcat
    51361 caaggcatca actgtttttt gagttgggtt aacgatatcg atcaaacgtt tgtgtgtacg
    51421 catttcaaat tgttcgcgag agtctttgta tttgtgagtc gcacgaatga ttgtgtagag
    51481 gctacgttca gttggaagtg ggattggacc cgcaacttgt gcacctgtac gagtagctga
    51541 ttctacgatt tttgcagccg ctgtgtcaag cgtacggtgt tcgtaagctt tcaaacggat
    51601 acggattttt ttgtttgcca tctttttctc cttttcgtct atttaagata ataggctagc
    51661 tccacaagaa aaccgacgcg gtgttgcgtg gcaatgcaac cgagcgtgtc gcaacctctt
    51721 gcatcaaaac taaggctgta atttacagca ccataataga ataacacaaa tcccctgcga
    51781 ttgcaaggga tttgttaggt tttttcgttt tttggggatg aaactgtttt cctacttttt
    51841 ctttctaaaa ttagggattt tcttctatat ttcttctata cataagttaa gtcctcttga
    51901 catcaaattt atcttctgat tggtccacta agatatccgc cttagactca gtttctttat
    51961 agtatcgcag atactgctcc cgtctcatct gatggctagc caatataaag gatgcatcgc
    52021 gatttctcac agtcgtatct ctagcaaggc gtcgctttaa ttcggtctcc tcatccgtgt
    52081 agaaacagat ggttttttca aagagttcct tgggtagaaa gccaacagac atcccttcga
    52141 caatcaaaat tggtttggct ccagacaaga cctcactagc cttccaaggt tcttcaattg
    52201 tcaagacatc catacccgcc tgcaaggcaa ggatatctct ctgcaaactc tccagttcat
    52261 gcgccactga cagactggct gtcacctttt gattcggcgc gtccttgggt actaccaaat
    52321 agcgtccgga ggtgatatag ggatctgttt ctagcaaatt tactgtagta gaatctaaag
    52381 cttggtacaa ttcctgtgca aaggttgatt tacctgaagc tccatgaccg tatattccca
    52441 gtgtcctgac tttccccgtc tcgatctctg agactagttg gtctactaag tcttttttct
    52501 tcattctctc ttcacctgtt catagatttc ttttccgtca ttgagcttgt cccaaatcac
    52561 tacttgccca cttttgagcg atttttgcac atcgataatt cgttggttag acgaacctcg
    52621 aaactggagc ataagatttc tcttagttcg atcatatctt ccatcgacaa gaatgtcaat
    52681 cagtgacaag agttccagtt tatctggagt ttccaacatc atttcttccc aagtgtagcc
    52741 cgtccaggac cagatgtcct tgtctggcaa ttccttccga atccgcttaa caagtggcaa
    52801 gagaatccca gtattaagaa aaggctcccc tcctagtaag gttaacccct gaacataagg
    52861 ctgggcaaga tctgccataa tctgttcttc caactcagct gtatagggaa tgcctgcatt
    52921 gaaggaccag gtcgctacat tataacaccc ctcgcagtga aacatacagc ctgctacata
    52981 gagagagtta cgcacgcctt cgccatcaac aaagttaaag gccttgtagt caatgatacg
    53041 accttgacta agttcctcgc ttttccattc ttgtggtttt ggattattca ttcgctacct
    53101 ctatccaata acgctcgact ccattgcgag catcctcaaa tattccacca tttgctagaa
    53161 tgactgctct gctagcagga ttattcacgc tacaggtcac cagagctttc ttgatgttct
    53221 tttccttagc aacttgcaag ccctgacgga gagtctcttt tgcataaccc ttgcctcttt
    53281 cagatggacg gatggagtag ccaatgtggc cagcataatt cagtaacccc tcattcagtc
    53341 tcaatcgcag attcaaaaat cctagagcac aacctgattc accaaacaat acgaactgta
    53401 tcgatggaac gcgattttca ggtagccata ttcccatttc tttattacgg ttaccttcta
    53461 gccactcctc gtaagaaaaa tcctcaacat cccaaaatcc tccatcatgg gctgattggc
    53521 tcttttcaaa ctctgtcatc atatctaaaa cagctttctt atccgctaat cttggtctgc
    53581 gtaattccat ccttacctcc cactatgaga aaagcgagag ctggctctca cttttatttt
    53641 ttcttgttct tgtttaaaaa ttgttctctg aagctaagca agcgttcttt cttaccagct
    53701 ccacctttag agtgtttctc gtgataacgg gtcacttgtg cgcgcccctt atcgtctagt
    53761 tgatattttc ccatttcatt tctttctaat ttgttacttg atgcccagct attttaatcg
    53821 ttgaaccatt catatgtttg acacgcgcag cgatttcctt gtgacgcccg ttgaccatcg
    53881 gtcttgcttg aggattacct aggtagccac aagttcgctt caccacatct actgttttag
    53941 ggtcgctatt gccacagttt ggacaagcaa accctctctc agttggttca aaatcccctt
    54001 caaagtcaca cttgtagcaa cggtcaatcg gagtattggt gcctagatag cctacacggt
    54061 cataagcata atcccagaca gcttccaagg cctttggatt ttgctgaagg actggatact
    54121 cacaataatg gatgaaacca cctgacgcac ctgcttctgg atagactttc tcaaagtcca
    54181 atttttcaaa cggtgttgga tttttacgaa catcgtagtg gaaagagttg gtgtagtatt
    54241 ccttgtctgt gatatcagga atagagccaa acttgtctat atctagtcgg cagaaacggt
    54301 ctgtcagact ttcggatggt gttgagtaga tagagaaatg gtagccatat tggtctgacc
    54361 actcttctac acggcgtttc atatcgtgaa tgatgtctag cgtgaattcc ttagcatctg
    54421 gattactttc ccagctgtta ccaaagaaaa ctgtcgctac ttcatacaag ccgatatagc
    54481 ccagcgaaac ggtcgcacga caattcttaa agagctggtc aacactttct tctttaccta
    54541 gacgatggcc aaaagcaccg tactgataaa ggataggagc attcgctggt gtcgcctctt
    54601 tagtgcgttc gacacggtaa acaagagcat cttctgcgat attcattcgc tcgttgaaga
    54661 tttcccagaa cttattcata tcaccttcag actcaagagc aatacgaggc agattaaccg
    54721 tcacaacacc cagattcatg cgacctgaat tgacttctac accattttca tccttccatc
    54781 cttggaggaa agaacggcaa cccataggca ccttgaagga tcctgtcaag tcaacaatct
    54841 tatcgtaaga taaaacatct ggatacatcc gtttggttgc acactcaaga gccaactgct
    54901 tgatgtcgta gttgggagtt ccttcctcta aattaaggcc tcttttcaga gtgaaaataa
    54961 gtttaggaaa gatagccgta cggtgttctg aaccaagacc cttgatgcga atgtttaaaa
    55021 tagctttttg aatttctcgt tcaaaacgac tggttcccag accaaaacct agcgaagtaa
    55081 aaggtgtttg tccatttgaa gtgaagagag tattgatttc atactcaaga gattgcatgg
    55141 catcgtagat gtccttttgc gttttcttcc aagcgtaatc ttcccgtttt tcaggcaata
    55201 cccactcttc tgcatctttg agatgttttt gatagttctt ctctgcataa ggcgccaaaa
    55261 tttcatcgat acggtcagct gaacagccac cgtactggct agaagcaacg ttggcaatga
    55321 tttgagaaat ctgtgctgtc gcagtctgga tagacttggg actctctacc tctgcatttc
    55381 caatcttaaa accattttcc aacataccct taaaatcaat caaacagcag ttggtcatag
    55441 gggtataggg actgtagtcc aaatcgtgat agtggatatc ccccttttgg tgggcattgg
    55501 ctacgtgctt aggaagcatt tgcagtccga ttgatttccc aacaatccct gctgtcaaat
    55561 cacgctgagt gttaaagaca tcactgtctt tattagcatt ttcattgaca actgtctggt
    55621 ctttgttgag aagtttatga atactaaagt tgatatccgt cgcttttgag cgctcaaaat
    55681 ccctctgtgt ccgataagta atatactcct cagccagcgc atattctttg gcttcaagga
    55741 gttcatgttc tacgatattt tgaatttcgt aaatcttaat tccctgtgga aagcgactat
    55801 gaatttctgt aataattctc tcagtcagat cattgaggcg tttttcaacc aggggtgtca
    55861 catccataac cttgtcagct gccttgtgga gagccttgtc aatcttgtcc acatcaaata
    55921 caacacgtct cccatctcgt ttctcgacga agagagttgg caaaattgta attttttctt
    55981 ctagtgcaat catatgcatg ctcttttcta aaatgttctt tctaaaaagt attataccac
    56041 tctaaaaaga aaaatcaata tcttgtgtta aaaatcctaa aatttttaaa aatacacaag
    56101 atattgattt ggttatttaa ttttatagac tttaaactct gagtaatcag tctttacttg
    56161 ttggtaattt tcttttaaaa gtgtttccac ttcagaccag actaccacct tgtcatttac
    56221 cacaatcacc ttaggttgtt tttctttcaa gtcattaagt aacttattac gattttcctc
    56281 agttgctgta tagtgcaacg gggacaaaat cgccgatggc gacaagcgct cactcttacg
    56341 ataaagagtc gcagtatcat cccatgcata gatagcatct tccgtatttg tctcctcttt
    56401 taccaaatca gcaagacgat tacgttcttg ataaggtact ggataaagga caaacttcat
    56461 caaaaaagga acagacaaaa gatagacaaa cccaaaaact agcagatata gatttccttt
    56521 gaagaaacga ctccataggc taactggcgt ttctcttcgg cttcttctag cactacgccc
    56581 cctctgttga ctcttgatat tggttaccaa taaaagtagg aaaacaggga agacgaccat
    56641 caaataagaa gcgtgcagag gctcttgtga aaagaaaagt aaaatcaaac ctaaaattac
    56701 cacaagactt gcaggaaccg aaatgacgta tagtttaaag ggtttggatt gaaagagacc
    56761 taaaaagata caaaccacaa atcccaaacc aagggacaac aagccataaa aaaacatatt
    56821 ctctaaaatt ttagaagtaa aatcaaagcg aatactgtca ataggataac gaataccact
    56881 aatcgcatcc ccaaaacttc ctgttgcagc actatagtag gcagttggat aaaagacaag
    56941 tgaaaaacct aaagccactg caagaaactg ataaaaccca tgcgcaaagc gtctatgccc
    57001 aaggttaaag accaacaagc ctaaactcac tacagcaata aacaggagtg atgataaggg
    57061 agcaaagaaa aatccgcctg ccaaagctaa cccaatccgt acaaatccct tatcatggct
    57121 tggattgctt aggtaagccg caactaaact aaaggctgcg aataagaaag gaagcgctaa
    57181 aagagtcgca tagcctccac caaaagcaag acctgtaact agcatgtaaa aaatagtcac
    57241 cagatgtcca gcttggtctc cttgctctgt caaggtgtcc gctgatctaa aaaggaaaaa
    57301 tcctcctgct accaaggcta accactcaaa gatggcaaag aaaaatccgc cctgactcac
    57361 gtaagtcagc aaataataaa gcaaaccttg acttccataa tagtcgctgt aaatcttccc
    57421 tgtctgatga agcgcccaac ctgcataaaa atcctgcact tcttgtgcac tcattaagtc
    57481 gagtaatagc ggtactccta gagttatccc cgttacaagc gtactccata gtaaaatttt
    57541 caccaaagga agacgacttg attcacgatg atgcgattct tgctcgattt ggtattctag
    57601 aggttcacga ttctccttat gaacttcttc tactctacca tacacactca tatcgtttct
    57661 cctgttcaat ttatctgtct tagtatatca aaaagtccta ggattgtcag cttgcgatgg
    57721 ctgtttgagt gattttttgc atagtttcac aaaggtttca atttcccgaa agcggtgtaa
    57781 atgtgtctgt ttgaactcaa tcatatcatt cacttcttcc ttagttagca tgaattcccc
    57841 tcctctttct ttctattcgt aaaagacaag aaaaataggt cgggtttgta tcctaagttc
    57901 tagatttctt ataagatagg ggcgaatagc tgggcgattt cttttacaag cttttgatac
    57961 cagctcgttt tgatagaatg aggatagact tcctgagaat ctgcaaatat atttcgaaaa
    58021 tctcgggcga tttcccttat agaaggagtt ttatagagta agactgcatt ttcataatgg
    58081 tgtaccaagc tccggtagtc gagattgatt gtccccacca ccgcaaaatc ttcgtctacc
    58141 aacatctgct tactatgaat aaaacctgga ctatactcat aaatccgaac accagcagaa
    58201 agaaggtcgg gataagctcc tctcgtgact aactgaatga acttcttatc tggtatgtaa
    58261 ggggtgataa ttcgaacatc gacccctctc atagctgcat ttttgattgt ctctgttaaa
    58321 tcataatcta taatcaaata aggtgtcgta atatagaccg attccgttgc ttgattgatt
    58381 aaactctgat aaactttttt ccctacctgc gctcgaaaaa ttggcttggg tccacttccg
    58441 tatggaatgg ttaaaccgtc actcgggata gaatgatttt ctaaatgata ttgatcaaaa
    58501 tcactaattt ctcctcgatt aatgtaccaa gtggtcaaaa ataagcgtgt cagggctttt
    58561 actgctagtc cgtctaagcg aattccacta tccttccaat aaccaaatct ctcgacgtgg
    58621 ttaatgtact catctgccag attgacccca ccagtatagg ctatctgacc atcaacaatc
    58681 aatatttttc tatgatctct gttattataa gccactgtca aacgaggaat aactttattg
    58741 aatttatggg cctcgatgcc cagctgacga agtcgatgtg cataatctcc tgttaaagta
    58801 gccatacagc cgatatcatc atagagcatc ttaacctcta caccctgagc taccttttgc
    58861 tctagtatat ctagtatgcg attccacatc aaaccttctt ctataatgta atattccaag
    58921 aaaataaatt tctcagcctt tttaagatct tctaccatct ttttccacat agcttctccg
    58981 ttaggaaaaa atgtagaggc agtttgatca tagatgtcag cattggtatc catactcaat
    59041 aaggacttga tgactccata agctgccttg tcactttctt ttaatttctc ttttagtagc
    59101 tggctatttg cttcttggaa atgcatagag cctagcttct tcagttgttt gatttctttt
    59161 ttggacaatc gcctttcacc aaacatcaga tagagtaagg gaccaaatac tggcacaaag
    59221 gctactaaca accaggttac cttattctca ggagtcgtat tacggttgac tatcgaaatg
    59281 attgtgatga tactcagtag aatgaggaca gtaatccata aaatgggagc catgcgtcct
    59341 aaataaagaa agagaccaaa aacaagacaa agttcaagta gcataatcga aagactaaag
    59401 ccatacttgg acatcaataa ttgaaatttc ctatatttca tatcccctcc ttgatagttt
    59461 gaatgctaaa aacaagtaat tgatactagt ataacgcagg tattcggtag aaggcaagta
    59521 tttatgatta ttttctaggt aaaagtaaaa aggctgaaaa atccagccct ttctacttgc
    59581 atagtcttat ttagttaaaa cgatttggtc cgatgggtct ctatccatat catctacgac
    59641 gatttgatta ccgtttacag tgtaaatttt gacatcatcg ccaataatca tgcgttgatt
    59701 tgctgaatca aatgtaacct gtttgacttt ctgatccccg tcagattcga gctcagtcca
    59761 tgtaccagtc gttccagtta ccactaaagt gatacggtct ccgtcatcct gaccagtata
    59821 agtcccatca atatcggtcg gttgagcaac aggttggttt tgtactggag ccgcttgatt
    59881 ttggttacct cctgcatttg cagtattggt attctgttga gcaggttgtt gagcaggctg
    59941 ttggacctgc tgctgagcaa caggctgttg aacctgttga gctctttgtg aacaagctac
    60001 taagagacta aaacttgcta gagaagcaag agaaagtgtg aatcttttta atttcatgat
    60061 gaatttcctt tctgctacca atttagagaa attttctcta accagcaatt cccctagtat
    60121 aacaagttca aacaaggggg tcaatttatc tgctcacggt ccagcaggta gccccgtact
    60181 tctgagataa aatagagaga ccctgtaacg aacagcaagt cttgagcgtc tgccctttct
    60241 tcgaaactgc tgataaattc tcggcaagaa gaaactatat cgtaacctgt cacatccctt
    60301 tcgtccaaag ccccctgata gtcaaagccg gtcaccttga gttccacctg aggcaatttt
    60361 tcagtcaggt aacctaacat cccttgataa tccttccgtt tcaaggctcc aaaaaggatt
    60421 tgaggtcgat agccttcctg ctctttttct ttgataaact caaccaagcg agtcaaggca
    60481 gggaggttat gagcaccatc caagtaaatc tgtgggcgaa tacgctccaa gcgaccagcc
    60541 caatgggtct gttccaaggc ctttcttaca gcctgttcat caacagcttc ctttccttct
    60601 cccataaaaa gaagaaaagt ttgcaacgcc aaggctgcat tctcctgctg atagactcct
    60661 tctaagccta tgttcagctg tgaaaaattt agtaaagagc ttgaaaaatc accattcagc
    60721 attaaaaaat cttgacctgc ctgataaagg ttaacagcta aagattcggc ttttttctga
    60781 cagacaagcc tagcttctgg aggcaatttc gcaatcactg ccttcttacc agccttgaaa
    60841 ataccagctt tctgctctgc aattgcttct agactatcac ccaaggtttc ttgatggtca
    60901 agcccaatgg aggtgatgac agcaagctct ccagttacca cattggtcgt gtcaagtaag
    60961 ccaccaattc ccacttctaa taaaaccaaa tccacctctt gctccctaaa ataaagaaaa
    61021 gcaaccaagg tcagcatttc aaaaaaggac aactggtcag gagtttgcag aagcgttttc
    61081 tccatctcct tgacctgatc agccaaacgg ataaagtctg cgtctgctat aggttgccca
    61141 ttaatgcaga ttcggtcatt gatagagacg atatgagggg aggtaaaggt cgcaactttt
    61201 ttgccatgcc ccataaataa ttccctcata aaagcaatgg tagatccttt cccattagtc
    61261 cctgttacgt ggataatagg gtaagactgc tcaggatttc ctaacaaatc caccgcttgc
    61321 tgtattcggc ccaaacctga tcgaaaattc aaaccaatcc gactatgaag ccattcttct
    61381 acttcaaaca tacacatctc cttgacaaaa gtccaatcaa ttatcgcatt aaagtatggt
    61441 tactaataaa aacaaggcca ggattttcgt cccgacctct tacctggtta gctaataact
    61501 agccactatg aatgtgaata tgggctaaaa acatccacta gatgttccaa ctcttcccca
    61561 tttctgggag ttgggctgat acagtctccc agactgtatc actcctccat aaagctgttg
    61621 aagacttctt caatcatgtt ccattcgtct tctgagtctt ctgggattgg ttgcaattcg
    61681 ccttctgttc catcttcatt ttcaatgaat gagtaagctt ggatttcaac ttgtccgtct
    61741 tcgtcttctt ctgcgttaac tggtactaga agaacatagt ttttaccaaa ttcttctttt
    61801 ccatcaattg tcaaaaggat ttcaaacaag gtttcatttc cttgctcatc tactagtgtg
    61861 attagttcac gttcttcgtg gtcgtggtta tgatcgtgtg acatagcctc tcctttatat
    61921 taaaattttc tatctaaata attttgtaaa atcagctgag ctgctaactt atcaatgact
    61981 ttcttgcgct tattgcgact gatatctgct tgttcaatca acatgcgctc agcagccact
    62041 gttgtcaagc gttcatcctg atagtctaat ggtaaaccaa aaaactcttc tagctttgct
    62101 ccgtaggctt gactagcttc tacgcgcggt ccacttgtat tgttcatgtt tttaggcaag
    62161 cccactacaa atcgttccac cttgtaagta tcaaccaatt ccttaacgcg gtcaaaacca
    62221 aattggcctt gttcttcatt tatctggatg atttcaagcc cttgagctgt aaaaccaagc
    62281 ggatcgctaa tcgccacccc taccgttttt gaaccgacgt ccaatcccat aattctcata
    62341 ggttatagat cgactccttg tcctttgagg tagtagcgaa ccaattcctc aacgatttca
    62401 tcacgctcat acttacggat ttgatttcgt gcattattat aacgaggaac gtaggcaggg
    62461 tctccactca atacgtaacc tacgatttgg ttaattgggt tgtaaccctt atcgttcaac
    62521 gaagcataaa catctgtcaa agtttcgcta atttcttttt tattggaatc gtccaattta
    62581 aaacgtactg tttcttcagt aaatcccatt ctaacaccct ctttccttag aatagtacca
    62641 ttatagcata attccttacc ttctacaatt caggcagtct atttatttgg attttctatt
    62701 gttctgtcgc gccatttgcc aatctatctg aaatatattt gcttggttca tttttcaaaa
    62761 gattttccaa accaatattc ttcagatgtt ccaactggga agccttcttg acatccagaa
    62821 cttgaaaatc aaaactagtc gttgtttgaa gttccgttgc gctcaatagt tttgtttcaa
    62881 gtttgaaacc tgccaattta cgagcttcaa tgatagactt atccttctcc tccgcttcaa
    62941 gaagagcttt ttgagtttcc tccactccat gttggtcaat ataagtcttc aactcatcag
    63001 agactggacg tttttgttgg atagttagac tcaaaaaagt cttgtccttg tatgtaatgg
    63061 tttgtgtctg ctggctacca tcatctgact tgggcaagac caaagtctta gttacaactg
    63121 tattcttctc agcattttca ataactggca atgccgactg aagcgtatct ttttctgttt
    63181 ttgtagctgg tccagtttct tttttctgtc cgcaaccaac caggacaaaa aggaaagcta
    63241 gactaacaag aactattttt ttcatttctt tcttctttct ttttgaaatt aaaatagaat
    63301 aagactggga agtgctccca gccttgatgt ttatagagct gcacgcaaac gtgcttctgc
    63361 attttctaca ttacggacag agcgtggtag gaaggcacga atatcgtctt ccttgtagcc
    63421 aacttgcagg cgtttttcat ctacaaggat tgggctcttt aaaattctcg gtgtttccat
    63481 aatcagattg agaacttcat tgacactcaa atcttcaata tccactccaa gggctttggc
    63541 atagcgattt ttagacgaaa cgatgctggc tattccgtta tctgttttgg ttagaatatc
    63601 cagtaattct tctctcgtaa ttccttcttt accaaggttt tgttctttat aacttaactg
    63661 gtgggcattg agccaggttt ttgctttttt acagctagta caacttgaga ctgtataaat
    63721 tttaatcatg tacctacccc tttcgctaca tgttactatc agtttagtct attataccat
    63781 aaaaaacatc cgacttgcga cctattttta at
//
LOCUS       DAVBCR010000003        53160 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCR010000003 DAVBCR010000000
VERSION     DAVBCR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.003"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..463)
                     /locus_tag="VQ508_000146"
     CDS             complement(<1..463)
                     /locus_tag="VQ508_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489211.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VQ508_000147"
     CDS             complement(453..650)
                     /locus_tag="VQ508_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489212.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(654..1238)
                     /locus_tag="VQ508_000148"
     CDS             complement(654..1238)
                     /locus_tag="VQ508_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489213.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1646..2071)
                     /locus_tag="VQ508_000149"
     CDS             complement(1646..2071)
                     /locus_tag="VQ508_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489214.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2064..2558)
                     /locus_tag="VQ508_000150"
     CDS             complement(2064..2558)
                     /locus_tag="VQ508_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489215.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(2604..2810)
                     /locus_tag="VQ508_000151"
     CDS             complement(2604..2810)
                     /locus_tag="VQ508_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489216.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(2797..2958)
                     /locus_tag="VQ508_000152"
     CDS             complement(2797..2958)
                     /locus_tag="VQ508_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489217.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3323..4279)
                     /gene="lytA"
                     /locus_tag="VQ508_000153"
     CDS             complement(3323..4279)
                     /gene="lytA"
                     /locus_tag="VQ508_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2489218.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(4655..6025)
                     /locus_tag="VQ508_000154"
     CDS             complement(4655..6025)
                     /locus_tag="VQ508_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2489219.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6332..7498)
                     /gene="recA"
                     /locus_tag="VQ508_000155"
     CDS             complement(6332..7498)
                     /gene="recA"
                     /locus_tag="VQ508_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2489220.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(7553..8809)
                     /locus_tag="VQ508_000156"
     CDS             complement(7553..8809)
                     /locus_tag="VQ508_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2489221.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(8893..9909)
                     /locus_tag="VQ508_000157"
     CDS             complement(8893..9909)
                     /locus_tag="VQ508_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2489222.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(9917..10435)
                     /locus_tag="VQ508_000158"
     CDS             complement(9917..10435)
                     /locus_tag="VQ508_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489223.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10425..10868)
                     /gene="tsaE"
                     /locus_tag="VQ508_000159"
     CDS             complement(10425..10868)
                     /gene="tsaE"
                     /locus_tag="VQ508_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2489224.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(10954..11574)
                     /locus_tag="VQ508_000160"
     CDS             complement(10954..11574)
                     /locus_tag="VQ508_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489225.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(11957..12820)
                     /locus_tag="VQ508_000161"
                     /pseudo
     CDS             complement(11957..12820)
                     /locus_tag="VQ508_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            12985..13179
                     /locus_tag="VQ508_000162"
     CDS             12985..13179
                     /locus_tag="VQ508_000162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489226.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            13494..13790
                     /locus_tag="VQ508_000163"
     CDS             13494..13790
                     /locus_tag="VQ508_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2489227.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            13893..15908
                     /locus_tag="VQ508_000164"
     CDS             13893..15908
                     /locus_tag="VQ508_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2489228.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            15910..16542
                     /locus_tag="VQ508_000165"
     CDS             15910..16542
                     /locus_tag="VQ508_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489229.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            16683..16961
                     /locus_tag="VQ508_000166"
     CDS             16683..16961
                     /locus_tag="VQ508_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2489230.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(17470..17883)
                     /gene="ndk"
                     /locus_tag="VQ508_000167"
     CDS             complement(17470..17883)
                     /gene="ndk"
                     /locus_tag="VQ508_000167"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2489231.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(17994..21671)
                     /gene="rpoC"
                     /locus_tag="VQ508_000168"
     CDS             complement(17994..21671)
                     /gene="rpoC"
                     /locus_tag="VQ508_000168"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2489232.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(21704..25315)
                     /gene="rpoB"
                     /locus_tag="VQ508_000169"
     CDS             complement(21704..25315)
                     /gene="rpoB"
                     /locus_tag="VQ508_000169"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2489233.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLKEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            25731..25801
                     /locus_tag="VQ508_000170"
     tRNA            25731..25801
                     /locus_tag="VQ508_000170"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25763..25765,aa:Cys,seq:gca)
     gene            complement(25999..27330)
                     /locus_tag="VQ508_000171"
     CDS             complement(25999..27330)
                     /locus_tag="VQ508_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2489234.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(27621..28445)
                     /gene="endA"
                     /locus_tag="VQ508_000172"
     CDS             complement(27621..28445)
                     /gene="endA"
                     /locus_tag="VQ508_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2489235.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(28484..28672)
                     /locus_tag="VQ508_000173"
     CDS             complement(28484..28672)
                     /locus_tag="VQ508_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2489236.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(28665..29948)
                     /gene="murA"
                     /locus_tag="VQ508_000174"
     CDS             complement(28665..29948)
                     /gene="murA"
                     /locus_tag="VQ508_000174"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2489237.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(29972..30202)
                     /locus_tag="VQ508_000175"
     CDS             complement(29972..30202)
                     /locus_tag="VQ508_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2489238.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(30260..31297)
                     /locus_tag="VQ508_000176"
     CDS             complement(30260..31297)
                     /locus_tag="VQ508_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2489239.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(31281..31769)
                     /gene="coaD"
                     /locus_tag="VQ508_000177"
     CDS             complement(31281..31769)
                     /gene="coaD"
                     /locus_tag="VQ508_000177"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2489240.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(31759..32298)
                     /gene="rsmD"
                     /locus_tag="VQ508_000178"
     CDS             complement(31759..32298)
                     /gene="rsmD"
                     /locus_tag="VQ508_000178"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2489241.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(32363..33355)
                     /locus_tag="VQ508_000179"
     CDS             complement(32363..33355)
                     /locus_tag="VQ508_000179"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2489242.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(33467..34150)
                     /locus_tag="VQ508_000180"
     CDS             complement(33467..34150)
                     /locus_tag="VQ508_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2489243.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(34206..34946)
                     /locus_tag="VQ508_000181"
     CDS             complement(34206..34946)
                     /locus_tag="VQ508_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2489244.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            34980..35258
                     /locus_tag="VQ508_000182"
     CDS             34980..35258
                     /locus_tag="VQ508_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2489245.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            35336..36262
                     /gene="yidC"
                     /locus_tag="VQ508_000183"
     CDS             35336..36262
                     /gene="yidC"
                     /locus_tag="VQ508_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2489246.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(36337..37131)
                     /gene="pflA"
                     /locus_tag="VQ508_000184"
     CDS             complement(36337..37131)
                     /gene="pflA"
                     /locus_tag="VQ508_000184"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2489247.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(37257..38507)
                     /locus_tag="VQ508_000185"
     CDS             complement(37257..38507)
                     /locus_tag="VQ508_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2489248.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(38574..39401)
                     /gene="purR"
                     /locus_tag="VQ508_000186"
     CDS             complement(38574..39401)
                     /gene="purR"
                     /locus_tag="VQ508_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2489249.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(39682..40623)
                     /locus_tag="VQ508_000187"
     CDS             complement(39682..40623)
                     /locus_tag="VQ508_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2489250.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(40613..41869)
                     /locus_tag="VQ508_000188"
     CDS             complement(40613..41869)
                     /locus_tag="VQ508_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2489251.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(41871..42533)
                     /locus_tag="VQ508_000189"
     CDS             complement(41871..42533)
                     /locus_tag="VQ508_000189"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2489252.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(42496..43152)
                     /locus_tag="VQ508_000190"
     CDS             complement(42496..43152)
                     /locus_tag="VQ508_000190"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2489253.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(43163..44041)
                     /gene="rsgA"
                     /locus_tag="VQ508_000191"
     CDS             complement(43163..44041)
                     /gene="rsgA"
                     /locus_tag="VQ508_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2489254.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(44634..45407)
                     /locus_tag="VQ508_000192"
     CDS             complement(44634..45407)
                     /locus_tag="VQ508_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2489255.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(46097..46969)
                     /gene="rsmA"
                     /locus_tag="VQ508_000193"
     CDS             complement(46097..46969)
                     /gene="rsmA"
                     /locus_tag="VQ508_000193"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2489256.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
     gene            complement(47220..47438)
                     /locus_tag="VQ508_000194"
     CDS             complement(47220..47438)
                     /locus_tag="VQ508_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489257.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(47506..48147)
                     /locus_tag="VQ508_000195"
     CDS             complement(47506..48147)
                     /locus_tag="VQ508_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489258.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(48149..50179)
                     /locus_tag="VQ508_000196"
     CDS             complement(48149..50179)
                     /locus_tag="VQ508_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2489259.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(50448..51347)
                     /locus_tag="VQ508_000197"
     CDS             complement(50448..51347)
                     /locus_tag="VQ508_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489260.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(51419..51979)
                     /gene="rnmV"
                     /locus_tag="VQ508_000198"
     CDS             complement(51419..51979)
                     /gene="rnmV"
                     /locus_tag="VQ508_000198"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2489261.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(51979..52752)
                     /locus_tag="VQ508_000199"
     CDS             complement(51979..52752)
                     /locus_tag="VQ508_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2489262.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(53057..>53160)
                     /locus_tag="VQ508_000200"
     CDS             complement(53057..>53160)
                     /locus_tag="VQ508_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2489263.1"
                     /translation="NTGSASSAALASLGLVVATSGFALLGRKTRRRK"
BASE COUNT        15587 a        11893 c         9734 g        15946 t
ORIGIN      
        1 aaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatgtaagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcaggatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc ataaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaaaggca
      841 atccacgtta attgttcgct aggtgttaat tccttaggga tttcagtatc attcgcattg
      901 agtactagtc ccaatggagg gaagaagagg tttcccttaa agtctacaaa cggaactcta
      961 gcttgaagta attgcttttt ttcactgtct gataacttcg aaaacaccaa aacaacatcc
     1021 atattggctt tttcacccat agtgcgagcc tgagtaacaa atgaactcaa actccccctt
     1081 ctcttttctt taatcaataa aaatgattcc ttatgaaaag tcatttgagt atactcaaac
     1141 ctttttacaa aactgattgg taaagtcaaa gtcagttcct caatactgac atcaacataa
     1201 aaggattgaa aaattgacat tatctttttg acattcatgt tttttcctta ttctatcatt
     1261 tttattatat tttatcattc ggtataataa aatataagtt ttttgataaa ataaccaaaa
     1321 actactcgct atcccctctc acattaccct acatatcgtt tgacatgcga ctgatagttc
     1381 aggaaaactt caaggagaac ttttctctca tccactatgc aggacttact atttcacttc
     1441 tactcctata ggctcaattt gacacttttc tttttcgaat tgctcatatg cctcctgaat
     1501 agtgagttcg atttatccaa atttattttt gtctattttg atgaatactt ccacgaagat
     1561 agtctgaaaa tgaatttgca ccaattttcc ttctcttttt aagcatatta ttccagatat
     1621 gattgccctc ttgttcagta ataaattacg ctttaaacgc ttcaatcagt atatctcctg
     1681 ttgtcatatg ttctaaagaa aattcttcta catatggttt aacatctctt aggttattac
     1741 ttcctaatat cccattatgc tttttcgcta agaaaataga agtcgcttct cccttaccaa
     1801 taatcttgtt actatcataa tttcttgtta aatctctata taatgcgtat tcttcagttc
     1861 caatgtctat gctcacaatc tcagctgaac ccttagttac caactggtct atcctagatt
     1921 ttaaatgggg aattgtaggt atattgattt catcatacac ctcttgtgga ataacaattt
     1981 tacccgaata gagcttttct aaaagatgtt cagtgccaac ccataaaaat gctgaaatac
     2041 aatctgcatc aataaataca cgactagtca aacgacaact ccccctcttc atctagccca
     2101 tatacaatat catatctgaa agcatctagt aacagttcct catacttccc ttgcgaaatt
     2161 ctgttatttt ctaaaagttg ttcagtcgat ttaatatagt gccctaatac catttccttt
     2221 ttactttctg acaaaggacg atataaactt gtatcatagc ctaatcttga agctgtctct
     2281 ataacactaa tatccatatt tttaatttct tctgcatcaa ggtatccatc atttctcaat
     2341 ctatataaca tagctttatg actgatacca taaaactgac ccaattttat aatatcttct
     2401 acttcaagat gagttctatt ggcattttct ctgatttcct caaccatcct atacagtgaa
     2461 gatgggaaaa ttaaaaaata agaagcaaac tgatccgctt ttctttcagt ttcatctcct
     2521 tcaccaatca agataagact gactgaactc ttcttcacct catcataata aagatgatac
     2581 agtccatgtg ctaaagaaaa tctttacctt cctaatggca tgtctgaatt gactgcaatg
     2641 agactgaact gagttccttt ataacagact ccgctaatat tctttccgag tccataaaat
     2701 accagcgtca aattttctat cttttgtacc aatttaaaaa tatctatcgg cgattcacca
     2761 tcagctccca acttttttct aagatttgaa gctttattac ttaaatccat tcgtgatatt
     2821 cctttcacct ttttccattt tggcaatcat gctttgattc aaagacaaat actcagcaat
     2881 cttatcttga gtaagatttg aagatattct aagttctttt attctttttc caacatcaca
     2941 tacatttatc atattcatac acctctcata aaaagaatag cacattcttg tcataatttt
     3001 ttaaataaaa aaattatgac aaaacaagga agcaatttat tgatgctact taaaaatcta
     3061 aaattgatga tattaaacct atttgatgag ttcctatcaa aaatcgtatc ttcaacctca
     3121 aaacagtact taaagctatc cgactcggtt tacattgtca aatttagatt ttatttgagc
     3181 ataactttct agtttgcttt ttgatttttg tttaatatag taacaaaaat tgaaggaaat
     3241 atctccacaa gaaaacgcat actattaagc tttttcaaga cctaataata tgcgctgttc
     3301 tgatttgaaa gacattccat tattatttta ctgtaatcaa gccttcaggc tctactgtga
     3361 agtctggctt gtctgccagt gttccgtctg gtttgaggta gtaccagcct gttccgtccg
     3421 ctgactggat aaaggcattt gataccatgg cgccttcttt agcgtctaag tagtaccaag
     3481 tgtccttgta cttgacccag cctgtcttca tggcaccttc ttcgttgaaa tagtaccact
     3541 tatcagcgat tttcttccag cctgtagcca tttcgcctga gttgtcgaac cagtaccagt
     3601 tgccgtctgt gtgcttcctc cagcggtctg caagcatata gcctgaactg tcaaagtagt
     3661 accaagtgcc attgattttc tcaaacttgt cttttggata agagccgtct gaatgtacgt
     3721 accagtagcc agtgtcattc ttctgccagc ctgtttcaat cgtcaagccg ttctcaatat
     3781 catgcttaaa ctgctcacgg ctaatgcccc atttagcaag atatggataa gggtcaacgt
     3841 ggtctgagtg gttgtttggt tggttattcg tgcaatactc gtgcgtttta attccagcta
     3901 aactccctgt atcaagcgtt ttcggcaaac ctgcttcatc tgctagattg cgtaagagtt
     3961 cgatataaag gcggtagtcc gtcatgaact cttctttggt tgaatggctt tcaatcagtt
     4021 caaccgctgc ataggtctca gcattccaac cgcccccaac gtcccaggca ccattatcaa
     4081 caggtcctac ctgcatgatg caaccgttcc caacaatgtg cgagaaaaaa cctaattctg
     4141 ggtctttccg ccagtgataa tccgcttcat tctgtacggt tgaatgcgga ttcccagttg
     4201 agtgtgcgtg tacttgccta tatggttgca cgccgacttg aggcaaatct gttcttaatt
     4261 tactcacatt aatttccata ttctactcct tatcaattaa aacaactcat tttttacaat
     4321 ccagaaccag aaactccttt atttctacct tacaaagaag acaatcttag tcacgattaa
     4381 gcttgtagat agaacctcaa aacgcactat tttgacactg taaataggac tgacaaggtc
     4441 tgcattctat ctacaataac accccagact aaaaagcttt tcaaaagtat atttttacag
     4501 tctctatatg tccttttcat aaatactata ctttattata tcacataaaa gaagtcaaaa
     4561 gtctgttaaa ctattttcaa caccaaacta aagaagagaa cacaagtttt ttcgatgttc
     4621 actagaggaa atggatttta ttcagtaaat ccaactagga ttgcactttg gttgccaaaa
     4681 ttgcctttcc ttcttttatc aagggatgac ggaatagtga gaagtacagt tgaactgtca
     4741 tggcaaccca gataagaggt tcacaaagga taacaccctt atatcctgcc caaggaataa
     4801 tcaaaaccac aaaaacgatt tttccgatta gttcaataaa gctagaaact agaggaagga
     4861 tcttctgccc caagccctgc aagcaattgc gataaatcaa caagaggctc aaaatgggat
     4921 aaaaggctga actgatttgc agatagagac ttccattttc tatcaagtaa ccatctgtcg
     4981 aactagccaa gaaggaaacc aaggctggac tggcaaaaaa gaggaaaata caaacaaaaa
     5041 ctgcccagga tatacttaaa cgactgccga ttcgaagacc ttgaacaatg cggtcaggtc
     5101 gcttagctcc tagattctga gaagcaaagg tcgtcattga tgcagaaata gcggtcatag
     5161 gaagaagggc aaaggtcata atgcgtcgag ctgccgtctg ggcactaata atcactgcac
     5221 caaatgtatt aacagaaaac tgtaaaatca cactgccgat agatacaatt gaactcatca
     5281 agcccatagc caaaccttgc tccaagagat ccgcgtacaa gcttttgtcc catttgaaat
     5341 gtttaaactg tggcaagagt tctggcacac ttttacgaat ataataaaag cagagaaccg
     5401 ctgataaacc ttgcgaaata atggtagcaa gtcctgcgga ttgaactccc agatgcaatt
     5461 gcgtaataaa atagagatcc agaaccacat taaccaaggc agagaaaatc agaaatccca
     5521 gggctgctag actgtcacca atagaccgca acaagcctgc aaaaagatta taagcaaagc
     5581 tgacacctac acaggtcaca atcatagaaa tatattgata agattgagga agaatttctg
     5641 caggagtatc taagtattgc aagagatgat acaagccaag aaagcccagc aacataacta
     5701 gaatgctcaa aagagcacct aaaatccaag tagctgctac tgcttccttg attttagtga
     5761 aattccgagc cccataataa cgagcaatga caatccccat gccattgcca acaccaagtg
     5821 taaaacctac aatcaggtca aaaatcgctg tcgtcgctcc tactgcagcc aaggattctt
     5881 gaccaagaaa tcgtccaaca atcaagacat cagcagtgtt atagagctgt tgaaaaatat
     5941 ttgatagcaa aattggaaag gtgaagctta agagcgaggg aagaatcgga ccatgtatca
     6001 ggtccactgt tcgtttttta ttcataaagc tattatatca gctttccaga ggagcgacaa
     6061 gaaacagcaa acttatatat agcggaatga aataaggaaa gtcaaatcga tttttaacaa
     6121 tgttttagaa gtagatatgt actattctag tttcgatcta ttatagttgg ttgagtttgg
     6181 aatagtacgc tgtagctgct aaaacatttc tagaaattaa tttgactttc ctaatagagt
     6241 tgttcatatc ttatttcaag ctactatata taatctaaaa acttgaatca aaaatcgaaa
     6301 aagtagcgga tttctccact gctttaacag cttattcttc aatttcgatt tcaagttcat
     6361 cgcctaagtc aagcggaact tcttcattca ctgcttcttc tttctttggc tcatcttttt
     6421 tgttttcagt atcttgttct gaaacttctt ctccatcaat caagccaaat ttagaacgga
     6481 cttgcttatc aatttcatca aagatttctg ggtgctctgc caagtatttc ttagcattct
     6541 cagaaccttg cccaattttt tcatctttgt aagaatacca agcccctgct tttttgataa
     6601 tatccaaatc gcttgcaatc ttcaaaagct caccagtctt agaaattcct tctccgtaca
     6661 taatttcaac tacggcttcc ttaaacggtg gagctacctt attttttaca accttaatct
     6721 tagtttcttt accgacattg gtttcttttt ggtcaccagt tcccttaatt tgtgtattac
     6781 cacgaacatc caagcggact gaagcataga atttcaaagc acgtccgccc ggtgttgttt
     6841 ctggatttcc aaacatcact ccaacttttt cacgcaattg gttgataaaa atggcaattg
     6901 ttttggtttt attgatagag gcgccaagtt tacgcatggc ctggctcatc atacgagcct
     6961 gcaaaccaac atggctatct ccgatatctc catcaatttc cgcacgagga acaagggcag
     7021 caactgagtc gactacgaca agatcaactg cacctgagtc aatcaatttt cccgcaatct
     7081 caagaccttg ctctcctgag tctggttgag acaagagcaa ttcgtcaata ttgacaccaa
     7141 gggccgcagc ataagctgga tcaagggcat gttccgcatc gataaaagca gcaatcccac
     7201 cttctttttg cgcttgtgca actgcatgaa gggcaaccgt tgtcttacca gatgactctg
     7261 ggccatagat ttcgatgata cgtcccttag gataaccacc tgagccaagg gcaatgtcaa
     7321 gagctaaaga acctgagctc atcacttgca ccttttgctc cgcacgttca cccaaacgca
     7381 tgattgatcc tttaccaaag tctttctcaa tcaatttaag agcgtcattc aaggcctttt
     7441 cacgttctgc cccaaatttt tttgaaattt cttctaattt ttttggtttt ttcgccattc
     7501 tattctccta cattctaatg atcctcagac ctatctacta ttatatcaaa aattagtcac
     7561 ttaataaagc cttgcgaact aggttaaagg catgcataac cgcaatgtga cgtacatctg
     7621 ctcggcttct gcctccaata ttaaccttga taacctcagt tccttgatct tgcgccaagc
     7681 ctatgaagac tgtcccaact gggtgtcctt ctaggctatc tggtcctgcc actccagtca
     7741 aactaattcc aaaatcagac tgggtcttgc ttcgtgcctg ctcagccatc ttctgagctg
     7801 taaattcaga caccacacca tgttcttcca aattcttggc aggaatatcc aacatccttg
     7861 atttttcctc caagctatag gtcacaaaac cacccttaaa tatacttgaa attccagaaa
     7921 aattcgccac ggtagcttgg aaaagacctg ccgtcaaact ctctgcagcc gcgatggttt
     7981 tcccttgcct tttcagttct tctaccacaa tgctggctaa actagtttct tccccataac
     8041 catagcaaaa gtctcgtaaa gaaattcctt cgaaagtctg gcagtccaag atttgatttt
     8101 ccaagatatc cagcgcttga ttcgcctctt cttgactgct agcctttgtt gacagacgta
     8161 gagtgacttc tcctgtcttg gcataagggg ccaaggtagg atcgacctga ttatcaatta
     8221 aatcagccaa aatcgtaacc agctggctct cgccaatccc aaagaaacga agaactcggg
     8281 aatacagctt gctccctgtc atcaacttgg gtagaagtag gtttaagacc atgggtttca
     8341 attcacttgg cggacctgga aggacgacat aggtcactcc gtctacttct aattttcctc
     8401 caacagccag tcctgtttcg tttggcagtg gaatcgctcc ttctacaatt tgagcttgtc
     8461 tttcgttatt cggtgttcgg gcatagtctg gtcgcagggc aaaaaagata tccaacttct
     8521 cctgagcctg aggatcaaag actaatgctt tccctaaaaa tttagctagg gtttgtttgg
     8581 ttaggtcgtc ctcagttggc cccaaaccgc ctgtcaaaat caccagactg ctacgttgac
     8641 tggcaatctc aagcaaagac aagagacgaa cttcattgtc tcctacagcc gtctgaaaat
     8701 atacatctac cccaatctca gctagttttt ccgacaaaaa ctgggcattg gtgttgacaa
     8761 tctgtcctgt caaaatctct gttccaacag caatgatttc tgctttcatg tttcctccta
     8821 cctatctatt cgtatttttt tgaaaaaatc gcaggaattt tcctacgatt gattttttat
     8881 ttgtatcaaa agttaattat cttcatcacc aacaggtgct ctgccaaata aatcttcaaa
     8941 taaaaccgca ttggtttcaa gctgagtaac ttcttcttgt cccaaagaac gtcggagtat
     9001 attttgcatt tccaacatat gtgctcttga aacaatctgg taagaaacac cttgaagtat
     9061 ctctccttca ccctgcaact gctgagtttc aatggtttta aatgaatctt tatagcctag
     9121 caagttaggg atactttttg cagacaaatc aatattggtc tgcatattgt cactcaaagc
     9181 ttttagaatc tcttgataat gaccaacgct atttaaactg agagcttttt ccatgacttt
     9241 ttgaataact tcacgttgac gtttttgacg accataatcc ccctcaggat cttggtaacg
     9301 cattcgtgca tagactaggg ctttttctcc cccaatatgt tgctccccaa caccgataga
     9361 aatagtatta aattcttctt ggtcactgat agaaattggg aaacctagga tattattgac
     9421 tgtaatacct cctactgcat ccactagttt ttgcaaccct ctcatattga ccatcacata
     9481 gcgatcaata tggatattca tcattttttg aatggtttct atagcaagct ctgctccacc
     9541 atctgcatat gctgagttca gtttcgcttc atgagcctga ccattccctg attcaatgcg
     9601 cgtcagaata tcccgctcta aactcatcat tgttgttttt ttcgttttag gattcactgt
     9661 catcaagatc atgctatcac ttctaccgac ccaagtttca gttcgttcaa catttccggt
     9721 gtccactccc attaacagaa tggttagagg ttcagtcgct tcaataacct tggtttcttc
     9781 gccgattttt ttataggttt tagctaaggt ttctgtccct tgttgataaa tagtataagc
     9841 aaaaacacct actcctacta cagttacaga aagtaaagct agcaccattc caataatttt
     9901 tttaaccata tttctactaa cctatcagtt tacccatcaa gtaaacatcg ataaatttcc
     9961 cttcttctat atatgcccca cgctcttggc taccttcaat gacaaagcca tgcttttgat
    10021 aaagatggac tgctgcttga ttacgagttt ggacagtcag ttggagacga cgcagaatgc
    10081 cacttgcttg tgcccactct atcgcttctt ctagcaacaa acttcccaag ccattattcc
    10141 aatatctttt tccaatcaca atgaagagat ctccaatatg acggactctc ttacgctgat
    10201 cagctgtaat atttacaata ccagcaattt tgccatttaa gaatgcaagt aaggttatct
    10261 gattgtccga actagcttgc ttgttgagga atatttccat ctcctcacta gtcaagagaa
    10321 taccatctcc gtctaggctg gtaaagtctg tctccaaact cacacgattt aaaaaggcca
    10381 ctaattcagc tgcatctttg ggctctgctt ccctaatgag caattcatac tccatattga
    10441 agctcctcta acaatttctc agcacgcaaa ccctttgcct gaaaatttaa acggcgtcca
    10501 tctgcttctt ttagaatttc caattctaaa taagcatctg gcaaggcatc tcctaagaga
    10561 tttccccact caataacagt cacgccgcca ccaaagataa actcatccaa gtcgatagaa
    10621 tcagcatctc cttcaatacg ataaacatct aggtgataaa gtggaagtcg accttcatac
    10681 tctctcacga tagtataggt gggactttta atcatttgag aaatctgtaa tccttttgca
    10741 agtcctttag taaaggtcgt tttacctgca cccagttctc cagttaagat taaaacatca
    10801 ttctttgcta atagatggcc caaacgctcc cctaaggctt gcaactcttc ttcatttttt
    10861 gtgtacatac tcttattata ccaaaaactt ttcttttgtg tctattttcc tactaaactt
    10921 atcatcataa catccataaa aaacaggctt tctctaaaag aaaatgagcg taacaatgac
    10981 caatacaaga tctcggaaaa tatgaccata aaaggaaact tccttcttaa ccgaatttgg
    11041 gacaagatag gctgcaaaaa acaagcccag tccaatataa atcagaagtg agacaatggt
    11101 cattggattt cttaagaaaa gaagtgttgc taaaatagtc accaacactg tcttttttct
    11161 gtctagcata gcaagaaaat cgcgcacgta ttttttcaag ggtaaaaaaa tcagcaaatc
    11221 tagcccaaat aggaaaaaga aggatggcaa taaaaagtca actaattctt gctgcagcgt
    11281 atttttgatg aacaagttat ctgacaaaac aagaacagct cctaacaaat taattaagag
    11341 taacatactg taaaaaagct tcaccgactt cttactggct aggacactat ggacttcttg
    11401 cttacgggta taaagataat ttactccagc acagattcct gaaacgaaaa ccatgctccc
    11461 gatgaaaaaa gctgtacttt gtttaaagga caagatgcat tccttccata ggaaacagct
    11521 actcaaactg atttgaatta aagctaacaa aaataagatt ctcattgatt tcatcttctc
    11581 tctcccttcc taccaatcat tatactagga gaaaagagag aactgtttct aatcttctca
    11641 aatgtctctt taagacgcta aacaaacact agagactaat actcaatgaa aatcaaagag
    11701 caaactaggt agctagccac aggttgctca aaacactgtt ttgagattgc agatagagct
    11761 gacgtgattt gaagagattt tcgaagaata taaatttgaa atcatgaaaa tccgtcaaac
    11821 gggtggttgt tttgtctcgc acctcacgga gcgagacgga ctcagagtca cataattata
    11881 aggctgatag tattaatcta actatcagcc ttacaggtta tttaacgttt cagaaaaact
    11941 ataatgtcaa gattaactaa acagtatcta attccttcaa ataattttct atcttcatca
    12001 gcattaaagg attgttataa atcttgcata gctctcttgc ttctatataa taatttttga
    12061 cttgttctct gtctagaaat ttggctccag catttcctac aagaataagt agaggagcca
    12121 attggtagct tgtctgtctt tgtttacaga gttcgatagt ctcaagagct tcttggatgg
    12181 cttcattata tttttccttt gatactaggt agtgagcgta attgtaacga actctgatgt
    12241 agccaaataa aaactcttga tgctcaaaat tttttgtctg atataactcc atcaaatgag
    12301 agtagtttac ctcatattct tgttcacgac ccactaagga atagaaatta gatagagtat
    12361 tcaacgcctt taaataaatc aaagtatttg aggagacttt taataatata ttttccaatg
    12421 aagaaattgc ttcacactta ctgtcatatt gacagaagtc aataatagct ttaatccatt
    12481 caaggtaagt tcggtcttct agtgttagaa aagtacttcg ttcaatctct attctataaa
    12541 tatattccaa attttcataa tttctatcat ctaataggcg agcagataat tgcttgaaat
    12601 tagagaggtt agatttaatt tcaatttgtt cattaaaaaa ataatctaat ggcacttcga
    12661 gtcgttgtga gagtttgaac aaaaggtctg cggagggaat gaaataccct ctctcaattt
    12721 tactaatctg gctctattca caaattcctt ctgcaagagt ttgttgggag agtctcaact
    12781 cttttctttt caatctgaat ttctctgcga gtgtattcat taaagataat aaaccttcct
    12841 atttgcttaa tttcattata aaatttttag ttcaaaatag caagttaaag gaataaataa
    12901 ttctttgcgg taataaacaa aaaaaagtca aaaattacat aaaaatgctt ggctaaataa
    12961 aatatatttg ttaaaataag gataatgaaa aagaaaaggg tttcacgaat gaagaaaaat
    13021 cgtggaattc aaaaattagc tatattagta ttactaggtg tttttatgtt tagtaacaca
    13081 attccctacc aacagtttat tcagaagaat agacaattgg agattcgagt ccaatcgcaa
    13141 aagaagtcca atggtcttga tgttgggaag gctgattgat tttatattac tttaaaattc
    13201 aactatttag agaaaggtat tctgatgaga gcttgtaaca ataccagata gatataagaa
    13261 tggagatgtt tgataaaatg ctttcatagc taaccaactg ttgaaaatta tgagttacaa
    13321 aatctaaagt atttaagatt ggtttataaa attaggtctc tgcctgtaga aaggaacgtg
    13381 cagttagaaa ggaggaaaaa ataggatact ataattcaga ctgagatttc taattaaaat
    13441 atatagttgc atttcgctca ttttcataat atttcattca gaggagtaat aatatgttaa
    13501 aatttagaaa agtagttgca tcatcccttg ccgttttggc atttagtttg gctcttcctg
    13561 ttttggcagt ttctcaccgt ggtggcgagt ggacctatgg aggccatcat gatccaaata
    13621 attggggagc attttcgaat tattatcatg gttcacagta tcactgggcc tatgttggaa
    13681 gtaatggacg taacaatcaa aaaacagttt atgcaggagc acgtagtgcg gcctatgcgt
    13741 ttgttaatac aaattttggg gagcaagtta catttgatgc gggatggtaa agaataattg
    13801 aagaagattc ttttatcaat agttcagtaa gcttgttagt ttatagaggg caggggtatt
    13861 tctgccctct gttttagaat aaggaatgca ctatgaaaaa attatttata ctcatatcca
    13921 acctgcttgc tagtttgttc tttgtttggg tatttaccat ttggactgat acatatgtct
    13981 cctattatta ccccaacgtt gttgtacatg attcttcccc tgaaacaact tttcagcatg
    14041 ttgctacacg cttggaaaaa ctagcagaag aaacagatag ctttattgcg attcaacacc
    14101 aagatcctaa ctcagaaggt actacagttt tttcttatac gacctttggg gacggaaagt
    14161 tgcctgatgg gcttcaggaa aaaaaactag aagatgctca aagtagtagt gttgaaacaa
    14221 actattttgt attcgatgga cacctagata ttcacttgct aagggaggag ctaagtcaac
    14281 ttggcttgac taacatgaac ctgacaattc catctaaact atctacgtta atggctatct
    14341 ttagtaacgg atttcagtta atcagtctat tgattttcat cttaaccttt atagcactga
    14401 ctttaattag tcaaattagc caattacggt cgtcgggcat tcgcctcatt tcaggagaga
    14461 aacggtggtc cattttcctt agaccagttg gtgaggattt gaaagggata gctgttggtt
    14521 tcagtttagc tggcgtgctc gccattctta tgcaaaaaat tctgtcgctt ccaacgcaat
    14581 ccctaatgac cataggagca ggcttgctca gttataatct catcttgttg tcgatctccc
    14641 tgtttttcgc tcaactcttc gcagttggga taaaaaagat acacctgatg cagattataa
    14701 aagggcaagt gcctgtcaga ggaatcatta gtttgatttt aatcggtcaa ctattagcga
    14761 ttattattgt aacgctggga atagggagta gcttaaagta ttcccaagcc tggcaacagc
    14821 atcggattgg acaagaggct tggagtcagg aaaggcaact gattacccta tcaatcagcc
    14881 gtgaaggaac gagtcctggt tttgatgaac aagctcaaag gaaactcaga acttggtatc
    14941 aattgatgga tctggctgtt tcagaacaaa aggctttctt atctagacac cagttaattg
    15001 accgtacttt gcaaaatggc atggcttcct ccaaaaactt gataacctct acagaatggc
    15061 acgattacaa cccgaatggc aatgtcctta tcgtcacacc gcaatacttg gagcgtcaaa
    15121 acattcctgt agatacaact attgagcaaa agatgaatca ccttgatgta ggggagtttg
    15181 tcttattgct gcctgaacac ctccgttcag aggaagaaca ttataaatct gtttttgaag
    15241 acgacttaac cagtcgcatg tctagtcaag atgaacgaca gcaaatgact gctacggtag
    15301 gttatttaga atcaggtcag gatcgttttg tgtataatac gacccctatt tcttaccagc
    15361 agtttttgaa agatccaatc atcattgtta taacacccca atcaactggt ccacagtcca
    15421 ttttgttttg gatagacgca gtacagaact acgttctctt taatcaattg tctgatgccc
    15481 aggagcttat ccagagacaa ggcattgaaa attgggtctc agaaatgcaa acaggttacc
    15541 acaactacat cacattattg gataatattc agagggaacg ttgggtaatg ctagcaggag
    15601 ctgggcttgg gattgcaact tcaatcttgt tgtttaacac tatgaatagg ctctactttg
    15661 aagaatttag acgtgccatt tttatcaaac gcattgcagg tctcaggttc ttagaaatcc
    15721 atcgcactta tctctttgct caactgggtg tgtttttact gggatttgtt gcgagtgtat
    15781 ttcttcaggt agagatagga gttgctttct tagtcttgtt actctttact ggtctatctc
    15841 ttttacagtt acatgtccaa atgcagaaag aaaacaagat gtccatgctt gttttgaagg
    15901 gaggttaata tgattgaact taaacaggtg agtaaatctt ttggagaacg agagttattt
    15961 tcgaatcttt caatgacatt tgaggctgga aaagtctatg ccttaattgg ttcaagtggt
    16021 agcggaaaaa caaccttgat gaacatgatt gggaaattag aaccttatga tgggacgatt
    16081 ttttaccgag gtaaagactt ggccaattat aaatcgagtg attttttccg tcacgaattg
    16141 ggctacctct tccagaactt tggcttaatt gaaaaccaaa gtattgaaga aaaccttaag
    16201 ctaggtctca ttggtcaaaa gttgagtcgg tcggaacagc ggttgaggca gaagcaggct
    16261 ttagaacagg tcggcctgac ttatcttgac ctagataagc gcatctttga gttatcgggc
    16321 ggagaatcgc aacgggttgc cttggcaaaa gttatcttaa agaatccacc ctttattctg
    16381 gcagatgagc caacagcttc aatagaccca gcaacttctc agttgattat ggagattctg
    16441 ctatctcttc gagatgataa taggctaatc attatcgcaa cacataatcc agcaatttgg
    16501 gagatggctg atgaagtgtt cacgatggat cgtctgaaat aaaaatcctt gtttttaatt
    16561 gcacgatgag ttactgaaat attatcataa atcaataatt ggagttaatt tagaattgta
    16621 ctttattaat attgaggtaa ctttttcttg ataaaggaag aaatagtgga gaggaagtta
    16681 gaatgaaaaa attcgacaat tatattgttg agaagccttg cgattctaat tcagataaac
    16741 tgcaaaaaat cttaataatt gaaaatttgg tagatgatat tttgcaattt cctctcagaa
    16801 tcaataatag tgtaggagag attttcctcc tacaaccgtt tcaaaagaaa actatcttta
    16861 ttccatgtta ttttgaggaa gatattgtga aagtcaaaga tgatgataaa gttgagtgga
    16921 atttgttaga atttcaaaaa tttagagcat ttttggctta gtaatctgtg ttgaaggctc
    16981 aaaacctatg gtaaaaaagt cgctttgaaa acgtattgcc tccaaagatt tagttaaata
    17041 atgatttaac acaaaaagaa attattgaag ttctggaaag atgttgtttc agtattgaga
    17101 aaaggtggga aaaacttgcg attttcacag agaaaggaag aaaaagtata gaaatatagt
    17161 caattgaaac aagaacagga caaaagaacc ttttgtgcca tatttttctc ctttcgcttt
    17221 acaattggct tgaacacctt tattgtatcg cgtttggagt ttttttggta taaccttcga
    17281 cgcgcacccg catagcgggt gtttttttgt ctcgcaccta acggagcgag acgaactaat
    17341 agtcacttaa tcaaaaaacg caccatatca aaaactaaaa agtttgatat catgcgtcat
    17401 gtcttaaact aattgactat actttctatt caaatgagct tttaaccaat tgattgagcc
    17461 aatccactct taaaaccaaa gagcaatttc tcgcttagct gactcttctg aatctgaacc
    17521 atgtacaaca ttttggataa cctcattttc tccagcagct tttgcaaaat cacctcgaat
    17581 agtgcctggt aaagcttctt ctggacgagt tgcacccatc atggtccgcc aagtttcgat
    17641 tactttggga ccagaaatga cacccacaag aactggacct gaagtcatga attcacgaat
    17701 cggtgggtaa aaactctgac caaccaagtc ctgatagtgc tggtcaatca actcttctga
    17761 aacttgtgaa cgaaactcca atttttcgat tgtaaatcca cgttgttcga tgcgctttaa
    17821 cacttcaccc actagccctc tttttacacc atctggtttg atgataaaga atgtttgttc
    17881 catacccgtc tcctttgtca gcttctttct tttattttac cacatttcgt ggaaaaatgg
    17941 agaaagtttt cagaatagag aatgagagaa cccgagggtt ctctcattct ctattattct
    18001 actgtttctt ccacagtttc aacggcagta tccacaacta cttctgttgt ttcttcattt
    18061 ccttcttcct ctactggagg attaaggtat tcttcttcgt tgacagcata tggttcaagg
    18121 ttacggtaac gggccatacc agtaccagct gggatgatct taccgatgat aacattttct
    18181 ttaagtccaa ggagatggtc tttcttacca cggatagctg cgtcagtaag gacacgagtt
    18241 gtttcctgga aggaagccgc tgacaagaaa ctgtttgttt caagtgaggc tttggtaatt
    18301 cccataagga ctgggcgacc tgtcgctgga actccacctg cgataaggac atctttgttg
    18361 gcatctgtaa agtcattgat atccatgagg gtacccatga gaagatctgt atcacctgga
    18421 tccatgacac ggactttacg gatcatttga cgaaccatta cctcgatgtg tttgtcaccg
    18481 atttctaccc cttggctacg gtaaactttt tgtacttcac cgagaaggta cgtttcaact
    18541 gacaagacat cacgaactgc aaggagacgt tttggttgga tagaaccttc tgtcagagca
    18601 gcaccacgcg ctacttggcc cccaacttcg acacgcatac gagctgtaaa tggaacgaca
    18661 tattcacctt cgccagtttc acccttaaca aagactttct tggtacgagt tgatgcatct
    18721 tcttcgatag cagtaacttc tcctttaacc tctgtgataa ccgcttcccc tttaggattg
    18781 cgggcttcaa agatttcttg gacacgagga agaccctgag tgatatcggt atttgaggca
    18841 accccacctg tgtggaaggt acgcattgta agctgtgtac caggttcccc gatagattgg
    18901 gcagcaatgg taccaactgc ttcaccaact tcaaccgcat caccagtcgc caagttgata
    18961 ccgtaacagt gacggcagac accgtgacga gtgttacatg taaatacaga acggatagtc
    19021 acttcttcca caccagcatt gacaatttca cgcgccttgt cttctgtaat caattcattt
    19081 ggaccaataa tcactgcacc agtttctgga tgtttaacag ttttcttagt gtaacgaccg
    19141 ttgagacgct cttcgagaga ctcgatcatc tcttttcctt ctgcgataga acggatcaag
    19201 agaccacggt cagttccaca gtcgtcctca cggatgataa cgtcttgggc aacgtcgacc
    19261 aaacgacgag tcaagtaacc tgagtcggct gtcttaaggg ccgtatcggt cataccttta
    19321 cgagcaccgt gagttgagaa gaacatttcc aataccgaca aaccttcgcg gaagtttgaa
    19381 aggattggca attccatgat acgtccattc ggagcagcca tcagaccacg cataccggca
    19441 agctgtgaga agtttgagat gttaccacgg gctccagagt ccatcatcat aacgattggg
    19501 ttcttaggat cttggttagc aatcaagcgt ttctcaagtt tttcacgggc agcacgccat
    19561 tcagctgtaa cagcattgta acgctcgtcg tctgtgatca taccacgacg gaattgtttg
    19621 gtgatttgtt cgacacgttt gtgtgattct tcaatgattt cagtcttgtc atcaacgact
    19681 gggatatcgg caatacccac tgtcaatcct gcaagagttg agtggtggta accgaggttc
    19741 ttcatgcggt caagtagggc agaagtttct gtcgtacgga aacgtttgaa gatttcagcg
    19801 atgatatttc caaggttttt cttcttgaat ggagggttga gctcaagatt gctgatagct
    19861 tccttgatat ctccaccaag tggcaagaag tatttagctg gaacaccttc tgtcaagttg
    19921 gcattgtttg gttcttgcaa gtatggtagc ccctctggca tgatatcgtt gaagagaatt
    19981 ttaccaactg ttgtaagcaa gaccttatgt ctttgctctt ctgtccaagg cttgttgagg
    20041 ctgtctgttg cgataccaac acgtgagtgg aggtgaacat aaccattgcg gtaagccata
    20101 accgcttcgt cacggtcttt gaagaccatt ccttcacctt cgcgaccagc ttcttccatg
    20161 gtcaagtagt agttacccaa aaccatgtcc tgagatggag taactaccgg tttcccatct
    20221 ttcgggttca agatgtgctc agcagctagc atgaggatac gagcttctgc ttgtgcttct
    20281 tctgaaagtg gtacgtggat ggccatttgg tccccgtcaa agtcggcatt gtaggcttca
    20341 cagacaagtg ggtgcaagcg aagagcctta ccatcaatta agactggctc gaaggcttgg
    20401 atacccaaac ggtgaagggt cggtgcgcgg ttcaaaagca ctgggtgttc tttaatcact
    20461 tcttcaagga tatcccagat acgctcatct ccgcgttcca ccaagcgttt agctgctttg
    20521 acgttttgca cgatatcacg ggcaacgatt tcacgcatga caaatggttt aaagagttca
    20581 atcgccattt cacgtggcac accacattgg tacatcttaa gagttggacc aacggcgata
    20641 acggaacgtc ctgagaagtc aacacgttta ccgagcaagt tttgacggaa gcgtccttgt
    20701 ttacctttaa gcatgtggct caatgatttc aatggacggc tacctggtcc tgtgattgga
    20761 cgaccacgac gaccattgtc aatcaaagcg tcaactgctt cttgaagcat acgtttctca
    20821 ttttgaacga tgatacctgg tgcatttaac tcaagcaaac gagccaaacg gttgttacgg
    20881 ttgataacac ggcggtaaag gtcattcaag tcagatgagg caaaacggcc accatccaac
    20941 tgcaacattg gacgaagatc tggtgggata accggaagga tgttaagaat catccattca
    21001 ggtttgtttc cagacttgta aaaggcatcc aaaacatcca aacgacggat ggctttgaca
    21061 cgtttttgtc cagtagctgt tttcaattct tctttgagtt cagcaatttc tttttcaaga
    21121 tctacttgct tcaaaaggtc ttggatggct tccgcaccca tcttggcaac aaatgaacca
    21181 taaccatatt cgcgcaagcg ctctcggtat tcgcgctctg tcatgataga cttgtgctca
    21241 agtggtgtat ccttaggatc aatcaccaca taagccgcaa agtagataac ttcctcgagg
    21301 gcacgagggc tcatatcaag ggtcaagccc atacggcttg gaatcccctt gaagtaccag
    21361 atgtgagata caggagcttt caattcgata tgtcccatac gctcacgacg aactttcgta
    21421 cgcgttactt caaccccaca gcggtcacaa acaattcctc tgtaacgaat gcgtttgtac
    21481 ttaccacaag cacattccca gtcttttgta ggaccaaaga tcacttcatc aaagagtcct
    21541 tcacgttctg gtttcaaggt acgataattg attgtttcag gttttttgac ttctccataa
    21601 gaccatgaac ggactttact tggagaagct agggtgattt gcatactttt aaaacgattt
    21661 acatcaacca ctatttcttc cctttctatt ctaagtgaac tgcttattcc tgttcagcag
    21721 cttcttctgt tgcttccgct tttgttgctt tctcagcttc ttcagcttca aaggctgctt
    21781 tagcctcttg ggctgctttt tcgcgggctt tttcaaggtc atctacgtgg atgacatctt
    21841 cgtccattcc ttcatccaag tcgcgaagtt ccacttcttg gtcatcttcg tctaggacac
    21901 gcatgtcaag accaagagat tgcaattctt tgacaagaac tcggaaggat tctggaacac
    21961 ctggttttgg aattggtttg ccttttgtaa tagcttcata ggctttcaaa cgtccgttga
    22021 tatcgtcaga cttgtaagtc aagatttctt gaaggacatt tgacgcaccg taggcttcaa
    22081 gagcccaaac ctccatctca ccgaaacgtt gtccaccaaa ctgagcttta cctccgagtg
    22141 gttgttgggt aacagttgag taaggtccga ctgaacgcgc atgcaattta tcgtcaacca
    22201 tgtggtggag tttgatcatg tacatgactc caacagaaac acggttatca aatggttcac
    22261 ctgtacgtcc atcgtaaagg attgtcttgg catcgctatc catacctgct tctttaacag
    22321 ttgaccaaag atcttcagaa cttgctccat caaagactgg tgtcgcaatg tgaataccaa
    22381 gagtacgagc tgccatacca aggtgaagct ccataacctg accgatattc atacgtgatg
    22441 gcaccccaag tgggttcaac atgatgtcga ctggagttcc gtctggaagg taaggcatgt
    22501 cttctacagg aacgatacga gagacaaccc ctttgtttcc gtgacgtccg gccattttat
    22561 ctccgacctt aatcttacgt ttttgagcga tgtaaacacg aaccaacatg ttaacacctg
    22621 attgcaactc atctccattt acacgtgtaa agatcttaac atcacgaacg acaccatcgg
    22681 caccgtgtgg tacacgaaga gaagtatcac gcacttcacg agacttgtct ccaaagatag
    22741 cgtgcaagag acgttcttca gctgaaagat ctttctcacc cttaggtgtt actttaccta
    22801 caagaatatc accttcttta acctcagcac caatacggat aatccccatt tcgtcaaggt
    22861 ctttgagggc atcttcacca acgtttggaa tttcgcgagt gatttcttca ggcccaagct
    22921 ttgtatcgcg cgtttctgat tcgtattctt caaggtgaac agatgtgtag acatcgtcct
    22981 tcaccaagcg ttcgctcatg ataacggcat cctcgaagtt gtagccttcc caagtcatgt
    23041 aggcaacgat tgggttttgt ccaagcgcca tttctccatt ttccatagaa ggtccgtcag
    23101 cgatgaaatc gcctttttca acgacatcac caacttttac aagagtgcgt tggttgtaag
    23161 cagtacctga gtttgaacga cggaattttt ggatgtggta aacatccaat gaaccatctt
    23221 cacgacgaac ttctaccttg tcagcatctg cgtaagtaac tttaccatca tactgagcaa
    23281 tcacagccgc accagaatcg tgggctgctt ggtattccat accagtacca acgtaaggtg
    23341 cctgaggatt aatcaatggc acagcctgac gttgcatatt ggctcccatg agggcacggt
    23401 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acctgttttg
    23461 gtgaaacgtc catgtagtca acaatattag ctggatactc ttggttgacc ccttggtgac
    23521 gtcccatgac aatcttctca gcaaaggttc catcttcttt cagacgagag ttagcctgag
    23581 ctacagtata ttcatcttct tcatcagctg tcaaccaaac aatttcgttc gtgacaacac
    23641 ctgtttcacg gtcaacctta cggtatggtg tttgaacaaa accatatttg ttcaagtgtc
    23701 cgtaagatga caagttattg atcaaaccga tgttaggtcc ttcaggtgtc tcgattggac
    23761 acatacgacc atagtgagtg tagtgcacgt cacgtacttc atatccggca cggtcacgag
    23821 tcaaaccacc aggtcctaag gctgacaaac ggcgtttgtg agacaactca gaaagcgggt
    23881 tgtgttggtc catgaactgt gacaactgtg atgaaccaaa gaattcttta actgcagctg
    23941 ttacaggacg gatattgata atttgttgtg gtgtcaagac ttcattgtcc tgaacagaca
    24001 tacgttcacg gacattacgt tccatacgag aaagtcccaa acgtacttgg ttggcaagca
    24061 attcaccaac cgcacggata cgacgatttc caaggtggtc gatatcatct acacggccaa
    24121 gtccttcagc caagttgagg aagtagctca tctcagcaag gatatctgca ggagtcaccg
    24181 tacgaacctt gtcatctggg ttagcattac caatgatcgt tacgacgcga tctggatcag
    24241 ttggagcaac aaccttgaat ttttgaagaa caacaggctc agtcacaacg gctgcatcgt
    24301 ttgggatgta gacaatcttg ttcaagtcgc catccaaatg gctttcaatg ctttcaatca
    24361 cgctacgagt cataatcgta ccagcttcta ccaagatttc tccagtttca gggtctacca
    24421 atggctctgc aatggtttgg ttgagcaaac gtgttttaac attgagtttt ttattgattt
    24481 tgtaacgacc aactgctgcc aagtcatagc gacgtgggtc aaagaagcga gccacaagca
    24541 agctacgtga gctttcagct gtcttaggct cacctggacg aaggcgttcg taaatttctt
    24601 tcaaggcttc gtctgtacga gagtccattg gattcttgtg gatatctttt tcaacagtgt
    24661 tgcgaaccaa ttcgctgtca ccaaagatat caaagatttc atcatcacct gagaaaccaa
    24721 gagcacgaac caaggttgta aatggaatct tacgagtacg gtcgatacga gtgtaggtga
    24781 tatcttttga gtcgctttca agttccaacc aagctccacg gttagggata acagttgaac
    24841 catagcccac cttaccattt ttgtctactt tgtcgttaaa gtaaacacct ggtgagcgga
    24901 ccaactgaga aacgataata cgttcaccac cattgatgat gaaagtaccc atttctgtca
    24961 tgattgggaa atcaccaaag aaaacttctt gggtcttgat ttcgcctgtt tctttattga
    25021 tcaagcggaa ggttacaaaa attggtgctg agtagctagc atcgtggata cgagcttctt
    25081 ctagcgtgta ttttggttcc ttgatttcat atccaacaaa ttccaactcc attgtgtctg
    25141 tgaagtttga aattggcaat acatcttcaa acacttcctt aagaccgtgg tctaggaaag
    25201 ctttgaatga gtcagtttga atttcaatca aatttggtaa gtcaagaact tctttgattc
    25261 ttgaaaaact acgacgggta cgatgtttcc cgtattgaac gtcatgtcct gccaagatga
    25321 ttctcctttg taaataagtt ccaagccttg tcaatcaggc ttttctaatc gccatatggt
    25381 tgtaaacccc ttatcaccgt gtcctcttga cgaattttca gaatctttaa gcctctgtta
    25441 caaatgctca aaatcttgaa aaaaagcaca aaaagagcag ctaaatctga ctttttcaga
    25501 agatttaact gctgtgagcc ttgtctggac aatatttcag acaaaaccta cgacaaatga
    25561 ttacccatat tataccctat ttagctagat ttttcaaggg gattcagtag gtttttggta
    25621 atttttttcc catagaaaac ttggcatcac attcgaatca cgctatggta caaaaaactg
    25681 aaaaaactat tgactgaaaa tcattttcaa ggtataataa taaacgttaa ggcggtatag
    25741 ccaagtggta aggcacggct ctgcaaaagc ttgatcgtcg gttcaaatcc gtctaccgcc
    25801 ttctataact tgatttatca ggtttcaaat gaacagaaag cccaatttga agggcttttt
    25861 ttattttccc tcgaataaat acgtataact ttaaaaactt ttggagcgag tttgtggcag
    25921 agttctttcc atggcataat tcccttttga aatcaattga aaaaagcttc gtcacgcctg
    25981 gtgacaaaag cctctagttt attctgtttc ttcttctatt tcgacttctg tgatttggac
    26041 ttttaccttg gtaacacgtc cattcttcac tttatcattg gttaagataa actgtttgtt
    26101 ttggctgacc aattcatagc tgagtttctc agtcgttgga atggttccca ctcctgtcaa
    26161 ataataacca gcgatagtat caacgtcatc actttccagt tcaacattaa agtaattatt
    26221 gaaatcattg agggtcatag ttccttgaac aatgtaggta tcctcgccaa tctgatgaac
    26281 atctattgct gctttgtccg tttcatcatc aatttcacca acaatttcct ctaagaggtc
    26341 ttccaaagtc accaaaccag ccataccacc gtattcatcc agcaagattg ccatttgtct
    26401 ttgggtattt cgcagttctt ttagcaagtc atccacaaaa atagtttcag gtacaaaaag
    26461 tggatcttgt aaaattctct tccaaacaat attgtcaaaa ccgtccacaa agcctgcctt
    26521 aaggagactc ttggtgtgaa tgattccaat tacattgtcc ttatccccat cataaaccgg
    26581 gatacgagaa taattttgtt ttaaaatact ttggataatg gcttgactat catcctgaat
    26641 atccaccata aaggcatccg ttcgaggaac cataacctct cgtgccatca gttcatcgag
    26701 cgaaaagaca ccttgtaaca tctcaatctc atcagcatcc aatgtttctt cactatttgt
    26761 cagcatatag gcaatttcat cacgggtcat cttttcatcc gcatcatcga atgacatagg
    26821 agtcaaatgg ctcaagaaat tggtcgaagc agctaaaagc caaacaaaag gactgactag
    26881 ttttccgatc ccaatgataa ccggcgctgt acgaattgcc aaggcatcct ttagattaag
    26941 agcgattctc ttaggatata attccccaaa aacgatggaa atataggtca aaaatgccaa
    27001 ggatagaaaa gttgccacgg cttgtgctgt ttcgccattc ccaagccaag aggcaatcac
    27061 acgtcctaga gtatcagtta aactcgcccc tgataagatt gtaatcaggg tgattcctac
    27121 ctgaatggtt gataaaaagt ggttaggatt ttctagtacc ttcagcaggc ggatgtagcg
    27181 tctgtctcct tcttccgcct tttgttcaac tcgggcacga ttaagagaaa ccatggccat
    27241 ttctgtggct gagaaaaagg catttaatac cgtcaagata aacaataaaa caaattgtag
    27301 caacaaattc tgactacttg ggtcttccat agttcttctc ctaaaatagt atcttatcct
    27361 ttattatatc acaaaattta atccaataca tattattttt ttcttagcgt aacttccatc
    27421 tctctcccaa aactggaagt tagtctaaga cggatttcca ctgatttcac gcgtttttct
    27481 catactaaaa gctaggttgt gggaaactgg aagttagttt tagaagttac tattttttct
    27541 tagtacctgt tctagccctg cttaaactag taaaaagaac tcctacatcc ataagtgaca
    27601 taggagttta ggcttatctt ttactgagtt acagttactt ctccagttcg gtaatccagt
    27661 tgaagtccct tttgaacatt gggaactaga acattgaatt ccaattctcc atccgaagac
    27721 ttggcttcaa tctgtgaagc tgagggaact aaatcctcgt ttgaagcgta gtaaagggtt
    27781 acacggtaac ggacacgctt gttttggtcc aaggctttac gcaccttgct ttcatagtag
    27841 ttttgaccag tcgaatactc ggcttgtgcc tgatttgccc aggctgtctg aacagcaatg
    27901 tttttaggat tgcttgttga ggcatcaaaa ccatccaaac caccgattaa ggcatagcct
    27961 aacaaatgac ctctatcgac tgcatgggta taagagccct ttagattctt gacctgatgc
    28021 caacctggag gagtccaaga agttgaacca ttcccagttt ctttacgatt cttgtactga
    28081 cgagtggcct tagacaagag ggcattagct acggttggaa cagtttcctt acccactgtc
    28141 tttgttttat tgtcagcgta gggcttactt gaaaccttgg catctagatt tgttttatta
    28201 ccattgacga taaaagcacc tgagccattc cactccagac tcccctttat ttgactcttg
    28261 actgcgtctg ttaagacact ctctgccaat gcttgactag gagcttcagg cgcttgtttt
    28321 ttctgactaa gcttggtttt aggactatta gctgtcgact gcatctgctt gatataatag
    28381 ctccctgtag acaaaagcaa taacactagc agtccgatta gtgtctgtct tgtttttttg
    28441 ttcatatttc tccttatctt tagaaaaagg ctggttctcc agcctaattt cctgtaaatt
    28501 tatgaatcaa ttcctgccat tttgctggag acaggatagc ccatgaatct tgacccttgc
    28561 ccaagattcc ataacctacc attaaaccga ttcctagagc cagagtacct aaaatcagta
    28621 ctatgatgac taaaagtaaa cgcttgacta cgtagcttga tttcttattc atcttcatca
    28681 cttgcctcaa tccgctgaat cttagcacct agctgcgcca acttctcatg gaaaccgtag
    28741 taacctctat ccaagtgaac caatttaccg accacagttt ctccctgtgc taccaaacct
    28801 gtcaaaatca aagctgcact ggcacgaagg tcagttgaaa gaacttctgc tccctgcaaa
    28861 ggctgtccac caacaatacg agctgtatca cggataatct cagaatgcaa gcccatgcgg
    28921 cgcatctctt ctaggtgttg gaaacgattt tcaaaaactg tctccaccat ggttgattcg
    28981 ccttttgcaa ctgtcatcaa ggctgtaaat tgagcctgca tatctgttgg aaatcctggg
    29041 tggggcaagg ttttcacatg aacagctttt agattttcca gttgagaacg aacacgaatt
    29101 ccttcgtctt cttcaattac ttcaacaccc atttcaagta acttggcaat caagggacgg
    29161 ttgtgctccc agacagcgtc tcgaatcaag acatcaccac cagtcatggc agcagctacc
    29221 ataaaggttc ctgcttcgat acggtcctgg actacattgt gagtcgtacc atgaagtttc
    29281 tcaacaccag taatggttat agtctctgta ccagcacctt tgaccttggc tcccatttca
    29341 ttaaggagaa tggctaagtc aacaatctca ggctcacgcg cagcattctc aatcactgtc
    29401 accccatcag ccagagtcgc tgccatcatc aagttctgcg ttgcaccaac acttggaaag
    29461 tccatataga tatgagcacc atgcaagcgt tctgcctttg cttcgatgta accagctgtc
    29521 tgactaatct taacccccat agcttccaga cctttcaaat gaagatcaat aggacggcta
    29581 ccaatcgtac aaccacctgg catggatacc ttggcatgac ccacacgggc aaggattggc
    29641 cctaatacaa cgatggaggc gcgcatctta ctgacatact tgtaaggggc ttcctcagtg
    29701 atgtcgccag tagcatccac cttgacaaga tgagcttcct catcaaagtc aaccttggca
    29761 ttcaaaccac caactacctg attcataata aagacatccg acaaaatcgg aacattctgc
    29821 aagacggtct ttccttcact tgctagaata gtcgctgcca acaagggtaa gactgcattt
    29881 tttgctccct cgatcgtcac gcttcctacc agacgattat cgccaccttg aaccacaatt
    29941 ttatccatac tcgtttcctt tactcttgat tttataataa tcctctaagc gcttcttgcc
    30001 acaaatgata caggctaata aagaaagaac tcaggatgta gcctatgaca atgctaaaaa
    30061 aggctactaa tacacgaact tttcttgtat tctcagctgt cacttttaaa accttttccc
    30121 atctaacaag atcctttaaa aggtaaaaac tcacataaat aaagagcata tgactactta
    30181 gagtgaataa taattgaacc atttgactat tataccatct tctctgtttg tgcgctagtt
    30241 tggaaacttc actcaaaaat tagggattgt ttttcaagta atcaatcgca tcttgtaggg
    30301 ttttaacggg cacgattttc atatccgtct tgattgtttt agccgcttct agggctgttt
    30361 ggtagttgtt tttcgcgtcc ggatgcgcct tttgttcttc ttcgctaaca gggttatcag
    30421 gagcaaagaa aatagcagca cctgccctag ccgaagctac aaccttctta tcaatacctc
    30481 caatgtctcc cacattacca tcgcggtcaa tggtacctgt accggcaaca atacgaccat
    30541 tacgaagatc tgggtgagct atttgagtat agatagctag actaaacatg agaccagcac
    30601 ttgggccgcc aataccagct gttgaaaagc taattgggac attgctgatt acctctgtac
    30661 gatcaatcaa gccgattcca atcccatttt ttccattttc caaggtgata atcttccctt
    30721 ctgcagactt ggtttgccca tcctcttcat aggtgacctt gacggaatcc cctaattttt
    30781 gagaactgac gtaatcaatc aagtctttgg aactatcaaa ggtctgatca ttgactgctg
    30841 tgactgtatc agagatattg agaatccctt taaaggttga attatccgtc acattcaaaa
    30901 cataaactcc aaagtacttg agttcgatat ccttaccagc tgtttttagt ccttgatact
    30961 tggccatatt ttgcgatgtt tgcatgtaga attgattgat tcgcataaat tcaacatcgg
    31021 aagaaccacc tgtagtctcc tgagcactac gaatatctgt aaaaggtgtc aaccaagcat
    31081 aaatcatatg agctaaagta gcatgttgaa caccaaccgt aacgaattga taggcaccag
    31141 cttccttatc ttctgtgtca tttactttaa ggacttggcg aatatcttcc gaaccacctg
    31201 gaacctctat ataataaggc aagggcacta caaatgccaa gaaagtcaca atcaaggccg
    31261 caatgacgta taagggccat ctaatctttt ttttcatttc ttatttcctc caaaatactc
    31321 tcgggaacat agcaggcaat atcctgacca aacttcaaaa gctctctaac gcctgatgaa
    31381 ctgatataga gatgttcagg tcgactatgt aaataaatag tctctatatc agaagacagc
    31441 tgatgattgt agtaatcaaa actggcttca tattgcaaat ccgacgcatt cctcaaacca
    31501 cgcactagac aagtagcacc caatcttttt gcaacgtcga ccaccaattc atcatgagaa
    31561 gccacgactt caacattttc caaatgtccc aaagcctttt ctagcccccg tttacgattt
    31621 tcaagaggga gaaatccttg tttgtgggga ttaaaaaaaa tacccacaca aagcttatca
    31681 aaaagtctgc tcgcccgttc aatgatatcc agatgcccat ttgtcatcgg atcaaatgag
    31741 cctgtgaata agccaatctt atctgacata gactgtcacc ttactaattc cataaatctt
    31801 ttccttccag atacccagac aggcaatttc ttctggaagt tcaacggtct tatccgtctc
    31861 gcacacaacc ataacatctt cagaaaaaag ctctctctca gccatttttt caatatctgc
    31921 tacgatttgt tccttggcat agggagggtc taagaaaacg aggtcaaatt ccccagatac
    31981 ctgttccaat gccctttctg catccatttt gaggagttga aattttccaa cttccttggt
    32041 catctggata ttttcagcca cgatggtctg agccttacgg tctcgctcca ccaaaacagc
    32101 actggacatg ccacgcgata ctgcttcgat agataaacca ccactacctg cataaaggtc
    32161 caagactcgt cccacttcaa agtagggacc aatcatgtta aaaatggctc ccctaacctt
    32221 atccgaagta ggtcttgttg tcttgccttc tagtgtcttg aggggacgtc ccccatagat
    32281 tcctgatacg attttcatac tgtttattat accaaattat agacaaaaag agaaagaaaa
    32341 ccgaaccttg cggttcgatt ctctacaaaa tattttcgta agtatcgcgg acttcttgag
    32401 gccaaacact tgtttgcact tctccgatgt gtctcttgcg aagtaggaac atggccatac
    32461 gagattgtcc aattcctcca ccgattgtca atgggaatag gccattcaac aaagacttgt
    32521 gccattccaa ttctaagcgg tcttcatcac ctgtaatttc cacctgacgt ctaagagttt
    32581 cttcatctac acgaattccc atagaagaca actcaaaggc tccacctaaa gactcattcc
    32641 agacaagaat atcaccattt agacccttgt agccattctc agactcgctt gtccagtcat
    32701 catagtctgg tgcacgtcca tcgtgcggtt taccatctgg caactcgcca ccgataccaa
    32761 tcaaaaagac ggctccaaat tctttacaaa tcgcattttc acgttcttta ggtgtcaagt
    32821 ctgggtagcg ttctaccaat tcttctgtat ggataaaggt gatttgtttt ggcaagatag
    32881 actcgatgtc atagcgggct tcaacagcta gctcagtcag gcgaatagcc ttataaatct
    32941 tctcaactgt ttcttttaga taaacgatgt tacgcttacc atttgggata accttctccc
    33001 agtcccactg gtcaacataa acagagtggg ttgcatccaa ggaatcctca tctggacgaa
    33061 gggctttcat gtggacaaag agaccctctc cttcaccaaa gccaaaacga gccaaggtgt
    33121 ggcgtttcca tttagcaagt gagtgcacca cttcataagt agcatcaggg atttggagaa
    33181 ccttgaccga tactggattt tccacaccag acaggttgtc ctgcattccg tcaccgacct
    33241 tactcaagat aggaccttga acttcgacaa cttctagctt atctttcaaa tactgggtaa
    33301 aagtgttttt gacaaaggaa atttcttctt gttgatggat aaaacttttc ttcatagaca
    33361 actcctcaaa aatttaataa acgtattata cacctatttc caagaaaaga aaagagaaaa
    33421 ttttaaaaaa atcagtgtca ctcaaaacac tgatttcata gaccttttag tcattgcgac
    33481 caaagatacg tagaatactt aggaaaagat tgataaaatc aagatagatg ctgagagcca
    33541 ttgacaccac ccagcctgtc gctacacgac cttgtgattg ttcataggcg aggcgaatct
    33601 tttggttatc ccaagcaatc aaccctgaaa aaaccaaaac catggctacg ctaatcatat
    33661 aatcaaagaa gccactagcc aagaaaatat tgactaccat agcaatcagt aaaccaatga
    33721 gagctgccat cattgctcga ccaatcccac tcaagtcttt cttggtaaaa ataccaactg
    33781 ccgccatgac aaagaagaga agggcgctcg atacaaaggc agataaaact gtacccggag
    33841 tatagaaagc caccacaaaa ctaagggtaa agccgtttaa aacggagtaa agtaaaaata
    33901 ctggaagagc agctggacta tttcttgaag ccatgctact ggcaacgaaa accaaagcca
    33961 actccgcaaa agtagcaatg gtcaaccaga gacgcccctg catcaacaag taaaccaact
    34021 gagactgaaa gaccgtcaac ataaggcctg ataccaaagc agatagtccg attcccagac
    34081 caacaaaggc ataaacctta gcgtaaaatt gattgagacc tgcacggtca tgtataatag
    34141 tgtgattcat ccttttctcc ttttctgtga acatctttcc ttgattataa caaaaaaagt
    34201 taaaattagc ttaaatggaa gattaaaata ccggctgcaa ctgcaacatt caaactctcg
    34261 gcctgcccct tcatgctaat atggaccaac tggtctgcac tttcagccat aaggggacta
    34321 attccttgac cctcatttcc cataactagt acaaaatttt ctatagaagg cagttctctg
    34381 taatcaacag aatctttaga tagggttgtt gctagcactg ggatagctac ctttttagtt
    34441 tcgtcaagaa gcgcttgact agtcatccgg taaatgggca gatggaaatg actaccttgc
    34501 atggaacgta gggttttgag actgtagatg tctgccgact tatctgaaac aatcactcct
    34561 gtaaaacctg ctgcatccgc agttcgaatg atagttccta cattaccagg atcttgcaca
    34621 tcttccaaaa acaagaactt gccctgactc aaatcagctt gtcctacttc ttctttttga
    34681 accacggcaa caattccctg tggagtctga gaatccgcca aatctagcaa aatatcctct
    34741 gaaatccaga cagtttgcgg aaaagcagct aactgatttc gataattttc tagggcaaag
    34801 attttctcaa tcgtcactcc agcttggaca gcttcttcaa acaagtgcca gccttcaatc
    34861 aaataggcag acttgcggta ttttttttgg tgtaatttct tggcattttt taccacagaa
    34921 ttggcttttg aggttataat agtcatagaa atattataac acaatcaaag gggtttggta
    34981 tgcaaaaggt tagaatgatt gcccaaggta gggtgcaggg agtcggcttt cgttggggtg
    35041 tttatagcct ggcccttgaa atcggtggca tcacaggtcg ggtatggaat aacgacgatg
    35101 gcacagtgga aatcttagcc caagcagact catctgctat catggcaaaa tttatccaag
    35161 aaatccgaaa aggaccgaca cctttttcaa aagtaagcta cttagatgtc aaactaagca
    35221 actttcctcc ctactctgac tttaaaatcg caaattaggt ctccagaact attgtatatt
    35281 ttgtaaaaaa acagtagaat agaaaggtat aatttttaaa gaaggaataa aaacagtgaa
    35341 atctattaaa cgttttgcat tctcggctat gggagtggct atgttgcttg tcttgactgg
    35401 ctgtgtcaat gtcgataaaa ccacaggtca gccaacagga tttatttgga atacgatcgg
    35461 agcgcctatg gctgaagcca tcaagtactt cgctactgat aaaggtctag gctttggtgt
    35521 cgctatcatt atcgtaacca ttatcgtacg cttgattatc ttaccacttg gtatctacca
    35581 atcatggaag gcaacgcttc actctgaaaa gatgaacgct ctcaagcatg tccttgagcc
    35641 acaccaaaca cgtctcaaag aagcaactac tcaagaagaa aaactcgaag cccaacaagc
    35701 cctctttgcc gctcaaaaag agcacggcat cagcatgttt ggtggtgtag gatgtttccc
    35761 tatcctcctt caaatgcctt tcttctctgc tatctacttt gctgcccaac atactgaagg
    35821 ggttgctcaa gcaagctacc taggcattcc tctaggttct ccaagtatga ttttggttgc
    35881 ctgtgctggt gtcctttact atcttcaatc gctcctttca cttcacggag tagaagatga
    35941 aatgcaaaga gaacaaatca agaaaatgat ttacatgagc ccactcatga tcgtcgtctt
    36001 ctccctcttc tcaccagcta gtgtcacact ttactgggtt gtcggtggtt tcatgatgat
    36061 tctccaacag tttatcgtca actatatcgt tcgtccaaaa cttcgcaaaa aagtccgtga
    36121 agaactagcc aagaacccac caaaagcaag tgctttctct aaaccaagtg gacgaaaaga
    36181 cgttacccct gaacaaccaa ctgctatcac aagcaagaaa aaacacaaaa atcgcaacgc
    36241 tggaaaacaa cgttcgagat aagaagaaaa aggcttagct gatttgctaa gtctttttgc
    36301 aaatcaaaag cccgatgttt ccatcaggct tcttttctat ccatgtacac gtttcatata
    36361 atcttgataa ctttcggtat ccatgagttt tttagcgttc ttgacgcgat ctgctgttgg
    36421 tggtttgact ccttcgaggg aatatggaat tccaagttca cgccacttga actcacccat
    36481 ggtgtgataa ggtagaattt caaacttatc aacatttttg agggtcttga cgaacttacc
    36541 aagttcaatc aagtcatcat ctctgtctgt caatcctgga actagcacgt ggcgaatcca
    36601 gacaggtttt ccaatatctg atagatactg ggcacaagcc aagatatttt tattagtttg
    36661 gctagtgaca atcttgtgct gttcttcgtt gatttccttg atatccaaaa gaaccaagtc
    36721 agtgacagcc atgagtttgt caaacttctc aaggtaacgt ggtttattac ggaaaggaag
    36781 agcacaggta tccaaggtac agtggattcc ttgttcctta gccttggtga agagagcaat
    36841 caggaaatca atctgcaaga gagcttctcc tccactgact gtaatcccac ccttatttcc
    36901 ccagaaacca cggtagcgca aggcctctgt caagacatca tctaccgtcc gttcacgtga
    36961 cttattggac tccatagccc aagtgtctgg gttgtggcaa tactggcaac gcatgtgaca
    37021 gccctgcaaa aagacaataa agcgaatacc aggcccatct actgacccaa agctttctgt
    37081 cgaatgcacc attcctgtca cttgtccata atcaattgtt tcttcagaca tatcgttacc
    37141 ttccttgaaa acgttttata gttttattat atcacgactt gaaggaaaaa caagattttt
    37201 gcctattttt tagaaagcaa tctgtttttc aatttcattt tcaatctatt atcagattat
    37261 tcttcaaaat cgaagccata taaggttgca aaatagtcct cagggcgctc tgcacggcgg
    37321 atttgacggg ctttaccttc ttcggtatag aggatttccg cagaacgtaa tttggcatta
    37381 tactggtagc ccattgaaaa tccgtgggca cctgtatcat gaattaccag caaatcaccg
    37441 atttctgtat gaggcagttc gcgattgact gcaaatttat cattgttttc acagagtgaa
    37501 ccaaccacat ctaccgcttc agctggtcca tctggatggg tcacgttgct aatatgatgg
    37561 taagctccgt acatagctgg acgcatgagg ttgactgctg aggcatccac acctagatag
    37621 gtacggtagg tttccttctt atgagtcact cttgtgacta gagcaccgtg aggtgccagc
    37681 ataaaacgac ccaattcggt gaaaatcttg acctgaccaa gacctgctga cgtaagaact
    37741 tcttcataca ccttacgaac tccctcacca atcaaggcga tatcgttcgg ctcctggtct
    37801 ggacgataat taacaccaat accgccagaa agattgataa agtctagcga aatgcccaac
    37861 ttttccttga tttcaacagc cagttcaaag agctgacgag ccaactctgg ataatagaga
    37921 tgggtcacgg tattggacgc taggaaggag tgaatcccaa aagtcttggc tcctttttcc
    37981 ttcaagatag caaaggcttc aaagagctgg tccttggtca tgccaaactt agcctcccca
    38041 ggattgtcca taatgtctgt ccccagttca aaaacgcctc caggattata acgacaagag
    38101 atgatttctg gaatgcctgc tactctctcc agatgttcaa tatcttcaaa ggcatccaag
    38161 ttaatggtcg cacccaattc acgtgcatag gcgtattcct tgtctggcgt gttgttggaa
    38221 gagaacataa tctcagaacc cagaaagtcc agtttatggc tcatcaaaag ctctacataa
    38281 ctagagcagt ccacaccaca accttcttct tggagaattt tcaaaatagc tggagttgga
    38341 gtagccttaa ctgcaaaata ttccttaaag cccttgttcc acgaaaaagc ttggttgacg
    38401 gctcttgcct tctcacgaat ccccttctca tcatacaagt gaaagggagt cgggaactcg
    38461 gcaacaatcg cttctaactc ttctctattg ataaatggtg ttttcataag cttccttcta
    38521 ttctgtttgc aaagaaaggc tgggacaatc gttccaacct ttagttctat ctcttattta
    38581 tcttcgtcaa agatattgcc aacctcaaca tcgatggctt ggttcttgac atcaatattg
    38641 gttaccttca agagtgactt gtagtcaaac tgcttttcac gttcttcttg ggcattgtcc
    38701 gcaaagaccg ctacacctgc cagttctgag tcgaactcgt gcaagagact aatcatacca
    38761 ttgaccgttc cgccaccttt caagaagtca tccacaatca agacacggct gcctgcctta
    38821 agactacgtt ttgaaaggaa cattttctcg atacggtcac cacttgaacc tgaaacatag
    38881 ttgacgctaa cagttgaacc ttcggtaatt ttcaggtcac ggcgcacaat gacaaaagag
    38941 acattgagga cattggcaac tgcatttgca agtggcacac ccttagttgc tacggtcata
    39001 accgcgtcaa ttttttggtc cataaagctt ttggcaataa tacgaccaat atttttcaag
    39061 atggctggtg tgctaagcaa atcagacaga tagatataac cacctggcaa gatacggtca
    39121 ctttctgaca acttggtacg caagtcttca accatttcct tagcatcctg actcgaaata
    39181 gacggtgtga agatgacacc tccgccagcc ccagtcactg tctggatatg accgatttca
    39241 atttcctcaa aggcgcgttt gataatgacg atatcttctg agatggatga tttagcagac
    39301 tcatactttt cagcaaaagt attgagacta gttagtttat aaggattatt aatcaaatag
    39361 ttggaaatga caaccatccg atcacttctt cttaatttca tttctatttc ctattccatt
    39421 tttattcaaa aaatcaaaaa gcaaactaga aagctggtcg ctggtggttc aaaacactgt
    39481 tttgagattg tcaatagaac tgacaaaccc tgtaatatac ctgcatatat acatacgaca
    39541 aggcgatact accctagttt gaagagattt tcgaagagta ttcatttttg tcttttactt
    39601 attatctttt acttattata ccatattcac ataaaaaaac gaacattctt atcctaaaaa
    39661 atgctcattt ttcttaaatt atcaatctaa atctggttta tagaaggaac gattatccat
    39721 agcgaagatt ttattggtca tctctccttt atccaccaaa gccagagctg ttgacatcat
    39781 catcatgctt gcatccagat tgtcaatcat atggataatc tctgcctcca taatacgtgg
    39841 acggactgga cttccatatt caagcaagcc gtggtgactg aggatgacat gacgaagcaa
    39901 aacgacttct tccttggtat catcgatgcc gagttccata actgtcttgg taatttcgct
    39961 atcaatgaga gcgatatgtc caagaagatt acctcgcact gtgtactctg tctggtctgg
    40021 ccccgtcaac tcgataacct tagctaagtc atgcagcata atccccgcat agagcaggct
    40081 cttattgagc tgaggataaa cttcgctaat agcgtctgcc aaacgtacca tggtcgccgt
    40141 atgataagcc aaccccgttt caaaggcatg gtggttggtc ttggcagctg gataggagta
    40201 gaattcctta tcatacttgg tgtagagatt tcggacaatc cgttgccaga caggattttc
    40261 aattttgaaa atcatttgcg acatgtagtc acgaatttcc ttgacatcaa ctggtgactt
    40321 gaccttgaaa tcggctgggt cattgggttc accagcttga ggcaggcgga gagtaatttg
    40381 attgacttga ggggtattgt tataaacttc tcggcgtcct ttcatgtgga caaccttacc
    40441 tgcggtaaag gcctcaatgt tatgaggttg ggcatcccag agcttcccat caatctcgcc
    40501 actatcatct tggaaggtaa aggctaggta gtttttccca gctcgagttt gcctcaggtc
    40561 agctgatttg attaggtaaa agccttcaaa tagctcatct tttttcatgt gactaatctt
    40621 catattcttc ctcattttct tgaaaatgga gtagatcaag cgcaggctca ccttctgaca
    40681 actcaatgtg acggagcgtc cgctcgatag ctatggtacg acggtttaat aattcatcaa
    40741 tattgccaga ggcatgttgg agatgttttt gtgccttgac cagaatacca ccaaacttgc
    40801 caaactcggt cttgacactg gcaagagtct tgctgatatg gtcggcactc ttttggatat
    40861 taagggtctt gaaaccaact gatagagagt taagaagggc tgatagggta cttggtcctg
    40921 caacaataat ctgttcttcc cgtctcaaat catcaaagaa gaccggattg cgcacaatct
    40981 ctgaataaag accttctgtc ggaacaaaca agattccaaa gttggttgtc cgaggtggtg
    41041 ctatgtactt gttcctaata tccctagcaa agcgcttgac gcttgctagg agtgacttac
    41101 gacagcgttc aatctcatcc ttgtcacctg tctcataggc ttcttccaag cggtaataat
    41161 ctgccagtgg aaacttagag tcaattggca gatagacgta ttcttggtcg ccttgtccgg
    41221 gtaacttgat ggcatactcc actcgttcac tagagttttc aaccgttgcg tattctcgtt
    41281 cgtactgggc aggtgtcatg atgtcttcaa taatttgccc cagttgcaat tctcccagaa
    41341 tccctcgcgt cttggttcca gagagaacct tgttaagagc tccgacatca cgggcaactg
    41401 tctgcatttc tccaaggcca cgattgacag actccagttg tttagaaact gtctcaaagg
    41461 aagcctgtaa gcgtgtctgc aaggtctttt ctagtttttc ctcgaccgtc tggcgcattt
    41521 gttccaaacg ttgctcattt gattcctgca aggcttggag acgttggtct gtcttgtctc
    41581 tagtttggag gagattatct gtcatttctt gacggacttg ggtcagacct tggtgcaatt
    41641 caatccgcac ttcttgcaaa cggtcgctga caaccacttc caaatctttt tggtctaact
    41701 ggctggcttg tctggcttgg tcaaagcggt aatccaactg gtctgacaaa tgatctgcct
    41761 gatcctccaa gctcttactt aagtgtttct cctgcctatc ctgcctttgc caaatcagaa
    41821 agagaccagc tagattggca attaataata gaataagtaa actttccatc ctacctcctg
    41881 tccttgctat gcagtacgac cacataacca tctgggcaag ttaccgacac ttccctatct
    41941 atatattcgt tagaagcgta cactttttta aagaaaaaat tttcctctgt caactcatac
    42001 ttggctccaa gaatagtcag ctggctatcc cgaactggca taaaggctag atagtcgtag
    42061 tctgaacgag gttctagctg actgattcct tctggacaat aagtaatcaa gttttgccca
    42121 tcctcaattt ctatttgatg catataggat gccaacttag gattgctagg cagaaagaca
    42181 ttggccaaca tatggtcaat acggccaccc aaggcaccga aaatagtaac ctgagcctga
    42241 ggattttgct taaagatggt caagagagcc aattccaaat cagtatcatc tttttctggc
    42301 cgggcttgga caaaatactg ggcacgtttt tgaatcacct gtcgctcttc ttccgtcaca
    42361 gaatcaaaat ctccgactgc tagagcaaga ggcaaatctt cttccaagat ccagagcgag
    42421 cctcgatcca caccaacaaa accatcaaag tccgtccgat aatgaccgcg gtctccgcct
    42481 gcaaaaacgg caaccctagt ccagttgttt tctgagagtt tgtactcgct cattgacatc
    42541 tcccttaaag acataggaac ctgctacaaa aacggtcgca ccagcttctt tggcttgagc
    42601 aatagtttgg tcatcaatcc caccatccac ttcaatctca aagttcaaac ctttttcatc
    42661 acgaagggca accagctcac gaaccttatc catggtttct ggcagaaagg cttgaccacc
    42721 aaatccaggg ttaactgtca tgactaggac ttggtcaact agatgaagca catgcttaat
    42781 cgcttcaact ggtgtaccag gattgataac aactgatggc ttgacaccga gtgaacgaat
    42841 cttttggagg gcaccatgaa tatgaggcgt tgcttccaca tggatactaa taatatccgc
    42901 acctgcacgc acaaagtcct ctaaatgatg ctcagggttt gacaccatca agtggcagtc
    42961 gaaaaccatc ttactatgag gacgaagagc ttcgaccaca cccgcaccaa aactgatttg
    43021 cggcacaaaa tgaccatcca taatatcgat atgggcatac tctgccccag ttgcttctag
    43081 gcgtttaatt tcacgttcaa agttggcata atctgctgcc agaattgacg gagcaatctt
    43141 gtattgagac ataggtttct ccttattttg gaattttttt gctgactttt ttataggttt
    43201 ctctgcgatt ttcaatttcg ctgaggaatt gcaggtagtt gtcaaaacgg aagggcgcaa
    43261 tggtaccttc ttcaactgct ggcttgacag cacaggacgg ctcatgggta tgggtacaag
    43321 tacggaattt gcagtctcga ctgacagtgg caatctctgg aaaggcctga ttgaggtctt
    43381 cagccgttga tacttcataa tccagtgatg aaaagcctgg tgtgtctgcg attttacccc
    43441 cattgaggtt gtaaaaacta acagctcgag tggtatgacg cccgcgaccc agactgtctg
    43501 aaatttctcc cgtttcaaga ttaagatctg gtgcgatttt attgagaaga gttgatttcc
    43561 caacacccgt ctgccccata aagaccgtaa ccttgcctgt taacaaaggc aggagttcct
    43621 ctttactggt cacaaagtca tatccgatgt caccataggt ctgctggtaa aaatccagtt
    43681 ctcccccgtc ttccaacaaa tccattttag aaatatagac gatgggatga atacccttat
    43741 gctccaaaag aaccaagaaa cgatccagca aattgctgtt aaaatcaggt tccttgacag
    43801 acatgattac tacagcttgg tcgatattga caataggcgg acggaccaga ctgtttttcc
    43861 gttcgtgaat cttgagaata tagccttctg aattttcctc ggcagagaaa tctacccagt
    43921 ccccaacgta aggggtatgc ccttttttac ggaaattccc acgcgcgcgt gtttgataga
    43981 cttggccatc actctctaca tagtagaacc cagccaaggc tttaatgatt tgtccctgca
    44041 aaataactcc tttaaaattg ttagttccat tataacaaaa aaaagctatt ttagcttagc
    44101 ttctctttac aatttcgata agtctattct aaaagaaaaa gatacactca cgctttagat
    44161 accaagtttt atacaaaaat aacgaatcta cctccttata taaaaacagg cgaacagaga
    44221 gcctaaaact ccctgttcgc tttacttttt ctgtgtgtaa tagaaaaaac ctaataaagc
    44281 aagacaaaaa atatgaatat aaactccata catcatgtgg ggaaagacag actctataaa
    44341 atagcctggt agatagatga ttggaaattg atagaaatca taggtcgtta gagatagtaa
    44401 acaaagaaca tacataaata ctgctacaat tagaacccat ccttgctttc gccatagaaa
    44461 aatcatcaat aagaacagac aaagtagata gattgaccaa tttcgcaaca taaattgaaa
    44521 accataaatc atactttcag tcgtttctcc atcaatcaca tcaactactt tatagaaagg
    44581 taaacaatag ctcactaaaa aaagaagggg actaattatt tttttcatat tctctaccat
    44641 ggattatttt gcaagtaagc tgaccatcta tctccatatc gaatcaaatt aacagtataa
    44701 ttagttacta cccgattttc acctggtcca accttagaaa aacctgtgct atactcccaa
    44761 ctacttcttg cacctggatg aaaccatttt tgctgggatg aataccaacc accttgtcta
    44821 acttccacac ttgaagattc tatttcgata tccctttcat ggaagtcaac tcctccactt
    44881 attttagtaa tatttttttg tacacctcca tcataactca cccagtgaac aacagtatac
    44941 aattccacac tataagtact atcaaactga ctttgatgag tagtaatgtt ttgagtataa
    45001 gcgtgcactt tctgtatacc aaaaatatgg tctaatatat tcgttggttt agcaagctta
    45061 ttgtaaccct cttcaacaaa agtagccttc tctaaatcag cggtataaac agtttttcct
    45121 tgtactactt gattgtttat agaagtaata cggctatcca caacaatggg ggccaccata
    45181 tctccatcgc tagtttctat tttagctgcc tctttcacct catatacaac tcgattttct
    45241 tcagactgaa tcacttctac cccatttttt tcaggtatag ataatctagt ttgcccatta
    45301 gaaaccgtta tatttggacc aatattcttc tcatttgcat aagtcgcaat aggaaatcct
    45361 gatagaacca ctgcacacat aaggctattg actaattttt ttctcatgaa tatatccttc
    45421 ccattttcaa gaggagtaaa aatagctaat ataagatgaa cgtttcctta tatttcagaa
    45481 taggatatct atctgaaagc aattcacact cctatagata actgttccaa atcttggcta
    45541 catatatttc aatagtttgc tacaaaaaac tcctttctaa gtctattgaa catgccctta
    45601 ttcacctact aatatatcac aaaaataaat aaatacaaga ctgttttttc tactagtttt
    45661 atgatagaat agtattgtta gggtaaattt catatataaa tgtcgagtga aaaatcgatg
    45721 gagcagaaat cggtagacaa agactcgatt tcgttggtcc agccccacaa ggctaatcag
    45781 gtatttcacg agtttaaaaa tctaatgaag tgcccaatca actactgatt cattgattta
    45841 atacgaacta tagtggattg aacctagaaa tagtacacta aggattctaa aacgtttcta
    45901 gaaattaatt tgactttcct aatctctttg ttcatatctt atttcaatct actatatata
    45961 ccaaaatgct aggacaaaag tctttaaaat cagagaaacg catattatca ggtgttggaa
    46021 aaccttgata atatgcgttt tatcatagaa agatctactt cattttttac taaaattaag
    46081 tttttgtccc tgcatcttag agtccttgcc ctttaagtgc atctgctagg ctggcaaact
    46141 cttccaagct gagagcttcc ccacgcacac ttggtgacaa gcctgcttgg tccaaagcct
    46201 tggtcagctt gtccttgacc tcttcagtct tgccaaagta acctgtcaag ttattccaca
    46261 aggtcttgcg gcgatgggta aaactagcct tggaaacctt aaagaaaaag ttctcgtctt
    46321 ccactgctac agctggcact ggacggcgca ccattttcaa gatggctgag tctacatttg
    46381 gtgctggcac aaagactgta cgcggcacga taaaggcaac cttggctgtc atgtaatact
    46441 gcacagcgat agacaagcta ccgtaagcct tggtatttgg ttgagccgag atacggtccg
    46501 ccacttcttt ttgcatcatg accacaaact cactaaaagg aatgccactc tcgattaagt
    46561 gcatgagaat aggcgttgtg atgtagtaag gcaaattagc cactaccttg attggcaggt
    46621 caggattttt gaaattctgg atatgttgcg ccaaatcgac cttgagaatg tcctcgttga
    46681 ctacagtcac attgtcaaaa tcacgcaagg tatctgccaa aatcggtacc aaacgatggt
    46741 cgatctcaaa ggccataact tctgctgcac gctcagccaa aaactcggtc aaggcaccaa
    46801 tacctggccc gatttcaatg acattgacct ggtcatcaat ctcagccgta tccacgattt
    46861 tttgtaggat attggtatcc gtcaagaaat tttgcccaaa ggacttttta aaggtaaaac
    46921 cgtgacgctc cagcactgcc ttggtcacgc tataatctgc aattctcatc tattctcttt
    46981 tctattatct gttactacta ttgtaacaca ttccccttgt attttggggg tgaaaatcat
    47041 accaaaaaac tatctttcct catgaagaaa gatagtcttg gaaatcttta aaagtggcct
    47101 tagcgcttct ctcgaaacat acgttcatac tcttcgaaaa tctcttcaaa ccacgtcagc
    47161 ttcaccttgc cgtactcaag tacagcctgc ggctagcttc ctagtttgct ctttgatttt
    47221 cattgagtat caaaaaaatc agcttcttga taacttcgat tttcgggtag ggtcaaatcc
    47281 acatcctcct ccacatcatc cactgtcagt tcaaccgttt gaggtaaatt tttcaaacga
    47341 taatgctttt gcctttgccc ttcactacta gcaaaatcaa gcaaatagcc atgcactcgg
    47401 atttgaacca aaaggagagg attggtcata ttttccatga tgaggatatc ttataaacca
    47461 cctctggcac taggataatc agataccctg cgaatacgga ttttttcaca catgagcaag
    47521 tttcctcata tcaatgattt catcagcctt attccagact aggggattat gggtcgcaat
    47581 gatgataatt cgattttcat ctttcaaatc caccaagaga ttcataacct cctctgaatt
    47641 ttcaggatca agagctgctg ttggttcatc tgccaaaatc aagggtggat ttttcaaaat
    47701 agttttcgca agggcaactc gttgggcctc tcccccagat aaagtataaa ttttttgttc
    47761 taaatccaaa taccctagat taactttttc taaagccccc acttgccttt ccaaacgttc
    47821 tacttttgag attttctgac caacaaaacc caaatccaaa ttttctttga ttgattggtt
    47881 ttctaagagg ccaaaatttt gaaagagata gcccatctga tctcgaaaat actcacgagt
    47941 gggaatggtt tttaaatctt tcccctgata gagaaccctt cctccatcta tcttttctag
    48001 ctttcccaag atattcagca gcgtcgtctt tccgcttcca ctctttccga ttaaggcata
    48061 aaccttgccc ttctccagct tgagattcaa accagagaaa atcgcgcggt cattaaattt
    48121 cttttccaat ccttgaatat caatcatctt attttccttt caatactgcc atgctaccag
    48181 cttcttcttt tttgtcctgc cttactaaaa tcaagagggc gttaagcgta aacaaagcta
    48241 caactagagc gctaatcaaa ccatctcttg tcaaaatact agataggact aggccaagca
    48301 agagaactcc tccttgcgcc agtaaatact tgccatgaag ctcatagatt gtcataccag
    48361 caagacgttt aatcataagc tcccgtctga actgctcaaa atagagaaga ttcatggaat
    48421 caaataacaa gatagccgta gacagggtca aaatcgtccc aataaggaga gagtaaaact
    48481 ctacccgttt gtcatttagt accttggcaa aaaatagctg gctttttacc aagtaagaaa
    48541 ccttgtgata cagaccttgc tcttttagag ctgtgatgct gtctccatat cgatctagtt
    48601 tcaaggcact attagccgta gttccccaca acatccctgc aacaggtctt gttccagtcg
    48661 cttgcggcgt tagaactaca ataatcggat cttttaggta ctggcgtgtt gtcttgtacc
    48721 catcattata gaggaaacgt tcctgtcctg tttctgtaaa agctagactt acctgtggga
    48781 ggtagtattt ctgactcgtc acttctacac tttcagatga aaagttttgc aggtaatctg
    48841 taaacaatct ttggtagtag gtagactgct ctcgcaagct ctcaggcaag attagcccaa
    48901 actctccctc agacaagttt tgcatcttgt ccataaactc tgaagaaacg gtaatctttt
    48961 cttctatcag atagcgtggt gagacataga taacattccc tgacggtgtg tagtcactga
    49021 gacgattgcc atctaggtcc acttctgctc cgtctgagaa attgtcaaca ttgctcataa
    49081 tataaaaaga gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac tcacgattat
    49141 ccttacgcgt tccttcttca tcggcaaatg cactagacca accaaaggat agacggtaac
    49201 ggtctgagga ctggctccat ttattgctag ctctctccat ttcctgcatt tcacggtagt
    49261 ggggtaggag agctgtcgca ctcgatccga ccaccaatac agctaagagt tgccccacca
    49321 tcatcaatgt catcatacgt ttgagaggga gtttcccttt caatagatcc accagactat
    49381 tttcctgtaa accaagtaga tagacgacac ttagtaaggt agaaatccct gccagggtca
    49441 atccataaag tagaagagca atgatgacca gttgcaccgt tgccataaac aaggcacctt
    49501 gataccagag aatccccaat cccaaaagac tggataccag cactgagcag ataaactgtc
    49561 tcacatcttc taacactggt ctgagagcaa ctccaaacaa gctctcacca gctattaagc
    49621 gaatccctgc ctgacgaagg gatttgatcc gataaatcag ggtcagactc ataaaggtca
    49681 gcagaaaaat agctaaactc agtagcacca taggggtggc cgccaatagt aagactatcg
    49741 aaaatggatc ttctccattc gaaacaaagc cttgatagcc aagttctttc aaggtggtct
    49801 gtaagctcac tccatccaaa cttcctgaca caatcaagta agaccctaat aaatcactcg
    49861 tttctgcact ctccttggag gaaatggtca gaccttctgg aagctctccc tcaccataag
    49921 tggcataggt aaagagtgtt tctccagctt catttggctc aacgattcga cgggcgatga
    49981 gactattatg ctctaccgcc aagtgactca aaacctctgt cacctcttcg cgactagcta
    50041 ctggttgatt tatcatcatt cctgtcacat caacagaagg atagttacca aataaaatct
    50101 cttctcgatg gtcaacggaa gttatcacca tataaagcga taccaatacc attgaaatca
    50161 aaataaataa acgtttcatc atttcctcca aaaagttact tggtcactta gtgagaaggt
    50221 gaccacagtc actgcgatgt taagaagctt attaagttct ttctactagc aacatcttca
    50281 aatacattat atccgttttt tcaagaacta tcaaggacat caatgtcctt aaaatctaac
    50341 tatttcatat tatttttagc ttatatctaa aaaaacagtt ctaaagacga actttagaac
    50401 tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta taaatatttt
    50461 ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaatccatc tccaattagc
    50521 ttcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc tctttcttct
    50581 cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata atcttgagtt
    50641 ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt tactcctacc
    50701 ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagaa gttttctaat
    50761 ttagttacct ttttctgttc caaaagaaag agcattagct tttcgatttg atttataaat
    50821 ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa caacatatca
    50881 tttgttgaat aaacttcttt ttctatgatc tgagacatat gttcctcaag tattaactct
    50941 gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac ttcacatgct
    51001 aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc caaacacgct
    51061 tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat ctggtatttc
    51121 aattctaaat aagcaggagg aagagctgaa aaatccggca taaggctata aactggaact
    51181 cctagccggc gagcaatata ggctaacttg ggcaaactcg gttgcgaagc tccactttca
    51241 attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaata tggacgacta
    51301 actccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag acgcctcctc
    51361 ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggcta catctttcct
    51421 actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac aactctaggc
    51481 gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg agatattctc
    51541 tacgcttacg gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg ctacgactaa
    51601 tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc gttttcaggt
    51661 cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac tttggaacag
    51721 cttcatctcg cttgagaaag gcatgccgaa ctgttggaat ggccgtcata atcatgcgcc
    51781 gaatccgctc cccattaaaa tctgggtctg taaagacaat gactccatga cgttggtgca
    51841 ggcgctgaat ccgctctata tcctgagcat tgatggcaga acctcgagtc tcataggtct
    51901 ccacatcgaa ataacgtttg agattgaccg tatcatcacg cccttcaacc acgataactt
    51961 gagaaatttt ctctttcatt acttgctgtc caatccaaaa atgcgttctg catttgcagt
    52021 cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa agtcgaccac
    52081 atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg ctaagtaagg
    52141 cgcatctgtt tccaccaaaa tcttgtccaa aggtaactct ttagctgctt cttggaggtc
    52201 agttgccttc ttaaaagtca ccactcctga gaaggaaatg gtcataccaa gatccacaaa
    52261 cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac gaggaccaac
    52321 gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat ggacaacaaa
    52381 aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct cctgcacctc
    52441 tttgggcgct gtcatccaat ggtaatctaa gccaatttca cctaaagcca caaccttggg
    52501 atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac cagcttctgt
    52561 aggatgccaa ccaatagtcg catagagctg atcatactca tctaccaact ccaaggcacg
    52621 ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca tctcagcagc
    52681 caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt gtgtatgtgt
    52741 atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa aaagaggagg
    52801 ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct gctatctttt
    52861 ctatccgata ttgcccatct cctattccac agttagagac agaagagatt ggctacattg
    52921 atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact tttctcaccc
    52981 atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa gtagagaata
    53041 gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa gcaaaaccac
    53101 ttgttgccac cactagacca agactagcaa gtgctgcact tgatgctgaa cctgtgttag
//
LOCUS       DAVBCR010000004        48619 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCR010000004 DAVBCR010000000
VERSION     DAVBCR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.004"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(47..1936)
                     /locus_tag="VQ508_000201"
     CDS             complement(47..1936)
                     /locus_tag="VQ508_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2489264.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2074..3639
                     /locus_tag="VQ508_000202"
     CDS             2074..3639
                     /locus_tag="VQ508_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2489265.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIALPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3639..4379
                     /locus_tag="VQ508_000203"
     CDS             3639..4379
                     /locus_tag="VQ508_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2489266.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4488..4703)
                     /locus_tag="VQ508_000204"
     CDS             complement(4488..4703)
                     /locus_tag="VQ508_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489267.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VQ508_000205"
     CDS             complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VQ508_000205"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2489268.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6874..7230)
                     /locus_tag="VQ508_000206"
     CDS             complement(6874..7230)
                     /locus_tag="VQ508_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2489269.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7468..7704)
                     /locus_tag="VQ508_000207"
     CDS             complement(7468..7704)
                     /locus_tag="VQ508_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489270.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VQ508_000208"
     CDS             complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VQ508_000208"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2489271.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VQ508_000209"
     CDS             complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VQ508_000209"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2489272.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9368..11068)
                     /locus_tag="VQ508_000210"
     CDS             complement(9368..11068)
                     /locus_tag="VQ508_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2489273.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDILPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11268..12935)
                     /locus_tag="VQ508_000211"
     CDS             complement(11268..12935)
                     /locus_tag="VQ508_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2489274.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12938..13303)
                     /locus_tag="VQ508_000212"
     CDS             complement(12938..13303)
                     /locus_tag="VQ508_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2489275.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13460..13648)
                     /locus_tag="VQ508_000213"
     CDS             complement(13460..13648)
                     /locus_tag="VQ508_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2489276.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13764..13940)
                     /locus_tag="VQ508_000214"
     CDS             complement(13764..13940)
                     /locus_tag="VQ508_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2489277.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14051..14200)
                     /locus_tag="VQ508_000215"
     CDS             complement(14051..14200)
                     /locus_tag="VQ508_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2489278.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(14512..16056)
                     /locus_tag="VQ508_000216"
     CDS             complement(14512..16056)
                     /locus_tag="VQ508_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2489279.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16269..16571
                     /gene="gatC"
                     /locus_tag="VQ508_000217"
     CDS             16269..16571
                     /gene="gatC"
                     /locus_tag="VQ508_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2489280.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16571..18037
                     /gene="gatA"
                     /locus_tag="VQ508_000218"
     CDS             16571..18037
                     /gene="gatA"
                     /locus_tag="VQ508_000218"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2489281.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18037..19479
                     /gene="gatB"
                     /locus_tag="VQ508_000219"
     CDS             18037..19479
                     /gene="gatB"
                     /locus_tag="VQ508_000219"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2489282.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19608..20168
                     /gene="efp"
                     /locus_tag="VQ508_000220"
     CDS             19608..20168
                     /gene="efp"
                     /locus_tag="VQ508_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2489283.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20190..20579
                     /locus_tag="VQ508_000221"
     CDS             20190..20579
                     /locus_tag="VQ508_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2489284.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20572..20994
                     /gene="nusB"
                     /locus_tag="VQ508_000222"
     CDS             20572..20994
                     /gene="nusB"
                     /locus_tag="VQ508_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2489285.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21043..21420)
                     /locus_tag="VQ508_000223"
     CDS             complement(21043..21420)
                     /locus_tag="VQ508_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2489286.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21761..21946)
                     /gene="briC"
                     /locus_tag="VQ508_000224"
     CDS             complement(21761..21946)
                     /gene="briC"
                     /locus_tag="VQ508_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2489287.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22105..22872)
                     /locus_tag="VQ508_000225"
     CDS             complement(22105..22872)
                     /locus_tag="VQ508_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2489288.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22869..23735)
                     /locus_tag="VQ508_000226"
     CDS             complement(22869..23735)
                     /locus_tag="VQ508_000226"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2489289.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23772..25139)
                     /gene="accC"
                     /locus_tag="VQ508_000227"
     CDS             complement(23772..25139)
                     /gene="accC"
                     /locus_tag="VQ508_000227"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2489290.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25151..25573)
                     /gene="fabZ"
                     /locus_tag="VQ508_000228"
     CDS             complement(25151..25573)
                     /gene="fabZ"
                     /locus_tag="VQ508_000228"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2489291.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(25570..26055)
                     /locus_tag="VQ508_000229"
     CDS             complement(25570..26055)
                     /locus_tag="VQ508_000229"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2489292.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26058..27293)
                     /gene="fabF"
                     /locus_tag="VQ508_000230"
     CDS             complement(26058..27293)
                     /gene="fabF"
                     /locus_tag="VQ508_000230"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2489293.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27315..28046)
                     /gene="fabG"
                     /locus_tag="VQ508_000231"
     CDS             complement(27315..28046)
                     /gene="fabG"
                     /locus_tag="VQ508_000231"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2489294.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28080..29000)
                     /gene="fabD"
                     /locus_tag="VQ508_000232"
     CDS             complement(28080..29000)
                     /gene="fabD"
                     /locus_tag="VQ508_000232"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2489295.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(28993..29967)
                     /gene="fabK"
                     /locus_tag="VQ508_000233"
     CDS             complement(28993..29967)
                     /gene="fabK"
                     /locus_tag="VQ508_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2489296.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(30086..30310)
                     /locus_tag="VQ508_000234"
     CDS             complement(30086..30310)
                     /locus_tag="VQ508_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2489297.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30370..31344)
                     /locus_tag="VQ508_000235"
     CDS             complement(30370..31344)
                     /locus_tag="VQ508_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2489298.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31344..31778)
                     /locus_tag="VQ508_000236"
     CDS             complement(31344..31778)
                     /locus_tag="VQ508_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2489299.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31872..32657)
                     /locus_tag="VQ508_000237"
     CDS             complement(31872..32657)
                     /locus_tag="VQ508_000237"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2489300.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            32982..34346
                     /locus_tag="VQ508_000238"
     CDS             32982..34346
                     /locus_tag="VQ508_000238"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2489301.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34349..34720
                     /locus_tag="VQ508_000239"
     CDS             34349..34720
                     /locus_tag="VQ508_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2489302.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34934..36208
                     /gene="serS"
                     /locus_tag="VQ508_000240"
     CDS             34934..36208
                     /gene="serS"
                     /locus_tag="VQ508_000240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2489303.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36233..37132)
                     /locus_tag="VQ508_000241"
     CDS             complement(36233..37132)
                     /locus_tag="VQ508_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2489304.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37242..37790)
                     /locus_tag="VQ508_000242"
     CDS             complement(37242..37790)
                     /locus_tag="VQ508_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2489305.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38008..39330)
                     /locus_tag="VQ508_000243"
     CDS             complement(38008..39330)
                     /locus_tag="VQ508_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2489306.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39421..39509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39673..42009)
                     /locus_tag="VQ508_000244"
     CDS             complement(39673..42009)
                     /locus_tag="VQ508_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2489307.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42113..42661)
                     /locus_tag="VQ508_000245"
     CDS             complement(42113..42661)
                     /locus_tag="VQ508_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2489308.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42658..42960)
                     /gene="zapA"
                     /locus_tag="VQ508_000246"
     CDS             complement(42658..42960)
                     /gene="zapA"
                     /locus_tag="VQ508_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2489309.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43047..43928
                     /locus_tag="VQ508_000247"
     CDS             43047..43928
                     /locus_tag="VQ508_000247"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2489310.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            43932..44546
                     /gene="lepB"
                     /locus_tag="VQ508_000248"
     CDS             43932..44546
                     /gene="lepB"
                     /locus_tag="VQ508_000248"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2489311.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            44674..47040
                     /locus_tag="VQ508_000249"
     CDS             44674..47040
                     /locus_tag="VQ508_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2489312.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47088..48371)
                     /locus_tag="VQ508_000250"
     CDS             complement(47088..48371)
                     /locus_tag="VQ508_000250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2489313.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14232 a        11150 c         9165 g        14072 t
ORIGIN      
        1 tctatcttcc attatatcat actttttcag tctatggtag ctctctttaa aaggctccga
       61 tactgccgcc acctccgcct ccagagaagc caccgccaga acttccactt ccagaagata
      121 cggagtaggt acttgctgta tttgcgacac tagcataatg gctcatttgc gcgcttgaat
      181 gataaaacat actgtgccag ccataagcta catagagatt gatatctgga ttttcaactt
      241 gaatctgatg aaccttcatc aaatgactaa ccttgtccgc atagccaaat aaggttgcat
      301 agaccaagag acgattccaa acaacaatac tttccaattc agccttatcc agtcgtgcaa
      361 tctcacgtaa catattttca aaactggtcc agagatagta gacctcagct cccgcttcat
      421 ttagaacacc atcacgatta tctagtcgaa gcttccaata atagaaaaca gacaaaacta
      481 accctagaaa accaagtatt ggcaaaggga ggtaaagata gccatgaacg tctaaactgt
      541 acaagaacaa accaaatccg ataaataggg gcaagatagt caagacaccc atacccactt
      601 gcaaagtctt ttccaaacca gttaaaggac gatagtaatc tgggagcccc cagaaggaaa
      661 ctcgttttct cactccttct tgcatctggt tcaatacctc ttcaaaagaa gatttgagtt
      721 gaagccctct tgcttgaatc cgtttttcat cagaaacttt ggctctacga taaagactat
      781 cagatacctt gtaatccgca aacaaattgg aaagagtggc ttcttttttg cctgaaaagg
      841 ctagatttag acagtctctc tcaaatcttg acaaaccatc ttctttcacc aatctcaagc
      901 caactgcatc tccttctgaa ataatagaga cattcccacg gtctatcaca tctagcaagg
      961 tagcttgaat aagttgatca aaggtgaatt ttccagctcc cttgaccaag ggactcactt
     1021 cctccaagga ggtcgagtag actgcttctg ataaaaccat aggctctaat tccattggtg
     1081 gttcatagag acgatgattt ttggcatatt tgactgaagg agtggtcttt cttctataaa
     1141 taaaatagaa gcagacactc aataacaagg agatagaaag tatcgaaggg aggacccaag
     1201 taacgagttg tttactctga tctttttctc taacaatcga gtcttctatc ttattaaact
     1261 cttctaaacg attccctttc aagccctgat ccctagcgct agcaaaatcg gtccgagacc
     1321 aataggcatg caactcaact ccacgcttag ccggaagatt gtctaaacgg atagtataat
     1381 caaggttact cttttcaatc gttccctctc taaaaagttt ccctgtatgg aaaaagagtt
     1441 tttcagcccc cttgtctccc cttacatgaa attcaaactt ttcaatagac tctgaactat
     1501 ctgtcagagg ttgccaattt aattcagcga tatcatcata aaggaaaagt aaatttttta
     1561 agttccagac gaggtcaact tcaactatgt cgccctcctg acctggatta tagactctca
     1621 cagtataacc atccgcttct tctgttactt cgctagtcac atctgctagt tctgcaccgt
     1681 ttttcgcagc ctgaaccttt ggatgagggt caatgtcaaa cccgctaggc atcttaccag
     1741 cacgtccaag tcccacgatt tggcccttaa agtcctcctc aaactggtaa actatcttct
     1801 gtctaaactc tgccgtatta tctgcatgaa tatacaaatc cccttgataa gagtttatct
     1861 tgaaatcaat ggcaaaaaca gagagtggaa gaaggcaaaa caagcctaac accagtaaga
     1921 aaaaagtttt tttcatcaac taagccccct ttttatcttt cttatcatta tagcatcttt
     1981 tcgtaagtaa aggcttactt atgttgaaaa attacgcatt tttcgataaa ataggagaca
     2041 gttatttttt aatagatcag aataggagaa atcatgagaa aaatatactt atctattttc
     2101 acaagtctct tgctgatgct aggacttgtc aatgttgctc aagccgatga atatttacgc
     2161 atcgggatgg aagcagcata tgctcccttt aactggaccc aggatgatga tagcaacgga
     2221 gctgtcaaaa tcgatgggac caatcagtat gccaacggat acgatgttca aatcgccaag
     2281 aaaatcgcta aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta
     2341 gtccctgccc ttacttctgg taagattgac atgattatcg caggtatgag tccaactgca
     2401 gaacgcaaac aagaaattgc cttttcgagc agttactaca ctagcgaacc agttttgctt
     2461 gtcaaaaaag attctgccta cgcaagtgct aaatctttgg atgactttaa tggtgcgaaa
     2521 atcacttctc aacaaggtgt ctacctctac gacttgattg cacaaatccc aggtgctaaa
     2581 aaagaaacag ccatgggaga cttcgctcaa atgcgccaag ctcttgaggc tggtgtcatt
     2641 gatgcttatg tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc
     2701 aagatgattc aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc
     2761 gggcttcgta aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca
     2821 aaagatgacc aagttgcctt gatggatcgt atgatcaagg aacaacctgc cgaagctaca
     2881 acaactgaag agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac
     2941 tggcaacaac tcttgcgtgg tgctggtatc actcttttaa tctctatcgt cggaaccatc
     3001 ataggtctca ttattggact tgccattggt gtcttccgta ctgctcctct ctctgaaaac
     3061 aaagccattt atggccttca aaaactagtc ggctgggttc tcaatgtcta cattgaaatt
     3121 ttccgtggta cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct
     3181 ttcgggatca accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt
     3241 gcctacatga ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa
     3301 gctgcgactg ctcttggtat gacccataac cagaccatgc gtaagattgc cctacctcag
     3361 gtagtccgta acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca
     3421 tctgtattga acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca
     3481 caaacctatc aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc
     3541 ttcaccgtaa cacgtatcct acgatttatc gagcgccgaa tggacatgga tacctatact
     3601 acaggtgcta accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa
     3661 ttaaacacct caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tcactcactg
     3721 tccacaaggg agaggtcatc tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc
     3781 tacgctccat taacctactt gaaacaccaa ctgatggaca aatcctttat catggacaaa
     3841 acgtcctcga aaaaggctat gacctcacgc aataccgtga aaagttgggg atggttttcc
     3901 aatcctttaa cctctttgaa aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa
     3961 ctgtcctaaa acgcgaacgc acagaagctg aaaagattgc caaagaaaac ctggaaaagg
     4021 tcggcatggg agaacgctac tggcaagcca aaccaaaaca actctcaggt ggtcaaaaac
     4081 aacgtgtggc catcgctcgt gccctctcca tgaatccgga cgctattctc tttgatgaac
     4141 caacatcagc tctcgatcca gaaatggttg gagaagtcct caaaatcatg caggacctgg
     4201 ctcaggaagg cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct
     4261 ctcaccgtgt tatctttatg gataagggcg tgatcgctga agaaggtaaa ccagaagacc
     4321 tcttcaccaa tcctaaagaa gaccgaacaa aagagttcct tcaacgctat ctcaaataaa
     4381 aagaaaaggc tgcatcaacc gtgcagtctt tttgctgtcc tcaaaatgaa aaggcagatt
     4441 ctctatctct actacttgaa ggtcaagaat ccgccgttat ccaaagatta atcttcaaat
     4501 ttttcataat ttttttcata gaaatcaatt aagcctaggg ctgtttcaaa tgaaatattt
     4561 tttacttttg cacgaccctg cgccagagca atgatagaca tttcacgcgc attcgtttct
     4621 ttagagatac ggtagcctgt gattttctta tcacgaaccc agccaacaac tgattctact
     4681 ttttcaaagt ttgacttagc catgtttttc tcctattttc ttctttataa tatttaattg
     4741 tatacgtttt tactactttt gtcaataaaa aaaacatata ttcaacgaaa ataattagtt
     4801 cagaattttc ttacttcctt tttaaatccg ataatatata ggttttgcta gcctctacac
     4861 cataaatatt tgctaataaa actactgtat tattaccctc ttaaggtgcg aaattattca
     4921 agttcttaaa aaatgcttct ctatttccgt aagatagctt atttcttcga tttaagaact
     4981 gaagatgata gttctaacct ttagaaatcc aagccttcac tagctcaaaa actcctatct
     5041 ttcttcctca ataaatcatc tgagagatag gtattcctta tatccttcaa attcatcact
     5101 cctttggata ttgaggtgct agcctagaag cagacacccg attatctaaa ccatccttaa
     5161 tagagatgac aagacagata agctcagtta attacttata tatcttttta atatggtata
     5221 tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa tgaaaatcaa agagcaaact
     5281 aggaagctag ccgcaagttg ctcaaaacac tgttttgagg ttgcagatag agctaacgtg
     5341 gtttgaagag attttcgaag agtattaaag acagtgtgac tatgcttgtc aatagatagg
     5401 aaatcaaaat gaaggatgga aatctgagcc aaacatcata agtgctataa tgaacagaag
     5461 taagaattgc acgataagtt ttctaaaacc ataggaatcg tcccctttct tcatcttcat
     5521 tatagcatat atacatcagt tgtacaaata atatgatatt tttttattag tcctcagaca
     5581 agcatattat aaagcgtttc attaccattt aagttaatat aagctggatc aaaaccttcc
     5641 attctacgaa tcaaacctgc ataatcatgc ttatctgcta aagcgatccc aatcaatact
     5701 gggcctgttc ccttgctagc tcgtttgata tactcaaaac gtgtgatatc atcatttggc
     5761 cccaggatat catttacaaa ctcacgcaaa gctcctggac gttgtgggaa attgaccaca
     5821 aaataatgtt tgataccatc ataaatcaag gcacgctctt ccatttctgg catacggttg
     5881 atatcattat ttcctccaga aatgatacaa caaatggttt tccccttaat atattcagct
     5941 aaaacctcta aagaggcgat actagctgct ccagcaggtt ctgcgactat cccttgctta
     6001 gagtaaaggt caatcaaggt ttcagaaatc aatccctcat cgacacctac caaagtttga
     6061 atatgttgac gagttgcttc ataggtcaac tgacctacct tttgcacagc aatcccatcc
     6121 gcaaatttat caatttcctt gagtttgact ggacctccag cctcaaaggc agctttcatg
     6181 gaacgcgctc cattcgcctc tactccgata acctcaatct ctggacttgt ttccttgata
     6241 taggtagaaa ccccggcaat gagaccgcca ccaccaacag gaaccaagac agcatcaaaa
     6301 tcaatcgatt cttttcgagc ttcttctaaa atctcataag caactgttcc ttgacctgct
     6361 tgaacatgag catcatcaaa aggatcaata aaggtacgat tttcagagac tgtaaattct
     6421 tgagctgctt tggctgaggc atcaaaggta tctccaacta gtttaatagt tacaaaatcc
     6481 ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg tagtaatagg cataaagata
     6541 gtagcaggaa ttttcatttc attacaagta taggctactc cctgcgcatg atttcccgca
     6601 gaagcgcaaa ctaccccacg ttcacgttct tccttgctga gctgggaaat ggcataatag
     6661 gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt cttttttcaa ataaatctta
     6721 gcaccatact tctccgataa ataatgatcg taatccagtg gagtattcac aaccacaccg
     6781 ttcaagacct tgtgagcctt gattatatct tttgaactta acatataaaa cctctttttc
     6841 tataaaaaac agtcactaat gactgttttt tagttattca tctccgaaaa tttcagtaac
     6901 actttcatgg tatttatgag ccatacgaat cgaaaactct ttaggaagtt cataacgatt
     6961 tgtggaaatc caatcatata accattctac caaactttct tcactatcat tacgatggaa
     7021 gataaaatag tttaaaattt ctttatattc tacatcattc aaatccaacg tgtctacaaa
     7081 ttgactacga ggaacaaaat tagaatctaa ttttcttagt tgatctgtca attctcccaa
     7141 ttgtaaatcc aactgaaaaa atagtttttg cagttgccta tcagtttcat cacatcctgt
     7201 cttgatagaa aacaaattgg taatattcat atgatttcaa cccctaaata agtacatcta
     7261 atatcccacg aatagctgaa gctaaagttt tcgcagtcca gtcatttaaa cccgattctt
     7321 tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc gtctcattta
     7381 ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca gctgatttag
     7441 gtcctaatga tttaccaact atatttgtta atatttttta tttctcaaaa tccatttcgc
     7501 tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa tttcaagacc
     7561 ttcttctcta gctatttgtg ccaatttgaa gaattgttca aaggtcatat gcaacctacc
     7621 atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat acgaccaatc
     7681 tgctttattt tggtctgcta gcactattac cgacggtgta attacattat acaactagaa
     7741 ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct ccagcgctat
     7801 atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca acgaatggca
     7861 ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct tgttcacggt
     7921 aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt gcaaatttac
     7981 cattttggat gtctgccaag acagccttca tattttcttt aacttgttca gtgattacac
     8041 gtggacctga tacatagtca ccgtattcag cagtgtttga aatagattga cgcattttct
     8101 tgaatccacc ttcgtagatc aagtcaacga tcaatttcat ttcgtgaaga acttcaaagt
     8161 aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct tcgataaggg
     8221 cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca gtttcttctt
     8281 tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac cagtccatag
     8341 caatgttttt agcatttcct gttgcatctt ggtatactgc ataaagagct ggaacaccaa
     8401 atccttcttc gtaagtacga cgtaccaagt gtcctggtcc tttaggagca cacatgaaga
     8461 catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca tgggcaaatc
     8521 caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat tcttgttgaa
     8581 tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct gctactgtgt
     8641 aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt acaccgataa
     8701 taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgcccttgt gaaccataac
     8761 cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt tcatattcca
     8821 tttgaactgt catagctttt ctctcttttc tattatttat tgccttttag gctggtttaa
     8881 caaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc gagcaatatt
     8941 gcgaataccg tatgggcgaa tgactctcaa tagggcttcg ctcttttctg catttcccgt
     9001 catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga aaggttgaat
     9061 aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca aaatcacctc
     9121 gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa tctgacgatt
     9181 gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa taatagtgat
     9241 acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat taacctgacg
     9301 acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta gttttgctgt
     9361 taacattcta cgcatggaac tgcaccccca acatctcatg attactctta ccagccggta
     9421 ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata ggaacatcct
     9481 cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta tagtttttaa
     9541 taccataagc ctgcgccatc aattggaaat caggaaggat atcaaagacc gactctgatg
     9601 ttctgccttc atagaaggat tcctgccact ggcgaaccat tccgagtgaa tgattgttca
     9661 gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc tggttagtca
     9721 tttggaaacc accatcccca acaaacaaga ctacttcctt atctgggtta gcaattttag
     9781 caccgattgc tgctggaatt ccaaagccca ttgttcccaa accacctgaa gtcactaact
     9841 gacgttcatt ttggtaggga taatactgag ctgtccacat ttggtgttga ccaacgtctg
     9901 ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact gcttgcggtt
     9961 gaaccacacg ctctttcttg tcataagaac gaacacgatt cttgtcttta gtgactttct
    10021 caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt tgcaaggcct
    10081 tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc tcagctgggt
    10141 caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc cccgtcaaac
    10201 ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg gcaatatttg
    10261 ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc gttgcaatcg
    10321 ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca aattcattta
    10381 gttccgtagc agcctcagca taactaattc caccaccagc taacaagact ggctttttag
    10441 ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca agagtcggct
    10501 gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa gcagatatgt
    10561 cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata tggacagctt
    10621 ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac ttagtgattg
    10681 gcatggtaat tcccacgatg tctgcctcct gaaaggcatc cttcccaatc cctgctcgcg
    10741 ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catggcatcc gcaatccctg
    10801 taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac tttccagttg
    10861 atttggcata accttcagct tcatgcaaac aaccttgctc atggcgccct agaatgtggc
    10921 gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca ccaggataac
    10981 caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc gaccccgtct
    11041 taggagattc taaactgatt ttctccatta ttcccctttc tttcctctta aaaataactt
    11101 gttactatca taccattttt tcaaaatttt tcaagataaa agaagaaatt ttctgaattt
    11161 tctattttaa cgtttattta tgaatgttat ttttgttttt attaaaaaga taccgatttc
    11221 atttactaaa aagaaaaaag aactgatttc tcagtccttc attaatctta ttccacacta
    11281 aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac gtcttcgaat
    11341 tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc ttcaccgaca
    11401 tagaaggtta cgatttcact atcttcatcc aacatatgtt tcaaggtttc agtcaatgtt
    11461 tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag attatcgttt
    11521 tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta
    11581 acgacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga cttgcttgga
    11641 tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac cactactgct
    11701 ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt gttgtttggc
    11761 aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc ttctgttgaa
    11821 gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca gaagatatct
    11881 gctagacctt taccagccac cacagcaatc aaagcatact ctttttcttc agccgacttg
    11941 ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact
    12001 tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc tggatcttct
    12061 gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga atccccaagc
    12121 tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt tggaccttgc
    12181 ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt tgctacgtga
    12241 ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc aggagtcgct
    12301 acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc ttcgtagatg
    12361 aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac tggaagcatg
    12421 tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat gacttcaaca
    12481 gtgtcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg agaaactgtc
    12541 aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac acctgattgg
    12601 aaggccagag ccaagtcttg acctgttaac tcgtctttat ccttgatagc ttgggaaaat
    12661 ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat caaaagccct
    12721 ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc aacttcttta
    12781 gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg aactggaaag
    12841 acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc agcctgcacc
    12901 atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca actttgatat
    12961 tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc aagctaaagg
    13021 caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag cttaacacgg
    13081 tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca cctttagaat
    13141 aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta ctagccatac
    13201 cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc acttcatctg
    13261 ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt acctcaacta
    13321 gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga tgtatcagcg
    13381 gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa agaccctaag
    13441 gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa gcacgagctg
    13501 aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga aggtttggtt
    13561 ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac
    13621 gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc taatatagcg
    13681 gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt tgcaagggaa
    13741 ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc tgctctccac
    13801 atgccatcgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa atcatctcta
    13861 actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat cgattttctt
    13921 acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt tggaataaaa
    13981 tacctaaaaa agccgcatat gaaagtccct caaaggggag acgataacct ttcagaatat
    14041 tactgtcaat ctaaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
    14101 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
    14161 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
    14221 agacaatcgc ttttttctct tttttctctt ctttcacttt ttatctcatt tctttttatt
    14281 cccatcatat gtttctacgt aaccattatt ttcgcctatt cgttcgtaaa accataccag
    14341 tggagatttt agatgaagtc ccattacggt ttacaatttt tacattacga cacggagttt
    14401 tacaaatcga tttcatttgc caaacgtagt tagtgaggca gttagctagt tcgccaaata
    14461 gcgactagcg tccaacaatt tggaacttta gttccaattg ttggtactga gtcacatctt
    14521 ctcctctaac tctacgtctg gatacttgtc agcaaaccag cggagggcaa agtcattttc
    14581 aaagagaaag actggttggt caaaacggtc tttggctaag atattgcgac ttgacgacat
    14641 ccgttcatcc aagtcctcag gcttgatcca acgaacggtc tttttaccca ttgggttcat
    14701 aactacttct gcattgtact cgccttccat gcggtgttta aagacttcaa actggagttg
    14761 acctacagcg cctagcatgt actcacctgt ttggtaattc ttataaagct gaacggctcc
    14821 ttcttgcacc aattgctcaa tccccttgtg gaaggatttt tgcttcataa cattcttagc
    14881 agaaactttc atgaaaattt caggagtaaa ggttggcagt ggttcaaatt caaacttgtt
    14941 tttaccaact gtcaaggtat ccccaacttg ataagtaccg gtatcgtaaa ccccgataat
    15001 atcacctgcc acggcattga tcacattctc acgactttcc gccataaact gggtaacatt
    15061 agatagtttg gcccccttac cagtacgagg gagattgaca ctcatgccac gctcaaattc
    15121 gcctgatacg atacggacaa aggcaatacg gtcacggtga cgaggatcca tgttggcttg
    15181 gattttaaag acaaagcctg agaaatcctt gtcataagga tccacaattt caccgtctgt
    15241 tttcttgtga ccatgtggtt ctggagcaaa cttgaggaag atttcaagga aggtctgcac
    15301 accaaagttt gtcagggctg aaccgaaaaa gacaggcgtc aattctccag ccagaatagc
    15361 ttcctctgaa aactcattcc cagcttcatt taaaagctca atgtcatcct tgacttgctc
    15421 gtagaaagga ttgctaccaa agagtttgtc cccgtcttct agactggcaa aacgctcatc
    15481 ccctttgtaa agctctaaac gttggttata gaggtcatac aagccctcaa aggctttccc
    15541 catcccgata ggccagttca tagggtagct agcaatgccc aagatttctt ccaattcttg
    15601 caagagatcc aaaggctcac gaccgtcacg gtccagcttg ttcataaagg taaaaactgg
    15661 aatgccacga tgtttcacaa cctcaaacaa tttcttggtt tgagcctcga tccccttggc
    15721 agagtccacg accatgaccg cagcatccac cgccatcaag gtacgatagg tatcttctga
    15781 gaagtcctcg tgccctggcg tgtctaagat attcacgcgc ttgccgtcgt agtcaaattg
    15841 cataacagat gaagtaacag aaatcccacg ttgcttctcg atatccatcc agtcagattt
    15901 agcaaaagtc cctgttttct tcccttttac cgtaccagcc tcacgaatct cacccccaaa
    15961 gtagagtaac tgctcagtga tggttgtttt ccccgcgtcc gggtgggaga taatggcaaa
    16021 ggtacgacgt ttcttaattt cttcttgaat attcataagt tctctttctt tgattctcta
    16081 tttttcttgt ttcaatagct gagaatgatt tttacattgg attttaccat tcctttcaac
    16141 attccattat atcggatttt agcatttttt tcaatttcta tttcttttca cttccccctc
    16201 ccttatttat aggaaaatat ggtaaaatag aacagactaa aaatcatcat ttcacgaaag
    16261 gatgcaagat gaaaattacg caagaagagg taacacacgt tgccaatctt tcaaaattaa
    16321 gattctctga agaagaaact gctgcctttg cgaccacctt gtctaagatt gttgacatgg
    16381 ttgaattgct gggcgaagtt gacacaactg gtgtcgcacc tactacgact atggctgacc
    16441 gcaagactgt actccgccct gatgtggccg aagaaggaac agaccgtgat cgcttgttta
    16501 aaaacgtacc tgaacaagac aactactata tcaaggtgcc agctatccta gacgatggag
    16561 gagatgccta atgactttta acaataaaac tattgaagag ttgcacaatc tccttgtctc
    16621 taaggaaatt tctgcaacag aattgaccca agcaacactt gaaaatatca agtctcgtga
    16681 ggaagccctc aattcatttg tcaccatcgc tgaggagcaa gctcttgttc aagctaaagc
    16741 cattgatgaa gctggaattg atgctaacaa tgtcctttca ggaattccac ttgctgttaa
    16801 ggataacatc tctacagacg gtattctcac aactgctgcc tcaaaaatgc tctacaacta
    16861 tgagccaatc tttgatgcga cagctgttgc caatgcaaaa accaaaggca tgattgtcgt
    16921 tggaaagacc aacatggacg aatttgctat gggtggttcc ggtgaaactt cacactacgg
    16981 agcaactaaa aacgcttggg accacagcaa ggttcctggt gggtcatcaa gtggttctgc
    17041 cgcagctgta gcctcaggac aagttcgctt gtcacttggt tctgatactg gtggttccat
    17101 ccgccaacct gctgccttca acggaatcgt tggtctcaaa ccaacctacg gaacagtttc
    17161 acgtttcggt ctcattgcct ttggtagctc attagaccag attggacctt ttgctcctac
    17221 tgttaaggaa aatgccctct tgctcaacgc tattgccagc gaagatacta aagactctac
    17281 ttctgctcct gtccgcatcg ccgactttac ttcaaaaatc ggccaagaca ttaagggcat
    17341 gaaaattgct ttgcctaagg aatacctagg cgaaggaatt gatccagagg ttaaggaaac
    17401 aatcttaaac gcggccaaac actttgaaaa attgggtgct atcgtcgaag aagtcagcct
    17461 tcctcactct aaatacggtg ttgccgttta ttacatcatc gcttcatcag aagcttcatc
    17521 aaacttgcaa cgttttgacg gtatccgtta cggctaccgc gcagaagatg cgactaatct
    17581 tgatgaaatc tatgtaaaca gccgtagcca aggttttggt gaagaagtga agcgccgtat
    17641 catgctgggt actttcagtc tttcatcagg ttactacgat gcctactaca agaaggctgg
    17701 acaggtccgt acacttatca ttcaagattt cgaaaaagtc ttcgcggatt acgatttgat
    17761 tttgggtcca actgctccaa gtgttgccta tgacttggat tctctcaacc atgacccagt
    17821 tgccatgtac ttagccgacc tcttgaccat acctgtaaac ttggcaggac tgcctggaat
    17881 ttcgattcct gctggattct ctcaaggtct acctgtcgga ctccaattga ttggtcccaa
    17941 gtactctgag gaaaccattt accaagctgc tgctgctttt gaagcaacaa cagactacca
    18001 caaacaacaa cccgtgattt ttggaggtga caactaatga actttgaaac agtcatcgga
    18061 cttgaagtcc acgtagagct caacaccaat tcaaaaatct tctcacctac ttctgcccac
    18121 tttggaaatg accaaaatgc caacactaac gtgattgact ggtctttccc aggagttcta
    18181 ccagttctca ataaaggggt tgttgatgcc ggtatcaagg ctgctcttgc cctcaacatg
    18241 gacctccaca aaaaaatgca ctttgaccgc aagaactact tctaccctga taatcccaaa
    18301 gcctaccaaa tttctcagtt tgatgagcca atcggttata acggctggat tgaagtcgag
    18361 ctagaagacg gtacgaccaa gaaaattggt atcgaacgcg cccacttaga ggaagacgct
    18421 ggtaaaaaca cccacggtac agacggctac tcttacgttg accttaaccg ccaaggggta
    18481 cctttgattg agattgtatc tgaagccgat atgcgttcgc cagaggaggc ctatgcttat
    18541 ctgacagccc tcaaggaagt tatccagtac gctggcattt ctgacgttaa gatggaagaa
    18601 ggttctatgc gtgtggatgc caacatctcc cttcgtcctt atggtcaaga gaaattcggt
    18661 accaagactg aattgaagaa cctcaactcc ttctcaaacg ttcgtaaagg tcttgaatac
    18721 gaagtccaac gccaggctga aattcttcgc tcaggtggtc aaatccgcca agaaacacgc
    18781 cgttacgatg aagcgaataa agcaaccatc ctcatgcgtg tcaaggaagg ggctgctgac
    18841 taccgctact tcccagaacc agacctaccc ctctttgaaa tttctgacga gtggattgag
    18901 gaaatgcgga ctgagttgcc agagtttcca aaagaacgtc gtgcgcgtta tgtatctgac
    18961 cttggtttat cagactacga tgctagtcag ttgactgcta ataaagtcac ttctgacttc
    19021 tttgaaaaag ctgttgccct aggtggtgat gccaaacaag tctctaactg gctccaaggg
    19081 gaagtcgctc agttcttgaa tgctgaaggt aaaacactgg aacaaatcga attgacacca
    19141 gaaaacttgg ttgaaatgat tgccatcatc gaagacggta ctatttcatc taagattgcc
    19201 aagaaagtct ttgtccatct agctaaaaat ggcggtggcg cgcgtgaata cgtggaaaaa
    19261 gcaggtatgg ttcaaatttc agatccagct atcttgatcc caatcatcca ccaagtcttt
    19321 gccgataacg aagctgctgt tgccgacttc aagtcaggca aacgtaacgc cgacaaagcc
    19381 tttacaggat tccttatgaa ggcaaccaaa ggccaagcca acccacaagt tgcccttaaa
    19441 ctacttgcac aggaattggc gaagttgaaa gaaaactaga cagaacaaaa ccagccctaa
    19501 ggttggtttt ttcttctcta ccaactccca ataactattt tggctttatt tccagagtat
    19561 tttatggtaa aatgaagagt aataatattt attaaagagg taaaaacatg attgaagcaa
    19621 gtaaattaaa agctggtatg acctttgaaa cagctgacgg caaattgatt cgcgttttgg
    19681 aagctagtca ccacaagcca ggtaaaggaa acacgatcat gcgtatgaaa ttgcgtgatg
    19741 tccgtactgg ttctacattt gacacaagct accgtccaga ggaaaaattt gaacaagcta
    19801 ttatcgagac tgtcccagct caatacttgt acaaaatgga tgacacagca tacttcatga
    19861 atacagaaac ttacgaccag tacgaaatcc ctgtagtcaa tgttgaaaac gaattgcttt
    19921 acatccttga aaactctgat gtgaaaatcc aattctacgg aactgaagtg atcggtgtca
    19981 ccgttcctac tactgttgag ttgacagttg ctgaaactca accatctatc aaaggtgcta
    20041 ctgttacagg ttctggtaaa ccagcaacga tggaaactgg acttgtcgta aacgttccag
    20101 acttcatcga agcaggacaa aaactcgtta tcaacactgc agaaggaact tacgtttctc
    20161 gtgcctaatc tctagaaaga ggtcattcta tgggaattga agaacaactt ggcgaaatcg
    20221 ttatcgcccc acgtgtactt gaaaaaatca ttgctatcgc tactgcaaag gtagagggtg
    20281 ttcactcttt ttcaaacaga tcagtgtctg ataccctttc aaaactttca ctcggccgtg
    20341 gcgtttatct taaaaacgtg gacgaagaac tcacagcaga tatctatctc taccttgagt
    20401 acggagtaaa agttcctaag gtagcggttg ctatccagaa agctgtcaaa gatgccgtcc
    20461 gtaatatggc tgatgtagaa ctcgctgcta tcaatattca cgttgcaggt atcgtcccag
    20521 ataaaacacc aaaaccagaa ttgaaagatc tatttgacga ggacttcctc aatgactagt
    20581 ccactattag aatctagacg ccaactccgt aaatgcgctt ttcaagctct catgagcctt
    20641 gagttcggta cgaatgtcga aactgcttgt cgtttcgcct atactcatga tcgtgaagat
    20701 acggatgtac aacttccagc ctttttgata gacctcgttt ctggtgttca agctaaaaag
    20761 gaagaactag ataagcaaat cactcagcat ttaaaagtag gttggaccat tgaacgctta
    20821 acgctcgtgg agagaaacct ccttcgcttg ggagtttttg aaatcacttc atttgacact
    20881 cctcagctgg ttgctgttaa tgaagctatc gagcttgcaa aggacttctc cgatcaaaaa
    20941 tctgcccgtt ttatcaatgg actgctcagc cagtttgtaa cagaagaaca ataaaaagaa
    21001 aaagtgtttg atagatatca aacacttttt ctttgcctcc cactatctaa aaaaatgata
    21061 atagatataa ttgtaaacaa aaatccagat aggttttgca tgattgagaa agttaaaaaa
    21121 actatggcag agaatcgtta atctcagatt gtcggtagaa cgataaacaa gggcaaaaaa
    21181 gaaaccaatc agactataat ataataaact aattggatct ctgtgagata gtatcaaatg
    21241 gctaatccca aagataaggc ctgataagat gacatctaaa tagtactttg acttaggaaa
    21301 gaaggtattc ataaaatacc ctctatcaag agtctcctca aaaacaggac cgatgattac
    21361 aagcaggaca aaagataaga tagtcgataa aaaggttggt tgtccatttg aaagaaccac
    21421 ggtaaaatac tcatcatgaa tattcctatg gttaatcaaa tgagcatagc gtgcctaaaa
    21481 attaccgaga atctgataaa ccacataagt tgcaaataag gagaagacaa atgaccagtc
    21541 ccagctcttt ttctttttta acctccaaat taatagaaaa ggaaacttcc cactaatccc
    21601 actgttaaaa taagagaata gacatcagct cctaacccta aaatgatcgt cacatacaat
    21661 ccaattgttt gtggtaaata ggtagatagt aaaataataa gcaaaaatat tccaaattgt
    21721 cttagtttct ttgtgtttct catcgtactt ttttgaaaga ttaccctgtt cggaagccgt
    21781 acttccaagc atctatataa gaattaagtg ccccttgcct catataggga gcaaattctc
    21841 tataatataa ccatctacta tatccatctt cccaaacagc aagaccacct gaagtttgct
    21901 ccaagtcctc agttgaaaga actgtaaatg tttctgtacc tgtcattgca agtaccttct
    21961 taaaatagat tgttgtaggc tcacatttat agtatatttc tttttttgtc tattttatag
    22021 tctatctcct caactggcaa tttttcgacc tgaattgcat ttttccataa aaaatgagac
    22081 ctttctagtc tcatttagtc attcttagta ttttctaaat cgttgatagc gttcttccag
    22141 caactcttct agcggttttt gtgaaagtct agccagctcc gtttggagtt cttttttgac
    22201 actcttaatt agttctttac tagaaagtcc tacttcagaa atcaccttat ccaccacgtc
    22261 catttctaac agttcatgcg aagtgatttt catcagttct gctgcttcca tggcgcgagt
    22321 accgtccttc cataaaatgg aagcaaagcc ttctggactg agaatggcat agatagaatt
    22381 ttccagcatc cagacacggt ccgcgacagc tagagccaga gccccgcctg aaccaccttc
    22441 accgataata atggcgataa taggaacttt caggtcactc atttccatga gattgcgagc
    22501 gatagcttcc ccttgaccac gttcttccgc tccgacacca ggataagcac ctgctgtatt
    22561 gataaaggtc acaactggac ggccaaattt ctcagcctgt ttcatcaacc gcagtgcctt
    22621 tcggtagcct tctggatgtg gttggccaaa attccgtttg aggttgtctt gcaaactctt
    22681 gcctttttgg ataccaacca ctgttacagc ttggtctcca agccaaccaa taccaccaac
    22741 aactgcacca tcatcacgaa aagaacggtc accatgtaat tggataaatt catcaaaaat
    22801 gcctgtcgca aagtccaagg ttgtcaagcg actctgctca cgcgcttctc tgactatttt
    22861 tgcaatattc atctaggact ccctccatgc aatctgacta ggctagcaat cgtatctggt
    22921 aagtctcttc ttttgacaat agcatccaca aagccatgtt ctaataggaa ttctgccttt
    22981 tggaaatcct caggcaagct ttcacgaacc gtattttcaa tcacacgacg cccagcaaaa
    23041 ccaaccaagc tctgtggttc agccagaatg atatcgcctt ccatagcgaa agaagctgtc
    23101 acaccaccag tcgttggatc tgtcaaaatg gtcaggtaaa agagaccagc atttgaatgg
    23161 cgtttaaccg ctgcagagat cttagccatc tgcatgagac tcatgattcc ttcctgcata
    23221 cgggctccac cagaggctgt gaataggaca actggtaatt tttcgacagt cgcatactca
    23281 aacaaacgag tgattttttc acctacaacc gtacccatag aagccatgat aaaattagaa
    23341 tccataatcc caagagccac agtctgacct ttaataagag cagttcctgt cacaacggct
    23401 tcatgcagac ctgttttttc acgcatagat gccagtttct tttggtaacc agggaaatgc
    23461 aagggatcct tgctttcaat ccctgtaaac aattctttga aggttcccat atcaatcgtc
    23521 aaagccaagc gttcttgggc agaaatacga aaggtatagc tacagtgcgg acagatacgt
    23581 tcacttccca gatccttctg atagatggta tgcttacagc ctggacactg ggaaaataat
    23641 tcatctggaa cctctggctt agcttgaggt ttttccctaa ccgaacgatt gggattgatt
    23701 cgaatatact tatctttttt actaaataga gccattgatt ccccttttcg gtttaaactc
    23761 ttaaagtcat tttattcttt ttcttgatat ttaggtaaga aggtttccat caagaaggaa
    23821 gtatcataat ccccagcaat gacattgcga tctgaaatga gatcaagctg gaaatctgca
    23881 ttggtctgca ctccttcaat ctctaattca tagagggcac gttgcatttt catcaaggcg
    23941 tcaaaacgat tttcgccgtg tactatgatt ttggcaatca tactatcata ataaggcgga
    24001 atggtataac ctggataaac tgctgaatcc acgcgcaagc caactccacc acttggcaga
    24061 tagagattag taatcttacc tggacttgga gcaaagttaa aggctgggtt ttctgcattg
    24121 atacgacact cgatggcatg accgcgtagg acaatatctt cttgcttaac agacaaaggc
    24181 tgacctgccg caatgcaaat ctgttcctta acgatatcaa cacctgaaac aaactctgtt
    24241 actggatgtt ctacctgaac acgagtattc atctccatga aatagaaatt gctacttgct
    24301 tcatcaagaa gaaattcaat ggttcctgca ttctcatagc caacaaactc tgccgctcga
    24361 acagcagcag cacctatttc atgacgcagc gtttttccga ttgcaatcga gggactttct
    24421 tccaaaacct tttggttatt cctttgaaga gaacaatccc gttcacccaa gtgaatcaca
    24481 tgtccatgct catcacctag gatttgaacc tcaatgtgcc gagctggata gataacccgt
    24541 tctatgtaca tggcaccatt gccataattg gccttggcct cactagaggc agtttcaaag
    24601 gcagaaacga ggtcatctgg tttttcaacc ttacgaatcc ctttaccacc tccacctgct
    24661 gaagccttga gcataacagg atagccaatt ttttcagcaa caatcaaagc ttcttcagag
    24721 ttatgcactt ctccatctga acctggtata acaggcacac ctgctttaat catctgagca
    24781 cgcgcattga tcttatcccc catcatatcc ataacatgac cagatggacc gataaacttg
    24841 atacctactt cttcacacat ggtcgcaaat ttggaatttt cactgagaaa tccaaaacca
    24901 gggtgaatag cttctgcctc agtcaagact gcagctgata gaactgcatt aatattgaga
    24961 taagactctg ttgccttgcc aggaccaata caaactgctt catctgccaa cagcgtatga
    25021 agagcttcct tatcagcagt tgaataaacc gctaccgtcg caatccccaa ttcacgtgcc
    25081 gcacggataa tacgaaccgc aatttcacca cgattggcaa ttaaaatttt tcgaaacatg
    25141 gagaacctcc ttagttccca attgcaaaag taagggtacc actggctgca agcttgccat
    25201 ccacttcagc ctttgcttca accacagcta tggtgccacg acgttttaca aaagtcgctg
    25261 tcataaccaa ttggtcgcct ggtacaactt gcttcttgaa cttaacctta tccataccag
    25321 cgtaaaagac cagttttcct ttattttcag gttttgataa ctccaacaca ccagcagttt
    25381 gcgccaaggc ttccataatc agaacacctg gcataactgg gtattgagga aagtggccgt
    25441 taaagaaagg ctcgttgatg gtcacatttt tgatagcaac aatggtatcc tcgctcacct
    25501 ccaagacacg gtccactaga agcataggat aacggtgggg aagggcttct ttgattcctt
    25561 gaatatcgat catttgatac gtaccaatcc tttaccaaac tcaaccattt cttcgttaga
    25621 gacgagaatt tccgttacca caccatcctt aggagctggg atttcattca tgactttcat
    25681 ggcttcgata attaccaatg tttgaccttt tttgacacta tcaccaactg taacgaaggc
    25741 aggtttatct ggtccagcag ccaagtaaac cactccaaca agtggactct ctacaagatt
    25801 tccctcacta gccacacttg cttcagctgg agctggaact tcttctgcta cagtctctgc
    25861 tggagcagat gtaggagcta ctggactcgg tgttgctaga acgggtgctg gagcgacttg
    25921 agttgcaact tcaggcacag gtctcgcttc attcttgcta aactgcaact catccgtccc
    25981 atttttataa gaaaattctc tcaaacttga ctggtcaaat tgagtcatca agtctttaat
    26041 atcgtttaaa ttcatactta tctattctcc caacgtttga aagcaagaac tgcattgtgg
    26101 cctccaaaac caaaagtatt tgaaatagcg tatggaattt ctttctccaa gccttgtcca
    26161 taaacgacat tagcttcgat ataatctgat acttcacttg tcccagctgt cattggtaca
    26221 aagttatgac gcatagcttc gatggtgacg atagcttcta ctgcacccgc agcccccagc
    26281 aaatgtcctg taaaagactt ggttgatgat acaggtactt ccttaccaag aacagctacg
    26341 atagcaccac tttctccttt ttcattggca ggagttgacg ttccgtgagc attgacatag
    26401 gctacttgct ctggagaaat ctcagcttct tccaaggcta gtttgatggc cttgatagct
    26461 ccctgacctt ctggatgtgg agaagtcatg tggtaggcat cacaagtatt tccgtaacca
    26521 accacttcag ccaggatagt agctccacgt ttttcagcgt gttcaagact ttctagaacc
    26581 aacatccctg aaccttcacc cataacaaac ccattgcgat ccttatcaaa tgggatcgaa
    26641 gcacgagttg gatcctctgt agtagagaga gctgttaagg cttggaaacc agcgatggca
    26701 aaaggtgtga tagaagcttc tgttcctccc accaacatca catcttggaa accaaactta
    26761 atggagcgga aggcatcccc aatcgcatca tttgatgaag agcaggcagt attgatagat
    26821 ttacaaacac cgtttgcacc aaaacgcatg gctacattcc cagaagccat atttggtaaa
    26881 gcttttggaa gagtcattgg tttgacacgt ttgggtcctt tttcatgaag gcgaagtacc
    26941 tgatcttcaa tttccttgat tccaccaata ccagatgcaa cgataacacc aaaacgatcc
    27001 ctattaagag cctctacatc aagattggca tgatttacag cctcttgggc tgcatacaag
    27061 gcatataaag aatagttatc aaaacggttg gtatcttttt ttacaaagta tttatcgaac
    27121 ggaaaatctt ggatttctgc cgcattatgc acatcaaagt cactatgatc aaattttgta
    27181 atgccaccaa tgccgatttt cccagttgct aaactattcc aaaattcttc tggtgtattt
    27241 ccgattggag atgttactcc ataacctgtt accactactc gatttagttt cattcttttc
    27301 acctctagct ttcgctacat acttaagcca ccatcaatgg caatcacttg accagttaga
    27361 taatcttggc ctgctaaaaa tactgtcaaa tctgcaacct gctctgcctg cccaaattct
    27421 ttcatcggaa tctgagctag tgtagcttcc ttaatcttat ctgataagat agctgtcata
    27481 tcagactcaa tcattcctgg agcaatcaca ttgactcgta tattccgact agcgacctcg
    27541 cgtgccacag acttggtaaa gccaatcaag ccagccttag aagcagcata gttagcttga
    27601 ccaatattcc ccatcaaacc aacaacacta gacatattaa tgatagcacc ttctctggct
    27661 ttcatcatcg gtttcaagac tgattgtgtc atattaaagg caccagtcag attgaccttg
    27721 agcacttttt caaaatctgc ttctgtcatc ttgagcataa gagtatcttg ggtaatccct
    27781 gcattgttga ccaaaacatc tactgaaccc agttctgcaa tagcttgatc aatcatacgc
    27841 ttagcgtctg caaaatctga tacatctcct gaaatgggaa ccaccttgat accatagttt
    27901 gaaaactcag cgagcaattc ttctgagatt gccccacgac tgtttaagac aatgttggct
    27961 cctgcttgag caaacttgtg ggcgatggca agaccaattc cacgactcga acctgtaata
    28021 aagatatttt tatgttctag tttcattttt ttcctttcaa aacttctact tattttagtc
    28081 tatttttcta aaagtgctac taaactcgct tgatcttcca catgagctaa gtgagcagtt
    28141 tgatcaattt ttttaacaaa acctgacaag actttccccg gtccaatctc gataaagttg
    28201 cttatgcctg cttcttgcat gaccccaata ctttcataga aacgaacggg ttccttgacc
    28261 tgacgcgtca agagctgagc aatgtcctct ttttgcatca cagcagcttc tgtattgccg
    28321 actaggggac aagtaaaatc tgaaaaactt acctgagcta gagtttcagc tagtttctgg
    28381 ctagcaggtt caaggagagc ggtgtgaaag ggacctgaca ccttaagagg aatcaagcgt
    28441 ttggcacctg cttcttgcaa aagttcaacc gctcgatcaa ctgcaaccac ttctccagca
    28501 atgacgattt gtgcaggtgt gttatagttg gctggagtaa ccactccaag ttcagaagct
    28561 ttttgacagg cttcttcaat gacctctact ggcgtattga gaactgctac catcttgcca
    28621 gagtcagcag gagccgcttc ttccatatag gctccacgct tagctaccaa ggcaaccgca
    28681 tcttcaaaat ccaaggcgcc gcttgccacc aaggcagagt attctccaag agacaaacca
    28741 gcaaccatat caggctgata gcccttttct tgcaataaac ggtagatagc aaccgaagtc
    28801 gctagaatgg ctggttgcgt atagcgggtc tgattgagtt tgtcttcttc cgtatcgatg
    28861 agataacgta aatcataacc gagcacctga ctcgctcgat caatcgtttc tttaacaatc
    28921 ggatactgat catagaaatc ccgtcccatc cctagatact gggcaccttg accagcaaat
    28981 aaaaaggctg ttttagtcat ttcttacaac tcctgcccag cgagaggctt cttcttgaat
    29041 tttcttagcg gctccgtaat acaaatcttt taggatttct tcagctgttt cttctttaga
    29101 aacaagccct gcgatttgac ctgccatgac agagccaccc tccacatcac cgtgaacaac
    29161 tgctttggct agggcacctg ctcccatttg ttcaaagatt tctaaatcag gatcttcctg
    29221 cttaaaggca tctttttcag ccagttcaaa atctctagtc aactgatttt taatagcacg
    29281 aacagcatga ccaaagtgct gagctgaaat cgtagtatca atatcccttg cttttaaaat
    29341 tttctctttg tagtttggat gggcattcga ctcttttgca actacaaacc gtgtccccac
    29401 ctgtacagcc tctgcaccta gcataaagcc agccgcagca ccttcaccat ccgcaattcc
    29461 tcctgcagca ataacaggaa tagatatagc tgtggctacc tgtcgcacca aggtcatggt
    29521 tgttaattta ccgatatgcc ccccagcttc cattccttct gcaataacag cgtctgcacc
    29581 gattttttcc atgcgtttag ctaaagcgac actaggaaca acaggaataa cgattatccc
    29641 agcttcatgg aaacgttcca tatacttgct tggatttcct gctcctgttg tgacaacttt
    29701 aacaccttct tcaataacga gatccacgat atcttccaca aagggagata agagcatgat
    29761 gttgacccca aagggtttat cagtcaatga tttgatttta tcaatattgg ccttgacaac
    29821 ttctttcggg gcatttcccc caccgataat tcctaatcct ccagccttgg aaacagcccc
    29881 tgccaaatca ccatcagcaa cccaggccat ccctccttgg aaaataggat agtcaatctt
    29941 caataattct gtaatacgcg ttttcatagt gcctccaacc ttccttgctt acgtaatagt
    30001 tcgatttcac cataatttga cagtcaaact attacctaaa caagagggag tgggtttctc
    30061 cctactcctt ctactaatat tctgcttatt ttgcttgctc ttcaacgtaa gcaaccaagt
    30121 caccaactgt tttcaagtca ttttctgctt cgatttggat atcaaaagca tcttcgattt
    30181 ctgagattac ttggaacaag tccaatgaat ctgcgtccaa atcatcaaaa gttgattcaa
    30241 gtgttacttc tgatgcgtct tttccaagtt cttcaacgat aatttcttgt actttttcaa
    30301 atactgccat gataggactc ctttaaaata aatagttttt ttataacaat gtgttcacca
    30361 catgattacc taaattgtaa gaatgagcgt gccccaggtc aagcctccac cgaagcctga
    30421 tagaagaaca gtttggctac catctaaagg gatgagacct tgttctacac actctgaaag
    30481 taaaatcggg atactggctg cactggtatt gccatattcc atcatattag ctggaagttt
    30541 ggctcggtca acaccaattt ttctagccat cttatccaaa atacggttat tggcttgatg
    30601 aagtagcaga taatccaagt ctgtcacctc tataggagat tcatcaatag tctgcttgat
    30661 agacttggct acatctcgaa tggcaaaatc aaagactgtg cgtccatcca tcttcaaaaa
    30721 tgaatctgca ctttcttgat ctgaaaatgg agaatgcaaa cctgaatgcc cataagttaa
    30781 acactcgctg cgacttccat cgctattgag actctcagct aagaaatgct cttgctcgct
    30841 agcttctaac aagacaccac cagcaccatc tccaaacaac acagctgttg atcgatccga
    30901 ccaatcgact gccttagaga gggtttcact accaatcacc aagccttttt gaaagcgacc
    30961 agaagcgata aacttttcag cagttgaaag agcaaataca aatccactgc aagccgcggt
    31021 taagtcaaaa gcaaaggctt tattagcacc aatattagct tgaacacgag cagctgtaga
    31081 gggcatcatc gaatctggag taatggtagc taggatgata aaatccagtt cttctcctgt
    31141 tattccagct tttgccatca gtttcttagc aacctctgta gccaaatcac tggtagattc
    31201 tgttcttgaa atatgccttt gtcgtattcc cgttcgactt gaaatccact catcattggt
    31261 atccataatc tgagccaagt cgtgatttgt aaccacttgc tctggcacat aatgagcaac
    31321 ctgacttatt tttgcaaaag ccattatttc aaatcctcca aaaattggta aagattagtc
    31381 aaacctttac ccatgacagc aatttcttcc tcgctcatgc catcaataat tttttctacc
    31441 atggccttgt ggaagcgttt atgcagtcta tgaatcaagc gacccttctt tgtcaaatgc
    31501 agatgcacca cacgacgatc ctgttctgac cgaactcgct caatgtagcc cttacgctct
    31561 aaattgttca aacttgtcgt aacagttcca agagttacca tcaactcttt tgacacttga
    31621 cttggagtca catctggagc cttaccgatg acatcaatcg tatgcatttc tttgatggag
    31681 atatccttaa aacgactacc tctcaagttc acttcctcaa ttacaaggac attgttaaat
    31741 atagatgtta aatattcatt gattcgttgg tagtccattt ttcatatccc tccttcttca
    31801 aaaaactttt acaatcaaaa catttgacag aaagaattat atcaaaattc aaagaatgat
    31861 gcaagtattt tttattttcc tgtaaattta ggtcttcttc tctccgaatg agctcgcact
    31921 ccttctttga aatcctctgt ttgagctaag gatttctgta ggttcagttc taaagtagcg
    31981 taaccctgcc agtctttaaa ttggctctcc caaaccaact tcttaatggc agcataggaa
    32041 ttacttgaag cacgtcttaa ttttttaaga agctgttctc tcgtcttttc aagtttttca
    32101 gcttcagaaa cgcggtaaac caacccccat tctagggctt tttctgccgt taaagcctct
    32161 cctgtcatag ctaattgagc agcacgcgtc acaccgatac tgcggctcaa gagatgaatt
    32221 ccccctgcat ctggagccaa accaacacca acaaaggctt ggataaactt agccttatcc
    32281 gtcgccaaac agaaatctgc cgcaacagcc atattcgctg cggcacctgc aacagcaccg
    32341 tcaacttcca ttaaaacagg tttggctatt tgcttgattt tataagaaat cgtattgacc
    32401 aactctgcga tttttgtcaa tgatggaata tcatcctcat ccactgcccg cttcatctct
    32461 accaaatctc ccccaactga gaagaccttt ccattggcat tgattaagat aaaatgcaca
    32521 gctggatttt cttctgccaa agtcagagct tctaaaatct cctcacacat gggaatatga
    32581 aaaccatttg cgacctcagg acggttcaag gtaaggattg ccaagtcctc ttcaagctga
    32641 taaataatgt gttccatcag ggctcctttt tattttataa gcagtttata ctcttcgaaa
    32701 atctctttaa accgcgtcag cttccatctg caacctcaaa acagtatttt gagctgactt
    32761 cgtctagctt cttagtttgc tctttgattt tcattgagtg ttaaattatt tcactgtcaa
    32821 agtatatctt ttttaggagc agaaagcaag aaaaaatcga ctgaaagtgt ctcagccgat
    32881 aagaaaggct cgctttaggc tagaaagcat cttattttct tcattgaaaa aagcctttct
    32941 ttctgctata atcgtataaa atacttactt taggagttct tatgaaagtt gttaaatttg
    33001 gaggtagttc tcttgcctct gctagtcaat tagaaaaagt tttaaacatc gtcaaaagcg
    33061 attcagagcg tcgttttgta gtcgtttctg cgcctggtaa acgcaatgct gaagatacta
    33121 aggttacgga tgccctgatt aaatactacc gcgactatgt tgcgggtaac gatattagca
    33181 agagccaaag ctggattatc gaccgctatg ctgctatggt tagtgaattg ggactaaaac
    33241 cagctgtgct agaaaaaatt tctaaaagca ttcacgcctt ggccactctt cctattgaag
    33301 aaaatgaatt tctctacgat actttcctag cagccggtga aaataacaat gccaaattga
    33361 ttgctgccta ctttaaccaa aatggtatcg atgcacgcta tatgcaccct agagaagctg
    33421 ggattgtggt cacaagtgaa cctggtcacg ctcgcatcat tccatcaagt tatgacaaga
    33481 ttgaagaatt gacaaacacc aatgaagtcc ttgtcattcc tggtttcttt ggtgtcacta
    33541 aggaaaatca aatctgtact ttctcacgtg gaggttctga tattacaggt tctatcattg
    33601 ctgctggtgt caaagctgac ctctatgaaa actttacgga cgttgatggt atctttgcag
    33661 cccaccctgg tattatccac caaccacact cgattcctga gttgacctac cgtgaaatgc
    33721 gcgagttggc ctatgcaggc ttctcagtcc ttcatgacga ggctcttctt cctgcctacc
    33781 gtggaaaaat tcctctggtt atcaagaata ccaacaaccc tgaccatcca ggtactcgta
    33841 tcgttctaaa acacagtaat gatgaatttc cagttgtggg aattgctggt gactcaggct
    33901 ttgtcagcat taacatgtcg aaatacctca tgaaccgtga ggttggattt ggccgcaagg
    33961 ttctgcaaat cctggaagaa cttaacatcg gttgggaaca tatgccaaca ggtatcgacg
    34021 atctttctat cattctccgt tctcgccaac taactcccat caaggaagaa gaaatcctgc
    34081 gtcagttggt tcaaaaggct aaagtagatc atgcagaaat cgaacacgac ctttctatca
    34141 ttatgattgt tggtgaaaaa atgaagagcc atatcggagt gactgctact gcgacacgcg
    34201 ctctatctga aaacaaaatc aacatccaga tgatgtctca aggttctagt gaagtttcta
    34261 ttatgtttgt tgtcaataaa gaccaagaga aagcagcaat taaagccctc tacaatgcct
    34321 tttttggaga aagtaaggaa gactaagcat ggcccaatca cttaacaaaa cagtactcct
    34381 cagtacgaca ggcacttcct acctctctat cgctgggaaa gttgggaaat tccttgtcgg
    34441 agatcaggct ttggaatttt acccagatgt caatgtcgaa caatttatcc agattccttg
    34501 gagccatatc aatcagattg gagccaatgt cactggacgc aaaatcagcc gccacttcga
    34561 agtctttaca gacaaaggaa aattcctctt tgcttcaaaa gactcaggtg ccattctcaa
    34621 aattgcacgc gaaaaactag gcaatgacaa ggtcgtcaaa cttccgaccc tgattcagac
    34681 aattagccaa aaatttaaaa atctatttgc aaaaaagtag aaactttagt ataatcatag
    34741 caacggataa gtaggttatc ttcttctttc agaaagtctg cgggtactgc gagcagatag
    34801 gggggatagg tgaaattcta ccgttagtaa caattacata cacaatcaag tgcacacctg
    34861 tgaagttgga tggaaccgtg gccctgccac tccaacgttt tgtcaggcgt gcttttttca
    34921 taaaggagtt cttatgttag atatcaaacg tattcgtaca gattttgaag ctgtcgcaga
    34981 aaaattagct acacgtggtg tagatgctgc tgtcttgaat gaaatgaaag aaatcgatgc
    35041 taaacgtcgt aacatcttgg tcaaggttga aactctcaaa gcagaacgta acacagtttc
    35101 tgctgagatt gcccaagcta agcgcaacaa ggaaaataca gatgacaaga ttgctgccat
    35161 gcaaaatcta tctgctgagg ttaaagcctt ggatgctgaa ttggcagaaa tcgatgctaa
    35221 attgacagaa tttacaacga ctcttccaaa tatcccagct gacagcgttc ctgttgggac
    35281 tgacgaagac gacaatgtgg aagttcgccg ttggggtact ccacgcgagt ttgacttcga
    35341 acctaaagct cactgggatc tcggtgaaga ccttggtatc cttgactggg aacgcggtgg
    35401 taaggtaaca ggcgctcgct tcctcttcta taaaggcctc ggtgctcgtt tggaacgtgc
    35461 tatctacaac tttatgttgg atgaacatgg aaaagaaggc tatactgaag tcatcacacc
    35521 ttacatagtc aaccatgatt ctatgtttgg tactggtcag tatccaaaat ttaaggaaga
    35581 tacttttgaa ctcagcgata ccaactttgt cttgattcca actgctgaag ttcctctgac
    35641 aaactactac cgtgatgaaa tcttagacgg caaagatctt ccaatctact tcactgccat
    35701 gagtccgtca ttccgttctg aggctggttc tgccggtcgt gatacgcgtg gcttgatccg
    35761 tttgcaccaa ttccacaagg ttgaaatggt caaatttgcc aaaccagaag aatcttacga
    35821 agaattggaa aaaatgacag ccaacgctga aaacattctt caaaaactca accttccata
    35881 ccgtgtcgtt gctctctcta ctggagatat gggcttctca gctgcgaaga cttacgactt
    35941 ggaagtgtgg attccagcac aaaacaatta ccgtgaaatc tcaagctgtt caaacacaga
    36001 agatttccaa gcccgtcgtg cccaaatccg ttaccgtgat gaagcagatg gcaaggtgaa
    36061 actccttcat accttgaacg gctctggact tgcagttgga cgtacagtgg ctgcaattct
    36121 tgaaaattac caaaatgaag atggttctgt gaccatccca gaagcacttc gtccatacat
    36181 gggtggagct gaagtcatca aaccataaaa aataaggttt agctatttct agctagacct
    36241 tttttcgtaa ccaaatcaga taagcaccta gtacaaagaa taaaatagtt aggcatataa
    36301 tggtttcagc caataccagg taatccagaa atggaagttt caaaattccc tgagccatct
    36361 tgagcgaggt cgctgtgata atggttggga aggtgagggc tgagaaggct ggttgaaaac
    36421 cttgttttaa aatgttgggc agacgagtta aaacaaagaa aaagaaggat tgagaagcca
    36481 aaatcatgac aatcaagacc caagtcggca ggctggttcc tcctactcga actagagaag
    36541 ccaagagtag agagaaagga gcacagtaga ttccttcttg tccaagcaag gctagtggga
    36601 gtggatgttt ctttaaatcg ctataaataa ggggatagag atagaaggtc aagagaaaac
    36661 caaaactcaa ggtcgcatag gcaatttcga taatacctac cagaggatag gtcaaggcag
    36721 ccactgctat ccccacatag agaaccgtcc aacttggagt ggcatgaacc ctccgccctg
    36781 aacaagcaaa cttgatggta aaaccagcaa tcaaggtcaa atccaagaga aatgaaaacc
    36841 accaaatccc ttgtgctacc aaaggaagat aagagaatac gcgaaagaca taggtcgata
    36901 aaatcatccc agccatagga aaggttgcca ttcctgacaa aagagggggc ttggtcaatt
    36961 cttgcttggt ttctttccaa ttaaagagat gcagaattag aaagtaaatc cataaaacca
    37021 aaccagtcag actaaaaaga tgggatagaa ccggcaacgt atctaaaata agatttccag
    37081 ctcctgccaa acctagcaaa caacctgaaa atactaaggg gagttttttc atcctaacct
    37141 ccaataatca tgttagtttc agtataacat aaaagcgctt aaatgaggat ttaaaaaaac
    37201 gaagtccgct tatttcagac ttcattttac tcagatatga attaggcata aggttgcaat
    37261 tctggattaa ttggtgtatt agctaagttg ttggcatagt tacagaggat tgctaggctg
    37321 acaccaaaaa ccacatccaa ggcattttgt tgagtgtagc cagcttctaa aaactcagac
    37381 aaggcttcat ctcctacacg acccttggta ttgataactg ccaaggtaaa cttagctagg
    37441 gtatccaatt taggatctgt ttcaattgga gtacgattgc gaagagcttg aatcaagtca
    37501 tcattcatct ggatttgttt gatggaaaag gctgtgtgac ctgcgacaca gaaggcacaa
    37561 ccattggtca cggctgccgt gatttgcacc acttcacgct caacgggtgt caggctgttg
    37621 cgacggtgga tagatgagac aatttggtag gcttctaaaa cagtcggggc attggccaag
    37681 agaccgatta ggttgggaat atagccattg ttgtcttttt ctactgtttc aagaatttct
    37741 ttcacttctg ctggtgctga ctctactgta tggatagtaa atgttgtcat aagatacctc
    37801 ttttcttatt attgacacta atattattgg aaaatcttat aaaatcctga ttcctaagtt
    37861 tatctaagat aaagctttat tctctcataa gattttcgtt gttatattag tttatcacac
    37921 ttccaatcac ttgcataata tatattatat atcaggctga taaaaattat ttataggcaa
    37981 aaaaatcaca cgagctgtgt gattccatta tttgtcaaaa tactttttag tttcagcaat
    38041 aacgactggc gacaagacca agagggcaat caagtttggc agagccatca aggcgttaac
    38101 gatatctgcg ataatccaga ccatatccaa ctcgataaat cctcctaaca agaccatgag
    38161 cacaaaaaac acacggtaga gccagataaa gcgagcccca aagaggaact caaaacagcg
    38221 ttctccgtaa tagttccaac ctagaatcgt tgtaaaggca aaaagtacaa ggaagatggt
    38281 caagagggca ggcccaaagt gtgaaaagac tgttgagaaa gctgactgag tcaaggcaac
    38341 cccattcaag tcaccactcc aaactccagt taccaagatg gtcaaaccag ttagagtaca
    38401 aatgatgagg gtatcaataa aggttcctgt catggaaatc aaaccttgct ctactggttc
    38461 atttgtcttg gcagctgcag ctgcaatagg agcagaaccc agaccagatt cgtttgagaa
    38521 cacaccacgc gccacaccat tttgaatagc catccgaacg ctagcaccag caaatccacc
    38581 taccgcagca aggggactaa aagctgaggt aaagactaaa gcgattgtgc cagggatttt
    38641 tccgatatta aagaaaataa ctgtaagagt tcctaagata taaatgaggg ccataaaagg
    38701 aacaacagta gttgaaacct tagaaataga cttgagtcca ccaaagactg caatcgctac
    38761 aaagacagac aagacgagag ctgtgatggc tggcgaaatc gtcgttgtat tttggataga
    38821 ttctgtaatc gagttgactt gggtgaaggt tccgattccc aagagagcaa ccaatactcc
    38881 tgctactgca aacaaaacag caagtggtcg ccacttttct cccatcccta gaaggatata
    38941 gtgcatggga cctcccgcta ctgcaccatg gtcgtccttg gtgcggtatt tgatggccaa
    39001 gagtccttcc gcatacttgg tagccattcc aaagaaagcc gccatccaca tccaaaatag
    39061 agctcctggt ccaccaacct tgatagccgt cgcaactcct atgatatttc ctgttccaac
    39121 agttgatgcc aaggctgtac acagagctgc aaaactggat acatcaccat gtcccttatc
    39181 ctggataaaa ataagctgaa aggccttggg cagacgcaaa acctgcaaga gtcctagccg
    39241 catggttagg taaatccctg ttccgaccaa taaaatcaag aggggcggtc cccaagcaaa
    39301 agcatcgatt gatttaagca attctaacat ttccttctcc tatcgtttca accccaaaag
    39361 aaagagcaca tgcaagatac atgtactctg gaatgcttag ataaatgcta aaaagcggtc
    39421 tatcctagct ctgtcctttt acctgagagt ttgagcagtt gcctgccttg ccccttcggt
    39481 gcctttacgg tctctccaga gttccgtcca tttacagtca tggaaaatca aacgattccc
    39541 cacttctatt aaacttcatt cggtgttggt atttaattga ttctaatttt acaaaaaatg
    39601 ttggcttttg tcaatgtgtt tattagtaaa aattagttca acagttttta ctttataaag
    39661 tccagaatac tgctatcctt taaaagtgac aatagtcgca ccactgcctc cagcattttg
    39721 tggggcatag ccgaaactct tgacatgttt gtttctttgc aagtatttgg taactccttc
    39781 acggatgact cctgttccga taccatggat gatatcaact tgagccatat tgttaagcaa
    39841 ggcttggtcg atgaaggtat ctagctcatt catggcttct tcatagcgct tgcctcgaag
    39901 atccagtcta gcttgaggtc ctcgcccaga agttcgtttc acaacattga cctgtttctt
    39961 cttgactggt ttttcttgct gggcttgaac aagatcaaac tctttctctt ccaaggtcat
    40021 cttaatcaag ccaacttggg cttcccagcg accgtccttg agttgactgg tcaaggtacc
    40081 acgctgacca taactgagaa ccacgatatc atctcccacc tttggagctc gttttttctt
    40141 ggccttttga aggaccttat ttttagacaa gtccactttt tcaggagcca attttttcaa
    40201 cttggccttg gcttcaatga tttcgtgggg cttgagttgg gatttactgt ggagattttt
    40261 gagaatctgg tcactttcac ttagggccat atccacaatc tcagcagcct gttcacgcgc
    40321 cttgttaagc tcggtttcct tttcacgatt aagctcgttg tagagttttt ttagcgcacg
    40381 gttcatcttg agattttctt gctccacctc acggatattg tccaaacgtt tgcggctttc
    40441 cagcgtctgc tcttctaatt gctcaatgat acgattgacg tcattgtcct gatcgatctg
    40501 ctgactggca tctcctacga taacttcaga taggcctaga cgtttggcaa tttcaaaggc
    40561 attacttcgg ccaggaacac cctgcataaa gcgataggtc gggcgaagag ttgcagtatc
    40621 aaactccata ctggcatttt gcacaaaggc tgtctcaata ccgtaggcct tgagttctgg
    40681 atagtgggtc gtcgccatgg tcttgatttg acgcaggcga aggtcctcca gaatagccat
    40741 ggcaagggct gctccctctt ggggatcagt accagccccc aactcatcca aaagtaagag
    40801 tgaatgttgg ttgaccttgc caagaatatc cacgatattg gtcatatggc tagagaaggt
    40861 agacaagctc tgctcaatag actgctcatc tccaatatca gcaaagattt cttcaaaaat
    40921 accaacacga cttcccttgt ctgctaaaat cggcaatcct gactgggcca tgacctgtgt
    40981 caagcccaga gttttgagca tgatggtctt cccacctgta ttgggacctg taatgacaat
    41041 agctgttaaa tcttgaccaa aatggacatc atttgcgacg gcatttttga ccaaaggatg
    41101 gcagacatgg agcagttgaa tctcttgatt ttctgacagc tgaggcacga ctgcttgtct
    41161 ttcttggata aatcgaacct tggcacgaat caagtccaga tgaccgataa tccaagcgtc
    41221 attagcaatc tcagccgcat gagggcggac acgctcagaa atttcttgga gaatgcgaag
    41281 catttcatag cgctcatctg ctcgcagact agcaatttct tcgctcagtt tgactacctc
    41341 acgtggttcg atatagacgg tgtttccact agcagaaata tcatgaacga cacctgcaat
    41401 cttattgcgg taggtgtttt tgactggtaa aacctgacgg ccatttctgc tagcaacaat
    41461 tccttccgtc aacatctgcg ctttttgctt gagcaagtct tgtaaaacat cgcgtacctg
    41521 actctcgcta tcatgtattt ttcgacggat tcgcgccaat tcttcactgg caaaattttc
    41581 aatgaaaccc gcatcattaa aggcctgaag atttccttgt aattgcggaa aatcatgtaa
    41641 tttctcaaac caaagggcta attcttccaa gctgacattt tccagattgg cataaaaact
    41701 ttgaagttct cggctggcaa gaagcacgcg tttcaagagt aggaactcct cgatattgag
    41761 atccgctccc atctccaacc tcttgcagac tcctgcaatt tccttagttg agagaatagt
    41821 aaaatgcggt tgctcgacga aaagagcctg catttctttc atctcagcaa aagcctgttt
    41881 gattttatct gctttggcag tcggagccag ctgtctcaat tgctccaagc cctgctcggt
    41941 caacaaatga ggctcaaaca aggccttgac cttatcgaac tctaatgttt ctaatatttt
    42001 cttattcatc tttatttcct tgtctttgaa tgtctaggtg tggtagcttt ttacattctg
    42061 atagattcga gtcaaatctg taaacaaagg gctaggaaaa ctcctgccct ttttatccga
    42121 ttaaatttgt cacccagatt tgtttgagcc aactggttgt tatcggtatg ctctggatga
    42181 tgtgttttgc gacgatactc ttttcaagag gattttgtat aactgccatg gggatggtcg
    42241 ccaagattgt caaggccatt tgcaagacaa ataaggtcac caacatggac aagatacctg
    42301 ctgaaacttg gaacaactta ccacccagtt ttttgctagg aatcaagtgt aagagaagac
    42361 caagcaaacg accaatgcta tagaaaatcc caaatacaag caagtagccg atacctgcat
    42421 aaaagacctt atccagctga aagagttgat ccgatgggaa aaagaaagtc ccctgacctt
    42481 cctgcgaatt tgcataaggg agcaataaat ggaattgctc tccaagcccc ttataaaact
    42541 ggccagccat aaaagccgat gccatggctg aaatcaggta ataaacctgt aagagcaggc
    42601 ctctccgata gccgatataa aatccccaaa ccaagaccaa tagaagaagg aatgaaatca
    42661 taaggaatcc tcaatcttgc tctgttcttg cttacaagtc acaagcttgt gacggagttc
    42721 ttctagctct tgctccttat cgtcaaattc aatctcacgg ctgagctgag ttgataaaca
    42781 gttgactgcc aacaaaagag cgattgtttc atcatctgcg ctaggcattt gttctttaat
    42841 tgcttggtat ttttctgtcg caaccttagc gatttcctcc ataaaaaggt tatcatgttc
    42901 gcttgtcaag gttaacgatt ttttcccgaa tgtaaatttg aatcgattta gatttgccat
    42961 aaaattcacc tcacgatatt ataccaaaat tcgctaattt tgtcagtttt tacaaatttt
    43021 actgcttttg tggtacaata gaaactatgg caagtatcac actcacacca agcgaaaagg
    43081 atattcaggc ttttcttgaa cactatcaaa ccagtctggc tcccagtaag aatccctata
    43141 tccgctactt tttgaaacta cctcaagcaa cggtttctat ctatacttct ggaaaaatct
    43201 tgcttcaggg tgaaggggct gagaaatacg ctagtttctt tggctatcaa cttgtagagg
    43261 aaaacagggg gcaaaatctt cctttaattg ggacagatga ggtgggaaat ggttcctact
    43321 ttggtgggct tgcagttgtg gctgcctttg tcacatctga ccagcacgac tttttacgaa
    43381 aactcggtgt gggggattct aagactctga ccgaccaaaa gatccgtcag attgctccta
    43441 ttctcaagga aaaaatccag caccaggcac tccttctctc acccagcaag tacaacgagg
    43501 tcatcggaga ccgatacaat gctgtttcgg ttaaggttgc cctccataat caggctatct
    43561 atctcctcct tcaaaaaggt gttcagcctg agaaaattgt gattgatgcc tttaccagtg
    43621 ctaaaaatta tgacaagtac ttggcacaag aggccaatcg tttcagcaat cctatcagct
    43681 tagaagaaaa ggctgagggc aaatacttgt ctgtcgcagt ttcttctgtc attgcgcgtg
    43741 atctctttct ggaaaatctt gaaaatttgg gacgagaact gggttatcag ctcccaagtg
    43801 gagctggaac ggcttctgac aaggtggcta gccagatttt gcaagcctat ggtatgcagg
    43861 gactcagctt ctgcgccaaa ttgcacttta aaaacactga aaaagcgaaa aaacgcttag
    43921 aaaggtaagt tatgaattca tttaaaaatt tcttaaaaga gtggggattg ttcctcctaa
    43981 ttctgtcatt actagcttta agtcgtatct ttttttggag caatgttcgc gtagaaggac
    44041 attccatgga tccgacccta gcggatggcg aaattctctt cgttgtaaaa caccttccta
    44101 ttgaccgttt tgatatcgtg gtggcccatg aggaagatgg gaataaggac atcgtcaagc
    44161 gcgtgattgg aatgcctggc gacaccattc gttacgaaaa tgataaactc tacatcaatg
    44221 acaaagaaac ggacgagcct tatctagcag actatatcaa acgcttcaag gatgacaaac
    44281 tccaaagcac ttactcaggc aagggctttg aaggaaataa aggaactttc tttagaagta
    44341 tcgctcaaaa agctcaagcc ttcacagttg atgtcaacta caacaccaac tttagcttta
    44401 ctgttccaga aggagaatac cttctcctcg gagatgaccg cttggtttcg agcgacagcc
    44461 gccacgtagg taccttcaaa gcaaaagata tcacagggga agctaaattc cgcttatggc
    44521 caatcacccg tatcggaaca ttttaagaaa cctaagaggc cgagaatcac caatctcagc
    44581 ctcttcttct atcgtgagaa aatgattggt aactatctaa acttaccaga acagaaacac
    44641 ctcaactctc acctattcat gcaaaggaat tttatggaag tttatttttc aggaaccatt
    44701 gaacggatta tttttgaaaa tcccagcaat ttttatcgca tcctcctcct agaaatcgac
    44761 gatacggacg cagaggattt tgatgatttt gaaatcattg tcacaggaac catggctgat
    44821 gtaattgagg gcgaagacta tactttttgg gggcaaattg tccagcactc caagtatgga
    44881 gaacaactgc aaatcagtcg ttatgatcgc gcaaaaccaa ctagtaaggg cttggtcaag
    44941 tacttttcaa gtagccattt caaggggatt ggtctcaaga cagctcagaa aatcgtggat
    45001 acctatggcg aaaataccat tgacgaaatt ttgcaacacc cagaaaagtt agaaggcatc
    45061 gcaggactct ctgccaaaaa tcgcgaggct ttcgtctcca ctctccgtct caactacgga
    45121 acggagatgg ttttggccaa actagccaac tacggcattc ccaacaaact agcctttcag
    45181 attcaagact tttacaagga agaaaccctt gatgtggttg aaaattatcc ctaccagctg
    45241 gttgaggata tcaagggttt gggctttacc attgctgacc aactagctga ggaactaggc
    45301 atcgaaagtc aggctcctga acgcttccgc gctggtctag ttcacagtct ttttcaggcc
    45361 tgtatggaaa caggggacac ctatgttgaa gcacgggatt tgctggaaca aacccttact
    45421 ctccttgagt cttcccgtcc cgtggaacta gaccccagcc aagttgccca agagctctcc
    45481 tacctgatcg aagaagacaa agttcagcag attgatacca agatctttga caacagcctc
    45541 tttttcgctg aggaaggcat ccgcagtcac ttggttcgta tccttgaaaa aggaaaacag
    45601 aagagtcatg atttaaaaac cattcaaaaa catatcacta ctatcgagaa agagcttgga
    45661 attgagtatg ataacattca aaaacaggct atctgtgatg ctatccagaa taaggtcttt
    45721 atcctgacag gtggacctgg tactggtaag acaactgtta tcaatgggat catcgctgtt
    45781 tacgccctgt tagagggact tgacctcagg aagaaaagca acctgcccat tcttcttgct
    45841 gctccaactg gtcgagctgc tcgtcgcatg aatgaattga caggtttgcc tagcgcgacc
    45901 atacaccgcc acttgggaat gacaggtgac gatgatacca gtcatctgga agattaccta
    45961 gatgctgact ttatcatcgt ggatgaattc tctatggtgg atacttggct ggccaatcaa
    46021 ctcttctcca gtatctcttc taacagtaag atcctcatcg taggtgacag tgatcagcta
    46081 ccttctgtca gtcctggaca ggttctagcg gatctgcttc atattccttt gattcctcag
    46141 actcgcttgg aaaaaattta tcgacaaagc gaagaatcaa ccatcgtcac cctagctagt
    46201 cagattcgac agggcatctt gccagctgat ttcacccaaa aaaaagctga ccgttcctac
    46261 tttgaaattg ctagtggcca tattcctgcc accattgaaa aaatcttagg tgccgccctc
    46321 agaaatggta ttcctgcccg tgatatccaa gttctggctc ctatgtaccg agggacggca
    46381 gggattgatg ctatcaatca gctcatgcaa gacctgctca atcccccaca aaaagatcaa
    46441 ctcagttttg aagctcctca gtgccactat cgtaagggag acaaggtcat tcatttggtt
    46501 aacgatgctg aaatcaatgt ctttaatggg gatttaggag ctatcacaga cctgattcct
    46561 ggtaaataca ccgaatcgaa acaagacgag attgtcattg attttgatgg taatgaagtc
    46621 tcttaccccc gtaacgaatg gtacaagatt cgcttggcct atgccatgag tattcataag
    46681 tctcagggaa gtgagttccc agttgtcatc ctaccgatta ctagtgctag ccgacgtatg
    46741 ctggagcgaa atctcatcta cacagccatt acacgtgcca aaagcaaact catcttacta
    46801 ggcgaattac aagccttcga ctatgctacc caacacatcg gaactgcccg aaaaacctat
    46861 ctgattgaac gcttcagtga tctactggag aatgttgaag aaaagcaaca agctgtctct
    46921 gaaacagtca catcaagtgc ctctgaacaa tcctacatcc taaccgaaga aaactgggac
    46981 agcattccag ccatgattgg gattacagac acagacctca aagagatttt tggaaaatag
    47041 tgcatcagaa aaaccaccaa ttcaggtggg ttttctgttt attaagatta ttttactgtt
    47101 gctgtgcttg taatcaattc aacagctttt ttgatagtga tatcatgttt caacatgtca
    47161 gctgaaagca agttttgaac ttgtgcaact tccatgttgt agtctgctgc caattgctca
    47221 acttcttttt ggatttcttc ttctgaagca tcaaatcctt cagctttggc aactgcttcg
    47281 ataacaaggt tagtcttagt acgtgactca gcttctgctt ggtattggtt gtgaaggtct
    47341 tcttgagtag ttccagtgat ttggaagtac atgtcagggt tgatcccttg acgttgcaag
    47401 ttcccaagga attcatttac tgaacggtga acttcttcgt ggatcatttc ttctggaagt
    47461 tctacgattt cagcattttc tacagctgta tcaattgctg caccttcaac tgcatctttg
    47521 taagcttctt ctttagcagt agccaattct ttgcggtatt tttctttcaa gtcagcaagt
    47581 gtttcaactt cttcatcaat gtcttttgca agttcatcgt caagagctgg aacttcttta
    47641 gcttttactt cgtggatagt tgtcacgaat ttagcttctt tacctgcaag gtcttctgct
    47701 tggtagtctt ctgggaatgt tacgataaca tcaacggttt cgccagctga gtgacctacc
    47761 aattggtctt cgaaaccagg gatgaattga cctgaaccaa gtccaagtga gaagttttca
    47821 ccttttccac cgtcaaattc aacaccgtcg atagaaccaa cgaagtcgat cacaacagtg
    47881 tcgccgtttt cagcagcagc ttccttgata accaattcag ccagattgtt gcgttcgcgt
    47941 tcgatacgct cttcaacatc agcgtcagtt acttcttttt ctacatcaac tgatacttca
    48001 aggtttttgt agtcacccaa ttttacttca ggttttgtaa cgacttcagc agtgataacc
    48061 cagtcttgac ctttttccat tgaagttacg tcaatttttg gttgggcaac cacttcaaga
    48121 ccagcttctt ttacagctgc ttcataagcg tttggcaaaa gagcgttcat aacgtcttgg
    48181 taaagtgatt cttcaccaaa ttttttatcg aagataggac gtggaaggtg acctttacgg
    48241 aaacctggaa cattaagaga tttcttcact gacttgaaga cacggtccaa ttctggtttg
    48301 atttggtctt gagagatagt gaaagtcaag acaccacggt ttgtttcttt gttttcaaat
    48361 gatacagaca ttctgtcatt tctccttaaa attttttaaa tacagtctat tataacattt
    48421 tctataaata aaatctagac taatgcagcc aattatagac aacaagcctt tttcctaact
    48481 aaaacactca taaagtatag acaattttag actactttgg atatcttttg gttagtcatt
    48541 ttccaaaact aatttatcta atttttcagt tatatagtag attgaaacta gaatagtaca
    48601 cctctacttc taaaacatt
//
LOCUS       DAVBCR010000005        48361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCR010000005 DAVBCR010000000
VERSION     DAVBCR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.005"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(859..2085)
                     /locus_tag="VQ508_000251"
     CDS             complement(859..2085)
                     /locus_tag="VQ508_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2489314.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2128..2820)
                     /locus_tag="VQ508_000252"
     CDS             complement(2128..2820)
                     /locus_tag="VQ508_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2489315.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(3006..4757)
                     /locus_tag="VQ508_000253"
     CDS             complement(3006..4757)
                     /locus_tag="VQ508_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489316.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4747..6492)
                     /locus_tag="VQ508_000254"
     CDS             complement(4747..6492)
                     /locus_tag="VQ508_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2489317.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGYDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6698..6994)
                     /locus_tag="VQ508_000255"
     CDS             complement(6698..6994)
                     /locus_tag="VQ508_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2489318.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(7147..7602)
                     /locus_tag="VQ508_000256"
                     /pseudo
     CDS             complement(7147..7602)
                     /locus_tag="VQ508_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7736..8563
                     /gene="xth"
                     /locus_tag="VQ508_000257"
     CDS             7736..8563
                     /gene="xth"
                     /locus_tag="VQ508_000257"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2489319.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8575..8718
                     /locus_tag="VQ508_000258"
     CDS             8575..8718
                     /locus_tag="VQ508_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2489320.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            8718..8957
                     /locus_tag="VQ508_000259"
     CDS             8718..8957
                     /locus_tag="VQ508_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2489321.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9341..9436
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9836..10417
                     /locus_tag="VQ508_000260"
     CDS             9836..10417
                     /locus_tag="VQ508_000260"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2489322.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10417..11679
                     /locus_tag="VQ508_000261"
     CDS             10417..11679
                     /locus_tag="VQ508_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2489323.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11766..12632)
                     /locus_tag="VQ508_000262"
     CDS             complement(11766..12632)
                     /locus_tag="VQ508_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2489324.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(12619..13389)
                     /locus_tag="VQ508_000263"
     CDS             complement(12619..13389)
                     /locus_tag="VQ508_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2489325.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILAPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(13415..14269)
                     /locus_tag="VQ508_000264"
     CDS             complement(13415..14269)
                     /locus_tag="VQ508_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2489326.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(14426..14830)
                     /locus_tag="VQ508_000265"
     CDS             complement(14426..14830)
                     /locus_tag="VQ508_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2489327.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(14900..16381)
                     /locus_tag="VQ508_000266"
     CDS             complement(14900..16381)
                     /locus_tag="VQ508_000266"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2489328.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(16400..17578)
                     /locus_tag="VQ508_000267"
     CDS             complement(16400..17578)
                     /locus_tag="VQ508_000267"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2489329.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            17743..18750
                     /gene="galR"
                     /locus_tag="VQ508_000268"
     CDS             17743..18750
                     /gene="galR"
                     /locus_tag="VQ508_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2489330.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(18796..19833)
                     /locus_tag="VQ508_000269"
     CDS             complement(18796..19833)
                     /locus_tag="VQ508_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2489331.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEVDDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATVVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(19834..20187)
                     /locus_tag="VQ508_000270"
     CDS             complement(19834..20187)
                     /locus_tag="VQ508_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2489332.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(20431..21321)
                     /locus_tag="VQ508_000271"
     CDS             complement(20431..21321)
                     /locus_tag="VQ508_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2489333.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            21454..21990
                     /locus_tag="VQ508_000272"
     CDS             21454..21990
                     /locus_tag="VQ508_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2489334.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(22157..22963)
                     /locus_tag="VQ508_000273"
     CDS             complement(22157..22963)
                     /locus_tag="VQ508_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2489335.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(23308..24828)
                     /locus_tag="VQ508_000274"
     CDS             complement(23308..24828)
                     /locus_tag="VQ508_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2489336.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(24821..25549)
                     /locus_tag="VQ508_000275"
     CDS             complement(24821..25549)
                     /locus_tag="VQ508_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489337.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(25564..26328)
                     /locus_tag="VQ508_000276"
     CDS             complement(25564..26328)
                     /locus_tag="VQ508_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2489338.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(26332..26775)
                     /locus_tag="VQ508_000277"
     CDS             complement(26332..26775)
                     /locus_tag="VQ508_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2489339.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(27457..27753)
                     /locus_tag="VQ508_000278"
     CDS             complement(27457..27753)
                     /locus_tag="VQ508_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2489340.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(27775..28839)
                     /gene="pepA"
                     /locus_tag="VQ508_000279"
     CDS             complement(27775..28839)
                     /gene="pepA"
                     /locus_tag="VQ508_000279"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2489341.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            29240..29941
                     /gene="galE"
                     /locus_tag="VQ508_000280"
     CDS             29240..29941
                     /gene="galE"
                     /locus_tag="VQ508_000280"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2489342.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            30364..31323
                     /locus_tag="VQ508_000281"
     CDS             30364..31323
                     /locus_tag="VQ508_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489343.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            31313..32269
                     /locus_tag="VQ508_000282"
     CDS             31313..32269
                     /locus_tag="VQ508_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2489344.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            32266..33018
                     /locus_tag="VQ508_000283"
     CDS             32266..33018
                     /locus_tag="VQ508_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489345.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            33114..34079
                     /locus_tag="VQ508_000284"
     CDS             33114..34079
                     /locus_tag="VQ508_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2489346.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34304..34546)
                     /gene="yidD"
                     /locus_tag="VQ508_000285"
     CDS             complement(34304..34546)
                     /gene="yidD"
                     /locus_tag="VQ508_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2489347.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(34546..35268)
                     /locus_tag="VQ508_000286"
     CDS             complement(34546..35268)
                     /locus_tag="VQ508_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2489348.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(35255..35824)
                     /locus_tag="VQ508_000287"
     CDS             complement(35255..35824)
                     /locus_tag="VQ508_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2489349.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(35843..36571)
                     /locus_tag="VQ508_000288"
     CDS             complement(35843..36571)
                     /locus_tag="VQ508_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2489350.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(36571..37311)
                     /gene="xerD"
                     /locus_tag="VQ508_000289"
     CDS             complement(36571..37311)
                     /gene="xerD"
                     /locus_tag="VQ508_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2489351.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(37302..37763)
                     /locus_tag="VQ508_000290"
     CDS             complement(37302..37763)
                     /locus_tag="VQ508_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2489352.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(37760..38281)
                     /locus_tag="VQ508_000291"
     CDS             complement(37760..38281)
                     /locus_tag="VQ508_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2489353.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(38257..39228)
                     /locus_tag="VQ508_000292"
     CDS             complement(38257..39228)
                     /locus_tag="VQ508_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2489354.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(39225..40019)
                     /gene="racE"
                     /locus_tag="VQ508_000293"
     CDS             complement(39225..40019)
                     /gene="racE"
                     /locus_tag="VQ508_000293"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2489355.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            complement(40226..40474)
                     /locus_tag="VQ508_000294"
     CDS             complement(40226..40474)
                     /locus_tag="VQ508_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2489356.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(40616..42241)
                     /gene="treC"
                     /locus_tag="VQ508_000295"
     CDS             complement(40616..42241)
                     /gene="treC"
                     /locus_tag="VQ508_000295"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2489357.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(42419..44386)
                     /gene="treP"
                     /locus_tag="VQ508_000296"
     CDS             complement(42419..44386)
                     /gene="treP"
                     /locus_tag="VQ508_000296"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2489358.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            44571..45281
                     /gene="treR"
                     /locus_tag="VQ508_000297"
     CDS             44571..45281
                     /gene="treR"
                     /locus_tag="VQ508_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2489359.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(45314..46117)
                     /locus_tag="VQ508_000298"
     CDS             complement(45314..46117)
                     /locus_tag="VQ508_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2489360.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(46130..46558)
                     /locus_tag="VQ508_000299"
     CDS             complement(46130..46558)
                     /locus_tag="VQ508_000299"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489361.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            complement(46567..47007)
                     /locus_tag="VQ508_000300"
     CDS             complement(46567..47007)
                     /locus_tag="VQ508_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489362.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            complement(47052..47957)
                     /locus_tag="VQ508_000301"
                     /pseudo
     CDS             complement(47052..47957)
                     /locus_tag="VQ508_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT        14190 a        11119 c         9060 g        13992 t
ORIGIN      
        1 ttcttatttc attttattat atctacaacc tcaaagcagg gttttgagct atctatggcc
       61 aacttcctag tttgctcttt agccttccat tcaaaagtga tgctatcttt cttagcttga
      121 atataaaata tcccatttac tatatttaaa tttgttgata tgttgtaaat tataaatgaa
      181 agaacaaata tcttgtcata caagcattcg ccagggatat ttacatgaat atcgctcata
      241 tggcggattg aaactaaaac actacaatat atttcttaaa tagaccttct ataaaagcta
      301 gatttaacct cgataaaaat agatgcagtg cttctaaaat actggaatag ggcatatagg
      361 taatcgtaga caagtgtttt atagattgtc tcgcgggaga tctaatgatg tatagcaagc
      421 atcactaaac atatagaata tttgtgttat tataatgtca atatgctata aattctatca
      481 tgcaaaagaa tgactagaac atgaaagaca ggcacaaaac tttgaacaat attctgaaaa
      541 caaaaaaaga cgcgagtttc cacaatctca ctcatggaaa ctcacgtctt tcaaaaaata
      601 gggtgaatta aaaactagaa caagacacta gattttctaa atgatgtcta gtaaggattc
      661 taaatgctac accaaattgc tcacatctta tttcaaacca cgggactccc acaagaaagg
      721 aaaatcctat aaaaattctc tctctgagga gagagaggcc tcaactacag gtgtaaacta
      781 ttatcaattg aaaacaacag tttttacact caattagact ttttagggac aggatcactt
      841 tcgcttgact gtagtttgtc atttttttga taaagttagc actttttcag aaactgtttt
      901 gaaagtctca atgatatagt ctacttcttc atcgcttaat ttagtatgaa gagggagggt
      961 aatttcattc tcaaagaagg cataggcctt aggatagttc gtcatatcaa atccaagatt
     1021 cttataggct gtcaagagag gaagcggttt gtagtgaaca ttacttgcaa ttcctgcttt
     1081 agccaattct tggatgatga ggttgcgttc ttctaggctt gctccttcta cacgggtgat
     1141 gtagaggtgg cgtgaagatt cgacagtttc agtcttgtgt gccaaaggat ggatgcgaga
     1201 acctgcaaaa ccactatcat agcggtccac aatgtcctta cggcgttgca acaaacttgg
     1261 atagcggtcc aattgtacca aaccaagtga agccatgata tcggtcatgt tgcacttata
     1321 ggctggtgta acgatatcgt attcccatga ccccagttgc atcttggcaa gagcatcctt
     1381 agtttgcccg tgaagggaaa ggatttggaa ttccttgtac atctcttcgt catcaatcac
     1441 tggattggct ttccaagtcg cacttccacc ttctgccgtt gtaaagttct taacggcatg
     1501 gaatgagaag gaagtaaagt cagcgataga accagaaggt tgtcctttat aagtagatcc
     1561 caaagcgtgg gcactatcag agacaatgac aatacggtta aaggcctttt gccacttgct
     1621 tgaagcggta aagaagtcac gttttttctc cacgacttgg aacaaacggt cataatcgca
     1681 aacaatccct gcgagctcta ctgggataat caccttagtt ttctcagtga tagcttgctc
     1741 aagcaggtca tagtccatct caaacgtatc tgcttggata tccaccatga caggggttgc
     1801 tcccacgtgc gtaatgacac tacatgaagc cgtataggtt atggctggaa cgatgacttc
     1861 atcaccaggt cccacttcaa aaacgcgtaa aatcaactcc agagcggctg tcgcagagtt
     1921 gagacaaaca gtcttaggtg tctgtgtgta aagagacaag cggcgctcca gttcttttgt
     1981 tttaggacct gttgtgatcc aaccagaacg cagggtatcc gctacttcag caatttctgc
     2041 ttctgtgata tcaggcggtg aaaatggaat attgtaattt ggcattgatt tgctcctttt
     2101 atctgtactc attctcttat gactacttta ttttagtacc ttaaacacgg tttgaaacat
     2161 gattttgatg tccccaaaga aactaaactc tcggagatag gcgaggttat agcgcatctt
     2221 ttcaggaaga acatgctcca cataggcctg atcaactgac agacctttct ctgtcatttg
     2281 actgatgatg gtgtcctcat ccttgtagtt gatgctggct ggagaggtaa ttcctgcttg
     2341 caagagcaag gttgccatca tttcagtgct atactgctct gtataacgtg gcacttcagg
     2401 tcgtgtaccg acaaaggaca tctcaccttt aaggacattg accaactgag gcagttcgtc
     2461 caaacggaca cgtcggatga aatttccaac cttggtaata cggctatcgt tagcagaagt
     2521 caccagattt ccttttttat ccgcatccgt caccatggta cggaacttcc aaatcttgaa
     2581 ccgacggttg tactgggtca cacgctcttg cttgtaaatc actggcccct tgctatccaa
     2641 cttgatccaa atgctcaaga tgagaaagat gggagaggtc agaaccagta agaccaaggc
     2701 caaaacccag tccaagcaac gcttgaaaat cagcgaaccc ttccttttag agacaagctg
     2761 gtagtaagac tcaacctcgc ttgatttcat ttccacaggc aagtcttccc atttcagcat
     2821 gctgttctcc tttgttttta ttatactatt tgtcaacact ttttgtcaac acttttcatt
     2881 ataccacaaa atggaaaagg cggtgaaaaa aatctgtaat ttgaaggatt tccttctagt
     2941 actcaatgaa aatcaaagta cagctttgag attgcagata aagctgacat ggtttaaaga
     3001 gattttcaaa gagtataggc catggcccct gcctgcaaac tatacatctt gtgataggtt
     3061 cctcccagag ccaagagttc ctcatgggtt ccactctcga taatgcgtcc cttatccaag
     3121 acatagatgc agttggcgtc ttgaatagta gaaaggcggt gagctatagc aatagttgtt
     3181 cggccctgtc tcatcttcgc cagagaagct tgaaccaagc tttctgtttc agagtcaata
     3241 ttggctgtcg cttcatccaa aatcaggatt ttaggctggc tggcgactgt tctagcaaag
     3301 gcaagaagct ggcgttgccc agtagagaag ctcgaaccac gctcggaaac aggggagtcg
     3361 tacccctgag gaagttcttg aataaaggaa tctgcatcca caaaggctgc cgcagcctga
     3421 acctgctcat cactgatttc ttggtacatg gcgatattgg acttaatagt tccatgatag
     3481 aggaagggtt cctgcaagac caaaccgatg ttttttctca gctcttcttg actgaaatcc
     3541 ctgatatcca catcatccaa gagaactctc cctgactgga attcataaaa gcgcatgagg
     3601 acattgataa tagacgattt ccctgaacct gtatgaccta caaaggcaat ggtttcaccc
     3661 ttattaacag agaaagaaat gtcatccaga atcggatgtt taccgtcata tgagaaacac
     3721 acatgttcaa agcggatatt gccttcttgg actttggctt gcccattttc ttgaagaggt
     3781 tcataggtcc tctcgtctac cagggcaaag acacgacctg cagaaaccat agacgtttgc
     3841 agtgttgaaa agttttgcgt cacctcaatc aaagggtcaa agaggcggtt gatgtactgg
     3901 ataaaagcat acatggtccc ggccgttatc ccgatagaaa gaccacgata gccaaaatag
     3961 gccatcaaga cagcataacc tagaagtttc aacaaactca tggcaggtct caaaaagagg
     4021 gcatccaagg ctacagaacg gttggcatag accaagtgct cttggttgat ttcatcaaat
     4081 tctgcctgca ggcgcttctc ttgattaaag gcctggatta ttctgattcc ctcgatgttt
     4141 tcagccagct tgctattgat atctgacaag agacttctgg ttttctcaat gattttcact
     4201 gattttttcc gatagagatt gaccaaaagg aaaatcaagg ggagaaagag caagactaat
     4261 gctgtcaaac gaaaatccaa taccaacatg gtataaaggg ttgtcagaaa gatgaaaact
     4321 gctgagataa agctagataa aatcccagaa aacatatcac taatcgtctc ggtatcgttg
     4381 gtcaaacgag aaacgataga acctgctggc gtcttgtcaa agtaagacat gcccagtttc
     4441 tccatattgg caaaggcatc ccgacgaata tccctaacaa tactgtaaga cacgcgcgca
     4501 aagagaagat tgccgacata ctgaactaca gtttgtagga tgtagagacc atagtagacc
     4561 agcaaaacgg taacggctag ttggttaaga ttgctgagat actggtcgat aaagtgggaa
     4621 gccacgaggg gtatgacact tttaatgacc gtcgtcgcta ggagaaaact gagtgccaaa
     4681 aaggtcagga gtctataagg cttgagataa gacatcaagc gcttcaatac agcccattgt
     4741 tcttgtttat tctgcatctt cttctccttt catttctaac tgctgagact ggtaggtttg
     4801 ggcataccag ccatccatcg ctagcaagtc ttcgtgcctg cctcgttcga taatttgacc
     4861 attttgtaga actaaaataa aatctgcatg gacaacagca ctgaggcgat gggcagttat
     4921 aatggttgtc ttgtcctttc gcgtctcctt gagattgtcg ataatcgcat actctgtctt
     4981 ggcatctacg gcggataagg aatcatccaa aatcaagata tcagggttta aaatcatagc
     5041 ccgactcata gccaaccgtt gcttttgacc accagaaaga ctgactcctt tttcaccaat
     5101 cagcgtatca aatccttgag gcatgtctac aatatcttgg taaactcggg ctagcttagt
     5161 agcttcctca acctctgaaa ggggcaagtt aggattgcca aagcggatat tgtctaggat
     5221 tgaagtcgca aagagaaact ggtcctgagg aacatagccc atgagactgc gaagttctgt
     5281 caaacgatag tcccgaatat cgtaaccgtt tagataaatg gcacccttat ccacatcgta
     5341 ttcacgcaag aggagcttga ttaaggacgt tttcccagag cctgtctgcc caaccaagcc
     5401 cagtgtttgc ccttttgcca aactaaagtg aatatccgtc agtgtttcct cattttcaaa
     5461 agcaaagctg tcaatggcat actccaaacg cccattttca ataccatcca gaggaaactc
     5521 agggtcttgt acaggagatt cctgagacaa aagattttca atccgctggt aagaaacctt
     5581 ccctcgctga gtagtattaa agaggaagcc gatggccata agaggccaga ccagcatatc
     5641 caaatagctg ataaaggtga ctagattacc aactgtaatc tgcccttcct gaaccatcaa
     5701 ggagccaacc aaaagcgtta aaacatagga cgaaccaaca aacaagagaa ccatagggtc
     5761 aaagagacta tcatatttca tggtttgcag gttcttttgg aaggttaatt cattgactgc
     5821 ctgaaaagac ttcaactcgt ctgcctgata accgaaagac ttggtcactt tgatacctga
     5881 tacggactcc tgtaccttgt tattgagttc agaaaaagca gcttgggatt cgccaaaggc
     5941 cttatgagtc tttctcccta ggcgactagt cgcataggcc atgaaaggta gggggagaat
     6001 ggcaacaaga gtcatctgcc atgagatgct aaagagcatg gtcaacaaag tcaccagagc
     6061 cgtgatagag gcatccaccg cagacatgac accgccacct gctaaacgag tcaaggcatt
     6121 gatatcattg gttgcgtgtg ccatcagatc acccgtccga taggtttgat aaaaggctgg
     6181 cgacattttt gtgaaatgct taaacaagcg agaccgcatg atctgtccca agcaataaga
     6241 ggtcccaagg atatacatac gccacacata gcgcaaatag tacataccaa aggctgcaag
     6301 tagcaagtaa aataggctaa gaaggaggtc ctgctgggtt aattgccccg atgtgatggc
     6361 atcaatgact cgccccataa ccataggagg aatgagattg agaacggaaa ccaagaccag
     6421 ggccacaatc ccgactagat aacggtgttt ttctaacttg aaaaaccacc agagtttttg
     6481 aataatggac ataaaatccc tttctgattg caaatggaaa cctgaggcca atactcaatg
     6541 aaaatcaaag agcaaactag gaaactagcc gcaggctgct caaagcactg ctttgaggtt
     6601 gtagatagaa ctgacgaagt cagtaaccta catacggcaa ggcgacgttg acgcggtttg
     6661 aagagatttt cgaagagtac aagaccccag gtttttctta ttcataagtt acgactgtaa
     6721 cagcaccctt gtcatattca gcaataaaga tattggctac attgtcatgc ccttgtttgc
     6781 tgaggttatc aagcaaccac tcttcgctac gaccaatcga ttccaagacg tctacttgaa
     6841 tcacaccgtc agtcacaact ggatacttag gattttcatc tcccatttgc acaacgatga
     6901 gttgcccatt ttgctcttgc acagctcgtt tgacttgctt catctggaaa atcccttggc
     6961 tacgaagttt gagggctaca tccgatgcag acaaaccaac tgaacgacag gcttatgggt
     7021 caatattccc atttttgata aggagagttg gcttaccatc aatcaaacgt ttcacaaagt
     7081 gaacattgtt gttgagctat ttgagagtca agatcagttt gattaaaatt tcgatataat
     7141 taagtgtcat ttgttcacat ccaccagttc aattgcgtct gtcttataca attcgacttt
     7201 ctctaacagg tacttgtctg gctcacttcc gtttagggca cgataatagc gatctcccac
     7261 cttgataagt gcgccatctg tgtttgtgga agtattaaca tggacttctg acttgtctac
     7321 tcccaaatct ttggaaacaa cctcgatgaa atgaagtgaa gtttgaaatt tattgttaga
     7381 aacttgattg ttttgataag tcgtgatgct gaccaaaagc atagctaaat aaggccagag
     7441 ctgaaatcat gaccaactca cgaaatttgg ttcctttttt atcatggtat gccttgaaag
     7501 caaaaaaact gtcacagcta atagaacaat cccaaagcca accatgatac cattttgctg
     7561 accgatttgg ctgagtacat agtcataaga gtagaatttc atgtattttc actccctttc
     7621 ataaaatcat tcttaattat aaacgaaaga gagtaatttt tcaaaccata cgttggggaa
     7681 atagatcttt ttttggtata ataaaatcta taatctgaat gaaaaaggta actttatgaa
     7741 acttatctca tggaatattg attccctaaa cgctgcccta actagtgact cagctcgtgc
     7801 caaattgtcc caagaagtcc tacaaacctt ggtcgctgaa aatgctgata tcattgctat
     7861 ccaagaaacc aagctttctg ccaaaggacc tacaaagaaa cacgtggaaa ttttagaaga
     7921 actcttccca ggctacgaaa acacgtggcg ctcttcccaa gagcctgccc gtaaaggcta
     7981 tgctggaacc atgttccttt ataagaaaga acttacaccc actatcagct tcccagaaat
     8041 cggtgcccct tctaccatgg acttggaagg ccgtatcatc actctagaat ttgatgcatt
     8101 tttcgtaacc caagtttaca ctccaaacgc tggtgacggt ctcaaacgct tggaagaacg
     8161 ccaagtctgg gatgtcaaat atgcggagta tttggctgaa ctagacaaag aaaaaccagt
     8221 ccttgcgacc ggtgactaca acgtagccca caatgaaatc gaccttgcaa atcctgctag
     8281 caaccgccgt tcacctggat ttactgacga ggaacgtgct ggatttacca acctattggc
     8341 aactggattt actgatacct tccgccatgt tcatggcgat gttcctgaac gctacacttg
     8401 gtgggcacaa cgcagcaaaa cttctaaaat caacaataca ggctggagaa tcgactactg
     8461 gctcacaagt aaccgtatcg ctgacaaggt cactaaatct gatatgattg actcaggtgc
     8521 tcgccaagac catacaccga ttgtcttgga aattgacttg taaggatttt ctatatggac
     8581 tacaatgcgg tcattcccga gtttcttgta tcgaatatcg aacagtcccg gtccttctac
     8641 tgtggtttac tgggttttag gattgaatac caacgttcag aagaaaactt tctctttctc
     8701 ttgaagagtg tcaactaatg ttagaggagg gaacaaagga tcaactagct gaactgacct
     8761 acccctttgg tcgtggagtc aatctctcct ttggcataaa ggatgttcca aaactctatc
     8821 aaaaagtgat ggaggcaaac tatcctattt atcgtccctt gaccaaaaga aagtttcgtg
     8881 tcagtgatcc ctatatctat cctcataaat ttgcagtttt ggatccagat ggctattttt
     8941 taagatttag cgagtagaat aataaaaatt gggaaaagtc tttaaaagtt agaaaaaggc
     9001 atattgtcag gtgtctaaaa acttgatagt atgcctttta tgatggaaaa atcttctaat
     9061 actcttcgaa aatctcttca agccacgtca acgtcgcctt accgtaggta tataatactg
     9121 actctgtcaa ttctatccac aacctcaaaa cagtgttttg aggttgtgga tagcttccta
     9181 gtttgctctt tgattttcat ttagtataaa tgttagctta caatggagtt tttgttcacc
     9241 ctctcttctg tttgttcgtt ttttaaacca aaacactaac aaaatagttg atttctaaag
     9301 ttatttagtg taaaataaaa acattggctc aagcctattt aaaattgaat atcgttttac
     9361 ttgtttatgt cgtgaattgg cacgacgttt ctacaaggtg ccggaacacc taacaataag
     9421 taagtcagca gtgagatatg gccgtttttg ccatgcctat ttgggggact tacttagaga
     9481 ttaagtaggt cttttttgaa tatttttaat atttccctta attagtgcgg acgggaggtg
     9541 aaattatcct gtgaataatt tcaggaatcc agggaatttc atccctaatt tttgagcccc
     9601 tagctctttc atttctaggc ttaggctaaa atagttcccc gaactatttt actctcaaaa
     9661 atcccgtcat agacaataac aagattattg tctatgatac cactacaacg ggaaatattt
     9721 gataagactc gcttcgctcg taatagtttt atcaaatata tagcattagc aaaagggatc
     9781 actatagaaa catttaaaca acaagtaaat taacttttag actttaggag gtcttatgaa
     9841 attattagaa gagcgcatcc tcaaggatgg gcatatcttg ggtgataaca tcctcaaggt
     9901 agattccttt ttaacccacc aagttgactt tagcttgatg cgagagattg gtaaggtttt
     9961 tgcggaaaaa tttgctgctg ccggcattac caaggtcgta accattgaag cgtcgggtat
    10021 tgccccagcc gtttttacag ctgaagcctt aaacgttccc atgattttcg ccaaaaaagc
    10081 taagaacatc accatgaacg aaggcatctt aactgctcaa gtctactcct ttaccaagca
    10141 ggtgaccagc accgtttcta tcgctggaaa attcctctca ccagaggaca aggttttgat
    10201 tatcgacgat ttccttgcta atggccaagc tgctaaaggc ttgattcaaa tcatcgaaca
    10261 ggccggtgcc acagtccaag ctatcggtat cgtgattgag aaatccttcc aagatggccg
    10321 tgatttgctt gaaaaagcag gctaccctgt cctatcactt gctcgtttgg atcgttttga
    10381 aaatggtcag gtcgtattta aggaggcaga tctctaatgc aaactcaaga aaaacactcg
    10441 caagcagccg ttcttggctt gcagcactta ctagccatgt actcaggatc tatcctggtt
    10501 cccatcatga ttgcgacagc ccttggctat tcagctgagc agttgaccta cctgatttct
    10561 acagatatct tcatgtgtgg ggtggcaacc ttcctccaac tccaactcaa caaatacttt
    10621 gggattggac tcccagtcgt tcttggagtt gcattccagt cggtcgctcc cttgattatg
    10681 attgggcaaa gccatggtag tggcgctatg tttggtgccc ttatcgcatc tgggatttac
    10741 gtggttcttg tttcaggcat cttctcaaaa gtggccaatc tcttcccctc tatcgtaaca
    10801 ggatctgtta ttaccacgat tggtttaacc ttgatccctg tcgctattgg aaatatggga
    10861 aataacgttc cagagccaac tggtcaaagt ctcttgcttt cagctattac tgttctgatt
    10921 atcctcttga tcaacatctt taccaaagga tttatcaagt ctatctctat tttgattggt
    10981 ctggttgttg gaactgccat tgctgctact atgggcttgg tggacttctc tcctgttgcg
    11041 gtagctccac ttgtccatgt cccaactcca ctctactttg ggatgccaac ctttgaaatc
    11101 tcatctattg tcatgatgtg tatcatcgca acggtgtcta tggttgagtc aactggtgtt
    11161 tatctagcct tgtctgatat cacaaaagat ccaatcgaca gcacgcgcct tcgcaacggt
    11221 taccgcgcag aaggtttggc cgtacttctc ggaggaatct ttaacacctt cccttacacc
    11281 ggattttcac aaaacgttgg tttggttaaa ttgtcaggca tcaaaaaacg cctgccaatc
    11341 tactacgcag ctggtttcct ggttctcctt ggactgcttc ctaagtttgg tgcccttgcc
    11401 caaatcattc caagctccgt cctcggcggt gccatgctgg tgatgtttgg ttttgtatct
    11461 attcaaggga tgcaaatcct agcccgagtt gactttgcta acaatgaaca caacttcctt
    11521 atcgcagctg tttcaatcgc tgcaggtgtc ggtctcaaca acagtaatct ctttgtcagc
    11581 atgccgacag ccttccaaat gttcttctca aacggaatcg tcgtagccag cctactcgcc
    11641 attgtcctca atgccgtatt aaatcataaa aagaaataag aaaaagaggt gcgagcctct
    11701 tttttgttta tcacaaattg attataaata caatcaatcc caattcccat ctaattgaat
    11761 tggacctatt tcaaattctt taaaatatta tgttccaaat cgttaatatt atataagaat
    11821 ggtaaaatat caaatgtttc tcgtaaatta ttcttagcct tataccaacc ttgtccatca
    11881 gtaatccaca taaactcaac attaggaata gctttaattt cttcagcaat cattttgtaa
    11941 gatctagccg tctcatttaa ttttgaacca cttcctgaat aaaaattaac ctcaatcaga
    12001 taaagcactt gttcattttt tatgacaaaa tcaaaacgtt tcacagtatt cccatcattc
    12061 gtaatagcag ataaatcgac agaaaaaatt tcctctattt cattttgttc aatctcttta
    12121 aataaattct ctcctaaaat atacccttca gcttcaagat aactttgaac aatattttcc
    12181 atcgcatctc cagtccgatt tttgcgccca ttagtatcca taccaacctc aacacctgta
    12241 acgtagtcaa ctaaatttga tactaaatga ttttgcaata aatcaaatat acctgatttt
    12301 tccaaaaaca tagtatattc ttcaatacta tagtttcgtt tactaaaatc aaaatagaaa
    12361 tcttttatcg gatccttaac aataatcgta tctctcaaac gtttagcaat aagtagaggc
    12421 acaactttca aaatttctgg ataattttga tataagtcta aaaaatcctc ttgtatattt
    12481 ttactaccaa taagactatt cataatattt agagcaactt ttatactaga tacattttta
    12541 taaaccttgg gaaaatctgt ataataagtc cagtcagcta ctgtgtcggt catcgttgac
    12601 aaccactcat caaaatttct agtttgtttc attttctgcc tccaacctct tccttgcaat
    12661 ttttaaatat tctttttcag catcgatacc tataaatctt ctacctaacc gcttcgcaac
    12721 aacacccgta gtgccactac caacaaatgg ggctagaata tagtcaccct ctttagtaga
    12781 ggctaaaata atgcgttcta acaaatactc tggtttttga gttggatgtt tcccagccca
    12841 tttttcaact ttctttgtta aagaacccgt ccagacatct ttcatttgtt ttccatcatt
    12901 caattctttc attaaatcat aattgtagta atgacgagct tttttatcat tttttctggc
    12961 ccataaaatg gtttcagtag aatgggtaaa ataacgacaa gataaattgg gggcagggtt
    13021 tgttttctgc caagtaatat tattcagaat tttaaaacct tcttgttcta atgccattcc
    13081 aactgagtat atgttgtgca aactacctga aatccatacc gtcccattag gcttcagaac
    13141 ttcttttgct aggcgaatcc atttacgatt aaactcatgt ttttcttcga atgaagaaat
    13201 tttatcccaa tctcctttat caacagaaac aacttgtccc ccagaattag atattccacc
    13261 attacttaaa aaataaggtg gatcagcaaa aatcatatcc atactttctg gtttcatttt
    13321 tgataagaac ttgaacgtat ctgaatgaac aagaatcatc ttatttttat tgtagtatgg
    13381 ttttgtcata agaacacctc catacttata ttcgttattt ttcataattt gtgactataa
    13441 tttcagaaat ttttcctcga cttgaagatt ttgctccatt agttcgggta gcttcaacat
    13501 aatgtatatt aaaatcctta tacaactcct ctactaaagc actagaagaa tttgataaca
    13561 taacataagc tcctgtatca ctcaatctct taaaggcatc tcttaatctt acttgatctg
    13621 caaaagagaa tccctcatga gtataagacg taaatgcact cgtctcagac aatggaatat
    13681 atggagggtc aaaatacaca aaatctcctg ttcgaacatc tacaattgcc ttttcaaaat
    13741 ctcccacttt aatttctagt tgattgttat ttagataaac tgaaatagca gatatcaatt
    13801 cctcatcaac aattttagga ttcttataac gtccatatgg aacattaaat tggttcttag
    13861 aattcacacg atatagacca ttaaagttca ctctcaacat atatagaata cgtgcagctc
    13921 tttgtacttc ggacatcata tctattcttt catcacgatc tgcagaacgt aaatctaaat
    13981 aatattcttt tgaattatat tcctgatgaa ctttcaaaat ttcaatcaat tcttgaggat
    14041 tgtccttaat ttgttgatag caatttatta gttcagcgtt aaaatcatta ataactgcat
    14101 ctttaggagc caaatcaaaa aataaagctc cacctccaac aaaaggttcg aaatacctgt
    14161 tataggtttt aggcattaat tctctaataa caggcaataa ttgtctttta ccacctgtcc
    14221 atttcgtgaa cggttgtaaa gtaactttct ttatttcttt tatcttcatg cttttaatta
    14281 taccatagaa attaaaacac aactcttgta ttttctacca cattactaaa ttttctattt
    14341 tagaattaaa tcatatcaat ataaaaaaat gagtaaggca aatcttataa attccaaaat
    14401 tcacctcact ctattaaatt actctttata tccttacccg tctttcctca gacctttggc
    14461 ctgtgacaaa catagtaaag gttgctttgc agacatttct gccctcttga ttggtgatat
    14521 ccacatccat cacacaggtt gtacgacctt gatggacaca ttctccttta atggtcaata
    14581 catcgtcgag ttttcctgcc ttaaggtagt tgatagagga ttggagtgtc accccatcaa
    14641 gtcccagcga gataaccacc aaaccactga tttggtcaca tagtgtgaat agatagccac
    14701 catgggcatt gccatagtag ttgagcgacg agttcactac tttggtcgtc accacaacgt
    14761 gaccatctct cattttttca atttcgtaat tttcaaaggc agatatagcg tcaaaatgaa
    14821 agtctttcat cgtttcctcc tgatatttca aatgacacta tgatactact ttttatagga
    14881 ctgtgcaagg agaaagctcc tagtctagta aaattccgac atgttccaca aagcgcataa
    14941 aggctgtctg cccttgttct gtctgcttgt agactcctgc atcctcaagt acacgcgcaa
    15001 agatagcacc cacagagtcc ttgacgattg caagggcttt ttctttatcc gttaggtctg
    15061 gatgttggga tttgagttgg tctgcccact cctgatgata atcggcaact gtaacagctt
    15121 ctcctacaag atagctagcg acttgctcta cttcttcttt cagacgtggt ggcaagattg
    15181 ccaagcccat gacctcaatc aagccgatat tttccttctt gatatgttgg acatccttgt
    15241 ggggatgata gatgccatca ggatgctctg ctgaagtctg attgtctcgc aagaccaagt
    15301 ccaactcaaa ctgtccatca cgcttacgcg ctattggtgt gatggtgtga tgcggtgtcc
    15361 cgtctgtctc tgctaaaatc tgcacactag aatctgaata ctgacgccat tcctggaaaa
    15421 tcttatcagc taagttaatc aaatcctctt tggaatccga agtcaagcgt aggacagaca
    15481 ttggccactt gataattcca gccttgacct gctcaaaacc agcaaatcgg aaggtctttt
    15541 gcaagggagc caattccata ggaaatacgt gacggcctcc ctgataatga tcatgagtta
    15601 gaatagagcc ccccacaatc ggcaggtcgg cattagatcc agcaaaatat cctggaaact
    15661 gctctacgat agccaacaga cgttcaaaac tctgacgact aatggccatg ggacgatgct
    15721 ggccatctaa aaagatacaa tgctcattaa agtaagcata gggcgaatac tggaaacccc
    15781 attcctgacc aaccatttca aaacggataa tccggtggtt gctgcgagct gggtggttga
    15841 cccgaccatg gtaaccctca ttctctaaac aaagttgaca ctgaggataa ttactagctt
    15901 gcaccaactt ggctaccgca atctctttcg gatccttttc aggcttagag agattgatgg
    15961 taatttcaag ttctccataa tcagatggaa cacgataagc gatattttta gcaatggcct
    16021 tgagtttgat gtagtcattt ttctgactga gttgataaaa atcctctatc gcttgctcag
    16081 gagagtaggc ataggcttcc cagaagtcac gattgacctg actcggacaa ggagtcacca
    16141 agtccatcaa ttcagtacca aggatttcac gcgcagtctg actatcctca atcgtctcta
    16201 atcgaacggc ttcctcaact agctggtcct tgaggtcaat caatttatcc agattggtct
    16261 caacttccaa aacaccttct cccactcgtg ccaaaacacg attggtcagg tagattcgat
    16321 ccatttcctc aaatgaactt tcagaaatga catgtgttac aaatttatct actaaggtca
    16381 ctatagagcc tccttttgac tagtcaagga cgcgagtgcc acctgcaact tcagcgatat
    16441 agaagcttgg agcgtatcca actacttcct cgtagtgttt gcctacagct tccttaaagg
    16501 cctcaacagt atctttttga accaaggcaa tggcacagcc accaaaacca gcccctgtca
    16561 tacgagcacc gagaactcct tcttgtgccc aagctgtgtg aacaagagta tccaattcca
    16621 aaccagttac ttcataatca tgctccagag aaacgtgtga cgcattcatc aaacgaccaa
    16681 atgtttccaa atcacctgct tgaagggctg cttgagcttt aagggtacgt tggttttcaa
    16741 acacagcatg gcgagcacgt ttcaaacgat tttcatcttt aatcagatag ctatattggt
    16801 caacggccca ctcgtccaat tcacccaggg tctgaatatc caaggcaact tgcaattctt
    16861 ccactgcttt ttcacactca gcacgacgtt cattgtattt agagtccgcc aattcacggc
    16921 gtttgttggt gttcatgata acaacgacat tgtccttcaa atcaagtggc accaagtcgt
    16981 attctaaagt attggtatct aagtaaatag cacgttggtc agcccccata ccaatagcaa
    17041 attggtccat gataccagag ttgactccga taaagttatt ttctgtttgt tttccgattt
    17101 taaccaaatc gagacgctct agttttaaat caaagagatg ctctgccacg actcctgtca
    17161 agagctccaa ggatgctgaa gaagacaaac cagcaccatt tgggatattc ccataaacat
    17221 aaaaatcaaa acctttgtca atcacgtgcc cagcttcttg caagaaatga aggacacctt
    17281 ttggataatt ggtccagttg tgctcttttt caaacttgag gtcagcgaga ggcacttcga
    17341 taatgccctt gtcctcaaag ttagctgagt agaaacgcaa gacttggtcg tcacgcttac
    17401 gagctgcacc gtaagttccc aaggaaatag cagcaggaaa aacgtgccca ccgttgtagt
    17461 ctgtgtgttc accaatcaaa ttaatgcggc ctggtgaaaa gaaggtttgg tctgcttctt
    17521 gaccaaaaac agcaagaaag tctttgcgaa gggcttcagt agtaagatgt tgtgccatat
    17581 gaattctcct ttgactgtcc gttaagccac cttaacgtat aatcgttttc tttctattgt
    17641 atccaaggaa ttttccaaga tcaatccttt ttactaaaat tttagtaaaa tatcagtaaa
    17701 aagaagaaaa acatgatata ctaacctaat caaggaggat ttatggctac cttaaaagac
    17761 attgcacagc tagcctctgt ctctatcgcg accgtatccc gcgtcctcaa tcgcgaccag
    17821 agcctatctg ttacagaaga aaccagacac cgtattttaa ccgttgctga agagctgggc
    17881 tacaccaagc acctcaagac aggcgagtcc cacaaaccca agcaaaagat tgccattatc
    17941 caatgggtca gcgaacaagg ggagctggac gacctctact actaccagat tcgcctagga
    18001 atagaaaaaa gagcccaaga gttggactat gatatcttgc gctattttaa tgaccatcct
    18061 tttaccttaa gcgaggaagt gattgggatt ctctgcattg gaaaatttag ccgagctcag
    18121 atttctgcct ttgaagaata ccaaaaaccc ctggtctttc tagacagtga tacactttct
    18181 ctggggcata cttgtattat tacggatttt tacactgcca tgaaacaggt tgtcgattat
    18241 ttcctcagcc aaggaatgga ccgtatcggg attctaacag gccttgaaga aacaactgac
    18301 caagaagaaa tcattcagga taagcggctg gaaaatttca gaaactacag tcaagcaaaa
    18361 ggaatctatc atgatgaact ggtctttcaa ggaaacttta ctgcccagtc tggctatgac
    18421 ttgatgaagg aagccattca gaacttggga gaccaactcc caccagcatt tttcgcagcc
    18481 agcgatagtt tagctatcgg tgcccttcgt gccctccaag aagctggaat cagcctacca
    18541 gaccgtgtca gcctcatttc ctttaacgac accagcctga ccaagcaggt ttatcctccc
    18601 ctctccagca tcacagttta tactgaagaa atggggcgag caggtatgga tattcttaac
    18661 aaggaagtcc tccacgatcg caaaattcct agcctgacca tgctgggaac cagactgaca
    18721 ttaagagaaa gtaccctaaa tcaagaatag gataacataa aaaacgaata gagttctaaa
    18781 actcctattc gttttttatt cgattacaat catagactta atggtcttac gttcatccat
    18841 atctttgtag gcttggtcga tatcttccag tttataactt gaagtaaaga cgcgacctgg
    18901 attgatatca ccatcaagga cggcttttag taaaaattgc ttatcgtatg ttgtagcaga
    18961 agctgcccca cctgctacag agatattttg cataaatgtc gaaccaagag cacgattatt
    19021 atagtgtggg actcctacaa agcccatacg ccctccatta tgaagaacac ctagcgcctg
    19081 ttctatagca gcctccgtac caacacattc aagtgctgcg tctgctcctc caccgaggat
    19141 ttcacgcacc ttggtaattc cttcttgacc acgttctgca acaacaactg tcgcacctga
    19201 ctccatagcc atcttttgac ggtcttcatg acggctcata aggataattt gtgatgctcc
    19261 acgcatctta gccgcgatga cagcacattg accaacagcc ccatcaccga taacaacaac
    19321 cttgtcccct ttttgaacat ttgcaacacg cgccgcatga tagcctgtcg gcatgacatc
    19381 tgcaagagtc aaaagggact tgagcatccc ttctgtatag tcagaaggtt gaccagggat
    19441 tttaaccagc gcccagtttg catagtggaa gcgaatatat tctgcctgaa aatcaccccc
    19501 caaattattg ccaatatgat tgtcgcaaga accgtcaaat ccagcaagac aggcatcaca
    19561 ctcaccacat ccatgtgtaa aagggacaat cacaaaatca cctggtttca ccgtcgtaat
    19621 ggcttcccca gcttcttcaa caatcccaat cgcttcgtgt ccactatttt tgtgtccagc
    19681 tttcgtttct ggattacggt acctccataa atctgaacca caaacgcacg cacgaaccac
    19741 acgaataatc acatcatcca cttctattat ttgcggacgt tcaatgctag caagtccaac
    19801 ctgacctgcc tttgtatata ctgctgattt catttaaaat tttccttcct tataaagttt
    19861 aattttgaga tttaaacgat ttaaagctgt ctgtagctga gacaagcgct cctctaattt
    19921 ttgcttttcc tcttctaaaa taccaagcct ctcctctctc gtttcatctc ccttttggta
    19981 gagcgacata tagtcaacta aactatctac agatacaccc gccgaacgaa aacacttaat
    20041 aaattccagc gcttcgatat cctgatcttg aaaatcacga atcccagtag cagtacgagt
    20101 aatcggtggc acaagaccaa cccgttcata ataccgaatc gtatccgctg aaattcccaa
    20161 caaatcactg gcagatttaa tattcacacg ctaacctcct ctcatttcat tcactgaatt
    20221 cacgctattc taactcactt atcttattat ataactttga gttgactcca agtcaagcat
    20281 tttgtatcta atcgaaaaat ttttatcatt aacaatcata tatccggagt ttatacaatg
    20341 ctcttctctt accaattttt gaaatattta aataataaag aaattactct attccaaata
    20401 agatagtacc caagtatttt tcactttttt ctaatgttga tgctcataac tccgttccaa
    20461 gtcacacacc tttcgcttat gggtttggtg agtttctagg tcagcatcaa tttcaatggt
    20521 aatattttga aaaccacaat cttttaggaa aattcgaata gactctttac aagtttccat
    20581 atgttccatt tcttttagac aaacatggac aatggcattt ttttccaaag catccatagt
    20641 ccagagatta agctgattaa ggctggccac attgtccaat cgctccaggc cactctttac
    20701 ttgcttgata tcaagacctt ctggcacagc atccaagaaa atcttgagtg tagaccaaaa
    20761 acgtggaagg gcttttgaaa gaataaagaa agaaatgaca agggacaaaa gaggatctag
    20821 gatataccag tccgtaaatc gaagaacaat cgccatcagg ataacagcta cccaccctag
    20881 cgtatcttcc agaaaatgca gactcagaat agactcattc tttgtctttc ccttaccaac
    20941 caccagactc gctaacagat tgatagtaat cgcaataatt cctaaccaga gaatcccctc
    21001 atcattgact ggttgcggat gcaaaatctt cgtgacattt tccaaaatga ctagaacaga
    21061 gcccgttacg agaatcacag ctgttaccaa ggctcctagc aggctaaacc gcttatagcc
    21121 caaggtgtac tgattgtctt cttcacgatt ggagattgtt tctagaaaag ctgatattcc
    21181 aattgcaatc gcatctccca agtcatgcac agagtcagca agaacagcgc tagaaccaaa
    21241 tactccacct gcaataaact caacaatggc ataagtcaaa tttaagaaaa aagccaccca
    21301 aacagcatat tttgccttca tatttctcat tcctttgtta taatagattt atgaacacct
    21361 tgttcattat cattatccac taaaactcaa agaaaggata gaagcaaaac gtcagcttta
    21421 ttcagttctg aacaatttgc ctcaagtgtc catatgacta acattgaccg ccgtatcagc
    21481 aaaaccaaaa aagccatcta tcaagctttt atacaacttt tgaatgctaa gggctacgag
    21541 gccactactg ttcaggatat cattgatctc gcagatgtgg gacgatccac cttttactgt
    21601 cactatgaga gtaaggagct tcttctggac cagctctgcc gctacctctt ccatcatctt
    21661 tttgaaagag agcaagccat ttcaaccgaa gattacctag cccacctctt tctgcatttt
    21721 cagaaaaacc aagaccatat caccagtcta ctattctcca aaaatgacta tttcctccgt
    21781 caactacaga gagagcttga gcaccacgtc tatcccatgc tggctgctga tttgaagcaa
    21841 actcacccaa gtctacctac ttcttacctc caacacttgg tcgtgtccaa ctttatcgag
    21901 acattgacct ggtggctcaa aaaaggtcaa gatttcacag accaggaagt tgtccaattt
    21961 tatctagacc ttctcattcc taaaaattga atatagagta aagcttcagt tgtcttattt
    22021 ctaggttact gagtttttta tcttttcaac aacaaaagag gacccgccga tcctcttttt
    22081 catactataa atccttgatt atcaattata tctgttttaa tcgaaatctc aaaacagcac
    22141 tttcaaacat cttttcctag ttaagtaaat cagtattctg cttagctgcc ttgctccatt
    22201 gataccaacc aactagactg ttaatgagat aaattagata tttcccttga atttgcaggc
    22261 tttctcccca ccagagatag attgaaaaga cattggtagc cgcccagaat atccactgtt
    22321 cacggtaaac agctgtcatg aggatttgcc ctaccccatt ggttgcatct gtgattgaat
    22381 cacgataggg acgattggca ccaatagact gataaatgaa gccaaaggcc aaccaccaaa
    22441 gcacactaat ggaaagatac tttgtccagc ccttgccgtc cagtttacgc gcgacaaact
    22501 cctgcttttc cttcttaaac tgtgcctgat aaatccaaac tagaagtcca attggctgca
    22561 tgactgtgaa gtaaagtgtc gtcagcacct caccataaaa gcctttctgt agggccaaaa
    22621 taaggtaaat aacagagtta atcaagccaa aaagataatt acttgctcga ccttccgata
    22681 caaagattac acagataatc cctgtcaagc tacaaatcat cccaatccag tcaacaatac
    22741 gatgttcgta aaccaactcc agccagagag gaaaacttcc taaaaccagc aaataaatcc
    22801 actgggcaaa actacgatgg gcaaagaggt catcccagat agccttcata gttcctgaaa
    22861 atcctaaatc agccatagcc gcaaccatac gacggtaacc acctgacatt tcacctaggg
    22921 ttgttttgat attttcaatt ttcttttgca aataagtatg catcatttct ccttttgttt
    22981 ttaaagagcc gtgtctggat agactttcgg acgcaacgct ctattagata atgaactgcc
    23041 tatacacaag atttctaacc ttagtcgaca tgagctgaaa cctcttattt gttaagtagt
    23101 tcacaaaata ttatacacct attttatgaa tagtcaactg tctttacagt aaaattttag
    23161 aaaatcatga aaattttctc tttctttcca ttttaagtga cattcagtca ttctcacatc
    23221 aaaaaagccc agacgaaatt gtctgagcat tcttttatct agtcgtttaa ggaagttgag
    23281 ttcagtatgt ttaaagtctc tgtcccatca tttcttcaac aaaccttgtt cttggagaaa
    23341 ctccttggct acttgctttg ctgacttgcc ttcaacaccg acttggtagt tgagctggct
    23401 catctggctt tctgtaatct taccagccaa tgtattaaga actctttcca actctgggtg
    23461 tttcttgaga agagcttctt tcatgagtgg agccccttga taaggtggga agagttgctt
    23521 gtcatcttcc aagacctgta aatcataacg ctccaattcc gcatcagtcg aataggcatc
    23581 cgtgatttga atatcccctg actgaattgc ctgataacga agagctggct gcatagtcgc
    23641 cacattgaga ttgagaccat acattgattg caagccctta tttccatctt cacggtcgtt
    23701 aaactcaagc gtaaagcctg ccttcagctg cccttccact tttttcaaat ccgaaatggt
    23761 cttcaagcca tattcttgag caatcttttt cggaacagct acagcatagg tattttgata
    23821 agacataggt ttgagatagg ctagatgatc ctgcttggca atgccatcac gcgccacttg
    23881 ataaacctgc tctggctcat gactcacctt gggtgatggt tgaagcaaac tttcagtcac
    23941 cgtaccggta aattcaggat aaatgtcaat atcacctttt ttcagagctt cataaaggaa
    24001 acttgtcgtc ccaaaattcg gtttaacagt cgcagtcatg ctggtatttt cttcaatcag
    24061 caatttatac atattagcca aaatttctgg ttctgggcct attttcccag caataatcaa
    24121 gttttccttc tctttttgag ccaaaagggc tggactataa gacagaccca gtaataaagc
    24181 caccaaggca aaaccagaga aaatcgtccg caatttcgct ttttccatta cttttagtag
    24241 gaagttaaag gcaatagcta gcactgcaga agaaagggcc ccaatcaaaa tcaaactggc
    24301 attattacgg tcaattccca acaggataaa ggaacccagt ccccctgccc caatcaaggt
    24361 cgccaaggta gccgtaccga taatcaagac cgctgccgtc cgaattccag acatgataac
    24421 aggcatggca agtggaattt caaacttctt gagacgctcc catctggtca tcccaaaggc
    24481 aatcccagcc tcttgaagac tcggatcaat tcccttcagc ccagtaatag tattttgtaa
    24541 aatcggaaaa atcgcataaa tcactagagc tgtcaaagcc ggcaaggtcc caattcccat
    24601 caaagggata aagagcccca acaaggccag agacgggatg gtctggaaaa tccctgcaat
    24661 ctgtaagacc caatccgcca gcttctcatg atagcgaaga aaaacagcca agggaatcgc
    24721 aagcaaaata gctagtaaca aggtcaaaag tgacaactgc aaatgttgag atagagctgt
    24781 caaccaatca ctaaaacgat cctgaaaagt tgcaattaaa ttagtcatga acactacctc
    24841 caaacaagtc tgctacaaag tctgttgcag gcacttttaa aattgtctcg ggattcgcta
    24901 cctggcgaat ttctccatcc tgcaagacag caatacggtc cgccaacttc aaggcttcat
    24961 ccgtatcatg ggttacaaaa atcgttgtca tcccaaactc tttatgcaat tcttttgtca
    25021 gaacctgcaa ctgttttctc gaaatagcat ccaaggccga aaagggttca tccatgagga
    25081 aaatcttggg ctgaccaatc atagctcgga caataccgac ccgttgctgt tctccaccag
    25141 ataattcact aggtaagcga tgcccatact cggctactgg taaaccaacc ttagccaaaa
    25201 gctcttctgt tttcttcgta atttcttcct tgctccaccc cttcatttca ggaatgagag
    25261 caatattttc cgcaactgtt agatttggaa aaagagcaat agcctgtaaa acataaccag
    25321 tagaaagacg aagttcacgc tcatcatagt ctttgatgcg cttcccatcc atataaatat
    25381 ttccatcagt tggttccaaa agacggttaa tcatcttgag catggtcgtc ttacctgacc
    25441 cagaaggacc tactaaaacc ataaattccc catcctcaat ctgtaagttg acatctctca
    25501 agacatcctt ttctgtgtag cgcagtgcta catttttgta ttcaatcatt ctttgtcctc
    25561 aatttaaaac ttccctcgat tagtcaagtc ttctacctta ggcatgactt ccttattatc
    25621 ccaatgctcc acaattttcc cgttctctaa acggaagata tcgtactggg cataagcaac
    25681 gccatcaatc tgagtctgac catagctaac cacatagttt ccttgtccta agagttggaa
    25741 aacaaagtca aaagtgacac tatattcagc cacatagttt ttataagcag cacttccttg
    25801 tccaatctca tgattatgct gaatcaaatc gtctgccaca taatcactcc actgctctag
    25861 ctccccattt tggaaaattt ctgtcaagaa acggcgaacc agctttttat tttctgcttt
    25921 cttatccaaa tccttgattt caaaatctcc aaaaatttga tctagttggt cattttcagg
    25981 tgttcgatag tagtcaatga catcccaatg ctcaacaata caaccattct catcctcacg
    26041 gaaagtatcc gtcgtcaccc attgagcttc tccaccattc agatattgat gaacatgaac
    26101 aaagaccaga ttgccatcct caatggtgcg gacaatctta atctgacgct ctggatgacg
    26161 ctcaaagaaa tctgcaaaga aggctgcaaa tccttctttc ccgtcaggaa cacctgtcga
    26221 atgttggata taggtatccc ctacagactg ggcttgagcc tcagcaactc gcccgtcttg
    26281 aatggcatgg atgtataggt tgtgagcatt tttcacttgt tgtgacatat tctaaacctc
    26341 atttcccttc tctttcagat tcgccaaaat tctttcttga aaaccttcaa attggtgaat
    26401 ttcttcctct gaaaatcctt tgtaaaagat agtatccaat ttctgactga cacgatgccc
    26461 cacttctttc tggaacttgc ctaactccgt taaaactaaa tacttcttac gcttgtcttt
    26521 tccacacgga ctaacaatta caagcttttg ttcctctagc ttttttatca tagtcgtcag
    26581 cgtattattc gcaagcccag tcgcaagcgc gatatctgtc gcagttgcgc agccagtttc
    26641 actattccat aaaaccgcta aaatcttgcc ctgttcaccc ctataaagag cctcaggatc
    26701 ttgactcagt aacttttgaa aaatccgccc attcaacaaa cgaatatgat gggctagcaa
    26761 atgactatct ttcataacac ctccaattta tttcgatatc gaaatgaata aaataattgt
    26821 aacactcatc gttctaactg tcaactattt cgatttggaa ataatttttg ataattatcc
    26881 acaccaccat actccggctc aactaacttt taacgagagt ttctaaactc cttcgtcctc
    26941 cagtctacaa aagccttcca ttcgtactat cctatatttt atgaggggac acatttttcc
    27001 tatcagacca tttattttaa agatagaagt aaatcataat tgcttccatc tgttctttta
    27061 tagtatattg aagttagact agagcactgt atcttctaaa acattgatag aaagcgattt
    27121 gaatttccca atcaatttgt tcgtatttat agcattttga aactagaata ggacaccatg
    27181 actgctaaaa gatttctata aattcatttg attttcctaa tcaatttgtt catatcttat
    27241 ttcattccgc tataatttca ccttacccta tctttttcgt agcacccttc aaacagccta
    27301 tcccctaccg tttgacgatt cctcacttcg ctccacttcc attacagaag tttcttcact
    27361 actatgggct cggctgactt ctcatgattc cttgttacta ctatttgaac gctcacgaga
    27421 tagatcttac aaaaaatgct ttgatccaca atggaatcaa agcattttaa agagttcctc
    27481 atacataagc gcagaagtcg cagttcctct gtacttggct tcttctcttt tgataaagcg
    27541 agccaagttg agcaactcag gtgctggatg tttgggattt aggagcaatt cacgattgac
    27601 caggcctgag agacgaactg cctgcaattg ctcatttgta gtaggcagtt ttttagcagt
    27661 ctctaggaga gcagcaacta aatcttcact caaatcatgt cgagcatgat tgtaaagatc
    27721 ttttataagg ctttctaggt ttggttctgc catccctacc acctccctta tggtttaata
    27781 atgtttaatc aaatcaaccg ttgaacgatc caatttcttc accaaggctt gtaagaaagc
    27841 ttgcgcttct aggaagtcat ccattgcata gagggtttgg tgagaatgga tataacgagc
    27901 gcagacaccg atagttgttg atgggacacc accatttttc agatgagctg cacctgcatc
    27961 tgttccgcct ttaccacagt agtattggta cttgatacca gcttcttcag ccgttgtcaa
    28021 aagaaaatcc ttcatccctg ggagaagcaa gtgacctgga tcatagaaac gaatcaaggt
    28081 tccatctcca atcttgcctt gaccaccgta gacatcacct gctggtgagc aatcaactgc
    28141 gaggaagact tctgggtcaa acttggttgt agaggtatga gcgccacgca gaccaacttc
    28201 ttcttggacg ttagaaccca gatagagttc attgccgagt ttttgacctg ataaagcttc
    28261 agctagctcg cttaccatga ggacaccgta gcggttatcc caagcttttg agatgatatt
    28321 tttttcattg gctgtcaaaa ttgcagaact atctggtacg atggtatcac caggacggat
    28381 gccaaaactt tctgcctcag ccttgtccgc aaaaccacca tcaaaaacga tatcggcaat
    28441 ggctggcatg gttggtcccc cctttccacg agtcaaatgc ggaggaacag aacctgaaat
    28501 cacaggaatt tcatgaccat cacgagtcaa gagtttgaaa cgttggctgc taaccaccat
    28561 ggggttccag ccaccgattt ctacgacacg gaaggtacca tctggcttga tttcgctgac
    28621 cataaaacca acttcgtcca tatgagaagc gaccaagacg cgcggtgcat ccacagcttc
    28681 tgaatgtttg ataccaaaaa taccacccaa gccatctgtc accacttcat ccacatgcgg
    28741 tgtcaacttt tcacgaagat aagcacggac aggcgcttca tgacctgaga ctgcagcaag
    28801 ttctgttact tctttaattt ttgaaaataa tgttgtcatt tcagttcctt ctttctttca
    28861 tccattttac cactttttat aggagaagga tagtgggaag gtggatttct aagttagtat
    28921 cttagtcctg ctctatctta gaaaaggata gtattctctt gcatgtagtg caaaatctag
    28981 taaacattcc aaaattaact cgaatattta tttccaaaca aaaaaacaat acaccatcaa
    29041 agttgtttgt atttttcatg aaatttacag aaaatagttg acttcccttt cttctttctt
    29101 taaatatata gttggttgag tttggaatag tacgctgtag ctgctgaaac atttctagaa
    29161 attaatttga ctttcctaat agagttgttc atatcttatt tcaatttact atagtacaaa
    29221 actagaaaag gaaaaaatca tgaccaaaac aattcttgtt acaggtggga ctagctacat
    29281 tggctcccat accgttaaag ctcttttaaa tgctggctat aaggtgcatg tcctcgataa
    29341 tctctccaca ggaaatcgag cggctgtgga tagtcgtgct agctttaaag aactggatgt
    29401 ttatgatgct agcgccttaa aggcttactt agaagaaaat cagattgatg ctgtcctcca
    29461 ttgtgcaggt gaaattgttg tgagcgaaag tattgaaaat ccaagtaaat acttcactgc
    29521 caacgttgct ggtatgaacc aagttctcaa agtcttatct gaagttggca ttcaaaaaat
    29581 catgttctct tcgactgctt ctctctatgg aaataactgt attgacaagc cagtgactga
    29641 agataccctg ctcgaccctg tcaaccctta tgcagagaca aaactgatgg gcgaacgaat
    29701 gatttactgg atggccaatc gctacgactg gaaatatgtt attttccgtt actttaatgt
    29761 tgctggggct gaaatggatg cttcaaacgg tctgcgtgtg aaaaatccaa ctcacatcat
    29821 tccaaatatc aacaagaccg cattgggaca aaatgatagc ttaaaaatat ttggagatga
    29881 ctacgataca cgtgacggtt catgtattcg agattacatt tatgtcttgg accttgcata
    29941 ggctcatgtt aaaggaatga actacctatt tcaagaagac agttcttctc aaatctttaa
    30001 cttaggaact aaaaaaggct atactatcaa agaaatcttt aaaactgctg aagaattact
    30061 aaattaaaaa atcccacacg agattgttgc tcgacgtgct agtgacccag ccagtgtcct
    30121 agcagatgct tcaaaagcta aacaatatct tgattggacg gctagctact ccctcgaaga
    30181 tattatttta tcagattaag gtaaaaacat tttagaatag gataacaaag gagaaagtct
    30241 tgttgctctc tccttgttgt atttattaaa ttgtcagaaa ttttattgac agattagaaa
    30301 aataatgtta catttatatc cgcagatatc tttcgatacc aaatctacat gaaaggacgg
    30361 ggtatgaaac tttctcatta tttaattggc ttacttctac tcctagtctt tctctctatt
    30421 agcattggga ccagtgattt ttcatgggga aagctatttg atttcgacca gcagacatgg
    30481 ctcctctttc aagagtcccg tctcccaaga actatcagta ttctcctgac tgcctctagt
    30541 atgagtatgg caggccttct catgcagact attacccaaa atcagtttgc tgcaccgagt
    30601 acagttggaa cgactgaagc cgccaaactg ggaatggtgc tgagcctttt tgtctttcca
    30661 tcggctagtc tgacccaaaa gatgctcttc gcttttgttt catccatcgt attcaccctc
    30721 ttcttcctag cctttatgac catttttact gtaaaggaaa ggtggatgtt gcctctgatt
    30781 gggatcatct atagcgggat tatcggctca gtcacagaag ttatcgccta tcgtttcaat
    30841 ctggttcaga gtatgactgc ctggacccag ggctccttct ccatgattca gacccatcag
    30901 tatgagtggc tcttcttagg cctcatcatc ctgataaccg tttggaaatt atcccaaacc
    30961 ttcaccatca tgaatctggg gaaagaaatc agcgagagtt tggggatttc ctactcccta
    31021 cttgaaaaac tggccctctt tctggtggcg ctaacgacaa gcgtcaccat gattaccgtg
    31081 ggtggcctac catttctcgg agttatcgtt cccaatcttg ttcgcaagcg ctatggagat
    31141 aatctaagtc aaaccaaact catggtcgca ctggttggtg ccaatctagt tctggcttgc
    31201 gatatcctat cccgagttct gattaggccc tatgagttgt ctgtcagtct cttgctagga
    31261 atcatcggta gtctcggctt tatcctactt ctctggagag ggggacgaaa agatgcagac
    31321 taaaagcaaa cataccaagc tcttctggat tctcattatt cttgccatcg gagcttgtct
    31381 tctctacttt tggcccatca ctcacttgtc agcctttgct tggaagttgc gttcccaaaa
    31441 gatcatcgtt tatctcttgg tagccatcgc gactgggatt tcgaccatta gttttcaaac
    31501 cctgacggaa aatcgcttcc tgacgcctag tattttggga attgaatcct tctacgtcct
    31561 actacaaacc ctactactgg tttttgaaag caagtttctt caacttggca aatcccctat
    31621 cttagaattc ctagtcttac ttcttgtcca gtccctcttc tttctcacct tacaaggtta
    31681 cttgaagaca ctgatgaagc aagacctggt cttcatcctg ctgatctgtc tagcgctcag
    31741 aagtctcttt cgaaatatca gcaccttcct tcaagtccta atggatccaa acgaatacga
    31801 taaactgcaa aatagtcttt ttgcctcctt tcaacatctc aacacttcca tcctagccat
    31861 cggttctctg atcatcctcg ctttgacaat ctttttcttt cgaaaagcag tcgttctaga
    31921 tgtcttgcac ctgcaaagag aaacggctca gatattggga ctcgatgttg aaaaagaaca
    31981 gaaagagctc ctctggggaa tcgtgctttt gacctcaacg gccactgcct tggtaggacc
    32041 tatggccttc ttcggcttta tgctggccaa cctcacctac ctgattgtca aagactatca
    32101 gcacaagtta ctctttatag tggccattct ggttggattt attagcttaa ccttggggca
    32161 agccttgatt gaacgagtct ttgcactgga aattcgtatc agtatgatca ttgagagtgt
    32221 gggtggcttc ttattcttta tcttactata taggaggtct cgtcagtgaa actggaaaac
    32281 attgacaaat ccattcaaaa acaggatatt ttgcaaggca tttcgcttga agtcagtcct
    32341 caaaaactga cagcctttat tggtccaaat ggtgctggaa aatcgactct cctctccatc
    32401 atgagcagac taaccaagaa agatcaggga gttctcagta tcaaaggacg tgaaatcgag
    32461 agctggaatt cgcaagaact ggcccaagaa ctaaccatcc taaaacagaa aatcaattac
    32521 caagccaaat tgactgttga agaactggtc agttttggac gttttcccta cagccgaggt
    32581 cgacttagat cagaagactg ggaaaaaatc cgagaaactc tgaactattt ggaactgacc
    32641 aacttaaaag accgctacat caatagcctg tcaggggggc aactccagcg cgtctttatc
    32701 gctatggtac tggcccagga tacggacttt atcttgctgg acgaaccact caacaatctc
    32761 gatatcaagc aaagcgtcag catgatgcag attcttcgac gactggtgga ggaactcggc
    32821 aagaccatta tcatcgtcct ccacgatatc aacatggcca gtcagtatgc agatgaaatt
    32881 gtcgccttca aggacggcca ggtctttagc aagggaagca ccgatcaaat catgcaggct
    32941 gacctgctca gtcaacttta tgagattccc atcacgctag ctgatatcaa tgacaaaaag
    33001 atctgtatct atagctagta acataaaagc tcaagttaga gaaccttcag tctcttagtc
    33061 aataagatta agagactccc taaatcgtta tcacatttta aaaaggagaa attatgaaaa
    33121 catcccttaa actttatttc actgccctag tggccagctt cttgctccta cttggtgcat
    33181 gtagtacaaa ctcaagcact agtcagacag agaccagtag ctctgctcca acagaggtaa
    33241 ccattaaaag ttcactggac gaggtcaaac tttccaaagt tcctgaaaag attgtgacct
    33301 ttgacctcgg cgctgcggat actattcgcg ctttaggatt tgaaaaaaat atcgtcggaa
    33361 tgcctacaaa aactgttccg acttatctaa aagacctagt gggaactgtc aaaaatgttg
    33421 gttctatgaa agaacctgat ttagaagcta tcgccgccct tgagcctgat ttgattatcg
    33481 cttcaccacg tacacaaaaa ttcgtagaca aattcaaaga aatcgcccca accgttctct
    33541 tccaagcaag caaggacgac tactggactt ctaccaaggc taatatcgaa tccttagcaa
    33601 gtgccttcgg cgaaactggt acacagaaag ccaaggaaga attggccaag ctagacaaga
    33661 gcatccaaga agtcgctact aaaaatgaaa gctctgacaa aaaagccctt gcgatcctcc
    33721 ttaatgaagg aaaaatggca gcctttggtg ccaaatctcg tttctctttc ttgtaccaaa
    33781 ccttgaaatt caaaccaact gatacaaaat ttgaagactc acgccacgga caagaagtca
    33841 gctttgaaag tgtcaaagaa atcaaccctg ccatcctctt tgtcatcaac cgtacccttg
    33901 ccatcggtgg ggacaactct agcaacgacg gtgtcctaga aaatgccctt atcgctgaaa
    33961 cacctgctgc taaaaatggt aagattatcc aactaacacc agacctctgg tatctaagcg
    34021 gaggcggact tgaatcaaca aaactcatga ttgaagacat acaaaaagct ttgaaataag
    34081 ccacttcaaa ctcaatttaa tactcaatga aaatcaaaga gcaaactagg aaactagccg
    34141 caggttgctc aaaacactgt tttgaggttg cagatgaaag ctgacgcggt ttgaagggat
    34201 tttcgaagag tataaaattt tgacattcta aaacgcatcc tatcaagttc actcaaacct
    34261 gataggatgc gtttttatca ttttgaaatc tatttacccc acctcattcc ccttcttgat
    34321 ttcgtttaag ggaaaagtgg tctggaacgg ggtccttacc tgttttcgac cagggatgac
    34381 aacgtaaaat ccgagccaag cccatcaata cccccttaaa accatgtttt tcaatagcct
    34441 gaatcatgta gttggaacaa gtcagctcaa agcgacaaga gggtggaaag actggtgaga
    34501 taaaacgttg gtaaaagcgc acaggcgcta ttaaaattcg tttcattatt tcttagttac
    34561 agccatggtg tgtagttggc tgatttcttt tttattaaga cgacgggatt ctcctggacg
    34621 gagtcctgtc aagtctaggt gtccgaaacg agtccgagac aacttatcta cttggagacc
    34681 aacagcttca aacatctttt taacctgatg gttacgccct tcatggatgg tcaactgcac
    34741 cacagagcga tttttgactg ggtccacttt gagaatttca taaacagctg gcttggtttt
    34801 cttaccatca atctcaagac cacgggtcaa ggggcggaga ttgtccttat tggccacacc
    34861 cttaacacgc gcgacataaa ccttgtcaat ctcattacga gggtgaatca tctcgtctgt
    34921 aaagtcccca tcattggtca aaatcaagac acctgatgta tcccagtcca aacgacccac
    34981 agggtaaata cgctctttga cattgggcaa gaggtcgaca accgtcttgc gacccttatc
    35041 atctgtcaca ctggaaatca caccgcgtgg tttgttaagc agatagtaga ccttttcttc
    35101 gttgtagata ggttgacctt caacttcgac cttgtcgcct gacttgatag tggttgctag
    35161 ttcacgcacc acttggccgt taaccgtcac caagccttgc ttaatcagct cttctgcttt
    35221 tctcctactg gccacacctg cgtgggcaat atacttattg attctcatct tcttctatcc
    35281 tttcaccaaa taattggctt tcttgggctt gaatctcaag ctcatcaatc actggtaatt
    35341 cttctaaatg gtttatcccc atgtaatcta ggaaataatc cgtagtcaca tagaggttgg
    35401 ggcgccccaa tacttccttt ttcccgtctt cctttatcag gtcaaaagcc tgcaactttg
    35461 ccaaggctcc actcgagtta actccacgga tggcatcaat ttctatccgc gtaatcggct
    35521 gtttgtaggc aataatggac aaggtctcaa gggcagcccg agacaagctc tggttgatag
    35581 gcgccttaga gtattccttc aaaatctctg caaattgaga cttggtcacc aatctataag
    35641 caccacttgt ctcaatcaaa gccaaactgg aatctgggtc cttttcatac ttctgggcta
    35701 attttcctaa actctgctgg atgcctgtcg gtggcagaga gaggagttca gctaactggc
    35761 ggacccgaat cccatcttca cccgctacaa acaagagcgc ttctattttt gctaaagtac
    35821 tcatctttcc tctctatcaa gtctagcttt gaggcacttg actttcttcc ttcttttcca
    35881 tgagatagat atctccaaaa ctctcctctt gcacgaggat cagctcctgg gttttgatta
    35941 actctagggt tgccaaaaag agggtgatga cctcttggac attctgggct tccttgaaca
    36001 aatcctgcaa gcgcaattga tctcgtccaa tcaaggactc tttcacgata atcatcatgt
    36061 cctcaatctt atactcatcc cgcaagatag tcgtgtgatt ttgtgcaaac tcctcttttt
    36121 tcttggctag gatatttgaa aaagccaaaa agaggtcaat ggtcgtcttg tcatgcacaa
    36181 gctccgcatc ttcgtaaatc aactctgtcg gcgctttgga ataatactgg gcccgttctt
    36241 ggtgcttggc ttccaagtgc tcacccaaga gcttgaactt gcgatattct tcgatttgag
    36301 agaggaggtc ctgctccagg tcatccccca agtctgtcac ttctgctacc ttcggaagga
    36361 gtttacgact cttaatcagc atgagctgac tagccatgac catgtactca cccgtcactt
    36421 ccagacgcat ggcctgcagg gttgaaacat aggctagata ctgttcgatg acttccgtaa
    36481 tgggcacatc gtagatatcc atctggtact tagaaaccag atgcaagagc aagtccaggg
    36541 ggccttcaaa atcttttaat ttaatatcca ttatctatat ttttctaagg tcaggactgt
    36601 ttttaatcct aatttttttg caatttcgta caaatcgacc ttgttttcta tttgtcttag
    36661 aataaactgt tcacgtaaga cttgagctga taaggatgga aaacctttct ccttgacaaa
    36721 agattctaac tgacgaaagg cccactgacg agaatagggt ttctctcctc tttcaaaaag
    36781 ataggtctgc cccatcaagg gttccaattc tgaaagcaag gccgtgggaa tggtgacaat
    36841 cctctgttgg gaagccttgc tgattcgcaa cacctgaaaa tccagattga tgtccgcaac
    36901 cttgatggct aaaatctcac tgggcaagag ccccatttct aggattaaga gcgctagcaa
    36961 gcggccctct ggatggtcgc tttcctgcca aaaagagtct aggtatagaa tctctggctt
    37021 ttccgtcttc ttttcagctt gtttggctaa ttccaagcgg taaaagctgt ccacctctcc
    37081 tttttgatag agaaagtata gaaattggtt acaggccgaa atctttcgct tctgggcgct
    37141 gatttttaga ttggctagct gggcttggta aatcttgaga ctggtctcag aaatccgctc
    37201 acctaccatg tctaaaaatt gctccaaatc atacttatag gactgcttgg aattgacaga
    37261 caagccctgc ttttcctcta aaaaggctga aatcctgtct ctcattggca tcgaatctca
    37321 tattccttac taaagtcatg caagagggca ttaacggcct tgaggatgga cttacgcgta
    37381 ataatccctt ggaaaatacc ctctgcatcc acaaccggta agaaggactc atctactagc
    37441 ttgtgcaaga cctccgtaat ggtgaaatca ggcgaaacaa ccgctacgtc cgtttttgtc
    37501 atatgaacga tatccgtatc cgccatgatt tcttggctca agtcatgctc catctgataa
    37561 gccataatat ctctgagtcc aatcgtccca acaaactgtt tttcatctgt cacaacggga
    37621 acacgggtat aggtcatctg actgagcaag agggtcgcat gatccgcatt gtgggtatca
    37681 atcaacacag ctagattttt agcaggggtc aaaaaagttt cctcctgccc caacaagaaa
    37741 gtctcaaact ccttggcaat catcggctaa actccttgga caaacctgga tacacctcgt
    37801 gatctcgtgt caaaaagtcc actttgaagt aactatcatc aatctccaca cgagcataga
    37861 gacattctct gatggtacct cgtggttgac tgatagaacc tggatttaga aagaggatct
    37921 tgccttccaa ccaagcactt ggcacatgca agtgaccata gaggcagata gcggcctctt
    37981 cctcctgagc ccagtagtcc aacttttgaa agttgaaatt gatgtcaaac aagtgaccat
    38041 gagtttggat aatcttggtc gaaccaagct cagtcaccag acgttctggg tagccggcgt
    38101 agaagtccat gtttccttta acaacgcgga tgccctccca aagtggagaa tccggacgta
    38161 gttcagaatc gccgttatga aaaacggcat cgactttgcc cacatagcga tcacggactt
    38221 cttccacaat caagctatcg ccatgggaat cgctcattac aatgatggtt tgctttgcca
    38281 tgatggaaat acctccaaaa gtttcttaac ggctaaggca cggtgagatt gactattttt
    38341 ttcttccagg gttaattcag ctgatgactt acctgtctct cctacaagga agaggggatc
    38401 atagccaaag ccattttcac ccttaggttc aaagttaata taacctgacc agtctgcttc
    38461 aacaactaaa ctttccttat ttgggctggc tacgactagg gttgtgtgga actgagccga
    38521 gcggtccttg agttcaaaga ccatggccaa ttcgtgcaag agtttggcat tattttcacg
    38581 gtcagttgct cccacacctg cgaaacgagc tgaccagacg cctggtaagc caccaaggac
    38641 atcgactttg agaccagaat catctgccaa aaccatcttg cccgttaatt gagaaatggt
    38701 ttctgcctta aggcgggcat tttcttcaaa ggtcatacct gtttctgcta cttcaggcag
    38761 gtcagggtag tcattaagat tttccacatc gtagcctaac ttatcaaaga tagctcggaa
    38821 ttccttggtt ttgccttcgt tacgagtcgc aatcaacaag gtttctctga ccttgcttgt
    38881 ctcaaagaaa tcatgaacat tgaccccttc tttaggcaat tctacataca agagttgccc
    38941 attttcaacc aagagcaggg ccccatgacg ttgaataact tccaagtttc gtcccatttc
    39001 ttgattaagc atgtctacca caaaggagaa caaacggtag gtagaaccat agcgtaaaac
    39061 agtaactgaa agcgggaaac catactcttc atctccaaat attttggaga attcaaacag
    39121 aggaaaatct gtcttatagt cgctcaaact gttaacgcca ccaaaaatac tataagaccc
    39181 aacataccaa tcctggtcat ccttatattc ataaatttta tttgtcataa ttctacatgc
    39241 tccacataaa tctctttttc cagccattct tcaccaattt gtgcaaaact ttggctactg
    39301 gctgttgtgt aaaaacggtg atggagtggt ccagcatcgc gaccacgatt gatttcaaaa
    39361 taattgagta agactgagat atcccgtacg cactctgccc cactatcgat gagctgaacc
    39421 tttggcccca tgacattttg gataataggg cgaaggagtg gataatgagt acagcccaaa
    39481 atcaggctat ccacctttcc aaccaaggga cgcagggttt catagaccac cttcttggta
    39541 acactggttg acagggcacc tgactcaacc aagggagcaa acttgggaca ggccaagctc
    39601 tccacctgta agtcgggatc cagatcatgg attttctgac ggtatatgtc tgattgtacc
    39661 gtcatgggcg ttccaatcac tccgattttc ccaccttgac tggacttgat ggctgccgaa
    39721 gctcctggca aaattacacc caagacagga atatctagtt gagccttgat ttcttcccag
    39781 acgaccgcag tcgcagtgtt acaagcaatg acaatcattt tgacatcctt ggtcaagaga
    39841 aagttgacca gctgccaagt atattcacga atttgctcag ctggacgggg gccatagggc
    39901 gcccgcgccg aatctccaat atagacgatt tcttcatggg gaagctggcg catgagctcg
    39961 cgcacaacgg tcaagccccc gacacccgaa tccaaaaaac caattggtcg attatccata
    40021 cagtcttctt tctagtcttt tttctgattg atcgttcatt atgattcctt gtactgaacg
    40081 aactgaccga cagcttgata gaatgtcaac tacctctctc ttaatcataa tcaaatatca
    40141 atagaatgca aggaaaaagt ctcatcccta aaccataacc aacatagtga gaagtctgga
    40201 actttcatcc cagacttttc cttatttatt ttttcttttt attgttagca agggctgcct
    40261 tttgttggcg gatgatttgg tggtaaactt gttgtacctt agcttcgctt ggtttttgac
    40321 catttgcact caaaagagta cgaactgctt cagcattcaa acgtgggttg tcagcaaatt
    40381 ctttttcgat ttgcttacga accaagtaca ttcctccaag agctcctcct agaaaagcta
    40441 gcacaatcaa tacaattgct aaaagtaaat ccatcatttt tctcctgttt ctttttgagt
    40501 ccctttcatt ataccacaaa ctagccaccc tgaccagttt gttctacact ttcagggatg
    40561 gaatagacag caaaaagaac cctgcttttc tgaaaaagag gtctccttgc taagtttaat
    40621 ttacataaat agccagtgtt tgatagggtt ttagtagaat tttctcagac acttctgcat
    40681 cttcatagtt tgaaatcaaa atctgtccat tttggtagac tgctggcaag tcgatttcca
    40741 cttctttagc ataaaagtta ttgagcacta gtaacttttg atcctcaaac tggcgttcaa
    40801 aagcgtagac ttgtttgcta tcttcaaagg ctggtttgta acttccttct gaaatgattg
    40861 gcatttcctt acgcagtcga atcaagtctt gatagaaggt aaaaatcgga ccctggattt
    40921 cattttctac attgatgtct ttataggatt taccagcttt caaccaagga gtgcctgttg
    40981 aaaatcctgc attttccgaa gcatcccact gcatgggaat gcgtgaatta tcacgcgact
    41041 tagcttgaat aatctggaag gcttcttgct gactctttcc ttcttctagg agcatctgat
    41101 aggcattaag cgattcgaca tccacgtaat cagccataga atcatagtct gggtcaatca
    41161 tcccgatttc ctcacccatg tagatataag gtgtcccacg tgacaggtga atgctggctg
    41221 ctagcatggt ggctccttcc ttgcggaagt tttgaatatc gacaaaacgg ttcaaggcac
    41281 gtggttgatc gtgattgttc caaaagaggg cactccaacc gtctttatca ctcatttcct
    41341 taccccaact atggtaaaga ctcttcaact cttcaaaatc aaagggagcc aaggtccact
    41401 tttgtccatc cttatagtcc accttgaggt gatgaaaatt aaaggtcatg gataattcct
    41461 gacgatcagg cgacgaatag aggacacagt tttccatggt ggtagaagac atttccccaa
    41521 ctgtcataaa gctatcgtca gatccaaaag tggcttggtt catcatacgc aaatagttat
    41581 gaacgatggg tttgtctgta taagctggct tcccttcatt ttcaggacag tccactgaaa
    41641 cctcgtcctt accaatcaaa ttgatcacat caaatcgaaa acctttgaca cccttgtcgc
    41701 gccagaaatt aacaaccttg aaaagctcct tacggacatt gggattgcgc cagttaaggt
    41761 cagcctgggt ctcatcaaat aggtgaagat agtatttccc agtatccccg aaaggcgccc
    41821 atgcagaacc accaaactta gactgccaat ctgttggttg gtcttggatg aagaaaaagt
    41881 cttgataata cttatcacca gctagggctt tctgaaacca ttcatgctct gtcgaacaat
    41941 gattaagtac catgtccagc ataaagtcaa tcttgtgctc tttaccgata cacaccattt
    42001 cctcaaaatc agccatatca ccaaaaagag gatccactgc catataatct gaaatatcgt
    42061 aaccattatc ccgttgagga cttggataga atggattgag ccagaccata tccacaccta
    42121 gtttggctag atagggaatt tttccgataa tcccacggaa atccccaata ccattgccag
    42181 tggtgtcttt gtaagatttt ggatagattt gatagactac ttttccttta tcaagtgtca
    42241 tctgtttctc cttttctgat aaaagggagg aagcagtctt ccgtccctat ttgtgctatt
    42301 tcaattatac tcaatgaaaa tcaaagagca aactaggaag ctagccacag gttgctcaaa
    42361 acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc gaagagtatt
    42421 agattcgtgt agcgaccatg agagatgctc cagcttgaat cgttgtcgga taagttccgg
    42481 gaatagtcgc tgtataagca tcttggttgg tgatgataac aggagtttct gtcaccagac
    42541 ctgcagcctt aatgacatcc atatcaaaac gaatcagttg ctgaccaact gtaacgtgat
    42601 ctccttggac tacaagactt tcaaaacctt tgccatcaag acctactgta tccataccga
    42661 tgtggatgag caattcaact ccctcgtcag agacaatgcc gatggcatgc ttggtaggga
    42721 aaagaaccgt cactgtccca ttaactggag aggtcaactc accttggctt ggttcaatga
    42781 ctagaccttg ccccatgaca cctgatgcaa aaataggatc cgtcgcttga ctcaattctt
    42841 tcacttggcc agttagtggg ctgataattt ctaccgaagt aagttctact ggttcatggt
    42901 tcacaaattc tgcttcttct tgagcaacga attctgcctg caagttcgta tcgccctctg
    42961 tttttgtaaa gagaccagcc ttgcggaaga agaaagtcaa gagcattgga acaacaatcg
    43021 caactagcat agttcctgca aatggcagca tgtattgagg ttgaatagag agaatacctg
    43081 gcaaaccacc gataccaata gaagccgcag ttacattaaa agtaacggat aacatgcctg
    43141 caagggctga accagtcatc ccagcaacaa atggataaat atattttacg ttaaccccaa
    43201 aaagagctgg ttctgtaaca ccgagatagg ctgaaatggt tgcaggaagt gaaacctgag
    43261 cctcacgctc atcatggcga tgcatgaaat aataggcaaa cacggctgag ccttgagcaa
    43321 tattagaaag agcaatcatt ggccataggg cagtgccacc agcatccgca atcaattgtg
    43381 tatcaatggc attggtcata tggtgcagac ctgtgatgac aaatggagcg tagagggcac
    43441 caaaaattgc accgaagagc catttaactg gaccagttaa acctgccaag acaactgatg
    43501 aaagtccttg tccaattgtc caaccgattg gtcccaaaac agtatgagcc aaaatcaagg
    43561 ctggaatcaa tgacaagaaa ggtacaaaaa tcatagaaat gacttctggg atatgcttgc
    43621 gccagaagat ttcaagataa gatagactca aacctgcaag caaggctggg ataacttggg
    43681 cttggtaacc gatacgatta acagtaaaat agccaaaatt ccaaacccag tttgccgcga
    43741 tatcagctgc tggcgttgaa gcaaccgcat aggcattgag caactgaggc gataccaaac
    43801 agattccgag aacaattccc aaaatttggc tggttcccat cttacgagaa acagaccaag
    43861 taatccctac tggtaagaac tggaagatag cttcaccagg caaccagagg aaatgattaa
    43921 cacctgccca aaactgagag gattctgtga tggtcttgcc atccaacatc gaccaatgga
    43981 caccttccaa gacattacgg aaaccgagga tcaatcctcc gactatcaag gctggaataa
    44041 tcggagtaaa aatctccgcc agagtggtca tgacacgttg gaccacgttt tgattactct
    44101 tagctgcaga cttggctgct tctttggaaa caccctcaat acctgaaaca gctgtaaaat
    44161 cattataaaa gatgggcacg tcatttccaa tgattacctg aaattgacct gcatttgtaa
    44221 aggttccttt aacagctgga attgactcga tagctttaac attagccttc ttatcatttc
    44281 ctaaaacaaa gcgcatccgt gttgcacagt gagttacggc agtcacattt tctttgcctc
    44341 cgattgcctg aagcagatct ttggcttctt gttcaaattt tcccatttga ttctccttat
    44401 acttgtcaaa gcaagtttga tttgataagt atattgtaaa cgataccaaa cttgtctgca
    44461 acttgtttgg tataaaaagg gtgttttttt gtaaattttc tagcacttgt atggtaaaat
    44521 agatacaggt gttcattaaa ctagactaaa aacctattta agcaggcaaa atgaagaaat
    44581 accaacaatt atttaagcaa atccaagaaa ccattcaaaa cgagacttac gctgtcggag
    44641 atttccttcc tagcgagcac gaccttatgg agcaatatca agtgagtcgt gataccgtcc
    44701 gaaaggccct gtctctcctc caagaggaag gattgatcaa aaagataaga gggcaaggtt
    44761 ctcaagtcgt caaagaagaa accgtcaatt tccctgtatc caacctaacc agctaccaag
    44821 aactagttaa agaacttgga ctgcgctcta aaaccaacgt ggtcagtctg gacaagatta
    44881 ttattgataa aaaatcctca ctgataaccg gtttcccaga gtttcggatg gtttggaagg
    44941 tggtccgcca gcgtgtggtg gatgatctgg tatccgttct ggatacggac tatctggata
    45001 tggaactcat cccaaatctc actcgccaaa ttgctgagca gtctatctat tcttatatag
    45061 aaaatggcct caaactcctt attgattatg ctcagaagga aatcaccatt gaccactcaa
    45121 gcgaccgaga caagattctc atggacattg gcaaagaccc ttatgtcgtt tcgattaaat
    45181 caaaagtcta tctccaagac gggcgccaat ttcagtttac cgaaagtcgc cataagttag
    45241 aaaaatttag atttgtagat tttgcaaaac gcaagaaata aaagactgag acaccagatc
    45301 tcagcctttt tctttatccc aaaactaatt catcactgac cagactttgg ccactatttt
    45361 tctggaagag atgcataagg tcttcaactg tcagatgctt tttctcttct cccttgacat
    45421 ccaccactat cttgccttga tagagcataa tgagacgatt gccgtactca atcgcatgat
    45481 tcatatcatg ggtaatcatc aaagtcgtcc actgatggta ctccacaatc ttttgcgtca
    45541 agtccatcac catttgactg gttttcgggt caagtgccgc agtatgttca tccaaaagca
    45601 aaagtttgag tttcaccaga gctgccatga ctagggtcaa agcctgtctt tgtcctcctg
    45661 agagatattg agtatctact tttaagcggt tttcaagacc aatattcaac tccttcaagg
    45721 cctcttggaa ctggattcta tccttctcct tcacgcccca accaagccct ctcttttgcc
    45781 cacgtctcaa ggcaatagcc atattctcct caatcgtcaa acgagaagct gttcccatct
    45841 taggatcttg aaaaacacgg gcaatatcct tggctctctt tctgacactc agatgtttaa
    45901 tggatttccc tgccagtaga aggtcccctt cgtccaccga tagattacca gccaagatat
    45961 tcatcaaagt ggattttcct gctccatttc caccaatcac agagataaaa tctccctctt
    46021 caacctctaa atctaatcct ttgaggacat ggttctcatt gaccgtccct gcttcaaatg
    46081 tcttgtgaat attttcaagt gttaacaaac ttgccataac tttctcctcc tattcatttc
    46141 tcaatttcag accacgaatc tttaatctct tttgcaatgc tggtgcaaat aaaaccaaag
    46201 cgagcaagat tgcattaaag agacgtacca agttttgatc tagatttgga atttcataga
    46261 tatttaaaat aatcaaacgg taaacaatag caccgattcc gatggataat aagcggcctc
    46321 caatggtcaa gtcgtgtatc aagacttccg agctcaaacc aacaacaatg gttcctgtcc
    46381 cagaagtcac atctgaaaat ccatcatttt gggcaaacaa ggaaccacat agggcaatca
    46441 aaccggttga aatcatgtaa ccaacaatct tcatggtgtt tacattgact ccattggcct
    46501 cactcatcgg gatattgtcc ccagtcgaac gcaagaccaa accaatctct gttttcatca
    46561 aaagggtcaa aaccaaacaa gtaagagaca gactaaacta agtgagaaaa cagctccttc
    46621 atttgtcaga cccaagctcg ccaactgttt aaagacggtt gcagaatctc ccaaggaaag
    46681 attgggtaca ctgcccatga ttttaatatt gattgaatag agccctgtca aggtcacaat
    46741 ccctgtcaag agagctggga ttttcatctt ggtgtggagc attcctgata taagacctgc
    46801 taccatacct gccagcaaag caagtaaagt cgcaagccaa ggatttgccc ctgcctgtat
    46861 ctgtgataca acgattgctg cccccatagg aaaggctccc tcagcagtca tatccgcaat
    46921 atccaaaata cggaaagtca agtagacccc aattgccata atcgaccaca acaaaccttc
    46981 tgataaacta gataacacaa aattcatctt aatcctcctt attccttgcc ttctaactta
    47041 ctaatatcaa ttcacaatgc atctgccatt tcttgattag tatgcaattc cagtttttca
    47101 ggcaactcaa ctgctatatt ttctggttcc tcacctttta aaacacgaat cagcatgcga
    47161 gctgtctgtc ttcccaattc ttcataattg gttccgtagt tatacaagcc accaacagca
    47221 atcatttctg ttgaaccacc gaatactgga accttgtgtt taatagaaac ctgcttaact
    47281 gtttccatgg ttgacgaaat gatattatca gttgggacaa aaaccatatc cacctctgcc
    47341 atcaaactct ctgctgccgc cttgacgtta ttactgtcca agattgtttt ttcaacaacg
    47401 gtaaagcctt tttctttcag aaatcgctta gcttcatcct tttaaacaac cgaattgggc
    47461 tcgctctgag tatagaggat tccaatcgtt ttagccttta gcaacacttt ctttatcaaa
    47521 ttgatttggg ttgaaatggc atctgatgac tgatcgcttg tcccagttac attgccccca
    47581 ggatgttctc ttgactcaac caacttggca ctgactgggt ctgttacagc tgaaaagata
    47641 actggtgtcg tttgcgtcgt attggccaag ctctgagcag aaggccttgc gatggctaga
    47701 acgacatcgc tggattctgc tagttgctgg gaaatcgttt ttagatttct ttgttctccc
    47761 tgtgcatttt gcaaatcaat ctcaatattt tccccctcaa catagccttg tttggccagc
    47821 tcatccacaa atccttctct agtagcgtct aaggactggt gggtaataaa ctgcgaaata
    47881 ccgatacgaa agacatctct tttcttatct gccttcaact gatgcaaact gatcagagaa
    47941 gtcaagagga tccccactac caagaggggg ctagtgattt tcgaacaact ttcataatga
    48001 aactccttct cagaaaagat aaaacaaaaa aacgcctaga tactatctaa acgggtactt
    48061 gaaataacct aacaagttac aacttagctg gtccatttag atattcatct atatggacca
    48121 caatcaaaaa tcttagccac atagatacaa acaaattgct tgaactgttt gcatccatgg
    48181 tgacatgtct acaaacacta tctaaagtag ctaaagtaaa agttttgatt catagttacg
    48241 gcttgtctct tattcatttc tttttctgct ttcttttttg atccttccta tcctaccacc
    48301 tttttcatca cctgtcaata atatttcaaa attttttaaa atttttcgaa aattctttca
    48361 g
//
LOCUS       DAVBCR010000006        46399 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCR010000006 DAVBCR010000000
VERSION     DAVBCR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.006"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            488..1531
                     /locus_tag="VQ508_000302"
     CDS             488..1531
                     /locus_tag="VQ508_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2489363.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1698..3785
                     /gene="topA"
                     /locus_tag="VQ508_000303"
     CDS             1698..3785
                     /gene="topA"
                     /locus_tag="VQ508_000303"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2489364.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4006..4365
                     /locus_tag="VQ508_000304"
     CDS             4006..4365
                     /locus_tag="VQ508_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2489365.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4458..5090
                     /locus_tag="VQ508_000305"
     CDS             4458..5090
                     /locus_tag="VQ508_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2489366.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQLGVTQLHGTKVVF"
     gene            5134..5790
                     /locus_tag="VQ508_000306"
     CDS             5134..5790
                     /locus_tag="VQ508_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2489367.1"
                     /translation="MFEIFKSYQFNQEKAHAFGFVVNSGVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETDDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5809..6441
                     /locus_tag="VQ508_000307"
     CDS             5809..6441
                     /locus_tag="VQ508_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2489368.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6469..6903
                     /locus_tag="VQ508_000308"
     CDS             6469..6903
                     /locus_tag="VQ508_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET2489369.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENACEMGRSVSYHDMPSV"
     gene            6915..7952
                     /gene="leuB"
                     /locus_tag="VQ508_000309"
     CDS             6915..7952
                     /gene="leuB"
                     /locus_tag="VQ508_000309"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2489370.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADVILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMISNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDEERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7949..8218
                     /locus_tag="VQ508_000310"
     CDS             7949..8218
                     /locus_tag="VQ508_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2489371.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8222..8581
                     /locus_tag="VQ508_000311"
     CDS             8222..8581
                     /locus_tag="VQ508_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2489372.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8743..9311
                     /locus_tag="VQ508_000312"
                     /pseudo
     CDS             8743..9311
                     /locus_tag="VQ508_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9405..9470
                     /locus_tag="VQ508_000313"
                     /pseudo
     CDS             9405..9470
                     /locus_tag="VQ508_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9633..11564
                     /locus_tag="VQ508_000314"
     CDS             9633..11564
                     /locus_tag="VQ508_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2489373.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            11551..12867
                     /locus_tag="VQ508_000315"
     CDS             11551..12867
                     /locus_tag="VQ508_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2489374.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            13042..14028
                     /locus_tag="VQ508_000316"
     CDS             13042..14028
                     /locus_tag="VQ508_000316"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2489375.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            14092..14163
                     /locus_tag="VQ508_000317"
     tRNA            14092..14163
                     /locus_tag="VQ508_000317"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14125..14127,aa:Arg,seq:ccg)
     gene            14346..15044
                     /locus_tag="VQ508_000318"
     CDS             14346..15044
                     /locus_tag="VQ508_000318"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2489376.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            15035..18574
                     /gene="smc"
                     /locus_tag="VQ508_000319"
     CDS             15035..18574
                     /gene="smc"
                     /locus_tag="VQ508_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2489377.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18571..19365
                     /locus_tag="VQ508_000320"
     CDS             18571..19365
                     /locus_tag="VQ508_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2489378.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPAAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19365..20183
                     /locus_tag="VQ508_000321"
     CDS             19365..20183
                     /locus_tag="VQ508_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2489379.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFADLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20187..21464
                     /gene="ftsY"
                     /locus_tag="VQ508_000322"
     CDS             20187..21464
                     /gene="ftsY"
                     /locus_tag="VQ508_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2489380.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLATVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21510..22997)
                     /locus_tag="VQ508_000323"
     CDS             complement(21510..22997)
                     /locus_tag="VQ508_000323"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2489381.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(23146..23886)
                     /locus_tag="VQ508_000324"
     CDS             complement(23146..23886)
                     /locus_tag="VQ508_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2489382.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23886..26051)
                     /locus_tag="VQ508_000325"
     CDS             complement(23886..26051)
                     /locus_tag="VQ508_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2489383.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26224..27177
                     /locus_tag="VQ508_000326"
     CDS             26224..27177
                     /locus_tag="VQ508_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2489384.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27254..29242
                     /gene="uvrB"
                     /locus_tag="VQ508_000327"
     CDS             27254..29242
                     /gene="uvrB"
                     /locus_tag="VQ508_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2489385.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARIEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            29252..29815
                     /locus_tag="VQ508_000328"
     CDS             29252..29815
                     /locus_tag="VQ508_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489386.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29781..30269
                     /locus_tag="VQ508_000329"
     CDS             29781..30269
                     /locus_tag="VQ508_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489387.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30343..30810
                     /locus_tag="VQ508_000330"
     CDS             30343..30810
                     /locus_tag="VQ508_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2489388.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30807..31322)
                     /locus_tag="VQ508_000331"
     CDS             complement(30807..31322)
                     /locus_tag="VQ508_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2489389.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31334..31861)
                     /locus_tag="VQ508_000332"
     CDS             complement(31334..31861)
                     /locus_tag="VQ508_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2489390.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(32139..32702)
                     /locus_tag="VQ508_000333"
     CDS             complement(32139..32702)
                     /locus_tag="VQ508_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2489391.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32686..33237)
                     /gene="coaC"
                     /locus_tag="VQ508_000334"
     CDS             complement(32686..33237)
                     /gene="coaC"
                     /locus_tag="VQ508_000334"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2489392.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(33249..33938)
                     /gene="coaB"
                     /locus_tag="VQ508_000335"
     CDS             complement(33249..33938)
                     /gene="coaB"
                     /locus_tag="VQ508_000335"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2489393.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            34168..35838
                     /locus_tag="VQ508_000336"
     CDS             34168..35838
                     /locus_tag="VQ508_000336"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2489394.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            36166..37341
                     /gene="mutY"
                     /locus_tag="VQ508_000337"
     CDS             36166..37341
                     /gene="mutY"
                     /locus_tag="VQ508_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2489395.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            37396..38100
                     /locus_tag="VQ508_000338"
     CDS             37396..38100
                     /locus_tag="VQ508_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2489396.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            38093..39442
                     /gene="vicK"
                     /locus_tag="VQ508_000339"
     CDS             38093..39442
                     /gene="vicK"
                     /locus_tag="VQ508_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2489397.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            39444..40253
                     /locus_tag="VQ508_000340"
     CDS             39444..40253
                     /locus_tag="VQ508_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2489398.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            40344..40586
                     /locus_tag="VQ508_000341"
     CDS             40344..40586
                     /locus_tag="VQ508_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2489399.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            40588..40842
                     /locus_tag="VQ508_000342"
     CDS             40588..40842
                     /locus_tag="VQ508_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2489400.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            40889..41662
                     /locus_tag="VQ508_000343"
     CDS             40889..41662
                     /locus_tag="VQ508_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2489401.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(41922..42908)
                     /locus_tag="VQ508_000344"
     CDS             complement(41922..42908)
                     /locus_tag="VQ508_000344"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2489402.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            43102..45570
                     /gene="gyrA"
                     /locus_tag="VQ508_000345"
     CDS             43102..45570
                     /gene="gyrA"
                     /locus_tag="VQ508_000345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2489403.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            45570..46313
                     /gene="srtA"
                     /locus_tag="VQ508_000346"
     CDS             45570..46313
                     /gene="srtA"
                     /locus_tag="VQ508_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2489404.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT        14346 a         8147 c        10292 g        13614 t
ORIGIN      
        1 caaggtgcac tgttatagat tcaatacact ataatttagt gtaattgaga aaggagaaat
       61 gattgtgatt gatgttggct aggttatgtt caatgattcc taccgtctca aatcttgtca
      121 gtaaggaaaa ataaattctt caaaagtaga gattacaagg cttgtttaag aaagaattca
      181 aagaccttga caaataaaaa taaaatggtt attataaaaa atggtctgaa atagatgatg
      241 atacttttcg aaaatctttt tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc
      301 tacggttagc ttccgagttt gattttcatt tactagaaat gaaactgatg agagatatca
      361 gtagacattt gagtcaggat attatggaaa atgataaaaa gagctcgtga gattggcata
      421 tcagactact aaagtattga gtttgttagg attttagcga ctagttagct gggaaaggaa
      481 gatatttgtg acaaataata aactgtattc gttgatagaa tttagaaata aaatatatga
      541 agaattagaa ctttccagaa gtgatttagc gattttacta tgtgccatgc ttatcgcctc
      601 tatcggatta aatatggatt cgactcccgt gattattgga gccatgttaa tctctccttt
      661 gatgacacct attctgggag tggggctctc tctagctata tttgatttta aattgttaag
      721 aaaatctttt aaaatattag ctattcaaat tcttgccagt ttaatagctt caacacttta
      781 tttttatctt tctcccattt cgtatgctag ttcggaaatt gttgctagaa cctctccgac
      841 tatttgggat gttctcattg cttttgtagg agggatagca ggtattattg gtgctaggaa
      901 aaaagagacc aataatattg ttcctggtgt tgctattgca accgccttga tgcctcctct
      961 ttgtacagta ggttatgcta ttgcttctgc taatctaaaa tttatcatag gctcctctta
     1021 cctattcctc atcaattgta gctttattgt cattgcgact tatataggtg ttaggttgat
     1081 gatggttaag aaacattatt ttaaagataa tgaagaagac tctaaaatgc gtaggatttt
     1141 gcttctagtt gctgttttgc tgatgattcc gagtttcatc tctgcaacga ctttagtgag
     1201 agaaacgttg aaaaaagagt cccttaagaa atttatatca gagcagtttc aggggcataa
     1261 tattttgaaa aaaacctatt ctaaaaagac tcatacccta aagctaacca tttcaggaaa
     1321 ttatttgaca gaagaagaac tcgatatgat ttcaagtaag agaggtgact atggtttaag
     1381 tgatgtttct gttcaagttt cacaattgtc tgattcagaa caacttagta aggaagaact
     1441 ggtggagtat ttcttccagt atatcaagga taaggaagca aaagaaaagg aaaaagctaa
     1501 taagttttat acagagtctg aggagcaata atttcttgag aatagctggt ttctctcgtg
     1561 agtcttctat gtatatcaaa ggaagactga ggttttaagt ataaaacttt tcttctatta
     1621 tagtagctaa gtcttgacag ggttcaaaaa atggtttaca ctttataaag tttattactt
     1681 tgaaaaggtg tgatactgtg gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa
     1741 atctagtcat cgtggagtcg cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa
     1801 actacaaggt tttagccagt gtcgggcata tccgtgattt gaagaaatcc agtatgtccg
     1861 tcgatattga aaataattat gaaccgcaat atatcaatat ccgaggaaaa ggccctctta
     1921 tcaatgactt gaaaaaagaa gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg
     1981 accgtgaagg agaagcgatt tcttggcatt tggcccatat tctcaacttg gatgaaaatg
     2041 atgccaaccg tgtggtcttc aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag
     2101 aacctcgtaa gatcgatatg gacttggtcg atgcccaaca agctcgtcgg atcttggatc
     2161 gcttggtagg gtattcgatt tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag
     2221 caggtcgcgt tcagtccatt gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg
     2281 ccttccagcc agaagaatac tggacagttg atgctgtctt taaaaaggga accaaacaat
     2341 ttcatgcttc cttctatgga gtagatggta aaaagatgaa actgaccagc aatgacgaag
     2401 tcaaggaagt cttgtctcgt ctgactagta aagacttttc agtagatcag gtggataaga
     2461 aagagcgcaa gcgcaatgct cctttaccct ataccacttc atctatgcag atggatgctg
     2521 ccaataaaat caatttccgt actcgaaaaa ccatgatggt tgcccaacag ctctatgaag
     2581 gaattaatat tggttctggt gttcaaggtt tgattaccta tatgcgtacc gattcgactc
     2641 gtatcagtcc tgtagcgcaa aatgaggcgg caagcttcat tacggatcgt tttggtagca
     2701 agtattctaa gcacggtagc aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg
     2761 ctattcgtcc gtcaagtgtc tttaatacac cagaaagcat cgctaagtat ctggacaagg
     2821 atcagctcaa gctatatacc cttatctgga atcgttttgt ggctagccag atgacagcgg
     2881 ccgtttttga taccatggct gttaaattgt ctcaaaatgg agttcaattt gctgccaatg
     2941 gtagtcaggt taagtttgat ggttatcttg ccatttataa tgattctgac aagaataaga
     3001 tgttaccgga catggttgtt ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac
     3061 atttcaccca accgcctgcc cgttattctg aagcaacact gattaaaacc ttagaggaaa
     3121 atggggttgg acgtccatca acctatgcgc caaccattga aaccattcag aaacgttatt
     3181 atgttcgcct ggcagccaaa cgttttgaac cgacagagtt gggagaaatt gtcaataagc
     3241 tcatcgttga atatttccca gatatcgtaa acgtgacctt cacagctgaa atggaaggta
     3301 aactggatga tgtcgaagtt ggaaaagagc agtggcgacg ggtcattgat gctttttaca
     3361 aaccattctc taaagaagtt gccaaggctg aagaagaaat ggaaaaaatc cagattaagg
     3421 atgaaccagc tggatttgac tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc
     3481 gttttggtaa attctacgct tgtagcaatt tcccagattg ccgtcatacc caagcaatcg
     3541 tgaaagagat tggtgttgag tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa
     3601 ccaagcgtaa tcgcctattc tatggttgca atcgctatcc agaatgtgaa tttacctctt
     3661 gggacaagcc tgttggtcgt gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag
     3721 tccgtggtgg tggcaagcag gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta
     3781 aataagagga ggggcccgaa agtaaatttc aggctccttt tggttagaac ttgacaaaat
     3841 acatcatttt atgcgaaact agaagaagat tattttatac tcaatgagaa tcaaagagca
     3901 aattagaaag ctagtcgcag gttgctcaaa gcactacttt gaggttgaag atggaagctg
     3961 acctggtttg aagagatttt cgaagagtat taactaggag aagttatgcg tcttatctat
     4021 ctgataattg gttttttatc actggccttg gctattgttg gggttgtttt acccttgttg
     4081 ccgacaacgc ctttcctttt gttgtctatt gcttgtttct ccagaagttc caagcgtttc
     4141 gaagactggc tttatcatac caagctctat caaacatatg tagctgattt tcgcgagact
     4201 aagtctatta cgcgtgaacg aaagaaaaaa atcatcgtct ctatctacgt cttgatggga
     4261 atttctattt attttgcacc tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc
     4321 tttattactt attatctctt caaggtcatt ccagacaaag aatagttaaa acagtagtta
     4381 tttgccttga taaaattgaa agcatattca taacaatatg atataataaa tttgaagtaa
     4441 tattcaagga gaatcaaatg atttacgaat tttgtgctga aaatgtgact ttacttgaaa
     4501 aagcgatgca ggctggagct cgtcggattg aactctgtga taatctagca gttggtggga
     4561 caacacccag ctatggagtg actaaggcag cggttgaact ggcagctaac tacgatacaa
     4621 ccatcatgac catgattcgg ccacgtggtg gtgactttgt ctataatgac ctagaaattg
     4681 ctatcatgct agaagacatt cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg
     4741 gaactttaac tgctgataaa aagttggata agcctaatct ggaaaagtta attgctgcat
     4801 caaaaggaat ggaaattgtc tttcacatgg cctttgatga actaagtgat gaagatcaac
     4861 cggaagctat tgactggctc agtcaagccg gtgtcactcg tatcctaact cgtgctggtg
     4921 tgtctggcga ctccttagaa aaacgttttg ttcactatca cagaattttg gagtacgcta
     4981 aaggtaaaat tgaaattcta ccaggtgggg ggattgacct tgaaaaccgt caaaccttta
     5041 tcgaccagtt gggggtaaca caattgcatg gtactaaggt tgttttttaa aaaatagaaa
     5101 ggaactgcta gctttgggta gcagttttca cttatgtttg aaatttttaa atcctatcaa
     5161 tttaatcaag aaaaggctca tgcttttggt tttgtagtaa atagtggagt ctggacctat
     5221 agttgccaga ttttgcaagg tgactttgtc atgactgtgt ccatcactgc tgataatgtg
     5281 aactttcaag tctttgacca agagactgat gacctctatc ctcacgttca tatggaaagc
     5341 atgaggggaa gttttgtcgg aaatgtccgt gaggcttgtc tggagattct ttaccagatt
     5401 cggaaggctt gttttgatgt gcaagatttt atctgtcatc agactaagcg tattatgact
     5461 caagttcagg aaaagtatgg aaaccagttg gagtatctgt gggaaaaatc gcctgataca
     5521 gctgtattgc gccatgaagg caatcaaaag tggtatgccg tcttgatgaa aatctcttgg
     5581 aataagctgg aaaagggcag agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa
     5641 gtagctaatt tactttcaca aaaggggatt tatccagcct ttcatatgag caagcgctac
     5701 tggattagtg tgtcccttga tgatacttta tcagatgaag aagtactgga attgatagaa
     5761 aaaagttgga acttaacctc taaaaaatga aatattttaa taattttcat gaactttcaa
     5821 ttagctaaat attctttact gaagaaattt tcagaaaata tagaatttac cacaccaggg
     5881 gaatatggtg ccatcttcaa atacctgatt gagaatgtca agacggatcg tcagatcatc
     5941 tgttcgcctc actgtcatga tgacctcgga atggcagtgg caaatagcct tgctgctgtc
     6001 aagaatggtg caggacgtgt tgaagggact atcaatggta ttagggagcg agctgaaaat
     6061 gctgctttgg aagaaattgc agtggctctc aatattcgcc aagattacta ccaagtagaa
     6121 accagtattg tcctaaatga gaccatcaat acgtcagaaa tggtttctcg cttctctggt
     6181 attccagttc ctaaaaacaa agccgtcgtt ggtggcaata ccttctccca tgaatctggt
     6241 attcaccaag atggagtcct taaaaatcct ctcacttatg agatcatcac acctgaattg
     6301 gttggtgtta agattccgct tggaaaatta tctggtcgcc atgcttttgt tgagaaactg
     6361 agagaattgg ccctagattt tacagaagag gatatcaaac cactctttgc taagttcaag
     6421 gcactggccg ataagaagta agaaatcaca gatgcagatc ctcgagcttt ggtagctgga
     6481 accatggttg aaaatccaga aggcttccac tttgatgatt tacaacttca aactcatgca
     6541 gataatgaca ttgaagcgct cgttagccta gccaatatgg atggtgagaa agtcgaattt
     6601 aatgcgacag ggcaaggttc cgttgaagca atctttaacg ctatcgataa gttctttaac
     6661 caatctgttc gtttggtgtc ctacactatc aatgcggtaa cagatggaat cgatgcccag
     6721 gatcgggttt tggtcactgt tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg
     6781 cttgattttg atgtgttgaa ggcttctgct attgcctata taaacgctaa tacctttgtt
     6841 caaaaagaga atgcatgtga gatgggacgc agtgtttctt accacgatat gcctagtgtg
     6901 taaaggagaa ggctatggca aagaaaatag tagctctagt aggagacgga attggcccag
     6961 aaatcatgga ggctggttta gaagttctgg aggctctagc tgaaaaaaca ggttttgact
     7021 atgagattga cagacgaccg ttcggaggtg cagatattga tgcagcagga cctcccttac
     7081 ctgatgaaac ccttaaggca agtagggaag cagatgttat cctactagca gctatcggta
     7141 gtcctcagta tgatggagca gcggttcgcc ctgaacaagg cctgatggct ctccgtaagg
     7201 aactcaatct ttacgctaat attcgtcctg taaaaatctt tgacagtctc aagtatttgt
     7261 caccacttaa accggaacga atttctggtg tagactttgt cgtggtgcgt gaattgacag
     7321 gcgagattta ctttggagat catattcttg aagagcgcaa agcgcgtgat atcaacgact
     7381 atagctatga ggaagtggag cggattattc gcaaagcctt tgaaattgca agaaatcgca
     7441 gaaaaatcgt tactagtatc gataagcaaa atgttctagc gacctcaaaa ctctggcgga
     7501 aagtagctga ggaagtcgca caggatttcc cagatgtaac cttggaacac cagctggtag
     7561 actcagctgc tatgcttatg attagcaatc ctgctaagtt tgatgttatt gtaacggaga
     7621 atctttttgg agatatttta tctgatgaat caagcgtctt atctggtaca cttggggtta
     7681 tgccatcagc cagtcattct gaaaatggac caagtctcta tgaacctatt tacggttcag
     7741 cacctgatat tgcaggtcaa ggaattgcca atcctacttc catgatttta tcagttgcca
     7801 tgatgttgag agatagtttc ggacgttatg aggatgaaga gcgtatcaaa cgtgctgttg
     7861 agacaagtct ggcggcagga attttaacga gagatatagg ggggcaggct tcaacaaagg
     7921 aaatgacgga agctattatt gcaaggttat gaagttagac gaaaaaatta ctctagtcct
     7981 tttgatttgg aatgtcatca ttttcttgat ttatggcatt gacaaattca aggcaagaag
     8041 aagaacttgg cgcatccaag agaaaatctt actcatttta gcccttactt gtggtggttt
     8101 tggggcctgg ttagctggaa tcacttttca ccacaagact cgaaaatggt actttaaaac
     8161 agtttggttt cttgggatgg tgaccacact agtagcctta tattttattt ggaggtaatg
     8221 gatggcaggg tcttcgaggg aacacgctgc ttgggctcta gcgaactatg gttttaaggt
     8281 cgtgattgca ggaactttcg gtgacattca ttacaataat gaactcaata atggcctgtt
     8341 gccaatcgtt cagcctagag aggttagaga gaaactagcc cagctaaaac caaccgacca
     8401 tgtaactgtg gacttggaac aacaaaaaat catctcacca gttgaagaat tcaccttcga
     8461 gatagatagc gagtggaaac ataaactcct aaatagtttg gatgatatcg gtattacctt
     8521 gcagtatgaa gagttgattg ctgcttatga aaaacaacga ccagcctact ggcaggatta
     8581 gaaaaaatag aaaaggaaat atagtaaact gaaataagat gtaaacaaat gaattagagc
     8641 ttaacatcca tttccagcaa ttttttagaa actacagtgg actattctgg attcaacaca
     8701 ttataaaatt atgacaagac acattcacaa gaaggctacg acattttaaa aggtgagggc
     8761 ggatgtatcg tttgccctac taaagttggt tacattatca tgaccagtga caaggcagga
     8821 cttgagcgta agttcgcagc caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc
     8881 ggtagcattg atgaactttg cgctttagcg caactcaacc cagaaattga agcattctac
     8941 taaaaacatt gggatgaaga tattcttctt ggttgtatcc ttccttggaa accagaagcc
     9001 tttgaaaaac tcaaagcata cggggatggc cgtgaagaac ttatgactga tgtacgtggt
     9061 actagctgtt ttgttatcaa gtttggaaaa gcaggtgaac aattggctgc caagctttgg
     9121 gaagaaggta aaatggtcta cgcctcatct gcttcaatga caaaacgatt gaaactcgct
     9181 atgagcaagg tgtaatggtg tctatggtcg ataaggacgg caaactcatc ccagaacaag
     9241 gaggagcacg ttcaacttca ccagctccag ttgtgatccg taaagggctt gacattgata
     9301 tagtaaaatg aagtaagagc aggacaaatt aatcaggaca gtcaaatcga tttctaacaa
     9361 tgttttagaa gtagaggtgt actattctag tttcaatcca ctataaaatc atgatgcacc
     9421 tgtcagatac ttttaactca tgggactacc gtcaggttga gtattattag gatagagaag
     9481 aagtctagtg ttaaggacat tacagctcat aatactaggc ttttttatag aaattctttt
     9541 ctttttctat aggatatggt attctatgta gaaaatatat gttaataagt aatgccaata
     9601 tttaaacatc attagtaaaa ggagttagat tgatgaataa aagaaaagtt agtttagaag
     9661 atttttataa atggtatagt ctaaataaag aagagttatt aaataaggca actgttggtg
     9721 aaaagtttaa tgataaatta aaagaagagt ttctccagga atggcctttg gataggattt
     9781 taacaatgtc aatcgatgaa tatgtaatag gaaagggaca gcaaaataag tctttatgct
     9841 acgctcttga gaagggaaaa tacaaaaatc tatttcttgg aatttctggt ggctcagctt
     9901 caaaatttgg tatttattgg aataaaaaaa caaacaaata taaagatcaa gctaataatg
     9961 agatttcaga gttggatcag cgattttcaa aattaaaatc agatttgtat gaaattatca
    10021 aagaaggtat tcgttttaac tttgaaaatc ctatttttga tatgaaaaga tcaacaaatg
    10081 aatttattgg tcgttctgct atggtgacaa aattactttg tatctatact gagggagatc
    10141 ctttctttgg tgtaaatatt aatagtcaga aagaattttg gaaccacttt gtttctcaga
    10201 caaatcaagg tggaccttat ctgcaaaatc ataaaataat tgaactgatg tccaaaactt
    10261 atcctgagtt ggagccatcg aaattaggaa ctatgctttt tgagtattct aagcttttta
    10321 tggaaaataa ggaagacaat agtacaatgg attcatcaaa caattttcgt catcaattaa
    10381 ctcaatctct attaaagtct ccaaacctta tccttcgtgg tgctcctggc acaggaaaga
    10441 cttatcttgc taaagaaatt gccaaagaat taacggatgg caacgaagat caaatcggat
    10501 ttgtacaatt tcacccatca tatgattata cggattttgt agaaggttta agaccagtat
    10561 caaatgggga tggagctatt gagtttaggc tacaggacgg tatttttaaa gatttttgtc
    10621 agaaagcaaa agaaacccaa ttgattggag gacaagataa ttttgatgag gcttgggatt
    10681 cttacttaga atatataaat gttgctgaag aaaaagaata tataacaaaa acatcttact
    10741 tatctgttaa tagtagacaa aatttgtcag taaattatga tagtggtgtt ccaggatggt
    10801 cactacctag caaatatgtt tacgagttgt ataaagataa aaattataat aagcaagaat
    10861 actacaaaag tggtggaaaa actgtcctag aaacattgag aaagagattt ggtttgaaag
    10921 actatgtttc cccaacagaa attgatactg ataagaaatt cgttttcatc atcgatgaaa
    10981 tcaatcgtgg ggagatttct aagatttttg gcgaactctt tttctctatc gaccctggct
    11041 atcgtggtga aaaaggaagt gtttctaccc aatatgcaaa tctacacgaa actgatgaaa
    11101 agttctatat ccccgaaaat gtttacatca tcggaactat gaatgatatt gatcgttcag
    11161 tggatacctt tgattttgct atgcgtcgtc gttttcgttt tgttgaagtt actgccgagg
    11221 gtcaagttgg catgttggat aaagagctga atatccatgc agaagaagca aaaattcgtc
    11281 taagaaactt gaacgctgct atcgaaaatg ttcaggaatt aaacagtcat tatcatattg
    11341 gaccaagtta ttttcttaag ttgaaggatg tagattttga ctatgaatta ctctggtccg
    11401 attatattaa gcctctccta gaagactact tgcgaggttc ttatgatgag gttgaaactt
    11461 tggaaacttt gaaaaaagca tttgaactga caaataatga gcaaaaagat caggcagtag
    11521 ctgatgacaa tgaaagcgat gaaaacgatg atgcggatta ctgataatca acacaagatt
    11581 attaaagaaa aatttgttga agaatatcct aaactaagca atcttctttt agacagaacc
    11641 ttggaaagtc tatcccaaga tgaacgtatt ttcatttttc caaatgattt gactcatact
    11701 ccagatttgg ataaaaaaca aaagattttg gaaacgatca atcaggaaat caagacagga
    11761 aatgtgattg gttttcttgg atatggtcag gaaagattaa cgatttcctc acgattttct
    11821 gatgagagta atgaccattt tttgcattat ctcttaaaca aggttcttca tatcaatctc
    11881 actagtttag atgttgcttt gtctcgtgaa gagaggcttt atcaactttt gatgtatctc
    11941 ttccccaagt atctacaagc tgctattcga aaaggtcttt ataaggaata tcatcgattt
    12001 tctcataacg acagtcatgt taagggagtg attgatgtaa gaaaccatct caagaaaaat
    12061 cttcctttca cgggaaatat tgcctacaca acgagagagt tcacctatga taatcccctc
    12121 atgcagttgg tccgtcacac tattgaatac attaagaatc agaaaagcat tggtcaaggg
    12181 gtactagata atctctcaac tagtcgtgaa aacgtatctg aaatcgtgcg tgtaacgccc
    12241 tcttataaac tagctgatcg tgctaagatt attcggggaa atcaatctaa acctatacgt
    12301 catgcatact ttcacgagta cagaaactta caagaacttt gtctgatgat cctaaaccaa
    12361 gaaaagcacg gtttagggta tcaagatcaa aaaatctatg gtattctctt tgatgttgcc
    12421 tggctttggg aagagtatgt ttacaccttg ttgccaaaag gttttgtaca tcccagaaat
    12481 aaggataaga cggatggaat ttcagtattt tctgttggga aacgaaaagt atatccagat
    12541 ttttatgaca gagaacgaaa gattgttcta gatgcaaaat ataaaaaact ggaattgact
    12601 gaaaaaggaa tcaatcgtga ggacttattc cagctgattt cctattctta tattttaaaa
    12661 gctgagaagg ctggacttgt ttttccgagc aaggacaagg ttattgataa tgagatggga
    12721 aacctagcag gttatggagc tttgttgaag aaatggtcta tccaaattcc tcagaagtct
    12781 tcatcttata gtgcattttg taaaatgatg gaaaattcag aagaaaattt taaagcgatt
    12841 attgatgaag aagtggggag aaaataattt cccctctttc ttttattttt aacacaagta
    12901 aaacccgaac aatatatttc aattcataaa ataatttgac aaaaactgta ctttggttta
    12961 taataaatta aggaaacgtc ggaaaagacg tagtgatgcg agagcatagg taggtcatta
    13021 caaaagaaac gagacatcga tatgttaaat gaatttccaa tttttgatta cgaagatatt
    13081 caattgattc caaataaatg tgtcattaaa agccgtgcag aagcggatac aagtgtcact
    13141 ttaggaaatc acacctttaa actacctgtt gtgccagcga atatgcagac gattttggat
    13201 gaaaatgtag cagagcaact ggctaaaggt ggttacttct acattatgca ccgttttgat
    13261 gaggcaggac gcattccttt tattaaacaa atgcacgatc aagggctcat tgcttccatc
    13321 tctgtcggtg ttaaggacta tgagtatgat tttgttagcc agctcaagac tgatgctcct
    13381 gagtatatca ctattgatat tgctcatggt catgcggata gcgtgatttc tatgattcaa
    13441 catatcaaga aagaattgcc agataccttt gtcattgctg gaaatgtggg aacaccagaa
    13501 gctgtgcgtg aattggaaaa tgctggtgcg gatgctacta aggtcggaat cggtcccggt
    13561 aaggtttgta tcaccaaggt taagactggt tttggtacag gtggttggca gttggctgct
    13621 ctacgctggt gtgccaaggc tgcgcgtaaa ccgattatcg ctgatggagg aattcgtact
    13681 cacggtgata ttgctaagtc tatccgcttc ggtgctagca tgatcatgat tggttccctc
    13741 tttgcaggac acattgaaag tccagggaaa acgattgaag tcgatggtga acagttcaaa
    13801 gaatattatg gttcagcctc acaatatcaa aaaggtgctt acaaaaatgt ggaaggcaaa
    13861 cgtatcttgc ttcctgctaa aggtcatctg caagacactt taactgagat ggaacaagac
    13921 cttcaaagtg ctatttctta tgcaggtgga cgtcaggttg ctgaccttaa acacgttgat
    13981 tatgtgatcg tgaaaaactc catctggaat ggggatgctt cccactaaga cggggtcttt
    14041 gaccaaaaaa cttgttatta gagcaaattt ctgttataat aaaacaagtt tccaccctta
    14101 gtgtaatgga tatcacgtaa gattccggtt cttgagatgg gggttcgatt ccctcagggt
    14161 ggatgtaaac atcctaaaaa gcctttaata gggctttttt ctttatcctg acccaaattt
    14221 gccgctaaaa ctgagaatct ctgtctaaag aattcccaaa tgctttccag tcccgtttta
    14281 gagtgactca gggggctttt tttgatataa taaaaaggac tgttatcagt tagaaagagg
    14341 ttggtatgaa agaattacaa actgtactaa agaatcattt tgcaatcgaa tttgcagaca
    14401 aaaatttact ggaaactgcc tttactcata cgagttatgc caatgagcac cgcctcttaa
    14461 aaatttcaca caatgaacgc ttggaatttt taggagacgc tgttctacag ttattgattt
    14521 cagaatatct atataaaaaa tatcctaaaa agcctgaagg tgacctatca aaactccgtg
    14581 ctatgattgt ccgtgaggag agtttagctg gttttgcgcg tgattgccag tttgaccagt
    14641 ttatcaagtt gggtaaaggg gaagaaaaat caggtggtcg caatcgtgac accattcttg
    14701 gtgatgcctt tgaagccttt cttggtgccc ttcttttgga taaggatgtg gccaaggtca
    14761 aggaatttat ctatcaagtc atgattccta aggttgaagc aggcgaattt gagatgatta
    14821 cagactataa aacccatctc caagagttac ttcaggtcaa tggtgatgtg gctattcgtt
    14881 atcaggtgat ttctgaaaca gggcctgctc acgataaggt ctttgatgta gaagttcttg
    14941 ttgaaggtaa gagcatcggt caaggccaag gtcgttctaa gaaattagca gagcaggaag
    15001 ctgccaaaaa tgccgttgag aaggggctgg attcatgtat ttaaaggaaa tcgaaattca
    15061 ggggttcaag tcttttgctg ataagaccaa ggtcgttttt gaccaaggtg tgacggcagt
    15121 tgttggaccc aatggatctg gaaagtccaa tattacagaa agtctgcgtt gggctttggg
    15181 ggagtctagt gtcaagagtc tccgtggggg caagatgccg gatgtcatct ttgctggaac
    15241 agaaagtcgc aaaccgctca attatgcttc tgtagttgtg actctggata atcatgacgg
    15301 atttatcaag gatgcaggtc aagaaatcag ggtggaacgc catatctatc gtagtggaga
    15361 tagcgaatac aagattgacg gcaagaaagt ccgtctgcgt gatattcatg accttttctt
    15421 ggatacaggt ttgggacgag attccttctc tattatttcc caagggaagg ttgaggagat
    15481 ttttaattcc aagtctgagg aacgacgagc tatttttgaa gaagctgctg gagttttaaa
    15541 atacaagact cgcagaaaag aaaccgagag taaactgcag caaactcagg ataatctgga
    15601 ccgcttagag gacattatct acgagttgga taatcaaatc aagcctcttg agaagcaagc
    15661 tgagaatgcc cgtaaatttc tagacttaga aggtcaacgc aaggctattt acttggatgt
    15721 attggttgct caaatcaagg aaaataaggc agaactagag tcgacagaag aagagttggc
    15781 tcaggttcaa gaactcttga tgagttatta ccaaaagcgt gaaaaattag aagaagaaaa
    15841 tcaaactctt aaaaagcaac gccaagattt acaggctgaa atggccaaag accaaggcag
    15901 tttgatggac ttgactagtc tgattagtga tttagaaaga aaattagccc tatcgaaact
    15961 ggagtctgag caagtggcct tgaatcaaca ggaggcacaa gctcgtttgg ctgctttgga
    16021 ggataagaga aattcactca gcaaagaaaa gtctgataaa gaaagctctt tagctctgtt
    16081 agagggaaat ctagtccaaa ataatcaaaa actcaatcgt ttagaagctg aattgctggc
    16141 tttctcagac gatcctgatc agatgattga gctcttacgt gaacgctttg tagctctttt
    16201 acaagaagaa gcggatgtct caaaccagtt gacccgtatt gagaatgagt tggaaaatag
    16261 tcgtcagctt tctcaaaaac aagcagatca actagaaaag ctgaaagagc aattagctat
    16321 agctaaagag aaggctagtc agcaaaagga agagcttgaa actgccaagg agcaggttca
    16381 gaaattattg gctgactacc aagctattgc caaggagcaa gaggagcaga aaacttccta
    16441 tcaagctcaa caaagccaac tctttgaccg tctggataat ctcaaaaaca agcaggccag
    16501 agctcaaagt ttggaaaata tcctgagaaa tcatagtaac ttttatgcag gtgttaagag
    16561 tgttctccaa gaaaaagatc gcctaggtgg gattattggt gcagtcagtg agcatctgac
    16621 ctttgatatg cattatcaaa ctgccctaga aattgcgctt ggagcgagta gccaacatat
    16681 catcgtagaa gatgaagagt cggcaaccaa ggctattgac ttccttaaac ggaacagagc
    16741 cggtcgtgca acctttcttc ctttgaccac tatcaaggcg cgtacgattt ctagtcagaa
    16801 ccaagatgct atcactgtaa gcccaggttt ccttgggatg gcagatgagt tggtgacttt
    16861 tgatactaga ctggaagcca ttttcaagaa cttgctagct acgacggcta tctttgatgc
    16921 cgtagaacat gcgcgtgcag cagctcgcca agttcgttat caggttcgta tggtgacatt
    16981 ggatgggaca gaattacgca cgggtggttc ctatgcgggt ggtgccaatc gccaaaataa
    17041 cagtattttc atcaagccag aactggagca attacaaaaa gaaattgctg cagatgaagc
    17101 aagcttgcgt tcagaagaag cggctttgaa gaccttacaa gaccagatgg ctacattgac
    17161 agaaagatta gaagtcatca aatctcaagg agaacaggca cgtattcagg agcaaggctt
    17221 gtccctcgct tatcagcaaa ctagtcagca agttgaagaa ctggaaactc tttggaaact
    17281 ccaagaagag gaaatagatc gtctttccga gggagattgg caagcggata aggaaaaatg
    17341 ccaagatcgt cttgctgcaa tcgccagtga caagcaaaat ctggaagctg agattgaaga
    17401 gattaagtct aacaaaaatg ccatccaaga acgctatcaa aacttgcagg aagagctagc
    17461 gcaagctcgt ttgcttaaga cagaactgca agggcaaaaa cgttatgaag ttgctgatat
    17521 tgaacgctta ggcaaggaat tggacaatct tgattttgaa caagaggaaa tccagcgcct
    17581 tcttcaagaa aaggttgata atcttgagaa ggttgataca gaattgctca gtcaacaggc
    17641 ggaagaagcc aaaactcaga aaacaaatct ccaacaaggt ttgattcgca agcagtttga
    17701 gttggatgat attgaaggtc aactggatga tattgctagt catttggatc aggctcgcca
    17761 acagaatgaa gagtggattc gcaagcaaac acgtgctgaa gccaagaaag aaaaggtcag
    17821 cgagcgcttg cgctatttac aaagtcaatt aacagaccaa tatcagatta gctatactga
    17881 agctctagaa aaggcgcatg aattggaaaa cctcaatctg gcagagcaag aggtgcagga
    17941 tttagagaaa gctattcgct cactggggcc tgtcaatata gaagctattg accagtacga
    18001 agaagttcac aatcgtctgg atttcctaaa tagccaacga gatgatattt tgtcagcgaa
    18061 aaatctgctc cttgagacca ttacagagat gaatgatgag gtcaaagatc gctttaaatc
    18121 aacctttgag gccattcgtg aatcctttaa agtgaccttc aaacagatgt ttggaggagg
    18181 tcaggcagac ttaatcttga ctgagggaga cttgttgaca gctggggttg agatttctgt
    18241 tcaacctcca ggtaagaaaa tccagtcgct taacctcatg agtggtggtg aaaaagcctt
    18301 atcagctctt gctttgcttt tttctatcat tcgagttaag actatcccat ttgtcatctt
    18361 ggatgaggta gaggccgcgc tggacgaagc caatgttaaa cgttttgggg attacctcaa
    18421 tcgatttgac aaggacagcc agtttatcgt cgtaacccac cgtaagggaa ccatggcagc
    18481 ggctgattct atctatggag tgaccatgca agaatcgggg gtctcaaaaa ttgtttcggt
    18541 taagttaaaa gatttagaaa gtattgaagg atgacaatta aactagtagc aacggatatg
    18601 gacggaacct tcctagatgg gaatggacgc tttgatatgg atcgtctcaa gtctctcttg
    18661 gcttcctaca aggaaaaagg gatttacttt gcggtggctt cgggtcgggg atttctgtct
    18721 ctggaaaaat tatttgctgg tgttcgtgat gacattattt tcatcgcgga aaatggcagt
    18781 ttggtagagt atcaaggtca ggacttgtat gaagcgacta tgtctcgtga cttttatctg
    18841 gcaacttttg aaaagctgaa aacgtcacct tatatagata tcaataaact gctcttgacg
    18901 ggtaagaagg gttcatatgt tctagatacg gttgatgaga cctatttgaa agtgagtcag
    18961 cattataatg aaaatatcca aaaagtagcg agtttggaag atatcacaga tgacattttc
    19021 aaatttacaa ccaacttcac agaagaaacg ctagaagctg gtgaagcttg ggtcaatgat
    19081 aatgtccctg gtgtcaaggc tatgacaact ggctttgaat ctattgatat tgttctggac
    19141 tatgtcgata agggtgtagc tattgttgaa ttagctaaaa aacttggcat cacaatggat
    19201 caggtcatgg cttttggaga caatcttaat gacttacata tgatgcaggt tgtgggacat
    19261 cctgcagctc ctgaaaatgc acgaccagag attttagaat tagcaaagac tgtgattggt
    19321 caccataagg accagtcggt tatagcttat atggagggct tataatggca gatataaaat
    19381 taatcgcatt ggacttggac ggtaccttgc tgactactga taaaaggctg acggatcgca
    19441 ccaaggcaac tttgaaagct gcgcgtgatc gtggtatcaa ggtcgtattg acaactggtc
    19501 gtcccttaaa agccatggac ttctttctcc atgagttagg gactgacgac cacgaagatg
    19561 agtataccat tacttttaat ggtggattag ttcagaaaaa tacaggagaa atccttgata
    19621 aaacagtctt ttcatatgat gatgtggcac gtttgtatga agaaacagag aaattatcac
    19681 tgcctcttga cgctatctca gaaggaactg tttatcaaat tcaatcggac caagaaagtc
    19741 tttatgccaa attcaatcca gctttgacct ttgttccagt ggactttgca gatctgtcta
    19801 gtcaaatgac ctacaacaaa tgcgtgactg cctttgctca agaacccttg gatgcagcca
    19861 ttcagaagat ttctccagaa ttgtttgacc agtatgaaat ctttaaatca cgtgaaatgt
    19921 tgctagagtg gtcaccaaag aatgttcata aagcaacagg cttggcaaaa ctgatcagcc
    19981 atcttggaat tgatcaaagt caagtgatgg cctgtggtga tgaagccaat gacctctcta
    20041 tgattgaatg ggcagggctc ggtgttgcta tgcaaaacgc tgttcctgaa gtcaaggctg
    20101 tcgcaaatgt agtgacgcca atgaccaacg atgaggaagc tgtcgcctgg gctatcgaag
    20161 aatacgtgct aaaggagaac taagatatgg gattgtttga ccgtctattc ggaaaaaaag
    20221 aagaacctaa aatcgaagaa gttgtaaaag aagctctgga aaatcttgat ttgtctgaag
    20281 atgttgatcc taccttcaca gaagttgagg aagtttctca ggaagaagca gaggttgaaa
    20341 ttgttgaaca agctgtgttc caagaagagg aaatccaaga cacagttgaa gaaagtctgg
    20401 atttagagcc agttgtagaa gtttctcaaa aagaagtcga agaatttcca cactcagaag
    20461 aagggaatac tgagtttcta gcgactgtag aagaaaataa ttctgaaatt cttgaagcag
    20521 aaaggcctca aacagaagaa actgttcagg aaaaatatga ccgcagtctt aagaaaactc
    20581 gcacaggttt cggtgcccgc ttgaatgcct tctttgctaa cttccgctct gttgacgaag
    20641 aatttttcga ggaactggaa gaactgttga ttatgagtga tgttggtgtc caagtcgctt
    20701 ctaacttaac ggaggaacta cgttacgaag ccaagcttga aaatgccaag aaacctgatg
    20761 cacttcgtcg tgtcatcatt gagaaattgg ttgagcttta tgaaaaggat ggtagctacg
    20821 atgaaagcat ccacttccaa gataacttga cagttatgct ctttgttggt gtgaatggtg
    20881 ttgggaaaac aacttctatc ggaaaactag cccaccgcta caaacgagct ggtaagaagg
    20941 tcatgctggt tgcagcagat accttccgtg cgggtgcagt agctcagcta gctgaatggg
    21001 gccgacgagt agatgttcca gtagtaactg gacctgaaaa agctgatcca gccagcgtgg
    21061 tctttgatgg tatggaacgt gccgtggctg aaggtatcga tattctcatg attgatactg
    21121 ctggtcgtct gcaaaataag gataacctta tggctgagtt ggaaaagatt ggtcgtatta
    21181 tcaaacgtgt tgtgccagaa gcaccacatg aaaccttctt ggcacttgat gcatcaacag
    21241 gtcaaaatgc cctagtacag gccaaagaat tttcgaaaat cacaccttta acgggaattg
    21301 ttttgactaa gattgatgga actgctcgag gaggtgtggt tctagccatt cgtgaagaac
    21361 tcaatattcc tgtaaaattg attggttttg gtgaaaaaat cgatgatatt ggagagttta
    21421 actcagaaaa ctttatgaaa ggtctcttgg aaggtttaat ctaatcagaa gcaaaaatcc
    21481 tgcaaggcat aaacttgcag gaaatttttt tattctaagc gaccatcttg acgataggtg
    21541 atatctggtt gccaagtcca tttggcaccg aatttttcaa gtaggtcaaa gctggcttga
    21601 ggtcccatgc ttccagcttt atagtcatga agtggggcac cattttcagc ccagagcttt
    21661 tcaatacggt caatcaactt ccatgacgca caaacttcat cccagtggct aaagttagtt
    21721 gagttgttat ttaggacatc ataaatcaat ttttcgtatg gttctggaga agcaccagtt
    21781 gcagtcgcat ctgtacggta atcaagtgag ttaggagcca agttaaattc ttctcctact
    21841 tgcttcccat ttaggctaag agagaagcct tctgttggtt gaatatagat ggtcaaaata
    21901 tttggagcaa gtggttctcc aaagatagaa tccatttgtt taaagacgat gttgacatga
    21961 gttccttttt cagtcagtcg tttacctgta cggaaaaaga aaggaacacc acggaatcga
    22021 tcgctgtcta caaagaaggc accagatgta aaggtttcag ttgttgattc tggattcaca
    22081 tttggctcgc tacgataaga gatgtatttc atgccatcaa tcttaccaga gcggtattgc
    22141 ccacggataa agtgttcttt gagttcttca tcagttggat gatagaggtt tttaaagacc
    22201 ttaatctttt cagcacgaat ctcgtctttt gtgaagcttg ctggtttgtc catggcgagg
    22261 agcgaaagaa gttgtagagt gtggttttgg accatgtcac ggagggcacc ggattggtca
    22321 tagtagccac cacgttcttc tacacccaag cgctccgcaa aggtaatttg aacattgtcg
    22381 ataaaatcct tgttccaaac gttttcaaaa atcaagtttg caaagcgaac tgcaaagatg
    22441 ctttggatca tttccttacc aagataatgg tcgatacgga aaatttgttc ttcgtcaaat
    22501 gttgctagga gttcgtcatt caacttgttt gcagttgcgt aatctgtacc aaatggtttt
    22561 tcaacgatca agcgctcaaa acctttgcca tcgacaatgt tttcagattt gaggtgtttg
    22621 gcaatggttc caaagaactg aggtgccata gacaagaaga agagcttatt gtgttcagct
    22681 tggtattttt cattaagctc agcttgtaat tgacgcaaag caatataatg ttccgaatca
    22741 ttgacatcat ggctttgata gtagaagtgg ctagcaaatt cttgggcttg ctcggtacta
    22801 tctgccaaat caaggatgga ctcgacaact acagattcaa aatattcctt actccaaggt
    22861 ctacgggcag ttccaataac ggcaaagtgc ttggaaagat tgccggattg atatagtcta
    22921 aaaagggaag ggtagagctt gcgtttagcc aggtctccac tcgcaccgaa aattgtaaca
    22981 ataaccttag atgacatcta gctacctaat ttctattttt attcctagtc ttgctaggta
    23041 tgaggaaacc tcatttcata aacttaattc taacataatt ttccgaaaaa tcaaaaattc
    23101 caatacgagg tagaaaaact gcaaggaaat ccttacagtt tgagtttaga cgtttaagac
    23161 cttatctaag aactctttca gacgagggtg ttgtgggtta tcaaagattt ggtcaggtgt
    23221 tccgtcttca aggaactcgc catctgcagt aaagataacg cggttggcaa cctgacgagc
    23281 aaatcccatc tcatgggtta cgataatcat ggtcatacct tgctcagcca attccttcat
    23341 aacgttaagt acgtctccaa ccatctcagg gtcaagggca gaagttggtt catcgaagag
    23401 catgatgtct ggattcattg ctaggccacg agcgatggcc acacgttgtt tttgaccacc
    23461 tgataggcta tctggattgg cattagcttt atctgctagt ccaacctttt caagcaactc
    23521 cattcccaat tcctcagctt cttccttagt catcaacttg tgctcaatag gagcaaaggt
    23581 gatgttgtcc aatacagaca tatgagggaa gaggttgaag tgttggaata ccatgccgat
    23641 attttcacgg acgtggtcaa cattggttgt tttttcagtt aaatcatagc cgttcacagt
    23701 gatgtgaccg ctagtgactt cttctaaaag attgaggcta cggaggaaag ttgacttacc
    23761 agaacctgaa ggaccgatga tacaaacaac atctccttca tagaacttag tcgtaattcc
    23821 ttttaggact tcattttttc catagtgctt gtgtaaatca tttacatcaa tttttaattt
    23881 tgccattaac gaatcctctt ttctaagcgt ttcgctagtc tagtcaaaag tgtgataatt
    23941 acaagataga agatagcaag gattgcatac atcttgaaac tttggtagtt acgagcaatg
    24001 ataatcttac cagtttggaa gagttcaacc aaaccgatag cagatacgat agttgtatct
    24061 ttaagagcga taacgaattg gttgacaaag tttggcaaca tcaatttagt tgcttgtggc
    24121 aagataatct tacgcatggt ttttccataa gagataccca agcttcggct ggcttccatt
    24181 tggccaactg gaacggcctg aataccacca cgaacgattt cagcgatata agcagccgca
    24241 ttgagtgaga gggcaattgt tccagctaca aagtcgttaa ttgggctttg ttggcctgtg
    24301 atagactcga tgaagtttgg aattccccag aagatgaagg ctgcaagaat catcaatgga
    24361 ataccacgaa taacgtcaac gaaaatctca gagatgacgc gaagagattt gtatgggcta
    24421 acgctaaaca taccgaagat aattccgatg acaatggcaa tagcaaatga gataagagct
    24481 agagcaagag tgataccaag accgctaagg agttgtttgt agttgttttg aagcaagccc
    24541 cagagcgttg tttcgtcaac agtacttgtt gaagcagttg aagattcgct agctaggtat
    24601 ttgtcaagaa tcttttggaa ttcaccgttt gctttaaggt ttgcaagtcc gttgttgaac
    24661 atttcaatca gttctggatt tgctcctttt ttaacggcaa aggctgtttc accgattgga
    24721 gttccagaga ttggagtttt caatttttga ccttggctga tagaatattt gagaacaggt
    24781 tcatcatcca taacggcatc aatggcacca gtgtttaaac tgtcatacat tgaagaacca
    24841 tcagcaaagg ttttgatttt gtaaccgtat ttgctttgat tttctgttag gaaggtttga
    24901 gaagcagttc cgtttttaac accgactgtc tttcctttta gatcttcata agaagcaata
    24961 ttgcttgatt ctttgacacc aagaatggta ttagcagtgt agtatgattc tgagaagtca
    25021 aaagttgcct tacgagcatc tgtgacagac ataccagcaa tgataccatc ggcttgacca
    25081 gcttggacag cactgatagc agcatcaaaa ccagggttgg tgatttcaat ttcaaaacct
    25141 tggtctttag cgattgcctt aatcaattcc atatcaatac cagtgtattg gttgcttgaa
    25201 ttttggaaaa caaaaggtgc aaaagaagaa tcgctggcaa tgatatattt agccttaaca
    25261 ggaatagctt ttaatcctgc gagagtagtt gtagttggca ctgctgaaga tgatgaagca
    25321 gtccatttct tgataatttt atcaagacta ccatcttttt tcatttcagc caaggcttgg
    25381 ttaaattcag taaccaggtg ctcgtattta cttccttttt tcacaccgaa agcaaaactt
    25441 cctacagctt caccatccat ttcaatatgg aggtcttgac cttggttaat ggcatattcg
    25501 ataacaggtt tgtcatccat catggcatcg atggcaccag cactcaagct gttgttcatt
    25561 aaatccccag tgtcaaatgt tttaatagta aagccgtatt tatctttgat tgtttcaagg
    25621 aaacgttgag cggcagttcc gtttttaaca ccaacggttt tgccagttaa ttggtcgtac
    25681 ttgctaattt tgtgtgactt tgtagtagca atgacaactt ttgtatcata gtaagtatca
    25741 gacatggtga agactttttc acgttcttta gtctttgtca tccctgccat gatagcgtcg
    25801 gcttgcccag cttgaaccgc attgactgct gcgtcaaatc caggatagga catctgaatg
    25861 ttccagcctt taatctcagc gactttgtta ataatgtcaa catcaattcc tttataagtt
    25921 tgatctgaat ctttaaactc aaaaggtgca taggcggtat cagaaacaat cttaatcgtt
    25981 tctgctttgg caatacctaa tgagaaaatt gggaataaaa ttagcaaaaa tgctagaaat
    26041 tttttcttca tttttagtct ccttttccga atattctctc attatatcag aaaaagtaaa
    26101 aaaaggcaaa tttttatatt tttcgtgtca gaaaatataa cattggtttt tccttttctt
    26161 tcttgaattg ctattaaaaa gtgctataat aatagtatat atagaagaaa agaggacggg
    26221 gatatgaaga acaaaagaat atttaaagac ttccaagctt caaaaatgag tttaaacatt
    26281 tacacaagcc ccttgttagc ctttgttttt gtcttcatag gagagtttgt ggcttatact
    26341 ttatatggta ttagtttgtt agctctcatc ggacttgcta gaaattttgg agaggctggt
    26401 caaaatcttg caagctactt gcagaccttg catcagagct tgacggataa aacaagtgac
    26461 tttcgtttaa ttttaggatt actggccttt ggttttattc ttaacactgt gttcagatgg
    26521 acaagaaaag ttgagaaaag acctattcga accttgggat tttatagaga gaatttcctc
    26581 agcaatcttc tgaaaggatt tagtctaggc ctggcacttt ttcttctgac cttgttaggt
    26641 ttggtggtct taggtcaata tcgtttggaa tccattcact tgaatcctta ttctcttgcc
    26701 tttgtcgtct ttactatccc attttggatt ttacagggga cagcagaaga agtggtggcc
    26761 cgtgcttggc tacttcctca attggcctca agaaccaatc taaaactagc tattcttata
    26821 tctagcctgt tctttaccct gcttcatatg ggcaattctg gcctcacccc tctatctcta
    26881 gtaaatctct ttttattcgg agttgccatg gctctttacc ttctcaaaac tgatacagtt
    26941 tggggtgttg caggtattca tggtgcttgg aattttgctc agggtaatct ctttgggatt
    27001 ttagttagtg gtcaaccgtc aggaacgtct ctgatgacct ttttaccaca aggcaatcaa
    27061 gattggctat caggtggttc ttttggcata gaaggttcca ttatgacaag tctggtatta
    27121 ctactgctga ttgtctatct tgctaataaa ttaaagaaag aaaatgaaag gatgtgactt
    27181 cggtccgtcc ttttcttcgt gaaaatacta taagtatgct aaaataggaa tagcacatgg
    27241 agagaggatt cttatgatca atcacattac agataatcaa tttaaactag tatcaaaata
    27301 tcaaccatca ggagatcaac cccaagctat cgagcagttg gtggataaca ttgagggggg
    27361 agaaaaagct cagattctga tgggggcgac tggaacaggg aagacctata ctatgagtca
    27421 ggtcatttct aaagtcaata aaccaactct ggttattgcc cacaataaaa ctctggctgg
    27481 tcagctctat ggggagttta aggaattttt ccctgaaaat gcagttgagt atttcgtatc
    27541 ctactatgat tattaccagc cagaggccta tgtcccttct agcgatacct atattgagaa
    27601 ggatagttct gtcaatgacg agattgacaa gcttcgccac tcagctacct cagcactttt
    27661 ggagcgtaat gatgttattg tcgtggcctc agtctcttgt atctatggtt tgggttcgcc
    27721 caaggaatac gctgatagtg tcgttagtct ccgtcctggt ctagagattt ctcgtgataa
    27781 actcttgaat gacttggtcg atattcagtt tgaacgcaat gatattgatt tccaacgcgg
    27841 aagatttcgc gttcgtgggg atgtggtaga gattttccca gcttcccgag atgaacatgc
    27901 ctttcgagta gaattttttg gagacgaaat tgaccgtatt cgtgaagttg aggctctgac
    27961 aggtcaggtg ttgggagaag tggatcattt agcgattttc ccagcgacac actttgtgac
    28021 caatgacgac catatggaag ttgccattgc aaagattcag gccgagttgg aagaacaatt
    28081 agctgtcttt gaaaaggaag gtaaactgct tgaagcccag cgtttgaaac agcggacaga
    28141 gtatgatatc gaaatgttgc gtgagatggg ctataccaat ggggttgaga actattcacg
    28201 acacatggat ggacggagcg aaggagagcc tccttatacg ctcttagatt tcttcccaga
    28261 tgatttctta attatgattg acgagagtca catgaccatg gggcaaatca agggcatgta
    28321 caatggagac cgttcgcgta aagaaatgct ggttaattat ggcttccgat tgccgtctgc
    28381 tttggacaat cgtcctctcc gtcgggagga gtttgagagt cacgttcatc agattgttta
    28441 cgtttcagcg acacctggtg actatgaaaa tgaacagact gagacagtga ttgagcaaat
    28501 cattcgtcca acgggactct tggatcctga agtggaagtc cgtccgacta tgggacagat
    28561 tgatgacctc ttgggtgaaa tcaatgcccg cattgaaaaa aatgagcgta cctttatcac
    28621 aactttgacc aagaaaatgg cagaggactt gaccgactac ttcaaggaaa tgggtatcaa
    28681 ggtcaagtac atgcactcgg atatcaagac cttggaacgg acggagatta tccgtgacct
    28741 gcgcttgggt gtctttgatg tcttggtcgg aattaacctg ctccgtgaag gaattgacgt
    28801 tcctgaagtg agcctcgtag ctattctcga tgctgataag gaaggtttcc ttcgcaacga
    28861 acgtggactc atccagacta ttggacgtgc tgctcgtaac agtgaaggcc atgtcatcat
    28921 gtatgcagac acggttaccc agtctatgca acgtgctatc gatgaaactg cccgccgtcg
    28981 gaaaattcag atgacctata atgaagagca tgatatcgta ccacagacaa tcaagaaaga
    29041 aatccgtgac ctgattgccg tgaccaaggc agttgctaag gaagaggaca aggaagtcga
    29101 catcaatagc ctcaacaaac aagagcgcaa ggaactcgtc aagaaactag aaaaacaaat
    29161 gcaagaagcc gtcgaagtgc ttgactttga actggcggct cagattcgtg atatgatgtt
    29221 ggaagtcaag gtcttggatt aggggaatag tatgatttat ttaagaaagt taaagagaga
    29281 agatttgatg tctttatggg aaatggctta ttcacaactt aatccagttt ggaaacagta
    29341 tgatgctccc tattatgatg attatcagta tttttcaaat tttaaagaat tcgaactaca
    29401 aaaatcagaa tccattttaa gcaactcaaa tcgccttggt atttttgttg atgataaact
    29461 agttgggact gtttcgcgtt attgggtatg taaacaaaca agatggatgg aattgggaat
    29521 tggtatttat gataaaaaat tctggaacac tggtattggg aaagttgcta tgttgcagtg
    29581 gatagatagg acgtttcagg attacttgga gttggagcat ctgggtttga caacttggtc
    29641 agggaatctt ggtatgatga aacttgctga aaaattaaga atgaaaaaag aagctcatat
    29701 tccaaaagtt cgttattatc aaggtaaata ttttgatagt attaaatatg gtattttgag
    29761 agaagactgg gagaaaataa atgacggtta ttatcaaatc aatggaaact cctgaagaga
    29821 tagaaggtaa atcccttgtt cactggcaaa cgtggagaga ggcttatgat gaccttttgc
    29881 ctgcagaatt tcaggagaca atgacattag aaagatgtcg attctttagt caaaagtatc
    29941 cagaaaatac attgattgcg ataggtgatc tgaaggtagt tggttttatc agttatggta
    30001 actttcgtga tgagactatt caagctggtg aaattattgc tttatatgtt ttaaaagact
    30061 attacggaaa aggcatagca caaaagttaa tgaaagcagc tttgactgct ctgaatcatt
    30121 tttctgaaat tttcttatgg gtattgaaag ataacaagcg agccattgct ttctatcaaa
    30181 aaatgggttt tacttttgat ggacaagaaa aaatacttga acttggaaag cctataaagg
    30241 aaaaacgaat ggtgttctat tctaaataat tttaaaaaag taaaagataa tatctctggt
    30301 agtaagtctg aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg ttcccaatta
    30361 acaattttaa caaatatctg tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag
    30421 tatcgcgccc ctgaaaacaa tcgctggtct ggttatgcct ttcctggagg tcatgtagaa
    30481 aatgatgagg cttttgcgga gtctgtcatt cgtgaaatct acgaagaaac agggttgact
    30541 atccaaaatc ctcaacttgt cggcattaaa aattggccac tagatacagg tgggcgctat
    30601 attgtcattt gttataaggc gactgagttc tctggtaccc ttcaatcttc agaagaggga
    30661 gaagtttctt gggtgcaaaa agaccagatt ccaaacttaa atctggccta tgatatgcta
    30721 ccattgatgg aaatgatgga agctcccgac aagtcagagt ttttctatcc tcgccgtaca
    30781 gaagacgatt gggaaaagaa aatcttctag tcttttacta aataacctag ctgatccaag
    30841 gcctcctcga tatagtggag gtcttgttgt gtttcggctt cgactaggtg gtaatggatg
    30901 ccatctgtca attcagatag aggtctaaag tcagaatgct cgacttgttc tagaaaatgt
    30961 tgcacatctc tgcgacaaga cagtttaagc aaggtttcaa tttctccata aacgggatgg
    31021 tcaatcaagg tattctggac atgcccacca ttatcgacaa tagcaaggag ttcctgaccg
    31081 atttcttcaa cttcatgttt cactttgaaa agtttgtgaa cataaggatt ggtatcaatt
    31141 tgcttataga tgtagccacg attggtggat aggataggag cgccatcggc tctcaaaatt
    31201 gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat gttccgccaa actttggcca
    31261 ttaagggctt taggagcttc tttcaagagt tggagcaggg cttgtttgcg atcttttgtc
    31321 atagcttttc cttttaacgg cgttttcgaa gcactttata gacagctagt gctaatgtat
    31381 agtctaccat actatggata attgtgccaa atccaactag tacaaataga acatagaaca
    31441 tattttctac attggtaccc gaagttgcgt aaaaaaggac acaggctaat acttcagcaa
    31501 gggcatgaac aacagccaaa acaaaattga aaacccagga agattttggt ttatctaggg
    31561 tatcggggaa tttttgtagg taaagagctc ctaaagcacc aaaagatata tgggaaaaag
    31621 cccgaaaaac gataaccatg ggatagccag ccatcaaaaa tccaaaacta gaggctagga
    31681 tgacaaaaac tgccatcaag ggcgacaaga acatggctat aaaaatagcg atgtggctcc
    31741 ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa
    31801 tcgcaatagc cgttaaaagg gctgtcattg tcataaattg tgtctttttc cgtgtattca
    31861 caagaatctc ctttttaact gcatatacac tagtatggta caataaacca gacaataaag
    31921 caagaattta cttgggttta tagatcattt tttagttaaa agttatagta gatggaaact
    31981 agaatagtcc acctctactt ctaaaacatt gttagcgatt tgactgtcct gatcgatttg
    32041 tcctgttctt atttcgtttt actatagtaa agatttcatt aaaaagaaac tgtatagagc
    32101 aaaatctcca ccttcaggtt tgaaagcgga gattgttttt attttttcaa ggtttgtagt
    32161 cgtggaacaa tggctagggt tagaattgca gaaatgacca attcagcaat tgaatttgtt
    32221 gagataacgg ttgccagaag aagttggata tttccattat aaacatttcc aaataggaag
    32281 aagattcctc caaggacaaa gatagtattt gtcaaggaac caagggctcc agctaaaatc
    32341 agaccagtct tgtttttcat cagtttatag actaagtaag gagttaaacc aatcaaaata
    32401 cgtgggacga tggcaatgat agctgagtag atgtttccgt ttggtacgaa gggagagaag
    32461 aggtagcttg tcggtagaat cgtaatcgtg ttaaccgtca agctaagtaa tcccatcaaa
    32521 aatccaagtg taaccccaac tcgtggacca taaataatgc tggcaataat gacaggaata
    32581 tgaacaatgg tcggtttgat tggaaatgga aaaaggttaa agataagtga gctcagaaag
    32641 tgtatcacga gcatggtagc aaaaaagata gcaatgggtg caatattaga gcgttttttc
    32701 atcgatagtt tcctttattc tttctaaaat aattgtgagg tcagctaaag ctcctcgtcc
    32761 gtggtctcca caagctagta gggattcctt aggagcaatc agctgatagc cgtaggtttc
    32821 taatgttttc agattattct gagttactgg atggtcatac atttttgtat tcatagcagg
    32881 agctattagt ttgggaatat gacttggtag ggctagagct gtactggtta ccatgttgtc
    32941 cgcaaatccg tgagctagtt ttgcaatagt gttagcagtt gcaggtacca cgataaataa
    33001 atctgctttt tttccaagtt cgatatgatt gacctgatca ggatagggtt ccttcatgac
    33061 atccaagtgg acaggattct gtgagagtac ctgtagtgtc aaaggttgga taaactctgt
    33121 agcagcctga gtcattaaga cagtgacttg atggccttgt ttttttagag aactgactaa
    33181 atctgccgac ttataagagg cgattgaacc cgttacagcc aagagaatgt ttgccatagt
    33241 tttcctttct aagaatgata ggcttgaatt ttttcaagga ggagttctgc aatttcttct
    33301 ttagtctgga ctgtttgaag ctgatttttc tcaacaaata tagctcggtg ctgatctgct
    33361 gaaatttgag tcaggtcatt cgcgatgatt aaatctgctt gattcttgat aagacttttt
    33421 cgtgcaatgt caaccagatg atcttcggta acatcaacca gcagtttgaa accaatcaga
    33481 tgaatagtgg gattccattc cttgactagg gatatgattt tgggtgtctt tttaaggaac
    33541 aaaacctgaa cctcatcagt tgaagaaatc ttggcctgat gattctgctt gcttaaaaat
    33601 tcttttagat tggagctagc ctgaacttcc tcaagccctg tcatataaac aggagtgtag
    33661 tcagaaacag ccattgagtg gatcaagacc tgataatcct gaacacgttc ttgcatttct
    33721 attagaaggt ccttggtatt ggtaatttct cgaatactta ggttaggatg aggctctggc
    33781 ttcagagctc gttttgtcgt aattaaacaa acttcatgcc ctgcagaaag caaagtctct
    33841 gtgataattt tccccaagtg acctgtagaa tggttagtga tagagcggac gctatcgata
    33901 gcttcactgg taccgcccga tgtaactaaa attttcatag cactattgta cacaaaaata
    33961 agcggtaagt aaaatgaaag tgataaattt ttggtaaaaa cgaagattta ctatagtttc
    34021 aaactataaa gccatataaa atttaagaaa ggactctaaa agccttaaaa acagagatat
    34081 ttacgaacgt ttagagagtt ttaggatgtt aaaaatcttg ttttgtgata taatgaatta
    34141 tcatataaga ggttagagaa attttgaatg aaaacagata ttgaaatcgc acagagtatt
    34201 gagttgaagc caattgttga tgttgtagag aaacttggta tttcttatga cgatttggag
    34261 ttgtacggaa agtacaaggc taaactcagc tttgataaaa ttcgtgcagt tgagagcaat
    34321 ccagtcggta aattgatctt ggttactgcc atcaacccaa cacctgcagg tgaaggaaag
    34381 tcgacgctta ccattggtct tgcggatgcc ttgaacaaga ttggcaagaa aaccatgatt
    34441 gctatccgcg aaccgtctct tggtccagtc atggggatca agggtggtgc tgctggtggt
    34501 gggtatgcac aagttctgcc aatggaagac atcaacctcc actttactgg agatatgcat
    34561 gctattacaa ctgccaacaa tgccctttct gccttgattg acaaccactt gcaccaaggg
    34621 aatgagctgg aaattgatca acgtcgtatc ctctggaaac gcgttgtgga cttgaacgac
    34681 cgtgcgctcc gccatgtgac tgttggtctt ggtggtcctc taaacggtat tccacgtgag
    34741 gatggttttg atattacagt tgcttcagaa atcatggcaa ttctttgctt ggcaacggac
    34801 atcgaggact tgaaacgtcg tttggcgaat atcgttattg gttatcgcta tgaccgtacg
    34861 cctgtttctg taggtgattt gcaggttgag ggtgccttgg ctttgatttt gaaggatgct
    34921 attaagccaa acttggttca gacaatttac ggtacacctg cctttgtaca cggtggtcca
    34981 tttgccaata tcgctcatgg ctgtaactct gttttggcga cgacaacagc ccttcacttg
    35041 gctgattaca ctgttactga agctggtttt ggtgcggacc ttggtgctga gaaattcctt
    35101 gatatcaaga caccaaactt gccaacatct ccagatgcag ttgtcattgt cgcaaccctt
    35161 cgtgccctta agatgaatgg tggtgtggct aaagacgctc tgactgaaga aaatgtagag
    35221 gcagttcgtg caggttttgc taacttgaaa cgccacgttg aaaatatccg taagttcggt
    35281 attccagcag ttgtagctat taacgaattt gtatctgata cagaagctga aattgcagtc
    35341 ttaaaagaac tctgtgcctc aatcgatgta ccagttgaat tggctagtgt ctgggctgat
    35401 ggagcagaag gtggagtagc acttgccgaa acagttgtta agacaattgc tgaaaatcca
    35461 gctaactata aacgtttgta cgataatgcc ctttctgtcc aagaaaagat tgaaaaaatt
    35521 gtcactgaaa tctatcgtgg tagcaaagtg aactttgaga agaaagctca aacacaaatt
    35581 gctcaaatcg ttcaaaacgg ttgggacaaa ttgccaatct gtatggctaa aactcaatac
    35641 agtttctcag acaatccaaa tgcgcttgga gcaccagaaa actttgaaat taccattcgt
    35701 gaattggtac caaaattagg tgcaggcttc atcgttgcct taactggtga tgttatgacc
    35761 atgccaggtc ttccaaaacg tccagcagct ctcaacatgg atgttgaaag cgatggaact
    35821 gtactaggct tgttctagtg gattgaaatt gactttaagt gagtttggaa ataatatcca
    35881 agctcatttt ttctcagact gcaagcagtt catcaataag atttatactc ttcgaaaatc
    35941 tcttcaaact acgtcagctt caccttgccg taggtatatg atactgactc tgtcagttct
    36001 atccacaacc tcaaaacagt gttttgagca acctgcggct agcttcctag tttgctcttt
    36061 gattttcatt gagtataagt agatagaaaa gttatatact atctattgaa aatattataa
    36121 atcagataca aagacaggtg aaaccttctg tgttatacta gagatatgtt agatttgaaa
    36181 gaatacggta tcgtcatgtg gccggaggag aaagtcattt ctttccgtga gaaacttctc
    36241 gcttggtatg atgaaaacaa aagagatttg ccttggagga gaagtaaaaa tccttatcac
    36301 atctgggtat ctgaaatcat gcttcagcag accagggtgg atacagttat cccttactac
    36361 gaaagattcc tggactggtt tccaactgtc gaaagtctgg caactgcgcc tgaggagagt
    36421 ttactgaaag cttgggaggg cttgggctat tattctcgag ttcgcaatat gcaggctgca
    36481 gcccagcaga ttatgactga ctttggtggc caatttccaa atacctatga aggaatttcc
    36541 agcttgaaag ggattggacc ttacacagca ggagccattt ccagtattgc ttttaacttg
    36601 cctgagccag ctgtagatgg taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac
    36661 cacgatattg ggattccaag taatcgaaaa atttttcagg caatgatgga gatcttgatt
    36721 aatccggatc gtccgggtga ttttaatcaa gccttgatgg acttagggtc tgatattgag
    36781 tctcctgtaa atcccagacc tgaagaaagc ccagtcaagg actttagtgc ggcatatcag
    36841 aatggaacca tggaccgtta tccaattaaa tctcctaaga aaaagccggt tcctatttat
    36901 cttaaagcat tagttgtgaa aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc
    36961 gaaaagctct tggcaggttt ttggcatttc ccccttatag aagttgataa cttttcgcaa
    37021 gaagagcagt ttgacctctt tcatcaggtt gcggaagaaa gtgtgaactt tggccccagt
    37081 ccagaagaga gtttccagca ggactatgac ctagatgttg attggcttga tgtttgtttt
    37141 gatacagtcc agcatgtttt tagtcatcga aaatggcatg tacagattgt agcaggtcag
    37201 gtgagtgact tccatgattt ttcagatagg gaagttcgtt ggctttcacc agaagaattc
    37261 aagaattacc cacttgccaa accccaacaa aaaatctggc aggcttatgc acaagccaac
    37321 ttagacagta gccaagacta gctattgtgt cttctgacta ttttttggta taatagtaga
    37381 gaaaaaggtg aacatatgaa aaaaatacta attgtagatg atgagaaacc aatctcggat
    37441 attatcaagt ttaatatgac caaggaaggt tacgaagttg taactgcttt taatggtcgt
    37501 gaagcgctag agcaatttga agcagagcaa ccagatatta ttattctgga tttgatgctt
    37561 ccagaaattg atggtttaga agttgctaag accattcgta agacaagcag tgtgcccatt
    37621 cttatgcttt cagccaaaga tagtgaattt gataaggtta tcggtttgga acttggggca
    37681 gatgactatg taacgaaacc cttctccaat cgtgagttgc aggcgcgtgt taaagctctt
    37741 ctgcgtcgtt ctcaacctat gccagtagat ggtcaggaag cagatagtaa acctcaacct
    37801 atccaaattg gggatttaga aattgttcca gacgcctacg tggctaaaaa atatggcgaa
    37861 gaactagact taacccatcg tgaatttgag cttttgtatc atttagcatc gcatacaggt
    37921 caagtcatca cgcgcgaaca cttgcttgag actgtctggg gttatgacta ttttggtgat
    37981 gtccgtacag ttgatgtgac tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc
    38041 cgaccagagt atatcttgac gcgccgtggt gtagggtatt acatgagaaa taatgcttga
    38101 tttactgaaa caaaccattt ttaccagaga ttttatcttt atcctgattt tgttaggttt
    38161 catccttgtt gtgaccctct tattactgga aaatagacgt gataatattc agttgaagca
    38221 aatcaatcaa aaggttaaag atttgattgc aggagattat tccaaggttc ttgatatgca
    38281 aggtgggtct gaaatcacca atattaccaa taatttgaat gacttgtcgg aggttattcg
    38341 tctcactcag gaaaatctag aacaagagag taagaggcta aatagtattc tgttttatat
    38401 gacagatggg gttcttgcga ccaaccgtcg gggtcagatt atcatgatta acgatacagc
    38461 caagaagcaa ctggggttgg ttaaggaaga tgttctgaat agaagcattt tggaattgct
    38521 caagatagaa gaaaactatg aattgcgtga tttgattacc caaagtccag aattgttgct
    38581 agattcccaa gatatcaatg gcgaatattt gaaccttcga gttcgctttg ccttgatacg
    38641 tcgagagtct ggctttattt caggtttggt ggctgttttg catgatacga cggagcagga
    38701 gaaggaagaa cgcgaacgaa gactctttgt ttccaatgtt agccatgagt tacggactcc
    38761 tctgactagc gtaaaatcct atcttgaagc cttggatgag ggggctttgt gtgaaactgt
    38821 agcaccagac tttatcaagg tttctcttga tgagaccaac cgtatgatgc gcatggtgac
    38881 ggatctcctc catctttcac gtattgataa tgctaccagt cacctagatg tggaactgat
    38941 taacttcact gcttttatta cctttatcct caatcgtttt gacaagatga aaggacagga
    39001 aaaggagaaa aaatatgagt tggtgagaga ttatcccatc aattctatct ggatggaaat
    39061 tgatacagat aagatgacgc aggttgtcga caatatttta aataatgcta ttaagtattc
    39121 gccagatggg ggtaaaatca ctgtcagaat gaagacaact gaagaccaga tgattttatc
    39181 catttctgac cacggtttgg ggattcctaa gcaggattta ccacgtatct ttgaccgttt
    39241 ctatcgtgtg gatcgtgcta gaagtcgtgc acaaggtggt acaggtctag gactgtctat
    39301 cgctaaagaa attatcaaac aacataaggg ctttatttgg gccaagagtg aatacggcaa
    39361 gggttcaacc tttaccattg tgctccctta tgataaggat gcagtgaaag aagaagtatg
    39421 ggaggatgaa gtagaagact agaatgagtg aaataggctt taaatacagt attttagcgt
    39481 cgggttccag tggaaattct ttttatctgg aaacctcaaa aaagaagctt ttagtagatg
    39541 caggcttgtc tggcaagaaa attaccagtc tgctagctga aattaaccgt aagccagaag
    39601 acctggatgc catcttgatt acccatgagc attcagatca tatccatgga gtaggcgttt
    39661 tggctcgcaa gtatggtatg gatctttatg ccaatgaaaa gacctggcaa gctatggaaa
    39721 atagtaaata tcttggcaag gtggattctt cgcaaaagca tatttttgaa atgggtaaaa
    39781 ccaaaacctt tggagatatc gacatcgaga gttttggtgt aagccatgat gcagtcgcac
    39841 cgcagttcta tcgctttatg aaggatgata agagttttgt cctcttgaca gatacaggtt
    39901 atgtcagtga ccgtatggcg ggcattgtcg aaaatgcgga tggttatctt atcgaggcca
    39961 accatgatgt agagattttg cgatcaggtt cttacgcttg gcgcctcaaa caacgaatcc
    40021 tatctgacct tggtcacctt tctaacgagg acggtgctga agctatgatt cggacgctag
    40081 gaaatcgtac taagaagatt taccttgggc atttatctaa ggaaaacaat atcaaggaac
    40141 tggctcatat gaccatggtc aatcagctgg ctcaagctga tctgggagtc ggagtagact
    40201 ttaaggttta tgatacctca ccagataccg caacaccatt gacagagata tagaaagaac
    40261 gctgagaagg tgttcttttt atattgactg gacacctaaa aagtaataca atggtgttac
    40321 cattaaaaaa gggagcacaa aagatgacta ctattacatt aaaagtttct gaagctgata
    40381 aaacatttat gaaagcaatg gctaagtttg aaggagtttc cctttcggaa cttattcgca
    40441 ccaaaactct tgaagcccta gaagatgaat acgatgctcg tgtggcagat ttagcctatc
    40501 aagagtattt agaagacttg gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc
    40561 atgatttagg cttgaaggat gaataatttg tataaattag ttccaacaag acgttttatc
    40621 aagcaattga aaaaattgga ccgttatacg cagaagctaa ttacaaacta tttacaaacc
    40681 aatgttttgg aagatccaag acgacacgga aaggctttgg ttggtaatcg cgttggtcaa
    40741 tggcgctata gaattggtaa ttatcgagtt atcgtacaaa ttgtagatga tgaattagtt
    40801 gttgctactc tagaagttgg tcatcggaga gatatttatt aaattaaagc tgagtaataa
    40861 cagtatagaa agtaaggaga gtaaaaattt gaagaaaaaa tggttttttg cagattatta
    40921 tgacacaaca attattttac ttgctttaat ctctgttatt ctggtattac ttggatttgc
    40981 tgagatgatt gatttggaca atccgcctta tagtattatt gatttagtaa tctggggtgt
    41041 ttttgtaatt gattatagtt ggcgcttttt tacgactaaa agaaaatggc gctttattct
    41101 tgaaaatatt tttgatttat tggctattct ccctttaaat gctattttta cagtgtttcg
    41161 attagggcgt atctttcgtt tagctaaact aacaaaatta ctgaaactga ctcgtttact
    41221 tagaataatt ggacttacag gtaaattaga aagaaaaatt agtagattct tacggacaaa
    41281 tggcctgatt tatatcttgt atgttaatat ctttattgtt ctagtaggga gttcgatttt
    41341 atccgttgtt gaagaaaaat ccttctcaga tagtctttgg tgggctcttg tgacagtaac
    41401 tactgttgga tatggggata ttgttcctgc ttccattttt gggaaatggc tagcagtttt
    41461 actgatgctt gtcggtattg gaacaatagg gatgttaaca agtgctttga cgaacttttt
    41521 tgtaaaggat aatccagatg aacagataaa acttgataaa ctcaaagatg aattatcgag
    41581 ccaaagaata ttactagaga aacaatctaa aaagatagaa gaactacata aaatgataca
    41641 agatttgatt gaaaaaacat aataaaaata aagatgagac aaagtttgag ctgaggagaa
    41701 atcaagtaag ttttgacaca atagaacgca tattatcagg tataaaaact tgatactatg
    41761 cgttttatag agaaataaaa taaaatctga acaaatcaat taggaattta aagcatttta
    41821 taacaacgtt ttagagtgat ggggtgctat ttcaacttca acctactata atacagaaaa
    41881 aaacaactcc ctgatgattc aaggagttgt ctatagttaa attagttttt agaagcttct
    41941 tggaattctg gatttttcca tgcttcgtca ataatagctt gcaattcttt agcagatgct
    42001 tgcatttttt gagtttctgc atcgttcaat ggaatgttta ctggacgaac gataccatgt
    42061 gcaccaacaa cagctggttg accgataaag acattctcaa ctccgtatag accttcttgg
    42121 aatactgaaa gtggaagtac tgcgttttca tcgtcaagga ttgctttagt gatacgagca
    42181 agggctactg cgataccgta gtatgttgca ccttttttgt tgatgattgt gtaggctgca
    42241 tcacgaacac cttcgaacaa ttcaatcaat tcagcttctt gaacattttg agtgtcttta
    42301 aggaattctt caaggtttac accagcgatg ttagcgtgtg accaaacagc gaactcagag
    42361 tcaccgtgtt cacccatgat gtaggcgtgc actgaacgag catccacatc caatttttca
    42421 gcaagtgctt gacggaaacg agctgagtca agtgaagtac ctgaaccgat aacgcgttct
    42481 ttagggaaac cagagaattt ccaagttgag taagtcaaaa cgtcaactgg gttagcagca
    42541 acaaggaaga tacctttgaa accagattca acaacttgag ttacgattga tttgttgata
    42601 gcaaggtttt tacctacaag gtcaagacga gtttcacctg gtttttgagg tgcacctgca
    42661 gtaatcacaa caaggtcagc gtctgcacag tcagagtatt gagctgcata gattttttta
    42721 ggtgaagtga aggcaagggc gtgactaagg tcaagcgcat caccaacagc tttttcatgc
    42781 aattgtggaa tttcgataat tccaagctct tgtgcaattc cttggttaac aagtgcaaaa
    42841 gcgtaagatg aacctacagc accatcaccg acaaggataa cttttttgtg ttgtttagtt
    42901 gaagtcattg ttttaaacat ctccttaatt ttattagggg attttcccta gacaacttca
    42961 ttctatcact tttaaaaaac tttgtcacga atatgcctta tagttctcga tgtaaacgtt
    43021 ttagtggttt agaggctgaa atagatggga atttatggta taatgttgtt acttactaat
    43081 tgtgaaatga ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag
    43141 gagatgaagg caagttttat cgactacgcc atgagtgtta tcgtagcgcg agctcttcct
    43201 gatgttcgag atggcttaaa acctgttcac cgtcgcattc tctacggaat gaatgaattg
    43261 ggtgtgaccc cagacaaacc ccataaaaaa tctgctcgta ttacagggga tgtcatgggt
    43321 aaatatcacc cacacgggga ttcctctatt tatgaagcca tggtccgtat ggctcaatgg
    43381 tggagctacc gttacatgct tgtagatggt catgggaatt ttggttccat ggatggagat
    43441 agtgctgccg ctcaacgtta taccgaggca cgtatgagca agattgctct ggaaatgctt
    43501 cgtgatatca acaaaaatac agttgatttc gttgataact atgatgccaa tgaacgggaa
    43561 cccttggtct tgccagcgcg ttttccaaac cttttggtta atggagcaac tggtatcgcg
    43621 gttgggatgg caaccaatat tccacctcat aatctgggtg aaaccattga tgcagtgaag
    43681 ttggtcatgg ataatcctga agtgactacc aaggacttga tggaagtctt gcctggacca
    43741 gattttccaa ctggtgctct tgtcatgggg aaatcaggta tccataaggc ttatgaaaca
    43801 ggtaaaggtt cgattgccct acgttctcgt acagagattg aaacgactaa gactggtcgt
    43861 gagcgcatcg ttgtaacaga atttccttac atggtcaata aaaccaaggt gcatgagcat
    43921 attgttcgct tggttcagga aaaacgcatt gagggtatca cagcagtacg tgatgagtca
    43981 aaccgtgaag gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc caatgttatt
    44041 ctcaataacc tcttcaaaat gacccaaatg caaaccaatt ttggtttcaa tatgctcgct
    44101 atccaaaatg gtataccgaa aattttgtct cttcgtcaga ttttggatgc ttatatcgag
    44161 caccaaaaag aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc
    44221 gctcatatct tagaaggtct cttgattgcg ctagaccata tcgacgaagt gattcgtatc
    44281 atccgtgcta gtgaaacgga tgcggaagct caagctgagt tgatgagcaa gtttaagctt
    44341 tctgaacgtc aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac aggtttggaa
    44401 cgcgataaga ttcaatctga gtatgatgac ctcttggctc tgattgcgga tttagcagat
    44461 attcttgcta agcctgaacg tgtttctcaa attatcaaag acgaattgga tgaagttaaa
    44521 cgtaaatttt ctgataagcg ccgtacagag ttgatgattg gacaggtctt gagtctcgag
    44581 gatgaggact tgattgaaga atcggatgtc ttgattaccc tttctaacag aggctacatt
    44641 aagcgtttgg atcaggacga gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga
    44701 acgggagtga aagatgatga ctttgttcgt gagttagtgt caactagcac ccatgatcat
    44761 ctgctcttct tcacaaacaa gggacgtgtc tatcgtctta aaggttatga aattcctgag
    44821 tatggtcgga ctgccaaagg gctaccagta gtcaatctct tgaaattgga tgaagacgaa
    44881 agtattcaga cggttatcaa tgttgagtct gatcgcagtg atgatgctta tctcttcttt
    44941 acaacccgtc acggtattgt gaagagaacc agtgttaagg agtttgccaa tattcgtcaa
    45001 aatggtctca aagcgctgaa tttaaaggat gaagatgagt taatcaatgt cttgttggca
    45061 gaaggagata tggatattat cattggtacc aagtttggtt atgcagttcg ctttaatcaa
    45121 tcagccgttc gtggtatgag tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa
    45181 ggagacacag ttgttggtgc cagcttgatt actgatcaag atgaggttct tattatcaca
    45241 gaaaaaggat atggtaagcg tacagtcgct actgaatacc caacaaaagg tcgtggtggt
    45301 aagggaatgc agacagctaa aattaccgaa aaaaatggct tgctggccgg tcttatgact
    45361 gttcaagggg atgaggattt gatgattatc actgatacag gtgtcatgat tcgaaccaat
    45421 cttgccaata tttcacaaac aggacgtgca actatgggag ttaaagtaat gcgcctggat
    45481 caagatgctc agatagtgac tttcacaacg gttgcggtgg cagaaaaaga agaagttggg
    45541 acagaaaacg aaacagaagg tgaagaataa tgtctcaaaa aaataataaa aagaaaaaca
    45601 agcgaaaaaa tctgctgaca aatatcctag caggatttct gatattactg tcactggctt
    45661 tgatttttaa tactcaaatt cgaaatattt tcatagtctg gaataccaat aagtatcaag
    45721 ttagccaggt atcaaaagaa aaattagaag aaaatcagga tacagaaggc aattttgact
    45781 ttgattctgt caaagctatc tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa
    45841 aattaccagt tattggggga attgcaattc ctgaattgga aatgaatttg ccgattttta
    45901 aaggacttga taatgttaat ctcttctacg gagctggtac aatgaaacgc gagcaagtaa
    45961 tgggagaagg aaattatagt ctagctagtc accatatctt tggtgttgat aatgctaata
    46021 aaatgttatt ttctccttta gataatgcta aaaatggcat gaagatttat ctaaccgata
    46081 aaaataaagt ttatacttat gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg
    46141 atgaagttga tgatagagat ggggtcaatg aaatcacatt agtaacctgt gaagaccttg
    46201 ctgctacaga acgtattatt gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa
    46261 catctgatga aattctaaca gctttcaatc aaccatataa acaattttat taatacaaat
    46321 cagtgaaatc atggatttca ctgatttttt aagattttca taaaaatata gtaaaatgaa
    46381 ataagaatag gacgaatcg
//
LOCUS       DAVBCR010000007        46026 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCR010000007 DAVBCR010000000
VERSION     DAVBCR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.007"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQ508_000347"
     CDS             <1..128
                     /locus_tag="VQ508_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2489405.1"
                     /translation="FQSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(257..460)
                     /locus_tag="VQ508_000348"
     CDS             complement(257..460)
                     /locus_tag="VQ508_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489406.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(453..1535)
                     /locus_tag="VQ508_000349"
     CDS             complement(453..1535)
                     /locus_tag="VQ508_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2489407.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1661..2443)
                     /locus_tag="VQ508_000350"
     CDS             complement(1661..2443)
                     /locus_tag="VQ508_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2489408.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2443..3786)
                     /locus_tag="VQ508_000351"
     CDS             complement(2443..3786)
                     /locus_tag="VQ508_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2489409.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3885..5201)
                     /locus_tag="VQ508_000352"
     CDS             complement(3885..5201)
                     /locus_tag="VQ508_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2489410.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5394..6615
                     /locus_tag="VQ508_000353"
                     /pseudo
     CDS             5394..6615
                     /locus_tag="VQ508_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6814..8388
                     /locus_tag="VQ508_000354"
     CDS             6814..8388
                     /locus_tag="VQ508_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2489411.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8652..9440
                     /gene="codY"
                     /locus_tag="VQ508_000355"
     CDS             8652..9440
                     /gene="codY"
                     /locus_tag="VQ508_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2489412.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9440..10015
                     /locus_tag="VQ508_000356"
     CDS             9440..10015
                     /locus_tag="VQ508_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2489413.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10097..10182
                     /locus_tag="VQ508_000357"
     tRNA            10097..10182
                     /locus_tag="VQ508_000357"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10131..10133,aa:Leu,seq:aag)
     gene            10465..11595
                     /gene="ugpC"
                     /locus_tag="VQ508_000358"
     CDS             10465..11595
                     /gene="ugpC"
                     /locus_tag="VQ508_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2489414.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12096..12854
                     /locus_tag="VQ508_000359"
     CDS             12096..12854
                     /locus_tag="VQ508_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2489415.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12941..13453
                     /locus_tag="VQ508_000360"
     CDS             12941..13453
                     /locus_tag="VQ508_000360"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2489416.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13635..14579
                     /locus_tag="VQ508_000361"
     CDS             13635..14579
                     /locus_tag="VQ508_000361"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2489417.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14588..15265
                     /locus_tag="VQ508_000362"
     CDS             14588..15265
                     /locus_tag="VQ508_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2489418.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15363..16121
                     /locus_tag="VQ508_000363"
     CDS             15363..16121
                     /locus_tag="VQ508_000363"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2489419.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16159..17664
                     /gene="lytC"
                     /locus_tag="VQ508_000364"
     CDS             16159..17664
                     /gene="lytC"
                     /locus_tag="VQ508_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2489420.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18160..18678
                     /locus_tag="VQ508_000365"
     CDS             18160..18678
                     /locus_tag="VQ508_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2489421.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18808..19314
                     /locus_tag="VQ508_000366"
     CDS             18808..19314
                     /locus_tag="VQ508_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2489422.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNYAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19516..20748
                     /gene="clpX"
                     /locus_tag="VQ508_000367"
     CDS             19516..20748
                     /gene="clpX"
                     /locus_tag="VQ508_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2489423.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20757..21344
                     /locus_tag="VQ508_000368"
     CDS             20757..21344
                     /locus_tag="VQ508_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2489424.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21355..21735
                     /locus_tag="VQ508_000369"
     CDS             21355..21735
                     /locus_tag="VQ508_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2489425.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21787..22677
                     /gene="rapZ"
                     /locus_tag="VQ508_000370"
     CDS             21787..22677
                     /gene="rapZ"
                     /locus_tag="VQ508_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2489426.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22674..23651
                     /locus_tag="VQ508_000371"
     CDS             22674..23651
                     /locus_tag="VQ508_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2489427.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23648..24559
                     /gene="whiA"
                     /locus_tag="VQ508_000372"
     CDS             23648..24559
                     /gene="whiA"
                     /locus_tag="VQ508_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2489428.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24593..25561)
                     /locus_tag="VQ508_000373"
     CDS             complement(24593..25561)
                     /locus_tag="VQ508_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2489429.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25697..26554
                     /locus_tag="VQ508_000374"
     CDS             25697..26554
                     /locus_tag="VQ508_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2489430.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26541..27320
                     /locus_tag="VQ508_000375"
     CDS             26541..27320
                     /locus_tag="VQ508_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2489431.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27344..28696
                     /locus_tag="VQ508_000376"
     CDS             27344..28696
                     /locus_tag="VQ508_000376"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2489432.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28770..29144
                     /locus_tag="VQ508_000377"
     CDS             28770..29144
                     /locus_tag="VQ508_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2489433.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29146..29994
                     /locus_tag="VQ508_000378"
     CDS             29146..29994
                     /locus_tag="VQ508_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2489434.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30271..31038
                     /locus_tag="VQ508_000379"
     CDS             30271..31038
                     /locus_tag="VQ508_000379"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2489435.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31050..32234
                     /locus_tag="VQ508_000380"
     CDS             31050..32234
                     /locus_tag="VQ508_000380"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2489436.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32231..34102
                     /locus_tag="VQ508_000381"
     CDS             32231..34102
                     /locus_tag="VQ508_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2489437.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34245..35429)
                     /gene="mntE"
                     /locus_tag="VQ508_000382"
     CDS             complement(34245..35429)
                     /gene="mntE"
                     /locus_tag="VQ508_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2489438.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35733..38429
                     /locus_tag="VQ508_000383"
     CDS             35733..38429
                     /locus_tag="VQ508_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2489439.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38442..39233
                     /gene="yghU"
                     /locus_tag="VQ508_000384"
     CDS             38442..39233
                     /gene="yghU"
                     /locus_tag="VQ508_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2489440.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39349..39759
                     /locus_tag="VQ508_000385"
     CDS             39349..39759
                     /locus_tag="VQ508_000385"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2489441.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39771..41366
                     /locus_tag="VQ508_000386"
     CDS             39771..41366
                     /locus_tag="VQ508_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489442.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNIEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            41533..41697
                     /locus_tag="VQ508_000387"
     CDS             41533..41697
                     /locus_tag="VQ508_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2489443.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            41776..42504
                     /gene="yaaA"
                     /locus_tag="VQ508_000388"
     CDS             41776..42504
                     /gene="yaaA"
                     /locus_tag="VQ508_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2489444.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42555..44039)
                     /locus_tag="VQ508_000389"
     CDS             complement(42555..44039)
                     /locus_tag="VQ508_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2489445.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            44255..>46026
                     /locus_tag="VQ508_000390"
     CDS             44255..>46026
                     /locus_tag="VQ508_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2489446.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDG"
BASE COUNT        13703 a         8518 c        10394 g        13411 t
ORIGIN      
        1 cgtttcagtc gcttaatcaa ttggaacaag ctattgtaga ctatattgat tactacaaca
       61 ataaacgaat taaggtaaaa ctaaaaggac ttagtcctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actgtttggg ggcagtacat attcaacacc tgatactatg
      181 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
      241 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
      301 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
      361 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
      421 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
      481 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
      541 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
      601 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
      661 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
      721 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
      781 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
      841 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
      901 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
      961 aagaccatgg tatcaaagct catttcataa ccttcacgtt tcatggcaaa gtcgatttgt
     1021 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
     1081 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
     1141 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
     1201 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
     1261 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
     1321 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
     1381 ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
     1441 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
     1501 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tacccctcta
     1561 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
     1621 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
     1681 ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca taggcaggga
     1741 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
     1801 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
     1861 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
     1921 tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag taggtttcat
     1981 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
     2041 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
     2101 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
     2161 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
     2221 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
     2281 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
     2341 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
     2401 aggggtaatt gctatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
     2461 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
     2521 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
     2581 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
     2641 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gctcaaagtc
     2701 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
     2761 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
     2821 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
     2881 aaagacagca cggctcttgt caaatccctg tttgatgagt tgggaatctg cgccgaggaa
     2941 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
     3001 accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
     3061 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggcacc
     3121 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
     3181 cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa aaccaaaata
     3241 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
     3301 agtagcagtt ggaacttttc gaatggcatc caatatcatg ttataggtag tatagatttc
     3361 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
     3421 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tatttttccc
     3481 agtttttgaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
     3541 gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag tgagggcagt
     3601 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggct
     3661 ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac ttccacgtcc
     3721 aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
     3781 gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     3841 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     3901 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     3961 ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga accttctgag
     4021 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     4081 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4141 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4201 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4261 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
     4321 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
     4381 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4441 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4501 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
     4561 tcaatgccat cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4621 ggtaggaatg tatccaaggc atctaggact gtgaccttgc ttccaagttt gttgtaaagg
     4681 ccggcaaatt caagaccgat atttccgcca ccaaggattc caagtttttc aggtaatttg
     4741 tccaagcttt ggatacctgt tgagtcaaag atgtttttgc ttgtagcaag tccaggaatt
     4801 ggcaagacgt ttgaaacagc accagtgttg atgacgattg tttcagcagt cagttctttc
     4861 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca
     4921 aagatatcta cgcctgtacc agcaacagtc gcatagtttt taccgttgag gcgaccagtg
     4981 atcgtgtttt tagtagcaat cacttcttca aaagacaagt ccttttcagc agcaactagc
     5041 aaggttttag ttgggataca accaatgttg atacaagttc caccgtacat agccttgctg
     5101 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
     5161 gctttaccaa atccaataac aattaaatcg tatgttaaca tttatcttcc tcctattcga
     5221 atttcattct agcacaagaa aaaaactttt gcacaattct gctacggaaa agacagaaaa
     5281 agataaaaac gctttccaga caaaaaacta gtaatttttt gacaagcttg ataaactaaa
     5341 atcctatcta tttttatata agaaagaata aatgttcgaa aataggaggt tttatgaaat
     5401 cgaatcgtta tattattgcc tttgctgggg tcattttaca cttaatgctg ggttcgactt
     5461 atgcttggag tgtttatcgt aaccctatta ttgaaaaaac gggatgggat caggcttctg
     5521 ttgccttcgc ctttagtcta gcaatctttt gtttgggctt atcggctgca tttatggggc
     5581 gtttggtaga aaaatttggt ccgaaagtca tggggagtct atctgctttt ctatacgcag
     5641 gtggaaatat cttaacagga tttgcaatag accgtcagga actgtggttg ttgtatctag
     5701 cttatggcat tttaggtggg cttggtttgg gagcaggcta tattacccct gtgtcaacga
     5761 ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg tttagcgatt atggggtttg
     5821 gttttgcttc tttattgact agtcccatag cgcaacacct catcgcaggg gtagggattg
     5881 tagaaacttt ttatatttta ggagcaagtt actttattat catgctccta gcttcacaat
     5941 tcattaagcg tccaaatgag caagagcttg caattttatc ttcttcaggg aaagaaaaaa
     6001 cagcctcttt gacgcaagga atggctgcaa atcaggctct aaaaagcaat cggttttata
     6061 tgctttggat tattttcttt atcaacatag cttgtggttt aggcttaatt tcagcggcat
     6121 cgccaatggc acaggagatg gctggcttgt ctacaagtca tgcagcagta atggtgggtg
     6181 ttttggggat tttcaatgga tttggtcgct tgctctgggc gagtttgtct gactatatcg
     6241 gtcgccctct aacctttagt atattactgc ttgtcaatct tttctctctc actttggctc
     6301 tttacagatt ccgttttatt tgtagttgct atgtctattt tgatgacttg ctatggagct
     6361 ggtttttctt tgattccagc ttatctcagt gatatttttg gaaccaagga attggccgct
     6421 ctgcatggtt atattttaac agcttgggca atggctggtt tagcgggacc tattttatta
     6481 gcagagactt ataaaatggc tcattcgtac acacaaacct tgttcgtttt tctcatttta
     6541 tacagtatcg ccttggcttt gtcttattat ctaggtcgtt caatcaaaaa agaaagtcaa
     6601 aaaccgctta catgattttc tgtagaaatg gggctatctt ttgctctggt cttgtgttat
     6661 actagatagg ttgcaaagaa aacagtactt ttcttttgtg gaaaagaagc aacacgattt
     6721 tatatctagt tatatgaaaa tacgtcataa aaagaaaagt ataaactaag ccctataggg
     6781 tggcttcgca ccacctttag aaagaagaat aacgtgaaat ttaatgaatt aaacttgtct
     6841 gctgatttgc tagcagaaat tgaaaaagct ggttttgtag aagctagtcc tatccaagaa
     6901 caaactattc ctttggccct tgaaggcaag gatgttatcg gtcaagctca gactggtaca
     6961 ggaaaaactg cagcctttgg cttgcctacc cttgaaaaaa tccgtacaga agaagcgact
     7021 atccaagctt tggtcatcgc tccaactcgt gaactagctg tccaaagtca agaagaactc
     7081 ttccgctttg gtcgtagtaa gggagtcaaa gtccgttcag tatatggcgg atcaagcatt
     7141 gaaaaacaaa ttaaggctct taaatctggt gcccatatcg tggtgggaac tccaggtcgc
     7201 ctcttggact tgattaaacg caaggccttg aaattacaag acattgaaac ccttatcctt
     7261 gacgaagcgg atgaaatgct taacatgggc ttccttgaag acatcgaagc cattatttcc
     7321 cgtgtacctg agaaccgtca aactttgctt ttctcagcaa ctatgccaga tgccatcaaa
     7381 cgtatcggtg ttcagtttat gaaagcccct gaacatgtca agattgcggc taaggaattg
     7441 acaacagaat tggttgacca gtactatatc cgtgttaagg aacaagaaaa atttgacacc
     7501 atgactcgtc tcatggatgt ggcacaacca gaactcgcta ttgtatttgg tcgtaccaaa
     7561 cgccgtgtgg atgaattgac tcgtggtttg aaaattcgtg gcttccgtgc agaaggaatt
     7621 catggcgacc tagaccaaaa caaacgtctt cgtgtccttc gtgactttaa aaatggcaat
     7681 cttgatgttt tggttgcgac agacgttgca gcgcgtggtt tggatatttc aggtgtgacc
     7741 catgtctaca actacgatat tccacaagat cctgagagtt atgttcaccg tatcggtcgt
     7801 acaggtcgtg ctggtaagtc aggtcaatct attacttttg ttgctccaaa cgaaatgggt
     7861 taccttcaaa tcattgaaaa cttgactaag aaacgcatga aaggtctcaa acctgcaagt
     7921 gtagaagaat ccttccaatc aaaaaaacag gtagctctca agaaaatcga acgtgatttt
     7981 gcagatgaaa ccattcgtgc caactttgag aaatttggta aggatgctcg caaattggct
     8041 gctgagttta ctccagaaga attggcaatg tatatcttga gtctgacagt ccaagaccca
     8101 gatagccttc cagaagtgga gattgcacgt gaaaaaccac taccgtttaa accatcaggt
     8161 aatggtttcg gtggtaaagc taagggaggt cgtggaggcc gtcgtgggga cgaccgtcga
     8221 gagcgtgatc gccgtggcaa tggtcgccgt gatgagttca aaaaaggaag tcgtggcaac
     8281 gatcgttttg ataaggaaaa acgttaccgt aaggataata aaaaaccacg caatacttta
     8341 agcgaaaagc aaacaggctt tgttattcgt aacaaaggcg ataaataaga aaaagcggtt
     8401 caaaatgaat cgctttttta tataaggaga ccgaatgtga aaagacaaat aaaattacta
     8461 tattatcttt tataatgtta tgatacacaa aaataggcaa cttgtcaata gaaaaggaac
     8521 aatttaatta aaaatattct ggtttttaat taaattgtaa gtcaatgcaa gtcaataagg
     8581 aattttcaga taattattga aaaagagacg tttttatgtt ataatgatag cgattaaatt
     8641 cgaggtgaaa aatggcacat ttattagaaa aaactagaaa aattacatca attttgaaac
     8701 gctcagagga gcagttgcag gatgagctcc cttacaacgc tattacgcgt cagttagcgg
     8761 atattattca ttgcaatgcc tgcattatca atagtaaggg acgtctgctt ggctatttta
     8821 tgcgttataa aacaaataca gatcgcgtag agcaattctt ccaaactaag attttcccag
     8881 atgactacgt tcaaggggct aatatgattt acgaaacaga agcaaacttg cctgttgagc
     8941 atgatatgag tattttccct gttgagagta gagatgattt tccagatggc ttgacgacta
     9001 ttgcaccgat tcatgtatcg gggattcgcc ttggttcttt gattatttgg cgtaatgata
     9061 aaaaattcga agatgaggac ttggttcttg ttgagattgc cagtaccgtt gttgggattc
     9121 agcttcttaa cttccaacgt gaagaagatg agaaaaatat tcgtcgtcgt actgctgtca
     9181 ccatggcggt caataccctt tcttactccg aactccgtgc tgtttcagca attttagggg
     9241 aattaaatgg aaatgaaggg aagttgactg cgtcagtgat tgcagatcgt atcggaatca
     9301 ctcgctctgt gattgtcaat gctcttcgta aacttgagtc tgcggggatt attgaaagtc
     9361 gctcacttgg aatgaaggga acctatctta aggtcttgat ttcagatatt tttgaagaag
     9421 tgaagaaaag agattactaa tgacaaaggc tttaatttcg attgattata cagaagattt
     9481 tgttgctgat agtgggaagc tgacagcagg tgctccagct caggcgattt cggatgccat
     9541 cagcaaggta actcgattag cttttgaacg aggagattat atcttcttta ctattgatgc
     9601 tcatgaagaa aacgattttt tccatccgga aagtaagtta tttcctcctc ataatctgat
     9661 tgggacgagt ggacggaatt tatatggaga tttggggatc ttttatcaag agcatggttc
     9721 agacagtcgt gtcttttgga tggataaacg ccattactca gctttttcag ggactgacct
     9781 agatattcgt ttgagagagc gtagagtgtc taccgttatc ttaacaggtg tcttgacgga
     9841 tatctgtgtc ctacatacag ctatagatgc ctataatcta ggatatgaca tcgagattgt
     9901 taaaccagct gttgcttcca tctggcctga aaatcatcaa tttgccctag gtcatttcaa
     9961 aaatacactc ggagctaagt tagtagatga aaatctaaat gaactttctg agtaacctgg
    10021 ttggtgaaaa tgaaaaaatt tgaaaaaaag tgttgacaag catcccaaaa gttgatatac
    10081 tagtaaagta atcgatgcgg ggatggcgga attggcagac gcgcaggact aaggatcctg
    10141 tgaccgcttt aggtcgtgag ggttcaagtc cctctctccg cataggaaaa gagttagcta
    10201 aagctagctc ttttgtattt ctctaaaaaa ttagaatcca gtcaaatgta gtggagaatc
    10261 agcgaaaata tgggaaaact gttccttgtt tctgcttttg tcatagaaga ataataaaga
    10321 ttatcttctt caaattctcc gatattctct aaagttttgt gcaagttgca cagaactttt
    10381 ttattttttt ggtcatcttg ccatagaaat ataaagcgtt ttcatctata atataattat
    10441 caaaagacaa aaggagttca cctcatggta gaattgaatc ttaaaaatat ttacaaaaaa
    10501 tatccaaaca gcgaacacta ttcagttgaa gatttcaact tgaacatcaa agataaagaa
    10561 tttatcgttt tcgtaggacc ttcaggatgt ggtaaatcaa ctacactccg tatgattgct
    10621 ggtcttgaag acattacaga aggtactgca tctatcgatg gcgtggttgt caacgacgta
    10681 gctccaaaag accgtgatat cgccatggta ttccaaaact acgctcttta cccacacatg
    10741 actgtttatg acaacatggc tttcggtttg aaattgcgta aatacagcaa agaagacatt
    10801 aacaaacgtg ttcaagaagc agctgaaata cttggattga aagaattctt ggaacgtaaa
    10861 ccagctgacc tttcaggtgg tcaacgtcaa cgtgttgcca tggggcgtgc gattgtccgt
    10921 gatgcgaaag tattcttgat ggacgaacct ttgtcaaact tggatgccaa acttcgtgta
    10981 tcaatgcgtg ctgaaatcgc taaaattcac cgtcgtatcg gagctacaac tatctatgta
    11041 actcacgacc aaacagaagc gatgacactt gcagaccgta tcgttattat gtcagctact
    11101 aagaaccctg ctggtacagg tactatcgga cgtgtagaac aaatcggtac tcctcaagaa
    11161 gtttacaaaa atccagttaa caaattcgtt gcaggattca tcggaagccc agctatgaac
    11221 ttcatcaacg tgaaattggt tggtagcgaa attgtttctg acggtttccg tttgaaagtg
    11281 ccagaaggag cattgaaagt tcttcgtgaa aaaggctacg aaggaaaaga attgatcttt
    11341 ggtatccgtc cagaagacgt gaatgcagaa cctgctttcc ttgaaacatt cccagactgt
    11401 gttgtaaaag cgactatctc tgtatcagaa ctgcttggtt cagaatctca cctttactgc
    11461 caagttggta aagacgagtt tgttgcaaaa gttgatgctc gtgactactt gcaaacaggt
    11521 gcaacagttg agcttggatt tgacttgaac aaagcacact tcttcgatgt agaaactgaa
    11581 aaaacaatct actaaaataa ataaaattca aagcactaca agaaaagata tctctttatc
    11641 aattgtagtg gagagatatc agttaatcta gggagagaaa caaaatgctt ctctcctttt
    11701 tgctagggaa gtcatattat gcatctatat tgtgatgctc tttaatactc ttcgaaaatc
    11761 tcttcaaacc acgtcaacgt cgccttgccg tacgtatgat tactgatttc gtcagtttta
    11821 tctgcaacct caaagatgta ctttgagcag cttacggcta gtttcctagt ttgctctttg
    11881 atttccattg agtattattt gtgggtacca tctacaagtg aagctatatg cgtaaactac
    11941 gtgagcaatt gaattcgaac tagagaggta ataataaatt tatgctatag ttatggtgac
    12001 ttgtatgctt ttgattctag tttatcaaat aatagattag aattgtcaga taatatcatt
    12061 ttgtgttata atgaagaaaa aacagaggtg ttcaaatgtc agaagcaggt cataagtttt
    12121 tagcaaaatt ggggaaaaaa cgcttacgtc caggtggaaa gcgtgctaca gattggttaa
    12181 ttgcagaagg aggattgtca aaagaaaaga gaatactaga ggttgcgtgt aataggggaa
    12241 ctacagcaat tgagttggca cagcgttttg gttgcaagat aactgctgtt gatatggatg
    12301 ctcaagcttt agaagtggct aaaaaatctg ctggaacggc aggtgttgct catttaatca
    12361 gttttgaaag agcaaatgca atgaaacttc cttatcaaga tgctagtttt gatattgtta
    12421 taaatgaagc tatgctgact atgcaagccg atcaagctaa gaaaaaatgt gtaatggaat
    12481 atctaagggt attaaaacct ggaggtcttc ttttgacaca tgatgtgctt cttaaggaag
    12541 ctaaagagtc tatcagacag gaattatcac aagcaattca tgtaaatgta ggtcctttaa
    12601 ctcaagatgg ttgggaacag gtgatgatag aatcaggtta ttgtgatgtg aaagtattga
    12661 ctggtgaaat gacattaatg aaattatcgg gtatgattta tgacgaaggt tggctaggaa
    12721 ctttgaaaat ttgtgtaaat gcttgtaaaa aggagaatag aaagcagttt ttaactatgt
    12781 ataaaatgtt tgctaagaat aaacagaaat tgggctttat tgcgatggct agttataaat
    12841 cgtcaaaacg ttagataatt attgaagtta acttttcctt ttttctttct taaaaaatat
    12901 gctataatag agagtgaaaa actttgaaag aaagaaaaag atgaatttaa aagattacat
    12961 tgcaacaatt gaaaattatc caaaggaagg cattaccttc cgtgatatta gtcctttgat
    13021 ggctgatgga aatgcttata gctacgctgt tcgtgaaatc gttcagtatg ctactgacaa
    13081 gaaagtcgac atgatcgtgg gacctgaagc tcgtggattt atcgtgggtt gtccagttgc
    13141 ctttgagttg ggaattggtt ttgcgcctgt tcgtaagcca ggtaaattgc cacgcgaagt
    13201 tatttctgct gactatgaaa aagagtacgg tgtcgatacc ttgactatgc acgcggatgc
    13261 cattaagcca ggtcaacgtg ttcttattgt agatgacctt ttggcgacag gtggaactgt
    13321 taaggcaact atcgagatga ttgaaaaact tggtggtgtt atggcaggtt gtgccttcct
    13381 tgttgaattg gatgaattga acggccgtga aaaaatcggt gactacgact acaaagttct
    13441 tatgcattat taatgaaaac agtccctagg gctgttttct ctacactagg atataaaaat
    13501 agactataac tagttagaga aaaactataa ttgaaaacta tatcttcttg cagtataata
    13561 aaaggactaa gtgtttgaga tttgtcttca aacacatgca attattcctg agagagtaca
    13621 gttaggagag ggttatgccg attcgaattg ataaaaaatt gccagctgtt gagattttac
    13681 ggacagagaa tatctttgtc atggatgatc aacgtgctgc ccaccaagat atccgtcctt
    13741 tgaagatttt aattttaaat ctcatgccac agaaaatggt cacagagacc cagttgttgc
    13801 gtcacttggc taatacaccc ctacaactgg atattgattt tctctatatg gagagccacc
    13861 gttctaaaac aactcgttca gagcacatgg agaccttcta taaaactttt cctgaagtca
    13921 aggatgagta ttttgatggg atgatcatca cgggtgctcc agttgagcat ttaccatttg
    13981 aggaagtgga ctattgggag gaatttaggc agatgcttga gtggtctaag acacatgtct
    14041 attcgaccct tcatatctgt tggggggctc aggctgggct ttatctgcgc tatggtgtag
    14101 aaaaatacca gatggacagt aagctatcag gtatttatcc tcaggacacc ctaaaagagg
    14161 gtcaccttct atttagaggc tttgatgata gctatgtatc ccctcattca cggcacacgg
    14221 agatttctaa ggaagaggtc ttaaacaaga ccaatctcga gattttatca gaaggacctc
    14281 aggttggggt ttctattttg gccagtcgtg atttacgaga aatttatagt tttggtcatt
    14341 tggagtatga ccgtgatacc ttggcaaaag agtattttcg agatcgtgat gcaggtttcg
    14401 acccacatat tccagaaaat tactttaaag atgatgatgt taatcaggta ccttgtcttt
    14461 gttggtcttc atctgcagcc ctctttttca gtaattgggt aaaccatgcg gtctatcagg
    14521 agacgccttt tgattggaga aagatagaag atgatgcatc tgcatatggg tatttataag
    14581 aggaattatg acatatttag acgcttttaa atcaggtacc ttggttttac cgagtgccct
    14641 gctcttgcat tttaaggaac tctttccttc tagcgacgat tttctggttt ggcaattttt
    14701 ctatttgcaa aatacgacag gcttagaaga aatgtcgcca agccagattg ctgaaaggat
    14761 tggcaaggaa atttcggatg tcaaccagtc catttctaat ctgacggaaa ggggactgct
    14821 ccagtatcgt actatcgaat taaatggcga aattgaattg ctctttgatg ctagtttggc
    14881 cttggaacgt ttggatgacc tgtttggagc agttcgttca agttcagacc agctaacacc
    14941 tcaaaaccag ctcaaggatt tggtggaaac cttccagcag gagttgggac gattgttgac
    15001 gccttttgag attgaggatt tgaccaagac actaaaggaa gatggaacca gtgctgactt
    15061 gattaaggag gctcttcgtg aagctgtttt gaatggaaaa ccaaactgga agtacattca
    15121 ggcgattttg agaaactggc gccatgaagg aatcaagagt gtggctcaaa ttgaggccaa
    15181 gagagcagaa agagaagcaa gcaatcctca gttgacacag gtatctgcag atttcagaaa
    15241 tgccatggat ctctggaagg attaatccat gcaagtaggc ttgaaatccg agtaagattt
    15301 gcaagctgtg tataattgtg atagaataaa tagaaaataa attgaaaaaa gaggtatgtg
    15361 aaatgtcacg taaaccattt atcgctggta actggaaaat gaacaaaaat ccagaagaag
    15421 ctaaagcatt cgttgaagca gttgcatcaa aacttccttc atcagatctt gttgaagcag
    15481 gtatcgctgc tccagctctt gatttgacaa ctgttcttgc tgttgcaaaa ggctcaaacc
    15541 ttaaagttgc tgctcaaaac tgctactttg aaaatgcagg tgctttcact ggtgaaacta
    15601 gcccacaagt tttgaaagaa atcggtactg attacgttgt tatcggtcac tcagaacgcc
    15661 gtgactactt ccatgaaact gacgaagata tcaacaaaaa agcaaaagca atctttgcga
    15721 acggtatgct tccaatcatc tgttgtggtg aatcacttga aacttacgaa gctggtaaag
    15781 ctgctgaatt cgtaggtgct caagtatctg ctgcattggc tggattgact gctgaacaag
    15841 ttgctgcctc agttatcgct tatgagccaa tctgggctat cggtactggt aaatcagctt
    15901 cacaagacga tgcacaaaaa atgtgtaaag ttgttcgtga cgttgtagct gctgactttg
    15961 gtcaagaagt cgcagacaaa gttcgtgttc aatacggtgg ttctgttaaa cctgaaaatg
    16021 ttgcttcata catggcttgc ccagacgttg acggtgccct tgtaggtggt gcgtcacttg
    16081 aagctgaaag cttcttggct ttgcttgact ttgtaaaata atcagtaagt agcaaaagct
    16141 aggtggaaca gcattcagat gtctgttaca ttttttatag gagaggaaag attgaaaaca
    16201 aaaattggat tagcaagtat ctgtttacta ggcttggcaa ctagtcatgt cgctgcaaat
    16261 gaaactgaag tagcaaaaac ttcgcaggat acaacgacag cttcaagtag ttcagagcaa
    16321 aatcagtctt ctaataaaac gcaaacgagc acagaagtac agactaatgc tgctgcccac
    16381 tgggatgggg attattatgt aaaggatgat ggctctaaag ctcaaagtga atggattttt
    16441 gacaactact ataaggcctg gttttatatt aattcagatg gtcgttactc gcagaatgaa
    16501 tggcatggaa attactacct gaaatcaggt ggatatatgg cccaaaacga gtggatctat
    16561 gacagtaatt acaagagttg gttttatctc aagtcagatg gggcttatgc tcatcaagaa
    16621 tggcaattga ttggaaataa gtggtactac ttcaagaagt ggggttacat ggctaaaagc
    16681 caatggcaag gaagttattt cttgaatggt caaggagcta tgatgcaaaa tgaatggctc
    16741 tatgatccag cctattctgc ttatttttat ctaaaatccg atggaactta tgctaaccaa
    16801 gagtggcaaa aagtgggcgg aaaatggtac tatttcaaga agtggggcta tatggctcgg
    16861 aatgagtggc aaggcaacta ctatttgact ggaagtggtg ctatggcgac tgacgaagtg
    16921 attatggatg gtgctcgcta tatctttgcg gcctctggtg agctcaaaga aaagaaagat
    16981 ttgaatgtcg gctgggttca cagagatggt aaacgctatt tctttaataa tagagaagaa
    17041 caagtgggaa ccgaacatgc taagaaaatc attgatatta gtgagcacaa tggtcgtatc
    17101 aatgattgga aaaaggttat tgatgagaac gaggtggatg gtgtcattgt tcgtctaggt
    17161 tatagcggta aagaagacaa ggaattggcg cataacatta aggagttaaa ccgtctggga
    17221 attccttatg gtgtctatct ctatacctat gctgaaaatg agaccgatgc tgagagtgac
    17281 gctaaacaga ccattgaact tataaagaaa tacaatatga acctgtctta ccctatctat
    17341 tatgatgttg agaattggga atatgtaaat aagagcaaga gagctccaag tgatacaggc
    17401 acttgggtta aaatcatcaa caagtacatg gacacgatga agcaggcggg ttatcaaaat
    17461 gtgtatgtct atagctatcg tagtttatta cagacgcgtt taaaacaccc agatatttta
    17521 aaacatgtaa actgggtagc ggcctatacg aatgctttag aatgggaaaa ccctcattat
    17581 tcaggaaaaa aaggttggca atatacctct tctgaataca tgaaaggaat ccaagggcgc
    17641 gtagatgtca gcgtttggta ttaagcgatg atttgaaaga gggatgtgat agtagcaccc
    17701 tctttttctt tgttttatga tagttcatcc tcgagtaaat tcaagttctt gctcggaaat
    17761 gaagcttata tagtagattg aatatagaca aataccttgt gattggtaaa acattttaga
    17821 aattcattta cctttcctaa tcgacttggt ttcatcttat ttcaatctat tatagtattg
    17881 gggaatttct tcaaaccaca tcagcttggt cagttctacc tgcgacctca aaacttgtgc
    17941 tttggtcaag ctgggtttag tttcctagtt tgctgatgga tttccattga ctataagcat
    18001 ccaaccctct ttttgtcttc taaagaattc ttaaattatc agtctattgc aacttttctc
    18061 atataagttc tttgtcttgc tattggtttt ccttagtagt atactaaggt agtaatcatt
    18121 aagaagtggt tacaaaaaat aatgaatgag gtaaagaaaa tggtagaatt gaaaaaagaa
    18181 gcagtaaaag acgtaacatc attgacaaaa gcagcgccag tagcattggc aaaaacaaag
    18241 gaagtcttga accaagctgt tgctgatttg tatgtagctc acgttgcttt gcaccaagtg
    18301 cactggtata tgcatggtcg tggtttcctt gtatggcatc caaaaatgga tgagtacatg
    18361 gaagctcttg acggtcaatt ggatgaaatc agtgaacgct tgattacact cggtggaagc
    18421 ccattctcta cattgacaga gttccttcaa aatagtgaaa tcgaagaaga agctggtgaa
    18481 taccgtaatg ttgaagaaag cttggaacgt gttcttgtta tctaccgtta cttgtcagaa
    18541 cttttccaaa aaggtttgga tgtcactgat gaagaaggtg acgatgtgac aaacggtatc
    18601 tttgcaggcg ctaaaactga aacagataaa acaatttgga tgcttgcagc cgaacttgga
    18661 caagcacctg gtttgtaaga aataaggaaa catagataaa tctgtaggaa atttcttaca
    18721 gattttttct attttgtaag ctattccaaa ccagtctttt tttgagtttt ttgataaaat
    18781 agtactatca gtgaaaagga tggaagcatg actaagaaaa tcgtagctat ttgggcccag
    18841 gatgaagagg gtgtgattgg taaggaaaat cgtctgcctt ggcatttgcc agcagaattg
    18901 cagcacttta aagaaacaac tctgaattat gctatcttga tggggcgtgt gacctttgat
    18961 gggatggggc gtcgcttgct tccaaaacgg gaaaccttga ttttgacgcg taatccggaa
    19021 gaaaagatag atggggttgc tacttttcag gacgtccagt ctgttcttga ctggtatcag
    19081 gctcaagaaa agaatctcta cattatcggt gggaagcaaa tttttcaggc ttttgaacct
    19141 taccttgatg aagtgattgt cactcacatt catgctcggg tggaaggaga tacctatttc
    19201 cctgaagaac ttgacttgtc tctttttgag acagtttcaa gcaaatttta cgccaaagat
    19261 gagaagaatc cttatgattt taccatccaa taccgcaaga gaaaggaagt ctaatggaac
    19321 ggagtttatt tggtctattc acggcttttc tgtgtttcat ctgctttctg gcaggagcac
    19381 aagcttttcg taaaaagcgt tacgggcttt ctatcctcct ttggttaaat gcctttacca
    19441 atctggtcaa tagtattcat gctttttaca tgaccttatt ttagatagaa tgacagtata
    19501 gaatggaagg aaatcatgtc tacaaataga aaaaatgata tgatggttta ttgctcattt
    19561 tgtggcaaaa accaagaaga agtacaaaaa ataattgctg gcaacaatgc ttttatttgt
    19621 aatgaatgcg tggagttagc tcaggaaatc attcgagaag aattggttga ggaagtcttg
    19681 gcagacttgt ctgaggtgcc aaaaccaatt gaactcctcc atatcttgaa ccactatgta
    19741 attggtcaag atcgtgccaa gcgtgccttg gcagtggcgg tttataacca ctacaaacgc
    19801 atcaatttcc acgatacacg cgaagagtca gaagatgtgg atttgcagaa gtcaaacatt
    19861 ttgatgattg gcccaactgg ttcagggaaa actttccttg cccagacctt ggctaagagc
    19921 ttgaatgtac cttttgctat tgcggatgcg acagctctga cggaggctgg ttatgtgggt
    19981 gaggatgtgg aaaatatact cctcaaactc ttgcaggttg ctgactttaa catcgaacgt
    20041 gcagagcgtg gcattatcta tgtggatgaa attgacaaga ttgccaagaa gagtgagaat
    20101 gtgtctatca cacgtgatgt ttctggtgaa ggggtgcaac aagcccttct caagattatt
    20161 gagggaactg ttgctagcgt accgcctcaa ggtggacgca aacatccaca acaagagatg
    20221 attcaagtgg atacaaaaaa tatcctcttc atcgtgggtg gtgcttttga tggtattgaa
    20281 gaaattgtca aacaacgtct gggtgaaaaa gtcatcggat ttggtcaaaa caataaggcg
    20341 attgacgaaa acagctcata catgcaagaa atcatcgctg aagacattca aaaatttggt
    20401 attatccctg agttgattgg acgcttgcct gtttttgcgg ctcttgagca attgaccgtt
    20461 gatgacttgg ttcgcatctt gaaagagcca agaaatgcct tggtgaaaca ataccaaacc
    20521 ttgctttctt atgatgatgt tgagttggaa tttgacgacg aagcccttca agagattgct
    20581 aataaagcaa tcgaacggaa gacaggggcg cgtggacttc gctccatcat cgaagaaacc
    20641 atgctagatg ttatgtttga ggtgccgagt caggaaaatg tgaaattggt tcgtatcact
    20701 aaagaaactg tcgatggaac ggataaaccg atcctagaaa cagcctagag gtgactatgg
    20761 aacttaatac acacaatgct gaaatcttgc tcagtgcagc taataagtcc cactatccgc
    20821 aggatgaact gccagagatt gccctagcag ggcgttcaaa tgttggtaaa tccagcttta
    20881 tcaacactat gttgaaccgt aagaatctcg cccgtacatc aggaaaacct ggtaaaaccc
    20941 agctcctgaa cttttttaac attgatgaca agatgcgctt tgtggatgtg cctggttatg
    21001 gctatgctcg tgtttctaaa aaggaacgtg aaaagtgggg gtgcatgatt gaggagtact
    21061 taacgactcg ggaaaatctc cgtgcggttg tcagtctagt tgaccttcgt catgacccgt
    21121 cagcagatga tgtgcagatg tacgaatttc tcaagtatta tgagattcca gtcatcattg
    21181 tggcgaccaa ggcggacaag attcctcgtg gtaaatggaa caagcatgaa tcagcaatca
    21241 aaaagaaatt aaactttgac ccaagtgacg atttcatcct cttttcatct gtcagcaagg
    21301 cagggatgga tgaggcttgg gatgcaatct tagaaaaatt gtgaggaaaa gaaaatggca
    21361 aaaacaattc atacagataa ggccccaaag gctatcggac cctatgttca aggaaaaatc
    21421 gttggcaacc ttttgtttgc tagcggtcaa gttcccctat cccctgaaac tggggaaatt
    21481 gtaggagaga atatccaaga acagacagag caagtcttga aaaacatcgg tgctattttg
    21541 gcagaagcag gaacagactt tgaccatgtt gtcaaaacaa cttgcttctt gagcgatatg
    21601 aacgactttg ttccttttaa tgaggtttac caaacggcct tcaaagagga attcccagct
    21661 cgttcagctg tggaggtagc tcgtcttcct cgtgatgtaa aagtcgaaat tgaagtcatc
    21721 gcagagattg gataagctag ttgaagtttg gtgttgccaa acttcttttg atataaggag
    21781 aaaaagatga caaagaaaca acttcacttg gtgattgtga cagggatgag tggcgcaggg
    21841 aaaactgtag ccattcagtc cttcgaggat ctaggttatt tcaccattga taatatgccg
    21901 ccagctctct tgcctaagtt tttgcagctg gttgaaatta aggaagataa tcctaagttg
    21961 gctttggtag tggatatgcg tagccgttct ttcttttcag agattcaagc tgttttggat
    22021 gagttggaaa atcaagatgg tttggatttc aaaatcctct ttttggatgc ggctgataag
    22081 gaattggtcg ctcgttacaa ggaaaccaga cggagtcacc cactagcagc agacggtcgt
    22141 attttagatg gaatcaagtt ggaacgtgaa ctcttggcac ctttgaaaaa tatgagccaa
    22201 aatgtggtgg atacgactga actcactcca cgtgaactgc gcaaaaccct tgcagagcag
    22261 ttttcagacc aagaacaagc ccagtctttc cgtatcgaag tcatgtcttt cggatttaag
    22321 tatggaatcc cgattgatgc ggacttggtc tttgatgtcc gtttcttgcc aaatccctat
    22381 tatctaccag aactgagaaa ccaaacgggt gtggatgaac ctgtttatga ttatgtcatg
    22441 aaccatcctg agtcagaaga cttttatcaa catttattgg ccttgattga gccgattctg
    22501 ccaagttacc aaaaggaagg taagtccgtt ttgaccattg ctatgggatg tacgggtgga
    22561 caacaccgta gtgtggcatt tgctaaacgc ttggcgcagg acttatccaa gaattggtct
    22621 gttaatgaag ggcatcgcga caaagaccgc agaaaggaaa cggtaaaccg ttcatgagaa
    22681 aaccaaagat aacggtgatt ggtggaggga ctggaattcc cgtcattcta aaaagtctgc
    22741 gggaaaaaga tgtggaaatc gcagctatcg tgacggtggc agatgatggt ggttcttcag
    22801 gtgaactccg aaaaaatatg caacagttga caccgccagg tgatcttcgt aatgtccttg
    22861 tggccatgtc ggatatgcct aagttttatg agaaggtctt tcagtatcgg ttctctgagg
    22921 atgccggagc ctttgctggc catccattgg gaaatctcat cattgctggc ttgtcagaaa
    22981 tgcagggttc aacctataat gccatgcagt tattgagcaa atttttccat acaacaggga
    23041 aaatttatcc ttccagtgac catcctttga cccttcatgc agtctttcag gatgggacag
    23101 aagtggctgg agagagtcat attgtagacc atcgaggcat aattgacaat gtctatgtga
    23161 ccaatgccct aaacgatgat acgcctctgg ccagccgtcg agtagtgcag accatccttg
    23221 aaagtgacat gattgtccta gggccaggtt ccctctttac ctctattttg cccaatatcg
    23281 tgattaagga aattgggcgg gctcttttgg aaactaaggc agaaattgcc tatgtctgca
    23341 atatcatgac ccaacgtggg gagacggaac actttacaga tagcgaccac gtggaagtct
    23401 tgcatcgtca ccttggtcgc ccttttatcg acactgtctt ggtgaatatt gaaaaagtgc
    23461 ctcaggaata catgaattcc aaccgttttg atgaatactt ggtgcaagtg gaacacgatt
    23521 ttgtaggtct ttgtaagcaa gtttcgcgcg tgatttcatc taacttcctt cgtctggaaa
    23581 atggcggtgc cttccacgat ggagatttga ttgtggacga gttgatgcgc attatacagg
    23641 tgaaaaaatg agtttcacag tagcagtaaa agaagaaatc ctgggccaac accatctgag
    23701 ccggcatgaa ttatctgcca ttatcaaaat gtctggtagc atcggtctct cgacttcggg
    23761 cttgactttg tcagttgtga cagaaaatgc caaactggcc cgtcacctct atgagtcctt
    23821 tctccatttc tatgaaatca aatctgaaat tcgtcaccac caacggagca atcttcgcaa
    23881 gaatcgcgtc tataccgttt ttacagatga aaaggtgcag gatttgttaa gtgatttgca
    23941 cttggcagac tctttctttg gcctggaaac aggtattgat gaggcgattt tatcggatga
    24001 ggaagcaggt cgtgcctatc tctgtggcgc tttcttggca aatgggagca ttcgtgaccc
    24061 tgagtcaggc aagtaccagt tggaaatcag ttctgtttat ctggaccacg cgcaaggaat
    24121 tgcctccctt ctccaacagt ttttactgga tgccaaggtg cttgagcgca agaagggggc
    24181 tgtgacctat ctccagcgag cagaagacat tatggacttc ttgatagtaa tcggagccat
    24241 gcaggcacgt gatgattttg agcgggttaa gattttgcga gaaacccgta acgacctcaa
    24301 tcgggctaat aatgccgaga cagctaatat cgctcggaca gtttctgcca gcatgaagac
    24361 cattaacaat atcagtaaaa taaaagatat catgggctta gaaaatttgc cagtagattt
    24421 gcaggaagtg gcgcaactga ggattcagca cccagactac tctatccagc agttagcaga
    24481 tagtctcagc acccctctga ccaaaagtgg tgtcaatcac agactcagaa aaatcaataa
    24541 gatagcggat gaattataaa accacgaatc ctatgtgact cgtggttctt ttttataaac
    24601 tggtagagtg ttttggttgt actttttgtt cagggtcaat gtagttgata gcgttgttga
    24661 cagcagttgg agcttctccg aggcctgtcg caatcagatc aatttttccg tcatagtagc
    24721 agcagtcacc gatagcatag atacctgctt ggctggattc ctgtttgctg ttgacgataa
    24781 tcttgtgacg gttgaggtcg agcccccagt ttttaaggtt accgacagaa gatttgaaac
    24841 catagttgac aaagaggtgg tctaggtcaa tggtttcagt ttcatcagat ttgacttttg
    24901 tgatttcaag tttatcaagt gtttttccat ttccaaggag ttggctaggg gcgaatggtg
    24961 tcttgatggt tacagatgat tcttgcaagg cttgaacact gtgttccaag gcacggaaat
    25021 tatctctgcg gtgaacaagg gtagttggtg cgattttttc aaaagccaaa gtccaatcca
    25081 cagccgagtc tcccccacca agaatcgtca cttttttacc agcgtattgc tgaatgttag
    25141 aaacgtggta gtggatattt tcatagccct caaccccttc aagttccagc ggacgtggtt
    25201 tgaaggcacc gccacccata gcgatgataa ctgttttagt caggtgactt cctttagaag
    25261 ttgtgatggc aaattcttct tcttgtttgt caatctcaag aaccgtttca ttgagatgaa
    25321 taggggtatc aaatccattt agctgttcaa tcaagcggtt agtcaactct tctccagtca
    25381 ggtttgggaa gcctggtacg tctaggattt ccttttcagg gtagagaata gcaggttgtc
    25441 cacctagctg gggaagagag tcgatgattt gaaccttggc ttggcgtagg tgggcataaa
    25501 aggctgcaaa aagcccgaca ggaccaccac ccacaatggt aatatcatag agttgagaca
    25561 tagtttctcc tttgtttttt ctagtcagtt tattttatca tatttttgtt caatgtaaaa
    25621 tggactagga tagggaaaaa aatgccagaa aatggtataa taggacgaaa gtgttttgat
    25681 agggaggata tcagggatga attttcaaca attatccaat ttgcaatatt ggaccagttt
    25741 gtttgcaagt ccatggacga tagctatcaa tctgattgat attttgattg ttgcttatat
    25801 tttataccat tttacaaaag ctattgcagg aaccaagatt atgattttgg tacgtggagt
    25861 tttggtattt attttagctc aaatccttgc aaatatgatt ggtttgacta cgatttcttg
    25921 gttaatcaat caaattatta cttatggggt tattgcggcg gttgttatct tctctccaga
    25981 gattcggact ggtttggaac gtttgggaag agcgacagat ttcttttcca atgcccctat
    26041 tagtgctgag gaacagatga ttcgtgcctt tgttaagtct gttgaataca tgagtcctcg
    26101 taaaatcggg gccttggttg ctattcagcg tgtacgtacc ttgcaggagt atatttcgac
    26161 aggaattccc ttggatgcta agatttctgc agaacttctc attaacattt ttattcccaa
    26221 cactccccta catgacggtg cggtgattat caaagaagaa cgtatcgctg tgacgtctgc
    26281 ctatctgccc ttgacaaaaa acacagggat ttccaaggaa tttgggacca gacaccgggc
    26341 ggctatcggt ttatcagaag tctcagatgc cttgactttt gtcgtatcag aggaaacggg
    26401 aggaatttcg ataacctata atggaaggtt taagcacaac ctaacacttg atgaatttga
    26461 aacagaatta cgtgaaatct tacttccaaa agaggaagta ggtcttagtt ttaaagaacg
    26521 attgctagga ggatggaaac atgaaaaaaa atagtttata tatcatatcc tcactctttt
    26581 ttgcttgtgt cttatttgtc tatgctacgg cgacgaattt tcaaaacagt accagtgcta
    26641 ggcaggtaaa aacggaaacc tatactaata cagtaacaaa tgtccctatt gacatacgct
    26701 ataatagtga taagtatttt attagcggtt ttgcttcaga agtatcagtg gtcttgactg
    26761 gtgcaaatcg cctatcgcta gctagtgaaa tgcaagaaag tacacgtaaa ttcaaggtta
    26821 ctgctgacct aacagatgcc ggtgttggaa caattgaagt tcctttgagc attgaagatt
    26881 tacccaatgg gctgaccgct gtggcgactc cgcaaaaaat tacagtcaag attggtaaga
    26941 aggctcagaa ggataaggta aagattgtac cagagattga ccctagtcaa attgatagtc
    27001 gggtacaaat tgaaaatgtc atggtgtcag ataaagaagt gtcgattacg agtgaccaag
    27061 agacattgga tagaattgat aagattatcg ctgtcttgcc aactagcgaa cgcataacag
    27121 gtaattacag tggttcagta cctttgcagg caatcgaccg caatggtgtt gtcttaccgg
    27181 cagttatcac tccgtttgat acaataatga aggtgactac aaaaccagta gcaccaagtt
    27241 caagcacatc aaattcaagt acaagcagtt catcggagac atcttcgtca acgaaagcaa
    27301 ctagttcaaa aacgaattaa aaatagaaaa ggattttata acaatgggta aatattttgg
    27361 gactgatgga gtccgtggag aagctaacct agaactaaca ccagaattag cctttaaact
    27421 aggacgtttt ggaggctatg ttcttagtca acatgaaacg gaagcgccga aagtctttgt
    27481 aggacgtgac acacgtattt caggggaaat gctagaatcg gccttggtgg caggtctcct
    27541 ttcagtaggg attcacgtat acaaacttgg tgtccttgca acaccagcag tagcttactt
    27601 ggttgaaact gaaggagcaa gtgctggtgt catgatttct gctagccaca atccagctct
    27661 tgataacgga atcaagttct ttggcggtga tggcttcaaa ctagatgatg aaaaagaagc
    27721 agaaattgaa gccttgctag atgctgagga agacactctt cctcgtccaa gtgcagaagg
    27781 cttaggaatt ttggtagatt atccagaagg cttgcgtaag tatgaaggat accttgtttc
    27841 aactggaact cctcttgatg gaatgaaggt tgccttggat acagctaatg gagcagcttc
    27901 taccagtgcc cgtcaaatct ttgcagacct tggtgcccaa ttgacggtta tcggggaaac
    27961 accagacggt cttaacatca accttaatgt tggttcaaca catccagaag cccttcaaga
    28021 agtggtcaaa gaaagtgggt cagctattgg tttggccttt gatggagaca gtgaccgctt
    28081 gattgctgtt gatgagaatg gtgacatcgt cgatggtgac aagattatgt acatcatcgg
    28141 aaaatacctt tctgaaaaag gacaattggc tcaaaataca attgtgacaa ctgttatgtc
    28201 taaccttggt ttccacaagg ccttgaatcg cgaaggtatt aacaaggcag ttactgcagt
    28261 tggtgaccgc tacgttgttg aagaaatgag aaaatcaggt tacaaccttg gtggtgaaca
    28321 gtctggtcac gttatcttga tggattacaa taccacaggt gatggtcaat tatcagcaat
    28381 tcaattgact aaaatcatga aggaaactgg taagagctta tcagagttgg cggcagaagt
    28441 aacgatttat ccacaaaaat tagttaatat ccgagtggaa aacgtcatga aggaaaaggc
    28501 catggaagtg ccagctatca aggccatcat cgagaagatg gaagaagaaa tggcggggaa
    28561 cggccgtatc cttgttcgtc caagtggaac agagcccctc ttgcgtgtta tggcagaagc
    28621 gcctacaaca gaagaagtgg actactatgt tgatactatc acagatgtag ttcgtgctga
    28681 aattgggatt gactaaatcc tagaaaacta gatgaaaata aaaaattatg gtacaatagc
    28741 ttataatatt gtagccgatg gagaaagaca tgaatcttaa acgtgaacaa gaatttgtta
    28801 gtcagtatca ttttgatgca cgtaattttg aatgggaaaa tgaaaatgga gctcctgaaa
    28861 ccaaggtgga tgtgaatttc caattgctcc aacatgacca agaaaatcaa gtgacttcct
    28921 tgattgtcat tttgagcttt atgattgtat ttgataagtt tgtcattagt ggaacaatct
    28981 cacaagtaaa ccatatcgat ggccgtattg tcaatgaacc aagtgaattg aaccaagaag
    29041 aggtggaaac cttggctcgt ccatgtttga acatgctcaa tcgtttgact tacgaagtga
    29101 ctgaaattgc attagactta ccaggaatta atttggagtt ctaatatgaa attagctgtt
    29161 ttcacagatt cctctgctta tctcagtgaa gagaccttgc aaagagaaga cttgtttgtc
    29221 ttggatattc ctgtcaatat tgatggtgag gaatatgtcg aaggcatcaa tctgtctgct
    29281 gaggaatttt accaaaaaat ggctcaggct tctgaattgc ctaagaccag tcaaccaagt
    29341 attgccaagt tagatgagat cttaacttcg ctcaaagaac aaggctatac acatgccttg
    29401 gggcttttcc tatcttctgg aatttcaggt ttttaccaaa atatccagta tatggtcgat
    29461 gactatgagg gcttaaccat tgctttccca gacactttga ttacaagtgc tcccctaggc
    29521 atcatggttg aaagcgtctt taattggcgt gatcagggcg atgattttgc cagcattcag
    29581 gataagctag ccattcaaat cagccgtacg tcagccttta tcatggtaga tgacttggat
    29641 catttggtaa aaggtggacg cctttcaaat ggggctgcca ttttgggcaa tttgcttagc
    29701 attaagccaa tcctttattt taacgatcaa ggtgtgattg aagtttacga aaaagttcgt
    29761 actgaaaaga aggccaccaa gcgcttaatt gaaattatca aggaaacaac ggcttcaggt
    29821 caataccggg tcattgtcat tcatgggaat gctcctgaaa aggctgaaga attgcgtcag
    29881 cacttgcttg attttggctt gggttcggat gtttcacttg ctacatttgg tagtgtcatt
    29941 ggaacgcacc taggagcagg aagtattgct ctgggttata ttccagtgat ttagttggcg
    30001 cttctaggct agttaagatg ccttgtatct tagaaattga acacggacta cctgcctctt
    30061 gaaaaaagat gcctgtcttg cctttcgtgg aaagtcagtg ccattcccta tttttcagag
    30121 gcagctaagg tcctttgtat cttgataatt gaacacgcct ggaaccctgt gtgaaaaaga
    30181 tagttcttcc aagaagtaga cacttcttga ttggaattcc tattttcact ttgtgttctt
    30241 acggactttg tatcttagac aggagtagag atgagtattc gagtaattat tgccggtttt
    30301 aagggaaaga tgggccaggc tgcttgtcag atggtattga ctgatccaga cttggacttg
    30361 gtggcagttt tggatccttt tgagtctgag tcagaatggc agggtattcc tgttttcaag
    30421 gataaggctg atttagctgg ttttgaagcg gatgtctggg tagattttac tactccagct
    30481 gttgcctacg aaaatacacg ttttgctctt gaaaatggct ttgctccagt agttggaacg
    30541 actggtttca cgagtgaaga aattgcagag ctaaaagaat tttctcgtgc ccaagacttg
    30601 ggtggcctga ttgcccctaa ctttgccttg ggtgctgtct tactcatgca atttgcgacg
    30661 caggctgcca aatatttccc aaatgtggag attattgagc tccatcatga caagaaaaag
    30721 gatgctccga gtggaacagc cattaaaaca gctgagttga tggcagaggt tcgagagtcc
    30781 attcagcaag gcgcagcaga tgaggaagag ctgattgctg gtgctcgtgg tgctgacttt
    30841 gatgatatgc gcatccactc agttcgtttg ccaggcttgg tagctcatca ggaagtcatc
    30901 tttggcaatc agggagaagg gttgaccctc cgtcatgact cctatgatcg catctccttc
    30961 atgacaggag tcaatttggg aattaaagaa gttgtcaagc gtcatgagct tgtctatgga
    31021 ttagaacact tattatgaga ttaacgcaaa tgccttctga atttcagaag gctttaccag
    31081 tattagaaaa aattaaagaa gcaggctttg aggcttattt tgttgggggc tctgttcgag
    31141 atgcccttct ccatagccct atccacgatg tggatattgc gacgtcttct tatccagaag
    31201 agaccaagca gatttttccg cgaacagccg atatcggaat cgagcatgga accgtcttgg
    31261 tcttagatgg ggatgaggag tatgaggtaa caacctttcg gacagaggat gtctatgtgg
    31321 actatcgcag acccagtgcg gtttcctttg tacgatcgct agaagaagac ctcaaacgcc
    31381 gtgatttcac agtcaacgcc tttgccttgg atgagacagg agaaatcgtt gacttgttcc
    31441 atggtttaga agatttggaa aagcaagtct tgcgagcagt tggagtggct agtgagcgtt
    31501 tcaacgaaga tgctttacgg attatgcgtg gtttccgttt tcaggctagt cttggttttg
    31561 cacttgagcc agaaacattt aaagctatga agaccttgac gccgcttttg gagaaaattt
    31621 ctgtagagcg taccttcgtc gagtttgata aactcttgct ggctccattt tggagaaggg
    31681 gcttggcttc catgattgag agtcaagctt atgactatct ccctgatatg gcatctagcc
    31741 aggacaagct caacagactg tttgatttgg agactgattt tacttttgaa tcctctgaac
    31801 aagcctgggc ggctttacta tgggctttgg agattgaaaa tgcgcagtca tttttgaaat
    31861 cttggaagac ctcacgccag tttgccaagc aagttcagga tttgctgatt attttggctc
    31921 tgcgtgaaaa tggagaattg agtaagcgag attgttatcg ctttgacata gatttgcttt
    31981 tacaggctga aaatcttcgt caagctcaag gaaaagaagt caacccacaa gccatcacag
    32041 aaaaatacca aagcttgact atccatgaca agaaagagat tcagattaat ggcgatattt
    32101 tgatcaagga atatggctat cagccaggcc cagacttggg agagatttta acagagattg
    32161 agtttgccat tgtcgatgga gaattggaga ataatcgtga agccatccat gcttacctga
    32221 gggagaaaaa atgagtgatt ttatcgttga aaaactaagt aaatccgttg gtgacaagac
    32281 cgtttttagg gatatttcct ttattatcca tgacttagac agaattggtt taatcggtgt
    32341 caatgggact ggcaagacca cccttttgga cgtcctttct ggtgtttctg gatttgatgg
    32401 ggatgtcagt cctttttcag ctaaaaatga ttaccagatt ggttacttga ctcaggatcc
    32461 tgattttgat gatagaaaga cagttttgga tacggttcta tctagtgaac tcaaggaaat
    32521 ccagctcatt cgtgagtatg aattgattat gctcgactat agtgaggaca agcaggcgcg
    32581 tttggaacgt gtcatggcag agatggactc tctccaagct tgggaaatcg aaagtcaggt
    32641 caagaccgtt cttagcaaat tgggcattca agacttatct actcctgttg gggaattgtc
    32701 aggtggtctg agaagacggg tacagttggc acaagtctta cttggcaacc acgacctctt
    32761 gcttttggat gagccgacca accatctgga tattgcgatt attgagtggc tgaccctctt
    32821 tttgaaaaat tctaagaaga ccgtcctttt tatcactcac gatcgttatt tcttagacgc
    32881 tttgtcaaca cggattttcg agttggatcg tgcaggcttg accgagtacc agggcaatta
    32941 ccaggactat gttcgcctaa aggcggaaca ggatgagcgc gacgcggctc ttcttcacaa
    33001 aaaagaacaa ctctacaaac aagaactggc ctggatgcgc agacaaccgc aggcgcgtgc
    33061 gaccaagcag caggcccgta tcaatcgatt ccatgatctg aaaaaggaag tttcaggcag
    33121 tagtgctgag acagacttga ccatgaactt tgaaaccagt cggattggga agaaagtcat
    33181 cgagtttcag gctgtttcct ttgcctatga aaacaagcct attttgcaag attttaatct
    33241 cttggtgcag gccaaagacc gtatcggaat cgttggggac aatggtgttg gaaaatcaac
    33301 cctgctcaac ttgattgcag gaagtcttga gccaactaaa ggacaagttg tgattgggga
    33361 aactgttcgc atcgcctatt tctctcagca aattgagggt ttggatgaaa gcaagagagt
    33421 tatcaattac ctgcaggaag tggcagaaga ggtcaagacc agtggcggtt ctacgacttc
    33481 gattgcagag ttgctggagc agttcctttt cccacgttcg acgcatggaa ccttgattga
    33541 gaaattgtca gggggtgaga aaaaacgtct ttatctcctc aaactgcttt tggaaaaacc
    33601 aaatgttctt cttttagacg agccaaccaa tgacctagat attgcaactt tgacagtctt
    33661 agagaatttc ttgcaaggtt ttgcaggtcc cgttttaaca gtcagtcacg accgctattt
    33721 cttggataag gtagcgacca agattctcgc ttttgaggat agcaagattc gtcctttctt
    33781 tggtcattac accgactatc ttgatgaaaa agcttttgaa acagatatgg ccaatcaagt
    33841 gcaaaaggtc gaaaaggaaa aagtggtcaa ggttcgagaa gacaagaaac gtatgaccta
    33901 ccaagaaaag caggagtggg caagtattga aggtgatatt gaaaccttgg aaaaacgtat
    33961 cgctgctatt gaagaggaaa tgcaggctaa cggctctgac tttggtaagc tggctactct
    34021 ccaaaaagaa ttggatgaga aaaatgaagc actccttgaa aaatacgaac gctatgagta
    34081 tctcagtgaa tttgatagtt aagactattc gaaaatcaaa gtgcaaacta tgtcggcttc
    34141 acattgccga ctcaagtaca gcctgtgacc ggcttcctaa tttgcacttt gatttttatt
    34201 gagtataaaa gaatagaaaa atcccgtctc atgaaaggat ttctctattc ttttttggtt
    34261 tcttgatgaa agatattttg ccaggtttcg tggcgacgcc agatactggt atggatgata
    34321 ccatctaact catagcagat gagtttggtt ttttgactga tggaagtaat ttgaatgtcc
    34381 ttgatagcag aatttaactc ttttttggtc ttataagcct ctttatccat ctgctctcca
    34441 tcttggcgaa tatagacaaa atcatcagtc aagagttctt ctagttggtt tccttggtca
    34501 atcaattgtt cacgcataag caatttttta tagacattgt ctaaaatttc atcctcagag
    34561 ataggtgctg gttcgatatg gacatcggta tcaaagacgc caaaacgctc ctccagcata
    34621 gactcgacct gatccgcgat ttcatggctt tcaaaaacag acaagtcagg attcatctct
    34681 agtgtaatat ccaggtagat gttgctaccg taggtgcgac ctctttgcga tttgaccttg
    34741 ctgattttgg gaatttccat gatagccttt tggtagtcct cgagcaggcg gtcgtcaaag
    34801 ccatctgaaa gactaaagga agactcgatg aagatatcat aggcagtctt caagataaag
    34861 aaagtgatga tgatagcaac cagtttatcc acaatcggat aattgaaact actagctagg
    34921 atggcaatgg cggttccaag tgaggtaaca gcgtcagaaa gattgtcctt agcagctgcc
    34981 ttcagcgcct tggagttgga tttcttactg aggcgagtat tgtagagata gaccacaaac
    35041 ataatcgctg cagaaatgat tcctagagtt gcaccaagag gatcaatgac cgtttcttcc
    35101 cgactgagaa tcttttgaat ggtatctctt agaacatcga aaccgacata gaacatgatg
    35161 atagaagtga tcaagcttgc caaatcttca atcttccaat gaccaaaacg gtggtcacgg
    35221 tctgcaggct ggcgcgccat ccgaatcccg attaagaggg ccacatttcc aatgatgtcc
    35281 gatacgttat taaaaccatc ggccaccaaa ctggatgaat gaaggagatg accagctgct
    35341 aatttggctg cagacaagat caaataggtc gaaatactga taatggctcc acgctcagct
    35401 aacttgagat ttgagataga ttgcttcatt caacttcctt tctagtcaca tagcattcta
    35461 ccattatact ggttttcaag gaaaaattca aataaggcag tttttactct ttgaaaactc
    35521 cccaaaattt ggtataatgg tactactcaa gggagtagct ggcagaaacc tgtgatagtg
    35581 tcgtcattcc gaattttatg ctgaaaagta tgctttccgg ccctatctta aacagcgaga
    35641 cttgttatga ttaacaggtc tctttttgtt tgtcgtgaaa agatatgaca gaaagagagt
    35701 ctgttttgca cacaatgtca aggaggagac acatgtcaaa agaacaaaaa cgccaagcgt
    35761 tttatactca gagccctgaa gaggtcttgc aggctgtgga tgcgaccgag caaggtttgt
    35821 catcaagtga ggcggaaaag cgccttgccg aatttgggca caatgaactc gaagaaggcg
    35881 agaaacgatc aatcttggtc aaattcatcg agcaatttaa ggatttgatg attatcatcc
    35941 tagttgcggc agcaatcttg tcagtcgtga cttctggtgg ggaagatatc gcagatgcca
    36001 ttatcatcct agctgtggtt atcatcaacg ctgcctttgg tgtttaccaa gaaggaaaag
    36061 ctgaagaagc tattgaagcc ctcaaatcca tgtctagtcc agttgcccgc gttcttcgtg
    36121 atggacacat ggcagagatt gactctaaag aattggtacc aggcgatatc gttgcccttg
    36181 aagcaggtga cgtggtacca gcggacctac gtttgataga agccaactct cttaaaattg
    36241 aagaagcagc cttgacaggt gaatctgtac cagtcgaaaa agacttgtca gtcgagcttg
    36301 cgacagatgc tggtattggt gaccgtgtca acatggcctt ccaaaactca aacgtaacct
    36361 atggtcgtgg gatgggtgtt gttgtcaata caggtatgta cactgaagtt ggtcatattg
    36421 ctggtatgct tcaagatgcg gatgagactg atacaccact caaacaaaat ttgaacaacc
    36481 tttctaaggt cttgacctat gctatcttgg tcattgccct tgttactttt gtagtgggtg
    36541 tcttcattca agggaaaaat ccacttggtg agttgttgac ttctgttgcc cttgccgttg
    36601 cagccattcc agaaggactt cctgctatcg ttaccatcgt tctttctctt ggtactcaag
    36661 ttttggccaa acgacattcc atcgttcgta agttgccagc agttgaaaca cttggttcaa
    36721 ctgaaatcat cgcttctgat aagactggta cgctgactat gaacaagatg acagtcgaaa
    36781 aagtcttcta cgatgcggtt ctacatgact cagctgatga tattgaacta ggtcttgaaa
    36841 tgccactact tcgttcagtt gtcttggcca atgatacgaa aatcgatgtg gaaggtaact
    36901 tgattggtga cccaaccgaa acagccttta tccaatatgc cttggacaag ggctatgatg
    36961 tcaaaggttt cttagagaaa tatcctcgtg tagctgaatt gccatttgac tctgaccgta
    37021 agctcatgtc aacagttcac ccattgccag atggtcgttt ccttgtagca gtcaagggtg
    37081 cgccagacca actcttaaaa cgttgtcttc ttcgtgataa ggctggggat attgctccga
    37141 ttgatgagaa ggttacaaat ctcattcata caaacaattc tgaaatggct catcaagcct
    37201 tgcgtgtcct tgcaggtgct tataagatta tcgatagtat tccagaaaat ctcacttctg
    37261 aagagcttga aaatgattta atttttactg gtttgattgg gatgattgac cctgaacgtc
    37321 ctgaagccgc tgaggctgtt cgtgtggcta aggaagcggg aatccgtcca attatgatta
    37381 caggtgacca ccaagacaca gcggaagcca ttgccaaacg tttgggaatc attgacgcaa
    37441 acgatacaga aggtcacgtt ttaactggtg ctgaactcaa tgaactgtca gatgaagaat
    37501 ttgaaaaagt cgttggtcaa tactctgttt atgcccgtgt gtctccagaa cacaaggttc
    37561 gtatcgtcaa ggcttggcaa aaacaaggta aagtcgttgc catgacaggt gacggtgtca
    37621 atgacgcgcc agctctgaaa acagccgata tcggtatcgg tatgggaatc actggtacag
    37681 aggtttctaa gggggcttct gatatgattc ttgcagatga taactttgcg actattatcg
    37741 tcgcagtgga agaaggacgt aaggtcttct caaacattca aaagactatt cagtacctac
    37801 tttctgctaa tactgctgaa gtattaacca tcttcctatc aaccttgttt ggttgggatg
    37861 tcttacagcc ggttcatctt ttgtggatta acttggtaac ggataccttc ccagctatcg
    37921 ctcttggtgt tgaacctgcg gaacctggtg tcatgaatca taaaccacgt ggacgcaagg
    37981 caagcttctt ctcaggtggt gttttgagtt ctatcattta tcaaggtgta ctccaagcag
    38041 ctcttgttat gagtgtttat ggccttgcga ttgcttaccc agttcatgtg ggtgacaatc
    38101 atgctattca tgcagatgcc ctaacgatgg cctttgcaac ccttggtttg attcagctct
    38161 tccatgccta caatgtcaag tctgtctacc aatccatctt gacagttggc ccattcaagt
    38221 ctaagacctt taactggtcc atcttggtat cctttatcct tctcatggca acaatcgttg
    38281 tagaaccgct tgaaggaatc ttccacgtaa ccaaactaga cttgtcacaa tggggaattg
    38341 ttatggctgg aagcttctca atgattatca tcgtcgaaat cgttaagttt attcaacgca
    38401 aactcggttt tgacaagaat gcgatttaag ggaggtttcg gatgtcagca tatcaattac
    38461 cgaccgtatg gcaggatgaa gctagtaatc aaggagcttt tacggggcta aacagaccaa
    38521 cagcaggtgc ccgtttcgaa caaaacttgc caaaaggaga acaagctttt cagctttatt
    38581 cactgggaac accaaatggt gtgaaggtta ctatcttatt ggaagaatta ctagaagctg
    38641 gttttaagga agcggcttac gacttgtata agattgctat catggatggg gatcaattcg
    38701 gctcagactt tgtgaagctc aatccaaatt ccaagattcc agccttattg gaccagtcag
    38761 gtactgaaaa cgtaagagtc tttgagtctg ctcatattct tctttacctt gctgagaaat
    38821 ttggagcctt tttaccaagt aatcctgtgg aaaaggtaga agttttgaat tggctattct
    38881 ggcaagcagg tgcagcacct tttctaggtg ggggatttgg acatttcttc aattatgctc
    38941 ctgaaaaatt ggaatatcct attaaccgtt ttacgatgga agtgaaacgc cagttggatt
    39001 tattggataa ggaattggct cagaaacctt atattgcagg caatgactat acgattgcag
    39061 atattgctat ctggtcttgg tatggacagt tagttcaagg aaatctttac caaggttctg
    39121 caaaattctt ggatgcctca agttatcaaa atctagtaaa atgggcagaa aaaattgcca
    39181 atcgtccagc tgttaagcgt ggcttggaag taacttatac agaaattaaa tagcaaaaaa
    39241 gtcatcttcg gatggctttt tgctacagtt gagggaaagg gtttgcagtt atcggctttt
    39301 gtgctataat tgctataata tagtaaaaag caagaggagt gtgaggaagt ggaaaagaaa
    39361 attgtgaagg atatcttatt tttatctcaa gtgtctcatc cggcaagtca ggaggacctt
    39421 tatcttgcca gagatttgca ggatacactc ttagcaaatc gtgatacctg tgttggtcta
    39481 gctgccaata tgattgggat gcagaagcgc gtgattatct ttaatcttgg cttggttccc
    39541 gtggtcatgt ttaacccagt gcttctgtcc tttgaaggat cctatgaggc agaagaaggc
    39601 tgtttgtcct tggtaggtgt gagatcaact aagcgttatg aaaccataag gcttgcctat
    39661 cgtgacagca agtggcagga acagaccatt accttgacag gcttcccagc tcagatttgc
    39721 cagcatgagc tggatcactt ggaaggacga atcatttagg aggaaagcaa atgaaacgaa
    39781 tagtctttga acttattttt atcgcaacga cctggtatat ctttttaccg ccccttaacc
    39841 tgaccagctg ggaatttctc ttcttcctct gtgggcattt gttagttgtg gcaatcttat
    39901 ttggctttgg caaggggata aaccttgtca aaacggttca tgtgcgccac ggtaaggcgg
    39961 aagctgcctt aaatattgag ggtttcaaaa tcaatcggtt agggaaaatt ctgttagctt
    40021 cgattggagg aattcttctc ttggcagctt tggtttcctt ggtaacttct agcatgtttc
    40081 aggctaaaaa ttatgccaat gtagtcacgg ttacggaaaa agactttact gaatttccta
    40141 agagtgacac cagtaaggtt cctatcctag atagaagtac tgctgaaaaa attggagacc
    40201 gctacttggg ttccctaacc gataaggtgt cgcaatacgt agcggcagat acctataccc
    40261 aattgacaat tgatgggaaa ccttatcggg tcacaccact agaatatgca gaccctatca
    40321 aatggtttaa caatcaagcc aagggaatcg gtgagtatat caaggtggac atggtaactg
    40381 gaaatgcgga tttggtggac ttgaagacac caatcaagta ttcagactcg gagtatttta
    40441 accgtgatgt caaacgtcac ctgcgcttga agtacccgac caaaatcttt aaaactccat
    40501 cttttgaggt ggacgatgag ggcaatcctt tctatgtagc aacggtttac caaaagcaat
    40561 ttggacttgc tgttcctcgt cctgcttcag tcattatctt ggatgctaca aatggagaaa
    40621 ctaaggaata cagcttatca gatgttccag aatgggtgga caggatctat ccagcagagg
    40681 aaaccattga gcaaatcaac tacaacggca agtacaagga cggtttcttg aatgccatga
    40741 tttccaagaa aaacgtgacc cagacgacca atggctataa ttacttgtct atcggtaatg
    40801 acatctatct ctacacaggt gtgacgtcgg ctaatgcgga tgagagtaat cttggtttca
    40861 tccttgaaaa tatgcgaaca ggagaaatta ctaagtatag cttggcttct gcgacagaag
    40921 aatcagcccg tgaatcagca gaaggtgctg ttcaggagaa atcctacaaa gcaaccttcc
    40981 caatcctcat caacctcaat gacaagcctc tctacatcat gggcttaaag gacaatgctg
    41041 gcttggtcaa agagtacgcc ctggtagacg cagtcgagta ccaaaatgtt atcgttgcta
    41101 ctacagtgga agagatgctc agcaagtatg ccaataaaaa cgaccttgaa attgacaatg
    41161 caacgacaga aagcatcaag ggagtagtag cagacctcaa atcagctgtt atcaagggag
    41221 acactgtcta cttctttaaa gttgatggca agatatacaa ggtaaaggct tcagtatccg
    41281 atgaccttcc ttaccttgaa aatggtaaaa ccttcgaagg tcaagtagga aaagacaatt
    41341 atctcaagac ctttaaagta caataatact cttcgaaaat ctctagctct atctgcaacc
    41401 tcaaaacagt gttttgagca gcctgcggct agtttcctag tttgctcttt gattttcatt
    41461 gagtataaaa aaggtttatt taataaatat atgttataat aaggtaaatt aagcctaatg
    41521 gaggacaaag aaatgggttt ttatttgatg gttgcttcaa tgctactcgg actgctcgct
    41581 ttgaagatag gtttttctca gttcaaggag aaaaaagata aattcttatc tatcttaaca
    41641 agtttagctg gcacagccct tgttctcgtg gctgtctggt taggatggcc caaataaata
    41701 gaaaaacctc ggtcaaaccg aggtttttca aatgaatttc ttggttgtgg ctaaaaaata
    41761 tgctacacta tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa cacagacttg
    41821 ccaagtatcg aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga tgccttggct
    41881 ctctattctg ccagtcaatt ggagagtttc tacaaggtat cagctgagaa agcggcggaa
    41941 gaatttcaaa atatccaagc tttgaaaagg caaactgctc aacactatcc agccttgaaa
    42001 ctttttgatg ggcttatgta ccgcaacatt aagagagata agctgaccga ggcggaacaa
    42061 gattatcttg aaaatcatgt cttcattacc tcggctttgt acggtgttgt tccagccttg
    42121 tcacccatgg ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc tggtaagact
    42181 ttgaagagcc attggaaggc agtctatgat gaagctctga agaaggaaga agtgattttc
    42241 tctctcttgt catcagagtt tgagactgta ttttctaagg aaatcagagc aaagatggtg
    42301 accttcaaat tcatggagga tagaggcggt cagctgaaga ttcactcaac tatctccaag
    42361 aaagcgcgcg gggcctttct aacagcttta atagaaaatc aagtacaaac tgtgggggaa
    42421 gcacgtcgct tgaactttgc tggatttgtt taccgagaag atttgtcaca accacaggga
    42481 ttggtttttg taaaggaagt atagaaatca gataagagaa aagtcagcac gattatgctg
    42541 acttttttga taacttataa agtattggac aaatgaatct catttgataa ggtgttaaga
    42601 tgattaacca aattcatgat tgacagatga tttcggcaaa taaacttctt ggtaatccct
    42661 gaaatataga aattagacac gatgatatcg taatctgtct gatttaaaat ctcaggactt
    42721 gtttttaatt catcccaaat atcgaaggta aagcggttat tacagtaata ggaaagcata
    42781 tcaacaaagg taagggatat agcatggtca aagttactga taatcaaaac cttgataggt
    42841 ggtcgatttt ctaacaactg agtagagagg ttttcagcat gggttaagat ggtgtacatc
    42901 aagtgttcca actgttcagg atggtcatgc tgtccaattg cctgacggta ttgagccagt
    42961 tcttgacgcg cacttcccat aaagtctggg aagtaacctt caaatttttt aatcgtcaat
    43021 gctttttgtt caaataagat tggggtagaa aagatttcct gtctttcaaa gaaagcagtg
    43081 ttgtgtaaat gccaaatcag ctcatcgtgg ttagtaaaag taatcttata ttgttttgat
    43141 aggcgttcta atatttgact aagtaattgg taagagtatc ttgcttgact gttatcttct
    43201 aaagagttga agaattcttg aggatctagg aaaatatcat tctgaagaaa agaaataaaa
    43261 atttgagcga ttgtatctgg agtcatttca agcccaaaat gtttagagaa ataagcaagt
    43321 gtctcctgga aatttggaat gctctttaaa agaggataat attctttgta aaagtggttg
    43381 gggaggtcaa tgaagtgacc atttttaacc ctgtggatac tgatagctat catcagtttg
    43441 tacattctat aaattgaaaa tcgcattgga taattcgtga ttttatagaa gaagtcagca
    43501 aactctgtta aatcttcctc aggaaggtca ggaaaaggcc aatctaggaa ataatagcgc
    43561 tcagaaaagt attgggcaaa aaagtaacga atatcgatct catttccacg tatctcggat
    43621 ggagtaaaag ataattcaat ttgaaactga gatgaaagaa cctttgtaat atttctgccc
    43681 agtcgataga ggttggcact actaaagtaa agattttcta ttgttcgata aataggtagc
    43741 ccctcattga agaacatata ctctagtaat tgaaaagatt gagaattagc aagaaaatat
    43801 tgataaatat cttctacact agtgttaact tctaaatcta tcatgattcc gttaacagaa
    43861 gattggatgt taatagatgg aaaggcaata cgtaattcat ttaaatcact ttttaagatt
    43921 cgttctgtac atcctaattt ttcggctagt tcatgtaatt tcatccaatt acgattctga
    43981 attagggttt ccattaagcg taattgtctt tgaacttttg tggataaaag gtttctcata
    44041 cgggtatttc ctccataatt cattatatct ttttttgtct aagtagtata aaaaaattag
    44101 agcaagaaaa ttgtcgttag taatcggaaa aattagaata atataagtgg cttttaagtg
    44161 caaaaacaga atatattaat tgttcagaaa atattgatta atgcctcaaa tagttgtaca
    44221 ttgtaaacta taaaatacta tctaggagtt taaaatgaaa cattcacata aaaaatcatt
    44281 tgactggtat agcatgcaac aacgttattc tattcgtaag tattactttg gtgcagttag
    44341 tgtcttgctc ggtaccgctt tggtattagg tgcagcagct agtgtccaaa cagtacaagc
    44401 ggaagaaaac aaacaagaaa ctaccaatag tatttctgtt ggtaggggag aagcagctac
    44461 taaaccagca gaggtttctg cgtctaataa agagaaaacc tatgcagctc caactgtagc
    44521 taatccagta gaaacgactc cagttaaaac tggagaagtt actaaaccag cagaaaaagt
    44581 tgaagaagca aaagacaaaa aagaggaagt aacgcatcaa gatgctattg acaagtcaaa
    44641 attgttaacg gctctttcgc gtgctaaaaa gttagaaagc aagttatata cagaagcaag
    44701 tgctgctaac ttgcaaacaa gtatccaagc tggtcaaagc ttgcttggga aagcagatgc
    44761 atctgaagct gaattatcag cagcagagtc atctattcaa tcatctatta ttggtctaga
    44821 acttcgttct aactctaaca aaggaactgt atcagaaact cctgtagcga agaaagctaa
    44881 tatagttgaa gcgaaggaag agactaatcc agctgcaaca actgatcgtt cagctgttga
    44941 tagtgctgtt ttgccaacta gcacagctgc caaagtagaa acaacttcag ctccagcatc
    45001 tattaatgaa atcttgaaac caggtttgag cctttctgat gctcgccaaa atccagctat
    45061 ccgtaaggaa gatgttgata gagggtatag tggttttaga gcggcgcctg ctccaacgaa
    45121 ccaaccagta actaatctag gaaataatac tgttttcact gatattagca gaggttctca
    45181 tactttccgt ggtaatggaa attcacgtcg tggaaacccg attcattttg atgtaacaac
    45241 aactagagtt agaaatagag tagatttctc aatcagatat tcaggaccag gtgagtttgt
    45301 taataacaac tttatcttag acaaaggaaa tggattcgga gaaccttctc gtgcaactgt
    45361 ttcaacacca aaattaagag accaagttaa aaacattagt aagggtggaa actttgtttc
    45421 tcattcaggc tacagtttag aagctgcagt agaaacaagc accaatcaaa caattaagtt
    45481 tagtttacct atagccaacc ctaatggtga tctgtctgtt cgattaagac ctgttacttt
    45541 taatgtaggt caaggggaat caggatctgc aactagtaat gatccatata gtaactctaa
    45601 ctattacttc agagaaaaac cactcctctt ggatgcaaat ccaaatggtg gtactaataa
    45661 taagactgtt tcagaagaca ttgacttcca aactgtctat cttccaacta gtaagttacc
    45721 agaaggtcaa actagattag ttcgagaagg tgaaaaagga caacgtcaaa tcatctataa
    45781 agttcatcaa ttgggtaacg aaacaatcct aggattgcca attagtaatt cagttattaa
    45841 agaagttaag ccacgtatta tgcaaattgg tgtggctaaa gatctaatcg atacagtaaa
    45901 accacgtgtt gatcaaaata aagtcggtga tacaaataac ctcactttct atcttgataa
    45961 cgatggaaac ggtgtttata ctgagggcgt agacgaactt gttcaaaaaa ttgctattaa
    46021 agatgg
//
LOCUS       DAVBCR010000008        45502 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCR010000008 DAVBCR010000000
VERSION     DAVBCR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.008"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            434..886
                     /locus_tag="VQ508_000391"
     CDS             434..886
                     /locus_tag="VQ508_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2489447.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VQ508_000392"
     CDS             905..2242
                     /locus_tag="VQ508_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2489448.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VQ508_000393"
     CDS             2321..3205
                     /locus_tag="VQ508_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2489449.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VQ508_000394"
     CDS             3205..4038
                     /locus_tag="VQ508_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2489450.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4056..6149
                     /gene="nanB"
                     /locus_tag="VQ508_000395"
     CDS             4056..6149
                     /gene="nanB"
                     /locus_tag="VQ508_000395"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2489451.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6161..7264
                     /locus_tag="VQ508_000396"
     CDS             6161..7264
                     /locus_tag="VQ508_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2489452.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7438..8136
                     /locus_tag="VQ508_000397"
     CDS             7438..8136
                     /locus_tag="VQ508_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2489453.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8155..9672
                     /locus_tag="VQ508_000398"
     CDS             8155..9672
                     /locus_tag="VQ508_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2489454.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9797..11125
                     /locus_tag="VQ508_000399"
     CDS             9797..11125
                     /locus_tag="VQ508_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2489455.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11377..12243
                     /locus_tag="VQ508_000400"
     CDS             11377..12243
                     /locus_tag="VQ508_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2489456.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12259..13098
                     /locus_tag="VQ508_000401"
     CDS             12259..13098
                     /locus_tag="VQ508_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2489457.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13370..13822
                     /locus_tag="VQ508_000402"
     CDS             13370..13822
                     /locus_tag="VQ508_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2489458.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            13919..14263
                     /locus_tag="VQ508_000403"
     CDS             13919..14263
                     /locus_tag="VQ508_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489459.1"
                     /translation="MVSSKHAARASFLDGQGITVDETSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14547..15191
                     /locus_tag="VQ508_000404"
     CDS             14547..15191
                     /locus_tag="VQ508_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2489460.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVISCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15360..16277
                     /locus_tag="VQ508_000405"
     CDS             15360..16277
                     /locus_tag="VQ508_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2489461.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLITDKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16295..17179
                     /locus_tag="VQ508_000406"
     CDS             16295..17179
                     /locus_tag="VQ508_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2489462.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17195..18046)
                     /locus_tag="VQ508_000407"
     CDS             complement(17195..18046)
                     /locus_tag="VQ508_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2489463.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18246..20303
                     /gene="pbp2b"
                     /locus_tag="VQ508_000408"
     CDS             18246..20303
                     /gene="pbp2b"
                     /locus_tag="VQ508_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2489464.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20314..20910
                     /gene="recR"
                     /locus_tag="VQ508_000409"
     CDS             20314..20910
                     /gene="recR"
                     /locus_tag="VQ508_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2489465.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVATMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21086..22129
                     /locus_tag="VQ508_000410"
     CDS             21086..22129
                     /locus_tag="VQ508_000410"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2489466.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22213..23586
                     /locus_tag="VQ508_000411"
     CDS             22213..23586
                     /locus_tag="VQ508_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2489467.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23573..24184
                     /locus_tag="VQ508_000412"
     CDS             23573..24184
                     /locus_tag="VQ508_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2489468.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24275..24979
                     /locus_tag="VQ508_000413"
     CDS             24275..24979
                     /locus_tag="VQ508_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2489469.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25198..26571
                     /gene="ftsA"
                     /locus_tag="VQ508_000414"
     CDS             25198..26571
                     /gene="ftsA"
                     /locus_tag="VQ508_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2489470.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26588..27847
                     /gene="ftsZ"
                     /locus_tag="VQ508_000415"
     CDS             26588..27847
                     /gene="ftsZ"
                     /locus_tag="VQ508_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2489471.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27852..28523
                     /locus_tag="VQ508_000416"
     CDS             27852..28523
                     /locus_tag="VQ508_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2489472.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28533..29072
                     /locus_tag="VQ508_000417"
     CDS             28533..29072
                     /locus_tag="VQ508_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2489473.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29072..29335
                     /locus_tag="VQ508_000418"
     CDS             29072..29335
                     /locus_tag="VQ508_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2489474.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29332..30117
                     /locus_tag="VQ508_000419"
     CDS             29332..30117
                     /locus_tag="VQ508_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2489475.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30126..30914
                     /gene="divIVA"
                     /locus_tag="VQ508_000420"
     CDS             30126..30914
                     /gene="divIVA"
                     /locus_tag="VQ508_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2489476.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31262..34054
                     /locus_tag="VQ508_000421"
     CDS             31262..34054
                     /locus_tag="VQ508_000421"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2489477.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            34464..34826
                     /locus_tag="VQ508_000422"
     CDS             34464..34826
                     /locus_tag="VQ508_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489478.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            35361..36053
                     /locus_tag="VQ508_000423"
     CDS             35361..36053
                     /locus_tag="VQ508_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2489479.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36106..38517
                     /locus_tag="VQ508_000424"
     CDS             36106..38517
                     /locus_tag="VQ508_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2489480.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            38767..39468
                     /locus_tag="VQ508_000425"
     CDS             38767..39468
                     /locus_tag="VQ508_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489481.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39470..42170
                     /locus_tag="VQ508_000426"
                     /pseudo
     CDS             39470..42170
                     /locus_tag="VQ508_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(42266..42757)
                     /gene="tpx"
                     /locus_tag="VQ508_000427"
     CDS             complement(42266..42757)
                     /gene="tpx"
                     /locus_tag="VQ508_000427"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2489482.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(42880..43809)
                     /gene="psaA"
                     /locus_tag="VQ508_000428"
     CDS             complement(42880..43809)
                     /gene="psaA"
                     /locus_tag="VQ508_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2489483.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(43841..44689)
                     /locus_tag="VQ508_000429"
     CDS             complement(43841..44689)
                     /locus_tag="VQ508_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2489484.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44686..45408)
                     /locus_tag="VQ508_000430"
     CDS             complement(44686..45408)
                     /locus_tag="VQ508_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2489485.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        13844 a         8152 c         9986 g        13520 t
ORIGIN      
        1 ttagaaaaac gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatactgaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgccttgagg ttgtagataa gactgaccta tataatccaa ggtgaagcga
      301 ctgtggtttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccattta ggaacttatg tgggagtaaa
      481 tccacatttt gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggggctttct ttgaaacaca ccaaaaatat ttggatattc atttagtttt
      661 ggaaaacgaa gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata
      721 tgatgaagag aaagatattg aattatacac agggaaagtg gaacagttgg ttcatttgag
      781 agctggtgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggttcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaatt ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaagt ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaacga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgatctga
     2281 aaagctctag cccaattttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttctct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gctggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtactgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcagttt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca acccttattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct acattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata agattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa
     4081 aactaggaat ttccgttgta ggcattagtc ttttaatggg agtccccact ttgattcatg
     4141 cgaatgaatt aaactatggt caactgtcca tatctcctat ttttcaagga ggttcatatc
     4201 aactgaacaa taagagtata gatatcagct ctttgttatt agataaattg tctggagaga
     4261 gtcagacagt agtaatgaaa tttaaagcag ataaaccaaa ctctcttcaa gctttgtttg
     4321 gcctatctaa tagtaaagca ggctttaaaa ataattactt ttcaattttc atgagagatt
     4381 ctggtgagat aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcca
     4441 gaccagcttc attatgggga aaacataaag gacaggcagt tgaaaataca ctagtatttg
     4501 tatctgattc taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg
     4561 aaacagttga tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag
     4621 gagctgttaa tcgtgaaggt aaggaacatt acctcgcaaa aggaagtatt gatgaaatca
     4681 gtctatttaa caaagcaatt agtgatcagg aagtttcaac tattcccttg tcaaatccat
     4741 ttcagttaat tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac
     4801 tatatacatt aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc
     4861 atgattctaa aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt
     4921 ggagtgagcc aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc
     4981 cacgagataa taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat
     5041 ccattgttga agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg
     5101 gtattggaaa taataatgca aataaagccg actcaggttt taaagaaata aatggtcatt
     5161 attatttaaa actaaagaag aatggagata acgatttccg ttatacagtt agagaaaatg
     5221 gtgtcgttta taatgaaaca actaataaac ctacaaatta tactataaat gataagtatg
     5281 aagttttgga gggaggaaag tctttaacag tcgaacaata ttcggttgat tttgatagtg
     5341 gctctttaag agaaaggcat aatggaaaac aggttcctat gaatgttttc tacaaagatt
     5401 cgttatttaa agtgactcct actaattata tagcaatgac aactagtcag aatagaggag
     5461 agagttggga acaatttaag ttgttgcctc cgttcttagg agaaaaacat aatggaactt
     5521 acttatgtcc cggacaaggt ttagcattaa aatcaagtaa cagattgatt tttgcaacat
     5581 atactagtgg agaactaacc tatctcattt ctgatgatag tggtcaaaca tggaagaaat
     5641 cctcagcttc aattccgttt aaaaatgcaa cagcagaagc acaaatggtt gaactgagag
     5701 atggtgtgat tagaacattc tttagaacca ctacaggtaa gatagcttat atgactagta
     5761 gagattctgg agaaacatgg tcgaaagttt cgtatattga tggaatccaa caaacttcat
     5821 atggcacaca agtatctgca attaaatact ctcaattaat tgatggaaaa gaagcagtca
     5881 ttttgagtac accaaattct agaagtggcc gtaagggagg tcaattagtt gtcggtttag
     5941 tcaataaaga agatgatagt attgattgga aataccacta tgatattgat ttgccttcgt
     6001 atggttatgc ctattctgcg attacagaat tgccaaatca tcacataggt gtactgtttg
     6061 aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag caatgtagtt cagtatatag
     6121 atttggaaat taatgattta acaaaataaa ggagaaaaac atggttaaat acggtgttgt
     6181 tggagcaggg tattttggag ctgaattggc tcgctacatg caaaagaatg atggagcaga
     6241 gattactctt ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggggcaaa
     6301 agtagcaagt tccttagatg agttggtttc tagcgatgaa gtagattgtg ttatcgtcgc
     6361 aactccaaat aatcttcata aggaaccggt tattaaggct gcacagcatg gtaaaaatgt
     6421 tttctgtgaa aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcgtg
     6481 taaagaaaac aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca
     6541 tcatgcaaaa gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc
     6601 tcgtaatggt tgggaagaac aacaaccgtc agtatcatgg aaaaaaattc gtgaaaaatc
     6661 aggtggtcac ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg
     6721 catgcctgaa actgtaacca tgacaggtgg aaatgtggcc catgaaggtg aacatttcgg
     6781 tgatgaagat gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt
     6841 agaatggggt tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg
     6901 tgccatccgc ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag
     6961 ctatttcttg attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag
     7021 tacagagatg gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct
     7081 atcatctgtc attgataaag aaatgcgcta tctgcatgag attatggaag gagctccagt
     7141 atcagaagaa tttgcaaaac ttttgacagg tgaagctgct ctagaagcaa ttgctactgc
     7201 agatgcttgt acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa
     7261 ataaattttg gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag
     7321 gagctgtttg actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa
     7381 gcgttttaat tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg
     7441 ccacagatta gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt
     7501 caggctcttc ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc
     7561 aaagcggctg agcaaggtgg agcagtcggt atccgagcaa acagtgttcg cgatatcaag
     7621 gaaattaagg aggtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct
     7681 caggaaccct tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc
     7741 gaggtgattg ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag
     7801 ttcattcgtc aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc
     7861 ttcgaagaag ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca
     7921 ggctacacat cctacagtcc aaaagtagac ggtccagatt ttgaattgat taagaaactc
     7981 tgtgatgccg gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa
     8041 caaatccttg aatatggagt gcgaggcatc gttgttggtg gcgccattac tagaccaaaa
     8101 gagattacag aacgcttcgt tgctagtctt aaataagatg tgagggggag ttttatgttt
     8161 aaagttttac aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca
     8221 ggtctacttt tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca
     8281 atactagaca atagtatttt tcaatcaata ttccaagtaa tgagctctgc aggagaggtt
     8341 gtattcagta atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga
     8401 gataaaggaa ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc
     8461 aaagctttgg taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga
     8521 gcattagttg tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta
     8581 ccttccgctt taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct
     8641 tctatcttga ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt
     8701 tctacaggtg gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta
     8761 atgagacttt ctggagcagt aggcttacat catataattt accctatgtt ttggtatact
     8821 gaacttggtg gtgttgaaac tgttgcagga caaacagtgg ttggagctca aaaaatattt
     8881 tttgctcaat tagccgattt ggcccattct ggattattta cagaaggaac aaggtttttt
     8941 gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat
     9001 agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt tttttggagt tgctttaaca
     9061 tcttttatta ccggtattac agaaccaatt gaatttatgt ttctattcgt cagtccggtt
     9121 ctatatgttg ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat
     9181 atttcaatag gaaacacatt ttcaggaggt gtaatcgatt tcactttatt tggaattttg
     9241 caggggaacg ctaagacgaa ttgggttctt cagattccat ttggacttat ttggagtgtt
     9301 ttgtattata ttatttttag atggttcatt actcaattca acgttctaac gccagggcga
     9361 ggagaagaag tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca
     9421 gattatttaa aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata
     9481 gaagatgtag atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt
     9541 gataaagcac ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggcatt
     9601 caagcaatct atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt
     9661 gtagatgatt aagtacttac tgacttaata aaaaacagag gagagtgatg gatgagtagg
     9721 atgaaatgaa atcgcataca agaaataaag aactcattat ccaagttgga tacgcttatt
     9781 acataggaga atacaaatga aatttagaaa attagcttgt acagtacttg cgggtgctgc
     9841 ggttcttggt cttgctgctt gtggcaattc tggcggaagt aaagatgctg ccaaatcagg
     9901 tggtgacggt gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa
     9961 aactggtgac ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagcaaa
    10021 cccagatata aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac
    10081 aacagccatc gaagcaggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat
    10141 ccaatacggt aaaaacggta aattggctga gttgaatgac ctcttcacag atgaatttgt
    10201 taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttatat
    10261 gtatccgatt agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc
    10321 tggagtagca aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa
    10381 agcacttaaa gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga
    10441 ccaaggaaca cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt
    10501 tagcaaatat acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactagctg
    10561 gattaaagac aatttgatca ataatggttc acaatttgac ggtggggcag atatccaaaa
    10621 ctttgccaac ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca
    10681 agctaaactt ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga
    10741 aggtaagcca gctcttgagt accttgtaaa cgggtttgca gtattcaaca ataaagacga
    10801 caagaaagtc gctgcatcta agaaattcat ccagtttatc gcagatgaca aggagtgggg
    10861 acctaaagac gtagttcgta caggtgcttt cccagtccgt acttcatttg gaaaacttta
    10921 tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa tactactcac catactacaa
    10981 cactattgat ggatttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc
    11041 aaatggtgac gaaaaaccag cagatgcttt gaaagccttc actgaaaaag cgaacgaaac
    11101 aatcaaaaaa gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag
    11161 aacctaagag tgtatacccc cttttccctc tacacagata gtgtaagaaa agggggcttt
    11221 tgtttaaaat gtaagaaact gtcacgaaat taaaatgaag ttcttacata agcgaatcat
    11281 aaaaaatttc attttgattt taaaacagtt caagaaagtc aaaaaattat tctatttgaa
    11341 agagaggtgc cgactgtgaa agtcaataaa atccgtatgc gggaaacagt gatttcctac
    11401 gctttcctag caccagtatt attcttcttt gtcatctttg tgttggctcc gatggtgatg
    11461 ggcttcatta caagtttctt taactactca atgactaaat ttgagtttgt aggcttggat
    11521 aactatatcc gtatgtttaa agatcctgtc tttacaaaat ctctgattaa cacagttatt
    11581 ttggttattg gatctgtacc agttgttgtt ctattctcac tctttgtagc atctcagacc
    11641 tatcatcaaa atgtcattgc cagatccttc taccgtttcg tcttcttcct tcctgttgta
    11701 acgggtagtg ttgccgtgac agttgtttgg aaatggattt atgacccact atcagggatt
    11761 ctaaactttg tccttaagtc cagccacatc atcagccaaa acatttcttg gttgggagat
    11821 aaaaactggg cattgatggc gattatgatt attctcttga ccacttcagt tggtcagccc
    11881 atcatccttt atatcgctgc catggggaat attgacaatt cactggttga agcggcgcgt
    11941 gttgatggtg caactgagtt tcaagttttt tggaagatta aatggccaag ccttcttcca
    12001 acaactcttt atattgcaat catcacaaca attaactcat tccagtgttt cgccttgatt
    12061 cagcttttga catctggtgg tccaaactac tcaacaagta ccttgatgta ctacctttac
    12121 gaaaaagcct tccaattgac agaatacggc tatgccaaca caattggtgt cttcttggca
    12181 gtcatgattg ctatcgtaag ctttgttcaa tttaaagtac ttggaaacga cgtagaatac
    12241 taaagaaagg agacagctat gcaatctaca gaaaaaaaac cattaacagc ctttactgtt
    12301 atttcaacaa tcattttgct cttgttgact gtgctgttca tctttccatt ctactggatt
    12361 ttgacagggg cattcaaatc acaacctgat acaattgtta ttcctcctca gtggttccct
    12421 aaaatgccaa ccatggaaaa cttccaacaa ctcatggtgc agaaccctgc cttgcaatgg
    12481 atgtggaact cagtatttat ctcattggta accatgttct tagtttgtgc aacctcatct
    12541 ctagcaggtt atgtattggc taaaaaacgt ttctatggtc aacgcattct atttgctatc
    12601 tttatcgctg ctatggcgct tccaaaacaa gttgtccttg taccattggt acgtatcgtc
    12661 aacttcatgg gaatccatga tactctctgg gcagttatct tgcctttgat tggatggcca
    12721 ttcggtgtct tcctcatgaa acagttcagt gaaaatatcc ctacagagtt gcttgaatca
    12781 gctaaaatcg acggttgtgg tgagattcgt accttctgga gtgtagcctt cccgattgtg
    12841 aaaccagggt ttgcagccct tgcaatcttt accttcatca atacttggaa tgactacttc
    12901 atgcaattgg taatgttgac ttcacgtaac aatttgacca tctcacttgg ggttgcgacc
    12961 atgcaggctg aaatggcaac caactatggt ttgattatgg caggagctgc ccttgctgct
    13021 gttccaatcg tcacagtctt cctagtcttc caaaaatcct tcacacaggg tattactatg
    13081 ggagcggtca aaggataata ctctgcgaaa atctcttcaa actacgtcag cgtcaccttg
    13141 ccatacttaa gtattgcctg cggttagctt cctagtttgt tcttcaattt tcattgagta
    13201 taggaaaatc aatctatcaa gatacagaag tatattttat agatttagag aatatagagg
    13261 ttataagtgt ctacaaaatg gagggtatgc agttacttta tgaagttttg tcagacactt
    13321 ataaacttaa gaatggtttt agttaactat cagaaacgaa ggaaagagta tgatttttga
    13381 cgatttgaaa aacatcacct tttacaaagg gattcatcct aatttagaca aggctatcga
    13441 ctatctctac caacatcgta aggattcttt cgaattagga aagtatgaga ttgatggaga
    13501 taaagtcttt ctagttgttc aggaaaatgt cctcaatcaa gctgaaaatg atcaatttga
    13561 gtatcataag aactatgcag atttgcattt gctggtagaa ggacatgaat attcgagcta
    13621 cggttcacgt atcaaagacg aggcagtagc attcgacgaa gcgagtgaca ttggctttgt
    13681 tcattgtcat gaacactacc cactcttgtt gggttatcac aattttgcga ttttcttccc
    13741 aggtgagcca catcagccaa atggttatgc aggcatggaa gaaaaggttc gaaaatatct
    13801 ctttaaaatt ttgattgatt aaaaatagga tgaattgttt ttttgtaaag ctttgacaat
    13861 actctaccat gaaattgatc tttgtgaggt agagaaatga gaataaaata tttaaaaatt
    13921 ggtatcttct aagcatgctg caagagctag tttcttagat ggacagggaa ttacagttga
    13981 tgagacgtcc tggataatta atggcattgt gaatgcattg attggtagat acataaaatt
    14041 aggtacttat gcagctaagt atggtattag tatagcacgc tcgatcttaa gtagggcagc
    14101 tgcaactgca gcagcaagag taggattact gaccaagatt tctggatgga ttttacgagt
    14161 agctgtgaat gtagctgatg tatatggtaa ttttgccaac aatattgctg cagcttggga
    14221 tgcatatgat aaaattccta acaatggtcg tataaacttt taaaatgcga gaatgaaagc
    14281 actttgtatt tttttattga atatgttagc ttggacagtg cttgcaatga taattcgtgg
    14341 agggctagat ggatttgatg agcatacttg gagtactatt ttaattgctt cgctgttcgg
    14401 ggtatatgat tataagccca tagataaaaa tagaaaaaag tccaaaagaa aaaagaaaaa
    14461 atagatttgt tcatggtagg gacttatgaa agctttactg acaaaaaaga aaacagttta
    14521 caaagaaaaa tgatggagga gcaaacatgg cacaaaaagg agtaagcctt atcaaggcag
    14581 catttgatac agataacttt ctcatgcgtt ttagtgagaa agtcttggac atcgtgacag
    14641 ccaatcttct ttttgtcatc tcttgtttgc ccatcgtgac gattggagtg gctaaaatca
    14701 gcctctacga gaccatgttc gaagttaaga agagcagacg ggtgcctgtt tttaaaatct
    14761 atctaagatc tttcaagcaa aatctgaaac taggtcttca gctgggttta atggagttag
    14821 gaattgtgtt tcttaccctt tcagacctct atcttttctg gggtcaaaca gctctgccct
    14881 tccaattgct gaaagccatt tgtttaggta ttctgatttt tcttactatc gtgatgctgg
    14941 ctagttaccc tatcgcggca cgttatgacc tatcttggaa agaaattctt caaaaaggat
    15001 tgatgttggc tagttttaac tttccttggt tcttcctcat gttagccatt cttgtcctca
    15061 ttgtgatggt tctttatctg tccgccttca gtctactctt aggtggctca gtcttcctac
    15121 tttttgggtt tggactattg gtctttatcc agactggatt gatggagaaa attttcgcaa
    15181 aataccaata ggagccttat ttctgaaact actttcaaag gttccaaacg ctattctata
    15241 agcgagaaac taaaatctaa gtgtctataa gatattgaaa gccttttcgg taaagtgtat
    15301 actaaagaag taaagaaaaa tctgctttag cagaaaataa aaataatagg agaaaatcta
    15361 tgtcagattt gaaaaaatac gaaggtgtca ttccagcctt ctacgcatgt tatgatgatc
    15421 aaggagaagt aagcccagaa cgtacgcgtg ccttggttca atacttcatt gataaaggtg
    15481 ttcaaggtct ttatgtcaat ggttcttctg gtgaatgtat ctaccaaagc gttgaagatc
    15541 gcaagttgat tttggaagaa gttatggcgg ttgccaaagg taaattgacc attattgccc
    15601 atgttgcctg caacaatacc aaggatagta tggaacttgc tcgccatgct gaaagcttgg
    15661 gagtagatgc tattgcaacg attccaccaa tttatttccg cttgccagaa tactcagttg
    15721 ccaaatactg gaacgatatc agttctgcag ctccaaatac agactacgtg atttataaca
    15781 ttcctcaatt ggcaggtgtt gctttgactc caagccttta cactgaaatg ttgaaaaatc
    15841 ctcgtgttat cggtgtgaag aactcttcta tgccagttca agatattcaa acctttgtga
    15901 gccttggtgg ggaagaccat atcgtcttta acggtccaga tgaacagttc ctaggtggtc
    15961 gcctcatggg tgccaaagct ggtatcggtg gtacttatgg tgctatgcca gaactcttct
    16021 tgaaactcaa tcagttgatt acggataagg acctagaaac agcgcgtgaa ttgcagtatg
    16081 ctatcaacgc aatcattggt aaactcactt ctgctcatgg aaatatgtac ggtgtcatca
    16141 aagaagtctt gaaaatcaat gaaggcttga atattggatc tgttcgttca ccattgacac
    16201 cagtgactga agaagatcgt ccagttgtag aagcggctgc tgccttgatt cgtgaaacca
    16261 aggagcgctt cctctaatct aaaaggaggt atttatgaca tattacgttg caattgatat
    16321 cggtggaacc aacatcaagt atggtttggt tgatcaagag gggcaacttc ttgaatcgca
    16381 tgaaatgcca actgaggcgc ataagggtgg acctcatatc ttacaaaaga ccaaagatat
    16441 cgtagctagt tatttagaaa aaggcccagt agcaggtgtt gccatatctt ctgctgggat
    16501 ggtggatccg gataagggtg agattttcta tgctgggccg caaatcccta actacgcagg
    16561 cacccagttc aaaaaggaaa tcgaagaaag ctttactatt ccttgtgaga ttgaaaatga
    16621 tgtcaactgt gcaggtcttg ctgaggcagt atctggttca ggcaagggag caagtgtgac
    16681 actttgcttg accattggaa caggtatcgg tggttgcttg attatggata ggaaagtctt
    16741 ccatggtttt agcaattcag cctgtgaagt cgggtatatg catatgcagg atggagcttt
    16801 tcaagacttg gcttctacaa cagctttagt gaaatatgta gctgaagccc atggagaaga
    16861 tgttgatcag tggaatggcc gtagaatttt caaagaagcc actgaaggaa acaaaatctg
    16921 catggaaggt attgaccgta tggttgacta tctaggaaaa ggtctggcaa atatttgcta
    16981 cgttgccaat ccagaagtgg tcattcttgg tggtggtatc atggggcaag aggctatcct
    17041 caaacctaag atccgcacag ccttgaaaga ggctttggta ccaagtttag cagaaaaaac
    17101 acgattagaa tttgcccatc accaaaatac agcagggatg ttgggtgcat attatcattt
    17161 taagacaaaa caatcctagt ttggctcagc caaactagga ttttcttaca cgtttttgtc
    17221 tacgatagcc gttgagtttt ttattttccc agtagctatt aaagattttt tccttgcttt
    17281 cgcgattgat ttccaaaaag taggcataaa tcaaatcgat aaagaagagc ataggaagtt
    17341 gagcggatat tcgttggatg taggagggtt ggctgtgggt ggctacaaga acagtctctg
    17401 tataggtctg gctattttta ttgggaacac ttgtaaagag tacagtcttt gcccccatct
    17461 ccttggcatc taatagacta tctaaaatag aaggagttga gcctgaaagt gagaagccca
    17521 gtactagaca attttcatcc atgatgctgg ttgtccaggc aaagccgtct tggtctgtca
    17581 aagcttcgca gaccacacct aatcgcataa aacgtaattt catttcacgg gcgacgaggc
    17641 cagaactccc tgttccaaag aagtagatac gctcagcatc ttcgattagc tgggcaattc
    17701 gctctagttg gatttcgtca atcaagtctt gtgtttgttc cctcatattg ctataacttc
    17761 tgaggactcg tttggtcagt ggactgtgct tggagacttg gttggcttga ttttctgcct
    17821 gatgttggta ttggaaaata aattctcggt agccagtaaa gccacacttt ttagcaaagc
    17881 gggtcaaagc agcttgagaa atatgtaatt tttgggtgac ttgttgagaa gataaatcat
    17941 ctgtaatcgt ttcagcttgc aaaaaatagc gagcgatttc ttgctctagg tctgtcattt
    18001 cttcaaaatg tgaatcaatg atagttgcga tatctggttt gtccataggg aaagctcctt
    18061 tacatgagtc atactggaag actagatcag ggaatagtca cacttcatta taacacataa
    18121 tataaggata gataaataaa aacgcatctc tgttttaaaa acgaaaaaat cgaaaaagct
    18181 tctctctttt ccataatttt ctactcaaat tgtggtacaa ttaagagtaa gattttaagt
    18241 tagaaatgag actgatttgt atgagaaaat ttaacagcca ttcgattccg attcggctta
    18301 atttattgtt ttcaatcgtc attttactct ttatgaccat tattggtcgt ttgttgtata
    18361 tgcaggtttt gaacaaggat ttttacgaaa aaaagctagc ctcagctagt cagaccaaga
    18421 ttacaagcag ttcagcccgt ggggaaattt atgatgctag tggaaaacct ttggtagaaa
    18481 atacgttaaa gcaggttgtt tcctttacgc gtagcaataa aatgacggct acagacttaa
    18541 aagaaacagc taaaaagtta ctgacttatg tgagcatcag ttctccaaat ttgacagaac
    18601 gccagctggc ggattactat ttggctgatc ctgaaatcta taaaaaaata gtggaagctc
    18661 tcccaagtga gaaacgcttg gattcagatg gcaatcgtct atccgaatca gaactgtata
    18721 acaatgcggt cgatagtgta caaacgagtc aactaaacta tacagaggat gaaaagaaag
    18781 aaatctatct ttttagtcag ttaaatgctg ttggaaactt tgcgacagga accattgcga
    18841 cagatcctct aaatgattct caggtggctg ttattgcctc tatttcaaag gagatgcctg
    18901 gcattagtat ttctacttct tgggatagaa aggttttgga aacttccctt tcttctatag
    18961 ttgggagtgt atccagtgaa aaagctggtc tcccagcgga agaagcagaa gcctatctta
    19021 aaaaaggcta ttctctaaat gatcgtgtag gaacctccta tttggaaaag caatatgaag
    19081 agaccttaca aggaaaacgc tcggtaaaag aaatccatct ggataaatat ggcaatatgg
    19141 aaagcgtgga tacaattgag gaaggtagta agggaaacaa tatcaaactg accattgatt
    19201 tggccttcca agatagcgtg gatgctttac tgaaaagtta tttcaattcc gagctagaaa
    19261 atggtggagc caagtattct gaaggtgtct atgcagtcgc ccttaaccca aaaacaggtg
    19321 cggttttgtc tatgtcaggg attaaacatg acttgaaaac gggagagttg acgcctgatt
    19381 ccttgggaac ggtaaccaat gtctttgttc caggttcggt tgtcaaggcg gcgaccatca
    19441 gctcaggttg ggaaaatgga gtcttgtcag gaaaccagac cttgacagac cagtccattg
    19501 tcttccaagg ttcagctccc atcaattctt ggtatactca ggcttacggt tcattcccta
    19561 tcacagcggt ccaagctctg gagtattcat ctaataccta tatggtccaa acagccttag
    19621 gtcttatggg gcaaacctat caacccaata tgtttgtcgg caccagcaat ctagagtctg
    19681 ctatggagaa actgcgttca acctttggcg aatatggctt gggtactgcg acaggaattg
    19741 acctaccaga tgaatctact ggatttgttc ccaaagagta tagctttgct aattacatta
    19801 ctaatgcctt tgggcagttt gataactata cgccgatgca gttggctcag tatgtagcaa
    19861 ctattgcaaa taatggtgtt cgtgtggctc ctcgtattgt tgaaggcatt tatggtaata
    19921 atgataaggg aggactgggt gacttgattc agcaactgca accgacagag atgaataagg
    19981 tcaatatatc cgactccgat atgagcatct tgcaccaagg tttttatcag gttgcccatg
    20041 gtactagtgg attgacaact ggacgtgcct tttcaaatgg tgccttggta tccattagcg
    20101 gaaaaacagg tacagccgaa agctatgtgg cagatggtca gcaagcaacc aataccaatg
    20161 cggtggccta tgccccatct gataatcccc aaatcgctgt cgcagtggtc tttcctcata
    20221 ataccaatct aacaaatggt gtaggacctt ccattgcgcg tgacattatc aatctgtatc
    20281 aaaaatacca tccaatgaac tagaaaggaa attatgcttt atccaacacc tattgccaag
    20341 ttgattgaca gttattctaa gttaccaggt atcgggatta agacggctac gcgtctggcc
    20401 ttttatacga ttgggatgtc tgctgatgat gtcaatgaat ttgcaaaaaa tctcctttct
    20461 gctaagagag aattgacata ttgttctatt tgtggacgtt tgacagacga cgatccttgt
    20521 tctatctgta ccgatccgac tcgtgaccag acaacaattt tagttcttga ggatagtaga
    20581 gatgtggcaa ccatggaaaa tatccaagaa taccatggcc tctatcatgt cctgcatggc
    20641 ctcatttctc ctatgaatgg tatcagtccg gacgatatca atctcaagag ccttatgact
    20701 cgtcttatgg atagtgaagt ttcagaagtg attgtggcga ctaatgctac agcggatggt
    20761 gaagcgactt ccatgtatct ttcacgtttg ctcaagccgg ctggtatcaa ggttacgcgt
    20821 ctagcacgag gtctcgctgt gggagcggac attgagtatg cggacgaagt gacactctta
    20881 cgagccattg aaaatcggac agagttgtaa gtgtaggcaa atttacgaac tccattcatt
    20941 tataaaaaat caaagaggct gaaaatcgtt cctatcggcc tctttttgta tagtgtgatg
    21001 agtaggatcg ggttcaagtt ttaaaaaacc aagcaaatat gatatactaa agagcgagta
    21061 ttctagtaga attaggacaa ataatatgaa acaaacgatt attcttttat atggtggacg
    21121 gagtgcggaa cgcgaagtct ctgtcctttc agctgagagt gtcatgcgtg cggtcaatta
    21181 cgaccgtttc acagtcaaga ctttctttat cagtcagtca ggtgacttta tcaaaacaca
    21241 ggaatttagt catgctccgg ggcaagaaga ccgtctcatg accaatgaaa ccattgattg
    21301 ggataagaaa gttgcaccaa gtgctatcta cgaagaaggt gcagtggtct ttccagtcct
    21361 tcacgggcca atgggagaag atggctctgt tcaaggattc ttggaagttt tgaaaatgcc
    21421 ttacgttggt tgcaacattt tgtcatcaag tcttgccatg gataaaatca cgactaagcg
    21481 tgttctggaa tctgctggta ttgcccaagt tccttatgtg gctatcgttg aaggcgatga
    21541 tgtgactgct aaaatcgctg aagtggaaga aaaattggct tatccagtct tcactaagcc
    21601 gtcaaacatg gggtctagtg tcggtatttc taagtctgaa aaccaagaag aactccgtca
    21661 agccttaaaa cttgccttcc gatatgacag ccgtgtcttg gttgagcaag gagtgaatgc
    21721 ccgtgaaatt gaggttggcc tcttgggtaa ctacgatgtc aagagcacgc tacctggaga
    21781 agttgtcaag gacgttgcct tttatgacta cgatgccaag tatattgata acaagattac
    21841 tatggatatt cctgccaaaa tcagtgatga tgtggtagct gtcatgcgtc aaaatgcaga
    21901 aacagccttc cgtgccattg gtggccttgg tctatctcgt tgcgatttct tctatacaga
    21961 taagggagag atttttctca acgagctcaa taccatgcca ggtttcaccc agtggtctat
    22021 gtacccacta ctttgggaca atatggggat cagctaccca gaactaatcg agcgtttggt
    22081 tgaccttgcc aaggaaagtt ttgacaagcg cgaagcgcat ttgatataaa atgaaagaga
    22141 gggtagaagc cagaaccatc actgcaaggt gactagagtt ctcggacttc agcccttttt
    22201 aaaggagtag aaatgaaatt aacaatccat gaagttgccc aagttgttgg agccaaaaat
    22261 gatatcagta tctttgagga cacccagtta gaaaaagctg agtttgatag tcgtttgatt
    22321 ggaactggag atttatttgt gcccctaaaa ggtgtgcgtg acggtcatga ctttatcgaa
    22381 agagcttttg aaaatggtgc ggcagcaacc ctgtctgaga aagaggttgc aaatcatccc
    22441 tacattctag tagatgatgt tttgactgcc tttcaacaac tagctgccta ctatcttgaa
    22501 aaaacagctg ttgatgtctt tgcggttacg ggttcaaatg gcaagacaac gactaaggat
    22561 atgttggcgc atttactgtc aacaagatac aagacctaca aaacacaagg caattacaat
    22621 aatgagattg gccttcctta cacagttctc cacatgcctg aaggaacaga aaagttggtc
    22681 ttggagatgg ggcaggacca cttgggagat attcatctct tgtctgaatt ggctcgtcca
    22741 aaaacagcca tcgtgacctt ggttggagaa gctcatttgg cctttttcaa agatcgttct
    22801 gagattgcta aaggaaaaat gcaaattgca gacggaatgg cttcaggttc cttgctttta
    22861 gcgccggctg accctatcgt agaggactat ttgccaactg ataaaaaggt ggttcgtttt
    22921 ggtcaaggag cagagctgga aattactgac ttggttgagc gcaaagatag tctgaccttc
    22981 aaggccaatt tcttagagca agtccttgat ttgccagtaa ctggcaagta caatgcgaca
    23041 aatgctatga ttgcatccta tgttgccttg caagaaggag tttcagagga gcaaattcgt
    23101 ttggccttcc aacatcttga attgacgcgt aaccgtaccg agtggaagaa agcagccaat
    23161 ggagcagaca tcttgtcaga tgtttacaat gccaatccaa ctgctatgaa gctgatttta
    23221 gagaccttct ctgccattcc agccaatgaa ggtggcaaga aaattgcagt gttggcggat
    23281 atgaaggaac ttggtgtcca gtctgttcaa cttcataatc agatgatttt gagcctttcg
    23341 ccagatgtgc ttgatactgt tattttctac ggagaagata ttgctgaatt agcccaactt
    23401 gctagtcaaa tgttcccaat cggacacgtt tattacttca agaaaacaga agaccaagac
    23461 caatttgaag acctagttaa gcaggtcaaa gaaagccttg gggctcatga ccaaatcctg
    23521 ctcaaaggct ctaactctat gaatctagcc aagttggtag aaagtttaga aaatgaagac
    23581 aagtgatttt gtcaagtatt tgcaaagaat gattgccatt acagatactg gattaacctt
    23641 tacaaaagat ccgtttgacc gtgagcgcta cgaagacttg cgagaccttc tatctgaaat
    23701 gttgaatcaa gcatcagacc ttgattccga agaagtggca gaagtcttga agccaacttc
    23761 tgcttatgcg actcccttaa tggacgtccg tgcttggatt gttgaggacg aaaagatttg
    23821 tctggttagg ggacaaggag aggatagttg ggctttgcca ggtggctttg gtgaagtcgg
    23881 ctattctcca actgaaaata ttctcaagga aattgaagaa gaaaccggtt ttaaagccaa
    23941 agttgaaaga ctgctagctg tttttgatac caatcgtttc caactacaga gcaaacaata
    24001 tgcaaagttt gtttttgaat gtaagcttct cgatggacaa ttccaagaaa atcaagaaat
    24061 cgctgacctt caattttttg caattgaccc actgccgaat ttatctgaaa aacgcattac
    24121 caaggagcaa atcgagattc tttggcaggt ttatcaaggt cagagggagc aatatcttga
    24181 ctaagaagat gattatcgta tttctaaatt catttttctt aagtagcatg gtataataaa
    24241 atgcaggaaa attttgaatc atgaggaaga ctagatgaat ttatgggata ttttctttac
    24301 gactcaggca accgagccgc ccaaatttga ccttttttgg tatgttagcc tatttacgct
    24361 cttagcctta accttttata cagcccatcg ctatcgtgaa aagaaggttt accaacgatt
    24421 tttccaaatc ttgcagactg ttcagttaat ccttctttat ggttggtact gggtcaatca
    24481 tatgccactg tcagaaagcc taccctttta ccattgccgt atggctatgt ttgtggtact
    24541 cttgcttcct ggtcagtcca aatataaaca atactttgca ttattgggaa catttgggac
    24601 attagcagcc tttgtttatc cagtgccaga tgcttaccct tttccacata tcaccattct
    24661 atcctttatc tttggtcatt tagcactctt ggggaactct ctagtttatc tattgagaca
    24721 gtataatgcg cgattgctgg atgtgaaggg aatttttctc atgacctttg ccctaaatgc
    24781 cttgattttt gtggtcaatt tggtgacagg tggcgattac ggatttttga caaaaccgcc
    24841 attggttggg gatcacggtc tagtagctaa ttatttactt gtttcaattg tgctggtagc
    24901 tactatcagt ttgactaaga aaatcttaga attcttttta gctcaagaag cagaaaaaat
    24961 gattgcaaag gaagcttaac acagagcttt cttttttgct cttagagagt ttttacaagc
    25021 agcttataaa ataagaattt ctgaatagac aaactcaaaa aatggctggg aaatttagga
    25081 aaaaagcaag cacgattaaa ttttttgtgt tataatattt tgtgaatagc tatgcctatg
    25141 tttagctatg gaataatacg aagtgcgaaa cttggaagat agagaggaag cgatgtaatg
    25201 gctagagaag gcttttttac aggtctagat attggaacaa gctctgtcaa ggtgcttgtg
    25261 gccgagcaga gaaatggtga attaaatgta attggcgtga gtaatgccaa aagtaaaggt
    25321 gtaaaggatg gaattattgt tgatattgat gcagcagcaa ctgctatcaa gtcagccatt
    25381 tcccaagcgg aagaaaaggc aggcatttcg attaaatcag taaatgtcgg cttgcctggt
    25441 aatcttttgc aggtagaacc aactcagggg atgattccag taacatctga tactaaggaa
    25501 attacggatc aagatgttga aaatgttgtc aaatcagctt tgacaaagag tatgacacct
    25561 gaccgtgaag tcattacctt tattcctgaa gaatttattg tggatggttt ccaagggatt
    25621 cgtgacccac gtggcatgat gggggttcgc cttgaaatgc gtggtttgct ttatacagga
    25681 cctcgtacta tcttgcacaa tttgcgtaag acggttgagc gtgcaggtgt tcaggttgaa
    25741 aatattatca tttcaccact agcaatggtt cagtctgttt tgaatgaagg ggaacgtgaa
    25801 tttggtgcta cagtgattga tatgggggca ggtcaaacga ctgtcgctac aatccgtaat
    25861 caagaactcc agttcacaca tattctccaa gaaggtggag attatgtaac taaagatatc
    25921 tccaaggttt tgaaaacctc tcgcaaatta gcggaaggct tgaaactgaa ttacggggaa
    25981 gcctatccgc ctcttgcaag caaagaaacc ttccaagtag aggttattgg agaagtggaa
    26041 gcagtcgaag tgacggaagc ctacttgtca gaaattattt ctgcacgaat caagcacatc
    26101 cttgaacaaa tcaagcaaga attagataga aggcgtctat tggacctccc tggtggtatt
    26161 gtcttaatcg gtgggaatgc cattttacca ggtatggttg agcttgctca ggaagtcttt
    26221 ggcgtccgtg tcaagcttta tgttccaaat caagttggta tccgtaatcc agcctttgcg
    26281 catgtgatta gtttatcaga atttgcgggt caattaacag aagttaatct tttggctcag
    26341 ggagcgataa aaggtgagaa tgacttaagt catcagccaa ttagttttgg tgggatgctg
    26401 caaaaaacag ctcagtttgt acaatcaacg cctgttcaac cagctcctgc tccagaagta
    26461 gagtcggtgg cgcctacaga accaatggcg gatttccaac aagcttcaca aaataaaccg
    26521 aaattagcag atcgtttccg tggcttgatc ggaagcatgt ttgacgaata aagaggaaaa
    26581 ataaattatg acattttcat ttgatacagc tgctgctcaa ggggcagtga ttaaagtaat
    26641 tggtgtcggt ggaggtggtg gcaatgccat caaccgtatg gtcgacgaag gtgttacagg
    26701 cgtagaattt atcgcagcaa acacagatgt acaagcattg agtagtacaa aagctgagac
    26761 tgttattcag ttgggaccta aattgactcg tggtttgggt gcaggaggtc aacctgaggt
    26821 tggtcgtaaa gccgctgaag aaagcgaaga aacactgacg gaagctatta gtggtgccga
    26881 tatggtcttc atcactgctg gtatgggagg aggctctgga actggagctg ctcctgttat
    26941 tgctcgtatc gccaaagatt taggtgcgct tacagttggt gttgtaacac gtccctttgg
    27001 ttttgaagga agtaagcgtg gacaatttgc tgtagaagga atcaatcaac ttcgtgagca
    27061 tgtagacact ctattgatta tctcaaacaa caatttgctt gaaattgttg ataagaaaac
    27121 accgcttttg gaggctctta gcgaagcgga taacgttctt cgtcaaggtg ttcaagggat
    27181 taccgatttg attaccaatc caggattgat taaccttgac tttgccgatg tgaaaacggt
    27241 aatggcaaac aaagggaatg ctcttatggg tattggtatc ggtagtggag aagaacgtgt
    27301 ggtagaagcg gcacgtaagg caatctattc accacttctt gaaacaacta ttgacggtgc
    27361 tgaggatgtt atcgtcaacg ttactggtgg tcttgactta accttgattg aggcagaaga
    27421 ggcttcacaa attgtgaacc aggcagcagg tcaaggagtg aacatctggc tcggtacttc
    27481 aattgatgaa agtatgcgtg atgaaattcg tgtaacagtt gttgcaacgg gtgttcgtca
    27541 agaccgcgta gaaaaggttg tggctccaca agctagatct gctactaact accgtgagac
    27601 agtgaaacca gctcattcac atggctttga tcgtcatttt gatatggcag aaacagttga
    27661 attgccaaaa caaaatccac gtcgtttgga accaactcag gcatctgctt ttggtgattg
    27721 ggatcttcgc cgtgaatcga ttgttcgtac aacagattca gtcgtttctc cagtcgagcg
    27781 ctttgaagcc ccaatttcac aagatgaaga tgaattggat acacctccat ttttcaaaaa
    27841 tcgttaagta aatgaatgta aaagaaaata cagaacttgt ttttcgagaa gttgcagagg
    27901 ctagtctgag tgctcatcga gagagtggtt cggtctctgt cattgcagtt accaagtatg
    27961 tagatgtacc gacagcggaa gccttgcttc cgctaggtgt tcatcatatc ggtgaaaatc
    28021 gtgtagataa gtttctggaa aaatatgaag ctttaaaaga tcgagatgtg acttggcatt
    28081 tgattggtac cttgcaaaga cgtaaggtga aagatgtcat tcaatacgtt gattatttcc
    28141 atgcattgga ctcagtaaag ctagcagggg aaattcaaaa aagaagtgac cgagtcatca
    28201 agtgtttcct tcaagtaaat atttctaaag aagaaagcaa acacggtttt tcgagagagg
    28261 aactgctgga aatcttgcca gagttagcca gactagataa gattgaatat gttggtttaa
    28321 tgacgatggc accttttgag gctagcagtg agcagttgaa agagattttc aaggcgaccc
    28381 aagatttaca aagagaaatt caagagaaac aaattccaaa tatgcctatg accgagttaa
    28441 gtatgggaat gagtcgtgat tataaagaag cgattcaatt cggttccact tttgttcgta
    28501 taggtacatc attttttaag taggagagaa ccatgtcttt aaaagataga ttcgatagat
    28561 ttatagatta ttttacggag gatgaggatt caagtctccc ttatgaaaaa agagatgagc
    28621 ctgtgtttac tccagtaaat tcttcacagg aaccggctct cccaatgaat caaccttcac
    28681 agtcggctgg tacaaaagag aacaatatca ccagacttca tgcaagacaa caggaattgg
    28741 caaatcagag tcagcgtgca acggataagg tcattataga tgttcgttat cctagaaaat
    28801 atgaggatgc aacagaaatt gttgatttat tggcaggaaa cgaaagtatc ttgattgatt
    28861 ttcagtatat gacagaggtg caggctcgtc gttgtttgga ctatttggat ggagcttgtc
    28921 atgttttagc tggaaatttg aaaaaggtag cttctaccat gtatttgttg acaccagtga
    28981 atgttattgt aaatgttgaa gatatccgtt taccagatga agatcaacag ggtgagttcg
    29041 gttttgatat gaagcgaaat agagtacgat aatgattttt ttaattcgta tgatttataa
    29101 tgcagtggat atttactccc tgattttggt agccttcgct gtcatgtctt ggtttccagg
    29161 tgcctacgaa tccagtttag gtcgttggat tgtagcgttg gtgaaaccag tgcttgctcc
    29221 cttgcaacgc ctgcctttac agatagcggg tcttgattta tctgtttggg tcgcgattgt
    29281 tttggttcga tttttaggag aaaacctagt gcgttttctg gcgatgatag gatgaataaa
    29341 gggatttatc agcatttctc catagaagat cgtccatttc ttgacaaggg aatggaatgg
    29401 ataaagaagg tagaagatag ctatgctcct tttttaactc cttttatcaa tcctcatcag
    29461 gagaagctat taaagatttt ggccaaaacc tatggtcttg cttgtagcag tagtggggaa
    29521 ttcgtctcga gtgagtatgt tcgagtttta ttatacccag attatttcca accagagttt
    29581 tcagattttg aaatatctct ccaggaaatt gtgtattcca ataaatttga acatttaacg
    29641 catgctaaga ttttagggac agtcatcaat caattaggga ttgaacggaa actttttgga
    29701 gatatcctag tagatgaaga acgggcgcag attatgatta atcagcagtt tcttcttctc
    29761 tttcaagatg gactaaagaa aattggtcgt atacctgttt cgctggagga acgtcctttc
    29821 accgagaaaa tagataagct agaacagtat cgagaactgg atttatctgt gtctagtttt
    29881 cgattagatg ttcttttatc aaatgttttg aaactatcta ggaatcaagc aaaccagttg
    29941 attgaaaaga aacttgtcca agtaaattat catgtggtag acaaatcaga ttacactgtt
    30001 caagttggag acttgattag tgtgagaaaa tttggtcgct tgagattact tcaagataag
    30061 ggacaaacga aaaaagagaa gaaaaaaata accgtccagt tattattaag taagtgagga
    30121 atagaatgcc aattacatca ttagaaataa aggacaagac ttttggaact cgattcagag
    30181 gttttgatcc agaagaagtc gatgaatttt tagatattgt ggttcgtgat tacgaagatc
    30241 ttgtgcgtgc gaatcatgat aaaaatttgc gtattaagag tttagaagag cgtttgtctt
    30301 actttgatga aataaaagat tcattgagcc agtctgtatt gattgctcag gatacagctg
    30361 agagagtgaa acaggcggcg catgaacgtt caaacaatat cattcatcaa gcagagcaag
    30421 atgcgcaacg cttgttggaa gaagctaaat ataaggcaaa cgagattctt cgtcaagcaa
    30481 ctgataatgc taagaaagtc gctgttgaaa cagaagaatt gaagaacaag agccgtgtct
    30541 tccaccaacg tctcaaatct acaattgaga gtcagttggc tattgttgaa tcttcagatt
    30601 gggaagatat tctccgtcca acagctactt atcttcaaac cagtgatgaa gcctttaaag
    30661 aagtggttag cgaagtactt ggagaaccga ttccagctcc aattgaagaa gaaccaattg
    30721 atatgacacg tcagttctct caagcagaaa tggaagaatt acaagctcgt attgaggtag
    30781 ccgataaaga attgtctgaa tttgaagctc agattaaaca ggaagtggaa actccaactc
    30841 ctgtagtgag tcctcaagtt gaagaagagc ctctgctcat ccagttggcc caatgtatga
    30901 agaaccagaa gtagctccag tgcatccgac aggtccaaca ccagctacag aaactgttga
    30961 ttcagcaccg ggatttgaag caccgcaaga atctgttaca attttataag aaatattctg
    31021 agaacaatat cttatcctta tattcccagc gagcaggaga tggtgtgagt cctgcattcc
    31081 ctatcgataa gattatcctc tcaaactatc aagtctgaat ctagtaagat ttgacgttcc
    31141 ccacgttacg ggataagagg gagaaagact aaaacttttt ccgaataaag gtggtaccac
    31201 gattttcgtc ctttttggaa gtcgtggttt ttaatttgtt attatttata aaggagatac
    31261 catgaaactc aaagacaccc ttaatcttgg gaaaactgaa ttcccaatgc gtgcaggcct
    31321 tcctaccaaa gagccagttt ggcaaaagga atgggaagat gcaaaacttt atcaacgtcg
    31381 tcaagaattg aaccaaggaa aacctcattt caccttgcat gatggccctc catacgctaa
    31441 cggaaatatc cacgttggac atgctatgaa caagatttca aaagatatca ttgttcgttc
    31501 taagtctatg tcaggatttt acgcaccatt tattcctggt tgggatactc atggtctgcc
    31561 aatcgagcaa gtcttgtcaa aacaaggtgt caaacgtaaa gaaatggact tggttgagta
    31621 cttgaaactt tgccgtgagt acgctctttc tcaagtagat aaacaacgtg aagattttaa
    31681 acgtttgggt gtttctggtg actgggaaaa tccatatgtg accttgactc ctgactatga
    31741 agcagctcaa attcgtgtat ttggtgagat ggctaataag ggttatatct accgtggtgc
    31801 taagccagtt tactggtcat ggtcatctga gtcagccctt gctgaagcag agattgaata
    31861 ccatgacttg gtttcaactt ccctttacta tgccaacaag gtaaaagatg gcaaaggagt
    31921 tctagataca gatacttata tcgttgtctg gacaacgact ccatttacta tcacagcctc
    31981 tcgtggtttg acggttggtg cagatattga ttacgttttg gttcaacctg ctggtgaagc
    32041 tcgtaagttt gtcgttgctg ctgaattatt gactagcttg tctgagaaat ttggctgggc
    32101 tgatgttcaa gttttggaaa cttaccgtgg ccaagaactc aaccacatcg taacagaaca
    32161 cccatgggat acagctgtag aagagttggt aattcttggt gaccacgtta cgactgactc
    32221 tggtacaggt attgtccata cagcccctgg ttttggtgag gacgattaca atgttggtat
    32281 tgctaataat cttgaagtcg cagtgactgt tgatgaacgt ggtatcatga tgaagaatgc
    32341 tggtcctgaa tttgaaggtc aattctatga aaaggtagtt ccaactgtta ttgaaaaact
    32401 tggtaacctc cttcttgccc aagaagaaat ctctcactca tatccatttg actggcgtac
    32461 taagaaacca atcatctggc gtgcagttcc acaatggttt gcctcagttt ctaaattccg
    32521 tcaagaaatc ttggacgaaa ttgaaaaagt gaaattccac tcagaatggg gtaaagtccg
    32581 tctttacaat atgatccgtg accgtggtga ctgggttatc tctcgtcaac gtgcttgggg
    32641 tgttccactt cctatcttct acgctgaaga tggtacagct atcatggtag ctgaaactat
    32701 tgaacacgta gctcaacttt ttgaagaaca tggttcaagc atttggtggg aacgtgatgc
    32761 caaagacctc ttgccagaag gatttactca tccaggttca ccaaacggcg agttcaaaaa
    32821 agaaactgat atcatggacg tttggtttga ctcaggttca tcatggaatg gagtggtggt
    32881 aaaccgtcct gaattgactt acccagccga cctttaccta gaaggttctg accaataccg
    32941 tggttggttt aactcatcac ttatcacatc tgttgccaac catggcgtag caccttacaa
    33001 acaaatcttg tcacaaggtt ttgcccttga tggtaaaggt gagaagatgt ctaaatctct
    33061 tggaaatacc attgctccaa gcgatgttga aaaacaattc ggtgctgaaa tcttgcgtct
    33121 ctgggtaaca agtgttgact caagcaatga cgtgcgtatc tctatggata tcttgagcca
    33181 agtttctgaa acttaccgta agattcgtaa cactcttcgt ttcttgattg ccaatacatc
    33241 tgactttaac ccagctcaag atacagtcgc ttacgatgag cttcgttcag ttgataagta
    33301 catgacgatt cgctttaacc agcttgtcaa gaccattcgt gatgcttacg caaactttga
    33361 attcttgaca atctacaagg ccttggtgaa ctttatcaac gttgacttgt cagccttcta
    33421 ccttgatttt gccaaagatg ttgtttacat tgaaggtgcc aaatcactgg aacgccgtca
    33481 aatgcagact gtcttctatg acattcttgt caaaatcacc aaactcttga ctccaatcct
    33541 tcctcacact gcggaagaaa tctggtcata tcttgagttt gaagccgaag acttcgtcca
    33601 attgtcagaa ttaccagaag ttcaaacttt tgctaaccaa gaagaaatct tggatacatg
    33661 ggcagccttc atggacttcc gtggacaagc acaaaaagcc ttggaagaag ctcgtaatgc
    33721 aaaagttatc ggtaaatcac ttgaagcaca cttgacagtt tatccaaatg aagttgtgaa
    33781 aactctactc gaagcagtaa acagcaatgt agctcaactt ttgatcgtgt ctgagttgac
    33841 catcgcagaa gaaccagctc cggaagctgc ccttagcttc gaagatgtag tcttcacagt
    33901 tgaacgcgct gcaggtgaag tatgtgaccg ttgccgtcgt attgacccaa caacagcaga
    33961 acgtagctac cacgcagtca tctgtgacca ctgtgcaagt atcgtagaag aaaactttgc
    34021 agatgcagtc gcagaaggat ttgaagagaa ataagattga aaagtctagg taaaactcaa
    34081 tttgagaaga aaagacaact aattttatag tctattaaac gcattgtatc acgtttttga
    34141 acacctgata tgatgcgttt tttatttatt ttaaaaattt gcgaggtatg actttttata
    34201 ctcaacaaga atcaaagaga aacttagcaa gctaacagta gtaatataaa ataggaattt
    34261 gatattaggg ataagattgg taaatagtgt aatattttta caacaataaa tttatatagt
    34321 tatttctggt ttctgaaaag tattatatta tattttattt catattatac aaatttttat
    34381 tttataatat cagaaaatac tttttttaaa aagcaaatat gatacaattt tatttgaaaa
    34441 aaaataaaaa aggagatttt attatgaaat caagtggtat cgtcggtctt gtgggaggaa
    34501 ttttacttct tatttgtcct tttgtcttgt tgggaacagc ggtaaataca gctgctacaa
    34561 ctcttaatgg aggagctact gcaggggctt tttcaggtgt tgccttactc ttgaatgcct
    34621 tgaagattgc aaatcttgtt cttggtatca ttgctattgt ttactataaa ggagataagc
    34681 atgtaggtgc agctccgtct gtactaatga ttgtttctgg tgaagttagt ctcattccat
    34741 tcttaggatg ggttgggggg attcttgcta ttatcggagg atctctattc cttgcaacat
    34801 tgaagaaatt caaatcagaa gaataaaagg tattttagca tgaaaagaac aaaaaagttt
    34861 atcggtatag gagtagctct attatctctt tctcttctag ttgcatgtgg aacataaagt
    34921 tcaaagaata cttcaacaag taatgatgag aagacagtag caacatccaa tagttcaaaa
    34981 gaaacaatca ctttcgatac accggttgta acagacgatg cgattgaatc aatacgcact
    35041 tatgcagatt atatagatct ttataaaaat atttttgatg attattttac taaagctgag
    35101 gaaggtttca aaggcacaac tatggaaaat aatgactcgt ttactaaact aaaagagtca
    35161 actcaaaaat tattcgatgc gcagaaaaaa aggttaaata atgaagatag aatagaaaca
    35221 accaaaaaca atgtgattgc caaacattgt caaacagtcc tttccttttt ggttttgact
    35281 agcttttttg tgaaaaattg tgtaaaatag aatagataaa cgaggggaaa cctcggaaaa
    35341 tttaaaggag aatccatcta atggtaaaat tggtttttgc tcgccacggt gagtctgaat
    35401 ggaacaaagc taaccttttc actggttggg ctgatgttga tttgtctgaa aaaggtacac
    35461 aacaagcgat tgacgctggt aaattgatca aagaagctgg tatcgaattt gaccaagctt
    35521 acacttcagt attgaaacgt gctatcaaaa caactaactt ggctcttgaa gcttctgacc
    35581 aattgtgggt tccagttgaa aaatcatggc gcttgaacga acgtcactac ggtggtttga
    35641 ctggtaaaaa caaagctgaa gctgctgaac aatttggtga tgagcaagtt cacatctggc
    35701 gtcgttcata cgatgtattg cctccaaata tggaccgtga tgatgagcac tcagctcaca
    35761 cagaccgtcg ttatgcttca cttgacgact cagtcattcc agatgctgaa aacttgaaag
    35821 tgactttgga acgtgctctt ccattctggg aagataaaat cgctccagct cttaaagatg
    35881 gtaaaaacgt attcgtagga gctcacggta actcaatccg tgcccttgta aaacacatca
    35941 aaggtttgtc agatgatgag atcatggacg tggaaatccc taacttccca ccattggtat
    36001 tcgaattcga cgaaaaattg aacgttgttt ctgaatacta ccttggaaaa taaaaaattg
    36061 taagcctagg attgatttct aggcttttta tgttagtatg gaagtatgat aaggaataaa
    36121 aaacaagatt atgtactggc ctacaagcaa ccagcttcaa ccacttacat gggttgggaa
    36181 gaagaagctt taccgatagg caatggttct ttaggagcaa aagtatttgg ccttataggg
    36241 gctgaacgga ttcaatttaa tgaaaaaagt ctctggtctg gaggtccact tcctgatagt
    36301 tcagattatc agggtggaaa tcttcaggat cagtatgttt ttttagctga gattcggcag
    36361 gctttggaga agagagatta caatctggct aaggaactgg ctgagcagca cctaattggg
    36421 ccaaaaacga gtcaatatgg gacctatctg tcttttgggg atattcacat tgagttcagc
    36481 cagcaaggta cgactttgtc tcaggtgacg gactatcaga gacagctgaa tattagtaag
    36541 gcacttgcga cgacttctta tgtctataag ggaacgcgat ttgaacgtaa agcttttgcg
    36601 agttttccag atgatctctt gattcaatgt tttactaagg aagggttgga aactctagat
    36661 tttactatag aactatcctt gacctgtgat ttggcttctg atggaaagta tgagcaggaa
    36721 aaatctgatt acaaggagtg taagttggat attactgatt ctcatatctt gatgaaggga
    36781 agagttaagg ataatgatct gcggtttgct agttatctag cttgggaaac ggatggagat
    36841 attagagttt ggtcagatag ggttcagata tcaggagcca gttatgccaa tctcttcttg
    36901 gccgctaaga cggattttgc ccaaaatcct gctagcaatt atcgcaagaa actagattta
    36961 gagcaacagg tgatagactt ggtggacaca gctaaagaaa agggctatac ccaattgaaa
    37021 tcaaggcata tcgaggacta ccaagcctta ttccagcgtg ttcaattgga tttggaagct
    37081 gatgttgacg catccactac agatgatttg ttaaaaaatt ataagccaca agaagggcag
    37141 gctttggagg agctgttctt ccagtatgga cggtatttat tgattagttc gtccagagac
    37201 tgcccagatg ctctaccagc taacctacag ggagtctgga atgcggtcga caatcctcct
    37261 tggaattcgg actatcactt aaatgtcaat ctgcagatga attattggcc agcctatgtt
    37321 accaatctcc tagagacggt ctttccagtc atcaactatg tagatgattt gcgtgtctat
    37381 ggtcgtctag cggctgtaaa gtatgcagga atcgtctctc aaaaaggtga ggaaaatggt
    37441 tggttggttc atactcaagc gactcccttt ggttggacgg cacctggttg ggattactat
    37501 tggggttggt caccagctgc caatgcgtgg atgatgcaaa ccgtttatga agcctattca
    37561 ttttataggg accaagacta tctcagggag aaaatttatc ccatgttgag ggaaacggtt
    37621 cgtttttgga atgccttttt acataaggat cagcaggcgc agcgttgggt ctcttctccg
    37681 tcttattccc cagaacatgg gccgatttcg attggcaata cctatgacca atctctgatt
    37741 tggcagttat ttcatgattt tattcaggct gctcaggaat tgggactgga tgaggacttg
    37801 ttgactgagg ttaaggagaa gtctgattta ctaaatcctt tgcaaatcac tcaatctggt
    37861 cgaatcaggg agtggtatga ggaggaagag cagtattttc aaaatgagaa agtggaggcc
    37921 cagcatcggc acgcttccca tctagtggga ctctattctg gcaatctctt tagctacaag
    37981 ggacaagagt atattgaagc ggcgcgtgct agcctcaatg atcgtggaga tggcggcaca
    38041 ggctggtcca aggctaataa gatcaatctc tgggcgcgtt tgggagatgg caatcgagcc
    38101 cataaattat tggcagagca gttaaagaca tccaccttgc caaatctttg gtgtagccat
    38161 cctccttttc agatagatgg taattttggt gctactagtg gcatggcaga aatgttactc
    38221 cagtctcatg cagcttatct ggtacctcta gctgccctac ctgatgcttg gtcaacaggt
    38281 tctgtttcag gcttaatggc acgtggacat tttgaagtga gcatgagctg ggaagataaa
    38341 aaactcttac agttgaccat tttatcaagg agtggaggag atttgcgagt ttcttatcca
    38401 gatattgaga agagtgtgat taaaatgaat caagaaaaaa taaaagcgaa atgcatgggg
    38461 aaagattgta tttcggtggc aacagcagaa ggtgatcttg ttcaatttta tttttaagaa
    38521 gatgttataa ggcagtaatt tgaaacttcc ttttaataag gatttgagaa tataggcagt
    38581 ttccaactag ttgaaaaagc gttataatga taataggaag taatactcaa tgaaaatcaa
    38641 agagcacaaa ctaggaagct agccgcaggt tgctcaaaac agtgttttga ggttgtagat
    38701 ggaagctgac gtggtttgaa gagagatttt cgaggagtat aatttgtttg atagagggtg
    38761 ggtctgatgg cttatattga gatgaaacac tgttacaagc gttatcaggt tggggacacg
    38821 gagattgtgg ccaatcgtga tgtgaatttt gagattgaaa agggggagct ggttattatc
    38881 cttggtgctt caggtgcagg caagtcaaca gttcttaacc ttcttggggg aatggatacc
    38941 aatgatgaag gggaaatctg gattgatggt gttaatattg cggattatag ttcccaccag
    39001 cgtaccaatt accgtagaaa tgatgtgggg tttgtttttc agttttataa tctagtttct
    39061 aatctgacag ctaaggaaaa tgtggaactg gcttctgaaa ttgtgacaga tgccttgaat
    39121 cctgatcagg tcttgacaga tgtaggtctg gctcatcgtc tcaataactt tccagcccag
    39181 ctttctggag gggagcaaca gcgagtctcc attgcacgcg cggtagccaa aaatcctaaa
    39241 attctccttt gtgatgaacc gactggagcc ttggattatc agacgggcaa gcaggttttg
    39301 aaaattctcc aagacatgtc tcgtcaaaag ggagcgacgg tgatcatcgt gactcataat
    39361 ggagctttgg cgcccattgc tgatcgcgtg attcaaatgc acgatgccag tgtcaaggat
    39421 gtggtgctca accagcatcc tcaggatatt gacagtttgg agtactagca tgatcaagcg
    39481 aaaaacttat tggaaggact taattcagtc cttcacaggc tccaaggggc gttttttatc
    39541 catcttgatc ctgatgatgt tgggatctct agccttagta ggcctcaaag taaccagtcc
    39601 caacatggag gcgacagcta atgcttattt aacaactgct caaaccttgg atttggcagt
    39661 catgtctaac tatggcttgg atcaagcaga ccaagaagaa ctaaaacaga cggagggcgc
    39721 agaggtcgag tttggctatt tgacagatgt gactatggat aatgggcagg atgccattcg
    39781 gctgtactcc aaaccagagc gaatttcaac ctttcagcta agaaagggac gacttcctca
    39841 gtcagacaag gaaatcgctt tggccactca tttgcaaggc caatacagcg tgggacagga
    39901 gattagtttt aaagaaaaag aagagggtca ttcctcttta aaagaccata cttataccat
    39961 tactggtttt gtggattcgg ctgaaatcct ctcccagcga gatatgggct acgcaggaag
    40021 tggaagtggg actctgacag cctatggggt gattttacct agtcagtttg atcagaaagt
    40081 ctacaatata gctcgtttga aatatcaaga tttagcaggt ttaaatgcct tttcatcagc
    40141 ttatgaagaa aaatccaagc aacatcagga agaccttgaa caaactttat cagataatgg
    40201 caaggtacgt ctgcaacttt tgaaaaaaga aggacaagag tctctagaca aggggcaaga
    40261 gacccttgac aaggctcaga ctaatttgca ggaaggcaag cgtcgtttag cagctgctca
    40321 agctcgtata caggctcaag aaagtcaact agccttgttt cctcaagttc agagagagca
    40381 ggctagtgct caacttaccc aagccaagca ggaattgggc aaggaagagg ataaactaaa
    40441 gcaagctgaa caaaatctag cccaagaaaa ggaaaagtta gaaaaacatc agcaagtctt
    40501 ggacgatttg gcggagccaa ggtatcaggt ctataatcgt cagaccatgc caggtggtca
    40561 gggctatctt atgtatagca atgcttcatc cagtattcga gcagtgggca atatctttcc
    40621 tgtggtactt tatgccgtag catccatggt gacctttacg accatgactc gctttgtaga
    40681 cgaagagcga actcatgcag ggatttttaa ggccttgggt taccgtagta aggatattat
    40741 caccaagttt ctcctttatg gactagtagc tgggactgtc ggaacggctc taggtagtat
    40801 acttggtcat tatttgctag ccagtgtaat ttcaagtgtc attacaaaag gcatggtggt
    40861 gggagaaacc cagattcagt tctattggac ctatagctta ctagctcttg tcttgagctg
    40921 gttggcgagt gtgttaccag cctatctggt ggcttggagg gaacttcatg ccgaagcagc
    40981 ccagcttcta cttcctaaac ctcctgtcaa aggagctaaa atcttattgg agcgtatcgg
    41041 ttttatctgg cgtcgtctca gttttactca taaggtaaca gcccgcaaca tctttcgtta
    41101 taagcagaga atgttgatga caatctttgg tgtggcaggt tctgtagctc tgctctttgc
    41161 aggtttggga atccaatctt ctgtagcagg agttccgtct aaacagtttc aacaaatcca
    41221 acagtatcag atgcttgtct ctgaaaatcc tagtgcgacc aatcaggaca aggtagagct
    41281 agcagaagtg ttgaaagggc aggagatact agcctaccag aaaatctatt ctaaaacgct
    41341 agacaaggat ttcaaaggca aggctggtct tcaaaacatt actcttatga tgatagagaa
    41401 ggaagatttg actcccttta tccatcttca acatcatcag caggagctga cattaaaaga
    41461 tggcatcgtt attacagcta aactcgccca gctggcaggt gtcaaggttg ggcagacttt
    41521 agaaattgaa ggtaaggaac taaaggtcgc tgctattact gagaactacg ttggtcactt
    41581 tatttatatg agtcaggcta gctatgagca actttacgga cagctacccc aagccaacac
    41641 ttatctggtc tcattaaggg ataccagtgc aactagtatc gaaagtcagg cgggcttgct
    41701 tatgaatcaa tctgcggtgt ccagcgttgt ccaaaatgct tcagccattc gactctatcg
    41761 ctagctcact caatcagacc atgaccatct tggtcatcgt atcagttcta ttagctattg
    41821 tcatccttta caatctgacc aatatcaacg tagctgagag aatccgtgaa ctctccacta
    41881 tcaaggttct cggttttcat aataatgaag tcaccctcta catttaccgt gagacgattg
    41941 tgctgtccct tgtgggaatc gtacttggtc tggtagctgg tttctattta caccaatttt
    42001 tgattcaaat gatttcgcct gcgactattc tcttttatcc gcaggtaggc tgggaagtct
    42061 atgtaatccc agtggcaaca gtaagcatca ttttgacctt gcttggtttc ttcgtcaatt
    42121 attatctgag aaaggttgat atgttagaag ccctgaaatc tgtagagtaa ggtagttatt
    42181 tttagctgat tgaacttcta tttactaata ttcaaaaatc ctctgtcaca aagagcaggg
    42241 agctctttgt gacagaggat tttttctata gggctttagc agctgcaatt gcggcttcga
    42301 agtttggctc agaattgata ttatccacgt attcaacgta gcgaatcgta ttgtcagtat
    42361 cgaggacaaa gactgcgcgt gctaataggt gccattcgtt gatcaagagg gcataatcgc
    42421 gcccgaaaga atggtcaaag tagtctgaaa gcataatggc attgtcaagg ccttcagcac
    42481 cgcaccaacg tttttgagca aaaggtaggt ccattgaaac agtcaatacg accgtgttgt
    42541 ccagtccagc caattcttca ttaaaacgac gtgtttgagt tgagcagatg cctgtatcga
    42601 tagaaggaac gacactcaag acttttttct tgccatcaaa atcagccaga gattttttag
    42661 aaagatctgt tgtagtaaga gaaaaatcaa gcgccttgtc gccgacttgt agttgtttac
    42721 ctgtaaagct cacaggattt ccgagaaaag ttaccatagg atactccaat cttttttctt
    42781 ccattgtatc tgaaacagtc agaattttcc aatgatttga ccggaaatgt gggcatagaa
    42841 aaaacgccag ctcacatgag aatgacgttt ttcagaggct tattttgcca atccttcagc
    42901 aatcttgtca aggttgtatt tcatcatgct gtagtagctg tcgccttctt taccttgttc
    42961 tgcgatagag tcagtaaaga tttgtgcgta gattgggatg tttgtgtctt gagaaacagt
    43021 tttcattgga cggtcatcca cacttgattc tacaaagagt gatggaactt ttgtttggcg
    43081 aagtttttca accaaggtct tgatttgttc aggagttcct tcttcttcag tattgatttc
    43141 ccagatgtaa gcacttggga caccataggc tttagagaag tatttgaatg ctccttcgct
    43201 ggttacaatg agtttctttt cagcagggat cttattaaat ttatccttac tttctttatc
    43261 aagtttgtct aacttatcag tatattcttt gagatttttt tcatagaatt ctttattgtt
    43321 agggtctttg gcgctcaatt gtttggcgat atttttagca aaaataatac cgttttcaag
    43381 gttaagccaa gcgtgtgggt cttcttttcc tttttcattt tgaccttcaa ggtagataac
    43441 atcaacgccg tcgctgactg cgaagtagtc tttgttttca gttttcttgg cattttctac
    43501 caattttgta aaccaagcat tgccacctgt ttcaaggttg ataccgttat agaaaatcaa
    43561 atcagcctca gaagttttct taacgtcttc aggaagtggt tcgtattcgt gtgggtcttg
    43621 cccaatcgga acgatactat gaaggtcaat tttgtcacca gcaatatttt tagtaatatc
    43681 agcgatgatt gagtttgtag caacaacttt tagtttttga ccagaagttg tatctttttt
    43741 tccgctagca catgctacaa gaatgattgc agaaagaaag agaacgagta atgtacctaa
    43801 ttttttcatt agatcctcca aattattagg gctttgcccc ttattttaac aaatgtttat
    43861 ttttcagttt caaatatcgt tgtttgggag cgataaagaa gctaatgaga aagaaactag
    43921 cagctgtaag cacgatacta gaacctgccg caacattaaa actatagcca ataaagagtc
    43981 ccaaaactga agcagtagct ccgaaggttg aggaaaggaa aatcatgctt ttcagactat
    44041 tagcatacag ataagcagtt gcagctgggg taatcagcat ggctacaatc aggatagttc
    44101 cgacactttg catggctgtc acagacacga gagtcaggag taccatgaga aggtagtgat
    44161 agaaattgac aggcattccc atggctttag ccaagagttc atcaaaggaa gttatcaaga
    44221 gttgcttgaa gaaaatccag attaacaaga ggatggctgc ccccacaccc atagtaataa
    44281 acatatccgt atcttggacg gccaggatat taccaaaaag gatatggaaa aggtcagttg
    44341 aacttttagc gacaccaatc aagatgatac cgagggctaa gaaagaagaa aaggtaatgc
    44401 cgatggcggt atcgcttttg ataatcgagt ttcctttgat gtaggtaatg atgatggcag
    44461 ctagcaatcc aaagacaatg gctccgataa agaagtcaag gcccaagatg aaggagaggg
    44521 ctacacctgg taagacagca tgtgaaatgg catctcccat gagtgacatc ccgcgtagaa
    44581 taatgaaaca tcccacagct ccagctacaa tcccgacgac aatagctgtt atcaaggcat
    44641 tttgtaggaa atggaatttt tgcaatccat cgataaattc tgcaatcata ggtcacctcc
    44701 attgaaaaag agttgattac cgtaagcttc ttttagattg gtttcggtaa aagtttcttt
    44761 tgttggacca aaggcaatca cttctcgatt gacaagtaag acttgatcga agtagtgggg
    44821 aatcttgctg aggtcgtggt gaacgatgag aaccgtcttc ccagcttttt tcaaatctct
    44881 cagcgtattc atgatgattt cctcactgac agagtcaatc ccagcaaagg gttcatccaa
    44941 gaggatatag tcggcttcct gcaccaaaca tctggcaatc aaaacccgct ggaattgacc
    45001 tccagacagt tgactaattt gacgttcagc gtagtcagct aggccgacga tttcaagggc
    45061 ctcttgcact ttcttccaat gtttagcctt taaacttcga aagagaggaa tagagggaaa
    45121 tagtcctaac gagacgcatt ccttgacctt gatgggaaag ttgtagtcga tattgatttt
    45181 ttgttcgaca taggcaattc ggtgtaagga ttttttaact tccttgtcat cgagaaatgc
    45241 ctgaccttga tgtgggataa ttcccagcat accttttaat agtgttgatt tcccagcgcc
    45301 gtttggacca atgatgccgg taattgttgg tccatggagc actagtgaaa tatccttaag
    45361 tgccaacgtt tctttgtagg agacactgag gttttcgata cgtatcataa acttgtattc
    45421 ctcctgtctc ttaatataca ttaaaaaaaa aattaagtca agttaatttt tgaaaaaatt
    45481 aaaataataa ctgaaaaata ga
//
LOCUS       DAVBCR010000009        41007 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCR010000009 DAVBCR010000000
VERSION     DAVBCR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.009"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            83..1057
                     /gene="folP"
                     /locus_tag="VQ508_000431"
     CDS             83..1057
                     /gene="folP"
                     /locus_tag="VQ508_000431"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2489486.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            1059..2381
                     /locus_tag="VQ508_000432"
     CDS             1059..2381
                     /locus_tag="VQ508_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2489487.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQDTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCRPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            2362..2916
                     /gene="folE"
                     /locus_tag="VQ508_000433"
     CDS             2362..2916
                     /gene="folE"
                     /locus_tag="VQ508_000433"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2489488.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            2959..3771
                     /gene="folK"
                     /locus_tag="VQ508_000434"
     CDS             2959..3771
                     /gene="folK"
                     /locus_tag="VQ508_000434"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2489489.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(3921..4238)
                     /locus_tag="VQ508_000435"
     CDS             complement(3921..4238)
                     /locus_tag="VQ508_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2489490.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            4594..5040
                     /gene="rplM"
                     /locus_tag="VQ508_000436"
     CDS             4594..5040
                     /gene="rplM"
                     /locus_tag="VQ508_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2489491.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            5059..5451
                     /gene="rpsI"
                     /locus_tag="VQ508_000437"
     CDS             5059..5451
                     /gene="rpsI"
                     /locus_tag="VQ508_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2489492.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            5800..5952
                     /locus_tag="VQ508_000438"
                     /pseudo
     CDS             5800..5952
                     /locus_tag="VQ508_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6108..6446
                     /locus_tag="VQ508_000439"
     CDS             6108..6446
                     /locus_tag="VQ508_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2489493.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            6636..8072
                     /locus_tag="VQ508_000440"
     CDS             6636..8072
                     /locus_tag="VQ508_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2489494.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVI
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGENREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            8124..8363
                     /locus_tag="VQ508_000441"
     CDS             8124..8363
                     /locus_tag="VQ508_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2489495.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            8493..8807
                     /locus_tag="VQ508_000442"
     CDS             8493..8807
                     /locus_tag="VQ508_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2489496.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            8925..10904
                     /locus_tag="VQ508_000443"
     CDS             8925..10904
                     /locus_tag="VQ508_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2489497.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            10914..11228
                     /locus_tag="VQ508_000444"
     CDS             10914..11228
                     /locus_tag="VQ508_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2489498.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            11259..11765
                     /locus_tag="VQ508_000445"
     CDS             11259..11765
                     /locus_tag="VQ508_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489499.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            11845..13191
                     /gene="celB"
                     /locus_tag="VQ508_000446"
     CDS             11845..13191
                     /gene="celB"
                     /locus_tag="VQ508_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2489500.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            13571..13747
                     /locus_tag="VQ508_000447"
     CDS             13571..13747
                     /locus_tag="VQ508_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489501.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            14149..16362
                     /locus_tag="VQ508_000448"
     CDS             14149..16362
                     /locus_tag="VQ508_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2489502.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(16584..17060)
                     /locus_tag="VQ508_000449"
     CDS             complement(16584..17060)
                     /locus_tag="VQ508_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2489503.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            17283..20486
                     /locus_tag="VQ508_000450"
     CDS             17283..20486
                     /locus_tag="VQ508_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2489504.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            20866..21251
                     /gene="tnpA"
                     /locus_tag="VQ508_000451"
                     /pseudo
     CDS             20866..21251
                     /gene="tnpA"
                     /locus_tag="VQ508_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21630..22259)
                     /locus_tag="VQ508_000452"
     CDS             complement(21630..22259)
                     /locus_tag="VQ508_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2489505.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(22269..23270)
                     /locus_tag="VQ508_000453"
     CDS             complement(22269..23270)
                     /locus_tag="VQ508_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2489506.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(23301..23942)
                     /locus_tag="VQ508_000454"
     CDS             complement(23301..23942)
                     /locus_tag="VQ508_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2489507.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(23961..24776)
                     /locus_tag="VQ508_000455"
     CDS             complement(23961..24776)
                     /locus_tag="VQ508_000455"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2489508.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            25047..25481
                     /locus_tag="VQ508_000456"
     CDS             25047..25481
                     /locus_tag="VQ508_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2489509.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            25493..26683
                     /locus_tag="VQ508_000457"
     CDS             25493..26683
                     /locus_tag="VQ508_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2489510.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            26694..27185
                     /locus_tag="VQ508_000458"
     CDS             26694..27185
                     /locus_tag="VQ508_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2489511.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            27200..27979
                     /locus_tag="VQ508_000459"
     CDS             27200..27979
                     /locus_tag="VQ508_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2489512.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            27966..28784
                     /locus_tag="VQ508_000460"
     CDS             27966..28784
                     /locus_tag="VQ508_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2489513.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            28784..29077
                     /locus_tag="VQ508_000461"
     CDS             28784..29077
                     /locus_tag="VQ508_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2489514.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            29099..31000
                     /locus_tag="VQ508_000462"
     CDS             29099..31000
                     /locus_tag="VQ508_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2489515.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            31060..32061
                     /locus_tag="VQ508_000463"
     CDS             31060..32061
                     /locus_tag="VQ508_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2489516.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(32171..32506)
                     /locus_tag="VQ508_000464"
     CDS             complement(32171..32506)
                     /locus_tag="VQ508_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489517.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(32517..32759)
                     /locus_tag="VQ508_000465"
     CDS             complement(32517..32759)
                     /locus_tag="VQ508_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489518.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(32770..33312)
                     /locus_tag="VQ508_000466"
     CDS             complement(32770..33312)
                     /locus_tag="VQ508_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2489519.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSVLGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(33328..33522)
                     /locus_tag="VQ508_000467"
     CDS             complement(33328..33522)
                     /locus_tag="VQ508_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489520.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            33688..34638
                     /gene="rsmH"
                     /locus_tag="VQ508_000468"
     CDS             33688..34638
                     /gene="rsmH"
                     /locus_tag="VQ508_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2489521.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIHQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            34650..34967
                     /gene="ftsL"
                     /locus_tag="VQ508_000469"
     CDS             34650..34967
                     /gene="ftsL"
                     /locus_tag="VQ508_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2489522.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            34971..37223
                     /gene="pbpX"
                     /locus_tag="VQ508_000470"
     CDS             34971..37223
                     /gene="pbpX"
                     /locus_tag="VQ508_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2489523.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            37225..38205
                     /locus_tag="VQ508_000471"
     CDS             37225..38205
                     /locus_tag="VQ508_000471"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2489524.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            38814..40919
                     /locus_tag="VQ508_000472"
     CDS             38814..40919
                     /locus_tag="VQ508_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2489525.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
BASE COUNT        12831 a         6956 c         8959 g        12261 t
ORIGIN      
        1 aggaaccgac ctctttctac tagagaaaga tgaatgtaat cgtgtccatc tttttctttt
       61 tatggtaaaa tagaaaaata atatgatgaa aatccttgag ggagtgaccg atatgtcaag
      121 taaagccaat catgcaaaga cagttatttg cggaattatc aatgtaaccc cagactcctt
      181 ttcggacggt ggtcaatttt ttgctcttga gcaggcactc cagcaggctc gtaaattgat
      241 agcagaagga gccagtatgc tcgatatcgg cggagaatcg actcggccgg gcagtagcta
      301 tgttgagata gaagaggaaa tccagcgtgt tgttccagtg atcaaagcga ttcgcaagga
      361 aagtgatgtc cttatttcta tcgatacttg gaaaagtcag gtggcagagg ctgctttggc
      421 tgcgggtgcc aatctagtta atgatatcac tggtctcatg ggtgatgaaa aaatggccca
      481 tgtggttgct aaagctggag cgaaagtggt catcatgttt aatccagtta tggcgcgacc
      541 tcagcatcct agttcgctta tcttccctca ttttggcttt ggtcaaacct ttacagaaaa
      601 agagttagct gactttgaaa cattgccaat tgaagaattg atggaggctt tctttgaacg
      661 agcactagcg agagcggcag aagctggtat tgcaccagaa aatatcctgt tggatccagg
      721 aattggcttt ggtctgacca agaaagaaaa tctgcttctt ttacgggacc tggataaact
      781 acatcagaag ggctatccaa tctttctcgg agtgtcgcgc aagcgatttg tcatcaatat
      841 tctagaggag aatggttttg aagtcaatcc tgagacagag cttggtttcc gaaatcggga
      901 cacggcttcg gctcatgtaa ctagtatcgc tgcgagacag ggtgtagaag tggtgcgcgt
      961 gcatgacgta gctagtcaca ggatggcagt tgaaattgcc tctgccattc gtctggctga
     1021 tgaagcggaa aatttagatt taaaacagta taaataagat gaaagaaatt gaaaacaatc
     1081 agtggattgc taactaccgg acggatcaac cgcattttgg cttggaacga atggtggaac
     1141 tgttagcttt gcgtggcaat ccccatctca aactcaaggt tctccatatt ggagggacta
     1201 acggcaaggg ctcgactatt gcttttttga aaaagatgct agaaaagcta gggctgagag
     1261 ttggcgtgtt tagttcgccc tatctcattc attacacaga ccagattagc atcaatgggg
     1321 aatcgatctc agaagcgagg ctagaagctc tcatggcaga ctatcagtct ttgctggagg
     1381 gagaagcggt cgccaattta caggacacaa ccgagtttga gattatcaca gccctggcct
     1441 atgactactt tgcctcagag caagtagatg tggccatcat ggaagttggc atgggcggac
     1501 ttttggatag taccaatgtc tgtcggccca ttttgacagg aattacaact attggcttgg
     1561 atcatgtggc cctacttggt gacaccttgg aggccatagc agagcagaag gcaggtatta
     1621 tcaaacaagg gatgcccttg gtaacagggc gtattgctcc agaagccttg actgtgattg
     1681 accgcattgc ggaagggaaa gatgcgccga gacttgccta cgggacagat tatcaggttc
     1741 gtcatcaaga aagtgtggtg acaggggaag tctttgacta tacaagtgct gtcagacaag
     1801 gtcgcttcca gactagcctg cttggtttgt accaaataga gaatgctggg atggccatag
     1861 ctttacttga tactttttgt caagaagatg gtcgagagct agcaagcaat gactttcttg
     1921 gtcaagcctt ggaagaaaca agttggccag ggcgtttgga aatcgtgtca agagatccct
     1981 tgatgatttt ggatggagcc cataatcccc atgctatcaa ggccttgttg gtaaccttgc
     2041 aagaacgttt tgcggattat cataaggaaa tcctcttcac ttgtatcaaa accaaagcct
     2101 tggaggatat gttggacttg ctgggagcca tgccagatac cgagcttact ctaacacatt
     2161 ttgcggatag tcgggcgacg gatgaaagcg tgctgaaaga ggcagctaag tctagaaatc
     2221 tcagctacca agattggcat gattttctag agcagaattt gacagataaa aaagaagaga
     2281 aacaaacagt taggattgtc acaggttcct tgtatttctt gagccaagtg agggcctatc
     2341 tgatggagag gaagaacgag aatggataca caaaagattg aagcagctgt aaaaatgatt
     2401 atcgaggctg taggagagga cgctaatcgc gagggcttgc aggaaacacc tgctcgtgta
     2461 gcccgtatgt atcaagagat tttttcaggt cttggtcaaa cagcagagga acatttgtca
     2521 aaatcctttg aaattattga cgataatatg gtggtagaaa aggatatctt tttccatacc
     2581 atgtgtgaac accacttctt gccattttat ggtagagcgc acattgccta cattccagat
     2641 ggtcgtgtgg caggcttgtc taagctagcc cgtacggttg aagtttattc gaaaaaacca
     2701 caaattcaag aacgtttgaa tatcgaagtg gccgatgcct tgatggacta tctaggtgct
     2761 aaaggagcct ttgttgtcat tgaggcggaa catatgtgta tgagtatgcg tggtgttaga
     2821 aaaccaggca ctgcaacctt gacgacagta gctcgtggtc tatttgaaac agataaggac
     2881 ctccgcgacc aagcttatcg tttaatgggg ctataaaaag aatccgcttc aagcggattt
     2941 ttctagaaag gaatcattat ggatcaactg cagattaagg atttggaaat gtttgcctat
     3001 catggtcttt ttcctagtga gaaagaattg gggcagaaat ttgtcgtttc agccatccta
     3061 tcctatgata tgaccaaggc agctacagac ttggatttaa cagcctctgt ccattacgga
     3121 gaattgtgtc agcagtggac gacttggttt caggaaacga gtgaagattt gattgaaacg
     3181 gtagcctata aactggtgga acgtaccttt gagtcttatc ctcttgtcca agaaatgaag
     3241 ttggaactga aaaaaccttg ggcaccggtg catttgtcac tagatacttg ctcggtaacc
     3301 attcatcgcc gcaagcaacg agcctttatc gccctaggaa gcaatatggg agataaacaa
     3361 gtaaacttga agcaagccat tgacaaactg cgagctcgtg gcatccatat tctcaaagag
     3421 tccagtgtct tagcgacgga gccttggggt ggagtggagc aggatagctt tgccaatcaa
     3481 gtggttgagg tggaaacctg gctaccagca caagacttgt tagaaacctt gttagccatt
     3541 gagtcagagc tgggacgggt gagagaagtg cattggggac ctcgtttgat tgatttggac
     3601 ttgctctttg tggaggacca gatcctttat acagacgacc tcatattgcc tcatccttac
     3661 atagcggaac gcctttttgt ccttgagtcc ttacaggaaa ttgcgcctca ttttatccat
     3721 ccgatattaa aacaaccgat ccgcaacttg tatgatgctt tgaaaaaata gaaaaactct
     3781 agttttcagt tacttgcaac tgaaggctag agtttttata ctcttcgaaa atctcttcaa
     3841 accacgtcag cgtcgcctta ccgtactcaa gtacagcttg cggctagctt cctagtttgc
     3901 tctttgattt tcattgagta ttaaaatagg tcattttctt ctgggaggag gatagtttct
     3961 ctaccgtcca tgtctaaaac cagtactctt gggggataac gagggtcgaa aggatggtta
     4021 aagtcaaaat caatggctgt agggaggtgt tgacttgaaa agtggaaggt aatctttcct
     4081 tggttattaa gcaattgaaa ctcgagttct tcttccaatt caaagacatt ttttaagaaa
     4141 tggtcgatga tataccaaaa agagtcaatg atgtcatcag gcaagctggt aacaatgcca
     4201 aaactagcag atcgcatgtg ggtattggta aaagccatat ctctgtcccc tttcttttcc
     4261 cttatcatac agcaaatagg attaaaaatc aagaaaaggt gattttttga aaaggatttt
     4321 agttacaggg agaaataggg aaaaaattcc taaaaatcta ccgaagttaa taggtaaatt
     4381 cccaaattaa cttgattata taactttcag ttactttgag aagttaccga aaaatatttt
     4441 tcatatctat tgacttttag gggtaaaatt tggtatgata gtaggcggta ttgtttaccc
     4501 catttgaaag gccccggaac cttccaaata cttttcgatg ggaaggaaca cccatcaccg
     4561 taaacaaaaa tcgaactata tataggagaa atcatgaaca aaacaacatt tatggctaaa
     4621 ccaggccaag ttgaacgtaa atggtacgta gttgacgcaa ctgatgtacc acttggacgt
     4681 ctttctgcag tagttgctag cgtacttcgc ggaaaaaaca aaccaacatt tacaccacac
     4741 actgatacag gtgacttcgt gattgttatc aatgctgaaa aagttaaatt gactggtaaa
     4801 aaagcaactg ataaaatcta ctacactcac tcaaaccacc caggtggatt gaaacaaatc
     4861 tctgcaggtg aacttcgttc taaaaatgca gtacgtttga ttgagaaatc agttaaaggt
     4921 atgcttccac acaatacact tggacgcgct caaggtatga agttgaaagt atttgttgga
     4981 gctgagcaca ctcacgctgc acaacaacca gaagttcttg acatttcagg acttatctaa
     5041 ggaaaggaac aataaagtat gtcacaagca caatatgcag gtactggacg tcgtaaaaac
     5101 gctgttgcac gcgttcgcct tgttccagga actggtaaaa tcactgttaa caaaaaagat
     5161 gttgaagagt acatcccaca cgctgacctt cgtcttgtca tcaaccaacc attcgcagtt
     5221 acttcaactg taggttcata cgacgttttc gttaacgtta taggtggtgg atacgctggt
     5281 caatcaggag ctatccgtca cggtatcgct cgtgcccttc ttcaagtaga cccagacttc
     5341 cgcgattcat tgaaacgcgc aggacttctt acacgtgact cacgtaaagt tgaacgtaag
     5401 aaaccaggtc ttaagaaagc tcgtaaagca tcacaattta gtaaacgtta attcgaaaga
     5461 attactatac ttatacagag cacctttcgg ggtgttcttt ttttatactt tcttactaaa
     5521 ttggtgcaat tgacacagtt gttgcgactt tagtcgctta caaatgtgac tgcaacctga
     5581 catggtcagt tgcctcaaaa cgttaatcaa tacgattata tcaacgtttc aaagcactca
     5641 aaagtttacc ttatgggtgc ttttttcgtg tttttttgaa aagtttacct tatttttata
     5701 gtaaggtgaa ataagaatag gacaaaccga ggatagtcaa atcgaattct aacaatgttt
     5761 tagaagtaga ggtgtactat tctagtttca atctactata ttatggataa tgcaagattc
     5821 catagaatgg ggaagctaga actcttgtgt gaagagttta ggcgtaaact tttacctctt
     5881 cctccctact cacctgagta caatcctatt gagaaaacat gggctctttt gtcctgctct
     5941 tgtttcaatt gactataaat cgctgtaaga aaaaatcatt ttattggtat aaagaagtga
     6001 ttgaaagtaa tggcgaaacg ttagataatt agttcataaa aaacaagttt agctcctcat
     6061 ctaaacttgt ttttttaaaa ttgaaggatg attttggagg atataagatg acattgacta
     6121 ttcatgtata ttataaaggt gatggagata gtgcaataaa gtttgcaaga gaaatgcttg
     6181 atagtggcct tgttgaagaa attagaaatc aaaaaggtaa cttgaaatac gaatattttt
     6241 taccaattga aaaagaggga actattctcc ttattgatca gtggataaat caagaaactt
     6301 tagataagca tcatcaatca aagacaatgc agaaaatctt agatttaaga aaaaaatatc
     6361 acttgcagat gcaagttgaa cgttatatag aagatgattc tgggatgcca gaaagcgata
     6421 agtcgtttat agacacaggg aattagaatt attgtgagag tttttgtagt ttgaaagtat
     6481 tgaaaatcgt aaagatatca ttacgagaat acaagaagat attgttctat ttccattaaa
     6541 aattacttct ttccttaaca ataaggaaaa agaatgcgtt ttcatctttt ggtttgaggt
     6601 ttataataag agtatcaaag tcaggaggaa aaaagatgac tacttttaaa gatggatttt
     6661 tatggggtgg tgctgttgct gctcatcaac ttgaaggtgg atggcaagaa ggtggcaagg
     6721 gaattagtgt tgctgatgtt atgactgctg gtcgtcatgg agtagctcgt gaaattactt
     6781 tgggagtttt agagggtaaa tattatccaa atcatgaggc gatagatttt tatcatcgtt
     6841 ataaagaaga catagcactt tttgctgaaa tgggattcaa gtgcttccgt acctctattg
     6901 catggacacg tatctttcca aaaggtgatg agttagagcc gaatgaagaa ggattacagt
     6961 tttatgataa tctttttgat gaatgcttaa agaatggtat tgaacctgtt atcactctat
     7021 ctcattttga aatgccttat cacttagtga ccgaatatgg tggttggaaa aataggaaat
     7081 tgattgattt ctttgctcgt tttgcagaag tcatatttaa gcgttacaaa gataaggtta
     7141 aatattggat gactttcaat gaaatcaata atcaagcgaa ttatcaggaa gattttgcac
     7201 catttactaa ctcaggtatt gtatatgagg aaggtgagaa tagagaggca attatgtatc
     7261 aagcagcaca ttacgaatta gttgcttctg cacgagctgt aaaaattggt catgagatta
     7321 atccagattt tcaaataggt tgtatgattg cgatgtgtcc aatttatcca gctacttgca
     7381 atcctaagga tatcttaatg gcaatgaaag ctatgcaaaa gcgttattat tttgctgatg
     7441 tgcatgtttt aggtaaatat cctgagcata ttttcaagta ttgggaacga aaaggtattt
     7501 cagttgattt tactgcccag gataaagaag atttacttgg tgggactgta gattacattg
     7561 gtttcagtta ctatatgtcc tttgctatcg actctcatcg tgaaaataat ccttattttg
     7621 attatcttga aacagaagat ttagtgaaaa ataattatgt taaggcttct gaatgggagt
     7681 ggcaaattga tccagaaggt ttgcgttatg cgttaaattg gtttacagac cactatcact
     7741 taccactctt tattgttgaa aatggttttg gagctataga tcaagttgca gcagatggta
     7801 tggtacatga tgattataga attgaatatc taggtgccca tattcgtgaa atgaaaaagg
     7861 ctgtagttga agatggtgtt gatttaatgg gttatactcc atggggatgt attgatttgg
     7921 tttcagctgg taccggtgaa atgcggaaac gttatggctt tatttatgta gataaagatg
     7981 ataatgggaa gggaagttat aatcgttccc cgaaaaaatc ttttggctgg tataaggaag
     8041 ttatttcatc taacggtgaa tcagtagaat agtataaaca aactgagatt attctcagtt
     8101 tgtttagcat attgaggtga catatgattg gaagtattgc agcaatttta acaacatttg
     8161 catttttacc acaagttttt cgtgtagtta agacaaagga cacaggatct attgctctag
     8221 gtatgtatgt tatgcaagtt attggtattg cattgtggct tgctcatggt attcgtatag
     8281 gagacttgcc tttgatttta gcaaatagtg tttcatttct actctcagga ataattttat
     8341 tttataaatt gaagtataaa taattttttt ccatatcatt taggaaaatg agggaattgt
     8401 cattggaaac gctttatttt ataataaaaa tagataacaa ataagccaac ttagcacagc
     8461 tttgattgga tttaagaaaa ggagaaaata ttatggcaaa agctttaatt atttgtgcag
     8521 caggaatgtc ttcatcactt atggcacaga aaacaacaga gtttctaaaa ggaaaaggga
     8581 aagatattgt agttgatgct attagtgcta ctgaaggagg gaaagctatc gcagcagcag
     8641 cttttgattt gtatcttgtc agccctcaaa caaaaatgta ctttaagcaa tttgaagagg
     8701 ctggttctaa agcaggaaaa ccagttgtac aaatacctcc tcaagcctat attcctattc
     8761 caatgggaat tgaaaaaatg gctaatttga ttcttgagaa tatttgatga acacatgaaa
     8821 tgtcaataaa tatttaagtt aagataaatt gttttgagtt tgatataaat tgatttaagt
     8881 gtataatatg cttcaatata tgaaaacata cgttttagga gggaatgaat gtgttaaaca
     8941 ttaaagagaa agatatcctt caatttttaa taaaaaataa ggaacgtttt gttacaagca
     9001 aagaactggc agaatattta tcctgttcag atagaactgt ccgtaatgta ctgaaattaa
     9061 ttgagaagac aatgattatt caaggagtcc ggttgatttc aaaacaaggt caagggtatc
     9121 aaatattttt tgagaatcaa ggcgcttacc aggagtttag acaaacttat gaactggaag
     9181 aagactatac gaaaactgcg gtttcaaaag gtgatgatcg tttagtattt atcttaaata
     9241 aattattatt tgagcaagtt ccagttcttt ttgatgattt agctgatgaa ctttacgtta
     9301 gtcgttcaac attatctcat gatttcagaa agatacgagt gatgttatca gagtataatt
     9361 tgtccattga gagtagggcg aataaaggag tttatgtctc aggcgaggaa cgtgataaac
     9421 gacgttttat cataaattat ttcctagaga atcaattttt taaaacaata cattgctatg
     9481 taaaatttaa cttttttgct caaactgttc cattagaaga atttgctaga attgttttgg
     9541 atgaatgcca agaggccaac ctaaaactat ctgattttgt actacagaat ttagtagttc
     9601 acatagcatt gtctgtgatt cgtttaaaat ccggatttga aataaaaaat atagactgtc
     9661 agatgactga tgatgcaata gagagaaagg ttgctcaaag aattctttct aaagtaagag
     9721 aagtaacaaa tcaggaattt ccagttcagg aaatagacta cattactttg catttgttag
     9781 ctaagagtca acaatgtcaa aaaaatcaga agaatatttc tgaagaagtt ttaaaaaaat
     9841 ctttatttaa aacatttcag aatttaggat tagatgatat gtataacttt tcttcagatt
     9901 tccaactaat tgaaggattg ataactcatc taatgacact acaagtacgt ttagaaagcc
     9961 ggattacctt aaataaccct ttggtagatg aaattaaaca gaattatagt gatatattct
    10021 ttatgactag agaaattctt gcaaatatgg acaagttctt ggagtggaca atatcagatg
    10081 atgaagtagc atatgtaagt cttcactttt tagctgctat ggaacgtagc aaagagagta
    10141 ctaagtttaa tattcttgct atttgtgcaa caggatttgg tgcagcccaa atgttaagaa
    10201 atcgtttgga aacggagttt gggaagcgag tagaagttgt tgatgtaata ggctattatg
    10261 aattgaatca agaaaaactt aaaggaatag attttatagt gtcagctgtt gatttatcaa
    10321 atctatattt tcaaattcca gtttttaaag ttagtgtgtt tcttaagagt gatgagatgg
    10381 aaatgattag aaaagctatg gatcagatgc aagtttctag ttatgtacaa tcatcaaaga
    10441 ttaataaatt tgaaaacaac agctttagac agtattttag taaagaaaat tttttaatat
    10501 gtactgaatc agacaaggta aatttacttg aaaagatggt tgagagttta tctgttggtg
    10561 aatcaaatga gtttgaacaa tctttacttt atggaattaa acagcgcgaa gaattaagtt
    10621 cagttgtttt ttctgaaaaa attgcagttc cgcatcctat tcagcccttt ggtacagagg
    10681 gaaaggtttc ggttgcaatt tgtaaagatt cgttactctg ggataatcaa agttcatatg
    10741 tccagctagt atttcttcta tctccatcaa tatatgggaa cgaaggtttg gcaactgtga
    10801 ctaaaaagat agtctctctg acagagaatg atgaattgca aaatcagcta atatcttgca
    10861 ataactttga agattttatt aacatatttg agaagataaa ataggaggtt catatgaatc
    10921 aagaggaact gcaggttgca gcatttgaaa ttattctgca ttcgggaaat gcaaggtcag
    10981 aaatacatga agcatttgca aaaatgagag aaggtagttt tgatgatgca gaaagtaaat
    11041 taaatcaatc aaatgagata attcttgaag cgcatcatgc acaaaccaaa ctcttgcaag
    11101 aatacgcaag tggagtggaa attaaaattg aaattatcat ggtacatgct caagaccatt
    11161 taatgaccac tatgacatta ttagaagtag caaaggaaat gttagctctt tataagaaag
    11221 ttaactaaat atgtgttgtt atataaggag gaaatgatat ggataaaaaa gaacgacaaa
    11281 aaattgaaca acaacgtcga gagatggctc taactaatac tttttttaat cgatatcttc
    11341 ttctacgtta ttcaatagca ctcttctttt ttgggaatat ttattggctt ttgaatcagt
    11401 ttattagtcc ctcgcctatt attattttcc ccattatgct catagtattt tctattttag
    11461 caactattga acaatttaaa ctttatggaa ataggaaaga aaagttggga ataacactaa
    11521 tgtttgttag aatacaaatg cttatttcaa taggattact tgttttaact tggacaagtt
    11581 ggtttgagaa tctttttccg atttttgaaa ataaccaagt agcacgttta tttgtttttg
    11641 tagttcttct attaggttta gttctgagtt tgttagacat tagaagaatt aaaaaaattt
    11701 ataaacgaac agataaggct tatcaacaat ttgtccaatt ggaaaagaac tcacttagtt
    11761 tataatcctg aactagtata atttgtattt gaaactagtc tttaaaaagt aaaaattatt
    11821 atgttttatt aaggaggtct atttatggat caacaaaacg ggttgtttgg ttttcttgaa
    11881 aaccatgtta tgggaccaat gggcaaactt gctcagttta aagtagtgcg tgctatcacg
    11941 gctgcaggta tggctgctgt accatttact attgtaggat caatgttttt ggtattcagt
    12001 attttgccac aagctttctc attttggcca attgtggcag atattttctc tgcttcattt
    12061 gataaattca catcacttta catggttgca aactatgcga ctatgggttc tctatctctt
    12121 tatttcgttc tatcacttgc atatgaattg acaaaaattt atgcagagga ggaagaactc
    12181 aatatgaatt ctcttaatgg tgccttgctc gccttgatgg cttttgtcat gacagtaccg
    12241 caaatcattt ttgatggtgg aatgatgaag actgtgacaa gtctaaaaga aggtgcagta
    12301 attgcagatg gatgggcaat gggaaatgga gtcgcacgtt ttggtacaac aggaattttt
    12361 accgcaatca ttatggcaat tgtgactgtt cttatttatc gtatgtgtgt taaacataat
    12421 tgggttatta aaatgcctga agctgttcca gaaggagttt ctcgtggatt taccgctttg
    12481 gttccgggat ttgttgttgc atttgttgtt atctttatca acggtcttct tgtagcaatg
    12541 ggaacagata tttttaaagt cattgcaatt ccatttggtt ttgtatccaa tctgactaat
    12601 tcgtggattg gtttaatgat tatttatcta ttgactcaac tactttggat tgtaggtatc
    12661 cacggtgcga acattgtttt tgcatttgtt agtccaattg ctcttgctaa catggctgaa
    12721 aatgctgctg gcgggcactt cgctgttgca ggtgaatttt ctaatatgtt tgtaattgca
    12781 ggtggttctg gtgcaacttt aggactatgt ttatatattg cttttgcctc taaatctgaa
    12841 cagcttaaag caataggacg agcatctgta gttccagcct tatttaatat taatgaacca
    12901 ttaatttttg gattacctat tatctataat ccagccttgg ctataccatt tattttagca
    12961 ccaatggtta ctgctactat ttattacgta gcgaattctc taaactttat taagccaatt
    13021 atcgcacagg ttccatggcc aactccagta gggattggag ctttcttagg gacagcagat
    13081 cttcgagctg tattagttgc tctagtatgt gcatttgcag cattcctagt ctatcttcca
    13141 ttcatccgtg tatatgatca aaaattggtg aaagaagagc aaggtatcta ataagtaaaa
    13201 aagtatactc gatgatagaa tccatatagt ttaaataatg agcgttaaca ttagcagtga
    13261 agaggtcttt ttaagatatt cttctctgct ttttcatatt ttttcgctat gtagagtatg
    13321 aagcaaaata gaaagtagtt cgaatagttg ataaacaggg aattgctgtt atttaaaaaa
    13381 aacaataaag taaagtatgt tatcgtagaa tacgatacaa tagtaaaaaa gcaaaaagaa
    13441 tgcgttttat atttgggttt gaggcttata aaataggata agcgtactga aaacttagat
    13501 atgacaaaca aaataaaatt tttaaaagct acagagaaac atggtataat gtatctaaag
    13561 gaggtagcat atgtccttag atatagataa agaaaagatg acaatcatgg gaatagcttt
    13621 tgaaaaccga tccgttttta agagtgtttg gtatgcttta agtacaaata tgattgaagg
    13681 atggcgacct acggttagtg atgttgaaaa attacgagat gaagctcttt ctctggggat
    13741 gacttaatga agcgcaactg ttatgagtct tatgactatg ttgatccgga taatttatat
    13801 accgatccgg gttcttcagt tctaagaaat aagcaggaag gaaaaaagca cgcgagcttg
    13861 aatatcgtat gattgctagt aagagtttga aattatttat caatcctatc ttagttaata
    13921 gtttaaaagt agtttgtttc acaactgcat aagcttagaa aagttgaaaa gtgataactc
    13981 tttaataaac tcttggtttg ttaaaagata ttttgtccac tacatttttt tcaattgact
    14041 attatttgtc atagatataa cagatagaac attgcattca gaataatgaa aaacgcatca
    14101 aaaatggtgc gttctgtttt tatgctacca taaaaggagg aaaacaaatt gaaaattttt
    14161 aagggagagt tttatcgaat ctctgtatta acagacaagc tagtaaggtt agaatactct
    14221 cgaactggaa attttgagga tagaacgaca caacttatct ataatagaga ttttggccaa
    14281 gtttcgttag attatatcga gacatcaaat gtactagata ttatgacgga ctattttcat
    14341 ctgcacttta ataaaggaga atttaacgcc gaaaatttat ttatagaatt aaaaggaaat
    14401 tttgccgtat atggtagtcg ctggtatttt ggtgaatcta ttgaaacgtt aaaaggaaca
    14461 gctcggactc tggataaggc agatggagca atctcgttag aagatggaat tattagccga
    14521 aatggtatag ccttattgga tgattctcaa ggatttattt gggatgaaca atctggttat
    14581 attgagagag aaaatcaaat tgacctgtat ttctttgcct atgggcatga ttatagagga
    14641 gcaatcagag acttttacca tttgactggt tcaacaccct tgttgccaag atatgcttta
    14701 ggcaattggt ggagtagata ttggccttat acgtcggatg aatacttgga tttaatagac
    14761 agatttgaaa cagagaaaat tccattatct atcggtgtgt tagatatgga ttggcatata
    14821 actgacattc cagctcgttt tggaagtggc tggacaggat atagttggaa tagaaactta
    14881 ataccaaatc cagaacagtt attgcaacaa cttcatgata gaaagttaaa actctcctta
    14941 aatgtccatc ctgctgatgg gatacgggct tatgaagaag cttatcctca aatcgcaaaa
    15001 cggttggggt taaatgtaga actagaagaa cctgctattt ttgatttttt taatccctct
    15061 tttagggaag cctactttaa agatgttcat tatgagctag aaaagcaggg agtagatttt
    15121 tggtggattg actggcaaca agggacacaa ggtatgctag atccactttg gcttttaaac
    15181 cattatcact atcaggatag ttgtaaaaat gcagaaggtg gtttgatttt atcaagatat
    15241 gcaggtcctg gtagtcaccg ctaccctgtt ggtttttcag gggacactat tattagttgg
    15301 aattccttaa gatttcaacc ctattttaca gcgacagcat ctaatatcgg ttatagttgg
    15361 tggagtcatg atatcggtgg acatatgttg ggggattatg acgaagagct acaaactaga
    15421 tggctacagt ttggtgtttt tagtccgatt actcgattac atagttctag aagtcctttt
    15481 aatagtaaag aaccttggtt cttttcagaa acaacatcta agattatgaa gaaatacctt
    15541 cgtttgagac atcagatgat tccctatcta tataccatga atgtaaagac acatgaggaa
    15601 ggtgccccat taatcagtcc aatctattat ttctatccag agaatgatga gagctataat
    15661 gttccaaacc aatacttttt tggaacagaa ctgatggtgg ctcccattgt agaaaagatg
    15721 gatttgacat tccaatctgc aaaagtagat gtatggttcc ctgaagatga atggtatgac
    15781 ttcttttcag agaaaaaata cacaggtggt gtgaagttaa gtgtttatag ggacatctcg
    15841 actacgcctg tgtttgcaaa aagtggtgca atcattccct tggttggttc tgagataggt
    15901 atgggtgttg atttacctga ggttgtagat tggtatgtat tcccagggaa acaacattct
    15961 tttgaaatgc ttgaagatca aaatggtcaa agatataaaa caagattatc aatcgattgg
    16021 gaaatgggaa tggtagagtt agcattacaa ggagattcta gtatcgttcc aagcaataga
    16081 aaacatagaa ttcattttaa aggaacgaat gtgtctataa ttgaattgcc aaataagaat
    16141 gatacagcta aatttgaatg gaaagataat aaaaggacat ctttaaatga cgaagttttt
    16201 agactactaa agacagcttc tcttccatat gaattaaaag atagattgtt aaatcaattc
    16261 atcaatgcga aaaactctca tgacttaatg aatatcttgc atcatcagga taaggaattg
    16321 agagggcgtt tgttggaaat gatatttact agccaaaact aattgaaata attagaaagt
    16381 catttgttta gtgtatatga agattctgta tggtattcgt tatcactggt cacattgaaa
    16441 atgtgaaata agcacctaca acatagactt tacaacactt catgttttat atcatggggt
    16501 gtttttctat ttaagtcaaa gcccatcaaa aaatctcact attcatcagg ttaaaggaaa
    16561 cctaatcata gtgagattgt gaattatagt agagtttgta tcgcctcttc aatttgtttt
    16621 gggtttgttt ttgaagaaaa gcgttcaaag acttgcccat cacgaccaat gagaaactta
    16681 gcgaaattcc attcgattcg ttttcctagt gggccagatt tatggtcttt taaccagaca
    16741 tagagagggt ctgcttcctt accgttgacc ttaatcttgg caaaacgtgg gaaggtggtt
    16801 tgaaaatgta ggctacagaa ggcgttgatt tcctctgcgc tgccgggtgc ttgtcccata
    16861 aactgattgc aagggaaatc caatatttca aagccctgat cttgatagcg ttcatagagt
    16921 tcttgaagtc cctggtactg gggcgttaaa ccacatccag tagcagtgtt gacaatcaag
    16981 agaactttac cacgatagct atccaaggga gttgcttggt tgttttggtt caaaacggaa
    17041 aaatcataaa gtgaagtcat gagggcctct tttctttttc tacattgtaa cagtttttac
    17101 ttttttcgtc gagtcggatg gaagatattg ttgaagaata agccttcaat atgatagaaa
    17161 ccgtttacaa ataactttaa aaatggtatg atttaattaa accggtttac tttgttgcta
    17221 taaatctcta atttgagatt tagttcctat tgattttaca atatgtttat tggagtgtat
    17281 acatgcaaac aaaaacaaag aagctcattg tgagtttgtc ttcacttgtt ttatcaggat
    17341 ttttattaaa ccattatatg acagttggag cggaagaaac gactacgaat accattcagc
    17401 aaagccagaa ggaagttcag tatcagcaaa gggatacaaa aaatttagtt gaaaatggtg
    17461 attttggtca gacggaggac ggaagcagtc cgtggacagg aagcaaagct caggggtggt
    17521 caacttgggt agaccagaag aatagttctg cagatgcctc aactcgagtc attgaggcta
    17581 aggatggggc tatcactatc tcaagccatg agaaattaag ggcagcgctt caccgtatgg
    17641 ttcctattga agctaagaaa aagtataaac tgcgtttcaa gattaaaaca gatcataaag
    17701 tcgggattgc caaagttcgt atcattgagg aaagtggtaa ggacaagcga ttgtggaatt
    17761 ctgcaacgac gtcaggaaca aaggactggc agaccattga agcagactat agcccgactt
    17821 tagatgttga taaaatcaag ctggagttat tctatgaaac aggaactggg actgtttcct
    17881 ttaaggatat tgagctggta gaggtagcag accagccttc tgaggattct caaacagata
    17941 aacagcttga ggaaaagatt gatttaccaa ttggaaaaaa acatgttttt tctcttgcgg
    18001 actatactta taaggtagaa aatcctgaca ttgcttcagt caaaaatgga attttagaac
    18061 ctcttaagga agggacaacc aatgtcattg tcagtaaaga tggcaaggaa gtgaaaaaga
    18121 ttcctttgaa gattctagcc tctgttaagg atgcatacac agaccgtttg gatgactgga
    18181 atggcatcat cgctgggaat caatactatg attctaaaaa tgaacagatg gccaaattaa
    18241 accaggaatt ggaaggaaag gtagctgata gcctatctag tatttcaagt caggcggacc
    18301 gcacctattt gtgggaaaaa ttttcaaatt ataaaatgtc tgcaaatctg actgccactt
    18361 atcggaaatt ggaggagatg gccaagcaag tgaccaatcc ttcttctcgt tattatcaag
    18421 atgaaactgt cgttcgaaca gtcagggatt ccatggaatg gatgcataaa catgtctaca
    18481 atagtgaaaa gagcattgtt gggaactggt gggattatga aatcggtaca cctcgtgcca
    18541 tcaacaatac cttgtctctg atgaaagaat acttctctga tgaggaaatt aaaaaatata
    18601 cagatgtgat tgaaaaattt gtaccagatc ccgaacattt ccgaaagacg actgataacc
    18661 cattcaaggc tctaggtgga aacttagttg atatgggaag ggtaaaagta atagctggtt
    18721 tactgcgtaa ggatgatcaa gaaatttctt ctaccattcg ctcgattgag caagtgttca
    18781 agttggtaga ccaaggtgaa ggtttttatc aagatggatc ctatatcgac cacaccaatg
    18841 ttgcctatac gggtgcttat gggaatgttt tgattgatgg cctgtctcaa ctgttgccag
    18901 tcattcaaaa gaccaagaat ccaatcgata aagataaaat gcaaaccatg taccactgga
    18961 ttgataaatc gtttgctcct ttgctggtga atggagagct gatggatatg agtcgtggac
    19021 gctcgatcag tcgtgcaaat agcgaggggc acgtggccgc agtagaagta ctaagaggga
    19081 ttcaccgaat agcggatatg tctgaaggag aaaccaaaca acgtttgcag agtcttgtga
    19141 agaccattgt tcaatcggat agttattatg atgtctttaa gaatttgaag acttataagg
    19201 atatcagttt gatgcaatcc ttgttaagtg atgcaggagt cgcaagtgtt ccaagaacaa
    19261 gttacctatc tgcctttaac aagatggata aaacagccat gtacaatgca gagaaagggt
    19321 ttggatttgg cttgtcactc ttttccagcc gtaccttgaa ttacgaacac atgaacaagg
    19381 aaaataaaag tggttggtat acgagtgatg ggatgttcta tctttacaat ggcgatttga
    19441 gtcactatag cgatggctac tggccaacag ttaatccata taagatgcct ggtacaacag
    19501 agacggatgc taagagagcg gatagcgata caggtaaagt tttaccgtct gctttcgttg
    19561 gaacgagcaa actagatgat gccaatgcga cagcaaccat ggatttcacc aactggaatc
    19621 aaacattgac tgctcataag agctggttta tgctaaagga taagattgcc tttttaggaa
    19681 gcaatatcca aaacacttca acagatactg ctgcaactac aattgaccag agaaaactgg
    19741 aatcaagtaa tccatataaa gtctatgtca atgataaaga agcctccctt acagaacaag
    19801 aaaaggatta tcctgaaacc caaagtgtct ttttagaatc gtccgattcg aaaaagaata
    19861 ttggttactt tttctttaag aagagttcaa tcagtatgag taaggctttg caaaagggag
    19921 cctggaagga tatcaatgaa ggacagtcag acaaggaagt tgaaaatgaa tttcttacta
    19981 ttagtcaggc tcataagcaa aatggagatt cttatggcta tatgctcatt cctaacgtgg
    20041 atcgtgccac cttcaatcaa atgataaaag agttagaaag tagcctcatc gaaaataacg
    20101 aaacccttca gtctgtttat gatgctaaac aaggagtttg gggcattgtg aaatatgatg
    20161 attctgtctc tactatttcc aaccaattcc aagttttgaa acgtggagtc tataccattc
    20221 gaaaagaagg ggatgaatat aagattgcct actataatcc tgaaacccag gaatcagctc
    20281 cagatcagga agtctttaaa aagctagagc aagcagctca gccacaagta cagaattcaa
    20341 aagaaaagga aaaatctgaa gaagaaaaga accattcgga tcaaaagaat ctccctcaga
    20401 caggagaagg tcagtcaatc ttggcaagtc tagggttctt gctacttggg gcgttttatt
    20461 tattccgtag aggaaagaac aactaatttg ttcatcatct agatgaatta catgaaactg
    20521 tggaaaatag aaaaaatccg aagaaggcca aatatcttcg gatttttctt ttaaaaaggg
    20581 ataagttgat acttatcctt ttttacttca ttatgtgact atagcgtttt gaaactagaa
    20641 taggacgtca taactgctaa aagatttcta taaattcatt tgattttcct aatcaatttg
    20701 ttcgtatcct atttcactcc actatattag tccgtctcgc tccgtgaggt gcgagacaaa
    20761 acaaccaccc gctatgcggg tgcgcgtcga aggctatacc aaaaaactcc aaacgcgata
    20821 caataaaggt gttcaatcca attgtaaagc gaaaggagaa aaaatatggc acaaaagact
    20881 catagtttat catacacaaa gtggatgtgt tctatcatat tgtgttcacc cctaagtata
    20941 gacgaaaagt catctataat caatatagaa gtagtttagg cgaaatattt catcgcttgt
    21001 gtggttataa aggtgttgaa atgatcgagg gtcacttaat gccagaccat gtacacatgt
    21061 tagtcagtat tccactaagg ctaagtgttt cgagtttcat ggggtattta aaaggcaaaa
    21121 gtgcactcat gatgtttgac aaacacgcca atctcaagta caagtttggg aatcggcatt
    21181 tctgggcgga aggttattat gtaagtacag tagggcttaa taaagccaca cttaagaaat
    21241 atagtcaaga ttaaagaaat tatcgagtgg atgatttctt cacgagtatg aaaatttgag
    21301 aacgagtaaa gtatgatata tagtaaaatg aaataagaac aggacaaaat cgatcaggac
    21361 agtcaaatcg atttttaaca atgttttaga agtagaggtg tactattcca gtttcaatct
    21421 actatagcac tagataaatt gagtgtaaaa gaatatgagg atccctttag ggatagtggt
    21481 aagtaatacc aaagtctctt aaagaggcaa gtgacgagtc aagagcaata aggcttgaac
    21541 aacgtgaaag ccagcgtctt taggcgctgg ctggtaattt gggcttatag ctctgggata
    21601 aaccacccgt ttgacgggtg gttatgattt tatctgagtg taacatactg ttgggcaatc
    21661 tcgctgatgc ggtcaaagtt gccttgggaa gcgagtttat tgagttcgcc accaattcca
    21721 acggcgtctg caccagcagc gaaccattga gggatgttgt ttagaccgac tcctccggtt
    21781 accattacgg aaacttgtgg gatcggtgcc ttgactgcag agatatatgc tggactgaga
    21841 gcactacctg ggaagagttt gatgatttca ctaccggctt caagtgcagt cgtgatctct
    21901 gtgagggtaa tacagcctgg aatgtacggt gtgctgtaga gattgcacat tttcgcagtt
    21961 tcagcatgga aagatggaga aacaacgtaa tttgctccgg ctagaatggc atctctagca
    22021 gttacggcat caagcacagt acctgcaccg atacaaacac tctgatcgtc ctgatacaag
    22081 tctacaagtt ccttgatgat ttgtcctgca tactgattgg tataggcgat ttcaatagct
    22141 ttgataccgc ccttgataca agcaatcgag gcttgcagtc cttcttcctt tgtatttcct
    22201 cgaatgacag cgacaatttt ctgttttttt agttcaataa tcgtatctga tttggtcatg
    22261 taattctcct aacgaatgat atcttgtgca tttgccagta aattttcaat actagttgcg
    22321 gaagtggaga gatggtctcc tggaagagtg catttgagag ttgcgctcgc aactgcaaag
    22381 tcaatggtag tttttaggga ggaatgatgg agtagttggt aaagggcacc agatataaag
    22441 gcatccccgc taccaattcg ttgatagact gcagttttta gttggacaga ctcttcaaat
    22501 cgttcttcca gagcataggc ttgatagaca tttttgtctt gctcatcact agagcggagg
    22561 gtatggaaaa tggccttgaa accataggct tcttttaaaa gtcgcatgcg attttccacc
    22621 tcttctaggc tagcactgtc tcttggaaac atctctagat tttggtcatc aatcatgaga
    22681 ggatcaatac cgaagcaata gtcagtaaaa cgtgcaaact tagaaaattc atacttggct
    22741 tctaggactg aaatcatctt tgttctcaga ttgagatcca ttgaaacgac aattcctcgg
    22801 cgcttggctt cttccaagag taggagaagg atcgcacgga cctcttgacc gatagctacg
    22861 gtgattccac taaaatgaaa atggctaatc ccctgaaaga gagaatccat atctagcatg
    22921 tttggccgaa tctggctgat actcgtatgc ttacgatcgt agaaaacttc actttgacga
    22981 caaccaaagc cgttctccaa atagtagagg ccgattcgat cgccaagccg acagattgaa
    23041 ctggtatcga tttggtgctg tttcaagaat gtgagaaaac gatctccaat ctcgttggca
    23101 gggagtgcgg taaagacttt cgttgagata cccagggctt gcaaattaca agcgatgtta
    23161 atttctgatc cgccaaaata agtcgaactg gcaacatgat cgccgatact ggtggcatct
    23221 aatggtgaga ttcgaattaa tggttcgcca aacagtaata ttttcgtcat ccattagttc
    23281 ctttctggtt tagaatagct ttacttggct aatacttctt tcaaataagc agcgacttca
    23341 tcatcttggc agttttcaaa gaagtattct tggaaatatt tgccagagat ggtgtctttg
    23401 aggaagtctt ggtcgattgt tttaaggatg gtcatcaaat cattgtgggt gatttgtttc
    23461 acctcgttta agatacgagc gttgcgttgt tcagggatta cacgttctct tgggtagcca
    23521 ccgcccattt cttcagcaaa taagcgttca aacatcaatt tgagggtcag ttctgccccc
    23581 cagccaaatc ctttggcata agggatagac aaggcgttac caccattgat ttgagaataa
    23641 aggtaagcgt cagttgggtc cactgctaga ccacagacaa caccagggaa gctgtttaaa
    23701 gcaagcatag cccctacacc cgtaccacag ccggtaacaa caaagtcaac tgcctttgta
    23761 tttaaaagga tggcagccat tagtccgttc tgcacataag ttaattgact ttctccttct
    23821 tctccacgca taccatagtt aaataattgg tagccttttt tatctgtcgc ttcttttaga
    23881 ctatcgtaaa taatgtgatt cttgctagct tgactatttt cattgattaa tgcgattttc
    23941 ataagatctt ctttctattt ttactcaggt tgttttccga tgtaggctaa gataccgcca
    24001 tctacatata ggatgtggcc attgacaaaa ttgctggcat cactagcgag aaagacagca
    24061 gggcccatca aatcttcagg atttccccaa cgtgcagcag gtgtttttgc aatgatgaac
    24121 tggtcaaatg ggtggcgaga accatcttct tgcaactcac gaagaggtgc tgtttgagga
    24181 gtggcaatat aacccggtcc aattccgtta cattggatat tggctccacc gtattcagac
    24241 gcaatgttgc gggtcaacat tttcaagccc cctttagcag cagcataagc gctaactgtt
    24301 tcacgtccca gttcgctcat catcgaacaa atattgataa tctttccatg ccctttcttt
    24361 atcatagaag gaataactgc ctttgaaacg ataaatggtg cgtttaagtc aatatcgatg
    24421 accttacgga aatcagcggc gctcatttcg cacattggaa ctcggcggat aataccagcg
    24481 ttattaacga ggatgtcaat gacaccaacc tcttgttcga tttgcttgac catggcttgg
    24541 ataccgtcct cgtctgtcac gtcacagaca tatccatggg cttcgatgcc aacttcacga
    24601 taagcttcaa tccctttatt gaccagatct tgattgatat cgttaaagac aatagtagcg
    24661 ccggcttcag cgtaggattt ggcaatagca aatccaattc cataagaagc gccggtgatg
    24721 agagcaattt ttccttttaa agaaaactgc tcaattgaga atgatgtatt tgtcatttga
    24781 tacttccttt caaatttgtt aagacttctc tataaccaaa ttatattcta gtaaaccggt
    24841 tttgtcaata aataaatatt tttatttata aaaagattta ttttccttaa aaatggaata
    24901 ttttgaaatt tctgtctatt aaaatgatta aaaagataat gaaagcgcta atattttttt
    24961 aaaaaaacta ttgacaagat aaaaataaag gattacaatg gggaatataa agtaaaccgg
    25021 taaacctaaa caagaaagga gaaaagatga aaattgtact tgtagggcat ggacattttg
    25081 ctacagggat ttatagttct ttacaattga ttgcaggcaa tcaagaaaat gtggaggcaa
    25141 ttgactttgt ggaaggaatg tcagcagatg aactcaagca aaaaatctta cttgcaattt
    25201 caaatgaaga agaagtttta atcctaagtg atctcttggg aggatcgcca ttcaaggttt
    25261 cttctaccat aatgggagaa aatccagcca aaacaatgaa tgttctctcg ggtttgaact
    25321 tagccatgtt aatggaagca gtctttgcta gaatggctca tagctttgat gaggttgtta
    25381 ataaatcagt agtggcggcc cagggcggag tcgtaaatgg taaagaattg ttttcaacgg
    25441 atgcagagga agaggaagaa gatttcgaat cgggtattta aagggtaaaa gaatgataaa
    25501 aaaggttacg attgaaaaaa taaaatcgcc tgagcgcttc ttagaagtac cacttctgac
    25561 gaaagaagaa gtcggccagg caatcgataa ggttattcgg cagttagaac tcaaccttga
    25621 ctatttcaag gaagatttcc cgacgccagc tacctttgat aatgtctatc caatcatgga
    25681 taacacggaa tggaccaatg gtttctggac aggagaactg tggttggctt atgaatacag
    25741 tcaacaggat gcatttaaaa acatcgctca taaaaatgtt ctttctttcc tggatcgtgt
    25801 caataagaga gtagaattgg atcaccatga tctcggcttc ttgtacacac cgtcttgtat
    25861 ggctgaatat aagataaatg gagatggaga ggctagagaa gcaaccttga aagctgcaga
    25921 taagttgatt gaacgctatc aagaaaaagg tggttttatt caagcttggg gagacttggg
    25981 caagaaagag cattaccgtt tgattatcga ctgcttgctc aatatccaac tcttattctt
    26041 tgcttatcaa gaaacaggcg atcaaaaata ctacgatatt gcagaaagtc atttctatgc
    26101 ttcagctaat aatgtaatcc gtgatgacgc ttcgtccttc cacaccttct attttgatcc
    26161 tgagacaggt caacccttta aaggtgtaac gagacaaggg tatagtgatg attcatgctg
    26221 ggcacgtggt caatcatggg gagtctatgg tattcctttg acttatcgtc acttaaaaga
    26281 cgagtcctgc tttgacttgt ttaagggtgt gaccaattat ttcttgaatc gtctgccaaa
    26341 agatcatgtg tcctattggg atttgatttt taatgatggt agtgctcaat cacgagattc
    26401 ttcagcaaca gctatcgccg tctgtgggat tcatgaaatg ctaaaacatc tcccagaggt
    26461 ggatgctgac aaagatattt ataaacatgc tatgcatgcc atgcttcgtt ccttgatcga
    26521 acattatgca aatgatcaat ttacccctgg tgggacaagt ctcctccacg gtgtgtactc
    26581 atggcattca ggtaaaggag tggatgaagg caatatctgg ggtgactact attacctaga
    26641 agcccttatc cgtttctaca aagactggaa cctatattgg taggaggaga aatatgacaa
    26701 tgccaaatat tattatgacc cgtatcgatg aacggttgat tcatgggcaa ggacaacttt
    26761 gggtaaaata cctaggttgt aatacggtca ttgttgccaa tgacgaagta agcacagaca
    26821 agatgcagca aactctgatg aaaacagttg tgccagactc agttgccatg cgtttcttcc
    26881 ctttgcaaaa ggtgattgat atcattcaca aggctaatcc tgctcaaacg atctttatcg
    26941 ttgtaaagga tgtgaaggac gctttaacct tggtagaagg tggtgtccct atcaaagaaa
    27001 tcaatattgg gaacattcac aatgctactg gtaaagagca agtgacacgc tccatcttcc
    27061 tgggtgaaga ggacaaggcg gccctcaagg aattgagcca aactcatcaa gtaacattta
    27121 atacgaaaac aactccaaca ggaaatgatg gagctgttca agtcaacatt atggactata
    27181 tttaaaagag gagatcgtta tgtcgattaa tgtatttcaa gcgattttaa ttggattatg
    27241 gacagctttc tgttttagtg gaatgctgtt aggaatttac accaatagat gtattgttct
    27301 gtcatttggt gtcggaatta ttctaggtga tttgcctact gctcttgcaa tgggagctat
    27361 tggtgaattg gcttatatgg gattcggtgt tggtgctgga ggtactgttc caccaaaccc
    27421 aatcggacct ggtatctttg gtaccttgat ggctatcact agtgctggta aagtcagtcc
    27481 agaagcggct cttgccctct ctactccgat tgctgtggcg attcaattct tacaaacttt
    27541 cgcctacact gtacgtgctg gtgcgcctga aacggcgatg aagcacttga aaaaccataa
    27601 tttgaagaaa ttcaagttca ctctaaatgc aacaatttgg ttgtttgcct ttattggatt
    27661 taccttgggt tgcttgggtg ccctttcaat ggataccttg ttgaaactcg tagactacat
    27721 tccaccagta ttgcttacag gtttgacagt tgctggtaaa atgctcccag ctatcggttt
    27781 tgcgatgatc ttgtcagtga tggctaagaa agagttgatt ccctttgtct tgttgggata
    27841 tgtttgtgca gcttatctaa acatcccaac aattggtatt gcaattgtag gtactatctt
    27901 tgctttgatt gaattttata acaagccaaa aacagcggat catgtggtag aggaggaagc
    27961 acacgatgac tggatctaac aaattaacaa aacgtgatta tcttaaaacg tctttgcggg
    28021 cattcttttg tcaaaatgga tttaactata gtaactatca agggttggga tatgccaatg
    28081 tgatgtatcc tgctttgaaa aaacactatg gagaggatca ggaaggtttc taccaagcct
    28141 tggaagaaaa ctgtgaattc tataatacca acccacactt cctgcctttt attaccagct
    28201 tgcatcttgt aatgttggaa aatggccgac caacaaaaga aacacgtagc atcaagatgg
    28261 ccttgatggg accattggca ggtattgggg attctctttc tcaattctgt ttagctccat
    28321 tgttctcaac catcgcagct tcgtttgctc aagaaggctt ggttgtcggt ccaatcttgt
    28381 tcttccttgc gatgaatacg attttaacag cgattaaatt gtcaactggt ctgtatggat
    28441 acaaactagg aacaactgtg attgataaac taagcgaaca gatggcaacg atttctcgta
    28501 ttgccaatat tatcggtgta accgtaattg ctggtttggc agcgacatct gttaagatta
    28561 tggtgccgat tacctttgct gcaggggaag ttaaagcaga cgctaaacaa agtatcgtaa
    28621 gtattcaggg aatgcttgat aaggttgctc cagctcttct accagcccta tttacacttt
    28681 tagtttacta cttgatcaaa gaaaagaaat ggacaacata taaactcgtt attttaacag
    28741 ttatcatcgg aattatcgga agctggctta agattatagc ttaatggata caacattgtt
    28801 ttatggaata gtgattgtct tggcagtgag tcccctttta ctgtcaagct ttcattctat
    28861 tcgtcagcaa aagttgcttc gcaaacagat ggagcaacga caagagtatt tagcttcttt
    28921 aacatctggt gatgaagtgt tgttattgtc tggaattcat ggaaaaatca tttctatcaa
    28981 agatgacttg gtctccttgc agattgcaaa aggtgtggtc atctatgtag aaaaggaaag
    29041 tgtaatggga aagacaaaag aactgctttt taagtagttc tttttctggt aatagataat
    29101 gaaagaagaa agaagacaat tttttgaaag aatcgatgga aaccaatgtc gtgattatat
    29161 cttgtcccac tgttcaaaag actatgagaa agtcaagagt tcccttgaac gcttgatgga
    29221 caatcgtttt atgtttgata gtccttggga tatggagcct tgttcaaaaa tccatcaaat
    29281 ccagccgatg gtatgggatc aagtatttga agatgatcca gaatggtctt atatgctcaa
    29341 tcgacaagaa tatctcttgc agtttatgat agggtatctg gtagaaggag ataaggacta
    29401 tattcaaaag tgcaagttct ttctatttga ttggattgag caggtgagag aattttctcc
    29461 tcaatccttg atgactagaa ccttggatac gggtattcgt tcctttactt ggttgaaact
    29521 actcttgctt ctcttgaaat ttgacttgct agaggagaaa gaactagaga aaattttggt
    29581 cagtctagaa aagcagattg actttatgaa aagctactat cgcgccaagt acacccttag
    29641 taactggggg attttacaaa caattccgat gcttgctatc tatcatttct tttcagataa
    29701 gatggaccta gaagaagctt accattttgc ttcagaggag ttgaaacagc aaattgagac
    29761 acagatttta ggagatggaa gccagtttga acagtcgatt ctctatcatg tagaggttta
    29821 taaagccttg ctggatttgt gtctcttgct tccagacttg caagatagtt accaagagtt
    29881 gctggaaaag atggcgacct atattcaaat gatgacaggc ttagatggac gaactttggc
    29941 ttttggtgat agcgattcta cagaaacgac agaaattttg agcctgtctg ctgtggtttt
    30001 gaacaaggaa gaccttctta acggtctgga tgttaaagtt gatttgctta gcctcttgtt
    30061 cctggggcga gaaaaggtca agcgactgca ggaatttgaa aggagagctt ggcagcctaa
    30121 gtccatgatc tttgaagact ctggacatgt ctgtattaag gatgaacatc gttatctatt
    30181 tttcaaaaat ggtccgctag gaagtgccca tagccatagt gacgagaata gtttttgctt
    30241 acagtatcaa ggccaaccta ttttcataga tgctgggcgt tattcttatc gggagatata
    30301 tgaacgttat ctcttaaaga gtgcttggag tcattcgacc tgcattgtag atgggaaagc
    30361 tccggaaaga atcacaggat cctgggaata tgaatactat cctcactccc tgttttgtca
    30421 ccataaagaa agggagggag tgcattatat tgagggggct tattggtcag cagaacctga
    30481 tttgccttat cttcacagaa gaaaaatcct catgttggta gaggatgtct ggctcttggt
    30541 ggatgacatc aggtgtcaag gtcagcatga ggcgttgact cagtttatcc ttgacaagga
    30601 tgtgacctat caagatggga aaatcaatca gttgagacta tggagtgaag ttgattttga
    30661 tttggaagat accatcattt ctcctaaata caatgagctt gaaagaagta gcaaactcac
    30721 caagcgccaa ttctttgaga atcagatgct ggattatacc atcattgcgc atgagagttt
    30781 tgaaatcatc cgtcattctg tctaccagac agatgatcgt gaagtggaaa atgctctggc
    30841 ttttgaagtg aaaaatgacg aaacagacaa gctgattctg ttattaagcg aggatattgg
    30901 tgtaggtgaa aaattgtgcc tcgttgacgg aacaaaaatg cgtggaaaat gtctagtata
    30961 tgataaaata aatgagagaa tgattcgctt gcagtgctag aaataggcat tttgaatagt
    31021 gaacatgtta taataagtat tagtaggagg tgttttagat tggagaagaa actgaccata
    31081 aaagacattg cggaaatggc tcagacctcg aaaacaaccg tgtcatttta cctaaacggg
    31141 aaatatgaaa aaatgtccca agagacacgt gaaaagattg aaaaagttat tcatgaaaca
    31201 aattacaaac cgagcattgt tgcgcgtagc ttaaactcca aacgaacaaa attaatcggt
    31261 gttttgatcg gtgatattac caacagtttc tcaaaccaaa ttgttaaggg aattgaggat
    31321 atcgccagcc agaatggcta ccaggtaatg ataggaaata gtaattacag ccaagagagt
    31381 gaggaccggt atattgaaag catgcttctc ttgggagtag acggctttat tattcagccg
    31441 acctctaatt tccgaaaata ttctcgtatc atcgatgaga aaaagaagaa aatggtcttt
    31501 tttgatagtc agctctatga acaccggact agctgggtta aaaccaataa ctatgatgcc
    31561 gtttatgaca tgacccagtc ctgtatcgaa aaaggttatg aacattttct cttgattaca
    31621 gcggatacga gtcgtttgag tactcggatt gagcgggcaa gtggttttgt ggatgcttta
    31681 acagatgcta atatgcgtca cgccagtcta accattgaag ataagcatac gaatttggaa
    31741 caaattaagg aatttttaca aaaagaaatc gatcccgatg aaaaaactct ggtatttatc
    31801 cctaactgtt gggccctacc tctagtcttt accgttatca aaaagttgaa ttataacttg
    31861 ccacaagttg ggttgattgg ttttgacaat acggagtgga cttgcttttc ttctccaagt
    31921 gtttcgacgc tggttcagcc ctcctttgag gaaggacaac aggctacaaa gattttgatt
    31981 gaccagattg aaggccgcaa tcaagaagaa aggcaacaag tcttggattg tagtgtgaat
    32041 tggaaagagt cgactttcta aaatgaagga aaatgacttg caatctctgt taagaaataa
    32101 aataatccca cctagaacaa gctaggtggg attatttgcc tatgaaatga gaaattatgg
    32161 gagcaagctc ctaaatcaac tgtttttgat ctacttcttt aactacttga taaaagttat
    32221 agaagtaggc caaacttgaa atgatggtta cgactaggaa tattgaaaat ttccattgga
    32281 caggggtggt taaaagttgt ggaaaggata tgaggagaaa gaagagggct gcgttgagga
    32341 caggtatccg ttttgattgt attttctcaa gtcctttatt gagcgcagga agaaagagga
    32401 gtaggagtag taaaactgta tgagaaacag ctcctgaaat aagggcgaag aaaaggaaaa
    32461 tactgataaa aacatgaatg atcagtagtc tagctagtga tttcataagg cacctcctaa
    32521 tcctggtctt ttttagctct tgcaatacga agtgagtcga caatatgtat catcactccg
    32581 aaaaagaaag ctcccagtat agttttaaaa atatgttttg tatttagaag agaactgata
    32641 aaatttggat tttcacttgt tagggtatca atgagtggaa ttataaaaaa tatcactgtt
    32701 ccataaatcg aacctgcttt cagaccagga taacgtaact gtttcttttc ttttttcatg
    32761 agtttcctcc taatcctcat cttgattttt cttagttttt gcaatgcgac gggagatgag
    32821 gaactgtatg ctcgctccga agaaaataga accgagaaca cttgatacac catttcttat
    32881 agtgagaaga gaatgaaaat agtcctgacc ttcatctatg agtatactga gaagaggagt
    32941 tataaaaaac atccatagac caaagaacaa acctgctttc agacctgggt agtgtagttg
    33001 cttgctttct ttctcattca gcatatctgg ttcaatgact gtgatgcctg tttttttcat
    33061 ttggtaggtg acatagccag cagcgatgag ggcaatcact aaaatcagag gaggatagat
    33121 tagagccact tcttgagggt atttataggc cagaaggagt ggaataagat ttccgaaaat
    33181 catcagataa aagaggatga taaaaacttg gttcccaata ctatcggcct cacgccgttt
    33241 gtattcgtca aggggaccag aaataccgta tgtgcgtttg atcagttttt cagtgaaggt
    33301 ttcttttttc ataagtttgc tcctttttta aaaatcttcc tcccaaaaga gactgttgag
    33361 gtcagtttgg aggctgcggg cgagattgag acagagttcc aaggttggat tgtacttgtc
    33421 gttttcaatc atattgatag tctgtctcga gacaccgata tccttggcga gttcgagctg
    33481 ggaaataccc aattccttgc gaaattcttt cacacgattc atctgttctc ctttctgatt
    33541 tatgtcgtat atatttgact atattatagt cttttaaaca taaagtgtca agtatttttg
    33601 acatattttt tgaagaaata gtagtctcct tgtcctattt gtctgacaag tgcaagctgg
    33661 tcggatttgt ggtaaaatag ataagatatg acaaaagaat ttcatcatgt aacggtctta
    33721 ctccacgaaa cgattgatat gcttgacgta aagcctgacg gtatctacgt tgatgcgact
    33781 ttgggcggag caggacatag cgagtattta ttaagtaaat taagtgaaaa aggccatctc
    33841 tatgcctttg accaggatca gaatgccatt gataatgcgc aaaaacgctt ggcaccttac
    33901 attgagaagg gaatggtgac ctttatcaag gataacttcc gtcatttaca ggcacgtttg
    33961 cgcgaagctg gtgttcagga aattgatgga atttgttatg acttgggagt gtctagtcct
    34021 caattagacc agcgtgagcg tggtttttct tataaaaagg atgcgccact ggacatgcgg
    34081 atgaatcagg atgctagtct gacagcgtat gaagtggtga acaattatga ctatcatgac
    34141 ttggttcgta ttttcttcaa gtatggagag gacaaattct ctaaacagat tgcgcgtaag
    34201 attgagcaag cgcgtgaagt gaagccgatt gagacaacga ctgagttagc agagattatc
    34261 aagttggtca aacctgccaa ggaactcaag aagaaggggc atcctgctaa gcagattttc
    34321 caggctattc gaattgaagt caatgatgaa ctgggggcgg cagatgagtc catccaccag
    34381 gctatggata tgttggctct ggatggtaga atttcagtga ttacctttca ttccttagaa
    34441 gaccgcttga ccaagcaatt gttcaaggaa gcttcaacag ttgaagttcc aaaaggcttg
    34501 cctttcatcc cagatgatct caagcccaag atggaattgg tgtcccgtaa gccaatcttg
    34561 ccaagtgcgg aagagttaga agccaataac cgctcgcact cagccaagtt gcgcgtggcc
    34621 agaaaaattc acaagtaaga gggaaaaaga tggcagaaaa aatggaaaaa acaggtcaaa
    34681 tactacagat gcaacttaaa cggttttcgc gtgtggaaaa agctttttac ttttccattg
    34741 ctgtaaccac tcttattgta gccattagta ttatttttat gcagaccaag ctcttgcaag
    34801 tgcagaatga tttgacaaaa atcaatgcgc agatagagga aaagaaaacc gaattggacg
    34861 atgccaagca agaggtcaat gaactattac gtgcagaacg tttgaaagaa attgccaatt
    34921 cacacgattt gcaattaaac aatgaaaata ttagaatagc ggagtaagat atgaagtgga
    34981 caaaaagagt aatccgttat gcgaccaaaa atcggaaatc gccggctgaa aacagacgca
    35041 gagttggaaa aagtctgagt ttattatctg tctttgtttt tgccattttt ttagtcaatt
    35101 ttgcggtcat tattgggaca ggcactcgct ttggaacaga tttagcgaag gaagctaaga
    35161 aggttcatca aaccacccgt acagttcctg ccaaacgtgg gactatttat gaccgaaatg
    35221 gagtcccgat tgctgaggat gcaacctcct ataatgtcta tgcggtcatt gatgagaact
    35281 ataagtcagc aacgggtaag attctttacg tagaaaaaac acaatttaac aaggttgcag
    35341 aggtctttca taagtatctg gacatggaag aatcctatgt aagagagcaa ctctcgcaac
    35401 ctaatctcaa gcaagtttcc tttggagcaa agggaaatgg gattacctat gccaatatga
    35461 tgtctatcaa aaaagaattg gaagctgcag aggtcaaggg gattgatttt acaaccagtc
    35521 ccaatcgtag ttacccaaac ggacaatttg cttctagttt tatcggccta gctcagctcc
    35581 atgaaaatga agatggaagc aagagcttgc tgggaacctc tggaatggag agttccttga
    35641 acagtattct tgcagggaca gacggcatta ttacctatga aaaggatcgt ctgggtaata
    35701 ttgtacccgg aacagaacaa gtttcccaac gaacgatgga cggtaaggat gtttatacaa
    35761 ccatttccag ccctctccag tcctttatgg aaacccagat ggatgctttt caagagaagg
    35821 taaaaggaaa gtacatgaca gcgactttgg tcagtgctaa aacaggggaa attctggcaa
    35881 caacgcaacg accgaccttt gatgcagata caaaagaagg cattacagag gactttgttt
    35941 ggcgtgatat cctttaccaa agtaactatg agccaggttc cactatgaaa gtgatgatgt
    36001 tggctgctgc tattgataat aatacctttc caggaggaga agtctttaat agtagtgagt
    36061 taaaaattgc agatgccacg attcgagatt gggacgttaa tgaaggattg actggtggca
    36121 gaatgatgac tttttctcaa ggttttgcac actcaagtaa cgttgggatg accctccttg
    36181 agcaaaagat gggagatgct acctggcttg attatcttaa tcgttttaaa tttggagttc
    36241 cgacccgttt cggtttgacg gatgagtatg ctggtcagct tcctgcggat aatattgtca
    36301 acattgcgca aagttcattt ggacaaggga tttcagtgac ccagacacaa atgattcgtg
    36361 cctttacagc tattgctaat gacggtgtca tgctggagcc taaatttatt agtgccattt
    36421 atgatccaaa tgatcaaact gctcggaaat ctcaaaaaga aattgtggga aatcctgttt
    36481 ctaaagatgc agctagtcta actcggacta acatggtttt ggtagggacg gatccggttt
    36541 atggaaccat gtataaccac agcacaggca agccaactgt aactgttcct gggcaaaatg
    36601 tagccctcaa gtctggtacg gctcagattg ctgacgagaa aaatggtggt tatctagtcg
    36661 ggttaaccga ctatattttc tcggctgtat cgatgagtcc ggctgaaaat cctgatttta
    36721 tcttgtatgt gacggtccaa caacctgaac attattcagg tattcagttg ggagaatttg
    36781 ccaatcctat cttggagcgg gcttcagcta tgaaagactc tctcaatctt caaacaacag
    36841 ctaaagcttt ggagcaagta agtcaacaaa gtccttatcc tatgcctagt gtcaaggata
    36901 tttcacctgg tgatttagca gaagaattgc gtcgcaatct tgtacaaccc atcgttgtgg
    36961 gaacaggaac gaagattaaa aacagttctg ctgaagaagg gaagaatctt gccccgaacc
    37021 agcaagtcct tatcttatct gataaagcag aggaagttcc agatatgtat ggttggacaa
    37081 aagcgactgc tgagaccttt gctaagtggc tcaatataga acttgaattt caaggttcgg
    37141 gctctactgt gcagaagcaa gatgttcgtg ctaacacagc tatcaaggac attaaaaaaa
    37201 ttacattaac tttaggagac taatatgttt atttccatca gtgctggaat tgtgacattt
    37261 ttactaactt tagtaggaat tccggccttt atccaatttt atagaaaggc gcaaattaca
    37321 ggccagcaga tgcatgaaga tgtcaaacag catcaggcaa aagctgggac tcctacaatg
    37381 ggaggtttgg ttttcttgat tacttctgtt ttggttgctt tctttttcgc cctatttagt
    37441 agccaattca gcaataatgt gggaatgatt ttgttcatat tagtcttgta tggcttggtc
    37501 ggatttttag atgactttct caaggtcttt cgtaaaatca atgaggggct taatcctaag
    37561 caaaaattag cgcttcagct tctaggtgga attatcttct atcttttcta tgagcgtggg
    37621 ggcgatatcc tgtctgtctt tggttatcca gttcatttgg gatttttcta tattttcttc
    37681 gctcttttct ggctagtcgg tttttcaaac gcagtaaact tgacagacgg tgttgacggt
    37741 ttagctagta tttccgttgt gattagtttg tctgcctatg gagttattgc ctatgtgcaa
    37801 ggtcagatgg atattcttct agtgattctt gccatgattg gtggtttgct cggtttcttc
    37861 atctttaacc ataagcctgc caaggtcttt atgggtgatg tgggaagttt ggccctaggt
    37921 gggatgctgg cagctatctc tatggctctc caccaagaat ggactctctt gattatcgga
    37981 attgtatatg tttttgaaac aacttctgtt atgatgcaag tcagttattt caaactgaca
    38041 ggtggtaaac gtattttccg tatgacgcct gtacatcacc attttgagct tgggggattg
    38101 tctggtaaag gaaatccttg gagcgagtgg aaggttgact tcttcttttg gggagtggga
    38161 cttctagcaa gtcttctgac cctagcaatt ttgtatttaa tgtaagaatg gcatcctgat
    38221 gtttcagggt gtttttgtat ttaaatacac aatgaaaatc aaagaacaaa ctagaaagct
    38281 aactttaggc tgctcaaaat atagtagatt gaaactagaa tagtacacat ctacttctaa
    38341 aatattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt atttcatttt
    38401 aatatatagt tttgagatgg ttgatagaac tgacgaagtc agctcaaaac atggttttga
    38461 ggttgtagat aaggagaagc tgatgtggtt tgaagaggtt ttcgaagagt gtaaaaaaat
    38521 attggtcagt taaagccaaa actcttgacc ttttctgacc aatgtagtat attatgaacg
    38581 taaggtaaat gaaagcctta ctagaacact tatttaaagt aaaatagaaa gaggtgggtc
    38641 tatgtttcat ctagaaatct tcagaagtaa agatggtcta ctcctgcttg aaaaagaaaa
    38701 accagaaata gtacgtaggg tagcggttta actagtctaa attttttaaa acaatggtca
    38761 aagatagtca atatcagtaa tcataactaa gtaaacaaaa agaggtaaag aatatgaata
    38821 acaactttaa taattttaat aacatggatg atttatttaa ccaattgatg ggtggtatgc
    38881 gaggatatag ttctgaaaat cgccgttact taattaatgg acgcgaagtc acatctgagg
    38941 aatttgctca ctatcgtgcg actggtcaat taccaggaaa tgcagaaact gatgtgcaaa
    39001 tgccacaaca ggcatcaggt atgaaacaag acggtgtcct tgcaaaacta ggtcgaaact
    39061 tgacagcaga agcgcgtgag ggcaagttgg atcctgttat cggacgaaac aaggaaattc
    39121 aagaaacatc tgaaatcctc tcacgccgca ccaagaacaa tcctgttttg gtcggagatg
    39181 caggtgttgg taagacagca gttgtcgaag gtctagcgca agccattgtg aacggagatg
    39241 ttcctgctgc tatcaagaac aaggaaatta tttctattga tatctcaggt cttgaggctg
    39301 gtactcaata ccgtggtagc tttgaagaaa atgtccaaaa cttagtcaat gaagtgaaag
    39361 aagcagggaa tattatcctc ttctttgatg aaattcacca aattcttggt gctggtagca
    39421 ctggtggaga cagtggttct aaaggacttg cggatattct caagccagct ctctctcgtg
    39481 gagaattgac agtgattggg gcaacaactc aagacgaata ccgtaacacc atcttgaaga
    39541 atgctgctct tgctcgtcgt ttcaacgaag tgaaggtcaa tgctccttca gcagagaata
    39601 cttttaaaat tcttcaaggc attcgtgacc tctatcaaca acatcacaat gtcatcttgc
    39661 cagacgaagt cttgaaagca gcggtggatt attctgttca atacattcct caacgtagct
    39721 tgccagataa ggctattgac cttgtcgatg taacagctgc tcacttggcg gctcaacatc
    39781 cagtaacaga tgtgcatgct gttgaacgag aaatcgaaac ggaaaaagac aagcaagaaa
    39841 aagcagttga agcagaagat tttgaagcag ctctaaacta taaaacacgc attgcagaat
    39901 tggaaaagaa aatcgaaaac cacacagaag atatgaaagt gactgcaagt gtcaacgatg
    39961 tggctgaatc tgtggaacga atgacaggta tcccagtatc gcaaatggga gcttcagata
    40021 tcgaacgttt gaaagatatg gctcatcgct tgcaagacaa ggtgattggt caagataagg
    40081 ccgtagaagc tgtagctcgt gctatccgtc gtaaccgtgc tggttttgat gaaggaaatc
    40141 gcccaatcgg cagcttcctc tttgtagggt ctactggggt tggtaagacg gagcttgcta
    40201 agcaattggc actcgatatg tttggaaccc aggatgcgat tatccgtttg gatatgtctg
    40261 aatacagtga ccgcacagct gtttctaagc taattggtac aacagcaggc tatgtgggtt
    40321 atgatgacaa tagcaatacc ttaacagaac gtgttcgtcg caatccatac tctatcattc
    40381 tcttggatga aattgaaaag gctgatcctc aagttattac ccttctcctc caagttctag
    40441 atgatggtcg tttgacagat ggtcaaggaa atacagtaaa cttcaagaac actgtcatta
    40501 ttgcgacctc aaatgctgga tttggctatg aagccaactt gacagaagat gcggataaac
    40561 cagaattgat ggaccgtttg aaacccttct tccgtccaga attcctcaac cgctttaatg
    40621 cagtcatcga gttctcacaa ttgactaagg aagacctttc taagattgta gatttgatgt
    40681 tggctgaagt taaccaaacc ttggctaaga aagacattga cttggtagtc agtcaagcgg
    40741 ctaaagatta tatcacagaa gaaggttacg acgaagtcat gggggttcgt cctctccgtc
    40801 gcgtggttga acaagaaatt cgtgataagg tgacagactt ccacttggat catttagatg
    40861 ctaaacatct ggaagcagat atgaaagatg gcgttttggt tattcgtgag aaagcctaag
    40921 acagaatttt gaggataaaa aagaaggagc cagctgaaaa aactggttcc ttttgaggta
    40981 cgacgggcat gtcgtatagt agaagtg
//
LOCUS       DAVBCR010000010        37639 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCR010000010 DAVBCR010000000
VERSION     DAVBCR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.010"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VQ508_000473"
     CDS             51..299
                     /locus_tag="VQ508_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489526.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            430..1602
                     /locus_tag="VQ508_000474"
     CDS             430..1602
                     /locus_tag="VQ508_000474"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2489527.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VQ508_000475"
     CDS             1602..2876
                     /locus_tag="VQ508_000475"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2489528.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VQ508_000476"
     CDS             complement(2912..3877)
                     /locus_tag="VQ508_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2489529.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VQ508_000477"
     CDS             complement(3858..5312)
                     /locus_tag="VQ508_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2489530.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5689..7572
                     /locus_tag="VQ508_000478"
     CDS             5689..7572
                     /locus_tag="VQ508_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2489531.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VQ508_000479"
     CDS             7721..8608
                     /locus_tag="VQ508_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2489532.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8928..9545)
                     /locus_tag="VQ508_000480"
     CDS             complement(8928..9545)
                     /locus_tag="VQ508_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2489533.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10148)
                     /locus_tag="VQ508_000481"
                     /pseudo
     CDS             complement(9960..10148)
                     /locus_tag="VQ508_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10658..10738)
                     /locus_tag="VQ508_000482"
                     /pseudo
     CDS             complement(10658..10738)
                     /locus_tag="VQ508_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10854..11747
                     /locus_tag="VQ508_000483"
     CDS             10854..11747
                     /locus_tag="VQ508_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489534.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11744..12943
                     /locus_tag="VQ508_000484"
     CDS             11744..12943
                     /locus_tag="VQ508_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489535.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13296..14063)
                     /locus_tag="VQ508_000485"
     CDS             complement(13296..14063)
                     /locus_tag="VQ508_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489536.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYTGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14080..14775)
                     /locus_tag="VQ508_000486"
     CDS             complement(14080..14775)
                     /locus_tag="VQ508_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489537.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14780..15145)
                     /locus_tag="VQ508_000487"
     CDS             complement(14780..15145)
                     /locus_tag="VQ508_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2489538.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            15285..15758
                     /gene="nrdR"
                     /locus_tag="VQ508_000488"
     CDS             15285..15758
                     /gene="nrdR"
                     /locus_tag="VQ508_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2489539.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15759..16928
                     /locus_tag="VQ508_000489"
     CDS             15759..16928
                     /locus_tag="VQ508_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2489540.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            16929..17825
                     /gene="dnaI"
                     /locus_tag="VQ508_000490"
     CDS             16929..17825
                     /gene="dnaI"
                     /locus_tag="VQ508_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2489541.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17822..18535
                     /locus_tag="VQ508_000491"
     CDS             17822..18535
                     /locus_tag="VQ508_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2489542.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18549..19859
                     /gene="der"
                     /locus_tag="VQ508_000492"
     CDS             18549..19859
                     /gene="der"
                     /locus_tag="VQ508_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2489543.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            19950..20285
                     /locus_tag="VQ508_000493"
     CDS             19950..20285
                     /locus_tag="VQ508_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489544.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20425..20760
                     /locus_tag="VQ508_000494"
     CDS             20425..20760
                     /locus_tag="VQ508_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489545.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            20945..21736
                     /locus_tag="VQ508_000495"
     CDS             20945..21736
                     /locus_tag="VQ508_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489546.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21729..22370
                     /locus_tag="VQ508_000496"
     CDS             21729..22370
                     /locus_tag="VQ508_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489547.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22374..23024
                     /locus_tag="VQ508_000497"
     CDS             22374..23024
                     /locus_tag="VQ508_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2489548.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23035..23322
                     /locus_tag="VQ508_000498"
     CDS             23035..23322
                     /locus_tag="VQ508_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489549.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23468..25981
                     /gene="secA"
                     /locus_tag="VQ508_000499"
     CDS             23468..25981
                     /gene="secA"
                     /locus_tag="VQ508_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2489550.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26062..27093
                     /locus_tag="VQ508_000500"
     CDS             26062..27093
                     /locus_tag="VQ508_000500"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2489551.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27095..28126
                     /locus_tag="VQ508_000501"
     CDS             27095..28126
                     /locus_tag="VQ508_000501"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2489552.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28165..28533
                     /locus_tag="VQ508_000502"
     CDS             28165..28533
                     /locus_tag="VQ508_000502"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2489553.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28523..29626
                     /locus_tag="VQ508_000503"
     CDS             28523..29626
                     /locus_tag="VQ508_000503"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2489554.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29645..31660
                     /gene="recG"
                     /locus_tag="VQ508_000504"
     CDS             29645..31660
                     /gene="recG"
                     /locus_tag="VQ508_000504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2489555.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            31976..32956
                     /locus_tag="VQ508_000505"
     CDS             31976..32956
                     /locus_tag="VQ508_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2489556.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33761..33988
                     /locus_tag="VQ508_000506"
     CDS             33761..33988
                     /locus_tag="VQ508_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489557.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34276..37173
                     /gene="nanA"
                     /locus_tag="VQ508_000507"
     CDS             34276..37173
                     /gene="nanA"
                     /locus_tag="VQ508_000507"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2489558.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT        11273 a         7011 c         8379 g        10976 t
ORIGIN      
        1 tactttcctt attgtctgta aatctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgataagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga tttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca tcgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gctatggggc gacagctgcc
      781 ctccattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatatgg tattgaaact ccgggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtctatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatgcaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagtgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgccagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagtttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 taggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatggcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgct atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctt tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagccctca
     2701 aagctttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtagagcgc ctcatctcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcaactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgtagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagtctggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 cgacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtccacc tgattggctt
     3541 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attagggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggatgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4741 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgact tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatctggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa atttttacga aaatgtgaaa
     5461 gataatgaaa taaaagactt tttaaccaag gtttcaaact tatttttcaa ggaattcctg
     5521 atgaaacagt caaaaacaat caatctaaaa cttgcccact aagtggatag aatatttttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg cttaccaaca
     5941 tcatctaagg atgacatgaa agcagaagtt gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt tcggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cacttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcgatg
     6301 aacttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttgtc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagt caaggcgtgg tctatgcact agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcacgttgg tattgacact gtatctatga acggtgacgg ttttgaagca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacagctgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagtttcga agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatgt ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctaaacgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaaatggt tgctcgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggcgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt acaagtttgg aagctcgtac aggtgtacgt ggggaaacta ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaagttt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag cctagtgtct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctatcacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcataat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcagaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatagtcat gtagcgcttg aaactcatct tggtcagggc ggccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcact agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaaacag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagtattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttatatgta ttctgaagct agaaagacta aaatataaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgagt
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgataaatgc gacttccttt
    10081 gtagttgttt tcttagttcc ctgaggtaac catctatgaa tgagcctgtt gtgattttca
    10141 ttagatccta tagtgatgtg cataatagat atagcagatt gaaactagaa tagtatacat
    10201 ctacttctaa aacattgtta gaaatcaatt tgactgtcct gatcaatttg tcctgttttt
    10261 atttcatttt actatataat ccttagaaaa catatcggac aagcggttga attccgttcc
    10321 gtccattagt agataaagga ttactccttc ttgaaatgac agatgaactt gtttcttata
    10381 aaaatgttgt ctgaaactat tagccttttt ccagaagttt tagaggaaga aaactttacg
    10441 aggaaaaagg agttactcaa ataacaattc aggattaaaa atagacagtt gaggagcgga
    10501 aggtataaat taagtttgct actgtataat ggatttatca ctaatactct tcgaaaatct
    10561 cttcaaacca cgtcagcttc gccttgccgt agatatagtt actgacttcg tcagttctat
    10621 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tttatctgca acctcaaagc
    10681 tgtactttga gcagcctacg gctagcttcc tagtttgctc tttgcttttc attgagtata
    10741 aaagtgctcc aggccatatt ttacacagca atagtcttct aaaaatggac aaaaacattg
    10801 taaaggctat caaaaaggag tataatgagt gcaagaaatt tcggaggtga aagatgctag
    10861 aagtaagaag tctagagaaa agttttggct ccaagcaagt tttgtttggt attgactttc
    10921 aagcgcgacc aggtcgtatt ttgggactag tcggaaaaaa tggtgctgga aaaacaacga
    10981 ttttccatag tattttgaaa ttcctagaat atcagggaga aatcggtctg gatggtcagg
    11041 atattcgtca ggagacctat gctcggattg gttatctgcc tgaagaacgc agtctcatgc
    11101 ctaaattgac agttcttgaa caagttcgct acttggcgac tctaaaaggc atggatgcca
    11161 aagaagtcaa agaaaaactc cctcaatgga tgaagaggtt ggaagtgaaa ggaaagctga
    11221 cagataaaat caagagtctg tcaaaaggaa atcagcagaa gattcagctc attattactc
    11281 tgattcatga accagacctg attatcttgg atgagccttt tagtggattg gacccagtta
    11341 atacagaatt gctcaaacaa gtcatttttc aggaaaaaga gcgcggagca accattatct
    11401 tttctgacca tgtcatgacc aatgttgagg aactttgtga cgatattctg atgatccgag
    11461 atggccgtgt ggtcttgcac ggaccagttc aggatgtccg caatcaatac gggaaaacgc
    11521 gtctctttgt ttcaagtgaa cgaagcaagg aagaattgga aaatcttcct catgtcaaac
    11581 aggtgagctt gaccaaacaa ggcagttgga aattgatttt ggaggatgag agcgctggaa
    11641 gggaactctt cccaatcttg actcaaggtc aatatatcgc aacatttgac caacaagcgc
    11701 caactatcga tgaaatcttt aaactagaat caggagtgga agtatgagaa atatgtgggt
    11761 tgtaatcaag gaaacctatc ttcgacatgt cgagtcatgg agtttcttct ttatggtgat
    11821 ttcgccgttc ctctttttag gaatctctgt aggaattggg catctccaag gttcttctat
    11881 ggctaaaaat aataaagtgg cagtagtgac aacagtgcca tctgtagcag aaggactgaa
    11941 gaatgtaaat ggtgttaact tcgactataa agacgaagca agtgccaaag aagcaattaa
    12001 agaagaaaaa ttaaaaggtt atttgaccat tgatcaagaa gatagtgttc taaaggcagt
    12061 ttatcatggc gaaacatcgc ttgaaaatgg aattaaattt gaggttacag gtacactcaa
    12121 tgaactgcaa aatcagctta atcgttcaac tgcttccttg tctcaagagc aggaaaaacg
    12181 cttagagcag acaattcaat tcacagaaaa gattgatgaa gccaaggaaa ataaaaagtt
    12241 tattcaaaca attgcagcag gtgccttagg attctttctt tatatgattc tgattaccta
    12301 tgcgggtgta acagctcagg aagttgccag tgaaaaaggc accaaaatta tggaagtcgt
    12361 tttttctagc ataagggcaa gtcactattt ctatgcgcgg atgatggctc tgtttctagt
    12421 gattttaacg catattggga tctatgttgt aggtggtctg gctgccgttt tgctctttaa
    12481 agatttgcca ttcttggctc agtctggtat tttggatcac ttgggagatg ctatctcact
    12541 gaataccttg ctctttattt tgatcagtct tttcatgtac gtagtcttgg cagcattcct
    12601 aggatctatg gtttctcgtc ctgaggactc agggaaagcc ttgtcgcctt tgatgatttt
    12661 gattatgggt ggtttttttg gagtgacagc tctaggtgca gctggtgaca atctcctctt
    12721 gaagattggt tcttatattc cctttatttc gaccttcttt atgccgtttc gaacgattaa
    12781 tggctatgcg gggggagcag aagcatggat ttcacttgct attacagtga tttttgcggt
    12841 ggtagcaaca ggatttatcg gacgcatgta tgctagtctc gttcttcaaa cggatgattt
    12901 agggatttgg aaaaccttta aacgtgcctt atcttataaa tagaagagcc ttgcgaaagc
    12961 gaggttttta aaggtgaaaa gggtggaatc acgaacctaa tgatgtacac attatgtaaa
    13021 caggttgctt gaaaactcaa ttgtatagtg aactgaatct ggaataggat aatattactt
    13081 ctaaaacatt cttataaatt gatttgaatt ccaaaatccc atcgttccgt tcttatttca
    13141 ttttgcgata tagtgaaagg aaataagtca gctcacaaaa aacgcataat atcaaggatt
    13201 tttaacactt gatactatgc gttttctaat tttaaagact ttttgcctgg tctcttttgt
    13261 tctatgagta tgttacatat ctaggtaaaa attatttaaa gcaaattaac cttatttctc
    13321 aagatgtagt aggttccgag ataaaagata gctatgaata tgagttcttt aacaatggct
    13381 atacctgctc ccgtatagaa atggtcattg agtcctacca gtgaattgac atagaaatta
    13441 gaactaagat tgaagaaaag ttcaataaat ccaatgacga tttggatacc aatgtaggct
    13501 gcaacagcga gtgctgtacg gtattcattg aaaagctgtc caatggaaat agccaggtag
    13561 atgcagagga ttcctgaaat ggtatttagt aggaaggata tacctgtaag aaagatctga
    13621 gggagatgtg tttctacaaa tgtaatcaca taagaaagag gaatccattc tggagctgtt
    13681 aaagccagaa taataacagc acttagagcc aatacagcgg tgctaatcaa tgaccagata
    13741 aaggcaccga ttagtttggc tgtgatgata tggtgttcag aaactggcaa ggtcaaagtc
    13801 agatagcctt gtcggtcgta gacactacct ttgaagcgtt taataatcaa gaaaatggtt
    13861 gaaatcgcaa gtgtaagcat caagccacca aagactgtag ctagaaaaag tagcatagta
    13921 gcctgacttt cttggtaagg tagattttta aagccttgtg tctgtattcc gatgagggca
    13981 gaaaggacta gcacggctgc gtagagggct aaataccact tgttaacatt tttaaattcg
    14041 tagtgaacta aattccaaaa cataaataat ctcctttgct taggccttaa attcctgacg
    14101 gaagagttgg tcaatggatt cacctgactc gtagcgaata tcatctacat ttccttgacg
    14161 gacgactttt ccgtctttta ggaagacaat ttcatccaag attggctcga tatcagaaat
    14221 caagtgggta gaaatcaaaa cggtagaagt tggtgagtag ttgttgataa tggtattgag
    14281 gatataagca cgggctgctg gatccacccc accaatgggt tcatccaaaa catagagacg
    14341 agcatcacgg ctcataacca aaatcagttg aaccttttct ttgtttcctt ttgatagttt
    14401 cttgagacga ctattttcat caatgcccag gtctgcaagt agatgatgag cgcgttcaag
    14461 attgaaatct ttatagaagg tcttgaagta ggttagggct tctttgacct tcatttgctc
    14521 attgagatag gtcgtatcag gcaaataagc tacaacggcc ttggttgctg ggcttgggtc
    14581 catgtcgttg atgaggacac gtccttgatc tggttgtaag aggccattaa ttagtttaat
    14641 cagggttgtt tttcctgagc cgtttggccc aagaaggccg acaatttttc cagctggaat
    14701 gtcaagagaa acattttcaa gggctggtgt tgctccatat gatttggata cattttcaaa
    14761 tactagtaat gacataggct taaactcctt taatataatc actgactacg cctggtagtt
    14821 cttctttttc atagccaaaa tggatcatgg aggaaacgaa gtgttccaat tcttcttctg
    14881 ataattgttt gcgtgactga gcgattagct ccttatcctt agtcacaaat cgtccagttg
    14941 ttcgcttgct gtagacaaat ccttctcgtt caaggtctga taaggctctt tggatggtat
    15001 tgggattgac accagcctcg ctagctagct ccctcacggt tggaagttgt tgattgggtt
    15061 ccagtgtatg ggaaacaatc tgaagcttga ttttctccat aatctgtaaa tagatgggtt
    15121 ttttgttgtc aaatgtccag gacatcttag tctcctttct cttatccttt tgtcttaact
    15181 aaataataca acaaaacaaa aaacttgtca agcaaaaaga agaattgttt tgggaatcgc
    15241 ttaaaaaatg gtataatgaa aagaaaacga taaggaggga aaggatgcgt tgtccaaaat
    15301 gtggggctac caagtcaagt gttatcgata gtcgccaagc agaagaaggg aacaccattc
    15361 gtagaagacg tgagtgcgac gaatgccaac accgttttac aacctacgaa cgagtagaag
    15421 aaagaacctt agtggttgtt aaaaaagatg gtacacggga acaattctcc agagataaaa
    15481 tctttaatgg gattatccgc tcagcccaga aacgtcctgt gtcaagtgat gaaatcaaca
    15541 tggtagtcaa tcgtatcgaa cagaaactcc gtggtcgaaa tgaaaatgaa attcaaagtg
    15601 aggacattgg ttcactcgtc atggaggagt tggctgaatt ggacgagatt acctatgtac
    15661 gttttgctag tgtctatcgt agttttaagg atgtcagtga gttagagagc ttgctccaac
    15721 aaatcaccca gtcctctaaa aagaaaaagg aaagataaat gaagccaatt gaccgttttt
    15781 cttatctaaa gaataatcgg gtgtcgcaag atacctcatc tctggtacag tgctacctcc
    15841 cgattatcgg tcaggaggca ctgagccttt atctttatac aatcagtttt tgggataatg
    15901 gtagaaagga atatcttttt tcaagtatcc tcaatcatct caactttggg atggatagac
    15961 tgataaaatc attgaaaatc ttatctgctt ttaatctctt gaccctctat caaaaggggg
    16021 atgtttatca gctagccctc catgctcctc tatctagtca agacttcttg gagcatcctg
    16081 tttatcgcag actcttagag aaaaagattg gcgactctgc tgtggaggat ttgaaagttg
    16141 aaagtgctga tggtgaagaa atacctgtct cactcaatca agtctttcca gaattggcag
    16201 aactaggcag tcaagaagac cttggtctca agaagaaagt ggccaatgat tttgacttgg
    16261 aacattttcg ccagctgatg gctcgagatg gacttcgttt tgcggatgag cagtccgatg
    16321 tcttaaacct ctttgctatt gccgaggaga agaaatggac ttggtttgaa acctatcaat
    16381 tggccaagtc aacagctgtt tctcaggtta tttcaaccaa acgcatgcgt gaaaaaattg
    16441 ctcaaaaacc agtttcctct gactttaatc ctaaggaagc aaccattatc aaggaagcta
    16501 aaagtaagac tgccctgcag ttcttagcag aaatcaagca aacacgcaag gggaccatta
    16561 cccaaacaga aagagaactt ttgcaacaga tggctggctt gggcttgctg gacgaagtca
    16621 tcaatatcat tctcttgttg acctttaata aggtggattc ggcaaacatc aatgagaaat
    16681 atgccatgaa ggtggctaat gactatgcct atcaaaagat acattcagca gaagaggccg
    16741 ttttacgaat tcgtgagcga gggcagaaaa caaaaacaca aaaacagaat cagactgccc
    16801 cagcaaaaac caatattcct aaatggagta acccagatta taagaatgaa accagcgagg
    16861 aaactcgtct ggaactagaa cgtaagaaac aagaactatt agctcgatta gaaaaaggag
    16921 gagattagat ggaaagtgtc ggagacgtac tcaaacgtca acccagccgt ttttattatc
    16981 aagatttggt ccagaaaatc atgaaggacc ctgatgttgc ggcttttatc cagcaagaat
    17041 ccttgactcc agaggaatta aatcgcagta tctccaagtt taatcagtac atcaccgagc
    17101 gtgacaagtt tctccgtggg gatacggatt atattgccaa aggctacaag ccgattttgg
    17161 ttaagaatca tggctatgca gatgtttcat atgaagaaac tcctgagcta atcgcggctg
    17221 aaaaagaagc ggctattaag aatcgtctca agctaatcaa tctaccagcc agtctcaaaa
    17281 aagctagttt ggctcaagtt gatttggatg atttgggacg cttgccagtt tttgaaaaac
    17341 tattagcctt tgtggagcaa tatccagcta ttcgaaaagg tctttactta tatggagact
    17401 ttggtgtggg taaaagtttc atggtggcgg ccttagctca tgatttatca gaaaaacgtg
    17461 gtgtttcatc aactctcctc cactatccta gctttgtcat tgatgtcaaa aatgctatca
    17521 gtgatggcaa tgttaagacc ttggtggatg agattaagct gtctgaggtc ctgattttag
    17581 atgatattgg cgccgagcaa tcaaccgctt gggtgcgtga cgaaatcctg caggtcattc
    17641 tccaatatcg gatgcaggaa aatttaccga cctttttcac atccaacttc aactttgaag
    17701 atttggagaa gcattttgcg aaagtgaaac atggaaatga cgaaacctgg gaagccagac
    17761 gggtcatgga acgcatccgt tatttggctg aggagactcg tttagaagga gtaaaccgtc
    17821 gatgacagaa accattaaat tgatgaaggc tcatacttca gtgcgcaggt ttaaagagca
    17881 agaaattccc caagtagact taaatgagat tttgacagca gcccagatgg catcatcttg
    17941 gaagaatttc caatcctact ctgtgattgt ggtacgaagt caagagaaga aagatgcctt
    18001 gtatgaattg gtacctcaag aagccattcg ccagtctgct gttttccttc tctttgtcgg
    18061 agatttgaac cgagcagaaa agggagcccg acttcatacc gacaccttcc aaccccaagg
    18121 tgtggaaggt ctcttgatta gttcggtcga tgcagctctt gctggacaaa acgccttgtt
    18181 ggcagctgaa agcttgggct atggtggtgt gattatcggt ttggttcgat acaagtctga
    18241 agaagtggca gagctcttta acctacctga ctacacctat tctgtctttg ggatggcact
    18301 gggtgtgcca aatcaacatc atgatatgaa accgagactg ccactagaga atgttgtctt
    18361 tgaggaagaa taccaagaac agtcaactga ggcaatccaa gcttatgacc gtgttcaggc
    18421 tgactatgct ggggcgcgtg cgaccacaag ctggagtcag cgcctagcag aacagtttgg
    18481 tcaagctgaa ccaagctcaa ctagaaaaaa tcttgaacag aagaaattat tgtagaaagt
    18541 gagaaattat ggccctacca actattgcca ttgtaggacg tcccaatgtt gggaaatcaa
    18601 ccctatttaa tcggatcgct ggtgagcgaa tctccattgt agaagatgtc gaaggagtga
    18661 cacgtgaccg tatttatgca acgggtgagt ggctcaatcg ttcttttagc atgattgata
    18721 caggaggaat tgatgatgtc gatgctcctt tcatggaaca aatcaagcac caggcagaaa
    18781 ttgccatgga agaagcagat gttatcgtct ttgtcgtgtc cggtaaggaa ggaattaccg
    18841 atgcagacga atacgtagcc cgtaagcttt ataagaccca caaaccagtt atcctcgcag
    18901 tcaacaaggt ggacaaccct gagatgagaa atgatatcta tgatttctat gctctcggtt
    18961 tgggtgaacc attgcctatc tcatctgtcc atggaatcgg tacaggggat gtgctagatg
    19021 cgatcgtaga aaatcttcca aatgaatatg aggaagaaaa tccagatgtc attaagttta
    19081 gcttgattgg tcgtcctaac gttggaaaat caagcttgat caatgctatc ttgggagaag
    19141 accgtgttat tgctagtcct gttgctggaa caactcgtga tgccattgat acccacttta
    19201 cagatacaga tggtcaagag tttaccatga ttgatacggc tggtatgcgt aagtctggta
    19261 aggtttatga aaatactgag aaatactctg ttatgcgtgc catgcgtgct attgaccgtt
    19321 cagatgtggt cttgatggtc atcaatgcgg aagaaggcat tcgtgagtac gacaagcgta
    19381 tcgcaggatt tgcccatgaa gctggtaaag ggatgattat cgtggtcaac aagtgggata
    19441 cgcttgaaaa agataaccac actatgaaaa actgggaaga agatatccgt gagcagttcc
    19501 aatacctgcc ttacgcaccg attatctttg tatcagcttt aaccaagcaa cgtctccaca
    19561 aacttcctga gatgattaag caaatcagcg aaagtcaaaa tacacgtatt ccatcagctg
    19621 tcttgaacga tgtcatcatg gatgccattg ccatcaaccc aacaccgaca gacaaaggaa
    19681 aacgtctcaa gattttctat gcgacccaag tggcaaccaa accaccaacc tttgtcatct
    19741 ttgtcaatga agaagaactc atgcactttt cttacctgcg tttcttggaa aatcaaatcc
    19801 gcaaggcctt tgtttttgag ggaacaccga ttcatctcat cgcaagaaaa cgcaaataaa
    19861 aaagtagaat ctggaatgac agttccggat ttttttgata taataaaata atagaaaacg
    19921 ctatcaaaag aaggagggtg atgtcggtga tggaacattt gtttaaattc ttacttttag
    19981 caccgtactt ttattttgat aactggattg agaaggccaa cagaaatagt aagttttttc
    20041 caatttttta ttatttttac tggttttaca tccccttcta ttctcttttt agccttgctt
    20101 ggacagttgt ttcagttctg tttttcaata ccgtcttgag aaatgtgaca gatatcaagt
    20161 tatggggcat ttggtttctt tttattctgc tagctattgg tatgaattgg ttaacttatt
    20221 cctgtttcaa agaaatgttt cgcttgagac aggaactagg gaagtctaag ggtggaaggc
    20281 attgatttat atcggtttct atttgtagga ggtggcttat ggtacctcta aaatcattta
    20341 ttacacgcta ttccaaggtt tatattggta ataacattta atagaacgaa actaaagaaa
    20401 acaatttaaa agaggaggtt tgtgatggaa tacttgttta aaatgataat cttattaccc
    20461 tgtttttact ttttcagctg gattgaaaag gcaaacagag atagtaagat tttcccaatt
    20521 ttttactatt tttactggat ttacatcacc ctctatgctc tttttagcct tgcttggaca
    20581 gttttttcag ttctgttttt caatatcgtc ttgagaaatt tgacagatat caagttatgg
    20641 ggtatttggc tattattact acttatcgct tttgctagtg actggttaga ctatatctgt
    20701 ttcaaaaaaa tgcttgactt gagacgggaa ctagggaagt ctaaaggcgg aaggcattga
    20761 tttatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttggatt atatatgtaa
    20821 ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcaact tgcggctagt
    20881 ttcctagttt gctctttgat tttcattgag tattatatta ctttctattt gtaggaggtg
    20941 gcttatgaag attcctctct taactttggc aaggcataaa tttgtttatg tcttgcttac
    21001 tttgcttttt cttgctttgg tttatcgtga tgttttgatg acttatttct tttttgatat
    21061 tcatgcgccc gatctagcta aattcgatgg acaagcaatt aaaaatgact tattaaaatc
    21121 agcattagat tttcgtattc tccagttcaa tctaggtttt tatcaatcat ttattattcc
    21181 aatcatcatt gttttgctag gttttcaata tattgagctg aaaaataaag ttttacgatt
    21241 gagtattgga agagaagtga gctatcaagg gttaaaaaga aagttgactt tgcaagttgc
    21301 aagtatccct tgtttgatat atttagtgac tgtgctgata attgcaatta taacctattt
    21361 ctttgggact ttttctcctc ttgaatggaa ttctctattt tctgatggga gtggtttaca
    21421 aagactccta gatggagaga taaaaagcta tttgttcttt acttgtgtcc tactaatcgg
    21481 tattttcatc aatgcaatct attttttaca aatagttgat tatgtgggga atgtgactcg
    21541 ttcggcaatc acctatttga tgtttctttg gcttggttct atgctgctct atagtgcctt
    21601 gccttactat atggttccta tgacgagttt gatgcaagct agctatgggg atgtaagttt
    21661 gatgaaactc tttactcctt atatccttta tgttgtccct tatatggtac ttaaaaaata
    21721 tgaagataat gtttaagaat tttaacaata tttggctaaa gagaaagatc gttttactac
    21781 ttcgtatagt tctgatgatg attttgataa actatctatt gtcaacagcg gttcaaaagc
    21841 aggatgctgt tatctttttc aagagagaat tgatttcaat tttttcctat aatgactatt
    21901 ctgaagcgaa tttagaaatc cccaaactct tgttaaacct ttcgcttttc atggtaggat
    21961 ggctctctgt cattttactt gaaagtgatt tggcagacca ttaccatcac ttgattcgct
    22021 atcaatcaag ctcctttttc gattatacaa ggaaacgatt ggttgtcatt tctaaatttt
    22081 ttactcaaga tttgtttgtc tggtttcttg gtttacttcc gctaggaatt catttcaaaa
    22141 cagtcgcact tttcttttta cttgctcagt taatgatgtt gtacttacta ctgtcttatc
    22201 tgatagcact gattagtgcg ggcgctggtt tttctttttt tctctatttt ttggcatttg
    22261 tgggacaaga atggatgatg gatcatattg taacagtgta tttagtactc ttaagcttat
    22321 tagttatgtt gattgttagt cgcttggaag agaaatttaa gaaaggataa acgatgagac
    22381 ttgaaattat aaatggacag aaaatttatg ggaaaagacc tattttaaat cagttgaatt
    22441 tggtgtttca atcaggaaaa atttatggac ttaaaggtga taatggatct ggcaagacgg
    22501 ttcttttaaa gatacttgct ggttatatta agcttgacaa aggaaaagtt cttcaagatg
    22561 gtaaagttta cggggtaaaa aatcattata ttcaggatgc aggaatttta attgaaaaag
    22621 tcgagttttt atctcattta tccctgagag aaaatttgga actgttaagg tatttttcat
    22681 ctaaagttac ggaaaaaaga attgcctatt ggattcaata ctatgattta caggaatttg
    22741 aagacgttga ataccgtcat ttatccttgg gaacaaagca aaaaatggcc ttgattcaag
    22801 cctttatttc ctctccttct atactctttc tcgatgaacc tatgaatgct ttggatgaga
    22861 agagtgtgag gttaaccaaa caggtcattt tatcttacct gaaaaaagaa aatggtctgg
    22921 ttatcctgac gtcgcacata tcggaagata tttcagacct ttgtacagat gtattagttg
    22981 tcgaaaatgg acatatacaa tatgtaaagg atatacaatc ctaggagatg gcttatggca
    23041 catctaaaat catttattac acgctattcc aaggtttata ttggtttagt tctgctgatc
    23101 tggctgtctt tcttctttat cccttgggat agagcgattc tgggggtaag gattgacatc
    23161 ttcatcatgc agaaaatctt gctagctttt ggaattctgt ccattctcat ggccttgctg
    23221 tccaagaaag tcagtctctt tgtttttgga ctgatttgct gtctttctct ttggattaac
    23281 ttatttatca catttgccat tttgccgatt tttggcaatt aaacagtcat aaaagtcgga
    23341 gaggttagct tgaaaactaa cctctttttc cttttcaaaa tggggattct tccttgaaaa
    23401 taatcagtaa ttgtgctaaa attaaaggaa cattctaaaa tattcggaat ttaaagtaag
    23461 gaaaaacatg gctaatattt taaaaacaat tatcgaaaat gataaaggag aaatccgtcg
    23521 tctggaaaag atggctgaca aggttttcaa atacgaagac caaatggcta ctttgactga
    23581 cgaccaacta aaagcaaaaa cagttgaatt taaggaacgt tatcaaaatg gagaatcact
    23641 ggattcattg ctttacgaag catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg
    23701 tctcttccct tataaggttc aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc
    23761 agagatgcgt acaggggaag ggaaaacctt gactgcgacc atgccggtat acctcaatgc
    23821 cctttcaggt aaaggggttc acgtagttac ggttaatgaa tacctgtcag aacgtgacgc
    23881 gactgagatg ggtgaattgt actcttggct tggtttgtca gtagggatta acttggctac
    23941 caaatctcca atggagaaaa aagaagccta tgagtgtgat attacttact caactaactc
    24001 agaaatcgga tttgactacc ttcgtgacaa catggtcgtt cgcgccgaaa acatggtaca
    24061 acgtccgctt aactatgcct tggtcgatga ggttgactct atcttgattg acgaggctcg
    24121 tacacctttg attgtatcag gtgccaatgc ggttgaaacc agtcagttgt atcacatggc
    24181 agaccactat gtaaaatctt tgaacaaaga tgactacatc atcgatgtgc agtctaagac
    24241 tattggtttg tctgattcag ggattgacag ggctgaaagc tacttcaaac ttgaaaacct
    24301 ctatgacatc gaaaacgtgg ctttgactca ctttatcgat aacgcccttc gtgccaacta
    24361 catcatgctt ctcgatattg actatgtggt gagcgaagag caagaaatct tgattgtcga
    24421 ccaatttaca ggtcgtacca tggaaggtcg tcgttattct gatggattgc accaagctat
    24481 tgaagccaaa gaaggtgtgc caatccagga tgaaaccaag acatctgcct caatcacgta
    24541 ccaaaacctt ttccgtatgt acaagaaatt gtctggtatg acgggtacag gtaagactga
    24601 ggaagaagaa ttccgtgaaa tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc
    24661 tgttcaacgt attgaccact cagaccttct ttatgcaagt atcgaatcta agtttaaagc
    24721 ggttgtcgaa gacgttaagg ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt
    24781 agcggttgaa actagtgact acatttctaa gaaattggtt gcagctggtg ttcctcacga
    24841 agtcttgaat gccaaaaacc actatagaga agcccaaatc atcatgaatg ctggtcaacg
    24901 tggtgccgtt accatcgcaa ccaacatggc gggtcgtggt accgacatca agcttggtga
    24961 aggtgttcgt gaacttggag gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg
    25021 tatcgataac cagcttcgtg gacgttcagg tcgtcaagga gatccaggtg agtcacaatt
    25081 ctacctatct cttgaagatg atttgatgaa acgttttggt tctgaacgct tgaagggaat
    25141 ctttgaacgt ttgaacatgt ctgaagaggc gattgagtct cgcatgttga cgcgtcaggt
    25201 tgaagcagct cagaaacgtg tcgaaggaaa taactacgat acccgtaaac aagtccttca
    25261 atacgatgat gtcatgcgtg aacaacgtga gattatctat gctcaacgtt acgatgtcat
    25321 cactgcagat cgtgacttgg cacctgaaat tcaggctatg atcaaacgca cgattgaacg
    25381 tgtcgttgat ggtcatgcgc gtgccaaaca agatgaaaaa ctagaggcaa ttttgaactt
    25441 tgctaagtac aacttgcttc ctgaagattc tattacgatg gaagacttgt caggcttgtc
    25501 tgataaggcc atcaaggaag agcttttcca acgtgccttg aaggtttacg atagtcaggt
    25561 ttcaaaacta cgcgatgaag aagcagttaa agaattccaa aaagttttga ttctacgagt
    25621 ggtggataac aagtggacag atcatatcga tgcccttgat caattgcgta acgcggttgg
    25681 acttcgtggc tatgctcaga acaaccctgt tgttgagtat caggcagaag gtttccgtat
    25741 gtttaatgat atgattggtt cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca
    25801 aattcatgaa caagaaagac cacaggcaga acgtcatatc agtacaacag cgactcgcaa
    25861 tatcgctgct caccaagcaa gtatgccaga agatttggat ttgagccaga ttggacgcaa
    25921 tgaactttgc ccatgtggtt ctggtaagaa atttaaaaac tgtcacggta aaagacaata
    25981 aaatgagata gtttagaggc ggatatcttg tgaaaagtaa atttttactg ggtatccgtt
    26041 tgctttataa ggagatgagt tatggtattt acagcaaaaa gctctaaaat aaatatagaa
    26101 gaagttcgtg ccttgtcaaa attagaaggt caggctttgg agaggaaatc acagcgcgat
    26161 caagagctag aagccattat acgtggagaa gatcagcgaa ttctcttggt aatcgggcca
    26221 tgctcatctg acaacgaaga agctgtcctt gaatacgcta agcgtttggc agtcctacaa
    26281 gaagaagtgg cagatcgtat ctttatggtt atgcgtgttt atactgccaa accccgtacc
    26341 aacggagatg gctataaggg cttgattcac cagcctaacg cgacagaagc gcctagtctt
    26401 atcaatggaa tcaaagccgt tcgccatctt cactatcgtg tcatcacaga aacagggatg
    26461 acaactgctg atgaaatgct ttatcctgaa aaccttccgc ttgtagatga tttgatttct
    26521 tacatggcag ttggtgcccg ttcagttgaa gaccagcaac accgctttgt ggcaagtggg
    26581 gcaggatttt ctactggttt taaaaatcca acctctggaa atctcaatgt catgtttaat
    26641 gggatttatg ctgctcaaaa caaacaaagt ttccttttct taggaaaaga agtagaaaca
    26701 actgggaacc cgctttcaca cgctattctt cgtggtgctc ttaatgagta tggaaaaaat
    26761 attcccaact actattatga caatttaatt gataccattg cccaatatga gaaaatgggc
    26821 ttggaaaatc cttttatcat cattgatacc aatcatgaca attctggtaa gcagtatatt
    26881 gaacagatcc gaattgtccg ccagaccttg attaaccgtg cttggaatga aaaaattaag
    26941 cagttcgttc gtggttttat gattgagtct tatctggaag atggtcgaca aaatgagcca
    27001 gaagtatttg gcaagtctat cacagaccct tgcctgggtt gggataacac agaagctctt
    27061 gtcagagaaa tttacaaaac gttaggagaa taagatggca tttattgaaa aaggtcaaga
    27121 aatcgatatg gaagtcatca aggctgaaac ccaattgtct gcggaagcct tgagactcaa
    27181 ggaaagccgt gacagggaat tggcagatat tatttcaggg gaagatgacc gtattctctt
    27241 ggtgattggt ccttgctctt ctgataatga agaggcggtc ttggaatatg ctcgccgttt
    27301 atccgccttg caaaagaagg tagcggacaa gattttcatg gtcatgcgcg tgtatactgc
    27361 taagcctcgt accaatggag acggctataa aggattggtt caccagccag atacttctaa
    27421 ggctccaagc ctgattaacg gtttgcaggc tgtgcgccag ttgcattacc gcgtgattac
    27481 agagactggt ttgacaacgg cagatgagat gctttatccg tcaaatctga tcttggtgga
    27541 tgacttggtc agctaccatg ccgttggagc tcgttctgtg gaagaccaag agcaccgctt
    27601 tgtggcttct gggattgatg caccagtagg gatgaaaaat ccaacctcag gaaatttggg
    27661 tgttatgttt aacgccatct atgctgctca aaacaagcaa accttccttt atcatgggca
    27721 ggaagttgag acatcaggta atcctttggc ccatgttatc ctccgtggag cagtcaacga
    27781 gtatggcaat tatatgccga attactacta tgaaaaccta ctccaagcca ttgaacgcta
    27841 tgaaaccatg ggacttgaaa atccttttat cctcattgac accaaccatg ataactcagg
    27901 caagcaatat atggagcaga ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa
    27961 tgagaaaatt aaaaagacgg ttcgaggatt tatgattgaa tcttacctag cagatggtcg
    28021 tcaaaaccaa ccagagatct ttggttgctc tattactgac ccttgcctag gttgggaaaa
    28081 tacagaggcc ttggtagaag agatttatgt taccttgaca aaataagtga aaaggatgga
    28141 gttggggaat ctcaactcct tttgatgaga atgatagttg gacacggaat tgacatcgaa
    28201 gaattggctt cgatagaaag cgcagttaca cgacatgaag gatttgctaa gcgtgtactg
    28261 accgctcagg aaatggagcg cttcaccagt ctcaaaggac gcaggcaaat agaatattta
    28321 gctggtcgct ggtcggctaa ggaggccttt tccaaggcta tgggaacggg cattagcaag
    28381 ctcggttttc aggatttgga agtattgaac aatgaacgtg gggcgcctta ttttagtcag
    28441 gcaccatttt caggaaagat ttggctgtct atcagccaca ccgatcagtt tgtgacagcc
    28501 agtgtcattt tggaggaaaa tcatgaaagc tagtccacat agaccaacca aggctctgat
    28561 tcatctggga gctattcgac aaaatattca gcaaatgggg gctcatatcc ctcaaggaac
    28621 gctcaagttg gctgtggtta aggccaatgc ttatggtcat ggagctgttg ccgttgccaa
    28681 ggcaattcaa gatgatgttg atggcttttg cgtttccaat atcgatgaag ccattgaact
    28741 cagacaagct ggactcagca agccaatcct cattttagga gtttctgaaa tcgaagctgt
    28801 tgctctagct aaagaatatg acttcacctt gacagtggct ggactggagt ggattcaagc
    28861 actcttagat aaggaagtgg acctaactgg attgacagtc cacctcaaga ttgattcagg
    28921 gatgggacgg attggtttta gagaggcaag tgaggttgag caggctcaag atttgctcca
    28981 acaacacggt gttcgtgttg aaggaatctt tacccacttt gctactgctg atgaggaatc
    29041 agatgactat tttaatgccc agttagaacg gtttaaaact attttagcta gtatgaagga
    29101 agttccagag ctggttcatg ctagcaattc tgcaacgact ctttggcatg tagagactat
    29161 tttcaatgcg gttcgtatgg gagatgccat gtatggtctc aatccaagtg gagcggtctt
    29221 ggctttgcct tatgatttga taccggcctt gaccttggag tctgctctgg ttcatatcaa
    29281 gacagttcca gctggtgctt gcatgggcta tggagcaact tatcaaacgg atagcgagca
    29341 agtcatcgcg accgtgccaa tcgggtatgc agatggatgg acaagagaca tgcaaaattt
    29401 ctctgtcttg gtagatggcc aagcttgccc aattgtcggc agggtttcga tggaccaaat
    29461 cactattcga ttgcctaagc cttacccact cggaaccaag gtaaccttga ttggctccaa
    29521 tggggataag gaaatcactg caactcaggt agcgacctac cgcgtaacca ttaactatga
    29581 ggtggtttgc ctcctcagcg accgtattcc gagagaatat tactagaaaa gaaaggagtg
    29641 gagcatgaat ctacatcaac ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga
    29701 aaaatacgcc aaactaggaa ttgaaaactt gcaagatctc ttgctctact ttcctttccg
    29761 ttatgaagac ttcaaaatca agcaggtgct ggagctggaa gacggtgaga aggcagttct
    29821 ttctggtcag gtagtgactc ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct
    29881 gcgttttagt ctcaagcagg gagaggtcgt ttttgcggtg aatttcttta accagcccta
    29941 tctggctgat aaaatagagt tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc
    30001 taaggctagt ctgactggga tgaaggttct ggctcaggta gaagatgacc tccagcctgt
    30061 ctatcgtctg gctcagggaa tcagtcaggc cagtctggtt aaggtcatca agacggcttt
    30121 tgatcaggga ctggacctct tgatagaaga aaatctgccc cagtctttac tagacaaata
    30181 caaactcatg tcccgttgtc aggcagtccg agctatgcat tttccaaagg atttggcaga
    30241 atacaagcag gctcttcgcc gtatcaagtt tgaggaactc ttttatttcc aaatgcagct
    30301 gcagatgctc aagtctgaaa atagagttca gggaagtggt ctggttctga attggtctca
    30361 ggaaaaagtg acagcagtta aagtaagtct tccttttgcc ctgacccaag ctcaggaaaa
    30421 gagtttgcag gaaattttaa ctgatatgaa gtccgaccac cacatgaatc gtctcctaca
    30481 aggggatgtg gggagtggaa aaacggtagt cgctggcttg gccatgtttg cggcagtgac
    30541 agcaggttat caggctgccc taatggtacc aacagaaatc ctcgcagagc aacactttga
    30601 gagtttacag aacctttttc ccaatttgaa actggctctc ttgacaggtt ccttgaaagc
    30661 tgcagaaaag agagaggtct tggagaccat tgccaagggt gaggctgatt tgattatcgg
    30721 aactcacgct ctgatacaag atggggtgga gtatgctcgt cttggtttga ttattatcga
    30781 tgagcagcac cgttttggtg tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc
    30841 agatgtcctc atgatgacgg cgactcccat tccacggacg cttgccatca cagcctttgg
    30901 agatatggat gtttccatta tcgaccagat gccagcaggt cggaagccta ttgtgacgcg
    30961 ctggatcaaa catgagcaac tacctcaggt cttgacttgg ttagaggggg aaattcaaaa
    31021 aggttcccaa gcctatgtca tctctccttt gattgaagaa tcagaagctc tagatttgaa
    31081 aaatgccatt gccttatcag aggagttgac gactcatttt gcaggcaagg cagaggtggc
    31141 tcttctacat ggtaggatga agagtgacga aaaagaccag atcatgcagg atttcaagga
    31201 gagaaagacg gatattctgg tttcgacgac ggttattgag gttggggtca acgttcccaa
    31261 tgcgactgtc atgattatca tggatgccga tcgcttcggt ctcagtcaac ttcaccagct
    31321 tagaggtcgt gtcggtcggg gggacaagca gtcctacgct gttctcgttg ccaatcccaa
    31381 gacggattct gggaaagacc gcatgcgtat catgacagaa acgaccaatg gatttgtcct
    31441 tgcggaggaa gatttgaaaa tgcgtggttc tggtgagatt tttggaacca gacagtcagg
    31501 acttccagag ttccaagtgg ctgatattat cgaagatttt ccgattttag aagaagcaag
    31561 aaaggttgct agctacatta gttctataga agcttggcaa gaagatccag agtggcgcat
    31621 gattgccctt catctggaaa agaaagaaca tctggattaa gctttctcta aggaaaactt
    31681 aagctagttt ttaggtttgg ctcttatact aaagtcatca aaaagaaacg aggactctca
    31741 tatgacagta acgattaaag taaattacca aaccactttc caaaagaagg aagcaaaaaa
    31801 ctagtataaa cagaagagag agcgaaatgc tctttttcgt ttctaaaact actttcagcc
    31861 catcatccta aaagtaaaga atctaaattc actttctatt tatccttctt tcttgcattg
    31921 attacataga tatgctacag ttgtggtaac gattacaaaa taaaaggagc atgctatgaa
    31981 aaatccagct ttgctagaag aaattaagac ctatagagga agggatgagg ttccggaaga
    32041 ctttgatgct ttctgggatg gggaagtgaa aaatgtttcc acgcttccat cctaccactt
    32101 ggaggaaaga gatttccaca ttcctcaagt caagtgctat gagttaacat ttgaaggaag
    32161 caaggaagga aaggtctatg cacgcattgt tcttccaaag agtgaggaga aggttccact
    32221 aatcttccat tttcatggtt atatgggacg tggctgggac tgggccgaca tgctgggctt
    32281 caccgtagct ggttacggtg ttgtttccat ggatgtgcgg ggccagtcag gttactcaca
    32341 agacggcttg cgttctcctt taggaaatac cgtgaagggg catattatcc gtggtgctat
    32401 ggaaggtcgg gaccacctct tttataagga tgtttatctg gatatttacc agttggtcga
    32461 aattgttgct agtctgtctc aggttgatga gaagcgtctt tctagctatg gtgcctcaca
    32521 aggaggggct ctagctctag ttgcagcagc gctcaatcct cgaattcaga aaacagttgc
    32581 catttatccc ttcttgtcag acttcagacg ggtgattgag attggtaata ctagcgaggc
    32641 ttacgacgaa cttttccgtt atttcaagtt tcacgacccc ttccatgaaa cagaggagga
    32701 aatcatggcg acccttgcct atatcgatgt caaaaatctt gcccatcgta tccaaggtga
    32761 ggttaagatg attacgggct tggacgacga tgtttgctat cccattaccc agtttgcgat
    32821 ttataatcgt ctgacctgcg ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc
    32881 catgaatgta tttgtcaatg accaagtcta caactggctc tgtggaagtg agattccttt
    32941 taaatatcta aaataaggag tcgactctaa gcacaaaatc ttaaaaatta caaacacgca
    33001 tagtatcagg ggattaaaaa aacttgatac tatgcgtttt atcatggaaa tatagtaaaa
    33061 tgaaacaaga ataggacaaa acgatcagga cagtcaaatc gatttctaac aatgttttag
    33121 aagcaaatgt gtactattct agtttcaatc tattatattt atagaatttt ttgttgctag
    33181 atttgtcaaa ttgcttaaaa taattttttt cagaaagcaa aagccgatac ctatcgagta
    33241 gggtagttct tgctatcgtc aggcttgtct gtaggtgtta atacttttca aaaatctctt
    33301 taaaccacgt cagcttcgcc ttgccgtata tatgttactg acttcgtcag ttctatctgc
    33361 cacctcaaaa cggtgttttg agctgacttt gtcagtctta tctacaacct caaaacagtg
    33421 ttttgagcat catgcggcta gcttcttagt ttgctctttg attttcattg agtataaaaa
    33481 cagatgagtt tctgttttct ttttatggac tataaatgtt cagctgaaac tactttcaag
    33541 gacattatta tataaaagaa ttttttgaaa ctaaaatcta ctatattaca ctatattgaa
    33601 agcgttttaa aaatgaggta taataaattt actaacgctt ataaaaagtg atagaatcta
    33661 tctttatgta tatttaaaga tagattgctg taaaaatagt agtagctatg cgaaataaca
    33721 gatagagaga agggattgaa gcttagaaaa ggggaataat atgatattta aggcattcaa
    33781 gacaaaaaag cagagaaaaa gacaagttga actacttttg acagtttttt tcgacagttt
    33841 tctgattgat ttatttcttc acttatttgg gattgtcccc tttaagctgg ataagattct
    33901 gattgtgagc ttgattatat ttcccattat ttctacaagt atttatgctt atgaaaagct
    33961 atttgaaaaa gtgttcgata aggattgagc aggaagtatg gtataaatag cataagctga
    34021 tgtccatcat ttgcttataa agagatattt tagtttaatt gcagcggtgt cctggtagat
    34081 aaactagatt ggcaggagtc tgattggaga aaggagaggg gaaaattggc accaatttga
    34141 gatagtttgt ttagttcatt tttgtcattt aaatgaactg tagtaaaaga aagttaataa
    34201 aagacaaact aagtgcattt tctggagtaa atgtcttatt tcagaaatcg ggatatagat
    34261 atagagagga tcagtatgaa tcggagtgtt caagaacgta agtgtcgtta tagcattagg
    34321 aaactatcgg taggagcggt ttctatgatt gtaggagcag tggtatttgg aacgtctcct
    34381 gttttagctc aagaaggggc aagtgagcaa cctctggcaa atgaaactca actttcgggg
    34441 gagagctcaa ccctaactga tacagaaaag agccagcctt cttcagagac tgaactttct
    34501 ggcaataagc aagaacaaga aaggaaagat aagcaagaag aaaaaattcc aagagattac
    34561 tatgcacgag atttggaaaa tgtcgaaaca gtgatagaaa aagaagatgt tgaaaccaat
    34621 gcttcaaatg gtcagagagt tgatttatca agtgaactag ataaactaaa gaaacttgaa
    34681 aacgcaacag ttcacatgga gtttaagcca gatgccaagg ctccagcatt ctataatctc
    34741 ttttctgtgt caagtgctac taaaaaagat gagtacttca ctatggcagt ttacaataat
    34801 actgctactc tagaggggcg tggttcggat gggaaacagt tttacaataa ttacaacgat
    34861 gcacccttaa aagttaaacc aggtcagtgg aattctgtga ctttcacagt tgaaaaaccg
    34921 acagcagaat tgccaaatgg tcgagttcgc ctgtatgtta atggtgtttt atctcgaaca
    34981 agtctgaaat ctggcaattt cattaaagat atgccagatg taacgcatgt gcaaatcgga
    35041 gcaaccaagc gtgccaacaa tacggtttgg gggtcaaatc tacagattcg gaatctcact
    35101 gtgtataatc gtgctttaac accagaagag gtacaaaaac gtagtcaact ttttaaacgc
    35161 tcagatttag aaaaaaaact acctgaagga gcggctttaa cagagaaaac ggacatattc
    35221 gaaagcgggc gtaacggtaa accaaataaa gatggaatca agagttatcg tattccagca
    35281 cttctcaaga cagataaagg aactttgatc gcaggtgcag atgaacgccg tctccattcg
    35341 agtgactggg gtgatatcgg tatggtcatc agacgtagtg aagataatgg taaaacttgg
    35401 ggtaaccgag taaccattac caacttacgt gacaatccaa aagcttctga cccatcgatc
    35461 ggttcaccag tgaatatcga tatggtgttg gttcaagatc ctgaaaccaa acgaatcttt
    35521 tctatctatg acatgttccc agaagggaag ggaatctttg gaatgtcttc acaaaaagaa
    35581 gaagcctaca aaaaaatcga tggaaaaacc tatcaaatcc tctatcgtga aggagaaaag
    35641 ggagcttata ccattcgaga aaatggtact gtctatacac cagatggtaa ggcgacagac
    35701 tatcgcgttg ttgtagatcc tgttaaacca gcctatagcg acaagggtga tctatacaag
    35761 ggtaaccaat tactaggcaa tatctacttc acaacaaaca aaacttctcc atttagaatt
    35821 gccaaggata gctatctatg gatgtcctac agtgatgacg acgggaagac atggtcagcg
    35881 cctcaagata ttactccgat ggtcaaagcc gattggatga aattcttggg tgtaggtcct
    35941 ggaacaggaa ttgtacttcg gaatgggcct cacaagggac ggattttgat accggtttat
    36001 acgactaata atgtatctca cttaaatggc tcgcaatctt ctcgtgtcat ctattcagat
    36061 gatcatggaa aaacttggca tgctggagaa gcggtcaacg ataaccgtca ggtagacggt
    36121 caaaagatcc actcttctac gatgaacaat agacgtgcgc aaaatacaga atcaacggtg
    36181 gtacaactaa acaatggaga tgttaaactc tttatgcgtg gtttgactgg agatcttcag
    36241 gttgctacaa gtaaagacgg aggagtgact tgggagaagg atatcaaacg ttatccacag
    36301 gttaaagatg tctatgttca aatgtctgct atccatacga tgcataatgg aaaagaatac
    36361 attatcctca gcaatgcagg tggaccgaat cgtgaaaatg ggatggtcca cttggcacgt
    36421 gtcgaagaaa atggtgagtt gacttggctc aaacacaatc caattcaaaa aggagagttt
    36481 gcctataatt cgctccaaga attaggaaat ggggagtatg gcatcttgta tgaacatact
    36541 gaaaaaggac aaaatgccta taccctatca tttagaaaat ttaattggga atttttgagc
    36601 aaagatctga tttctcctac cgaagcgaaa gtgaagcgaa ctagagagat gggcaaagga
    36661 gagatgggca aaggagttat tggcttggag tttgactcag aagtattggt caacaaggct
    36721 ccaacccttc aattggcaaa tggtaaaaca gcgactttcc taacccagta tgatagcaaa
    36781 accttgttgt ttgcagtaga taaggaagat atcggacagg aaattattgg tatagctaaa
    36841 ggaagcatcg aaagtatgca taatcttcct gtaaatctag caggtgccag agttcctggc
    36901 ggagtaaatg gtagcaaagc agcggtgcat gaagttccag aatttacagg gggagttaat
    36961 ggtacagagc cagctgttca tgaaatcgca gagtataagg gatctgattc gcttgtaact
    37021 cttactacaa aagaagatta tacttacaaa gctcctcttg ctcagcaggc acttcctgaa
    37081 acaggaaaca aggagagtga cctcctagct tcactaggac taacagcttt cttccttggt
    37141 ctgtttacgc tagggaaaaa gagagaacaa taagagaaga attctaaaca tttgattttg
    37201 taaaaataga aggagatagc aggttttcaa gcctgctatc tttttttgat gacattcagg
    37261 ctgatacgaa atcataagag gtctgaaact actttcagag tagtctgttc tataaaatat
    37321 agtagattga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaaa
    37381 attttagcag tcaaggtgta ctgttataga ttcaatatat tatgtgacta ttaaccttct
    37441 cttctcctaa aattgacttt cttgttttct tatcttgtcc actcgaaaca agtattgtaa
    37501 gaatttgatt atttttgaaa gtacttttaa tatacttgat atagttaaaa aagatttgaa
    37561 actaaattcc aaattagaaa aagacttgaa atactaaaaa aaaagtatac tctaattgaa
    37621 aacggtaaca aaactaatt
//
LOCUS       DAVBCR010000011        37445 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCR010000011 DAVBCR010000000
VERSION     DAVBCR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.011"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ508_000508"
     CDS             complement(<1..777)
                     /locus_tag="VQ508_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2489559.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ508_000509"
     CDS             complement(873..2183)
                     /locus_tag="VQ508_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2489560.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ508_000510"
     CDS             complement(2310..3305)
                     /locus_tag="VQ508_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2489561.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ508_000511"
     CDS             complement(3667..5148)
                     /locus_tag="VQ508_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2489562.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ508_000512"
     CDS             5591..5755
                     /locus_tag="VQ508_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2489563.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ508_000513"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ508_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2489564.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ508_000514"
     CDS             6417..6587
                     /locus_tag="VQ508_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2489565.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6618..7226
                     /locus_tag="VQ508_000515"
     CDS             6618..7226
                     /locus_tag="VQ508_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2489566.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7257..7460
                     /locus_tag="VQ508_000516"
     CDS             7257..7460
                     /locus_tag="VQ508_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2489567.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7570..7848)
                     /locus_tag="VQ508_000517"
                     /pseudo
     CDS             complement(7570..7848)
                     /locus_tag="VQ508_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8046..8333
                     /locus_tag="VQ508_000518"
     CDS             8046..8333
                     /locus_tag="VQ508_000518"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2489568.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8611..9381)
                     /locus_tag="VQ508_000519"
     CDS             complement(8611..9381)
                     /locus_tag="VQ508_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2489569.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9396..10244)
                     /locus_tag="VQ508_000520"
     CDS             complement(9396..10244)
                     /locus_tag="VQ508_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2489570.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10241..11716)
                     /locus_tag="VQ508_000521"
     CDS             complement(10241..11716)
                     /locus_tag="VQ508_000521"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2489571.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12039..13394)
                     /locus_tag="VQ508_000522"
     CDS             complement(12039..13394)
                     /locus_tag="VQ508_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2489572.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13404..13721)
                     /locus_tag="VQ508_000523"
     CDS             complement(13404..13721)
                     /locus_tag="VQ508_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2489573.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13738..14055)
                     /locus_tag="VQ508_000524"
     CDS             complement(13738..14055)
                     /locus_tag="VQ508_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2489574.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14068..15753)
                     /locus_tag="VQ508_000525"
     CDS             complement(14068..15753)
                     /locus_tag="VQ508_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2489575.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15758..16654)
                     /locus_tag="VQ508_000526"
     CDS             complement(15758..16654)
                     /locus_tag="VQ508_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2489576.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16657..17919)
                     /locus_tag="VQ508_000527"
     CDS             complement(16657..17919)
                     /locus_tag="VQ508_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2489577.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            18199..19377
                     /locus_tag="VQ508_000528"
     CDS             18199..19377
                     /locus_tag="VQ508_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2489578.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(19605..20051)
                     /locus_tag="VQ508_000529"
     CDS             complement(19605..20051)
                     /locus_tag="VQ508_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489579.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            20121..20780
                     /locus_tag="VQ508_000530"
     CDS             20121..20780
                     /locus_tag="VQ508_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2489580.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            complement(20832..21983)
                     /locus_tag="VQ508_000531"
     CDS             complement(20832..21983)
                     /locus_tag="VQ508_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2489581.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(21998..22552)
                     /locus_tag="VQ508_000532"
     CDS             complement(21998..22552)
                     /locus_tag="VQ508_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489582.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            complement(22589..22729)
                     /locus_tag="VQ508_000533"
     CDS             complement(22589..22729)
                     /locus_tag="VQ508_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489583.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            22958..23059
                     /locus_tag="VQ508_000534"
                     /pseudo
     CDS             22958..23059
                     /locus_tag="VQ508_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(23031..23807)
                     /locus_tag="VQ508_000535"
     CDS             complement(23031..23807)
                     /locus_tag="VQ508_000535"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2489584.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(23800..25023)
                     /gene="trpB"
                     /locus_tag="VQ508_000536"
     CDS             complement(23800..25023)
                     /gene="trpB"
                     /locus_tag="VQ508_000536"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2489585.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(25001..25600)
                     /locus_tag="VQ508_000537"
     CDS             complement(25001..25600)
                     /locus_tag="VQ508_000537"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2489586.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(25587..26354)
                     /gene="trpC"
                     /locus_tag="VQ508_000538"
     CDS             complement(25587..26354)
                     /gene="trpC"
                     /locus_tag="VQ508_000538"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2489587.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(26351..27355)
                     /gene="trpD"
                     /locus_tag="VQ508_000539"
     CDS             complement(26351..27355)
                     /gene="trpD"
                     /locus_tag="VQ508_000539"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2489588.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(27366..27932)
                     /locus_tag="VQ508_000540"
     CDS             complement(27366..27932)
                     /locus_tag="VQ508_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2489589.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(27929..29290)
                     /gene="trpE"
                     /locus_tag="VQ508_000541"
     CDS             complement(27929..29290)
                     /gene="trpE"
                     /locus_tag="VQ508_000541"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2489590.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(29791..29868)
                     /locus_tag="VQ508_000542"
                     /pseudo
     CDS             complement(29791..29868)
                     /locus_tag="VQ508_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30473..31474)
                     /locus_tag="VQ508_000543"
     CDS             complement(30473..31474)
                     /locus_tag="VQ508_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2489591.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(31582..31950)
                     /locus_tag="VQ508_000544"
     CDS             complement(31582..31950)
                     /locus_tag="VQ508_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489592.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(32046..32756)
                     /locus_tag="VQ508_000545"
     CDS             complement(32046..32756)
                     /locus_tag="VQ508_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2489593.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(32759..34450)
                     /locus_tag="VQ508_000546"
     CDS             complement(32759..34450)
                     /locus_tag="VQ508_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2489594.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(34462..35472)
                     /locus_tag="VQ508_000547"
     CDS             complement(34462..35472)
                     /locus_tag="VQ508_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489595.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(35576..36643)
                     /locus_tag="VQ508_000548"
     CDS             complement(35576..36643)
                     /locus_tag="VQ508_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2489596.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            complement(36668..37327)
                     /locus_tag="VQ508_000549"
     CDS             complement(36668..37327)
                     /locus_tag="VQ508_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2489597.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
BASE COUNT        12036 a         7633 c         5732 g        12044 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa ttactaattt
     6601 aattaaagga gtttcatatg tcaaacgaaa aaaacacaaa tactaacgta gaaaagaaag
     6661 atgctactgt tgtagctcac gaaatcaaag gggaacttac ttacgaagat aaagttatcc
     6721 aaaaaatcat tggtctttca ctagaaaacg tttcaggtct tttgggaatc gatggtggtt
     6781 tcttctcaaa tcttaaagaa aaaatcgtta acagcgatga cgtaacaagt ggtgttaacg
     6841 tagaagttgg taaaacacaa gttgcagttg acttaaacgt tattgttgag taccaaaaaa
     6901 atgttccagc tttatattca gaaatcagag aaatcgtatc ttcagaagtt gctaaaatga
     6961 ctgacttgga aattgttgaa atcaacgtaa acgttgtcga catcaaaact aaagaacagc
     7021 atgaagcaga ctcagtaagc cttcaagatc gcgtatctga cgttgctgaa tcaacaggag
     7081 aattcacttc agaacaattc gaaaaagcta aatctggtct tggatctggt ttctcaactg
     7141 ttcaagaaaa agttagcgaa ggtgtagaag ctgttaaagg tgcagcaaat ggtgtagtat
     7201 ctcacgaaaa cactcgtgta aactaagata aaataaatat aacaggagaa attatcatgt
     7261 cagtagaaga aaaattaaat caagctaaag gttctattaa agaaggtgtt gggaaagcca
     7321 tcggtgatga aaaaatggaa aaagaaggag cagctgaaaa agttgtttct aaagtaaaag
     7381 aagttgccga agacgctaaa gacgctgtag aaggtgctgt agaaggtgtt aaaaacatgt
     7441 tgagtggcga cgataaataa ggttaaaagt tactttatct ttttggtaac attagtcaaa
     7501 agagtctgag tcaagatgat tctcagaaaa caaaaagcta gagattccca attgcggaac
     7561 tctagctttt taattttgcc tctttctctt attatatttc agcaggttgt tggccatgag
     7621 tatgaatccc atgtcaattc tcacttgacg cttacctctc agatgacatc tcttataacc
     7681 caaacaaacc tttatctgcc caaagacaga tttcatatca atcttacgtt tagcgaaaat
     7741 ttgtctaccc ttggaagata aaagtgcctg atattcttta gtttttaaac actggtaacg
     7801 ttcattcata tacagtccct tttgaggggc tgattcaggt tcataatcgc agtcaacatt
     7861 gatttcaagg ctgtttgctt tctatctccc ctttttcatg atatattttc gtacaggttc
     7921 ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca cgttgcggta taatgttctt
     7981 gtggcatatt cattagattt tcgtaaacag ttctctccta tttgtgagcg aacaagtata
     8041 gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc aaaggatatg gcagaatcca
     8101 tccaagggtg gctggcacaa tttctagtga acctctttaa atctattacg tttgactgtg
     8161 gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca cgatagtgag agcttttttg
     8221 cgaatctagg atgttcaaga cagcggcgtc tcaacgaaca ttcaaataga cttttaaggt
     8281 gtcatgactt gccaaagcag acagatttca acgaagtcag tcaagaattt taggctggac
     8341 aaaaactcaa tttcaggaga aaatgaaata aatattccca caataaaacg catagtatca
     8401 aagtttttcc aacacctgat attatgcatt ttttaatttt aaagactttt tcccagcctc
     8461 aagaaatcac atcccaccta aatcctttgg ataccaaata cctcatcagg tattttctga
     8521 gttggtaaga atgtctagct taatttgaca aatggagggc gttaaatcca gtttttacag
     8581 gaagtctatt taaatatttt cacactgacc ttacaaaata tgaaataaat tatataaagt
     8641 cattcccatg acaaagaata acatcccttt aaaacagtat cttacccaat tttcaggtaa
     8701 gacttttgat acaaccgtcc ccaatacccc tccgcatata gaagcaagaa aaataactgg
     8761 aacaggtgct aaatgatact gatttaaacc tgtaaccaca acaattgtag ccaaatgaga
     8821 cagctgggaa aagaaaataa ttgcaagaga atacatcgtt gcttctttta tcgaaataga
     8881 aaaaaacacc atcaataaag atacattgag cggaccacca ccaatcccta aaaaactaga
     8941 aattaagccc aatagaagac cacaaatcat atagaatcct atcttatcaa attgataagt
     9001 ttttttaaaa tctgttaaaa caaatgatac aagtagcatc acaaatagta aaatcatttg
     9061 aacaatggat actagccgta ctgataactg agataacaaa acttgaaaaa taagatgacc
     9121 tagaaatcct cctaaaacag aagaaaagga taccgtaaaa atcaatctcc attttatctc
     9181 tttactttgt gaaaatcttt tgattgttga aataatcgcc attataaaaa cagagaaact
     9241 agaataaaaa gcaatatcac tgaccgaatg gtatccaaaa ctatccataa gtggcttaat
     9301 tataattcca ccacccatgc ctgatataga acccaatcca ctagctatta taatgattag
     9361 aaaataaata atctcaacca ctttttaagt ccatcctatt ctttcaaaca tctaaaacct
     9421 agatggctac tagtggaagt agagatacat ccatttctag cagctactcg gtatcgatta
     9481 caataagaac aatgacataa aaaggaacct cctctgatag catactcctc atcttgtatc
     9541 ccatattttg gcaactcata gtcatcttca ttaaaactag ctaataaggt atatctcgga
     9601 ttgcgacacc attcccaaac attcccaatc atttgatata gaccaaaatc attaggtaaa
     9661 aattcataga caggagccgt tcctataaat ctatctaagg cagtattttc ataaggaaat
     9721 cttccttgcc aagtattggc atgataatat ccaccttcta aaagagtatc tccccaagga
     9781 tattcagatt gtcttcctcc tctagcagca tattcccact gtgcttcagt aggtaaagac
     9841 attccgctcc aattacagaa agccaatgca tcttctaaag caacatgaac tactggatgg
     9901 tcttctaaac ccactagatt actgttctca ccgtagggat gtttccagca agcgttcgga
     9961 acttgtaacc accacggagc cccagcagga tgaggatatc cctccctttc tgcctctgga
    10021 acgaacaata taaaaacaaa agaccactct tgtcgctcag ctagtgtttt atagcctgtt
    10081 tctttcacaa attgagcaaa ttcttgattc gtaactggag tatccgcaat ggaaaaatcc
    10141 tttacactaa cagcgacttg aggaccttca aaatcatcta aaaagccctc ttcagaattt
    10201 gttcccatca taaatgtccc acccttgatt tttttcattt tcatgattga ctatcccccg
    10261 ttttcgttag agttggcgtt attttagaaa gttcaacagg aactaactct tcatccacaa
    10321 caaaaccttc ttctctatat ctaagaaagt ctactaattt tttcttcatc tgacgaacaa
    10381 taggttggta tttttcgctt ggatacaaat cattcatttc atgcggatct tttttcatat
    10441 caaataactg ataatgattt aaaacaggaa accagataaa tttccactgg tctgtcaaga
    10501 tatattgact agaatctttt cctaaagcat gttcaccatg aaattctgtt cgccacccct
    10561 cgtactgtcc aaataataaa ttcttaacac tccgtccatc tagttcatct gtagtagttc
    10621 ctcctgccaa atccacgagt gaaggaaaaa tatcttgaat tttcactaat tgcttgattg
    10681 ttcccctatt accagctatc aatccagccg gatcgtaaat aaacgaagga atgtggatac
    10741 ttccctgata aggatatccc tttctaaaca gataatgttc accaagctga tctccatgat
    10801 ccgaaacaaa ccagataatc gtatctttat catgacgaaa ttcttttagg gaagtcaaaa
    10861 agcgactaat ttgatggtct atatgagtaa tcaatccaaa ataagcagca accattcttc
    10921 tttgatcatc ttcctttaat ttccctctta aagcataaat agacggaatt tgcttttcta
    10981 atacttccca attcccgata tgcaaatcga gaaattgagg caagcgttcc atatacatat
    11041 caaaatagta ttttggggga tttaatggag catgtggttt ttcaaaagac attttgagaa
    11101 aaaaaggaac cgttggatct cttctttgta aaaatgaaat ggactcactc acaacccagt
    11161 ttgttggatg taacttctca tctttatccc aaggtctagc ttcccaagaa ttacaatcca
    11221 ttccgtcatc aatcaaatca acatcataac caactttacc ctttaaaaag gctagatagt
    11281 cactaacata atcaaattga cttccataag ccttatcata ctttctatca acatgtagat
    11341 acccatcgtg aagtaagaca tgatcaaacc caagtctctg tctggaagga aaaacatgca
    11401 tcttaccaat acactctgtt tgataaccca tatctttaaa gacttttgga agagtattag
    11461 taaaattcca aggcacctca tcttgataac caacacgtcc acttttatct tggtctaagc
    11521 cagtcaataa agctgcccta gcaggaacgc aagaaggaac tggagaatag gcattttcaa
    11581 aattataacc tacactagcc atcatatcta atgttggagt gctaactagc ttatccttac
    11641 tattcaagga taaggcgtct gctctcattt gatctacaac aatcaaaata atatttggct
    11701 gttttgtctg aaccataaaa tctcctttct aatatggcaa aagaggcaca agaagatatc
    11761 tacctttact gcaccccttt ctatatcaat ctctctatat aaagcaataa cattcttgtt
    11821 atgttttata gaacaatgaa ctaaaatatg actaaatcga ttaggaaatt caaatcattt
    11881 tctagtactg ttttagaagt tacagtgtac tattccaact tcaacaaact ataaaccttg
    11941 gtctaataac aattttagtg gagataagaa atcctacacc taactcatct tacacgtaat
    12001 ctatttctat tttatcacaa aaaacgcaag taagaccatt aactcaattc agttttatct
    12061 gccattttca caaatgggaa ataagtcaag acactaataa tcaaacaaac aactgataag
    12121 atgatggcac gccaatcaaa tgctgtagag aagaaaccat ataaaattgg aggcattacc
    12181 caagtaacat tttgtgtaac aggtgaaaca agaccccagc ttgttgccca gtaagctacc
    12241 gttgccatga aaaccgggct aagtacaaat ggtataaata gcaaaggatt caagacaact
    12301 ggtaaaccat aattgaccgg ctcaccaata ttaaacagaa ctggtgctag accaagttta
    12361 gcaacttttc gataatgact gtttcttgaa aaaattaaaa tagcaagtac caatcctaat
    12421 cctccaaacc agacaaacgc cccaaaagac ccacttgtcc atatataagg aatcggttca
    12481 cctttttgga aagcatccag attcgctaac atagcaactc caaacagccc ttccatgatg
    12541 ggagccaata catttcctcc atggagacca aaaaaccaga ataacttatt taaaaagatc
    12601 atcagaataa ctgcaaagaa actttgagac aaacctagta atggcgtttg taacaccttg
    12661 taaacccaat caatcaataa gtcattgcta agtaaatgga aaacataagt caagatggct
    12721 actatataca tcgccataaa tcctggaatg atagaagtga acggcttagc aatcgcaggg
    12781 ggaactgaat ctggtaactt gattacccag ttctttttca ttactttaca gaaaataata
    12841 gaggctaaaa atccaatcat catggctgta aagtagcctc tggcattaat atggtttcct
    12901 ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag attatcaatt
    12961 ccttgtaaga tctgatctaa tttcacatct cctacatttg ccaaagggaa actctttgta
    13021 actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagc agaaactgta
    13081 tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa caaagaaaca
    13141 attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg aaaggttttc
    13201 gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt taataacaaa
    13261 gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg taaagcaact
    13321 aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg cattttatcc
    13381 aacattgtat tcatactaaa ctcctaattt ttttcaaatc gttcgtatac ttctaccact
    13441 tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc gtgtaccatc
    13501 aagaggctaa ttgatgtttc ttccccctgt gcctctcttg tcaacatttc tgtttgagcc
    13561 ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc tttagagaaa
    13621 tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc cccaccatac
    13681 ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc ctcaatctta
    13741 gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc cgtaatctgt
    13801 catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa agtcccctag
    13861 taaatagcga acttgggggc ctaaaagcaa cacatttact tcttttgttg ctacaatttc
    13921 ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat cttctgctgc
    13981 cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacacg ctaacataat
    14041 tgttacttta gccatagctt actcctttta ttttattgtc aatgttactg tttttcgatc
    14101 aactttagac tcataatcta aatgtttggg acgactagta taatgcaaat tataaactcc
    14161 tggaggcatt tgtgaaatct tttctttttc agttttattg cgaattaata aatctaccga
    14221 agtattccct ccaaagttat taaacatagg ttcattttca atagaataat ctaactcata
    14281 tccactatta gttgtaattc cttttaataa atcaggaaca tctcctctgt ttattactat
    14341 atccttagcg ccatttattt tgggagattg actataatac tcttcagctt gatgttgttg
    14401 taaatcattt ctagagtaag aaacataatc aactacaaat tcagtccctg ataaattatt
    14461 gtccactacc ccaggccaac ttcctcccat cgctaaattt aactgaacat actgaggtct
    14521 attaaaaatc tttttcgcta tattatctga tccataatat acttttctaa caatatgatt
    14581 gtcaaccatc catacaatat aatctttata ccaatcaatt gaaaaagtat gatacgaatc
    14641 attaaagttt ccactcgata atttaaaggc tgtaccacgt cctgcaaatt ttccatcatc
    14701 attatccgac tgtccataat gaagcgtttg ataaactgtc ttattcccac ttccatttga
    14761 atttcgttca ccaactaatt ccattatatc tatctcacct gttgacggcc atcctcgtcc
    14821 ctgaacagga ttaatcttcc catcaagtgt aaaatccgat ccaagagtcc agaaagcagg
    14881 aaaaactcct tgacctttcg gtaattttgc acgcatctct aatttaccat aaagaaattc
    14941 tactttccca tgtgttctaa cacttcctga attatataca acctttctgt gattgtttct
    15001 tgggttaaaa tactgatctt ccttagagcg attcgtagct ctaagaacta aatttcccga
    15061 acgtagaaaa acattctcat cacttcttac ataatgttgc tgttcccaac cacgaattga
    15121 tcctaattca tatccccaat attttttatt taattgttcg ccatcaaaat catctctcca
    15181 aattttctta tatgaatttc ctttagacag ttgatacatc tctacattat caataaaaat
    15241 attagatttt tttgtagtat cgtcttctga ccatttcact aattgaatca aaactttatc
    15301 gtgtttaccg gaattgaaag aaaacatgta ttttttccat tcggttgtat taactgtttg
    15361 atcaacagct tctccaatgt tattttgaac aatctctcct tgcttattat tatcaaacca
    15421 tcttgcggta aaatatccct ctgctccact aatatctact ttggcaaaag cagatactaa
    15481 ataatcttca ttaggttcta tatcaataac ttgtccaata tatccatctg ctctattatc
    15541 attaattgca gtagaaggaa tcatacctga gtaatgtccg ttactataac tctcatttga
    15601 attaattatt cccctatttc tagagttcca aaacagctct ccttgctcaa aatttgaatt
    15661 ttttattaaa ttttcattag caaaaacttt cacatgaata aatattaata aaaataatat
    15721 aaataaactt gaaattaatt tcttattctt cattttatca cctatttact gacctagcca
    15781 agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa atattatcga
    15841 ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact ccacaaattt
    15901 tatttctaag tgcaatttcc ttgatagtat ctgtatcaga tgtttcttgg ctagtaccaa
    15961 tagcactcag tactagccca cccttactat gaacgagatc aacgacttca cgcaactctt
    16021 ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc gcacctgctt
    16081 ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct gccccgtgca
    16141 tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca gccttaatga
    16201 tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag tcaaaaccta
    16261 actcctctat tcgattcaat gtttcaacac tagcaacacg acctgcaaca atttcctgtg
    16321 tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca attggacaag
    16381 caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa gcataaattt
    16441 taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa gctctagcaa
    16501 tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt tcagatagaa
    16561 caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc atctgtaata
    16621 tctcagatgg atttgcacta attaaacgtt tcattctcaa tcctccaata acttaaatcg
    16681 tttttcaata gctattacaa atggtaaata aagtaagata tccaaaacaa tcaatacaat
    16741 ttgtagtaat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa caggtggcat
    16801 agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg ctccccacgc
    16861 tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa tgttcaaaac
    16921 aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta taacagttat
    16981 aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat atcctaacgc
    17041 aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat ccataaatac
    17101 gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat ttgcatctag
    17161 attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt gtaatcctac
    17221 aaaccaaaaa ctactattaa ggaaaattaa gataagaaga ccaataacat tatttcctaa
    17281 aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag gtaaacttaa
    17341 tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac caggaataat
    17401 ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta tctgtatatt
    17461 atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca tagcaacaaa
    17521 taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc ctctaatgaa
    17581 aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac catcaacttt
    17641 gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa gtaatccaaa
    17701 actactatca acacccttcc ataaatagtc agcaacacca atatcaccca gatatttttc
    17761 ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca acaataaagg
    17821 tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac ccaatctagc
    17881 cgctactgga agtaactttt cttgtaataa tttttccata accttttcct cttaaatata
    17941 ttttattatc aaaagttatc aattatcttt attttttgac gtcaagttgt tatttgatta
    18001 tcttttgtta tttatatttt acaactataa gaaagccttg tcaaggattt gtgtaaacat
    18061 ttgattattt tttattatca atatataata aaaagagacc agtaagtaat gaaaagctta
    18121 aaaagattaa catctacatt tatccatagc aaaaaatcat aggattttaa gaatctgaca
    18181 actaggaaga aaactaatat gaccttatct aagaaacaat tacaactaag agctaaaata
    18241 ctggaaacag tttacacatt aggtcccatt tcacgaattg aaatcgctac taaaacagga
    18301 ataacacctg caacaactag cagtatcaca aatgatctga ttaaagaaaa tattctctta
    18361 gaacttggtg aagacgaaca cgatacaagc gtagggagaa aaaaaatcct gctagacatt
    18421 caagctaaac gattttacta cattggttgt gaactatctg aaaaacattt cacttttgct
    18481 ttaggagata atctaggaaa tatcttaaag gaagagaaag aaattgttac taaacagtta
    18541 atccaagaaa agggaaatca actcattaac cagacactaa agcaattctt aaataactgc
    18601 tctgattatg aaatagaagc aattggaatc gcgctacctg gtcgctattt agatgattat
    18661 aaaataacaa caaacaatcc tttatggcaa catatcgact tagaaatgat tcaatcccac
    18721 tttgataagc ctttgttttt ctctaacaat gttaactgta tggctatagg taaacgccta
    18781 tttagtcgcc aacagaatga ccctaatttt gcttattttc attttgctag aggtatgcac
    18841 tgttcttata tctacgacgg aaacatttat gggaaaggaa atctaatgat tggagaaata
    18901 gggcatacag ttgtttcatc tgagggagaa gaatgtagct gcgggcgcaa gggatgctta
    18961 caaacttttg ctggagaatc ttggttaatc aaaaaatcaa aaattctata tcatcaatct
    19021 ccctattctc tacttcctag tttagtcaaa aatgccgatg atattgatat ccaagttatt
    19081 ttaaccgctt atcaattagg tgacactgga ataatcacac ttatccatca agccttattc
    19141 tatcttagtc aaaccatttt aaatatcagt atgatgattg actctcaaaa aatctaccta
    19201 catagtcctt tactaacaaa tcaacacatc atccaaaaac tatatagtga aatgaactac
    19261 aaacccaaac tgctctataa tcgcctacct gaagtcatta ttgaacctta caatgatttt
    19321 acagccgcac actcagctat tgcactttgt ttgtatcata caattctaca ttcttaattt
    19381 tctattacat ttaataaata ggctaaaaag agggagtata tcataaaaag cgaataaacc
    19441 tgttttctga aaatcagaat tctgttttgt tcgctttctt tatatttgga attatactcc
    19501 tatctcccct ttttcataat gtatagtaaa atgaaataat aacaggacga atcgatcggg
    19561 acagtcaaat cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat
    19621 ctactatatt ttcgtacagg tgcttcaacc atttgaacga tttcaaatcc ttctttttgg
    19681 taaagattct gagctctttg atttgcctcg aagacattta gagaaatact atctatatct
    19741 tcattttcaa aggccaaact aacaaatttc cttaaagcct ggctacctaa gccttgctcc
    19801 tgtttctggg ggttgataaa aaatctcccg atatgaagat tgctgtcttc tagcctgatt
    19861 ttctggataa gtcccacaaa ctcttgttca tcaaagattg aaaagactcc ttccaaggct
    19921 tgaagggtca gtagaaaagg aatccttggt cccatccatt gttcttgaaa gactttgcct
    19981 aaggagttgg accattggca tacgagctga gcgttttcta tactcacctt ttcttcaaaa
    20041 cgaattgtca tcttttcctc accatcttat ctatgtttct ccattatact atttctccca
    20101 ttttttacga atagataagt atgattgatt tttatttttt tctcgtcggg agcattctcg
    20161 cttcctttct tggtttggtc attgaccgtt ttccagagca atccattatc agttcagcca
    20221 gtcactgcga ttcctgtcag actcgcttgc gtcccttaga tttgattccg attctctcac
    20281 aggtcttcaa tcgctttcgc tgtcgctact gcaaagttcg ctatcctgtc tggtatgccc
    20341 tctttgaatt agtcttagga ctcctctttc tgctttactc ttgggaattg ctttccttgg
    20401 ggcaagtcgt cctaatcacc gctggtttga ccttaggcat ctacgacttt cgccatcagg
    20461 aatatccctt actggtctgg atgactttcc acctaatcct aatagcttcc tctggctgga
    20521 atctggtcat ggtctccttc cttgctcttg gaattttggc tcattttatc gatatccgca
    20581 tgggcgcagg agatttcctc tttttagctt cttgtgctct cgtctttagc gtaacggagt
    20641 tactgatctt gattcagttc gcttctgcga cgggtattct ggcctttctc ctgcaaaaga
    20701 aaaaggaaag acttcctttc gtgcctttcc tcttacttgc tgcttgtgtg attatttttg
    20761 gtaagctact gcttgtctga taaaatccaa tttctgccat atgtccttca tgaaattatt
    20821 tcacagttaa attataaatt atttcttttg tacaaaggga tgatgttatc aaatcgatct
    20881 gttcttctat cttcttgata ctgatcaaaa aatttcattt cgactgaaaa tatttcgctt
    20941 ataaactgta aacgaatact ttgtttagac attatagtcg ctagactgac tagatgatta
    21001 ctcaaaacga cgtccagaat actctttact ttgcttggtt ttttaacaaa aatttgatca
    21061 tccaaaggtt caatcatttt gtaacctttt tgcgcaattt gacgataaaa gtaagaatgt
    21121 tgctttggag tcaataatcc taacttaaaa gctcgatact ctaaagcctg tatcgaaaca
    21181 ttcaaatccg acttcaataa aatataacta tcaggattgc tgacacgctt gccaaccctc
    21241 tcttcaaatt tgactaaaaa ctcttctttt ggcaataaaa aacatgatgc aaaataattt
    21301 gcttcttgct ccaaacgatt gccatcttca ttcatatctt tatatttgtg taaaagaata
    21361 tgtcctagct catgagctaa gtcaaaattt cgacgtacag atgatttatt cgttcccaac
    21421 acaatataag gtcttcccaa ttttgaccat gcgctataag catcagcttg gccattaatt
    21481 aatcgttcca cgatatagat gcctgaacgt tctaatttat aaagcaaatc atgattatct
    21541 tttgaaatac ctaatttttc cctggcataa agagccaatt cctcaatgga ttctccctta
    21601 tgataagatt cactcactac attacttagg tcatgaattg taatattagg tataattaca
    21661 aaactttcaa aataatcaat caaactatct accttatgta aatacatagt ttgaatatct
    21721 attgttttcc gtgttgctag gtctgcattt ctaaaggcaa ttacagaaga atcaaatcga
    21781 atgctctctt cttcctgttc aaaataagtt aaatcaacat gaaattggtt ggccaaatgc
    21841 attttggttg ataatttagg tttcgtttcg ttggactcaa actgccaaat ggcttgttcc
    21901 gttaaattaa ttctctgagc taattctgct ctacttaaac catttaacag ccgtaattct
    21961 ttcaataccc gaccattaaa catttacata ttccttacta cttttgacct tcttgttttt
    22021 ctattcttgg aataatttca aaatcttctg tttccgataa ttctgaaaaa ttaggaatat
    22081 cttgatattt agcttcttcg aaatggtacg aacttgattg aatatactca gacaaatctt
    22141 gaatcaagtg taactgacca gttcgtgcat caggcataac aacatctaca gatataatat
    22201 tgttttctga gtccgcctca taagttaaaa tcataaattt ttcgatattc gaatttttag
    22261 tagcttgttc aatttcttga atcatttcat cagaaactaa ctccatctga attggaaagg
    22321 aatgactatc ttcatcattt ttgtaggaag aatgttgatt aagataaagt gtattcatct
    22381 gagcatattc aaataagtag ccactcttat ttttttgtac caaaggaaat tggttcgtaa
    22441 gtcgcttctt accctttata attaacaata ctttcccata tttttctgta tttgtttcaa
    22501 attctaaata tccccaagtc tgtcctgcta attgtaattt atactcaaac aaatctgctg
    22561 atgcaaatgc agtatcaata tgattaggtc acgtccatgc ataaccattc gacactatca
    22621 ttgtctctct tttttctaga cgttcatcta cataatcttt ttgccctttc atcaaagtat
    22681 ctacaatttt ttgtgcctca agcgaatcaa agagatcctg attcaacata attcttcctc
    22741 ctccaaatac tttttaatga attatatcat tttcttaaag aaattactac aataattagc
    22801 tttttcttaa agttctgtgt cagagtaatt tagaaaatta tatcttctat agtaaaatca
    22861 attaaaaact gaacaaattt attgggaaat tcaaatcact ttcttaaaat attttaggaa
    22921 ccgtagtgta atattccaga ttcaattcac tataaaacta gcctttcgcc tgcaaaagaa
    22981 aaaggaaaga cttcctttcg tgcctttcct cttacttgct acttgtttaa ttatttttgg
    23041 taagctactg cttgtttgat aaagtcctga atcggctctc cttggtggag agcttttact
    23101 attttcgaac cgacgataac accatctgac accgcattga agcgttccag atcggcttga
    23161 ctagatacac caaaacctgt caagactggg atgtcggcca cttgatgaag ttgcgccaag
    23221 tgcttgtcca aatctgcacg gtaattgcct gatttccctg ttaccccatt gatggcaacg
    23281 gcatagacga atccctccgc cccttcaatc aactctttct ggcgctcaat tcctgtggtc
    23341 aagcttacta aaggaatcaa ggcgatatct gtatctgcca aaaatggttc tacaaagttg
    23401 gcatgttcat gaggcaggtc tgggataatc aagcccttca cagctgtatc agccagatct
    23461 ttgacaaagt tctccacacc gtactgaaag agggggttga agtaggtcat gatgaccagt
    23521 ggaatctctg tttcaatggt tttcaaggtt tcaactaaag cctgggtaga ggtcccgtgg
    23581 gctaaactgc gcaagccagc ttcttcgata acaggtccat ctgcaacagg gtctgaaaag
    23641 ggaataccca cttcaattgc agagacaccc aaatcttcta aaaagtgaat tgtttcagca
    23701 agaccatcca aacctttttc gtggtcacca gccatgatat agggaacaaa aattcctttt
    23761 ccagctgctt taatagcatt taattttttt gttagtgtct taggcatgag cttctccctt
    23821 ctttgctgca tctgcttcca agcggtcttt tacttgaacc acatccttgt ccccacgacc
    23881 tgataggcat acaatcatag acttttctgg tccaagttct ttggccaatt tcaccgcaaa
    23941 ggcgatagca tggctagatt ccaaagctgg gataatccct tccacacgag acaagagttg
    24001 gaatccttcc aaggcttctt cgtctgtcac agggacatag ctggcacgtt taatatcgtg
    24061 gtagtgagaa tgctctggac cgataccagg atagtccaaa cctgctgaga tagagaaggc
    24121 ttcaagaatt tgaccatggg catcttggag cacatccatg agggaaccgt gaaggacacc
    24181 tggacgaccc ttggtcaagg tagctgcgtg gtgctccgta tccacaccaa atccagccgc
    24241 ttcagctcca tacatggcta ctgactcatc ttctacaaag ggatggaaga gaccgatggc
    24301 attcgaccca ccaccaacac aggctactag ggcatctggc agattttgtc ctgtcaagtc
    24361 acggtactgt tgtttagcct ctcgaccaat gacactttgg aagtcacgaa caatttctgg
    24421 aaatggatga ggccccaagg cagaaccaag gatatagtgg gtatcatcta tattagccac
    24481 ccatgaacga agggctgcat tgaccgcatc cttgagcacg cgcgaaccat ctgtcacggc
    24541 ctcgacctta gctcccaaaa gctccatacg gaagacattg agggcttggc gtttgacatc
    24601 ttcctcaccc atgtagatgg tacattccat gttaaagagg gccgcagcag ttgcagttgc
    24661 cacaccgtgc tgaccagcac cagtttctgc gataattttc tttttaccca tgcgtttggc
    24721 aagccaaact tgtcccaagg cattgttaat cttgtgggct cctgtatggt taaggtcttc
    24781 ccgtttgaga taaatcttgg ctccgccgat atgctgggtc aagttttttg cgtaataaag
    24841 aggagtttca cgtcctacgt actggcgcaa aagctggttt aattcctctt ggaaacttgg
    24901 gtctgcctga ctttcacggt aggccttctc caactccaaa actgctgtca tcaatgtttc
    24961 tgggacaaaa cgtccgccga attttccgta aaatccatct ttatttggtt cctgatatgc
    25021 catgctttac cctctctata aatcttctaa tcttttcatg atctttttgt ccatctgtct
    25081 ccactccgct cgatacatct actgcatagg gagtaaaatg ttgaattgct tttactacat
    25141 tatcttcatt aaggccacct gcgataaaga agggctgtgc tagtccagtc gtatccagtt
    25201 gaccccaatc aaaggactga ccacttcctg ccacaggggc atcaaagagt agataatctg
    25261 cctgagaatt ggggacatgc ccatttccat ctacctgcac agcctgaata ctggcacaag
    25321 gcaaattctc aaataaatca gctgccacct gaccgtgaac ttgaaccaag tccaagccaa
    25381 ctttgtcaat cgcttccagc agttctaccc gacttggtga aacaaatact ccaacctttt
    25441 tcacatctgc aggaataagc tttgccaact cagctgcctc ttctaaagtc acctgtcttt
    25501 tactaggtgc aaagacaaaa ccgatatagt cggctcctgc tgaaacggct gtttccaccg
    25561 cttctttggt cgatagtcca caaattttaa cctttgtcaa tctgcaactc cttgattctc
    25621 tgggccacat tttctgcctg cataagagct gtccctacca aaattccgtt aaagtatggg
    25681 gctagtcgtt ccgcatcctg ccctgtgaaa atggcagatt cagaaatgta atagcgacct
    25741 tcctcaaagt aaggggctaa atctacactg gtctgcaagt cgacctcaaa ggtagtcaag
    25801 ttgcggttgt tgaccccgat aatctcagca ccaagtctgt gggctacctc tagttcagct
    25861 agattgtgag tctccactaa gacttccaga ccaagctctg tcgcgtagtc atacagttcc
    25921 ttgaggcgtt cttcggacaa ggctgccaca ataagcaaga taactgtcgc acctgtattg
    25981 cgagcgcgga tgatttgctt ttcatctatg ataaagtctt tgttgagcgt cggaatctct
    26041 acctgactgg aaatttcccg tagataatcc aaatgccctt taaagaaaac ctcatctgtc
    26101 aacaccgaaa tcatcactgc tccgttttct tcataagtct gggcctgttg cacaatatcc
    26161 acatcgagat tgatatctcc caaactaggg ctagctttct tgacctcagc gattacctgc
    26221 aagcagtcct gatgattctt caaaaattct gccaagcgat aggtctggcg cagaggctgg
    26281 atttgctcca gcttcatctg ctccacctca cgcgccttct gctctaagat tcgtgctaaa
    26341 aattcctgac tcatttttgg tactcctgta acagtctgag tttttcaagg gccttgcctc
    26401 tagcaatcac ttgacgggcc aaggcaactc cttccttgat gctatcaatc ttaccattag
    26461 catagaaacc aagaccagca ttcaagactg tcgtttccaa gaatggactt gcttcgtttt
    26521 tcagaacgct aagcaaaatt tctgcatttt cctgagcatt cccaccacga atatcttcca
    26581 tagcatagtc ttccattccc aaatcctctg gagtaaagct tgacaagctg atttcgccat
    26641 tttcaagaag tgcaatcttg gttgttccgt tcaagccagc ttcatccaac ccttctggtc
    26701 cagcaaccac gatggcacgt ttgcgaccca tatttttcaa aacctgagct gtactttcta
    26761 ggagttctgg acgactaatt ccaagaagct gtgtttctaa agccattgga tgaatcagtg
    26821 gaccagtcaa gttcataatc gttggaattc ccaattccaa acgagctggc atgatgtatt
    26881 tcatagctgg gtgcatattt ttagcgaaga gaaagacgat tccagtttta tcaaagacct
    26941 tacctagttc agctggtttg aggtcaagat tgattcccaa ggcttcgagg acatctgcgg
    27001 aaccagattt agaagaaatc gagcggttac catgcttggc catgtgaata ccgccaccag
    27061 ccaagacaaa ggctgcagtt gtggaaatat taaaactgaa agacttgtcc ccacctgtac
    27121 cacagttgtc catagcatca tgaatctcag ttggaatatg ctgggcatgt cctctcatga
    27181 cttgggcaat ggctgtacgt tcttcaggtg tttccccctt catcttaaga gctaagagga
    27241 gagaagcaat ctgcgcttca gttacacgcc cagttacgat acgctcaatg acatccgtca
    27301 tttccacacc tgataaattt tcaaattttg ctagtttttc aataatctct ttcatcctag
    27361 tttcctcact ttacaacctt ctcgataaaa ttccgaatag aagacaagcc gtctggcgtt
    27421 ccaatgctct ctggatggta ctggaagcca taaatcggta ggtttttatg ttgaatcccc
    27481 atgatggctt ggtcatcagt cgaacgagct gtcacttcaa agtcttctgg catttcctca
    27541 atcaaaatac tgtgataacg catgaccgca cgtccatcct caataccttg atacaaaaca
    27601 gatggcgctt caaagttgat attgctctgt ttcccatgca tgacttttgg agccaaacct
    27661 agcttaccac caaagacttc tgcaatggct tggtgaccca aacaaatccc tagaatcggc
    27721 ttcttgcctg caaaatcaca aatcatgtct tccatctttc cagcatcaac tggccaacca
    27781 ggaccaggag aaaagaccag accatctgct ttttcagctt cttcatacag cttggaatca
    27841 tcatttctca aaacctgaac ttctgcaaaa ttcccaatgt attgggccaa gttataggta
    27901 aaagaatcat agttgtcaat caataaaatc atggtcttag ttctccaatt ctagtcatag
    27961 attttgcttt gttaatggtt tcttggtatt cgttttgggc gatagagtcg tagacaatcc
    28021 ctgccccagc ctgcacatag gctctttgat ttttgagaat catggttcgg atggcaatgg
    28081 ccaaatccat atcacccgtc gcagacaagt agccgattgc tcctgcatat acgctccgtt
    28141 tttccgtttc cagttcatag atgcgtctca tagctcgaat ttttggtgct ccagaaacgg
    28201 ttccagcagg aagcgttgct ttcaaggcat ccatggcagt gagttctgca agcaaacgtc
    28261 ccttgaccac actggtcaaa tgcatgacgt agcggaagag ctccacctcc atatacttag
    28321 taacttggac actggccgtt tcagagatgc ggccaatatc gttacgcccc aagtctacca
    28381 acattcgatg ttctgctgtt tccttctcat cagagaggag gtcagtcgcc aaggccttgt
    28441 cttcttcatc cgtagcccct cttggtcgcg tccctgcaat cggattggtt gtcacgatgc
    28501 catttttgac agaaaccaaa ctttctggac tagctccgat gatttgataa tccccaaaat
    28561 catagaaata aaggtaatta gatggattag tcacgcggag atttctgtag aagtcaaatg
    28621 gatttccagt aacttctgct gagaaacgct ggctgagtac acattggaac atatctccgt
    28681 tacgaatcaa gtcacgagct gtttctacca ttccctcaaa cttatgtgga gcgatatgcg
    28741 gtttgaagtc aagtggtgat aaatccaagt cttcaaattc atttggagca ggaatgcgta
    28801 attcctcaag cacttggttc aaggattttt ccaaggcctc ttgactgcgc tcactataaa
    28861 gtgcatcctc tatgacatgt atcttctcct tcttgtggtc aaagaccata tagctctcat
    28921 agacaaagaa atgcatgtct ggcgtcccaa ttgtatcctc agggatttga ccaatttctt
    28981 cataaagcga aatcatatcg taaccaacaa aaccaatggc tcccccacca aaagggaggt
    29041 ctgaatggtg ctggctctta tgagtcactt cataaaggaa atccaaggga tcccgatcaa
    29101 tcgcttgacc attttgatag agaactccat tttcaaactt aatctcaaaa actggattat
    29161 aggctaggat agaaaaacga gctgtttcct tgtctctcgg aatactctct aaaataacct
    29221 tatgttgccc ctttaagcgc atataagcca agattggtga taagacatct ccatgaatga
    29281 ttcgttccat tgtcatttcc ctttcagttc taattctagt tcgtggcgac tgtatgaaaa
    29341 atccccacgc aaaataactt gcgtgaggac gaaattcgcg gtgccacctc aattatagga
    29401 tttctcctat ctctcattcc tgtctcagat atctcctgta acaggctgtg cgataaaggg
    29461 cactcccttg agaatgatgt tttcttctct cgtttcagat gaacccaact ttacagcttt
    29521 ctctgcttgt tttcagcaac cacaagctct ctgtgagaga aaagactgta atttttccat
    29581 ctattatttt ttagcttcta gtaatctgca atcgcagcta ggtccttgcc tccacgacca
    29641 gagacattga tgaagagatg ttcatctcgg tacaccttta tactcttcga aaatctcttc
    29701 aaaccgcgtc aacgtcgcct tgccgtaggt atggttactg acttcgtcag ttctatctgc
    29761 aacctcaaaa cagtgttttg agctgacttc gtcagttcta tccacaacct caaaacagtg
    29821 ttttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg agtattacta
    29881 gcttttttcg tattagtcca gcctttttgt ttacttttag taggaggcaa ggagctgtag
    29941 atagaactca agttcatcaa agcgacttaa ggccctaata aaagataaac caaacgacgg
    30001 atagaaaaaa gcccacacac agaatatact tccgtgtgag ggcgttggta acgcggtgcc
    30061 acctcaatta taaagggact atccctttac atctctgcct tgtttaacaa caagctgcac
    30121 tgtaaggtgt gcgcaccgaa ttttcattgt ttcaaattca ttttcaaaat cagcccactt
    30181 tcactacttc caaccaccta ttcacaatca ccacaggctc cctgaagatc aaaaatagtt
    30241 acttttctga tttgttgaac ttattttaat actttgtttt ttctttgtca agactttttt
    30301 acgatttttt tgatatcatt cgaatgtgac catatcttcc ttagatcgaa catgaacatg
    30361 tcccacttgt tagaaattgg atccaactca atagaaactg aatggaggct aaacagaact
    30421 tattttagaa cactccatct tttccactag gattttcaag aattaaacaa tactagaaac
    30481 tctgtctcct aacaaattta ggagaaactt caacagatgt gacactttcc cctttaataa
    30541 ttgctaaaac accttctatc atttctttag ccaatttaac ataattggga gcaattgtag
    30601 acaaagctgg agtataatac tgagaaatag gaatattatc aaatccaatg atagaaatat
    30661 catctggaat aagaattcct ttctcatagc acgcacgaat caagccctga accttttcat
    30721 ctcctgaaac aaaaataatg tccggataat tttgggtagt caagtgctgc attgcataag
    30781 aataaactga atcaattgta gataagccat aaatgacttt taaatccata aagtaatttt
    30841 tatcattcag aaaagaacgc acacctcttt cacgatcctt attaacatgg gattctcctc
    30901 ccataagcaa ccacatattt ttaaattttt cttcagttac agctttcatc atatcataag
    30961 tagcttgaaa attattatta gatacataga ctactccaga cgtttgagat tcaccgaaaa
    31021 caagaaaagg catatggttc ttctttaaat actgaattct gatatcatct acactttcat
    31081 aaaaaacaat aacaccatct actaggctac ctgtgcttga tataattgaa ttactaattg
    31141 tatcctcctc tccaaagtac tcaactatag cattaacacc aaattcttta cacgtccgta
    31201 acactttatc taacagcgta tgaaaccaaa ttaaaggaaa agagtcgatt ttttttacag
    31261 aaatcaatat atttatagct tcttttttag ttaaattttt tgcatacgca tttggaatat
    31321 acgacaattc ctctataact ttttgaatcg cttgataagt ttcttcttta acatttactc
    31381 caccattaat aactcgtgaa actgtttttg gagaaaaacc tgataaacgt gcaatatcat
    31441 aaatagttac ctttttccca tttatatttt tcatttcagt cctctattac aaacattcta
    31501 atattactat acaatattta atttttttta acaagagaat ttagtaaatt atttaagatc
    31561 cacaaattca caaaattaat tttacaaata ttcttcccct tcaaaaaagt ttaaattgca
    31621 tttcacacct ttatttttaa gaatgtttcc aacttcacga caaataaatt catatgagaa
    31681 aaaactacca taaaattgta gattaacttt ttcagtaaaa tgtgtaggat ttataaaaac
    31741 atataatagc ctgtcaatgt aacattttaa catagagtta attttttctt taaagataac
    31801 atttgttatc aactcatcag gaggtaaatg aaaggcaaac accatttcac aaatatcata
    31861 aaaagaaata aatttgtata cttgtatcaa acaattatta tcaaaatatt ctattttacc
    31921 taaatcaaaa ttgattttat aatctttcat aaaaacctct gagcaaaaat ctactcaaaa
    31981 attagatgat taaaatatct aaaaaacaaa aggacaaaaa catctgtccc tttgtttact
    32041 aaatttcagc taatttcttc gacataaata acacctacaa tattagcaat ttcttccatc
    32101 agtcgaagat gttcaaatct acctgataat tccagagtaa taaatgacgc tattttttta
    32161 tctggaacat caaagtattc aattctgtca gaattaacat ctccaaacgc tgttcttgaa
    32221 tcggtcattc tgataccatt ttctgcacaa taaaccaata cacgattata ggcttctgta
    32281 gatttaacta ctatatacaa ttcaatcatt ttagaacgat tttgtagata tttttttagt
    32341 ggttggaaca tggatatcac accccaaaca gaaatggcta ctaaaagagc tccctcataa
    32401 aaacctactc caatagctaa tccaattcct gccgaagccc aaatgcctgc tgcagttgtc
    32461 agacctgtaa ttttcttttt atctgtaata agaatcgttc cagcgcctag aaaacccaca
    32521 cctgatataa cttgagctcc taatcgtgta ggatctcctg tcccaaattt ataagatacg
    32581 tattcattcg tcatcataat caaacatgca gctagacaaa caatactata agttcggatg
    32641 cctgcaggct gggatttgct ccctctctct aaaccaatta tactaccaat gactactgat
    32701 aaaacaatcc tgacaactat ttcaatattt gataacccaa gactagtggc tgtcatgatt
    32761 atttccttac tttacgcccc ggtctttgtg tgaagtataa taccgttcca gaaataatca
    32821 tcagaacaat tgtataaaca aataccagag cttgtgcatt agatgttgct gtttcatcac
    32881 ctgcagatcg aatcgtaata cctaatggtt gagctagggg gtggtaaagg aatacagata
    32941 agtcgaagtc agttaataaa gagttaaagt ttaaagcaat aacagagaga acaaccggta
    33001 aaataaatgg aatgataacc ttcatcatag tataaaaagg tgaagcaccc atacttcttg
    33061 ctgcatcttc catctcatca tcaacactaa ataaaatagc acgtaccatt ctataagaaa
    33121 atgggatttt tacaactata tatgcaataa gtagaattac caaactacct accaaaatct
    33181 gattcaagac aagaaattgt ggctgattaa aagtaaataa taaacttact gctaaaagtg
    33241 tacttggtag taaccaagga agtagagcac catattcaaa taagaaatca aaacgagatt
    33301 tatgttttct gacaacacga gcaaatacaa ctgcgagaat tgttgctgtt gtcgcagcaa
    33361 taatagaata aataaagctg accaagaatg gagagaatgc cgcactatta ctaaagaata
    33421 agcgataatt ttctaaagta aagtttgata atgttaagtt acctgtttga attgcaactg
    33481 gatctgtaaa tgagtataat actataaaaa ttagtggaag catgaaaact gtgaacaatc
    33541 catatgctac aatgtgagca atgatattcc aaggcttaga cgcaattttt tgttttttaa
    33601 gaggcgcttt agtcttagag atagaaatat aatttccacc tttttctatc ttattcatga
    33661 tagtaagcaa aattgtagtt gcaataccta aaataattgc aagtaaggca gctaaatcac
    33721 gagaattccc catccctgca aatgtaataa tcattggatt tatagtttga aattctttac
    33781 caccaacaat catgggtgct gctactgcag ataaaccact aagaaaaacc ataatagtaa
    33841 gtgcaaatag agttggaatt aaggttggta acactacttt tcggaaaaca gtaaatggtt
    33901 ttgctcccat atttcgagca gcctcaatag tgtgatagtc aacgcttcga attgtatttg
    33961 ttaaaaacaa tgtatgatta gcagttcctg aaaatgtcat aatgaataag actgcaccat
    34021 acccaataaa ccagttagag tctaaagaag ggataacatt ttgtaaaaat tttgtaatca
    34081 atccataagg accatagaca aatttatatc cagtcgctaa aaccactcct ccataaatta
    34141 aagaggtcat ataacctaat tttaaaattt tagcaccttt aatatcaaag tactctgtaa
    34201 atagaacaca aagaatacct acgacattaa ctgtaataat gagtgaaaat gctaacttaa
    34261 aactgttcat aatactctga agtgccctct gagattttag aacacgatgt acagcatcaa
    34321 gggaaaattc tcctcctttt acaaatacat tcactactag atcaaagttt ggataaataa
    34381 taaatgttac taagaaccag attaacccta aacgaataag ccaatctttt aaatttaatt
    34441 tatgacgcat actgcacctc cttaaaattg cagaacgtct gatggtgtga taaataattc
    34501 cacactttct ccgacagatc taatagcagc ctgactatca atacttgtta cattaagaat
    34561 ctgactttca gaaactttta ttgtatagtg aattgtaact ccagaaaact caacatcaat
    34621 aattgtccct tttagaataa aatcttgttc agtttcacga ttgaatcgaa ctttctctaa
    34681 tcgaatgtaa ccttttttat cctctaagaa aacgcttgta tttttcagta atacttcgtg
    34741 gactgtttca tcggtcaaaa cattaatatc tccaataaaa tcacatacaa attcagtttg
    34801 agaattatga taaatctcta ctggtgtacc gacctgttcg atgtatccat tgttaaagac
    34861 tgcaattcta tcagataaag tcaaggcttc ctcttgatca tgagtaacat ataaagtagt
    34921 aatacctaac tctttttgaa gtcttttcaa ctcttttctc aaatctacac gtaattttgc
    34981 gtcaaggttt gacaatggtt catctagaca aagaatttta ggttcaagaa ccagagcacg
    35041 agccaatgct accctttgtt gttgaccccc agataattct gatacattac gctgtaactg
    35101 ttgatcagag atcttaattt ttgctgccac tgctgatact ttagctttaa taacatctgg
    35161 agctaccttc ttaactttta aaccaaatgc aatattatca aaaacagtca tagttggaaa
    35221 tagcgcataa gattgaaata caataccaat tccacgcttt tcaggttcca aatgagtgac
    35281 atctgttcca ttaacttcaa tacttcctga tgatggatct aaaaaaccta ccaatgctct
    35341 caaagtagtt gatttaccac atcctgaagg cccaagaaat gtaaagaatt ccccttcatg
    35401 tatatctaaa ttcagattat caattgcaac aaaatcacca tatttaattt gaatattatc
    35461 aaatttaatc atctcactaa ctccctctat tactaaaacc aaaagcctct ctttatttct
    35521 tccttaaatt tagaaataat agagagactt ggacataaaa aataactctt atttcttatt
    35581 ttacgtattc taattcagct ttttctaccc attcatccaa atgctttcca acagcttccc
    35641 agtcaatatt ttgtggtttc acttgatcaa caaatttttt cgtatcttca ggtaactctt
    35701 ttagggcatc tttattggca ggaatagatc caaatttttt actatattct acttgaattt
    35761 ctgattgacc aaaccaatca ataaattctt tagctaacgc ttgtttttta ctagtgctta
    35821 aaaccatagt ttgttcagtt acaaatggta caccaatctc aggagtcata actttgaatt
    35881 caacattttg ttctttttgt ccaattaacg ctccagaacc ccagatcata ccgtattgaa
    35941 ttgggctttc tttatctaac gctttaacaa ctgagctttc acctttttga agagtatagg
    36001 catttgcaaa gtattctttt gcaacttccc atcctttttc tgatacgcct aaatctcctt
    36061 tttcatcaag gtaacgaact aagatacttg ctagaattgc ccgtcctgta ccaccttgaa
    36121 gaccataaat tgaatattta cctttatact tactacctaa ttcagtccaa tctttaggca
    36181 tttcttttac atcaggcgcc ccaattaaaa ctaatggttg aacaatcaca ggattataat
    36241 aattatcttt atctgataaa gattgatcaa ttttatctaa ccatttaggc ttgtactgta
    36301 ctagtaattt ttgatctcta attttatttg agtcaacagc accaattcca aataccatat
    36361 ctgcaactgc attattcttc tcagcaataa cacggtctgc taattgagcg ccagcgatat
    36421 caaccatttt tatattaaaa ccagcttctt ttgctttagc agttaaccaa tcaccacgac
    36481 catttgagac tgagttcgaa tagataacta attcttgact tttatcagct ttttcttcag
    36541 atgaagaagc agtcgtagaa tttgaacctc cagagcaagc agcaagtgta gtaagagcaa
    36601 ctcccgttgc aagtacagta gaccaaactt tcattttttt catgataagt tctccttttt
    36661 tattatttta tttaaatttt tcgtgatatg gaacaaattg cctcatatct tcaaatacag
    36721 tatagtcaat acggtttaca gtaatagttg gaatcttctc taataaaatt tcagttaatt
    36781 ctgctctgac tttagtaaac tcttcttcct cctcttcggt tagaggaatc cgaagatacc
    36841 caattgaaat atggaattga tatctatcat gattagggaa acaaacacct gctttttctg
    36901 agacataagt acgaatttct tctaatctct ttgcagaagc ttcatctgca ggttcaacta
    36961 gtatgttttg ttttcccatt tcagttatac gcatatgaat ttcttcatcc aacaatggaa
    37021 aaatttcaag ttgtttagca aagtaatcat gtatttcctg taaaggtgta tctagaggaa
    37081 gattactgct ccaaaactcc gtttcacgat tttcatggca caacaattca attacagtca
    37141 tgtgaataga attccttgga gttaaagtaa acttatcgat aaatggtaat tctctataac
    37201 gtgattgaat aatatcaaca acttccatca aatcttgttt agtataaaga tttgctacaa
    37261 ctgtattccc agggaaatga ttaaattccc cattttcttt gaacttaatt tttgttaagt
    37321 ttttcatata aagaccttct tttttatatt tgacatcggt gtcattgtgt ttacattata
    37381 caatgacacc gtttcctttg tcaagcgctg tcaaacattt tttttaattt atctttcatt
    37441 aaaag
//
LOCUS       DAVBCR010000012        37215 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCR010000012 DAVBCR010000000
VERSION     DAVBCR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.012"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            175..1938
                     /gene="aspS"
                     /locus_tag="VQ508_000550"
     CDS             175..1938
                     /gene="aspS"
                     /locus_tag="VQ508_000550"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2489598.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGTVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKEAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVFFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETVHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1916..2857
                     /locus_tag="VQ508_000551"
     CDS             1916..2857
                     /locus_tag="VQ508_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2489599.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(2977..3057)
                     /locus_tag="VQ508_000552"
                     /pseudo
     CDS             complement(2977..3057)
                     /locus_tag="VQ508_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3193..4179)
                     /locus_tag="VQ508_000553"
     CDS             complement(3193..4179)
                     /locus_tag="VQ508_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2489600.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4189..4989)
                     /locus_tag="VQ508_000554"
     CDS             complement(4189..4989)
                     /locus_tag="VQ508_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2489601.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5242..6084)
                     /locus_tag="VQ508_000555"
     CDS             complement(5242..6084)
                     /locus_tag="VQ508_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2489602.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6086..7378)
                     /locus_tag="VQ508_000556"
     CDS             complement(6086..7378)
                     /locus_tag="VQ508_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2489603.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7489..8760)
                     /locus_tag="VQ508_000557"
     CDS             complement(7489..8760)
                     /locus_tag="VQ508_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2489604.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9310..10827
                     /gene="malQ"
                     /locus_tag="VQ508_000558"
     CDS             9310..10827
                     /gene="malQ"
                     /locus_tag="VQ508_000558"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2489605.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10853..13111
                     /gene="glgP"
                     /locus_tag="VQ508_000559"
     CDS             10853..13111
                     /gene="glgP"
                     /locus_tag="VQ508_000559"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET2489606.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLKSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14156..14728)
                     /locus_tag="VQ508_000560"
     CDS             complement(14156..14728)
                     /locus_tag="VQ508_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489607.1"
                     /translation="MDKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHVAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15350..16198)
                     /locus_tag="VQ508_000561"
     CDS             complement(15350..16198)
                     /locus_tag="VQ508_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2489608.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16350..16628
                     /locus_tag="VQ508_000562"
     CDS             16350..16628
                     /locus_tag="VQ508_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489609.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16630..18693
                     /locus_tag="VQ508_000563"
     CDS             16630..18693
                     /locus_tag="VQ508_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2489610.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIEFIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18771..20027)
                     /locus_tag="VQ508_000564"
     CDS             complement(18771..20027)
                     /locus_tag="VQ508_000564"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2489611.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKVFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20169..22634
                     /locus_tag="VQ508_000565"
     CDS             20169..22634
                     /locus_tag="VQ508_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2489612.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVAGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNLTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGALATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22699..23610
                     /locus_tag="VQ508_000566"
     CDS             22699..23610
                     /locus_tag="VQ508_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2489613.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23840..24538
                     /gene="dapD"
                     /locus_tag="VQ508_000567"
     CDS             23840..24538
                     /gene="dapD"
                     /locus_tag="VQ508_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2489614.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24606..25736
                     /locus_tag="VQ508_000568"
     CDS             24606..25736
                     /locus_tag="VQ508_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2489615.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25748..26287
                     /locus_tag="VQ508_000569"
     CDS             25748..26287
                     /locus_tag="VQ508_000569"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2489616.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26271..26948
                     /locus_tag="VQ508_000570"
     CDS             26271..26948
                     /locus_tag="VQ508_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2489617.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQ508_000571"
     CDS             complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQ508_000571"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2489618.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27941..28957)
                     /locus_tag="VQ508_000572"
     CDS             complement(27941..28957)
                     /locus_tag="VQ508_000572"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2489619.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29169..29548
                     /locus_tag="VQ508_000573"
                     /pseudo
     CDS             29169..29548
                     /locus_tag="VQ508_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29728..30153)
                     /locus_tag="VQ508_000574"
     CDS             complement(29728..30153)
                     /locus_tag="VQ508_000574"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2489620.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(30221..30871)
                     /gene="phoU"
                     /locus_tag="VQ508_000575"
     CDS             complement(30221..30871)
                     /gene="phoU"
                     /locus_tag="VQ508_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2489621.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30886..31638)
                     /gene="pstB"
                     /locus_tag="VQ508_000576"
     CDS             complement(30886..31638)
                     /gene="pstB"
                     /locus_tag="VQ508_000576"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2489622.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31640..32455)
                     /gene="pstA"
                     /locus_tag="VQ508_000577"
     CDS             complement(31640..32455)
                     /gene="pstA"
                     /locus_tag="VQ508_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2489623.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32448..33311)
                     /gene="pstC"
                     /locus_tag="VQ508_000578"
     CDS             complement(32448..33311)
                     /gene="pstC"
                     /locus_tag="VQ508_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2489624.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33429..34304)
                     /locus_tag="VQ508_000579"
     CDS             complement(33429..34304)
                     /locus_tag="VQ508_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2489625.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34471..35802)
                     /locus_tag="VQ508_000580"
     CDS             complement(34471..35802)
                     /locus_tag="VQ508_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2489626.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35803..36510)
                     /locus_tag="VQ508_000581"
     CDS             complement(35803..36510)
                     /locus_tag="VQ508_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2489627.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36594..37079)
                     /locus_tag="VQ508_000582"
     CDS             complement(36594..37079)
                     /locus_tag="VQ508_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2489628.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37065..37215
                     /locus_tag="VQ508_000583"
                     /pseudo
     CDS             37065..37215
                     /locus_tag="VQ508_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10895 a         7058 c         8035 g        11227 t
ORIGIN      
        1 atacctacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagcgct
       61 aatttttagg gctttcacct gacttttaaa gactggcaaa cttttctgat gattttcatg
      121 aagagcctgc gcttttatgg taaaatagta acagaataaa agaggagaga aataatgaaa
      181 cgtagtatgt atgctggtcg tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa
      241 ggatgggttg gccgtcgtcg tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt
      301 gaaggaatca tgcagttggt tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca
      361 gctgaaagcc ttcgtagcga atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag
      421 caagccaatg ataagttgcc aactggtacg gttgagttaa acgtgacagc tcttattgtg
      481 cttaacacag ctaagacaac accatttgag attaaggatg gcattgaggc aaatgacgat
      541 acacgtttgc gttaccgtta ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa
      601 cttcgtgcca aggtgaccca ctctatccgc aactacttgg atgagttgga gtttatcgac
      661 gtggagacac cattcctttc taagtcaacg cctgaagggg cgcgtgatta tttagtgccg
      721 tctcgtgtta ataaggggca tttttacgct cttcctcaaa gtccacaaat cacgaaacag
      781 ctcttgatga atgctggttt tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag
      841 gacttgcgtg gagaccgcca gcctgaattt actcaggtcg acttggaaac gtccttcctt
      901 actgagcaag aaatccaaga tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca
      961 aaaggcatcg aagtaacgct accattccct cgtatgaaat acgatgatgc tatggctctt
     1021 tacggttctg acaagccaga tactcgtttt gacatgttgc ttcaggactt gacagaagtg
     1081 gtcaaaggtg tagactttaa agtcttttca gaagcacctg ctgtaaaagc gattgtggtc
     1141 aaagaagctg cggacaacta ttcacgtaaa gacatcgaca agatgacgga agtagccaaa
     1201 cagtatggtg ccaaaggtct tgcttgggtc aaggtggttg atggagaatt aaacggacca
     1261 gttgccaagt tcttgactgg tatccaagaa gaattgacaa cagcgcttgc tcttgaagat
     1321 aaggacttgg ttttctttgt ggcggatacg cttgaagtgg ctaatgcaac actgggtgcc
     1381 cttcgtggac gtattgctaa agagcttggc ttgattgata atgataagtt caacttcctt
     1441 tgggtggttg actggccgat gtttgaatgg tctgaagaag aaggccgcta catgagcgcc
     1501 caccatcctt tcacccttcc acaggaagag actgttcacg aattagaagg tgatttggct
     1561 aaggttcgtg ccattgctta cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc
     1621 cttcgtatca accaaaaaga ccttcaagaa cgcatgttca aggctcttgg tttctcaact
     1681 gaagaagcca atgaccagtt tggtttcctt cttgaagcca tggactatgg tttcccacca
     1741 cacggtggtt tggctatcgg gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat
     1801 atccgtgaag tcattgcctt tcctaagaac aacaaggcaa ctgacccaat gacacaagct
     1861 ccatcaacag tcgctctcaa acaactagag gaactcagct tacaagtaga agaagatgaa
     1921 acaaacaaaa cgaattaagc ggtggcgcta ttatctgcgc cgctttgctt atcagataaa
     1981 aattttacgt gtcttacaaa gtatctctcg agaaaagtat gatgagaaga tttcggcctc
     2041 tctggtctat ggttttttat cagcagtagc agttaatttc tttttccaac cagggcatgt
     2101 gtattcgagt ggtgcaacag gtctggcaca gattatctct gccttgagta atcactggtt
     2161 tggttttcat attccgattt cgctaagctt ctacgccatt aacttccctt tgatggtctt
     2221 agcttggtat cagattggcc ataagttcac cgtctttacc tttatcacgg tatctatgag
     2281 ttccttcttt atccagtttg tccctgtggc aaccttgacg gaggatccca ttatcaattc
     2341 cctttttggg ggtgttgtta tgggtttggg gattggtttt gctcttcgaa acaatatctc
     2401 cagtggtggg acggatatcg tcagcctgac tattcgtaag aaaacgggta agaatgtcgg
     2461 tagtatttct ttcttggtaa atggaactat catgctgata gcaggtttga cctttggttg
     2521 gaaatacgct ctttattcta tgattaccat ctttgtctct agccgtgtga cagacgcagt
     2581 ctttactaag caaaagcgta tgcaggccat gattgtgaca aatcatccag agaaggtaat
     2641 tgaaaaaatc cataaaaaat tgcaccgcgg agcaaccatg atccacgatg cagaaggaac
     2701 ctataatcac gagagaaagg cagttttaat cactgtcatt acacgtgcag agtttaatga
     2761 atttaaacag attatgacac aagtggatcc aagctccttt gtctctgtct cggaaaatgt
     2821 tcatattcta ggaagatttg ttgagataga taattagtga ttaatactct tcgaaaatct
     2881 cttcaaacca cgtcagcttc accttgccgt atatatgtta ctgacttcgt cagttctatc
     2941 tgcaacctca aaaccatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc
     3001 atgttttgag caacctgcgg ctagcttcct agtttgcact ttgattttca ttgagtataa
     3061 aaagatcttg ggaaaagact caatttcagt agaaaaggaa gtaaatcttc ccacattaaa
     3121 acgcatagta tcaagttttt tcaatacctg atagtatgcg tttttgcttt taacgacttt
     3181 ttgcccagtc tcttattttt cgataatttt gtgggcgatc aattgacggt aacaaatttg
     3241 tttattgttt ttattatcat taataatctg gagaatagtt tcaagggaaa cacgaccaag
     3301 ctctaaacta ttgatatcga cataggctgc caagttgagc ttgggattaa ccgagtcaaa
     3361 gctgagaaca gggacatcca gctggtgttt ggcaatatag ttacaaacgc cttcagctag
     3421 gaggctatcg gttgtgatga tagcatcaat ttgtggatcg tgcttgaata atcgcttgct
     3481 aaatttatag cccttttctt ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt
     3541 ggtaagtttg taatgtttaa gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa
     3601 gagcttttta cttcctccga taaaggcaat gcgtttgcag ccttttttga tgaaatattc
     3661 agtcgcatca aaaccagctt gaacattgtc gttgtcgaca agtgggatga aaggagatag
     3721 agatttacct aagataagga aggggaactg ttcttctgcg acgagtttta cgagagggtc
     3781 ttcttcttgg gcatagagaa agattagccc atctacacgc ttgccgtaga ccatttgtga
     3841 aatagcgttg agacgctcct tctcatcttt ccctgttgct atctgaatgg catagtggtt
     3901 ttcagatgcg acttgagaga tgccacgtag aaccgatgga aagaaaggat tctggtagaa
     3961 ggcgtctgag tcatcaggaa gaactaatcc gataacctga gtatagctgc ttaccaagct
     4021 acgagcgttg aggtttgggt ggtagttgag ttccttcata gctttgcgaa cgcgtttttt
     4081 tgtttcgtcg ctaatggttg atttattttg aataacacgg gttacggttg aaggcgaaac
     4141 accagcagcc ttggccacgt ctttaatcgt aacgggcata aaaatctcct atttatggta
     4201 atttggatca cggaaatgtg ttttataaaa gatagtgacc agatacagaa caaaaagaat
     4261 attttgtaca gtaatcaggg ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat
     4321 cagagtcggc aatcctaaac agttcaagat aaagtgatag cactctttaa aggttctaaa
     4381 tgaaaagagg cgtgatttct tagtgatata aaggagaaga ctagctccta gagagacgat
     4441 aaagaaattc aaaccaaaga ggaagctcgc accgagaact aggaagaggc tgatatagat
     4501 acgattttgt tggtaccagt ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa
     4561 actctcagtc tgaatggctc ggtaagagat gcgggtcagt tccttgcttt ccttgctgat
     4621 gactagttca tttgtatcga aatgcagttg caagtcctta ggtaattcct tgatttgact
     4681 tggaccaatc acaacagaag gggcttgact agctgttcca gtataagtta atgtgccatc
     4741 gacaattgta gcatgttcag agagatcctg gacaacctca tttgtcagag gttcatagac
     4801 attatcgata aaagtttcta gcggataggt ctcctgggag ctgttttgga tagcaatggg
     4861 taccatagac aagctgataa ggaagatact ggtaaagaga agttgaaacc agttgagctc
     4921 gaaacgtttg gacaggggct tacgaaaccc ccaaatactt gaaaaatagg aaaatggata
     4981 tggaagcata ggtatctttc taaaaggtgt tttctatatg agtattatta tatagagaaa
     5041 tgcgctttat ttcaagtata gggggtaaat tgcttgctac aaggatatta tttacagtga
     5101 caattgctaa taatcaatga aaatcaaagt gcaaactagc cacaggttgc tcaaagcaca
     5161 actttgaggt tggagataaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg
     5221 aaaaagggtg gcggggataa attatccctt gtcgccacca cttgtaagtc ctgaaacaaa
     5281 gttcttttgt aggaagaaga agagaataca gattggaagg gcgatgagga tagcacctgc
     5341 tgagaagtag gcaatcttca agtttttcgc attgttaacg aaggtttgga gacctacggc
     5401 aacagtaaag tattctttct cacgaagcaa gaaactagag aggatgtagt ccccgaaagg
     5461 tcccatgaag gcccagagag cttgtacggc aaccattggg cgaacaagtg gtagaacaat
     5521 ttgccagaag cggcggaagt gtcctgcacc gtctagtttt gcagattcgt ctaaagacat
     5581 tggcactgta tcgaagtagc ctttcatgag ccaagcattc atcgggatac caccaccaac
     5641 gtagaggaag atgaggaacc agttgtggtt aagggcgttc aacataagcg ccataacgaa
     5701 gaaggctgtc aaagcggcca tagttggcac catttggatg atcaagaaga agaccaaact
     5761 ttgtttacga gccaagaagt tgtaacggct gtaggcataa ccagcaagta cgatgatact
     5821 tgtttgaaca gccatggtaa ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta
     5881 caaggtttca gtgaagaggc ctttaaagtt atcaaaattg aggtcgatat tagtatctag
     5941 tttaaaggct gagacgttac ctgctttaaa ggctgacata atggtaatca acagtggata
     6001 gataattaca attgatagac caatcaggta aaggtaagta aggctttgag tcagtctacg
     6061 tttgagttta attgagttat tcatcttaga cgtcctccat atcaaatgcg tgtagtttct
     6121 tgaatgcgat catagagatt gagatgacaa tgatagagat aatcaaggta acagctgccg
     6181 ccattgagta ttgaggagat gtacctgttg tcaaacggta gatccatgag atcaagatat
     6241 cggttgaacc agctccacct ccgacactac caggtcctcc accattgaag aggtacatga
     6301 tagagaagtt gttaaagttg aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca
     6361 aaatcattgg gaaagtgatg ttgcggaatt tttgccaagc gttggcaccg tcaatataag
     6421 ctgcttcgta aaggtcgtta ggaatagatt gcaagatacc caaagtcaaa acgtagatgt
     6481 atgggaatcc gagccaacct tgcatcataa tcaaggcaat cttagtccaa gttgggtctg
     6541 ttttccaagg aataagagct ccatcaagga aaggaaggaa tttagccaag attggcaata
     6601 cttgagtgtt gatagcaccg acactatcgt taaacatgtt tgagaatgtc aagatagtga
     6661 tgaaggctgg gacagcccaa ggaagaagga aaataacacc aaagatacgt tttcctttga
     6721 taaatggttg gttggcaatg atagctgtga agataccaat tacgatttgt aaagtagaag
     6781 ctgccaaagc ccaaatgata gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac
     6841 tcaagctcca aatgtttgta aagttggtca aaccaaccca gtccaacaac ttgtttggtg
     6901 gcaagtgttg gaagtcgtag ttggtaaagg cgatcatcaa ggttacgata actgggaaga
     6961 taatcgcgaa ggtcatggca acataagatg gaatgatcaa gaggtaaggg aagccatttt
     7021 cataaatccc tttgatcatg tctttgagtg tgcgtggaac tggaattcca ttgttaatgc
     7081 gttttgcaat cgtatgtgca tctttgatat ttgagaaata aaagagtaca taaacgatta
     7141 caaagattag atggaaggca ccacgaatca gcataaagag ggaattatca cgacctggtt
     7201 tgtcaccaag agtgatgagg ttgctcaatt caggggttgc aagtgctagg aagtaaagga
     7261 caaatacgat ggttacacca aggaagataa aacctttggc tttttgttta ttgtaaatct
     7321 gtcctaaccc aggaatgata gacagcaggg ctgctttact aggttgttgc ttttccatat
     7381 taactccttt cataggatac tactttttta taagaaagct aaattatttg tgtctctatt
     7441 gataaattca aagggggatt tgatttccac cccccttgaa caaatttttt attcaccaaa
     7501 tttttgtttg attgtttctt tgatcaatgt tacagcatcg ttagcagctg ttttagcatc
     7561 tttttgacca cttacagcat caaagagcat atttttcgct ggatcccaaa ctgcagacat
     7621 ttgagagatg tttggcagtg gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa
     7681 ctcatcgttt ttaccttcag cgtatgaacg agcctcagta ttagctggga tttcgttagt
     7741 cttatcatat aatacttttt gttgttcagt tgcaacaagg aagtctacaa atttttgaga
     7801 agcttcaagg ttcttaacgg cttgaggaat gacccaagct ttaccaccac cgaatgcagc
     7861 atattctttt ccatttggaa gagttgggat agttgcaact ccgtagttta ctttagcatc
     7921 tttaaaggct tgagctttcc aaggtccgtc gatgatagca gctgttttac cttcttggaa
     7981 ttgagtttgg attaagtttc cagcaccttc tgtatcttgc atacctttag gccatttttc
     8041 gtaccaagat ttagcgtagt tgatacctgc gatagaaccg tcgtttgcaa gaccgatgtc
     8101 tttagcgtct ttaccgtttt ggccaaagac gtaagcaccg ttaccggcaa gaagtccata
     8161 tgtatagtag aagtttgtcc agtcagctag gaaggcagta gttttaccat cttcaccagc
     8221 gaatgcgtat ttgctatctt tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc
     8281 tttcaccaag tctttgttgt agtacataac aagtgactcg ataacggcag gagcaccgta
     8341 aactttacca ttagcagctg ttacaagaga tttagttgtg tcgtctgttt tagcaccatc
     8401 gctcaatttc acttctgaaa gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg
     8461 agccatcata acatcaggga cattaccaga ttggttgtca agagaaagtt tatcaagacc
     8521 tcctagagca tcaccagttt taagagtgac ttttactcca gcttcttttt cataagcttt
     8581 agcaacctct tcaatatagc tcttatatcc ctcgtctaca tatacagtga gttctttgac
     8641 ttcagatgaa ccagaatcag caggcttatc agcagttttg cttccgcaag ctaccaaaag
     8701 caagctagca agtgtagcag ttccaagcac cgcagtgctc ttcataaatt tagatgacat
     8761 agtgtattcc tcccaaagaa tagcaagttt tattgataag gaaacgcaaa cgttttcctt
     8821 tatgagctta gtatagcaca aatagaaaac ggttgcaagt attttttgta aaaattttaa
     8881 aaaattttta agcttgattt gatagcataa ttttgcattt taatagaaga aaagtatgaa
     8941 atagataaga atacagtatt tttaataaaa atatatggaa tctgtctaaa gagggaaatc
     9001 aggaggggct acctccctgt ttctaatccc tatctatttt ctatagacgt ttgtgctttg
     9061 ttgcatataa attcaagctc cctaaggagg ttttgtactt tgtagactgt ctcgtgagag
     9121 tcaaacttta ggattagata gttttaagtt atctttgcta aaatgagtta gtctccttaa
     9181 aaataatcaa aaaagttttt taaaaacgct tgcaattatg cttgaaaagg agtatactta
     9241 taagtaacgc aaacgtttgc gtctgcaaaa tacgcaacgt tccattattt taacacacga
     9301 ggtgctatta tgaaaaaacg tcaaagtggt gtgttgatgc acatctcttc tcttccagga
     9361 gcttacggaa tcggatcatt tggtcaaagt gcttacgact tcgttgattt cttggtccgt
     9421 acaaaacaac gttactggca aatccttcca ttaggagcaa ctagttacgg ggattctcct
     9481 taccaatctt tctcagcctt cgcaggaaac actcatttta tcgatttaga tatcttggtg
     9541 gagcaaggtt tgttgggagc gagtgacctt gaaggagttg actttggtag cgatgcgtct
     9601 gaagttgact atgctaaaat ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa
     9661 cgtttctttg aagtcggaga tgttaaagat tttgagaaat ttgctcaaga caaccaatca
     9721 tggcttgagc tctttgctga gtatatggct atcaaagagc attttgacaa tcttgcttgg
     9781 actgaatggc cagatgcaga tgctcgtgct cgtaaagctt cagcacttga aagctatcgt
     9841 gagcaattgg cagacaagtt ggtttaccac cgtgtgactc aatacttctt cttccaacaa
     9901 tggttgaaat tgaaagctta cgctaacgac aaccacattg aaatcgttgg ggacatgcca
     9961 atctacgtag cggaagattc aagtgatatg tgggcaaatc cacatctctt caaaacagat
    10021 gtcaatggta aggctacttg tatcgcagga tgcccaccag atgagttttc tgtaactggt
    10081 cagctttggg gtaacccaat ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg
    10141 tggattgaac gcttgcgtga aagcttcaaa atctacgata tcgttcgtat cgaccacttc
    10201 cgtggcttcg aatcttattg ggaaatccct gctggttccg atacagcagc acctggtgag
    10261 tgggtgaaag gtccaggcta caagcttttt gcagccgtta aggaagaact tggtgagcta
    10321 aacatcatcg cagaagacct tggcttcatg acagatgaag tgatcgaatt acgtgaacgt
    10381 actggcttcc caggaatgaa gattcttcaa tttgccttca acccagaaga cgaaagcatt
    10441 gatagcccac acttggcacc tgctaactca gttatgtaca caggaacaca cgataacaat
    10501 acggttcttg gttggtaccg taatgagatt gatgatgcga ctcgtgagta catggctcgt
    10561 tacacgaacc gtaaagaata cgaaacagtg gtacacgcta tgcttcgtac agtattttca
    10621 tcagttagct ttatggcaat tgcaactatg caagatttac tagaattgga tgaggcagct
    10681 cgtatgaact tcccatctac ccttggtgga aactggtctt ggcgtatgac tgaagatcaa
    10741 ttgacaccag ctgtcgagga aggtttgctt gacttgacaa caatttatcg ccgaattaat
    10801 gaaaatttgg tagatttaaa gaaataagac aataatcagg agacaactaa acatgttatc
    10861 actacaagaa tttgtacaaa atcgttacaa taaaaccatt gcagaatgta gcaatgaaga
    10921 gctttacctt gctcttctta actacagcaa gcttgcaagc agccaaaaac cagtcaacac
    10981 tggtaagaaa aaagtttact acatctcagc tgagttcttg attggtaaac tcttgtcaaa
    11041 caacttgatt aaccttggtc tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa
    11101 agacttgatc gaagttgaag aagttgaatt ggaaccatct cttggtaatg gtggtttggg
    11161 acgtttggct gcctgcttta tcgactcaat tgctactctt ggtttgaatg gtgacggtgt
    11221 tggtcttaac taccactttg gtcttttcca acaagttctt aaaaacaacc aacaagaaac
    11281 aattccaaat gcatggttga cagagcaaaa ctggttggtt cgctcaagcc gtagctacca
    11341 agtaccattt gcagacttta ctttgaaatc aactctttac gatattgatg ttactggtta
    11401 tgaaacagcg actaaaaacc gcttgcgttt gtttgacttg gattcagttg attcttctat
    11461 tattaaagat ggtatcaact ttgacaagac agatatcgct cgcaacttga ctctcttcct
    11521 ttacccagat gatagtgacc gtcaaggtga attgctccgt atcttccaac aatacttcat
    11581 ggtttcaaac ggtgcgcaat tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca
    11641 tgaccttgct gactacgcag ttgtccaaat caacgatact cacccatcaa tggtgattcc
    11701 tgaattgatt cgtcttttga ctgcacgtgg tatcgagctt gacgaagcaa tctcaattgt
    11761 tcgtagcatg actgcctaca ctaaccacac aatccttgct gaggcgcttg aaaaatggcc
    11821 tcttgaattc ttgcaagaag tggttcctca cttggtacca atcatcgaag aattggaccg
    11881 tcgtgtgaag gcagagtaca aagatccagc tgttcaaatc atcgatgaga gcggacgtgt
    11941 tcacatggct cacatggata tccactacgg atacagtgtt aacggggttg cagcacttca
    12001 tactgaaatc ttgaaaaatt ctgagttgaa agccttctac gacctttacc cagaaaagtt
    12061 caacaacaaa acaaacggta tcactttccg tcgttggctt atgcatgcta acccaagatt
    12121 gtctcactac ttggatgaga ttcttggaga tggttggcac catgaagcag atgagcttga
    12181 aaaactgttg tcttatgaag acaaagcagc tgtcaaagaa aaattggaaa gcatcaaggc
    12241 tcacaacaaa cgtaaattgg ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc
    12301 aaattctatc tttgatatcc aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa
    12361 cgctttgtac gtgatccaca aataccttga catcaaagct ggtaacatcc ctgctcgtcc
    12421 aatcacaatc ttctttggtg gtaaagcagc tccagcctac acaatcgctc aagacattat
    12481 ccatttaatc ctttgcatgt cagaagttat tgctaacgat ccagcagtag ctccacactt
    12541 gcaagtagtt atggttgaaa actacaacgt tactgcagca agtttcctta tcccagcatg
    12601 tgatatctca gaacaaatct cacttgcttc taaagaagct tcaggtactg gtaacatgaa
    12661 attcatgttg aacggagctt tgacacttgg tactatggac ggtgctaacg tggaaatcgc
    12721 tgagttggtt ggagaagaaa acatctacat cttcggtgaa gattcagaaa ctgttatcga
    12781 cctttacgca aaagcagctt acaaatcaag cgaattctac gctcgtgaag ctatcaaacc
    12841 attggttgac ttcatcgtta gtgatgcagt tcttgcagct ggaaacaaag agcgcttgga
    12901 acgtctttac aatgaattga tcaacaaaga ctggttcatg actcttcttg atttggaaga
    12961 ctacatcaaa gtcaaagagc aaatgcttgc tgactacgaa gaccgtgacg catggttgga
    13021 taaagtcatc gttaacattt ctaaagcagg attcttctca tctgaccgta caatcgctca
    13081 gtataacgaa gacatctggc acttgaacta atactcttcg aaaatctctt caaaccacgt
    13141 cagctttatc tgcaacctca aagcggtgct ttgagcaacc tgcggctagc ttcctagttt
    13201 gctctttgat tttcattgag tataagatac aaatttatac taatacatct tgtaaaaaag
    13261 cgagtttcga ttgaaattcg cttttttaaa tgctgtggat ttgggtcaat cttgtctaaa
    13321 aatagggaaa tcctagatac agtgaaggct ttaaatgctg gtttttactg tcttcagcct
    13381 tatatttttt cgtagttggt tacctcatat ctcttgtatt cgcttacata aagtattata
    13441 atataattgt aggaaagaag gtgtttttat gatatacaca cttaaattgg tgttgtttat
    13501 tacctttctt gtaataagct tgttacctga taagattttt ggaaaaaata aaaaaatttg
    13561 gaaaatagtt tttgcaatat tgacggcagt ggcagcattg tcatttatgt actaagttat
    13621 tttaagaatg tagggaaata aaccctacat tctttttagt tttttctgtt ttctaaattc
    13681 tatttatcca agcgcttcaa catttcttgc ttcttcgctt caagttctgc acgcttttct
    13741 tcgatttcgg catgtttttt ctcgagttca gaacaacttg catcattgct aaattctttt
    13801 cgccatcagg agatagggtg agtcgacatg tctattactc acccaaagca gtcctacaaa
    13861 gcaggaattt tctgttactt ttttggaaat agtaacgttt atacagcttt gacacttcgt
    13921 atcaaagcgc caaacacact ccgagggatt tacagaaagc agaaaaggaa tgatctggta
    13981 taagatcatt ccttttctct ctttttcttt aagtaattat atacaatgta cgacgaagtc
    14041 gtcattgcaa tgctgatcca ccacctaaag ggaactttaa acaacattga taagataaag
    14101 aatataaaca acgaaaatac gttataccca attaatttta ttgtatatct catgattaaa
    14161 agttaatcct tccgttgtta ggaatggcat catttttatc ccataattgt gctaaataag
    14221 tccccggtga taataaattc atagcgaatt ctaaagcaac atcatttaca aaccaactac
    14281 ctagatatct agaaattgct gaacgaatag cactttttgc tacatgtttt ccttttactt
    14341 taattagatt tgcaaggcct gcagtagttc ctcctaatgc taaagctatt gcagtatcta
    14401 atagagcacc catttgatta actgtaatac cttgccaagc tgctctaaat ggagagtatg
    14461 taggtgggat tgtataatcg ccttgtaatt gtcggttaat tacttctttg atccattgtt
    14521 gtgagacgtc tggatgaaaa gattggattt cgtttgcaag tgtattgatt tgttcttctg
    14581 ttagagaagt gacaggttga agttccatat ttgtttcaat ttgtgatact tgttcagaag
    14641 cgtatacagc tgaaacactt ggaatcgctg atacaattaa cacaattgac gtcaaaaaaa
    14701 ccgaaataaa tttcattaat ttgtccatga gcttttctcc tttttatttg tatctgctta
    14761 cattttatca tatactgtta ttatagtcaa aaaaatatgc tattatgtta aaaaaatatt
    14821 tttcaaaata taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct
    14881 atattggtaa ccatgatttg tttactctca atcatcaaga attctctttt cgtggtagcg
    14941 tttggggtct ggtactggcc ttatatcact tattgttcat tgataagttt gtaatttcga
    15001 atcgaaaata aagattagaa ctatgattga ctggctactt ttaatattga ttttggagga
    15061 agattttgtc tctattattt attattttaa atttatttat tttgtataag atctattctt
    15121 tgaggcggga aaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg
    15181 tagtatatgg tattcaatgg ttattaagag aactgatttc aactatttcc ccttaatgtg
    15241 tatggaaact ataacaaaac gcataagatt aaggtttgtg tacctgttct tatgcgtttt
    15301 tgtcatattg aagattttcg gtaagtttta tacctttggg aagtctagtt tagaatgctt
    15361 tcccaaccaa aatctctgct tcgatggtaa tctctgtcag ttggctccag tcaatcttgc
    15421 tctggtcaac gtgaaagagg agaggagtca tactgagtag ggcttggagt tgttctgctg
    15481 tgatagtctt agtcagagag gcagtttgac tagataggat ggtaaagtgt tcctggaaat
    15541 gctccttgat atcttggtta gaatactcct tgtttgtcag ctggtcctgt actctttgac
    15601 ggatttcttt gaggtgattt tcagttggga taacctttat caagataccg tctttggata
    15661 aaacgcgacg aaattctcca tagttggcag gtgagaagat atcaagcaga atatccatgt
    15721 tagcgtcttt tataggaagt cgtgccaagt cgccaacaaa ccaattgact gcccagttgg
    15781 gttcactttt agcagcgatt tggactgaat ctttggagat gtcaaaggca tagaaagttt
    15841 tttcagagtg actttcttgt agtttgcgag aatagaatcc ttcaccacaa ccgatatcca
    15901 aaattgttgt ggtagttttt gagcttgcaa gcaagtcaga tacagcatct aagatagctt
    15961 ggtaaaagcc ggcttctagg atttgttgac ggttttgaaa attttccttg ttatagttag
    16021 cagattgctt gatttgaggt gctagattga catagccaaa tttcgccaag tcaaaagaat
    16081 gacggttgca gcacttgaaa ttagtctcta acagagtcag attttcttga cagataggac
    16141 aggcaaaggc agtcgcagaa gcaaaacgct gaagtttggg cttgagattt gtattcatag
    16201 tttaagtgta tcaaaagagg caaatgaaag caactttagg aataagtaga tgggagattc
    16261 agtagaaata aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc
    16321 tatataataa tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag
    16381 ttgcgaaaaa atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa
    16441 tcttagcaag taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt
    16501 tgaaagacga gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc
    16561 aagatgccaa atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag
    16621 gtgaataata tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct
    16681 tctttttcac ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag
    16741 gggattgagt ttatccagtt ttacatggat caacagcact ttactctacg cataaaagaa
    16801 ggcttttctg tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt
    16861 tctgagttga tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt
    16921 gcagcggtta agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg
    16981 acttgttatc aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa
    17041 ctaacgatgg aagtcttgga ctgttcggct attttattgt ctttgtttat gaaccaatcc
    17101 aagacagcta gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc
    17161 ttgaaaaaaa ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc
    17221 ctagtacagg gcgatacggt tgttagtatc aagagttccg atgttcaaat aggagatgtc
    17281 ttgatcttat ctcaaggaaa tgaaattctg tttgatggac aagtagtttc aggtttaggt
    17341 atggtcaacg aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat
    17401 ttggtttgtg caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat
    17461 cagatgaaca gccgtatttt acagctgatt aagttgatga agaaatctga agaaaacaag
    17521 aaaacgaaac aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttt
    17581 ggggctgggc tgacttacct attgacaggt tctttttcta aagctatttc tttcctattg
    17641 gtcgatttct cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag
    17701 gaagggttga accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa
    17761 gttgatactt tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa
    17821 aaggtgttac cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt
    17881 gaggaacaca tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga
    17941 attgaacatg aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct
    18001 catatagatg gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt
    18061 catatcagtt cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta
    18121 ttcttggctt atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa
    18181 gaagcaaaaa cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca
    18241 ggagacacct tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat
    18301 acagacttga aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac
    18361 actattttga tggttggaga tggattgaat gactcagcgg ctttaactct atcagatatc
    18421 ggtgtggtga tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta
    18481 gataatcgtt tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc
    18541 atcaagaaaa atattcagga taccgttgtc gtaaatagta gtttgattgg ctttggctta
    18601 tttaattggt tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt
    18661 gtcctgcgta gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag
    18721 ttacctttct cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc
    18781 aatacaaagt attttttctt cccacgacgg ataacagtca gttcattttc taacttatca
    18841 gcgtcactca agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg
    18901 ttttggacgt cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt
    18961 tccacgatat tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct
    19021 tgtttgagct ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg
    19081 ttaagtgctt ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag
    19141 actttttgag ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc
    19201 tcatcaagtg acaagaaagt aaagactttc aagaagcgaa cagcgtcagc gtccatcacg
    19261 ttcatccaga attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca
    19321 tttccttctg atttaccaaa tttcttacca gttgcatctg tgattagtgg aacagtgata
    19381 acgtgaccag tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc
    19441 cactggtcag aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag
    19501 tcgtaccctt gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt
    19561 tttttaacag attccttact catcatgtag ttgaccgtga agtattttcc aatatcacgg
    19621 aggaagtcaa tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg
    19681 ttttcgccat tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag
    19741 ccatctactg tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg
    19801 atgagacctg tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga
    19861 cgacttgtca agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag
    19921 ccagtataat aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc
    19981 gtttgaaata tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc
    20041 tttttaaaat tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat
    20101 agggaagaaa gtggctattt tattgaaaat ttcccttttg tggtataata gatagagtga
    20161 ggaaatccat gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc
    20221 aaaaacaaaa aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat
    20281 ctaaaacctt agataagtca gccattttcc cagctatttt actgagtata aaagccttat
    20341 ttaacttact ctttgtactc ggttttctag gaggaatgtt gggagctggg attgctttgg
    20401 gatacggagt ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc
    20461 aggtcaagga catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt
    20521 ccatagagag tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga
    20581 agaaggctat cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca
    20641 aggcggtgat tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt
    20701 caaccttgac ccagcaacta attaaacagc aggtggttgg ggatgcgccg accttggctc
    20761 gtaaggcggc agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga
    20821 ttttaacgac ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg
    20881 caggggctcg gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc
    20941 ctcaagcagc atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa
    21001 atactgggga gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag
    21061 ttctttacag tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg
    21121 attatgacct taaacaggac tttttaccat cgggcacggt tgcaggaatt tcacgagact
    21181 atttatactt tacaactttg gcagaagctc aagaacgtat gtatgattat ctagctcaga
    21241 gagacaatgt ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt
    21301 tggcagccaa ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa
    21361 ttcattctgc catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag
    21421 gtcgtgtaga agtagggaat gtcttgatgg acaaccaaac aggtgctatt ctaggctttg
    21481 taggtggtcg taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc
    21541 cagcttctac taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg
    21601 gaagtgaaac gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt
    21661 atgctaatag caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga
    21721 atatccctgc ttactggacc tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt
    21781 atatggaaaa gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg
    21841 gtggtattga agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg
    21901 gagtttatca tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt
    21961 atgagtatca ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg
    22021 gattgctacg agaagttcta tcctctcgtg tgacaacaac cttcaagtct aacctgactt
    22081 ctttaaatct tactctggct aatgcagatt ggattgggaa gactggtaca accaaccaag
    22141 acgaaaatat gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc
    22201 atgatgataa tcattcattg tcacgtagag caggttattc taataactct aattacatgg
    22261 ctcatctggt aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg
    22321 ctttagatcc tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga
    22381 aggtttctgt tgaaggaaaa gaagtagagg tcacaggttc gactgttacc agctattggg
    22441 ctaataagtc aggagcgcta gcgacaagtt atcgctttgc tattggcgga agtgatgcgg
    22501 attatcagaa tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca
    22561 gttcaagtag tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa
    22621 gggcgagacg ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt
    22681 tcgtgttaca ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat
    22741 tcgctcctat ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact
    22801 cattatcagg tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt
    22861 gtactcattt ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag
    22921 aaaaattacc tgcccttttt agcttatgct attttgggga ttttttcagt ccagtatttt
    22981 ttctatctct gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc
    23041 cctgtcttta tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct
    23101 gttttctatg ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc
    23161 tctcagttat ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt
    23221 atgtttaatg ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt
    23281 gggtggggga tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt
    23341 tcctttactc ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg
    23401 gcttttgctt ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggttgcc
    23461 gttatcagtg ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta
    23521 gtagtggatt ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg
    23581 tctatagaag aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct
    23641 atactagaat agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat
    23701 cggtaggggc ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc
    23761 taaaaacaga caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat
    23821 aacgaaccag gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt
    23881 tatcgccaat gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac
    23941 tgctgtgcct agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt
    24001 ggctccgctt cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg
    24061 taattctgca gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc
    24121 tattatccgt gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat
    24181 caatatcggt gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg
    24241 ccgtgctatc gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat
    24301 tgagccagct agtgctgaac cagtctgtgt cggagacaat gttcttatcg gggctaatgc
    24361 agtggttatc gaaggagtcc aaatcggtag tggttcagtt gtcgctgcag gagctatcgt
    24421 tacccaagat gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga
    24481 aattgatgcc caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt
    24541 gtaaaagtaa aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca
    24601 gatagatgtt agatttgatt cagactagac gagctttaca ccagattcca gagattggct
    24661 tggaggagtt caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca
    24721 aggattttgt tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc
    24781 agccggagcg aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa
    24841 caggtctacc ttttacttct caacaccaag gtcgcatgca tgcctgtggt catgattttc
    24901 atatgaccat tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc
    24961 tgctctttct atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg
    25021 acggtgcttt tggagattgg ttgccaaacc aattctatgg tctccatgtt cgtccagatt
    25081 tgaaagtcgg ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga
    25141 agatccgttt caaaggtaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct
    25201 tggtggcggc tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc
    25261 caatcgaggg agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca
    25321 tcacagacac agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct
    25381 tggtgcaaaa gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag
    25441 tcgaagtgga actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc
    25501 gtgaactgat ggacttcttt gacgagaaag acggaatcga gttgattgat atcgaacctg
    25561 ctatgacagg cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc
    25621 taggtatcga tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct
    25681 tagctattgg ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg
    25741 cttgtctatg aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca
    25801 agagcaaaaa caggctatag accaagcttt aaccgagcga attttacaac acccctttta
    25861 tcaagaagcc aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga
    25921 actgattgag caggcgctga aggacggcaa gaaggttttg acacctaaga cctatcccaa
    25981 ggggcgcatg gattttgtgg tctatgatcc gcaacagttg gtaaaaactt cctttgcctt
    26041 actggagcca cagggagatt tggaagtagt ggatgcatct cagattgatt tgattcatgt
    26101 tcctggcctg gcttttacga cggatggata tcggattgga tatggtggag gttattatga
    26161 ccgctatctg gaacattttt ctggtcgtgc tttgagtacg atatatcctt gtcaaattca
    26221 ggactttata cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa
    26281 tctttgatag acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt
    26341 tactaatgtt ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg
    26401 gagccatgta tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg
    26461 ccatttttgt tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc
    26521 ttggaaggca ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat
    26581 caggaatgat gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg
    26641 cctctacctc tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca
    26701 atccttatat tcaacagcta gggcaatcct atctgacact ttttgtagtt aacattattg
    26761 gaagtgttct gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg
    26821 catttctagc agttatcttt ccagttagag gagaaaaacg gatgtataat accagccaga
    26881 gattaggagc ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg
    26941 gaatgtgacg aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac
    27001 cttatctttt tattttattt tattccttct cagtcaattc ttttccaagt tggatgaggt
    27061 aattcttcaa atcatctttg acttgtgggt gtttgagggc gtagtcgatg gatgttttca
    27121 tgaagccaaa cttgtctccg acatcgtaac gagccccatt gaactcacga gcaaatacac
    27181 gttgtgtttt attgagggtg tcgattgcat ctgtcagctg aatttcattt cctgcacctg
    27241 gagcttggtt ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt ccgataatag
    27301 caaggtcgct aggagcgtcc tctggagctg gtttttcaac aaaggtttca acactgtaaa
    27361 gaccatcttt tccttcgcct tgcggagcaa taaccccgta agcagatact tcgtcatgag
    27421 ggactggcat gacagcgata gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt
    27481 tggtaagtgg aacagccttt tcgtctgtga tatccatcaa gtcatcacca agcataacga
    27541 caaaaggttc atttccgacg aaagccttag cttgcaaaac agcatctccg agaccgcgtg
    27601 gatgagtttg gcggataaaa tgcagacgca tgtcagttgt tttatcaact agcttcaaaa
    27661 gatctgtttt ccctttttct ttgaggttat attccaattc gaagtttgaa tcaaagtggt
    27721 cctcaataga acgttttgat ttaccagtga caactagaat atcttcaata cctgatttga
    27781 gagcctcttc cacgataaac tggatagttg gtttgtctac gattggcaac atttctttgg
    27841 caagggcctt ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg atgactgcct
    27901 ttctaacttt tgatgtcata aaaatccttt ctatagaggg ttaagaccac tcattttctg
    27961 ctttaaattc attgttcatg atgtcataaa tggcatcttt gatattggtt ccgtgataaa
    28021 taacttggta aatagcctgt gtaatgggca tatagactcc aagttcttgg gctagttcat
    28081 aggctgctcg agtcgttgaa attccttcga ttaccatgcc catattagct tctatatcag
    28141 ctagggattc tcctcgtccg agagcatctc cagctctcca gttacgagag tggatggaag
    28201 ttcccgttac gatcaaatct cccacaccag ataagccgct ataggtcaat ggactggccc
    28261 cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg gctgccttag
    28321 cattatcacc aaatccaaga ccatgtaaag ctccagcacc gacagcaata atatttttaa
    28381 gagcaccagc agtttcaacc ccgataacat ccgtattggt ataaagtcgg aagtagtgat
    28441 tactaaatag ctcctgaacg tattgagctg tttgtaaatc tttagaagca gcagtgatta
    28501 aagttaggtc acgcacaatg gtttcttccg catgactagg ccctgaaaca acgacaatat
    28561 cactgcggag ctgttcagga atttcttctt caaggatagt tgataatcgt ttatggctat
    28621 caggttctaa tccctttgaa gcgtgcatga tgatagcctt atggtccaag gtttgtgcaa
    28681 cttgttgggc aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc gcatccacat
    28741 ctttcaatgt ttctgctaag tcggtgtagg caatgatatt ttcatctaga acgacatctt
    28801 taaagtagtg cttgttagta tgataggtat taatttcatt gatttgctcg ggaagatttc
    28861 cccaaatacg tacctcgtgt ccattgtcat ttaagacttg tgaaagggca gttccccaag
    28921 aaccaggccc caagacggcg acggtttgtt tttccatgat ttcctccttg atagagttct
    28981 ttccattatt ctatcatatt ctagaggatt ttgcacttgc attactgggg tatggtggaa
    29041 aaatacacaa agaccgagat gaagggataa actcttagtc tttagttaaa gtgagaagaa
    29101 atctgataga tagtttgatt cagtatatgc tataaataga ggtaaacaac tcaggaggtt
    29161 cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca
    29221 gtcgttcaga attttgtggt gatgagtctg aattaagtat ccgtcaatta attagaattg
    29281 aaaatggaga atccagacca acactaacaa agttaaaata tatcgctgaa cgtttggggg
    29341 gggggtgaag attacaagtt gatgctaagt tatatagagt tggataagga atacctagaa
    29401 ttgaagtatt tcttgatgag gactcctaca tacgaagatg aaactatcgc ccaaaagaaa
    29461 gagagtgttt ttgctaagat ttttgaagag tattatgata ggctacctga ggaagaaaga
    29521 tttatcatcc caaattattc atatctagca ctagcgaact acacagttca aaaattacca
    29581 gaaaagctag ttgaaatact gtccttctgg tgattttgct taatttctga tgttcgagac
    29641 atgattgagg agatttgtct tgattttttc ctaaaaaagg gcagactcct cccttggttt
    29701 gatgctcgat ttttaaaata aggtgactta ttctgttagg catgttttcc cctgccctct
    29761 agctctatat ggaacaggga gtatcagatt tatctggcag attgtatcaa ataaggcttg
    29821 aaactgtttt gaataggcgt atagacttga gaatttgaga atatgtcgtc tagcagtaga
    29881 gacatatttt ccggcaatat gaaggaagag acgtcgaaaa cgcttgatag tcaaggactt
    29941 tacttcatca ccagctagct gctttaaaaa gaggtagaga ttataagcca gacagcccat
    30001 catcatacgt acttcattct taatcatggt cgaactatct gtcttatccc caaagaatcc
    30061 tgctttcctt tctttgataa agttttcagc attccctcgt tcacgataga attgaaacag
    30121 ctcctcacta tctacactgc ttaaattgtt caaatgaact tttttacaac cataaaaaag
    30181 agcacttaat cgtactcttc tcttttaagg attagttgaa ttaattcaaa tccactagtt
    30241 ctcctgtttc taggtagact aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt
    30301 ccagatgtcc tatgatataa aggtattgag ttccattggg aattgaggtt tcttggtctt
    30361 tcataagtcc aatgatttcc tttgataagg cataataata ttggtcaatc tgttcatctt
    30421 tttgagcaat actaatagct tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg
    30481 ctagcatgct gagggataat ttacccattt ggtgtaactg ttcttcgtca ggggctagtt
    30541 gattttcttt tagttgcaaa acagctttgg caatgcctgc catatggtct cccatacgtt
    30601 caaggtctga acaagaagac atgatgctaa tcacaaatcg aaggtcagac acttgtggct
    30661 gttgcaaggc caacaaacgg gcacaggtca attcgatagc gctttgacct tggttgatag
    30721 catgatcctt attgataatt agctctgcca tctccttgtc tttggaggct aaggccagta
    30781 aggcttttga agctgtttca aggacaagtt gccctagtcc taaaaaggat tgttctaatt
    30841 catgcaattc taagtcaaat tgatttctca taggtttttc cttccttatc cgaaccgtcc
    30901 tgaaatatag tcttctgtgc gctgatcttt tggattggta aacacgtcaa cggtatctcc
    30961 aaattcgcaa atttctcctg ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg
    31021 ttgcatgtta tgggtaacaa tgataatcgt ataatccttt tttagttgct gaatgaggtc
    31081 ttcaattttt aaagtggaga tagggtctaa ggctgaagtc ggctcatcca ttaacagaac
    31141 atcaggttct actgctaaag ctcgcgcaat gcaaaggcgt tgctgctgac cgccagataa
    31201 ggacatggca ctctttttaa gatcatcttt gacttcttcc caaatggctg ccccttttaa
    31261 agatttctcc actaaggcat ctaattgttt tttgtctcga ataccatgtg tccttggacc
    31321 ataagccacg ttatcataga tagacatggc aaagggatta ggctgttgaa aaaccatccc
    31381 tacacgcttg cgtagctgat taaggttgaa tttgctacta taaatatctt gctcatctaa
    31441 gaggacttgg ccttcaatat ggcaagaagg aaccaaatcg ttcatccggt taagggtttt
    31501 tagaaaagtt gatttgccac aaccagatgg gcctatcaag gcagtaatct gtctttctgg
    31561 taattgaatc gaaatatttt ttaaggcttg aaaatccccg taaaataagt ctaggtgtct
    31621 gactgaaaat gttcccatac taggaagctc ctttcacaag ttttcgagat aataagcttg
    31681 atagagtatt tatcattaaa acagtaataa tcaaaatcac accggtagca taggcttcat
    31741 tgacatgtag cccctcactt gacagcatat acatatgcag ggctagagaa cgacctgaag
    31801 acatgagact acttggcgta ttggtagagg tacctaatgt atacatgagg gcagctgtct
    31861 caccaacgat acggccaata gctagtatca ctccagctaa aatacctggc atggcaactg
    31921 gtagaacaat tctaaaaaca gtccgtaact tacctgcccc aagtccataa cttgcttgac
    31981 gcatgctatc actaacagat aaaagggctt cttctgttga gcgaataatg actggcaaca
    32041 ccatgatgac tgaggttaag attcctgata acagagagta ttgaaaacct aagaagacta
    32101 caaagaagag catgccaaac agaccaaaaa caatggaagg aatcccagac aaggtatctg
    32161 aggccaatcg catgatttta acacaaaggg aatctttttt tgtatattcc acaagataaa
    32221 aaccagcaaa aatccctatg ggcaaggcta aaagaagagc accaaagacc agaataacgg
    32281 tggaaataat cgctggcata agggaaatgt tctcagaagt ataagtccaa gaaaagaggg
    32341 atagacttag atgaggtaag cctttgatga ggataaaacc aatgattaaa aagagagagc
    32401 caaaggttaa agctgaaaaa caataaacga gaagttttag caggtattta ctcataagat
    32461 gattttcctt tcaagtaggc aaagtaggca ttaatcaaga gaataaggaa aaagagaact
    32521 gctgaggttg caataagggc ttccctatgc tgacctgatg cgtaagccat ttccagaaca
    32581 atattggttg ttaaggttct ggttcctgaa aagagtccac ttggaataat cggctggttg
    32641 cctgccacca aaataactgc catggtttca cctactgcgc gaccgattcc taaaataact
    32701 gctgataaaa taccagatct cgcagctggc aagatgacac taaaaatact ccgttcatga
    32761 ctagctccta gagccaagct accagaataa tacgttttgg gaactgttcg gatagcagat
    32821 tctgacaaac tgataatggt tggcaaaatc attattccta atagtaacga agcggttagg
    32881 acactcatgc catttcctaa aaaggttcta atccaaggca ccaataattg taggccgaaa
    32941 aaaccataaa caatagatgg aatggccgcc atcaagttga tagctgattt taagaagcca
    33001 tagacgggct ttggacaata ataaaccata aacaccgatg tcaagatgcc tgttggcacc
    33061 ccaatcacaa tcgctcctaa ggtaattaat aaggaaccaa cgatcattgg taaaatacca
    33121 tagcttgccg gaatgttcgt tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg
    33181 tagttagcca tgaaaggtaa gccattacta aaaataaaga aacagattag caaaatagct
    33241 acaacagcta ctgttgcact catgaaaaaa attgccctaa aaactgcttc tttgaaggct
    33301 tgttttgtca catcttgtcc tttctagtga agaaagtaag ggagatacga cacctcccta
    33361 cttgccttct ttatcttatt gtacgatgaa acgtctgcat ctctttagag atttatggag
    33421 caaacatttt atttaatctt gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt
    33481 tcagccatac tgacttggct tgccttattg tcattattga ccacaacagc aataccgtct
    33541 aaagcaatag catcatgggt gagactctta ccttcttcag gagttaattc cctagaaacc
    33601 ataccaatat cagcggtttt ctccttaaca gcggtaatac ctgctgaaga cccattagag
    33661 gtaatatcaa tcgtaacttc tggattttct tttttataag cttctgctaa tttttccatt
    33721 aaagaagata ctgaagtgga acctacaaca gacaacttgc ctgataagtg ttggcttgta
    33781 tattctgtgg tttcggtttt agcttcaata aatttattat ctgtgaccac ttgttgacct
    33841 tgtttggagt ggataaagct gataaaatct tgacctagct tggaaagatt agaagaccaa
    33901 acaatgttga agggacgttg aagagggtat tcaccatcta aaactgtgtc tcgactagcc
    33961 ttgacaccat caatctctaa agccttgaca gatttcgtta aagatcccaa ggagatgtag
    34021 ccgatagcat tagcattccc ttgaactgct gagagaacac cttctgtact attttgaatc
    34081 acagctgttt tggcagtgtt gtcaattttt ttatcaccgt cttttttgag aatccctgtg
    34141 atttctgtga aggcaccccg tgttccagag ccattttctc gtgaaatcac ctcaatcgtt
    34201 cctgaagctg actgtttgga agcagctgac tgattgccac aggcaacaag cccaaatcct
    34261 gataagccaa tggctgcaag agtaagcatt tttttgaatt tcataataat cacctttatc
    34321 tctatgtatt tttcttgtgt aggcttacta catttatagt ctaacaagtc tttgtaaagg
    34381 tttatccctg attcatgtaa agattgtgta aagaatcaaa aaaagccact tttgaaaaat
    34441 ggctgctcct aaaaatagct ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag
    34501 aaaaatcgtg aagcaactgc ctctgccaag ctgactcgtc accgtgactt ggccacctaa
    34561 taattgactg agttctttga caatggcaag gccaagacca gtgccaccag tttgtctgct
    34621 tcgaccttta ttaactcggt aaaaacgttc aaaaatacga tcctgctcta attgactaat
    34681 accaatccct gtatctgata cagaaatctt aatgccttcg ttcacctttt gggtcttgac
    34741 ctcaattttt cccccttgtt cagtgtaacg gatggcattg gataaaagat tgagtaagat
    34801 ttgggaaagt aattgactat ctgatacgag ggtgacatca tctggcacct gcacctttag
    34861 ctgtaaatcc ttcttcttga gctgaggttg caagctttga gtcaaatcct gtacaaattc
    34921 tgccaaagaa agggtcgtcc attgtatagg catttgttga gccttagata aggtaagaag
    34981 atgctcaaca atatgctcaa gacgcaagct ttctttgtaa ataatgtcta gaaagtcatc
    35041 cttgagcgct tcttcttcag ctgacatccc cttaatggtt tcagcaaagc ccttaatcga
    35101 agtaactggt gtcctcaatt catgggaggc atttgagaca aaggctaaat ttaacttttc
    35161 ataagttcta atcgttgtta aatcatatag caagacgagc acagcttcca cagattgggt
    35221 ggggctaaaa acgggaactg ctgtcacttc taaaatcaag tcaccctcat gaaacccact
    35281 tacttcttgt tttaaccttg ttttttgatc aaaggcttgg tgaactaaat ttcgaatatc
    35341 catccgtttg aggtcatcaa gtgaacttat gtcgccgtcc acatcgggaa aataatgagg
    35401 cagagagcga ctggataata acatctgacc ttgagcggaa actaaaaacg tccccatggt
    35461 taggtgcgac agaagaacct ccattgtttc ggctaggtcc ttgtattgct gatcctgttg
    35521 ggagactttg gtttttaggg cagacacata ctgagccaaa gactttaagt cttcttgccc
    35581 tttttctaaa aagtattcac tactggtcaa gagaggttgg tgcaaggtct caaaagcaac
    35641 ttcccatttc caaaggcaaa agagccagta gccaccttgt cccaaagaaa gggctagaag
    35701 aaagagaccg atgcctttac tgatccaagt taatgccatc cctgcaatca gaatgaggct
    35761 aacacttaga ttgactagcc aaaattgaag gtagcgtttc atctataact ccttgaactt
    35821 ataaccataa ccccgaatgg ttcgaataaa ttgaggggct ttaggattgt cttcaatttt
    35881 ttccctcaac ttaccaatat gaacgtccac caaacgtgtt tcctgcccaa agtcataccc
    35941 ccagatacgt tccaaaagac gctctctagt cagtgtcatg ttgggatgtt tcataagata
    36001 gagcaagagt tcaaattctt ttggggtcaa actcagtaac ttattcgcct tgtagacttc
    36061 atgacgctca gggtatactt tcaaggtccc aaatagccaa gaatcgtcag cgatattatc
    36121 tgaatcatct ccttcttgtt ctcctttagt tcgcctgagg acagccttga cacgcgccag
    36181 caattctcta gggctaaaag gcttggtcag gtagtcatca gcccctaatt ccaaggccaa
    36241 aaccttatca aattcatcac ttttcgcaga aaccatcata attggagttt tgacgccttt
    36301 ggctctcagc cgcttacaaa cttccatgcc atctaattgt ggtaacatga tatcaagcaa
    36361 gataaaatca aagggttctg tttctgccaa agctaaggcc ttccgtccat ttgttaccaa
    36421 ttgagtagaa aagccttcct tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc
    36481 atcatccact aataagactt gttttgtcat ctattatctc ctattggtaa cattataaca
    36541 caattatcag aaatcctaac attgctaaat cagattaaat ttgcttatca agactagtat
    36601 ctggtcaaac gctcaatcat ctccttgtgc tctggatagg tcgccagtag atctaccctt
    36661 tcaaataatt caaaatcctc aaattcaaaa ccaggagcaa caagacaaga aaccagagca
    36721 taatccttat caactgttga tccccaaata gtgcccttag gaacacagta gtgaagttgt
    36781 tgccctttgg atatgtccag gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc
    36841 atgtgaacag taagtgggga tcctgcatga aaataccaga tttcatctgc tgtcaatcgg
    36901 tgaaaatgtg aaggattcgt ttcttctaat aagaaataaa tactggtata aagcgccctt
    36961 cccttaccag caaggtttat agtgtctgaa gctttttttg tttgtctaaa atagccacct
    37021 tcaacatggg gagctaactc tagagttctt atcaagtctt ctttatccgt cggagccaat
    37081 gggttgaagt aactcttgtt caaaggtggt gttacgattt caagaactcc tctcagctct
    37141 gaggacacga taatgattga tgcgacggaa gtacaaatca atcgccctaa aaaaagaatt
    37201 agcgaattat tctgg
//
LOCUS       DAVBCR010000013        36136 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCR010000013 DAVBCR010000000
VERSION     DAVBCR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.013"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(74..154)
                     /locus_tag="VQ508_000584"
                     /pseudo
     CDS             complement(74..154)
                     /locus_tag="VQ508_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(209..433)
                     /locus_tag="VQ508_000585"
     CDS             complement(209..433)
                     /locus_tag="VQ508_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2489629.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(438..617)
                     /gene="asp4"
                     /locus_tag="VQ508_000586"
     CDS             complement(438..617)
                     /gene="asp4"
                     /locus_tag="VQ508_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2489630.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(619..1956)
                     /gene="gtfB"
                     /locus_tag="VQ508_000587"
     CDS             complement(619..1956)
                     /gene="gtfB"
                     /locus_tag="VQ508_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2489631.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(1949..3460)
                     /gene="gtfA"
                     /locus_tag="VQ508_000588"
     CDS             complement(1949..3460)
                     /gene="gtfA"
                     /locus_tag="VQ508_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2489632.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3472..5844)
                     /gene="secA2"
                     /locus_tag="VQ508_000589"
     CDS             complement(3472..5844)
                     /gene="secA2"
                     /locus_tag="VQ508_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2489633.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMIGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5837..6277)
                     /gene="asp3"
                     /locus_tag="VQ508_000590"
     CDS             complement(5837..6277)
                     /gene="asp3"
                     /locus_tag="VQ508_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2489634.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6274..7809)
                     /gene="asp2"
                     /locus_tag="VQ508_000591"
     CDS             complement(6274..7809)
                     /gene="asp2"
                     /locus_tag="VQ508_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2489635.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7793..9373)
                     /gene="asp1"
                     /locus_tag="VQ508_000592"
     CDS             complement(7793..9373)
                     /gene="asp1"
                     /locus_tag="VQ508_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2489636.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9383..10600)
                     /gene="secY2"
                     /locus_tag="VQ508_000593"
     CDS             complement(9383..10600)
                     /gene="secY2"
                     /locus_tag="VQ508_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2489637.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10657..11334)
                     /locus_tag="VQ508_000594"
     CDS             complement(10657..11334)
                     /locus_tag="VQ508_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2489638.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11319..11564)
                     /locus_tag="VQ508_000595"
     CDS             complement(11319..11564)
                     /locus_tag="VQ508_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2489639.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(11564..12760)
                     /locus_tag="VQ508_000596"
     CDS             complement(11564..12760)
                     /locus_tag="VQ508_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2489640.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12769..13989)
                     /locus_tag="VQ508_000597"
     CDS             complement(12769..13989)
                     /locus_tag="VQ508_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2489641.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(14020..16464)
                     /locus_tag="VQ508_000598"
     CDS             complement(14020..16464)
                     /locus_tag="VQ508_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2489642.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16474..17484)
                     /locus_tag="VQ508_000599"
     CDS             complement(16474..17484)
                     /locus_tag="VQ508_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2489643.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(17513..18388)
                     /locus_tag="VQ508_000600"
     CDS             complement(17513..18388)
                     /locus_tag="VQ508_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2489644.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18428..19306)
                     /locus_tag="VQ508_000601"
     CDS             complement(18428..19306)
                     /locus_tag="VQ508_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2489645.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(19303..21388)
                     /locus_tag="VQ508_000602"
                     /pseudo
     CDS             complement(19303..21388)
                     /locus_tag="VQ508_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21449..24103)
                     /locus_tag="VQ508_000603"
     CDS             complement(21449..24103)
                     /locus_tag="VQ508_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2489646.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVMRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNDTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASTSISASSTVVGSQTAAATEATAKKVEEDRKKPASDYAASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIAKSETKVYTGTGLDSVYHVPIY
                     YKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHGYSIYNTGTSIQTMLTLGSDLG
                     TPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNGAQMNGFFAKQGYGLTSSWTVP
                     ITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTTSQSLSQSKSLSVSASQSASAS
                     ASTSASESASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASAS
                     TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTS
                     ASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSAS
                     ESASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASAS
                     ASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAS
                     TSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTS
                     ASESASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQKELPNTGTESS
                     IGSVLLGVLAAVTGIGLVAKRRKRDEEE"
     gene            complement(24341..24802)
                     /locus_tag="VQ508_000604"
     CDS             complement(24341..24802)
                     /locus_tag="VQ508_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2489647.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(24769..24912)
                     /locus_tag="VQ508_000605"
     CDS             complement(24769..24912)
                     /locus_tag="VQ508_000605"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2489648.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            25181..25561
                     /locus_tag="VQ508_000606"
     CDS             25181..25561
                     /locus_tag="VQ508_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2489649.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            25660..25881
                     /locus_tag="VQ508_000607"
     CDS             25660..25881
                     /locus_tag="VQ508_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2489650.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            25898..26212
                     /gene="trxA"
                     /locus_tag="VQ508_000608"
     CDS             25898..26212
                     /gene="trxA"
                     /locus_tag="VQ508_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2489651.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            26519..27052
                     /gene="queF"
                     /locus_tag="VQ508_000609"
     CDS             26519..27052
                     /gene="queF"
                     /locus_tag="VQ508_000609"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2489652.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(27306..27974)
                     /locus_tag="VQ508_000610"
     CDS             complement(27306..27974)
                     /locus_tag="VQ508_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2489653.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(28085..28639)
                     /locus_tag="VQ508_000611"
     CDS             complement(28085..28639)
                     /locus_tag="VQ508_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2489654.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(28885..30681)
                     /gene="pepF"
                     /locus_tag="VQ508_000612"
     CDS             complement(28885..30681)
                     /gene="pepF"
                     /locus_tag="VQ508_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2489655.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(30692..31435)
                     /locus_tag="VQ508_000613"
     CDS             complement(30692..31435)
                     /locus_tag="VQ508_000613"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2489656.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(31437..32387)
                     /locus_tag="VQ508_000614"
     CDS             complement(31437..32387)
                     /locus_tag="VQ508_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2489657.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(32525..32953)
                     /locus_tag="VQ508_000615"
     CDS             complement(32525..32953)
                     /locus_tag="VQ508_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2489658.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(32934..34022)
                     /locus_tag="VQ508_000616"
     CDS             complement(32934..34022)
                     /locus_tag="VQ508_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2489659.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(34041..34511)
                     /locus_tag="VQ508_000617"
     CDS             complement(34041..34511)
                     /locus_tag="VQ508_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2489660.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(34802..35254)
                     /locus_tag="VQ508_000618"
     CDS             complement(34802..35254)
                     /locus_tag="VQ508_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2489661.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(35291..35470)
                     /locus_tag="VQ508_000619"
     CDS             complement(35291..35470)
                     /locus_tag="VQ508_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2489662.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            complement(35607..35786)
                     /locus_tag="VQ508_000620"
     CDS             complement(35607..35786)
                     /locus_tag="VQ508_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489663.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(35951..>36136)
                     /locus_tag="VQ508_000621"
     CDS             complement(35951..>36136)
                     /locus_tag="VQ508_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489664.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT        11162 a         7916 c         6090 g        10968 t
ORIGIN      
        1 caaaacggtg ttttgagctg acttcgtcag ttctatctac aacttcaaaa cggtgttttg
       61 agctgacttc gtcagttcca tccacaacct taaaacagtg ttttgagcag cctgcggcta
      121 gcttcctagt ttgctctttg attttcattg agtataaaaa agaaaacttc agaccaatcc
      181 acaaaaggtg aatatctgaa gttttctttt atttataagt aatcaccata aaggttaata
      241 gtagaacaaa taaagccaaa ctcaccaata aagtgagcac cttgaccaag gtgttgctct
      301 gccagtagct tggtttacca atattactag tggcatccat ggaaaatagt tgattttgac
      361 ggggttgtat agttaccaat acaatcaaag caagagatag gataatagct aaaacaatta
      421 gcatttcaat cattgatcta cctcaaaatt cccaacaaag taaagagcaa accaatcaga
      481 atcattaagc ccagtaaaag tgaaaaagtt ttactaatct tctcccctcg ggtttctttt
      541 tccttcttga tgggtttttg tttcaactct tccatgcgac cttcaacgtc tttgtaaaat
      601 aaatcttcct ctgacatgtt agcctcctaa aacagtttgc attatttctt gatatcgcac
      661 caagtcaaca taatttgcat gttggccttg tttgccaagt gcctgacgca tttgatcaac
      721 atctgaaagg gccaatttaa tggtctcaac caaggcagca acttcactac tttcaaatag
      781 atgttctgga gcgatataaa gtctattgtg aaccgtttgg ttaaatccaa gaatcaagag
      841 attgtgctca aaggcctgac gcactgcctg caacaactca ttgctgtgat tgatatccaa
      901 gtaaatatcc gacagttgat acagctcctg aatcttctgt ggactagcat tctggtaaag
      961 gaccacatta ggatagcgaa gcatgtctaa gagcttagaa gacatctctg tcactgctgc
     1021 gatacggaag gtaacatcag gcaaggcttc tacgattgct tctacttgct caatctgatc
     1081 tgaattggtc aagattaagg catctcgtct taggaaatta tcacgtttga actggtaatg
     1141 ataacccaag tgcacaaact gatcatggta tttctcgtca gttaactcta aagcgcgctc
     1201 ataagtcgcc ttatttggaa tgatgatctt cttagtacgc acattatcac tttccaaaat
     1261 caactgcata ttacctggaa tggcatcata gagaggttcc tgccatacca agacatccga
     1321 gccagattta tctggatgat ggaaggaaac caagaaagga gtcgctagag tattaaagat
     1381 aagctgactg gtatctattt ccaaatcttg caaaaagaag gtgataaatt caactttatt
     1441 tgcaaagtaa cgcatggact gaccaggcaa agtcaataag atatcacccg tcacatggtt
     1501 ttccagtaaa acttgttggc cattgacatc ttggtaaact gtcataatcg gctcgctatc
     1561 tgcgctataa gtggttttag caaagcaagc accaaagcga ttgtagtggt caacctgacg
     1621 tactcgacct tctaggtctt tccagtctac ctgtttaacc aagcgagcct gcattccatc
     1681 aacataatga atgacagccc tctcctgcgt cacatcttca atacaagcag actgattatc
     1741 tcctaaaatt tcccaaaaat ctgaaacggg aacttggtta aaatagagag gttttccatc
     1801 ctcgtaacct agataatagg taaaaggaga aagcagacca tcaggcagaa aaccatctgc
     1861 atcgatgacc actccaagtt gagaaagacc agtagcgact agactttcat gtaaatctct
     1921 actttcttga ctgtaactat cataaagttc aatcatggag cacctcctct actgttttct
     1981 tccacttttc taaaatttct ttggtcaaga agccttctgc aatttgataa gaataggcac
     2041 gcatagcttc caaacgattt tcttgataca attgacaaat cttagcggca taagcttgct
     2101 tgatttggtc ttctacatgg tcagatgaac ttggaatcaa ataaccattt tgcccatcct
     2161 ctataaaggt ctgattacca taaggcacat caaaaccaat tagaggtaga cctgaaccaa
     2221 tagcttccat caaggtcaga ccaaatcctt cgctggtaga agccgttaag tagacctcat
     2281 actggctata aatctgcgaa agttccgcat gccccttgag ttggatatag tcctctgcct
     2341 gatgatttgc aataatttct ctaagcagag aatcttctcc accactacca tagatatcaa
     2401 aggttagttc cggtaactcc ttatgagctt caatcacagc tttcacaagc caatcaatgt
     2461 gcttttcttt ggcaagacgt gaagccgtaa tcaatgaaaa tggtttgcgc ccttgacttg
     2521 aatctgtcaa ggaatcaata ctgcctacag gaatggtaac aatctttggc tgatgctgag
     2581 tatatttggc aaattgctct tgtagaactt cattttgtct atcagtagac acgataaaga
     2641 agtcaacctt atctgcattg gtaaactgat agtcataata gttattccaa aggatatagt
     2701 cctcatttgt agcattttca ctataatgct ccgcatgaac aactaccgct agatgtgctg
     2761 tctgtgcttc ctcaaacaca acctgtccaa tacctgtctc cctatccaga ataaccaaat
     2821 cagacttgtt caaattcaaa gatttcataa aggcacgcac aaaagcttgc tttccataga
     2881 aaatcttatc cttgaaatga taaacttctt ccttcccttg attcatcaaa atatcataga
     2941 ctggagtccc gtcttcattg taaaaagttc gttggtataa gactgcaaca ttgtccttgg
     3001 gagcaaaata ctcgctacaa taacgcgtat aagaaaagta atccttccga atcaggtttc
     3061 ccttaaaaac atactcggca tgttgaacca agtccttgtt ctcatcaacc aaataacagg
     3121 ttacaaactt atcttggtca aaaaagaata cacgtaaaac cttgccattt ttttctctgt
     3181 gactttcttc accaccaaag taagccaaga catcatccac tgtcacgcta gtaggtgcaa
     3241 ttttgatatc tgtgaaatga ttataaagcc agataacctg attatcatca aaaccaatat
     3301 tggctgttaa gtgctgaata ttatcggcta aaatcatatc tgtaaagata aacttagagg
     3361 acagatttaa tttccgaaaa acaccagcac gataggcttg agcgtattca acaccgctac
     3421 tagcccaacc aattcctaaa tttatattgt aaattgtcat attttctcct cttatggaaa
     3481 atgagtcacg atttcacctt ttggagtgac ctcaaccagc cccatcagga gattacgcac
     3541 catatccgca cggatttgtt ctttcatagc ttcaaagccc gcgtaggctt cttgatagta
     3601 ctctacgatt ggatttttct gactagcaga ttgaccaccg atagccatgg atagctgttg
     3661 tagatagtct acctgctcta cccagttgtc atcaatggct ttaagcagtg aaagtcgtaa
     3721 aaactgttca tataagtcat gttgattaag taattctttc ttttcagaaa gttctttatc
     3781 aatcacctgc ttcataaagc tacgaactgc agttttgtca gttacatcta tataatctgg
     3841 aacctcttta acatgaaaac taatattggt cacaataaag tgaaacaata attcacgact
     3901 agcatagtga tcagccgcta cctcttctgt atatctctca atgatatcca caacaacatc
     3961 ctctaagtca cgagaaccat ctattagacg atttctctct ttatagacta tatcccgttg
     4021 tatattcata ctttcagcat actccagagt ctgacgacgt gctgaacgtc cagcactatc
     4081 actggcatgc tgagcctttt cgactagttt ccggtattta cgacctttca atacttccgg
     4141 ttgagtcata tcttgaacct gatagtcttt gtacttttta tgcacccaag atggaccaaa
     4201 tttcttgata acatcatcct ctaaggatac aaaaaattta ctcataccag gatctccctg
     4261 acgaccagaa cggccacgaa tttgtaggtc gatccgctga ctttccatcc gctcagtccc
     4321 aataacaatc aagcccccaa gctctgcgac tcctttacca agcttgatat ccgtaccacg
     4381 tcctgccata gaggtagcca ctgtcacagc ccccatctga cctgactcgg agataatctg
     4441 agcctcacgc gccgcattat tagcatttag gacattatgg gcaatccctt cacgaaacaa
     4501 gagagacgaa tagagttgag acatttcaac tgagcctaca aaaacgagta aaggatttcc
     4561 cttagcatgg tattgcttga tgtactccaa ggatgcatac actttttcag gtaaagtgat
     4621 atatagatta tctggatagt caatccgttg tctcggacga ttggttggaa tgcgtactac
     4681 agacatattg taagtttcaa taaactcttt ttccgcgacc ttacctgtcc ctatcatacc
     4741 agatatctta ttaaacatct taaaaagact ctgataggtg atcgaggcca tagcccgcgt
     4801 ctcaggagat aatttgacat gttccttggc ttcaatagcc tgatggagac ctccttgaag
     4861 tttagtcatt tccattagac gccctgttcc cttatcaacc agtaccatct catttccacg
     4921 aatgatatag tccttatctt tagtaaagag cttatgagct cgaatcgcat aaaccaaatg
     4981 acgagcaaaa gacgcatgct cttccttgta taaattatca atccctagga aattctcagc
     5041 agacttggcc cccttagtag tgagccaaac ctcctctttc tcctctttaa agatataatc
     5101 ctctccttcg accaaggttg ttacaagtgt atcaatgatc gcatagtaat tagactgaac
     5161 acgaggagaa cccgcaataa tcagaggagt ttgtgcacta tcaagcaaga tatcatcaat
     5221 ttcatcaata ataacatagt taaacggtcg taaaaactta ccttcttcat tcgaggctag
     5281 gttatcattt agataatcaa aacctaaact actattggtt gtgtagatga tatccgaagc
     5341 atagataagc ttcttttctt cagctttcat ttccttcttt ggatcttccg taaatggtac
     5401 accaatggtc aatcctagaa aacgataaac ttggcccatt tcctcggcat cacgctttga
     5461 taaatactca ttaggagtca caaccatcac tccttctcct gaaaaagcgt tcaaatagac
     5521 aggcatggta gctgtcaagg tcttaccttc ccccgtattc atctcagcaa catttccata
     5581 gtgcatgaca atagctccca tgacttgaac atcatagggg aacatcccta aaatccgctt
     5641 atctgcttca cgcaccacag caaaagcttc aaccaaaata tcgtctagac tttctccctc
     5701 ggaaagacgc tgacgaaact ctactgtttt agctactaat tcttgatccg aaagagagga
     5761 catcttgcct ttcagggcat taatctgctt taaaatcttt ttcactttcc taagctggaa
     5821 atcttgacct aaacgtctaa acacttctga tctccttgat agaaaaatga taaaaagtta
     5881 aggactcaaa tccagcactt aaaagagaaa cttgataagt ataactatct tctggataag
     5941 taaatatgaa atctttcgct ttttctacct ttttttctaa aatatcacca tagcggtcaa
     6001 aaaaagttat cttggtataa actccatttt caggctgaca atcaaaaacc agtgatagtt
     6061 gatactcctg ctttcttttt aataagggaa gctgaggttg ggttcgtgca gcctggtaat
     6121 tgacactaga aaaccaagtc tttagaatct caccagatgg caatagagga ttatacagac
     6181 gaacactttt gtcaaggtaa taagatacag ttgttccata catataagtc ccaccaactt
     6241 ctccccaata aatagactgt ctctgtgtaa taatcatctt ttctcccttc caaaaccagt
     6301 ctctaagacc attctataaa agtgcaaaaa ccaagaaata ttggtatctg tatcatcatt
     6361 gtgtcgtcca gcagttcctt tagataaaat cttcgcacct gtattacaca gataagacac
     6421 taattgttca taggcttgtg gatccatttc ttcatctttc atataggata aaccaaaggt
     6481 tgtctttgaa aaatttgctt gtttaaaaat tttccaaaaa cgctgatcca actccgtcat
     6541 atcttgagaa cttgtcccac ctgtatgatg aattaaacaa tcaaaagcta agttagagac
     6601 acccggtgcg tccaaacgtc cacgactagc tatagttcct aaattagcca agggcttacc
     6661 aactatgatg gcatggggtt caaaagaagc accataatag agagccggaa acgttcccat
     6721 agaaagacct gataaaatca aatccttatg atcaaagcct aaataatcaa gatagtgagt
     6781 gatcgtatcc tttacttttc cctctagctc atccgttccg agataaaaag ctcccccctc
     6841 taaacgtgga tcagaaaata aaatgaaggg acatccaaga gttttcatca taaagtaacc
     6901 ctcgaatcct tctgcaggtc gataacctgc aaaatagaca gtcaaaggcg gtttaaaatc
     6961 acctggatgg aagaaatagt ttatttcatc acgcttggaa tcatgtagga tatttccacc
     7021 aagtacaaac ttaccaaatt gttttcgact ccatcgttgg tgaagattac ctagtttgat
     7081 agttccacga cctcttgctt caacagatat cgagagaaag tagtctccgt ctcgactatc
     7141 cataaaaagt gcttgctcca agtctttttc agatactatt acttcttcaa agaaatcatc
     7201 aacagaccca ctccacattt tacggattac taaacgaaag tcacaattgc cttccttctc
     7261 atattcaagc cacaactcaa tagggagtgt tttttgcacc ataatattat aagcccaata
     7321 ggcaagctgg gcaaaatctt gcccaaactc accttccaaa ctgacataat ccaatccttg
     7381 ataagaaata gctcctgtaa aatttggatt gacttgtatc gtcggaggaa agagtttatc
     7441 accataccca ccgccaaaaa gagatttact taaatcattg attagttgtt ggggacttaa
     7501 aaaatcaatt gcttgtgcac atcgtttttt tagaaaatcc acaacctctc tatcatttat
     7561 ttgtagattc tggttataga acaaagtata aggttcaaaa tattttacat aggaaaataa
     7621 gtctctggaa tactgaccat cttctattaa aactgcatga aaacggttaa tatcctcttt
     7681 ttcgattatt tcacgaaggg tttttgatga gtttgggtaa aaataatacc aactcatatt
     7741 ttcaggtatg tcatagtgat tttcccaatt ggcaagtcct atctgtaaaa tattaagttc
     7801 ctttgacatt tttaacctcc tctatccatt tctctaattt tccaagaaat tgttgtcctg
     7861 tgtgctcctt aatcttatca attgaatata tcaaggactc attccactcc ttcaacctat
     7921 ctgtgtaata atgtgcagcc ttagaaaatt ctgtcacatc tgtcagcaaa taaccatttt
     7981 ttaaatgttc cacatagacg gtttcaacta ggttgatttg aggaattcct gcactaatcc
     8041 cagcaatctg tgtgtagaga tgaggatgtc tatttaaatc cacaatcaaa cggacaaatt
     8101 ctagtgtttt tatcaaatct aactcatcat tcaagttaac aaactgtaag cgtaagtcct
     8161 ggtgttgatt ttcttccagg ggattttctg catcaccata atcaatcgcc tttcccagat
     8221 tttcggattg aatgttttct tggatgaaag actcaacaat cccttcaacc tcattcattt
     8281 gctcctgact agcagcaaag gctccaaaaa tcacctcagt atccttattt tcagcgacaa
     8341 aggacaagac ttgaagcaga gcttgattat caatcccctg ctcaaaatcc agttgaaaat
     8401 aaatgatgga ttctttctta gtttggcttc gtcccaatcg taatcgggtg tcaaaagatg
     8461 ataaatgata acattgatgc attcgttcag gatacaactc ctgcaacaat cgtaaactat
     8521 cctctctatc caccaaaatc aaatccgatt tttcaaaagt tacatctaaa tccctaaagg
     8581 tatcttgagg attacgtcca atgaacagac tcaacagttt aggattagta ctaggtaaac
     8641 gatctagtac caactggtca tgatgagaat gagaaggaag cataaaaata tcctgatcct
     8701 tcacatacgt ttgcagataa ttctcaaaaa attctgccac cagatctccc atattttgat
     8761 aggtaagcat tttaaagcga taacccaaaa ttggattgac ttctacctgt cctccttctt
     8821 ttaaacgctc tctaaattgc caaactccct taggattcaa atattcttga taggctgctt
     8881 gcccatcttc aaaaaaaata atactcgata taaaccctcg atcatccatg atatggtgca
     8941 tatgtatttg accatttggt tcaaaatact gtatatcact gatgaagcct tcaactccat
     9001 gttcaacctt agcaaatttc ttcccatttt tttgaacgat aatcgtaaag ggactataaa
     9061 taaattcaca gtcatcatcc cactcaatat ctctaatgct taaaacttgg gtatggagat
     9121 tatgaaaatc ttgcataaca tcaaaaacgg aataagtatc cgtttctaac acaccatgtc
     9181 tatataaaaa ataacgtaga tgaggctggt aagctaatac taatagacga gaatctatat
     9241 cttgctcttg aaagagccgg atctggtgaa aggtatcatc aaactctaga cgaaaatgag
     9301 aaaaatacca tggagtgata tctgcatgcc atgttctttc tgacccatac caagctggaa
     9361 taaaataata cataaaagtc tcctaaatca aaggtttata ggtctcattt agccttaaag
     9421 ccttgacctc gtctctaatc gtaaaaatca tgcctccaaa catcataaac aaacctggaa
     9481 tcattgccaa tcgtaataac ttttcatcaa acaaaacaaa aagcatggga ccacctgcca
     9541 taaccacatt aaagagacca cctatgactg agaaacgaag gaccaatcga ttaataaatc
     9601 gactagtatc cgcacctgga taaataccat aaatgtattc tccagatttt ttcatacggt
     9661 ctgcaatctc ttctccattc atcgtaacaa aagcaaaaat gatactaaat aaaaataaga
     9721 ccgaaatata aacatagacc cacaaaggct ttccaatcat aaattccttt gataaagccg
     9781 ctaaccctga atgattaggg aaaataaatc ccaacaagat gaacaaataa gctggtacac
     9841 taagaaaact catcacatac atataaggca tcccacctgc aggattcaac atgatttcga
     9901 gataaaaata gcgtttaaat cgattgtgta agccaatttt attaacagga accaaatagc
     9961 gagctcgata cataagggca agtaaataag aaaagacaag ggttaataaa gcaagtaaca
    10021 caataatccc tgttggaatg tgtactgtct gaaatgtttc caaaacatcc tgaggaatat
    10081 ttaaaaccat actggatagg agaatgacta tagaacctcc aatccccata ctcgcattta
    10141 aatctgacaa ccaaacaaga aaaaatgttc ctgctatcag caatattgta ttcattagaa
    10201 caaccaatat tgcagaatag gaggattgta ctggcagtct caagctaact gccaatgatt
    10261 gaatgacagc tatcatcaag gtcaggtaca ttttacggcg atcttgtatt tctatagacg
    10321 tagatgttaa acccaaccgt ttagaaaaag aaaacatctg ccataaaatc atagctgaca
    10381 tccaagggga caatccaaca gaaaaaattg acaaacttct tagatttcca cctgttaggg
    10441 cggctgagaa ggctagatag gctgttgaac ctcctaaaaa atctttagta tttaggtcaa
    10501 caaaagggag agtaatacga cttcccaata catagataaa cagtagaaat aaggtaaata
    10561 gtcctttttt tactgctatt gacgaataaa tcttagtcat aaaacactcc cctcaccttc
    10621 attgtttgtg atatggtaaa taacaattct tttatttcaa cttttaccat ttctttgttc
    10681 tgcatcaaaa aacaacttta atctgaaaca aatatcttta tattcctcag tatctgttaa
    10741 ttgatggtat tctaaagttt gcttataatc ttttaaaaga ttgacaaatc gaatacgcag
    10801 aacggagaca tccaaaccag ccaagacaca atccgatatt ttcttagaaa acacttcaac
    10861 aatatctctt gcccaggaaa gatcaaattc cgttctcatg atgcttcctg aacgtcttct
    10921 atacagatag agattatcgt ttataaagac gattttagag gctaaaagat aaaagcgatg
    10981 catagttgct tcatcatcaa agcggcgacc atttgaaaag tgaacttcat tgaataattc
    11041 ttttttaatc aacttaaatg tcggcaatat aaaggccgag ctattgaatt tccaatctcc
    11101 tgcttgacgg ttcataattt cttgtatagc taattcttcg acgcaaaaat cttgccctgt
    11161 tacataaaaa taaaaattcc cgtcactttc gtcataatta tagtaattcc ctataacaac
    11221 atctgcatcc ttttcttgga cttgctgata aagagtttca atcatatttt cttttacgat
    11281 atcatctgaa tcaacaatcg tgatgtactc tcccttagct acactaatcc ccatattttt
    11341 agcttgtgct gccccccccc cttattttct tgatgcaata ctcgcacacg attatcttgc
    11401 atcataaaag catcacaaat ttccccagaa ttatccgtag aaccgtcatc aactaaaata
    11461 atttctatat tttgataggt ttgcttcaga atatgctcga cacactccac taaatatttt
    11521 cccgtattgt atatcggtac cacaacacta attaattcac tcattactct atctctatat
    11581 tcttcattct atccactaaa tcatctggtc tctccacaga aaaaataccg tcatagagtc
    11641 catcatccat acttttacgt gtgatatcaa aagcgaagat tttcttgcca ctttcttgtg
    11701 cccttgtaac tacattaaaa acctctccac ctgtattaat atctaaataa actatagaat
    11761 cgtccaatag ccaatcaatt ctactatgta atacattttg atatactgtt acattcgtat
    11821 actgtgatag agaggtcagc tcctcagaac aatcacacgg tgcagccaaa tgaaaatgcc
    11881 aatcaggtaa ccgttgtact aaatactcta aatgttttat atctgcactc catgtcacaa
    11941 gcatcacttg ttttttagac tgattgcttc tttcaaaata atttaaatcg gaacgttgaa
    12001 aagcaatctc tgaccaatcc aaatctctat aaacccacca taattcacgt agtctagata
    12061 tactatatgt attccaaggt ttatcatgac tagcatagtg aacaattgta ggtggattgt
    12121 catctagacg ttcacattct tgagcaaggt gataaatatc aacacccacc atataattat
    12181 atgttttatc taaggctagc caattatcct caaaataaat atttaaaata ctctgatccc
    12241 caagatgaac aacttgattt tgctcggccg ctaattccaa taaactattg acaatagagt
    12301 gttctttcca ttttgcaaca tccattagta acataccagc attaaatcca gattttcgtc
    12361 cttcataggc atagacatca tcaacagcac caattgaata tcctttgaga tctatctcaa
    12421 acaaagtagc tagttcccca gttacaatga tatcggaatc cagatacaat accctatcag
    12481 attcaaccac ttctgtcgca aaaaatctaa agtaagaagc ataatttata tgaggtcctg
    12541 ttttatagct ttcaaaaagt tctttatcaa tgtgtacact gcgaattgta gaattcacaa
    12601 cttccatttt tctccccaat aatttaaacc attcaggagc tatatcactg ttgagaatat
    12661 aaaaatcaac atctctattg tgatacaata cagactttat agtcgtctct aaaggaataa
    12721 gataggcatt atctgccgct aatactattg attttctcat actgttctct acctcatgta
    12781 agatctgatt gctgcaacca tttttttcgg aatggagctt ggatagatac cttcatatga
    12841 aatctcagaa agattttcag agcaagtatt gtcgaaagct atcattggtt ttttgtactt
    12901 aagcacaaac tcatatgcat cttctaattt tcgatcatga ttcaaatcca aatacaaatc
    12961 tgaagctatt actatttgtt ccaataacat tggtaaggaa tttggatata cggtaacatt
    13021 aggaaaacgc gttaaagcta gtagttcatt tgccatattt gtataagcaa caatatgaaa
    13081 atgaacttct ggcaattgct ccgccaaata atcgattttc tcgacacacc aagaattcgt
    13141 taaattaaca cattgtaact taaatatttg tgatttacta ggatacttca cactctttga
    13201 aaaccattca tttaaaataa cagaccaatc catcaaagag tattcccacc aaacttctct
    13261 tagacgtcca acagaaaatt gattccaagg cttatcctga gaaatatagt gtaaaattag
    13321 tggcagtggt tcgagcggaa tatcaaaaat gaattgatgt ttaaaggctg ccgccccata
    13381 atcatatcct atttgaaaat tatattgatc ttcaagggaa ctatattgat ctttaaacaa
    13441 catattcaaa attgactggt ctccttcttc cacattctca tgttcttttt ctgttaagtc
    13501 aatcaatttt tgtcgaatag tttcagatcc ccattttttg ttgttaatca agagaacacc
    13561 agcattgaag ccgactcctg ctccaaagca agaacgagct gctgccaaat aattttcacc
    13621 taagtctaat tcaaacaaat cggtcaaatc accagtcaca atcaaatcac tatctagata
    13681 gagaacttta tcttctgtta caaaatctgg aataaaatag cgtgcaaatg tcatatgatt
    13741 gatatgaggt aatttattag accaattcat ttgaaactgt gagccaatta acttgcagtc
    13801 aatcaagtcg ccccccatct cttgtaaata tattcttatt tgactaaacc attcctgagg
    13861 aatgtcctga tttagcagat aaattttcaa atgactattg tgtctacaga gtgacttcat
    13921 cgccgtttcg atttgtcgaa tataagcgta atcacctgca aatgctacag cgcgtttggt
    13981 atttctcatt ttaaatcctt atcatttatt ttaaatagtc tagtttaata ttcgtgcaac
    14041 aatctgactg ttataggttt catcatcaat aaattcaata tcttgatcaa aattatactc
    14101 tgcagtatgt ttatgagaaa atttaacctt agttttagct cccattttca tccattcata
    14161 ctctgagtca atatggccta catccaaaac ttgatagccc atctggcata gattataact
    14221 cagaactttt gctgtaggac ccagcataca taaaatcaag cgaccaccag catacttttc
    14281 aatttcttgt tcaatttcat gaactctaga aaaggcacta tgagaaggac agataattcg
    14341 cttaatagaa tttgcctcat cgaataaatc atttccgaca cctgaacgag aagtcgcacc
    14401 ttcgactatc agtaagtcac ggttttccca aatgcttttc aatttttcaa attgagcttt
    14461 agcttgactc ttgtcttcaa aatcgatata agggcgagat acaaaggttg agccatacca
    14521 tgaatcactg cataactctc tgtaaaaatc catataatga tccatgtgat ctttccaaaa
    14581 tggaatcgcc cacgatgtaa acctaaaacg atctgtaaaa gcatcaggaa ggcacactac
    14641 taaatcttct cgactttctt ggccgataat gtccctcatg attgaaacca actcttcatc
    14701 ataatcctgg tagggaattg aatgccctgc aagcatattg atttccccat ctccaaaacg
    14761 aactaaagaa gagttgtgtt caataatata atccaaggtt tcatctatcc ctttcacctg
    14821 gattatatca agcggttttt ctagcatatc cttagttatc aatttttgca aatcagccaa
    14881 cattcgttca ggatgttcac ttgaatacaa cctttggcca ttattcccat tttgagattt
    14941 ataaaaggct agtataggtt tagatagatg agccatttct gcaacgatat cggatgttga
    15001 agtcagtagg ttgatatcta aataagcatc catcttctcc ttcaactcgt ctagcttatc
    15061 tctagaaatt gccggataga gctgaatatt aggataacga ttagacaaat catataattt
    15121 tggccccata gctgtccaag cagcaacttg aaaaacaaca tttggcaact gtccagccaa
    15181 gtaatccaat tgttcgatat cctgagaagc cgtaaacgtt aaacaagtta aacttggaag
    15241 ttgtcgaaaa caggtaagat gtccgttttt cttaaaatct ttaatcgtat caatcataaa
    15301 ggaaactttc ccattttcga aaacgatttg tcctaactct ccgtgctgag tcgattgaaa
    15361 agctagtagc gttttttctt gttcttgcaa agattttaaa aagttctcat ctgcactacc
    15421 atgattgata tccaaatata gatttgttga ctttttcagc ttatctaata caggaggaac
    15481 aatttgtgga tgcaatctca cattatcata cacagctaat tttatcagct tatcacccat
    15541 atccgtccaa gcagcaatat gaaataccac atctggtaaa gctgtgacta actcttctat
    15601 tccttctaaa tcttgggaat ttgtcaaagt taaacaagaa aattccttat ctaaagacga
    15661 tgtcagttca aactccccta tatgatgttg taaaatttta gtccattcca aatcatgaaa
    15721 ttcccaccat aaatcacgat aacgattggc tatcaaggtg gtccagggtt ttctgtaaga
    15781 cacaaaatga ataatactag gaggtactaa ctcattaaaa taattttccc atttattata
    15841 aaaagcagtt acatcatgac ccacttgaag gttaaattct ttatccagct ctaaccaatc
    15901 atcttgtaag acctgattaa aaatcgtttg atcaccatta aaatgctcga aacggccttc
    15961 ttcaacttcc ttcattgtaa caatgctctg ttcaattagt cgttctttta atttctcttg
    16021 acgccatttt ttattgttga ttaataaaac acctgcatta aatgtaattc catctgtatc
    16081 tttaactgct gcgagtgatt tttcttctaa acaaatacta aataatttct ctaaagaagt
    16141 atttacaatc aaatcactgt ctaaatataa aaccttatct tcttggatat aatctgcaat
    16201 aaaatatcta gcataggtaa tgctactaat gtgatcttgc ttttcccaat cttgaaacac
    16261 agtttgttca ggtagcttca catcgataat ctcactacct aacatgcgag ctatttttcg
    16321 tggtttgcga aaccaatctg gcataatatc ttgatttaaa atataaatct taacatctcg
    16381 attgtggtat aaaatagatt ttatggttgt ttctaactgc ctgatgtaat tacgatcacc
    16441 agctaataca attgttttaa tcatcttgtt ctcttattca actcccaaat ggtaaattgc
    16501 atctaccaat aattttttag taaaataacc ttcttttagc aaataactaa aggtttttat
    16561 ccgattcgtc atttcttgat attcttgtag attcatttta tcaactatcg catgaacttc
    16621 ttctagacta ttcgccacaa agcctaagcc ttgatctact ataaatttag cagttgacaa
    16681 gctacttggt acaatgactg ggatgcccgc tgtcagatag gtactcacct tatgagatat
    16741 atttaaggta taatattggt tgctttctcc atcgttttga taagttcccc agacaaggcc
    16801 aaatccaccc tttgataatt ctagcaacaa ttcctcatct tttttccatc cttcgatgct
    16861 gagatttcta gcactagaac tagcttcccc tttatttgaa aatactctca aaggcgtatc
    16921 ttgagaccaa ttttgtaagt ctggaaaacg ctctaaactt ccagcaaaaa aaagttcttt
    16981 tttaaaagca ggggtgtata aggataaatc atgaggatga tcccacatcc cttgaacaag
    17041 aatcttctta gtcgtcaatc cttcttccat caggcgtgtt ttcattctct ctgacggcac
    17101 tatcaaaaca tctgatagat tatacatgta catatagtct ttcatgagat aatagttact
    17161 atcaaacatg aggggaacaa catcatggat aaagcaaata attttcacct gcatatcctt
    17221 caacttatca aacaagagac gatcaaattc aaagccattc caggttggag actgaaagac
    17281 taaaatatcc ccaatagaaa tactggccat aataccatcc agacgcttat tcatttcaga
    17341 aggactatct gaagcaatgt tgtaaaaata aataccaacc tctctaaatc ccagttgact
    17401 agctatcttt tgaacagcat tttgagctaa gataaccgta ctatcaccag ccatgccgta
    17461 taaatttgtt aaatgtagtt tcatattacg ctatctcatt ttttattact atctacggaa
    17521 gtaaagacaa tttttccatc attcttctat aaatctctgt gaactgcata ttttgttctt
    17581 ctaattttgc tatcactctt tgtaatctat ttttataaat caaaatatga ttggtcaagt
    17641 catagtctga acttgctaaa atagctatct tttcttccct agccccgatt tcatcataca
    17701 tacgcttttc actccatgta ttcgataaag tatcagataa accaacacgg taccagtaaa
    17761 cacaacgatt caaatagact atcctcgaag ctcttagaag taccttccat gtccagtaag
    17821 tatcctctgc aatttttcct ataggaaatg gtaaatcttg tagtaactct ctcttgaata
    17881 acttcaagac agctacagtc cagttcccat ttctgacttc ttcgacacct tccctatcca
    17941 tgattgcttt accttctatc acttccagag aatcatctgg atccgtaaca taagtcatat
    18001 acacatagcg tgtttcatca taagaattat aacgcccgat actaatatct gcgttttcct
    18061 ttttcaaagc accatataat cggtctaaag catcatgttc caaccaatca tcagagtcta
    18121 caaaagtaat gtacgccccc cccgaacatt caatacctag gttacgagct gatgaaagac
    18181 cgccgtttgc tttctcaaaa tatttgaaac gagaatcttt ctctacaaat tcttcacata
    18241 ttttggatga atgatctgga gagccatcat taaccatgat acattcaaaa ttcggataag
    18301 tttgtttttg aatactatcc aaacactgtc gcaaataatt ctcaacgttg tagattggga
    18361 ctacaatact aattagttca ttttccatat actaactttc ctagacttga tttattctat
    18421 ttccctctta atctcttctc tttacaacaa gatttccatt attataaact acactgtttg
    18481 atggaatatc attgcgtatg agacaacctg ctccaataat ggtattatcc ccaatcgtca
    18541 ctccttttaa aatcgtgaca ttacttccta tccagcagtc tctgccaact ctaatcggcg
    18601 ctgtagtcca ctgccacttt tcaatctttt cagcagtata aatatggtcg tgatcataaa
    18661 aacgaacacc ctctcccatc atggtaccag ctccgatttc aattctttcc atacagttaa
    18721 aactacaaga gttgttgata aaaacaccat catgcagtat taacttacca gaagagacat
    18781 gaaaattttc aaaatttctg cagatgacat ttgctcctac aatgaagtga gccttatcat
    18841 cgattttaaa gacattccaa gtcccatctt ggatgatttt ttcttgatga ttggtgccta
    18901 ctctgacttc attcagatag tctctcactc tagaaaccaa tttgtctggt gtacttgata
    18961 gaaaaacttc ctgcccctga tttccatgga ctgtattgtc aaaagcaaag acagattttc
    19021 cagcctgtac aaacttggag acaatagcat ctacttcttg aaagtgattg atatctagta
    19081 aaaagtcaca ggtatcgact agaaagttta gctgttcttc atttgtaatc gttcggagac
    19141 ggacattata gttcgtttct aagctacgga ttttctcgga aaattgaaca ggtgctgcaa
    19201 tataaaaaca gatatttggc aaggattcaa ggagataatc caactcttca atctgatcac
    19261 taacagtcaa aatcagagca tctcctacta aatgattttt cttcatttgc ttatttctct
    19321 caatttctca atcattgctt gaacttggtc aacagcatat acctcctgac ctgcttcata
    19381 ggttttggta ttttcaaagg acaaaatagt ttttccaagt cttgcaaatt gattgataat
    19441 ttcctctgtt ttttcaccat gattaatatc taaaagtact tgactggttt ctaccaattc
    19501 attatcgaca tctaccaaat agtgaattcc gttaaatata gtcacgtttg gataaattgt
    19561 catctgagcc aatcgatcac taactattac tctagctgct atcttaaact gaatatcagg
    19621 caaggattga accaatgtct caatttgttc aatatggtct gaggcagtat agattagaca
    19681 agtgaaaggt tcctttattg gatagatgtg agatctttgt aatggatgta aatgatggtt
    19741 ttgtcccaat tctgtccatt caagcccatg atagtaccac caaacttcac gataggtttg
    19801 ggccgccaaa tctttccacg gtttccgatg agaaagatag tgaataatag caggataatc
    19861 ctgaccctca ggcaattgat aatcagcaaa ctgtttatga atgacaatat gattataatc
    19921 aaagtccaat tccaaccatt tatgttcaaa aagcatattc aagatgctct gatctgcctg
    19981 atccacctta tcatgccatt cattggttac atcaattaat ttttgggtca tattctcttt
    20041 tttccaaaaa gcattgttta ccaagagaac accggcatta aagatttctt gaccaaaata
    20101 agctctgccc ccaaaatctc taacagcagc caaaggataa tcttgtaagt ctgtagcaaa
    20161 caaatcatca agatttttcg ttacaagtaa atcacagtcc aagtagaggg ccttatcttc
    20221 ttgcacgaaa tcagctatga aatagcgtaa aaaaactgtg taactaatat ccgttttata
    20281 acatgaaatt tgctcagaag ttacccgaca attaataatt tctgagtcaa acttctctaa
    20341 gcgcttattt aattgcttaa tccattcatt tggaaaatcg ctatgaatca gataaaaacg
    20401 aatcgaacga ttatgataac aaatagactt gattgtcgtt aaaacttggt ctacatagcc
    20461 atagtttgct gctaggacaa tggctttctt ttcacttgat tcttgtaccg ttaactgatt
    20521 taaaagtctt tttttcattt caaactcttt atatattgct gtgtcagata aaccactagc
    20581 ttgaccgttg gcgagactga cttccaacat ctgacgataa actgccaagt gtctctctag
    20641 aggatatccc atattagcta gtagcgtaat acgttcagac atagcatcaa ctaaggcgtg
    20701 catccacttt tctgtccaaa ctcttgatag actacctttt ctaatccgat aagcataaag
    20761 acctttattt aaataaatta ccttttccga taataaatat accttttgat tgaggtaacc
    20821 atcttctcct aatttaccca tgtcaaagcg caactgctca aacaatcttg ccttatagag
    20881 tttaccacaa gcagatatca aagcaaaact cttcatttct tgagtttcat aacaagttct
    20941 caaagatgga aacattgtca tataccttat cataatagga gtctcctgat atatgaaagt
    21001 agaacattcc ttcactttcg ttgaaagaat agtaattccc aactgcaata tcagcttgat
    21061 attctgttat ttttttatat agggtttcta cataatctaa ctcaatccaa tcatctgaat
    21121 ccacaaaggt cacataattt ccggacatat tattcagacc ggtgtttcgt gcggcagaaa
    21181 taccagcatt ttcttgttct atatagagaa ttcggtgatc catttctgaa aattctttac
    21241 aaatttcacc tgaagcatcc gtagaaccat cattaacgac aacaatctca atatttttat
    21301 atgtttgaga aataatacta tctagacatt tcctcagata gttttctaca ttgtatactg
    21361 gtacaatgac cgtaatttta tcatccacta gcaattttct ccttgattta tgtgcgcgag
    21421 ttagtttagc ctaactttac aggttgtctt actcttcttc atcacgttta cggcgtttcg
    21481 caaccaatcc aatacctgta acagctgcta gaactccaag taacacagat ccaattgacg
    21541 actcagtacc tgtattaggc aattcttttt gggatttacc tgtcgatcca gaagcatttc
    21601 caacttgtga gttcgaatgg tttgctgaat ttgaaactga tgtacttgct gaggctgacg
    21661 cactcgttga tgctgactca gacgcactgg ttgatgccga ttcagacgct gaggtgcttg
    21721 ctgatgctga ggcactggtt gacgctgaag ctgaggcgct agtacttgct gaagccgagg
    21781 cactggttga tgctgaggct gaggcgctag tacttgctga agctgaggca cttgtcgatg
    21841 ctgaggccga agcactggta cttgctgaag ccgaagcact cgttgatgct gaggctgaag
    21901 cactggttga cgctgaagct gaggcactcg ttgaggctga agccgaagca ctcgttgatg
    21961 cggaagctga cgcactggta cttgctgaag ctgaggcgct tgtgcttgct gaggccgagg
    22021 cacttgtcga cgctgaagct gaagcactgg ttgacgctga agctgatgca ctcgttgacg
    22081 ctgaagctga cgcgctggtt gaggctgagg ctgaggcgct agtacttgct gactctgatg
    22141 cactggtact tgctgaggct gacgcacttg tgcttgctga agcggacgca ctcgttgatg
    22201 cggaagctga agcactggta cttgctgact ctgatgcact ggtacttgct gaggctgacg
    22261 cacttgtgct tgctgaagcc gatgcactcg ttgatgcgga agctgatgca cttgttgatg
    22321 cggaagctga tgcactcgtt gatgctgaag ctgaggcgct agtacttgct gaggctgagg
    22381 cactggttga ggccgattca gatgcactgg ttgaggccga ttcagacgct gaggtacttg
    22441 ctgaagctga cgcactggtt gacgctgaag ctgatgcact ggttgacgct gaggccgaag
    22501 cactggtact tgctgaagcc gacgcactgg tgcttgctga agccgaggcg ctagtgcttg
    22561 ctgaagccga cgcactcgtt gatgcggaag ctgacgcact cgttgatgca gattcagacg
    22621 ctgaggtact tgctgaagct gaggcactcg ttgatgcgga agctgacgca ctcgttgatg
    22681 ctgaggctga ggcacttgta cttgctgagg ctgatgcgct ggttgaggcc gattcagaag
    22741 ctgaggtgct tgctgaggct gaggcgcttt gactagcact tactgagagt gacttagact
    22801 gtgaaagtga ctgactggtc gtgctagatt caactacctt tcctctaccc ttgaagtagt
    22861 tagttctaat gttatctgtt ttagcagcgt aaggggtaaa tgtaaaggat gtatccgttc
    22921 cagtaattgg tacagtccaa gatgatgtta atccatatcc ttgcttagca aagaaaccat
    22981 tcatttgggc accatttccc caagtatacc cactacccca cgtcgtcatt gtagatgtat
    23041 tataggatcg aaccagtaca ccatctttgt cagtaatgta gttctttaca tctgaagggg
    23101 taccaagatc actgccaagg gttaacattg tttgtattga agtacctgta ttatagatag
    23161 aatacccatg acccatcttt gaaagattac caagagccct tgtggtagga tctacatacg
    23221 taaccgtata ggtaaaggtc agttttgaac catcatttgt cactgtcaat ttatagtaaa
    23281 ttggaacatg atataccgaa tctaaacctg tacctgtata aacttttgtc tcacttttag
    23341 caatgtttag acttgcatta attgcagggg cgccatttac aatcgtattg ccagaaaaaa
    23401 cagcagcttt tatagaagcc agcaattgct cgatggaatc cactgaacgc ttgcgtcgct
    23461 tagcataaga ttggagattg acatttgtaa ctgatgctgc ataatcacta gctggtttct
    23521 tacgatcttc ttcgaccttc ttagcagttg cttctgtagc ggcagctgtt tgtgatccta
    23581 ccacagtaga tgatgcagag atacttgtcg aagccgatgt acttgctgat tctgatgcac
    23641 ttgtactcgc tgaggtcgaa gcgctggttg aggctgactc agatgcagat gtacttgccg
    23701 actctgaagc tgacaaacta gttgaactcg ctgaattact tgtagatatc gtacctagaa
    23761 ctactgtatc atttgttgcc aaagcatccg ttttctctac agttttttca agtactgccg
    23821 actcatttgt aaataccttc gtttgagttg caacggttcc acttatgaca gttcccgcag
    23881 caactattcc cttgaggata tcaagcccag taattgaatg acttacttta tcttctaccg
    23941 tttcggtcat gacctgagta gtatcaacac caccacgcat gaccttaaac aagccaaaaa
    24001 gagaagtcga ggcccgtagc caatgcttcc ctgatttgat taatttgaac cgagttactc
    24061 ggtctgtttc tctatattca cccttctgac gtctaaaaaa tattttgaaa tttcctttcg
    24121 agtattattt tcatgtatgg atataaataa attaccatac tataattata gaatagggaa
    24181 cccaaaatag caagaaactt gaggtatttt tgctaaaaaa aaaaagcatt ttactatttt
    24241 atatatatat tataagataa gttttttata aagacacgca aatgagtata aatcagctaa
    24301 tttttggttt atattcctat ttttatgcat aattattctt tcattctcta atttgtgaat
    24361 caatatatct gattagaaaa ctaccattga gtttttgctc cacctcatta gtccgctcaa
    24421 aacgccgtcc gctaatcttg tcatagactt tctcccctct aggagcccct gcataaggac
    24481 gatagaggta gcggttgatt cccgtttcgt caatatatag gactggtaaa tcttttaggt
    24541 tatccaaaat atcaagaaac tcagaaactt tctcaggctt ttgttcccta aaactggtcg
    24601 tcttttttaa aatgacctta atctgcttta aaactgccca tacggagggc aaagcacaat
    24661 caaaacgggc cgcaatttcc cgtaaaaaag cgtctggatg agcctctaca aaggctttca
    24721 attcttctaa agggatctta cgctttttga cgactcgctt tttccgctct aagttccctt
    24781 gtttacgtct ttcttttccc acgtgaagag agttctgacg ccaacaccaa aaaacttggc
    24841 tgcctcgaca tgactgtgcc cctctttgat ggaatctaat gctccttgtt taaaatcagt
    24901 actatatgcc atagctttat tagaccatgc ttattttaaa ctaagcaact atagatagag
    24961 aaatgaaata aaatctaaat tcgaatgtga taaggagtta gaaattgaaa caataggatt
    25021 cccccattat tcacacaaaa ataaaaccac taaaaaacag agcttgccag cattcttgaa
    25081 aagtagtata attattgcat gcgcaatcat cttgtgacgc agagactgcc cgttaatgga
    25141 ctttcttcta tattatgact ctaaaaagaa aggagacact atgacctatt tggaaaaatg
    25201 gtttgacttc aatcggcgtc agaaagaaat tgaaagtctc ttggaagaga ccattgccca
    25261 acagagcgag caaagtctga ccttgaaaga gttctacctg ctctactatc tggacttggc
    25321 tgaagaaaaa tctctacgcc agattgacct gccagataaa cttcatctga gcccgagcgc
    25381 agtttctagg atggtggctc gcttggaagc taaaaattgc ggtctactca gtcgcatgtg
    25441 ttgccatcaa gatagacgct ctagttttat ctgcctgaca aatgatgggc aaaagacact
    25501 ggcctcgcta caaaagactg tcgaagaaag cttggaagca ggtttggatt tcttgattta
    25561 agatgatttt aaaaaaagtt gcgtgcgcaa tcatttttct tgacattctc ttttataagg
    25621 agtaaaataa agttagcatt aaacaaagga gttttaaata tgattgaaat tacctatata
    25681 gatgccagca agaacgaaag aactgtgact ttcgagtctt atgaagactt tgaacgttca
    25741 caacaagctt gccttatcgg cgtcgcagac tactaccctg tccaaaaatt aacttacaag
    25801 ggtcataatt tggactacca tgggacttat ggagatatct tcttctatct catgaaacaa
    25861 gatttaagcc aatataacta aaaaaaggag aaatacaatg gcaaaagcaa ttacagatgc
    25921 aacattcgaa caagaaacaa aagacggttt ggtcttagta gacttctggg caacttggtg
    25981 tggtccatgt cgtatgcaag gtccaatctt ggacaaattg tctgaagaac tttcagaaga
    26041 tgtcttgaaa atcgttaaaa tggacgttga tgaaaatcca aacacagctc gtgcttttgg
    26101 aatcatgtct attccaactc ttctcttcaa aaaagacggc caagttgtca aacaagttgc
    26161 aggtgttcac acagcagaac aaatcaaggc catcattgct gaattgagct aatcaaacga
    26221 gagaccaagt actttctttg gtctcttttt tcttgccctt tgccattttt caaaaaatat
    26281 gctagactat agtagattga aactagaata gtacacctct acttctaaaa cattgttaaa
    26341 aatcgatttg actgtcctga tcgatttgtc ctgtttttat ttcattttac tatataagta
    26401 gaatattacg atgtttggga tatgccatcg ctaaaaaaaa actcgatttg gtatttttta
    26461 gttccctaat gggagctttt tgcgtgctct gaacatttcc cattttggaa ggagtactat
    26521 gaaacatcaa tcagccttgg tcgtctttag tggcggtcaa gattcaacaa cctgcctttt
    26581 ctggaccatg caacactatg aaacagtcga agctgtcacc tttgcctacg gccaacatca
    26641 tcacctcgaa attcaaatta ctagagaaat cgctaaggaa cagggcattc gtcaccatat
    26701 cctcgatatg tctctgctgg gacaaatcac tgctcagcca gactttgcga cgattcatat
    26761 ttcctacatt cctgacaagc tctgtgtcga gtcaaaatcc ctcaaactat atctatttag
    26821 ctaccgaaac cacggagatt tccacgaaaa ctgtatcaac accatcggga aagacttggt
    26881 caacttgcta gaccctcgct atttagaagt ctggggaaaa ttcactccgc gcggtggcat
    26941 ttcaatcgac ccctactata actacggtaa gcaaggaact aagtatgagg gcttggcaga
    27001 acaacgcctc ttccaacacg acctttatcc agagaaaatt gacaaccgct aaagtcatac
    27061 tcaatgaaaa tcaaagagca aactaggaag ttagccgcag gttactcaaa gcagtgtttg
    27121 aggttgtaga taaaactgac gaagtcagat caaaacactg ttttgaggtt gtggatagaa
    27181 ctgacgtagt cagtaaccat acctacggca aggtaaagct gacgtggttt gaagagattt
    27241 tcgaagagta tcatacgaaa aagccctgtt cctcaagatg aggagcaagg ctttttgagt
    27301 ttcaattatt cttctgttcc aaggaaattt tttgcaacaa gggctgcaag aactccacca
    27361 gcgattggtg caaggatgaa aatccaaact tgttgaaggg ctgcgccgcc taccaagaca
    27421 gctggtgcca agctacgagc tgggtttact gaaagtccag taatcttcaa tccgacaaga
    27481 atcatcgcca tcaatgacaa accgattacc aaaccagcaa tcgcgccatt gcccttgctt
    27541 tctgaagtca cagtcatgat aaccaataca aacaagaaag ttgcgatgac ttcaaacaag
    27601 aaaccaccaa agacagtgac accgtttgcc aaggcatttt caccaagact agcagttgac
    27661 atacctgaat tactcaagag gaagaagact gcgccagaag cgatgaaagc tccaacaacc
    27721 tgaccaagga tgtagtttac aagttctgaa gatgacaaac gtttgtttac aaacatagca
    27781 atcgaaacag ccgggttcaa gtgagcacct gaaacagttc cgattgagta ggctgccacc
    27841 acgattgcca aaccaaaggc aaaggcgatt ccaaggtgac caaggccatc aagaccattt
    27901 ccaaaaacaa cagctcctgt tccaacgaac acaagcatga acgtaccgat taactcagca
    27961 acaaattttt tcattttctt tctccttttt tcaaaaacta gatactagtc tatcaaaagt
    28021 aggaaagggt ttcaagaaaa ttgattggaa attttttgaa aatcatagaa ctattagcta
    28081 atctctagta ttgaaaagac tggatagctt ctttcaactc atcttgtaaa ctatttctct
    28141 ggtcaagttg gacatagact tccaccagac aggatctaaa gttggaaaat ttgtaaaaat
    28201 cctccctttc ttctatcgga aaatcaacag tttttatcca agaagctact tgttcttgcg
    28261 ctaacttccc ttgtaaaata ggttcataga tcactcttgc taaacgccaa tcctcatcat
    28321 ctgtaaagcg aatcgacatt cttttaaata attggccaag tatctcaaat acttcatgaa
    28381 ctctgttttt aggaaagccc ggatgacaaa ccacctctgt cagtaaatcg gctccatgtg
    28441 caaaagcatg aacccaacca tactgacttg aaaaacccct tgtatccttt tcttttgaaa
    28501 gatagtgcaa gccttgattt aaaaggacat tacgaatttc tggagaagga tttcccaaat
    28561 gatcaaacaa ccactggatt tcttcctggt tataatttgg tttttcttct gctatttttc
    28621 ttagtaaatc ttgatacatg gtcaatacct ctacatttct agcaactgtt caaaaaggca
    28681 gtcttaaatg actcaatatt gaattctcaa ttaaatacaa tctgatataa aatgacgtaa
    28741 ataactatca ataccagttc tacagtaagt tcaaatttaa catcacgatc ttcaacgaca
    28801 tttttgaaaa tagctacaac taagacaaat agaatgacgc ttaacaagcc cataaacatc
    28861 attctaaaaa atttttctat tcccctactc tcccaactca gcactatagg agataatctg
    28921 gtcaactgtg tcagacaaga attggatggt atcacggagt ggtttgtctg ttgaaatatc
    28981 agcaccgata atcatggctg actcaagtgg tgtcttgcta ccacctgatt tgaggagatt
    29041 gagccagtct tcagctccag tttcagaatg ttttagatga aggtaaccag cagtcgagat
    29101 aactagtcct gctgagtaag tgtaactata caagcccata tagtagtgag cttggcgcat
    29161 ccaagtcaga gttgcatcat cgtcaatttc aatagcatct ccccagaaat ccgtcaaaac
    29221 ttccttcata atgctgttga gcttgcttgc tccaaaggtc tccccttctt caatcaatgt
    29281 atacacctta cgctggaagg cggcttccaa gaggtgggtg ataaagttat ggaagtaggt
    29341 gtctgtcaag cgatgagcca gagcgaagcg tttttgacgt gggtcattag actggttctc
    29401 caagtaatca ctgagtagca attcattgaa ggttgacggt gcttcaacat agtaggtcga
    29461 catatgggca ttgaagtaac tttgatgatt gtctgaaaag atgaattgac cagaatgccc
    29521 gatttcatga atcaaggtat agacatcgct caaacggcct gtccagctca tgagtacata
    29581 agggtgtacg cgatatgggt ccgccgcata accaccggaa tccttgccac tgttagcagc
    29641 aaagtccacc cagcgctctt cttggtaacg agcaacttcc tgacaatatt cttgccccaa
    29701 aggttctacc gacttcatga ccaaatcata ggcatcgtca atagtcactt caggattcag
    29761 ggcgctgtcc aagtccaatt tccagtctgc aaaggtcatc ttttcaagac catttacctt
    29821 ggcaacatgc ttgaggtatc tctgagcgac tggtgcaaag tccttcatga tgaggtcaat
    29881 ctggcggtca aacatgacac ggtccacttc ttgttcagct agaagatagt caaagacaga
    29941 gtcgtatccc ttcatatcag ccaagagttt ttcagacttg acctgagcca gataggctgc
    30001 tgcagccgta ttttggtgct tacgaagtcc ctctgagaag gaacggaagg atttctcacg
    30061 aacctcagca tcctcatggt tttggtagaa attctcatag gtcacaaagc tgtttttgta
    30121 ggtcttgcca tgggcttcaa agtcagccat ttcaaaatcc ccagctcgca tcttagtata
    30181 aatgtcctgc ggactgtaga aaacttcacc gagatttgtc aaggccttct ccacatctgc
    30241 ccctaagtag tgggcttttt tgattttagc ctgacgaatg gcagctgtta aatgtggcaa
    30301 tttacccaaa cgatccaaga cttcctcgtc tgctgccacc aaggcatcgt caaaaaaggt
    30361 caaggctacg ctggcatctg tttcaaattc catcccagct tgagcaatat ttgcaaattc
    30421 gtcattgcta tagtccgtcg tctgaggcat aaaaccatag ttgccaatat ggctcatctg
    30481 aatgtagatc tgttccaatt ccgcaaaggc cttctcgaaa tcctcaaaag tgtgaagatt
    30541 gcccttgtaa tcacggctaa actggttgat gtcttcgcga gctttctcga ttgcacgcaa
    30601 gaaatcctca cggtcttggt atagagctgt taaatcccaa agttccttct ctggaaattc
    30661 tgaacggtgt ttttgttcca ttttcttcct cttatttctc taattctact aaaacactaa
    30721 gggctgataa ggcgtaaagc ggtgctgttt ctgctcttag aatgcgaggg ccaagtcctg
    30781 ccaaaacagc tcccttagct tcaaaatttt caatttccgc aggtgacaga ccaccttctg
    30841 gaccaaagat aaagagcaat ttggctcctt tctcgagtcc agtaactgct tttataagag
    30901 cagcggcttc tccttctttg gccgcctctt cataggctac taagatgcgg tcaaactggt
    30961 caagttgggc tagaaagtct gctttattct cgaaaagatg gatacttgga acaacattac
    31021 gcttactttg ctctgctgct ccaagagcaa ttttttctag tttttcaacc tttttaccca
    31081 atttcttgcc atcccactta gcaacggacc aatcggcagg gaaggcccaa atctggctgg
    31141 cacctagttc cgtcaccttt tgagtgataa actccaactt atctcccttg ggaaatccag
    31201 atgcgatggt cacttggact ggtagttcca cattgtcatt taattcttgg accaactcaa
    31261 actgacgatt ttccatatcc agcacgcgcg ccaagcgctt gatgccatca tcaaagacta
    31321 aggtaacctc atcctcttct ttcaagcgca taacctgaaa catatgcttg ctggtttccc
    31381 tgtcctcgat ggtgacagga gagactgcac tccccttgac aaaatactgt tgcatgctag
    31441 cctccaatca cacctgaaat atccttggtc ttcttgaaga cgcaggcatt ccattcccct
    31501 tgaatcatat gggtttcaag gaaaaatcca gctgactcag ccgactggcg caccatgtcc
    31561 cacttgtcct tgataatgcc actcatgatg aggtagcctt catcctttac caagcgataa
    31621 gcatcctccg tcagatgaat gagaatatcc gccaagatat tagccacgat gacatctgct
    31681 tcaatctcaa ctcccttaag caagtctcct gctgctacat ggatattttc cataccagtg
    31741 ttgagctcaa tattttcctg agcgacacga accgccacat catccaagtc ataggcgaaa
    31801 atctccttgg cacctaggag tgagcttgca atagaaagaa cccctgaacc agtccccaca
    31861 tctagcaccg tttcgccacc acgaagaacc tgttccaagg caaaaaggct catcttggta
    31921 gttgggtggg ttccagtacc aaaagccatg ccaggatcca gcttgataat tttttctccc
    31981 gcagtcgcct catagtctgt ccaagaggga acgatggtca aatcatgagt gatgcgagca
    32041 ggttcatagt atttcttcca gttgtctgcc cagtcttcct cagccaaggc agtcgtacct
    32101 atttttaact ctcccaaatc cataaaatcc gtcaattctg ctaagcgagc ctgcaagtcc
    32161 gcctcaacca ctgtcatatc caccgtgtca gggtagtagg ctgtcactac gatttcttct
    32221 tgctgctcca cctctgggaa aatctcgcca aaacgatcca catttcccat atagtccatg
    32281 ctgtcttcaa tcgcgactcc ttgcgctccc agctcaatca agagattgga aaccaactcc
    32341 tctccctcac gcttcactgt aacttttaac tcttgccatg tttccattat taagatacca
    32401 agcccgtaaa acacaaaacc aaaataggaa attctctgaa gacgcttgtg tctaagagaa
    32461 gtttatcttt ttggcacagt gtttagggcg ggttcagttt agaaatgtaa ctgaaccatc
    32521 ctttctaatc acttactttt aaataatctt ttaatctctc ttgcaactga ggcacaactt
    32581 gactggaact aagaaattcc tcaacattca tcagctgata gccctgtcct tcatctccga
    32641 agatgatatt gtcaaattgt tcttgtctta gctgacccac cataaagacc gatttcttgc
    32701 ctttaaaaat tacgctagga taaatcttgc tccaaagcag acagtcttca tctaaatgaa
    32761 ttcccagttc ctcataaact tcacgccgag cgcattcaaa agggctttcg tccccttcac
    32821 ggccaccacc tggcaactcc cacatgttgg gacaagggat atcgtccttg tcatcacgca
    32881 aaatcgtcag tagcttatcc ccacaaaaga gggcaatctt gcaaccttgg aaatcagaaa
    32941 tttctagttc catcttcagt tccttctaac atttcctttt ccagctctgc taacaagttt
    33001 tcataatagc gtttctcgtc cctcaacatt cgactactat ccattttctg tctagcaatc
    33061 ttgagagcct tacgagttcg atctacatct ttcttcacct ttaattgata ccaggcttgt
    33121 atcacttgaa gattggacag tttgagagac agaaacgatt tgacctgtcg aatactagca
    33181 tattgctccg cttgctcaaa atctccttcc aacaaggcga tatgaagcag ggatagttgg
    33241 gcaactgtct gcatcatcgg agtagttgtc ctctcaagta atgcttgaaa ctgctgttta
    33301 gctacttctt ccttcccttc caaaatggaa acttcacctt gcatacctaa tacaccatcc
    33361 gcaaaactcc ctcgtgcatc ctcaggaact gcttgaacaa agtctttcaa atcatattct
    33421 tgaggagcca gcaaagtttg agcagaatgt ctcaatacca ggtaggcgta tttggtattt
    33481 tcagggtgtt gtagtaattc ccaaattttt gctccatcgg tgatgtcgac tggcaaaatg
    33541 ttatttagga agaaagataa attaagaaaa atccaagtcc ctgcaaaata ccagcttctt
    33601 gtcaaaaatc caaacactat cgccaataat atcaagccga gatgaaccat caagcctcct
    33661 gaaagcatca ggatgattct ttgatcgctt tcatcctctt ttaaaccaat gtattgagca
    33721 ccaacatttt tcagaatggc tgttctacta agatgaaacc tgcctgactt tttggtcaaa
    33781 ataaaatgtc ctaatccaaa agccaccagc cgatagcctg tcaagtagcc acaaaaagca
    33841 tgacccagct catgaagaat aaagattaaa tacatgctta gaagagcgaa ggcataacca
    33901 aaagtaaagg ctaaaactgc ggaatacccc aactctgcaa atgcgattgt tccacaagca
    33961 aaagctagca taataaagac aacagctagc acataaacca aataagtccc aattttcttc
    34021 ataacacctc caaccaactc ctagtatctt ggataaggat aaaattctcc ctcttccaag
    34081 ccaacttttc cttcttcaaa gacttcttgg ttccattcca tgacaaattc ctctgcttct
    34141 gggtcttcca aaaagtccat gaggacatct agcccaacct cagcagtatc tttaaggaaa
    34201 agcacaaaat aagctaaaaa ttcacgggaa aatccttttt taggcaggta aggaataaca
    34261 gtcaaatagt cttcctcatt gactgttgac ttagcaggat tgtagaaaag gaccgcttcc
    34321 tcaaaaagaa tgtcatctga tgaaacctct ccgtcttcat ccaccatctc cacacctgca
    34381 gcattttgcg cttccaatag aaaactcact tctaccgcat ggttgcgttt gtcccagcta
    34441 atctcaaagt caaagggaaa gttcttgtcc aactcttcct ctaaaacatc taaaaatccg
    34501 tatgttgcca ttttgtcctc tttctatgcg actctttaat cgccccgatt gctcggaaat
    34561 atgctaaaat agatactacc atcttaccac aaaattattt tatgtcctaa ttataccata
    34621 ttacctcatt taaacccttg gtatcagtga ttttcttaaa agtctgattt cttcatttct
    34681 cataaaaatc aatataaaaa gccctcgaaa gggctaataa atctataaaa tcaataggcg
    34741 agtaactagc acaagtggac gtgctttttt attgactatt accacgatac cacgcttaat
    34801 cttaggcttg aactttctta tctgcaatag cgtctgtcaa agtctgagaa aagttaagtc
    34861 ccatttctcg tcccaactta tctgcccatt ttggtatggt caaagtcttt ttaatgggtt
    34921 cctgacttcc taggtattct gatacatcaa cagataccat agaaataaaa gatttatcaa
    34981 ggtcataggt tgacacgaaa tcttcatcat ctttaaaagg atcattatca attaaagaca
    35041 agctattgat atctgatggc tgaggtaact ctccatcact ctctatcaaa tctgcaacag
    35101 ttatccctag ccactccgac cccatagcca aagcctcaga aatcccctct ccttgtgtag
    35161 ctgagtattc aaaatctggg aaatggacaa aataagtcgc ttctgttccg tctgtgtcgt
    35221 cataataaaa taaagctgga tacgtaacta acatttcact acctccatat caaaaagcag
    35281 ggactgaatt ttacaaccca gcttgctttc ctatccctct ttcagtgtac ttattcagct
    35341 caccatgaag gattgtgata ggtctttccc cttgcttctc gattttaata tgggagcctt
    35401 taccgcctct agtctttatc caaccatggg ccgtaaagag tttaaccatc tctttttgtg
    35461 tcataggcat agcgctttta cctcctgaca acaccattat aacacgtgtt acacgtattg
    35521 taaaggagtg atacttatta ttctattata cataaaagcc cctagatgtg gttctaaggg
    35581 aaaccaattt attcagacct atttttctaa tgagtagtaa aaactgcttc tttatcgagc
    35641 aattcatcat ctgtatagtc aattgtaaaa gtatctcgat ctaagacaga ttgaggcgga
    35701 gttgaatgaa tcataggacc actgcgtact ctatattttt tatctccaat ttttacaaac
    35761 tgatactctt cgaaaatcaa attcaaacca cgtcaacgtc gccttaccgt actcaagtac
    35821 agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtatgat taactctcaa
    35881 gtcttcgaaa tcaggatttt caacagttat tacaaggagg cgatttacta cttcaaaaac
    35941 atcaattatt ctatttttca tattttttca acccattatt agaatgaact tcttggtaag
    36001 caaaatcaag tttagattta atgttttcgt acaaatctaa aatctctttt ggagtatctt
    36061 cccggaagaa aagttttctt ttccctgaaa taacttgatc actaagaatc caatgacgaa
    36121 tttgttttgt aaaaat
//
LOCUS       DAVBCR010000014        35882 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCR010000014 DAVBCR010000000
VERSION     DAVBCR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.014"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(98..739)
                     /gene="plsY"
                     /locus_tag="VQ508_000622"
     CDS             complement(98..739)
                     /gene="plsY"
                     /locus_tag="VQ508_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2489665.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            875..2818
                     /gene="parE"
                     /locus_tag="VQ508_000623"
     CDS             875..2818
                     /gene="parE"
                     /locus_tag="VQ508_000623"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2489666.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3238..5709
                     /gene="parC"
                     /locus_tag="VQ508_000624"
     CDS             3238..5709
                     /gene="parC"
                     /locus_tag="VQ508_000624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2489667.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            5841..6863
                     /locus_tag="VQ508_000625"
     CDS             5841..6863
                     /locus_tag="VQ508_000625"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2489668.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            6919..7623
                     /locus_tag="VQ508_000626"
     CDS             6919..7623
                     /locus_tag="VQ508_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2489669.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7688..8377
                     /locus_tag="VQ508_000627"
     CDS             7688..8377
                     /locus_tag="VQ508_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2489670.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8374..9297
                     /locus_tag="VQ508_000628"
     CDS             8374..9297
                     /locus_tag="VQ508_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2489671.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9312..9956
                     /gene="pcp"
                     /locus_tag="VQ508_000629"
     CDS             9312..9956
                     /gene="pcp"
                     /locus_tag="VQ508_000629"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2489672.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10124..10354
                     /locus_tag="VQ508_000630"
     CDS             10124..10354
                     /locus_tag="VQ508_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2489673.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10404..10484
                     /locus_tag="VQ508_000631"
     tRNA            10404..10484
                     /locus_tag="VQ508_000631"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10438..10440,aa:Tyr,seq:gta)
     gene            10494..10565
                     /locus_tag="VQ508_000632"
     tRNA            10494..10565
                     /locus_tag="VQ508_000632"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10526..10528,aa:Gln,seq:ttg)
     gene            10727..11929
                     /gene="rpsA"
                     /locus_tag="VQ508_000633"
     CDS             10727..11929
                     /gene="rpsA"
                     /locus_tag="VQ508_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2489674.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12015..12512
                     /locus_tag="VQ508_000634"
     CDS             12015..12512
                     /locus_tag="VQ508_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2489675.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12512..14167
                     /gene="dnaX"
                     /locus_tag="VQ508_000635"
     CDS             12512..14167
                     /gene="dnaX"
                     /locus_tag="VQ508_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2489676.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14195..14389
                     /locus_tag="VQ508_000636"
     CDS             14195..14389
                     /locus_tag="VQ508_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2489677.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14539..15309
                     /gene="sufC"
                     /locus_tag="VQ508_000637"
     CDS             14539..15309
                     /gene="sufC"
                     /locus_tag="VQ508_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2489678.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15337..16599
                     /gene="sufD"
                     /locus_tag="VQ508_000638"
     CDS             15337..16599
                     /gene="sufD"
                     /locus_tag="VQ508_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2489679.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16610..17836
                     /locus_tag="VQ508_000639"
     CDS             16610..17836
                     /locus_tag="VQ508_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2489680.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            17823..18263
                     /locus_tag="VQ508_000640"
     CDS             17823..18263
                     /locus_tag="VQ508_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2489681.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18313..19725
                     /gene="sufB"
                     /locus_tag="VQ508_000641"
     CDS             18313..19725
                     /gene="sufB"
                     /locus_tag="VQ508_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2489682.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(19956..21197)
                     /locus_tag="VQ508_000642"
     CDS             complement(19956..21197)
                     /locus_tag="VQ508_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2489683.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21300..22409
                     /locus_tag="VQ508_000643"
     CDS             21300..22409
                     /locus_tag="VQ508_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2489684.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            22943..23290
                     /gene="ssrA"
                     /locus_tag="VQ508_000644"
     tmRNA           22943..23290
                     /gene="ssrA"
                     /locus_tag="VQ508_000644"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23473..23560)
                     /locus_tag="VQ508_000645"
     tRNA            complement(23473..23560)
                     /locus_tag="VQ508_000645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23524..23526),aa:Ser,seq:gga)
     gene            23722..24462
                     /locus_tag="VQ508_000646"
     CDS             23722..24462
                     /locus_tag="VQ508_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2489685.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24459..25370
                     /gene="pfkB"
                     /locus_tag="VQ508_000647"
     CDS             24459..25370
                     /gene="pfkB"
                     /locus_tag="VQ508_000647"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2489686.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25367..27319
                     /locus_tag="VQ508_000648"
     CDS             25367..27319
                     /locus_tag="VQ508_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2489687.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27483..29708
                     /locus_tag="VQ508_000649"
     CDS             27483..29708
                     /locus_tag="VQ508_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2489688.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(29753..30181)
                     /locus_tag="VQ508_000650"
     CDS             complement(29753..30181)
                     /locus_tag="VQ508_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489689.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            30319..31461
                     /locus_tag="VQ508_000651"
     CDS             30319..31461
                     /locus_tag="VQ508_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2489690.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            31470..32684
                     /gene="thiI"
                     /locus_tag="VQ508_000652"
     CDS             31470..32684
                     /gene="thiI"
                     /locus_tag="VQ508_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2489691.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            32819..33643
                     /locus_tag="VQ508_000653"
     CDS             32819..33643
                     /locus_tag="VQ508_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2489692.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            33665..34404
                     /locus_tag="VQ508_000654"
                     /pseudo
     CDS             33665..34404
                     /locus_tag="VQ508_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            34417..35583
                     /locus_tag="VQ508_000655"
     CDS             34417..35583
                     /locus_tag="VQ508_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2489693.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT        10713 a         6589 c         8030 g        10550 t
ORIGIN      
        1 taaaactaaa aggacttagc cctgtgcaat acagaactaa atccttcgga taaattaatt
       61 gtctaacttt tggggtgcag ttcacagaac tggcatttta ttttttagga tcttgatggg
      121 ttaggttcaa tccccaaggg accaaatttt cagttttatt tttgatacga gctatattgt
      181 ccttatgacg aatgataatc aaactagcaa gtgctaagat aatagcgatg aagagagagt
      241 catagttact caggataaaa ccaaaaagtg gaaagagcag aaccccgata acagccgcga
      301 tcgatgctgt gacactagac agtgaaatca tactgccaag atagagagct ccaaagaaga
      361 taatcgcaag gtagagacag aagataggcg caaatccgaa aattactcca gcactggttg
      421 cgacagcctt accaccttta aatcctgcaa agatagggaa ggtatggccg ataacagcca
      481 aaagtccaaa gatgagagga gaaacgcctt gtagatgaaa aataatcgga agcagcgttg
      541 ctagggttcc tttgaaaaag tcaatcacaa aggttgccat accagctttc ttacctaaaa
      601 tgcggaaggt attggtcgtt ccagtgttac cagaaccatg ctcgcgtaga ttgatttgaa
      661 agaatacttg tccaatccag agaccagatg gaatcgaacc cagcagatag gctaggatta
      721 ataaaactat tgtaatcata ctcctattat atcatgaatt gggaaagaaa agcagagaat
      781 ctctgctaaa attgtcacat cggagaaaga aaaattttgc aaaatccttg gaaaacctgt
      841 agaatagtaa agatgaacga ataggaggtt ccttgtgtca aaaaaggaaa tcaatattaa
      901 caattataat gatgatgcta ttcaggtgct agaagggttg gatgcggtcc gaaaacgtcc
      961 agggatgtat attggatcga ccgatggcgc tggtcttcat cacctagttt gggaaatcgt
     1021 tgataatgca gtcgatgaag ccttgtctgg gtttggtgat cgtatcgatg taaccatcaa
     1081 taaagacggt agtctaacgg ttcaagacca tggacgtggg atgccgacag gtatgcacgc
     1141 tatgggaatt ccaactgttg aggttatctt taccattctt catgccggag ggaaattcgg
     1201 tcaaggtggc tataagacat caggtggact tcacggagtg ggttcttccg ttgttaacgc
     1261 cctttctagc tggttagaag ttgaaattac ccgtgatggc gcagtttaca agcaacgttt
     1321 cgaaaatggt ggaaaacctg tcacgacttt gaagaaaatc ggtacagcac ccaagtctaa
     1381 aacaggcacc aaagttactt ttatgcctga cgcgactata ttttctacga cagatttcaa
     1441 gtacaatacc atttcagagc gccttaatga atcagccttt ctcttgaaaa atgtgacctt
     1501 gtctttaacg gacaagcgaa cagatgaagc gattgagttc cactatgaga atggagtaca
     1561 agattttgtt tcttatctca acgaagataa ggaaatcttg acgccagttc tttactttga
     1621 aggggaagac aatggttttc aagtggaagt agccctccag tacaatgacg gattctcaga
     1681 taacattcta tcctttgtca ataacgttcg caccaaggac ggtggaacgc acgagacagg
     1741 actcaagtct gccattacca aggtcatgaa tgactatgca cgtaaaacag gtcttctcaa
     1801 ggaaaaagat aaaaaccttg aaggttcaga ctatcgtgag ggactagcgg ccgttctttc
     1861 tatcttagtt cctgaagaac acttgcagtt tgaaggacag accaaggata aactaggaag
     1921 ccccctagct cgcccagttg tggatggaat agtggctgat aagttgacct ttttccttat
     1981 ggaaaatggg gaattagctt ctaacctcat ccgcaaggct atcaaggccc gtgatgctcg
     2041 tgaagcagca cgtaaggcgc gtgatgagag ccgaaatggg aagaaaaaca agaaagataa
     2101 gggcttgttg tctgggaaat tgaccccagc ccaatctaag aatcctgcta agaatgaact
     2161 ctatctagtt gagggggact ctgccggtgg ttctgccaaa caaggtcgtg accgcaagtt
     2221 ccaggctatt ctacctcttc gtggtaaggt tatcaataca gccaaggcca agatggcgga
     2281 tatcctcaaa aatgaagaga tcaataccat gatttatacc attggtgcgg gtgttggagc
     2341 agacttctct attgaagatg ccaactatga taagatcatt atcatgaccg atgcggatac
     2401 cgacggtgcc catatccaga ccttgctctt gacatttttc taccgttaca tgcgtccgct
     2461 agtcgaggca ggtcatgtct atattgccct cccacctctt tacaagatgt ccaaaggtaa
     2521 aggaaagaaa gaagaagtgg cctacgcttg gacggacgga gaactagaag aactccgtaa
     2581 acagttcggt aaaggcgcta ccctccaacg atacaaagga cttggtgaga tgaatgcgga
     2641 ccagctctgg gaaacaacca tgaacccaga aacacgtacc ctcatccgtg tcacaattga
     2701 agatttagcg cgcgccgaac gccgcgtcaa tgttctcatg ggagataagg tagaaccacg
     2761 ccgtaaatgg attgaagata atgtcaagtt tacgctagaa gaagcgacag tgttttaatg
     2821 aaagaaaata atatgttagt aaactggaaa ttagagtcgg atgtgaatga ctatgtgaaa
     2881 aagcagtttg aaaatcttgg tttgaaaaaa ttgcaggatt attaatgaag aatctgctat
     2941 gagtgcttat ctcaaagagg cgcttaaggg agcggctaag acacaaacta agactaattt
     3001 tggaaaacca gattttcata ttgaaaaata caaacaggat agtttggtta cttatcgtaa
     3061 ctgaacacgg gaggaattcc cgtaaaatat cttttactgc ataaaaacct actctacatt
     3121 ctttgaaagg agttgaacac gccctagata ctgtgtgaaa aagataaatt ctcttgtgag
     3181 tttgcttact tcaagaattt tctattttca cttggtattt tatgggcttt gtatcttatg
     3241 tctaacattc aaaacatgtc cctggaggac atcatgggag agcgctttgg tcgctactcc
     3301 aagtacatta ttcaagaccg ggctttgcca gatattcgtg atgggttgaa gccggttcag
     3361 cgccgtattc tttattctat gaataaggat agcaatactt ttgacaagag ctaccgtaag
     3421 tcggccaagt cagtcgggaa catcatgggg aatttccacc cacacgggga ttcttctatc
     3481 tatgatgcca tggttcgtat gtcacagaac tggaaaaatc gtgagattct agttgaaatg
     3541 cacggtaata acggttctat ggacggagat cctcctgcgg ctatgcgtta tactgaggca
     3601 cgtttgtctg aaattgcagg ttaccttctt caggatatcg agaaaaatac agttcctttt
     3661 gcatggaact ttgacgatac ggagaaagaa ccaacggtct tgccagcagc ctttccaaac
     3721 ctcttggtca atggttcgac tgggatttcg gctggttatg ccacagacat tcctccccat
     3781 aatttagctg aggtcataga tgctgcagtt tacatgattg accacccaac tgcaaagatt
     3841 gataaactca tggaattctt gcctggacca gacttcccta caggggctat tattcagggt
     3901 cgtgatgaaa tcaagaaagc ttatgagact gggaaagggc gcgtggttgt tcgttccaag
     3961 actgaaattg aaaagctaaa aggtggtaag gaacaaatcg ttattactga gattccttat
     4021 gaaatcaata aggccaatct agtcaagaaa atcgatgatg ttcgtgttaa taacaaggta
     4081 gctgggattg ctgaggttcg tgatgagtct gaccgtgatg gtcttcgtat cgctatcgaa
     4141 cttaagaaag acgctaatac tgagcttgtt ctcaactact tatttaagta caccgaccta
     4201 caaatcaact acaactttaa tatggtggcg attgacaatt tcacacctcg tcaggttggg
     4261 attgttccaa tcctgtctag ctacatcgct caccgtcgag aagtgatttt ggcgcgttca
     4321 cgctttgaca aagaaaaggc tgagaaacgt ctccatatcg tcgaaggttt gattcgtgtg
     4381 atttcgattt tggatgaagt cattgctctt atccgtgctt ctgagaataa ggcggacgcc
     4441 aaggaaaacc tcaaagttag ctatgatttt acggaagaac aggctgaggc tatcgtaact
     4501 ttgcaactgt accgtttgac caataccgat gtggttgtct tgcaggaaga agaagcagag
     4561 cttcgtgaga agattgctat gctggcggct attatcggtg atgaaaggac tatgtacaat
     4621 ctcatgaaga aagaacttcg tgaggtcaag aagaaatttg caactcctcg tttgagttct
     4681 ttagaagaca ctgcgaaagc aattgagatt gatacagcta gtcttatcgc tgaggaagat
     4741 acctacgtca gcgtgaccaa ggcaggttac atcaagcgta ccagtccacg ttcctttgcg
     4801 gcttccacct tggaagaaat tggcaagcgt gatgatgacc gtttgatttt tgttcaatct
     4861 gccaagacaa cccagcacct cttgatgttc acaagtcttg gaaatgtcat ctacagacca
     4921 atccatgagt tggcagatat tcgttggaag gacatcggag agcatctgag ccaaaccatc
     4981 acaaactttg aaacgaatga agaaatcctt tatgtggaag tactggatca gtttgacgat
     5041 gcgacaacct actttgcagt gactcgcctt ggtcaaatca aacgggtaga gcgaaaagaa
     5101 ttcactccat ggcggaccta tagatctaag tctgtcaagt atgctaagct caaagacgat
     5161 acagatcaga ttgtagcagt ggctccgatt aaactagatg atgttgtctt ggttagtcaa
     5221 aatggttatg ccctgcgttt caatatcgaa gaggttccgg ttgtcggtgc taaggcagca
     5281 ggtgtcaagg ctatgaattt gaaagaagat gatgtcctcc aatctggctt tatctgtaat
     5341 acttcgtcct tctacctctt gacccagcgt ggaagcttga aacgtgtttc tattgaggaa
     5401 attctagcaa ccagccgtgc caaacgagga ttacaagtct tgcgtgagtt gaaaaacaaa
     5461 ccgcatcgtg tcttcttggc aggagcagtt gcagagcaag gatttgttgg cgatttcttc
     5521 agtacggaag tggatgtgaa cgaccaaact ctgcttgtcc aatccaataa aggaacaatc
     5581 tatgaaagcc gattgcaaga cttgaacttg tcagaacgca ctagcaatgg aagcttcatt
     5641 tctgacacga tttcagatga agaagttttt gacgcttatc ttcaggaagt agttactgaa
     5701 gataaataag tgaaatgaag aatcagttct aagagctggt ttttattgga gaaattttct
     5761 gaaaattaca aaatatactt gctattttag aaaaatagtg tagaatataa gaaataggtt
     5821 tttagaataa ggagtggaat atgacagtaa cgattgattg ggaaaacctc ggtttttcct
     5881 atatgaaatt accttatcgc tatcttgctc atttcaaaaa tggacaatgg gatcaaggag
     5941 agcttacaga ggatgcaact ttgcatattt cagagtcttc tccaagtctt cactatggac
     6001 aacaagcatt tgaaggtttg aaagcttatc gtactaagga tggcagtgtt caactgttcc
     6061 gtcctgatga aaatgctaaa cgtctgcaac gtacatgtga ccgtctcttg atgccacaag
     6121 ttccgacaga catgtttgta gaagcttgta aagcagttgt ccgtgcgaat gaagaatacg
     6181 taccaccata cggaacaggt ggaaccttat atcttcgccc tcttttgatt ggtgtcggag
     6241 atattatcgg ggtaaaaccg gcagaagagt acattttcac catctttgct atgccagttg
     6301 gaaattactt taaaggtggt ttggtcccaa ccaacttctt gattcaggat gagtacgacc
     6361 gtgcagcacc aaatggtaca ggtgcggcta aggttggtgg aaactatgct gcaagtctct
     6421 taccaggaaa aatggccaag tcacgtcatt tctcagatgt tatctatctg gacccatcaa
     6481 ctcatacaaa gattgaagaa gtcggatcag ctaacttctt tggaattaca gctgataatg
     6541 aatttgtaac accattgagt ccatctatct tgccatctat taccaagtat tccttgcttt
     6601 atttggcaga acatcgcttg ggattaactc ctattgaggg tgatgttcca attgataatc
     6661 ttgaccgttt tgtagaggca ggtgcctgtg gtacagcagc ggttatttct ccaattggag
     6721 gtattcaaca tggtgatgat ttccatgtat tctatagtga aacagaagta ggtcctgtga
     6781 cgcgtaaatt atataatgaa ttgacgggta ttcagtttgg cgatattgaa gcgccagaag
     6841 gttggattgt aaaagtagat taaaataaac caaaggagat tttttatgaa atagaaaaag
     6901 tggctcttaa cagcaggagt ggtcctgagc acgtcagcta ttttagtggc ttgtggaaaa
     6961 actgataaag aagcagatgc accgacaaca ttttcttatg tctatgcagt agatccagca
     7021 tcattgggct acagtatagc gactcgaaca tcgaggacag acgttattgg aaatgttatt
     7081 gatggtttga tggaaaatga taaatacggc aatgttgctc cttctcaaaa agactatgat
     7141 ttgaacagta caggatgggc tccaagctat caagatccag cgtcttactt gaatattatg
     7201 gatccaaaat ctggttctga catgaaacac cttggcatta cgaaaggaaa agataaggat
     7261 gttgtagcta aacctggttt ggataaatat aagaaattgt tagaagatgc tgtttctgag
     7321 accactgacc tagagaagag atatgaaaaa tatgccaaag ctcaagcttg gtcgacagat
     7381 agttcattat tgatgccaac agcttcatct ggtggttttc cagttgtaag taacgtagta
     7441 ccattctcaa aaccatactc acaagttggt attaaggggg aaccatatat ctttaaagga
     7501 atgaaattgc aaaaagatat tgttacaaca aaagaatata acgaggtttt taaaaaatgg
     7561 caaaaagaaa aattggaatc caatagcaaa taccaaaaag aactagaaaa atacattaaa
     7621 taaggaatgg tattgatctt gataaaattt ttaaaatact gtcattttga atataaagga
     7681 gtttgatatg gagtggatta gattaatagg aatagcaatc attgttgtgg gttttatttt
     7741 aaaatttgat acaattgcaa cagtagtctt agctggtttg gttacagctt tagtttcagg
     7801 tgtttctctc gttgaatttt tggagatttt gggaaaagaa tttagcaatc agcgagtgct
     7861 cacgattttt atggttacct tgcctcttgt ggggctgtca gaaacctttg gactcaagca
     7921 acgatcaatc gatttgattc gaaagattaa aggtctgaca gttggaaact tctatacagt
     7981 ttatttcttt attcgagagt tagctggttt cttttcaatt cgtctaggag gacaccctca
     8041 gtttgtcaga cctttggttc aacctatggg agaagcagct gcagagtctc aattaggtag
     8101 aaagttaaca gaggttgaag atgagacaat aaaagcgcgt gcggctgcga atgaaaattt
     8161 tggaaatttc tttgctcaaa atacgtttgt aggtgctggg ggagtcctct tgataggggg
     8221 aacattagat cagttaggct atgaaagcaa ttatgcaggg attgcttcta catctattat
     8281 tgttgctgtt ataacactta ttgtagtggg gatttacaat tatttatttg ataaaaaatt
     8341 gatatcaaaa aagactaggg gaggagaaca aaaatgacag agttggcaaa gcaactatta
     8401 gagttgacct atattgtgat tggttgtcaa tttctccata cagcctattg tagttataaa
     8461 gataaaacaa acccagttcg acttgggaca tctgcatttt ggactctatt gtctattacg
     8521 tttataggtg gttcctatat gccaaatatg agtattggta ttattgtaat cctattatcg
     8581 ctgttaacat tgtttaagca agtccgtatc ggaaccttgc catccttaga tgaaatgaaa
     8641 gccaatattg aatctaacag gttgaaaaat aaaattttta ttccagttat gctgatggca
     8701 atacttgcgt tggtcttagc gcaaatgatt ccagaattta gcaagatttc gattagcctt
     8761 gccgccttgt ttgctacaat ttctgttctt gtgattacca atagtcaccc taagagtctg
     8821 ttatcagaaa ataatcgaat gactcagcaa gtttcaacaa gtgggattgt tcctcaatta
     8881 ttaggggctt tgggggctat ttttactgta gcaggtgttg gtgatgttat ctctcatctg
     8941 attagcggta ttgttccttc agatagtcgc tttataggag ttttggctta tgttcttgga
     9001 atggttctat tcacaatgat tatgggaaat gcttttgcag cattcaccgt tattacagca
     9061 ggtgttggag ttccctttgt atttgctctg ggagctaatc caattgtggc tggtgctctt
     9121 gccatgacag caggttattg tgggacctta ttgaccccaa tggctgctaa ttttaacgct
     9181 ctaccagcag cattgatgga tatgaaagat cagaatggcg ttataaaggc tcaagcaggt
     9241 gttgctctag taatgattgt tattcacata ttcttaatgt actttctcgc attttagtaa
     9301 aggaaaataa aatgaaaata ttggttacag gttttaatcc ttttggaggt gaaaagatta
     9361 atccagcttt ggaggctgta aaattattac catctgagat taatggggct gaagttcgct
     9421 gggtagaaat tccaacggtt ttttataagt cgtcagaagt tttagaggca gaaatattac
     9481 gatatcaacc agatgctgta ctttgtattg gacaagcagg cggcaggacc ggcttaacac
     9541 ctgaacgagt ggctattaat caagatgatg ctcgcatacc tgataacgaa ggcaatcaac
     9601 caattgatac accgattcgt attgatggag catcggccta ttttagtagt ttacctatca
     9661 aagcgatggt acaagctatc aaaaaacagg gacttccggc agttgtatcc aatagtgcag
     9721 gaacctttgt ttgcaatcat ttgatgtacc aagctcttta tttagtggat aaaaaattcc
     9781 ctaatatgag agcaggtttc atgcatattc catatatgat ggaacaagta gtaaataagc
     9841 cgaatactgc aggaatgagt ctatgtgata ttgtaagagg catagaggta gctattgaag
     9901 ctattgtaga ctacaaagat aaggatttgc agttagtagg cggtgagact cactgataac
     9961 ctaaaaagga tagtcaatta tgcttgttta ctaactatta actatgctaa atcaattgag
    10021 gttgtttaca taaaactcta tatcagagaa gcctgatata gagttttttc ttgctagttt
    10081 taggattttc ttgtaaaata gaaaaagtga agagaggtat gaaatgagca agaaagataa
    10141 aaaaatcgaa attcaagtag cggatgccaa agttaatgtt ggtaaagaca gttttgaagg
    10201 ttatacattg actatcggta aaaaagttat cggagaaatt gccgaattag acggacaatt
    10261 tgccattata aagaatggga atgtcgatag tttttataaa aaattggaaa aagctgtgga
    10321 aattttgatt gaaaattata atttagcaaa ataagtcttg tttttttgaa attttcatga
    10381 tataatagtc catgttgatt gtaggagaga tagcgaagag gctaaacgcg gcggactgta
    10441 aatccgctcc ttcgggttcg ggggttcgaa tccctctctc tccatttcat taatggggta
    10501 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
    10561 cccagttctt aggtaataat caagataaaa agcagaatat cttagggtat tttattttta
    10621 taattgaaag acgtgaatga tatgaacatg tccttgcggg tgcttaggaa aaaaattata
    10681 agtatgtcaa gtttaagaaa aacttgattg ttggaggatt ttttagatga acgaatttga
    10741 agatttgcta aatagcgtta gtcaagttga gactggtgat gttgttagtg ctgaagtatt
    10801 gacagttgat gcgactcaag ctaacgttgc aatctctgga actggtgttg aaggtgtctt
    10861 gactcttcgc gaattgacaa acgatcgtga tgcagatatc aatgactttg ttaaagtagg
    10921 agaagtattg gatgttcttg tacttcgtca agtagttggt aaagatactg atacagttac
    10981 ataccttgta tctaaaaaac gccttgaagc tcgcaaagca tgggacaaac ttgttggtcg
    11041 cgaagaagaa gttgttactg ttaaaggaac gcgtgccgtt aaaggtggac tttcagtaga
    11101 atttgaaggt gttcgtggat ttatcccagc ttcaatgttg gatactcgtt tcgtacgtaa
    11161 cgctgagcgt tttgtaggtc aagaatttga tactaaaatc aaagaagtta acgctaaaga
    11221 aaaccgcttc atcctttcac gtcgtgaagt tgttgaagca gctactgcag cagctcgcgc
    11281 tgaagtattc ggtaaattgg ctgttggtga tgttgtaact ggtaaagttg ctcgtatcac
    11341 aagcttcggc gctttcatcg accttggtgg tgttgacgga ttggttcact tgactgaatt
    11401 gtcacatgaa cgtaacgtat caccaaaatc agttgtaact gttggtgaag aaattgaagt
    11461 gaaaatcctt gatcttaacg aagaagaagg acgtgtatca ctttcactta aagcaacagt
    11521 accaggacca tgggatggcg ttgagcaaaa attggctaaa ggtgatgtag tagaaggaac
    11581 agttaaacgt ttgactgact tcggtgcatt tgttgaagta ttgccaggta tcgatggact
    11641 tgttcacgta tcacaaattt cacacaaacg gattgaaaat ccaaaagaag ctcttaaagt
    11701 tggtcaagaa gttcaagtta aagttcttga agttaacgca gatgcagaac gcgtgtcact
    11761 ttctattaaa gctcttgaag aacgtccagc ccaagaagaa ggacaaaaag aagaaaaacg
    11821 tgctgctcgt ccacgtcgtc caagacgtca agaaaagcgt gatttcgaac ttccagaaac
    11881 acaaacagga ttttcaatgg ctgatttgtt tggtgatatc gaactttaat caaattgaaa
    11941 attcacaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
    12001 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
    12061 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
    12121 ctcttataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
    12181 aggaattggt tttaggtccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
    12241 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
    12301 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
    12361 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
    12421 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
    12481 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
    12541 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
    12601 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
    12661 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
    12721 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
    12781 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
    12841 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
    12901 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
    12961 ctttattttg gccactactg aattacacaa gattcctgct actattctat cccgtgtgca
    13021 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
    13081 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca gacgggcgga
    13141 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
    13201 tgagctgacg actgctatct ctgaagaaat tactggcact attagcctat cagccttgga
    13261 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
    13321 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
    13381 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
    13441 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
    13501 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
    13561 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
    13621 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
    13681 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
    13741 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
    13801 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
    13861 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
    13921 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggcaa
    13981 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
    14041 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
    14101 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
    14161 agactaaaga aagatttcgt gatacaataa gtttatgaat aaacaacaat ttattattat
    14221 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
    14281 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
    14341 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
    14401 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
    14461 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
    14521 aaaaagaaag gaactatcat gtcagtatta gagatcaaag atcttcacgt tgagattgaa
    14581 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
    14641 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
    14701 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
    14761 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
    14821 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga tgatgagaag
    14881 atttcagttc gtgagtttat tactaggcta gatgaaaaaa tggaattgct caacatgaaa
    14941 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
    15001 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
    15061 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
    15121 ggttttggtg ctatgatcat cactcactac caacgtcttt tgaactatat cacacctgat
    15181 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
    15241 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
    15301 gaattgtaat ttcctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
    15361 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
    15421 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
    15481 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
    15541 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
    15601 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
    15661 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
    15721 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa cgtagaaatc
    15781 acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag
    15841 catattatga ttatcgctgg taaaaattct aagattaatt atctggagcg tttagagtca
    15901 cgcggtgaag gaagtgccaa agcaactgct aatatcacag tagaagtgat tgcacgttca
    15961 ggtgcgcaag tcaagtttgc tgctatcgac cgtctaggtg aaaacgtcac tgcctacatt
    16021 agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac
    16081 gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag ctatgctgac
    16141 ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact
    16201 aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca
    16261 actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcccaa
    16321 caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa cccaattctt
    16381 ttgattgatg aaaatgatgt cactgcagga cacgcagcgt ctatcggaca agttgatcca
    16441 gaagacatgt attacctcat gagtcgtgga ttggataagg caactgcaga gcgtttggtt
    16501 gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa
    16561 atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt
    16621 agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt
    16681 ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag
    16741 ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc
    16801 gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac
    16861 aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc
    16921 tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac accattctaa
    16981 tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct
    17041 taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt
    17101 tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac
    17161 tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat ctacacctca
    17221 tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat
    17281 ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc
    17341 tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatccg ctagttggaa
    17401 ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc
    17461 gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga
    17521 attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc
    17581 tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca
    17641 cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc
    17701 gcaacccttg cttcagtatt tggaagtccc agcatcagct cgtgcaagtt tttatatcta
    17761 taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt
    17821 caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa
    17881 atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac aatccgactt
    17941 gtggggatgt catcaacctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg
    18001 cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg
    18061 ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc
    18121 aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg tcaggtgttg
    18181 ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa
    18241 ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga
    18301 agaaaggata ttatggctga agaaagagta gaaccaaaac caattgacct tggtgaatat
    18361 aaatttggtt tccatgacga tgtagagcct gtcttatcga caggaaaagg actcaacgaa
    18421 ggtgttattc gtgaattatc tgctgctaag ggtgagcctg agtggatgtt ggagttccgt
    18481 ttgaagtctt atgaaacctt caaaaaaatg cccatgcaaa cttggggagc agacttgtca
    18541 gagattgact ttgatgactt aatctactac caaaaaccat ctgacaaacc agcccgttct
    18601 tgggatgatg tacctgaaaa gattaaagaa acctttgaac gtatcgggat tccagaagct
    18661 gaacgtgctt atttagcagg ggcttctgcc cagtacgagt cagaagtggt ttaccacaac
    18721 atgaaggaag agttccaaaa attaggtatt atctttacag atacagattc cgcactcaag
    18781 gaatacccag acttatttaa acaatacttt gcgaagttgg taccgccgac agataacaag
    18841 ttggcagccc tcaactcagc agtatggtcg ggtggaactt ttatctacgt gccaaaaggt
    18901 gtcaaggtag atattccact tcaaacttat ttccgtatca ataacgaaaa tataggtcag
    18961 ttcgaacgta ccttgattat cgttgatgag ggagcaagcg tccactacgt agaaggatgt
    19021 acagcaccaa catattcaag caatagcttg cacgctgcca ttgtagaaat ttttgctttg
    19081 gacggagctt atatgcgtta tacaactatc caaaactggt ctgataacgt ctataacttg
    19141 gtaacaaagc gtgctaaggc tcaaaaggat gccactgttg agtggattga tggaaacttg
    19201 ggtgccaaaa cgactatgaa atatccatct gtttatcttg atggagaagg agcgcgtggt
    19261 accatgctct ctatcgcctt tgctaatgca gggcaacacc aagacacggg tgctaagatg
    19321 attcacaatg ctccacatac cagctcgtct attgtgtcta aatccatcgc taaaggtgga
    19381 ggaaaggttg actaccgtgg acaagtcacc tttaacaaga actctaagaa atctgtttcc
    19441 cacattgaat gtgataccat tatcatggat gacttgtcag catcagatac tattccattt
    19501 aatgaaattc acaactcgca agtggctttg gaacacgaag ccaaagtatc taagatttca
    19561 gaagagcaat tgtattatct catgagccgt ggattgtcag aatctgaggc aactgaaatg
    19621 attgtcatgg gatttgtaga accctttaca aaagaacttc caatggaata cgcagttgag
    19681 ctgaaccgct tgattagcta tgaaatggag ggatcagttg gataaaattt gattttatac
    19741 tcttcgaaaa tctcttcaaa ccacgtcagc atcgccttac cgtatgtatg gttactgact
    19801 tcgtcagttt catctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
    19861 gtttattctt tgattttgag tattagattt actcaaaatc aaggattttg aagatgaact
    19921 tgtatcaaaa aatcgcggtt taaaatcgcg attttttata atttctcgtt aacaaagcgg
    19981 acaaactgat tccaccaaac ttttaagaag aaggcttttt caattttctt gtctgctacc
    20041 atttcgaaac tagggcgctc tgtggtgatg taaccttgac caatcaagtc cttgtcttca
    20101 taagtcaaat ggccaaccac tgttccagct tcaagtggtg ctgggattgc tttggaatca
    20161 ggtgtgaatt gaacagattg ggaagattga ttcccaacac gttcgattag atagatatcc
    20221 tctggagcca ctgcagttac tgtatcttct tttccatctt gtacaggggc tttgctatct
    20281 tgataggcat cgccttgttg aacgattttg cgaagtgtaa atgtagaaga aatataatcc
    20341 attagggaag atgtagctgt aaatcgagcg taaggattat tgtcttgatg atctgcattt
    20401 aaaacaactg tgatgactct catgcctttt tcgacagtag taccaacaaa agactctcca
    20461 gccttatctg ttgttcctgt ttttagccca tcaaaaccac cacggtaagc aggcatacct
    20521 tctaacatgt agttggttga agtgattgtc atcccagcaa aagtagaaga aggttttttg
    20581 gtgatttcta agacttgtgg gtattttttg atgaggttgc gagcaacgat agcgacatca
    20641 taagcactaa gcttattttc ctcatctttt ttagaacctg ggtaaatgtt atcccctaga
    20701 gtttcattgt taagacctgt cgtattgaca acagtggcat cctgaattcc ccattccaag
    20761 agttttgccc gcatcatatc gatgaaatct ttttctgagc cagcaatttt ctcagctagg
    20821 gcaatagcgg cgctgttggc actagatacc agagttgctt caagcaactc ttcgacagta
    20881 taattacggg cctccatagg aatattactg gcttcagaat ttgtcgtcaa ttgataagga
    20941 taatcagaaa tatctacagg agtggagagg gtaatacttc cgttttccaa agcttcatag
    21001 accagataag cagtaatcaa ttttgttatg gaagcaattt cgacaggttg cgttgcatcc
    21061 ttctcataga gaattttacc agtatttgcc tcaacagcaa tcgcatgttt agcggcaatg
    21121 gtaaaatctt gagcaacagc agtagaagca ccccctaaaa gagagacagt taacaaagtt
    21181 aaaaatattt ttttcatagt agtcttattc tatcataaag aaaaaaaata ttcttgcttt
    21241 aataattcat ctgttaagct ttttgaaaat atggtaaaat aaagtgaggg aggtaactca
    21301 tgtttcgtag aaataaatta tttttttgga ccacagaaat tttactctta accatcatct
    21361 tttacctatg gagacagatg gggtctttga ttaacccttt tgttagcgtg cttaatacaa
    21421 ttatgattcc atttttatta gggggctttc tttattattt gacaaaccct attgttactt
    21481 tcttaaataa agtctgtaaa ctcaatcgtt tgcttggtat tttaattacc ttgtgtactt
    21541 tggtctgggg aatggtcata ggtgttgtct atctcttacc tattttgatt aatcagttat
    21601 ctagtttgat tatatctagt caaactattt atagtcgagt acaagactta atcatagact
    21661 tatctaatta tcctgcgctc cagaatttgg atgtagaagc tacaattcag cagttaaact
    21721 tatcctatgt tgatattctt caaaatatcc taaatagcgt atcaaatagt gtggggagcg
    21781 tcttgtcagc tcttatcagt actgttttga ttttgattat gactccagtt tttttggttt
    21841 atttcttatt agatggacat aaattcttgc ccatgcttga aagaacgatt ctaaagaggg
    21901 atcgcttgca tattgcaggc ttattaaaga atttaaatgc gacgattgct cgctatatta
    21961 gtggagtttc gattgacgca atcattatag gttgtttggc ttatattggc tatagtatta
    22021 ttggtttaaa atatgcttta gtttttgcca ttttttctgg tgtagccaat ttaattcctt
    22081 atgtggggcc aagtattggt ttgattccta tgatcatcgc aaatatattc actgatcccc
    22141 atagactgct gattgcagtg atttatatgc ttgttgttca gcaggtagat ggcaatatct
    22201 tatatcctcg aatcgtagga agtgttatga aggttcatcc aatcacgatt ttagttttac
    22261 ttttgttgtc aagcaatatc tatggtgtag ttggaatgat tgtcgcagtg ccaacctatt
    22321 ctatcttgaa agaaatttct aagttcttat cccgtttgta tgaaaatcat aaaataatga
    22381 aagaacgaga aagagaatta gctaagtaaa agtcaggaga accctgattt ttctttactg
    22441 gaagtggcct ttagattaga agactgaaaa taagttaaag tcttaaacta attttcacag
    22501 ctaagaatag tagaagttaa tctgataaaa atcgaaaaaa ccagtggaat tctgtgtcag
    22561 ggtaagttcc actggttttc atagtctatt aaagttcgaa tgaaacctat ttatagtaga
    22621 ttgaaactag aatagtacac ctctaattct aaaacattgt tagaaatcga tttgactgtc
    22681 ctgatctatt cgttctattc ttatttcatt ttactatatt ttggtgcaat aagtgaaaag
    22741 tagtccgaat aatataagga ttgattttat agtttttaaa ctcaaatgaa ttgaaataaa
    22801 gagagtacga aaattctcat ctgaaagtat tttagaataa ttctcttcgt gaatttcttc
    22861 aaaacagata gcttcatctt aggtatgtga tttctttttg catttttgag ttagataagg
    22921 tataatggtt ttattgtctt ttggggtcgt tacggattcg acaggcatta tgaggcatat
    22981 tttgcgactc gtgtggcgac gtaaacgctc agttaaatat aactgcaaaa aataacactt
    23041 cttacgctct agctgcctaa aaaccagcag gcgtgacccg atttggattg ctcgtgttca
    23101 atgacaggtc ttattattag cgagatacga ttaagccttg tctagcggtt tgataagaga
    23161 ttgatagact cgcagtttct agacttgagt tatgtgtcga ggggctgtta aaataataca
    23221 taacctatgg ttgtagacaa atatgttggc aggtgtttgg acgtgggttc gactcccacc
    23281 ggctccatta ttcctttgca ttcttttgca ttccttggta aaacgttgtt aaatcaacgt
    23341 tttttatttt tatctttggt attcctttgc attcttttgc taaaaaggga gtcacaaaca
    23401 gaccctattt taaaaaaagg atagaaaaaa ggatacaaca tttgtcgcat cctaaaaata
    23461 atcttttttc cacggaagac atgggattcg aacccacgca cgctattaca cgcctaccgc
    23521 gtttccaaca cggcctctta agcctcttga gtaatcttcc aatacttact caaatagtct
    23581 accataaagg ctcttatctt gcaataaaaa ttctagaaat aagaaaaatg atagattttg
    23641 aaagaaaatg ataaaaaatg cttgacttca aaagaaagta tgatagaatg aatagtgtaa
    23701 acgataacag gaggtgattc ggtgttaaaa acagagcgga agcaactgat tttagaggag
    23761 ttaaatcaac atcatgtagt ttctctagaa aaattagtta gtttgctaga aacgtcagaa
    23821 tcaacggttc gaagagactt ggatgagttg gaagcggaaa acaagcttcg tcgtgtgcat
    23881 ggtggagcag aactccccca ctccttacag gaagaagaaa ccattcaaga aaaatctgtc
    23941 aaaaaccttc aagaaaagaa gttgctggct cagaaagcag cctctctcat taaagaaaaa
    24001 gatgtcatct ttatcgatgc tggaacaaca actgcttttt tgattcatga attggtcaat
    24061 aagaatatta cagttgtgac caactccatt caccatgccg ctcagttggt tgaaaagcag
    24121 attccaactg tcatggttgg aggaaacgtc aagacggcga cagatgctag tatcggtggc
    24181 gttgctctta accagattaa ccaattgcac tttgaccgtg cctttatcgg aatgaatggt
    24241 gttgacgatg gctattatac gactcctgat atggaggagg gagctgtgaa aagagctatt
    24301 ttggagaatg ccaagcagac ctacgtcttg gtggattcgt caaaaattgg acaaacttgc
    24361 tttgccaagg tagcaccact caaacgcgct atcgttatca ctagtcaagg gcatgagctc
    24421 ttgcaggtta ttaaggagaa aacggaggta atagaagtat gatttataca gtcacactca
    24481 atccatccat tgactatatc gttcgtttgg accaagtcaa agttggtagt gtaaatcgta
    24541 tggacagtga tgataagttt gctggtggga aaggaatcaa tgtcagccgt gtcttgaaac
    24601 gtttgaatat accaaataca gcgacgggat ttatcggtgg ctttactggt aaatttatca
    24661 cagatacttt agcagaggaa gaaatcgaga cacgttttgt ccaggtggca gaagatactc
    24721 gtatcaatgt taaaatcaaa gcagaccaag aaacagaaat caacggaacg ggtccaactg
    24781 ttgaatcggt tcagctagaa gaattgaaag ctattttatc tagtctgaca gcagaagata
    24841 cagttgtctt tgcaggttca agtgctaaaa atctaggcaa tgttatctat aaggatttga
    24901 tttccttgac gcgccagact ggtgcgcaag tggtctgtga ctttgaagga cagaccttaa
    24961 ttgatagttt ggactaccag cctcttcttg taaaaccaaa caatcatgaa cttggagcga
    25021 tttttggggt taaactcgaa agtttagatg aaattgagaa atacgctcgt gagttactgg
    25081 ctaagggtgc tcaaaatgtt attatctcta tggctggtga tggtgccctt cttgtcacat
    25141 ctgagggagc ttacttcgct aaaccaatca aaggaacagt caaaaattca gttggagctg
    25201 gtgattctat ggttgctgga ttcacaggtg aatttgtcaa atcaaaagac gtagtagaag
    25261 ccttcaaatg gggagtggct tgcggaacgg caactacctt ctcagatgac ttggcaacgg
    25321 cggaatttat taaagaaaca tatggaaaag ttgaggtaga aaaacgatga aaattcaaga
    25381 cctattgaga aaagatgtca tgttgctaga tttgcaggca actgaaaaaa cagctgtcat
    25441 cgacgagatg attaaaaatt tgacagacca cggttatgta acagattttg aaacatttaa
    25501 agaaggaatt ttggcgcgtg aagctttgac ttctactggt ttgggtgatg gaatcgcaat
    25561 gcctcacagc aaaaacgctg ctgtcaaaga agcgacagtt ctatttgcta agtcaaataa
    25621 gggtgttgac tacgagagct tggatggaca agcaactgac ctcttcttca tgattgcagc
    25681 tccagaaggt gccaatgata ctcacttggc agccttggca gaattgtctc aatacttgat
    25741 gaaagacggt tttgcagaca aacttcgtca agcaacatct gcagaccaag ttatcgaact
    25801 ttttgaccaa gcttcagaaa aaactgagga acttgttcaa gcacctgcta atgactctgg
    25861 tgactttatc gtagctgtta cagcttgtac aacaggtatt gcccacactt acatggccca
    25921 agaagccctt caaaaagtag ctgctgaaat gggggttggt atcaaggtcg aaaccaacgg
    25981 tgctagcggt gttggaaatc aactaactgc agaagatatc cgtaaggcta aagctattat
    26041 cattgcagca gacaaggccg ttgaaatgga tcgatttgat ggaaaaccat tgatcaatcg
    26101 tccagttgct gacggtatcc gtaagacaga agagctaatt aacttggctc tttcaggaga
    26161 tactgaagtc taccgtgccg ctaatggtgc caaagctgca acagcctcta acgaaaaaca
    26221 aagccttggt ggtgccttgt acaaacactt gatgagtggt gtatctcaaa tgttaccatt
    26281 cgttatcggt ggtggtatca tgattgccct tgccttcttg attgacggtg ctttgggtgt
    26341 tccaaatgaa aaccttggca atcttggttc ttaccatgag ttagcttcta tgttcatgaa
    26401 aattggtgga gctgcctttg gtttgatgct tccagtcttt gcgggttatg ttgcctactc
    26461 tattgctgaa aaaccgggtt tggtagcagg tttcgtggct ggtgctattg ccaaagaagg
    26521 ttttgccttt ggtaaaattc cttatgccgc aggtggtgaa gcaacttcaa ctcttgcagg
    26581 tgtctcatct ggtttcctag gtgcccttgt tggtggattt atcgcaggtg ccttggttct
    26641 tgccatcaag aaatacgtta aagttcctcg ttcactcgaa ggtgctaaat caatccttct
    26701 attgccactt cttggaacaa tcttgacagg atttgttatg ctagctgtga atatcccaat
    26761 ggctgcaatc aacactgcta tgaatgactt cctaggcggt cttggaggag gttcagctgt
    26821 ccttcttggt atcgtccttg gtggaatgat ggctgttgac atgggtggac cagttaataa
    26881 agcagcttat gtctttggta caggtacgct tgcagcaact gtttcttcag gtggttctgt
    26941 agccatggca gcagttatgg ctggaggaat ggtgccacca cttgcaatct ttgtcgcaac
    27001 tcttcttttc aaagataaat ttactaagga agaacgtaac tctggtttga caaacatcat
    27061 catgggcttg tcatttatca ctgagggagc gattccattt ggtgccgctg acccagctcg
    27121 tgcgattcca agcttcatcc ttggttcagc agtagcaggt ggactcgttg gtcttactgg
    27181 tatcaaactc atggcgccac acggaggaat cttcgttatc gcccttactt caaatgctct
    27241 cctttacctc gtttctgtct tggtaggagc aatcgtaagt ggtgtggttt atggttacct
    27301 acgcaaacca caagcataaa aaatagaaaa atgaaaagat tggaccgttt ggtgcagtct
    27361 ttttctcttc ccgaaatgcc tgtgaaatat ggtataatag aagaatggca aacaagaata
    27421 caagtacaac aagacggaga ccgtctaaag cagaactgga aagaaaagaa gcgattcaac
    27481 gaatgttgat ttcgttagga attgcgattt tattgatttt cgcagccttc aaattagggg
    27541 ctgcaggtat aaccctttat aatttaattc gcttgctagt gggtagccta gcttatctgg
    27601 cgatattcgg cctattaatc tatctcttct ttttcaagtg gatacgaaaa caggaaggac
    27661 tcttatctgg ctttttcacc atatttgctg gcttactctt gatttttgag gcctacttgg
    27721 tttggaaata tggtttggac aagtccgttc taaaagggac catggctcag gttgtgacag
    27781 atctgactgg ttttcgaacg actagctttg ctggaggggg cttgatcggg gtcgctcttt
    27841 atattccaac agcctttctc ttttcaaata tcggaactta ctttattggt tctatcttga
    27901 ttttagtggg ttctctccta gtcagccctt ggtctgttta cgatattgct gaatttttca
    27961 gtagaggctt tgccaaatgg tgggaagggc acgagcgtcg aaaagaggaa cgctttgtca
    28021 aacaagaaga aaaagctcgc caaaaggctg agaaagaggc tagattagaa caagaagaga
    28081 ctgaaaaagc cttactcgat ttgcctcctg ttgatatgga aacgggtgaa attctgacag
    28141 aggaagctgt tcaaaatctt ccacctattc cagaagaaaa gtgggtggaa ccagaaatca
    28201 tcctgcctca agctgaactt aaattccctg aacaggaaga tgactcagat gacgaagatg
    28261 ttcaggtcga tttttcagcc aaagaagccc ttgaatacaa acttccaagc ttacaactct
    28321 ttgcaccaga taaaccaaaa gatcagtcta aagagaagaa aattgtcaga gaaaatatca
    28381 aaatcttaga agcaaccttt gctagctttg gtattaaggt aacagttgaa cgggccgaaa
    28441 ttgggccatc agtgaccaag tatgaagtca agccggctgt tggtgtaagg gtcaaccgca
    28501 tttccaatct atcagatgac ctcgctctag ccttggctgc caaagatgtc cggattgaag
    28561 caccaatccc tgggaaatcc ctaatcggaa ttgaagtgcc caactccgat attgccactg
    28621 tatctttccg agaactatgg gaacaatcgc aaacgaaagc agaaaatttc ttggaaattc
    28681 ctttagggaa ggctgttaat ggaaccgcaa gagcttttga cctttctaaa atgccccact
    28741 tgctagttgc aggttcaacg ggttcaggga agtcagtagc agttaacggc attattgcta
    28801 gcattctcat gaaggcgaga ccagatcaag ttaaatttat gatggtcgat cccaagatgg
    28861 ttgagttatc tgtttacaat gatattcccc acctcttgat tccagtcgtg accaatccac
    28921 gcaaagccag caaggctctg caaaaggttg tggatgaaat ggaaaaccgt tatgaactct
    28981 ttgccaaggt gggagttcgg aatattgcag gttttaatgc caaggtagaa gagttcaatt
    29041 cccagtctga gtacaagcaa attccgctac cattcattgt cgtgattgtg gatgagttgg
    29101 ctgacctcat gatggtggcc agcaaggaag tggaagatgc tatcatccgt cttgggcaga
    29161 aggcgcgtgc tgcaggtatc cacatgattc ttgcaactca gcgtccatct gttgatgtca
    29221 tctctggttt gattaaggcc aatgttccat ctcgtgtagc atttgcggtt tcatcaggaa
    29281 cagactcccg tacgattttg gatgaaaatg gagcagaaaa acttcttggt cgaggagaca
    29341 tgctctttaa accgattgat gaaaatcatc cagttcgtct ccaaggctcc tttatctcgg
    29401 atgacgatgt tgagcgcatt gtgaacttca tcaagactca ggcagatgca gactacgatg
    29461 agagttttga tccaggtgag gtttctgaaa atgaaggaga attttcggat ggagatgctg
    29521 gtggtgatcc gctttttgaa gaagctaagt ctttggttat cgaaacacag aaagccagtg
    29581 cgtctatgat tcagcgtcgt ttatcagttg gatttaaccg tgcgacccgt ctcatggaag
    29641 aactggagat agcaggtgtc atcggtccag ctgaaggtac caaacctcga aaagtgttac
    29701 aacaataaaa aaatagcttc tttccaagtt tggagggaag ctattttagt ggctattgat
    29761 tgcttttatt ttctgaagtt ggcgcattgg actgtttttc gttttcagta gcaggtttac
    29821 ttgaagcagg agtagaagag tcctgagttg ctgttttctg atcttctttt ttctcttcct
    29881 tgacgctaga ttttggtgtt tcctcttgct gtgttttttc ttgactagtg ttagtctctt
    29941 tagttggact ggtgttttcc ttaggggatt ccttttggat ttctttgaca atggttgtcg
    30001 tctggcttgt cgtaggttct tttttaatat ttttgttatt atccaaggcg ttcataatcg
    30061 tgatacttgc ggtaatcaag ccaaggatac tagcgattgt agcaacggtt ttttgaaaga
    30121 ctgtaaagcc ttttttgatg tgttctgttt ttggttttga agttctacga tagttagaca
    30181 tacattctct cctttgatta tgatttatta tactctgttt ctttaaaaaa atcttaaaaa
    30241 aagagaaatt tccctttctt gtcgcaagct ccgtttcatg atacaataga ggaagtgatt
    30301 ttagaaagcg tgagaaacat gatttacttt gataattcag cgacgaccaa gccttatcct
    30361 gaagcccttg aaacctatat gcaggtcgct tcaaaaattt taggaaaccc atctagtctc
    30421 catcgtttgg gagaccaagc aacacgaatc ttagatgctt ctcgccaaca gattgcagat
    30481 ttaatcggta agaaaagcga tgaaatcttc tttacctcgg gtggaacaga aggggataac
    30541 tggcttatca agggtgtggc ctttgaaaaa gctcagtttg gcaagcacat cattgtttca
    30601 gccattgaac atccagcagt caaagagtca gccctctggt tgaaaagtca aggatttgaa
    30661 gtggattttg ctccagttga taagaaaggc ttggtcgatg ttgaggcgtt agcaggtttg
    30721 atacggcatg atacaatcct cgtttccatc atggctgtga acaatgaaat cggctctatc
    30781 caacctattg aggctatttc agaattcttg gcagacaagc cgactatttc cttccacgtt
    30841 gatgcggttc aggcgcttgc caaaattccg actgaaaagt atctgacaga acgggtggat
    30901 tgcgcgactt tctctagtca caagttccac ggggttcgag gtgttggctt tgtctatatc
    30961 aaatctggca agaagattac acctcttctt acaggtggtg gccaggagcg agattatcgt
    31021 tcgacaactg aaaatgtggc agggattgca gcgacagcca aggccctccg tttgtctatg
    31081 gaaaagctag atatctttag gagcaagact gggcagatga aggcagtgat tcgccaagct
    31141 cttctgaact atccggatat ttttgtcttt tcagatgagg aaaactttgc acctcatatt
    31201 ctgacttttg gaatcaaagg tgttcgaggt gaagtcatcg ttcacgcctt tgaagactat
    31261 gatattttca tctcaacaac ctcagcttgt tcatctaagg caggaaaacc agccggtacc
    31321 ttgattgcca tgggagtgga caaagataag gccaagtcag ctgtgcgtct tagcctagac
    31381 ttggaaaatg atatgagtca ggtcgagcag tttttgacca agttaaaatt gatttacaat
    31441 caaactagaa aagtaagata ggagcattca tgcagtattc agaaattatg attcgctacg
    31501 gagagttgtc aaccaagggt aaaaaccgta tgcgtttcat caataaactt cgtaataata
    31561 tttcggacgt tttgtctatc tatacccaag ttaaggtaac agcagatcgc gaccgtgccc
    31621 acgcttacct caatggagct gattacacag cagttgcaga atctctcaaa caagtttttg
    31681 gaattcaaaa cttttctcct gtttataagg ttgaaaaatc tgtagaagtt ttgaagtctt
    31741 ctgtccaaga gattatgcgg gacatctaca aggaaggtat gacctttaag atttctagca
    31801 agcgtagcga ccacaacttt gaacttgata gtcgtgaact caaccaaaca cttggagggg
    31861 ctgtattcga agccattcca aatgtgcaag ttcaaatgaa aagtcctgac atcaatcttc
    31921 aggtggagat tcgtgaagaa gcagcctatc tttcttatga aaccattcgt ggggctggtg
    31981 gtttgccagt tggaacttca ggtaaaggga tgctcatgtt gtcaggaggg attgactcac
    32041 ctgtagcagg ttatcttgct cttaagcgtg gggtggatat cgaggcagtt cactttgcta
    32101 gtccaccata tactagtcct ggtgccctca agaaagcgca ggacttgacc cgtaaattga
    32161 ccaagtttgg cggaaatatc cagtttatag aggtgccttt cacagagatt caagaggaaa
    32221 tcaaagccaa agcgccagaa gcttatttga tgactctaac tcgtcgcttt atgatgcgga
    32281 ttactgaccg tattcgtgag gtacgaaatg gtttggttat catcaatggg gaaagtctag
    32341 gtcaagtagc cagccaaacc cttgaaagta tgaaggctat caatgctgtt accaacactc
    32401 ccatcattcg tcctgtggtt accatggaca agttggaaat cattgacatc gcccaggaaa
    32461 tcgatacctt tgacatttca atccaaccgt ttgaagactg ttgtaccatt tttgcaccag
    32521 atcgtccaaa aacaaatcct aaaattaaga atgcggagca gtacgaagcg cgtatggatg
    32581 ttgaaggctt ggttgagcga gcagtggctg gaatcatgat tactgaaatc acacctcaag
    32641 ccgaaaaaga tgaagttgat gacttgattg acaatctgct ctaattcaga aaatccaaaa
    32701 gaatagcgaa aatcagtaaa aaaagttagt tttttctcta aaaacaggta aaaaactaac
    32761 tttttttatt tttatgatat aatgatataa aattttgaat atagagagtt ttctgacaat
    32821 gaatcaatcc tacttttatc taaaaatgaa agaacacaaa ctcaaggttc cttatacagg
    32881 taaggagcgc cgtgtacgta ttcttcttcc taaagattat gagaaagata cagaccgttc
    32941 ctatcctgtt gtatactttc atgacgggca aaatgttttt aatagcaaag agtctttcat
    33001 tggacattca tggaagatta tcccagctat caaacgaaat ccggatatca gtcgcatgat
    33061 tgtcgttgct attgacaatg atggtatggg gcggatgaat gagtatgcgg cttggaagtt
    33121 ccaagaatct cctatcccag ggcagcagtt tggtggtaag ggtgtggagt atgctgagtt
    33181 tgtcatggag gtggtcaagc cttttatcga tgagacctat cgtacaaaag cagactgcca
    33241 gcatacggct atgattggtt cctcactagg aggcaatatt acccagttta tcggtttgga
    33301 ataccaagac caaattggtt gcttgggcgt tttttcatct gcaaactggc tccaccaaga
    33361 agcctttaac cgctatttcg agtgccagaa actatcgcct gaccagcgca tcttcatcta
    33421 tgtaggaaca gaagaagcag atgatacaga caagaccttg atggatggca atatcaaaca
    33481 agcctatatc gactcgtcgc tttgctatta ccatgatttg atagcagggg gagtacatct
    33541 ggataatctt gtgctaaaag ttcagtctgg tgccatccat agtgaaatcc cttggtcaga
    33601 aaatctacca gattgtctga gattttttgc agaaaaatgg taagttaaga aaggaaaaaa
    33661 cgaaatgcat attgaacatc ttagccactg gagtggtcat cttaaccgtg aaatgtacct
    33721 taaccgttat ggacatggtg ggattccagt tgtggtcttt gcttcatcag gtggtagtca
    33781 caacgaatac tatgattttg gcatgattga tgcctgtgct tcctttatcg aggaaggcct
    33841 tgtccagttc tttaccctat ctagtttgga tagtgagagc tggttggcta cttggaaaaa
    33901 tgctcatgac caagcggaaa tgcaccgtgc ctacgaacgt tatgtgattg aggaggccat
    33961 tcttttatca agcacaagac aggttggttt gatggcatga tgacgacagg ttgctctatg
    34021 ggagcctatc atgcactcaa tttcttcctc cagcatccag atgtctttac caaagtgatt
    34081 gctctcagtg gtgtttacga cgcacgtttc tttgtcggtg attactacaa cgatgatgct
    34141 atttaccaaa actcgccagt agattatatt tggaaccaaa acgacggctg gtttattgac
    34201 cgttaccgtc aggcagagat tgtgctgtgt acggggcttg gagcctggga acaagatggt
    34261 ttgccatcct tttacaagct caaagaagcc tttgacaaga aacaaattcc agcctggttt
    34321 gctgaatggg gacatgatgt cgcccatgac tgggaatggt ggcgtaaaca aatgccttat
    34381 ttcctcggta atctctattt ataaaaggag ttacctatga attaccttgt tatttctccc
    34441 tactatccac aaaactttca acagtttacc atcgaactag ctaataaagg catcacagtc
    34501 ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc ccttgcgcaa tagcttgacc
    34561 gagtattttc gtgttgataa tcttgagaac atagatgaag tcaaacgtgc agttgctttt
    34621 ctcttttata aacatggtcc aattggccgc atcgagtctc acaatgaata ctggcttgag
    34681 ctagacgcaa cactcagaga acaattcaat gtttttggtg ccaaaccaga ggatctcaaa
    34741 aagacgaaat ataagtctga aatgaagaaa cttttcaaaa aagcaggtgt tcctgtggta
    34801 cctggagctg ttatcaagac ggaagcagat gttgatcaag cagtgaaaga aatcggtctt
    34861 ccaatgattg ccaaacctga taatggagtg ggagcagccg caacctttaa acttgagaca
    34921 gaagacgata tcaatcactt caagcaagaa tgggaccatt caacccttta tttctttgaa
    34981 aaatttgtca cttccagcga aatctgtacc tttgacgggc tcgtggacaa ggatggaaag
    35041 attgtcttct caacaacctt tgactacgcc tatacaccgc ttgacctcat gatttataag
    35101 atggacaatt cttattatgt gctcaaggat atggatccta aactgcgcaa gtatggggaa
    35161 gcaattgtca aagaatttgg tatgaaagaa cggtttttcc atattgagtt cttccgtgag
    35221 ggggacgatt atattaccat cgagtacaat aaccgccctg caggtggttt taccattgat
    35281 gtttataact ttgctcattc cttggacctt tatcgtggct atgcagctat tgtcgcagga
    35341 gaggagttcc cggcgtcaga ctttgaaact cagtattgtt tggctacttc tcgccgtgca
    35401 aatgctcact atgtttattc agaagaggat ttgcttgcca aatatagcca gcagttcaag
    35461 gttaaaaaag tcatgccagc tgccttcgcg gaacttcaag gagattacct gtatatgctg
    35521 accactccga gtcgacaaga aatggagcag atgattgcag atttcggaca acgtcaagaa
    35581 taagaactat cggattaagg aaattaactc ccttaatcct tttgttttgt ctgataaaaa
    35641 ataagagcat cccaacaagg tagctatcat aaaacttgtt cgataactat ttgaagcagg
    35701 attaggtggt cagaaattaa attttaatat ttcaattgag tcatagtatt gtgtttgcgt
    35761 atccttaaat cagctaaaag gatccatgac gacacctata cgatatagtt ttcaagatac
    35821 caaacaagtc tattaatatt caatgaaaat caaagagcaa actaggaagc tagccgcagg
    35881 tt
//
LOCUS       DAVBCR010000015        35516 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCR010000015 DAVBCR010000000
VERSION     DAVBCR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.015"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..375)
                     /locus_tag="VQ508_000656"
                     /pseudo
     CDS             complement(1..375)
                     /locus_tag="VQ508_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            370..624
                     /locus_tag="VQ508_000657"
     CDS             370..624
                     /locus_tag="VQ508_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2489694.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            968..2302
                     /gene="trmFO"
                     /locus_tag="VQ508_000658"
     CDS             968..2302
                     /gene="trmFO"
                     /locus_tag="VQ508_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2489695.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2386..3123
                     /locus_tag="VQ508_000659"
     CDS             2386..3123
                     /locus_tag="VQ508_000659"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2489696.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3132..3689
                     /gene="frr"
                     /locus_tag="VQ508_000660"
     CDS             3132..3689
                     /gene="frr"
                     /locus_tag="VQ508_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2489697.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3749..4603
                     /gene="cvfB"
                     /locus_tag="VQ508_000661"
     CDS             3749..4603
                     /gene="cvfB"
                     /locus_tag="VQ508_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2489698.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4612..4827
                     /locus_tag="VQ508_000662"
     CDS             4612..4827
                     /locus_tag="VQ508_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2489699.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4913..5881
                     /locus_tag="VQ508_000663"
     CDS             4913..5881
                     /locus_tag="VQ508_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2489700.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            6061..6561
                     /locus_tag="VQ508_000664"
     CDS             6061..6561
                     /locus_tag="VQ508_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489701.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6564..6824
                     /locus_tag="VQ508_000665"
                     /pseudo
     CDS             6564..6824
                     /locus_tag="VQ508_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7191..8302)
                     /gene="ald"
                     /locus_tag="VQ508_000666"
                     /pseudo
     CDS             complement(7191..8302)
                     /gene="ald"
                     /locus_tag="VQ508_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8479..9048
                     /locus_tag="VQ508_000667"
     CDS             8479..9048
                     /locus_tag="VQ508_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489702.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9116..9766
                     /locus_tag="VQ508_000668"
     CDS             9116..9766
                     /locus_tag="VQ508_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2489703.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9750..11990
                     /locus_tag="VQ508_000669"
     CDS             9750..11990
                     /locus_tag="VQ508_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2489704.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12170..12358
                     /locus_tag="VQ508_000670"
     CDS             12170..12358
                     /locus_tag="VQ508_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489705.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12390..12977
                     /locus_tag="VQ508_000671"
     CDS             12390..12977
                     /locus_tag="VQ508_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489706.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12981..14156
                     /locus_tag="VQ508_000672"
     CDS             12981..14156
                     /locus_tag="VQ508_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489707.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14330..14461
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14477..15007
                     /gene="infC"
                     /locus_tag="VQ508_000673"
     CDS             14477..15007
                     /gene="infC"
                     /locus_tag="VQ508_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2489708.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15040..15240
                     /gene="rpmI"
                     /locus_tag="VQ508_000674"
     CDS             15040..15240
                     /gene="rpmI"
                     /locus_tag="VQ508_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2489709.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15292..15651
                     /gene="rplT"
                     /locus_tag="VQ508_000675"
     CDS             15292..15651
                     /gene="rplT"
                     /locus_tag="VQ508_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2489710.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15709..16089
                     /locus_tag="VQ508_000676"
     CDS             15709..16089
                     /locus_tag="VQ508_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2489711.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16339..17139
                     /locus_tag="VQ508_000677"
     CDS             16339..17139
                     /locus_tag="VQ508_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2489712.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            17102..18088
                     /locus_tag="VQ508_000678"
     CDS             17102..18088
                     /locus_tag="VQ508_000678"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2489713.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18130..20031
                     /locus_tag="VQ508_000679"
     CDS             18130..20031
                     /locus_tag="VQ508_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2489714.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(20092..21747)
                     /gene="pavA"
                     /locus_tag="VQ508_000680"
     CDS             complement(20092..21747)
                     /gene="pavA"
                     /locus_tag="VQ508_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2489715.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21852..22349
                     /gene="ybeY"
                     /locus_tag="VQ508_000681"
     CDS             21852..22349
                     /gene="ybeY"
                     /locus_tag="VQ508_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2489716.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22330..22725
                     /locus_tag="VQ508_000682"
     CDS             22330..22725
                     /locus_tag="VQ508_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2489717.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22742..23641
                     /gene="era"
                     /locus_tag="VQ508_000683"
     CDS             22742..23641
                     /gene="era"
                     /locus_tag="VQ508_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2489718.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23690..24514
                     /gene="mutM"
                     /locus_tag="VQ508_000684"
     CDS             23690..24514
                     /gene="mutM"
                     /locus_tag="VQ508_000684"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2489719.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24514..25119
                     /locus_tag="VQ508_000685"
     CDS             24514..25119
                     /locus_tag="VQ508_000685"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2489720.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25106..26305
                     /locus_tag="VQ508_000686"
     CDS             25106..26305
                     /locus_tag="VQ508_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2489721.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26305..26454
                     /gene="rpmG"
                     /locus_tag="VQ508_000687"
     CDS             26305..26454
                     /gene="rpmG"
                     /locus_tag="VQ508_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2489722.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26494..26727
                     /gene="secG"
                     /locus_tag="VQ508_000688"
     CDS             26494..26727
                     /gene="secG"
                     /locus_tag="VQ508_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2489723.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26829..29183
                     /gene="rnr"
                     /locus_tag="VQ508_000689"
     CDS             26829..29183
                     /gene="rnr"
                     /locus_tag="VQ508_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2489724.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29146..29613
                     /gene="smpB"
                     /locus_tag="VQ508_000690"
     CDS             29146..29613
                     /gene="smpB"
                     /locus_tag="VQ508_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2489725.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NIKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29629..30489
                     /gene="tehB"
                     /locus_tag="VQ508_000691"
     CDS             29629..30489
                     /gene="tehB"
                     /locus_tag="VQ508_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2489726.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30562..31515
                     /gene="coiA"
                     /locus_tag="VQ508_000692"
     CDS             30562..31515
                     /gene="coiA"
                     /locus_tag="VQ508_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2489727.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31534..33336
                     /gene="pepF"
                     /locus_tag="VQ508_000693"
     CDS             31534..33336
                     /gene="pepF"
                     /locus_tag="VQ508_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2489728.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33338..34051
                     /locus_tag="VQ508_000694"
     CDS             33338..34051
                     /locus_tag="VQ508_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2489729.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34118..35059
                     /gene="prsA"
                     /locus_tag="VQ508_000695"
     CDS             34118..35059
                     /gene="prsA"
                     /locus_tag="VQ508_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2489730.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35176..>35516)
                     /locus_tag="VQ508_000696"
     CDS             complement(35176..>35516)
                     /locus_tag="VQ508_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2489731.1"
                     /translation="LEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRK
                     GNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLS
                     PVQYRTKSFR"
BASE COUNT        10674 a         6606 c         8007 g        10229 t
ORIGIN      
        1 tttttagggc gattgatttg tacttccgtc gcatcaatca ttaccgtgtc ctcagaactg
       61 agaggagttc ttgaaatcgt aacaccacct ttgaacaaga gttacttcaa cccattggct
      121 ccgacggagt aagttgcttt cgtggatacc aaaatcagcc gcaatttctt cataagtgcg
      181 atattctcgc acatattgaa gagtggccat aagaaggtct tctaggctta atttaggttt
      241 tcgtccccct tttgcgtgtt taagttgata agctgttttt aatacagcta atatctcttc
      301 aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
      361 tgcttcataa ttcatagaac tactatacca tattttgttt cgcaggaagt ctattggaaa
      421 gtaagaaata ttggagctga ggcgattaga agaaattgtg agcgtggtgc tattttttca
      481 ggtaaaataa aacatcacga agattctcag tttaaaggag accactatgt tgaatgttat
      541 gctgttttag ataatacggt tatagcaaga gatagaataa cagtccctat cgatccgtta
      601 tgtggaaaag attttataga gtagcatata attgattctt aactggaata ctctctatct
      661 ctttacatca agaaaatgac taaacaggga agtttgcctt cttccctttt tttgttatac
      721 tagtagaaga aaaaaataga aagatttgtg ggagtgaaaa agtccggggg actttttcag
      781 cttgagccaa gaaattcgag agcgaggaaa gtccggggga ctttttcagc ctgagccaag
      841 aaattcgaga gcgaagtaag tccaatggac tttttcagcc tgagccaaga aactcgaaag
      901 ctcgtaagtc tgattggctt tttcaatgtg aatcttaact tcacactccc aaagaggtat
      961 tagtgtcgtg tctcaatctt atatcaatgt tatcggtgct ggtttggcag gttctgaagc
     1021 agcttaccaa atcgcagagc gtggtattcc agttaaacta tatgaaatgc gtggtgtcaa
     1081 gtcgactccc cagcataaaa cagacaattt tgctgagttg gtttgttcca attctttgcg
     1141 tggggatgct ttgacaaatg cagttggtct tctcaaggaa gaaatgcgtc gcttgggttc
     1201 tgttatcttg gaatctgctg aggctacacg tgttcctgca ggtggtgccc ttgcagtgga
     1261 ccgtgatggt ttctctcaaa tggtgaccga aaaagttgcc aaccacccct tgattgaagt
     1321 ggttcgtgat gaaattacag aattgccgac agatgttatt acggttatcg ctactggtcc
     1381 tttgacaagt gatgccttgg ctgaaaagat tcatgccctt aatgacggtg atggttttta
     1441 tttctacgat gcggcagcgc ctattatcga tgtcaacact atcgatatga gcaaggtcta
     1501 cctcaaatca cgttatgata agggagaagc ggcctacctc aatgccccta tgaccaagca
     1561 agaatttatg gatttccatg aagctttggt caatgcagaa gaagcaccgc ttaattcttt
     1621 tgaaaaagaa aagtactttg aaggctgtat gcctatcgaa gtcatggcca aacgtggcat
     1681 taaaactatg ctttatggcc ctatgaagcc agtcggtctt gagtacccag acgactatac
     1741 aggacctcgt gatggagaat ttaaaacacc ttatgcggtt gtgcaacttc gtcaggacaa
     1801 tgcagctggt agcctctaca atattgttgg tttccagacc cacctcaaat ggggagaaca
     1861 aaagcgtatc ttccaaatga ttccgggtct tgaaaatgca gagtttgtcc gttatggtgt
     1921 catgcatcgc aattcttaca tggattcacc aaatcttctt gagcagactt accgttctaa
     1981 gaaacaacca aatctcttct ttgctggtca gatgacgggg gtggaaggct atgttgagtc
     2041 ggcggcttca ggcttagttg cgggaattaa cgcagctcgt ctcttcaagg aagaaagcga
     2101 ggctattttc cccgagacga cagcgattgg aagcttagct cattacatta cccatgccga
     2161 cagcaaacat ttccaaccaa tgaatgtcaa ttttgggatc atcaaggagt tggaaggcga
     2221 gcgtatccgt gataagaagg ctcgttatga aaaaattgca gagcgtgccc ttgccgactt
     2281 agaggaattt ttgactgtct aatttttttg aaagaattgc tcatgatact ataaaaatct
     2341 tagaaattgt gataaaatag gtaggatgaa agaaggagag tgaaaatggc gaatcccaag
     2401 tataaacgta ttttaatcaa gttatcaggt gaagcccttg ccggtgaacg tggcgtaggg
     2461 attgatatcc aaacagttca aacaatcgca aaagagattc aagaagttca tagcttaggt
     2521 atcgaaattg cccttgttat tggtggagga aatctctggc gtggagaacc tgcagcagaa
     2581 gcaggtatgg accgtgttca ggcagattac actggaatgc ttgggactgt tatgaatgct
     2641 cttgtgatgg cagattcatt gcaacaagtt ggggttgata cgcgtgtaca aacagctatt
     2701 gctatgcaac aagtggcaga gccttatgtc cgtggacgtg cccttcgtca ccttgaaaaa
     2761 ggccgtatcg ttatctttgg tgctggaatt ggttcaccat acttctcgac agatacaaca
     2821 gcggcccttc gtgcagctga aatcgaagcg gatgccatcc tcatggctaa aaatggtgtc
     2881 gatggtgttt acaatgccga tcctaagaaa gataagacag ctgttaagtt tgaagaattg
     2941 acccaccgtg acgttatcaa taaaggtctt cgtatcatgg actcaacagc ttcaaccctc
     3001 tcaatggaca acgacattga cttggttgtc ttcaacatga accaatcagg caacatcaaa
     3061 cgtgtcgtat ttggtgaaaa tatcggaaca acagtttcaa ataatatcga agaaaaggaa
     3121 taagaaagaa tatggctaac gcaattattg aaaaagctaa agagagaatg acccagtctc
     3181 accaatcact tgctcgtgaa tttggtggta tccgtgctgg tcgtgccaat gcaagcttgc
     3241 ttgaccgtat acatgtagaa tactatggag ttgaaactcc tcttaaccaa atcgcttcaa
     3301 ttacgattcc agaagcgcgt gttttgttgg taacaccatt tgacaagtct tcattgaaag
     3361 acatcgaacg tgccttgaac gcttctgatc ttggtatcac accagctaat gacggttctg
     3421 tgattcgctt ggttgtccca gctcttacag aagagactcg tcgtgacctt gctaaagaag
     3481 tgaagaaggt cggcgaaaat gctaaagtgg ctgtccgcaa tatccgtcgc gatgctatgg
     3541 acgaagctaa gaaacaagaa aaagcaaaag aaatcactga agacgaattg aagactcttg
     3601 aaaaagacat tcaaaaagta acagacgatg ctgttaaaca catcgacgat atgactgcca
     3661 acaaagagaa agaacttttg gaagtctaaa aataaacaga aaaactcagt tggcattgct
     3721 ggctgagttt tattcgaaag aaggaaatat gaatacaaat cttgcaagtt ttatcgttgg
     3781 actgatcatc gatgaaaacg accgttttta ctttgtgcaa aaggatggtc aaacctatgc
     3841 tcttgctaag gaagaaggcc aacatacagt aggggatacg gtcaaaggtt ttgcatacac
     3901 ggatatgaag caaaaactcc gcctgacaag cttagaagtg actgccactc aggaccaatt
     3961 tggttgggga cgtgtcacag aggttcgtaa ggacttgggt gtctttgtgg atacaggcct
     4021 tcctgacaag gaaatcgttg tgtcactcga tattctccct gagctcaagg aactctggcc
     4081 taagaagggc gaccaactct acatccgtct tgaagtggat aagaaagacc gtatctgggg
     4141 tctcttggct tatcaagaag acttccaacg tcttgctcgt cctgcctaca acaacatgca
     4201 gaaccaaaac tggccagcca ttgtttaccg tctcaagcta tcaggaactt ttgtttacct
     4261 accagaaaat aatatgcttg gttttattca tcctagcgag cgttacgcag agccacgttt
     4321 ggggcaagta ttagatgcgc gcgttattgg tttccgtgaa gtggaccgca ctttgaacct
     4381 ctccctcaaa ccacgctcct ttgaaatgtt ggaaaacgat gctcagatga ttttgactta
     4441 tttggaaagc aatggcggtt tcatgacctt aaatgacaag tcatctccag acgacatcaa
     4501 ggcaaccttt ggcatttcta aaggtcagtt caagaaagct ttaggtggtc ttatgaaggc
     4561 tggtaaaatc aagcaggacc agtttggtac agagttgatt tagggaggct tatgagaaaa
     4621 tcattttaca cttggctcat gaccgagcgc aatcctaaaa gtaacaatcc caaagcaatt
     4681 ttggcagacc tcgcttttga agaggcagct tttccaaaac atacagatga ttttgatgag
     4741 gtcagtcgct ttttggagga gcatgccagt ttctctttta acctaggaga ttttgacagc
     4801 atttggcagg aatatctaga acactagcat ttattcattg ggtttgggct agtaatttct
     4861 ccatccctct gctataataa aaagaaataa aaggattaga gaggttcttt atttgaagga
     4921 acattcaata gacattcaac tgagtcatcc agatgacctg tttcatcttt ttggttccaa
     4981 tgaacgccat cttcgtttga tggaagaaga gcttgatgtt gtgattcatg ctcgtacgga
     5041 gattgtccag gttttgggag aagagtctgc ttgcgaggaa gcccgtcagg ttattcaagc
     5101 tctcatggcc ttggtaaatc gtgggatgac cgttggtacg ccagatgtag tgacggctat
     5161 tagtatggtc aaaaatgatg aaattgacaa gtttgtcgcc ctttacgaag aagaaatcat
     5221 caaggataat actgggaagc ctatccgcgt caaaacctta ggtcaaaaac tttatgtgga
     5281 cagtgtcaaa cagcatgatg tgacctttgg aattgggcca gcaggtacag ggaagacctt
     5341 ccttgcagtg accttggcag tgactgccct taaacgtggg caagtcaagc gaattatcct
     5401 aactcgtcca gcggtggaag cgggagagag tcttggattt cttccgggtg atcttaagga
     5461 gaaggtggat ccttaccttc gtcctgttta cgatgccttg tatcaaattc ttgggaaaga
     5521 ccaaacgact cgtctcatgg agcgtgaaat tatcgaaatt gcgccccttg cctatatgcg
     5581 tggccggacc ttggatgatg cctttgtcat tctcgatgag gcgcaaaaca cgaccatcat
     5641 gcagatgaag atgttcttga cgcgtttagg ctttcattct aagatgattg tcaatggaga
     5701 tatcagtcag attgacctgc cacgtaatgt caagtccggt ttgattgatg ctcaagagaa
     5761 actcaagaac atccatcaga ttgactttgt tcatttttca gccaaggatg tggttcgcca
     5821 tccagttgtc gctcagatta tccgagccta tgaatattct actgaagttg cacacgactg
     5881 attttgagga agttcgcctg caaaagaata tacttttgag agaggaaaat ccagttgtat
     5941 aggctaaagg tttgagacaa cgattaggca cgatggaaag aacttttatg tggctgatga
     6001 cgatcagtgc atcttcctgt gtcataatca cagggcacaa gaaagtagga atttgaaaag
     6061 atgattgacc aactatctaa gtattacagt tgtaggatac taactgaaaa ggatattcca
     6121 agtattttat ctttatatga aagtaatcct ctgtattttc agcattgtcc accagagcca
     6181 aattttgcaa ctgtaaaaga ggacatgctt tgtctacctg aaggtaaagc taaggctgat
     6241 aagttttttg ttggattttg gaatggctct gaccttgtgg ctgttatgga ttttgtctat
     6301 gcatatcctg atgaggagac tgtttttatt ggtttgttta tggttgatca agcctatcag
     6361 aggaaaggga ttggtagtca tattgtgaca gaagcactag cttattttgc taagaacttt
     6421 cgaaaggcac gtttggctta tgttaaggga aatccgcaat ctcagcattt ttgggaaaag
     6481 cagggcttta aatcaattgg atgcgaggtt aagcaagaac tctatacggt tgttatcgct
     6541 gaacagagcc tagaagatta gaaatggcat caagtaagaa ctatttggaa tttgttttgg
     6601 aacaattatc aggattagat gatgtgactt accgttccat gattggggag tatattcttt
     6661 acttccgcgg caagattatt ggcggcattt atgacgatcg ctttttagtt aaacccgtgc
     6721 aagcagtctt agataagatt gaccaatctt cttttgagtt tccatacaaa ggtgccaaag
     6781 aaatgatttg agtggaagaa cttgataata agatgtttct ataagacctg attttagcta
     6841 tgtataacca actgccaacg cccaaaccta aaaagaaaaa gcaagggtga acgaagtaaa
     6901 aaagaagtct gctaaggccc tgtctttgca cgggtaaaat tttatatata aaaagaagct
     6961 gggactaaag agctcagctt cctttggttt atataattgt cattacaaga cgaagtggtt
     7021 gggcgaaact ctgttgactt tattcaattt agagtttctt atgcacaatt gagtctggaa
     7081 cgaaagtctc cagttgcaaa gtatacagta caataaacca acgatgtaat agctgatgac
     7141 acaaagcaca gtgggtagga cttgcgaagt cacccttttc ttttcaaaat ttatactaaa
     7201 tcattgatat cagtgtagtc acgattaagt ccttgagcaa ctggtaggct agtcaagtaa
     7261 ccttgataag tggtcacacc ttgacgcaag ccttcatctt cagagattgc ttgtgcgaat
     7321 cctttgccag ccaaagcttc gatataagga agagtgacat tggttagggc gatggttgaa
     7381 gtgcgggcaa ccgcaccagg gatattggca acggcatagt ggagaacacc gtgtttttca
     7441 tagacgggtt catcgtgcgt tgtcacacgg tcagctgttt cgataacgcc accttggtca
     7501 acagcaacgt caacgataca gagcctggac gcatttgttt gaccatctca tctgtcacca
     7561 attccggtgc ttttgcacca gggatgagaa tggctccaat caccacatca gcatctctca
     7621 cacttgcttc aatgttgaat gaattagata taatagtttg aatttgactt ccaaagactt
     7681 cttctagaac tgagagacgc ttggcactaa tatctaaaat agtcacttga gcaccaagac
     7741 caagggcgat gcgggcagca tgtgtaccga cgacaccatc accgatgata gttacttttc
     7801 cttttggaac acctggtaca ccaccaagta gaacaccaga gccaccagct tgcttagtaa
     7861 ggaagtgagc tccgatttga acagccatac gacctgcaac ctcactcata ggaacgagga
     7921 gcggtagttg tccttgattg tcacgaacag tttcataggc aattcctgtt gtttttgctg
     7981 ctaacatagc atctgctaat tctggagcag cggccatgtg caagtaggtg aagagaagaa
     8041 gatcgtcgcg caagtaaccg tattcagaac ttaaaggttc ttttactttc acaaccaact
     8101 ctgctgccca agcttcacca gcagtagcga caatctcagc tccttgcttt tgatagtcag
     8161 catcagtaaa gccagaaccg agaccagcat ttgtttcgat aaggacacga tgaccacgac
     8221 taactaagct atgaacacct gcaggtgtga gggcgacacg gttttcgtta tttttaattt
     8281 cttttgggat tccgattaac attgagataa cctacctttc aattgacggt cttgttttgg
     8341 ttgtcacatt ccagttcata aatcaaaaat gtgacggttt cattgtatat gaaaccgctt
     8401 caaaaatcaa gaaaaacttg tcatccaaat ttttttatgc tagactagtg aaaatcaagc
     8461 tctaatggag ggaaaagtat ggaatcaata tttgtgaaat ttgcccagta tccgtctata
     8521 gaaacggagc gtttattgct tagacctgta actttggatg atgcggaagc aatgtttgac
     8581 tatgcctcgg acaagggtaa tacacgttac acttttccaa ccaatcaaag cttggaagaa
     8641 accaagaata acattgctca gttctacttg gctaatccct tgggacgttg ggggattgaa
     8701 ttaaaagata acggcaagtt tatcggaact attgacttac acaagattga tcctgttctt
     8761 aagaaggcag ctattggcta cattatcaat aaaaagtatt ggaatcaagg attaacgaca
     8821 gaagccaatc gtgctgtgat tgagctagct tttgagaaga tagggatgaa taagttgact
     8881 gcccttcacg ataaggctaa tcccgcgtca ggaaaggtca tggagaaatc aggcatgcgt
     8941 ttttcccatg cagaaccata tgcttgtatg gaccagcatg aaaaaggccg aatcgtgaca
     9001 agagttcatt atgtcttgac caaggaagac tattttgcaa ataaataagc agttgaaaag
     9061 aaatttttcg actgtttttt cttcctctta cgaataatct aagagaggag aaaatatgga
     9121 agcaattatc gagaaaatca aagagtataa aatcatcgtc atctgtactg gtctgggctt
     9181 gcttgtagga ggatttttcc tgctaaagcc agctccacaa acatctgtca aagagacgaa
     9241 tttgcaggct gaagttgcag ctgtttccaa ggactcatcg accggaaagg aagtgaacaa
     9301 ggaagagaag gaagaaccag ttgaacaaga tctaatcaca gtagatgtca aaggtgctgt
     9361 caaatcgcca gggatttatg acttgcctgt aggtagtcga gtcaatgatg ctgttcagaa
     9421 ggctggtggc ttgacagagc aagcagacag caagtcgctc aatctagctc agaaagttag
     9481 tgatgaggct ctggtttacg ttcctactaa gggagaagaa gcagttagtc aacagactgg
     9541 ttcggggaca gcttcttcaa caagcaagga aaagaaggtc aatctcaaca aggccagtct
     9601 ggaagaactc aagcaggtca agggactggg aggaaaacga gctcaggaca ttattgacca
     9661 tcgtgaggca aatggcaagt tcaagtcagt agacgagctc aagaaggtct ctggcattgg
     9721 tgccaagacc atagaaaaac ttaaagacta tgttacagtg gattaagaat ttctctattc
     9781 ccctaattta cctgagtttt ctattactat ggctttacta cgccattttc tcagtatcct
     9841 attttgcttt gttgggtttt gtttttctgc tagtctgcct ctttatccaa tttccttgga
     9901 aatcagcagg taaagttcta gtgatttgtg gagtctttgg cttctggttt ctgtttcaaa
     9961 cttggcaaca gagtcaagtg agtcaaaatt tggtggattc tgttgaaagg gtacggattt
    10021 taccagacac tattaaggtt aacggtgaca gtctgtcctt tcgtggtaag tctgatggtc
    10081 gtgctttcca agtctattat aaactccagt ccgaggagga gaaagaagcc tttcaagctt
    10141 taactgacct tcatgagata ggactagaag ggaagctttc ggagccagaa gggcagagaa
    10201 attttggtgg ctttgactac caaggctatc tgaagactca gggaatttac cagacactca
    10261 atattaaaag aatccagtca ctccaaaagg ttggcagttg ggatataggt gaaaacctgt
    10321 ccagtttacg tcgaaaggct gtggtttgga ttaagatgca cttcccagac cctatgcgca
    10381 attacatgac aggactcttg ctaggacatc tggacaccga ttttgaggag atgaatgagc
    10441 tttattccag tttaggaatt attcaccttt ttgccttgtc aggtatgcag gtagggtttt
    10501 tcatggacgg atttaagaaa ctacttttac gattgggctt gacacaagaa aagttgaagt
    10561 ggctgactta tcccttttcc cttatctatg cgggactaac tggattttca gcatcggtta
    10621 ttcgcagtct cttgcaaaag ctactggctc aacatggggt taagggcttg gataattttg
    10681 ccttgacggt gcttgtcctc tttattgtca tgccaaactt tttcttgaca gcaggaggag
    10741 tcttgtcctg tgcttatgct tttatcctga ccatgaccag caaagaaggg gaggggctca
    10801 aggctgttac tagtgaaagt ctagtcatct ccttgggcat attgcccatt ctatccttct
    10861 attttgcgga atttcaacct tggtctatcc ttttgacctt tgtcttttcc tttctttttg
    10921 acttggtctt cttaccgctc ttgtctatct tatttgtcct ttcctttctc tatccagtca
    10981 ttcagctgaa ctttatcttt gaatggttag agggcattat tcgcttggtc tcgcaggtgg
    11041 caagtagacc gcttgtcttt ggacaaccca atgaatggct tttaatccta ttgttaattt
    11101 ctttggcttt ggtctatgat ttgaggaaaa acattaaaaa gctaacggta ttgtgcttat
    11161 tgattacagg gctctttctc ctgaccaagc atccactgga aaatgaaatc accatgctgg
    11221 atgtggggca aggagaaagt attttcctac gggatgtaac tgggaaaacc attctcatag
    11281 atgtaggtgg taaggcagaa tcttataaga aaatcaaaaa atggcaagaa aagatgacga
    11341 ccagcaatgc ccagcgaagc ttgataccct atctcaaaag tcgaggagta gctaagattg
    11401 accagctaat tttgactaac acggacaagg agcatgttgg agatttgtca gagatgacca
    11461 aggctttcca tgtaggggag attctagtat caaaagacag tctgaaacag aaggaatttg
    11521 tggcagaact acaggcgact caaacaaagg tgcgtagtat gatagtaggg gagaacttac
    11581 ccatttttgg aagtcagtta gaagttctat ctccaaggaa aatgggagat ggaggacacg
    11641 atgataccct agttctgtat gggaaattct tggataagca atttctcttc acgggaaatt
    11701 tggaggagaa aggagagaag gacttgctga agcactatcc agacttgaaa gtaaatgttt
    11761 tgaaagctag ccaacatggc aataaaaaat catcaagtcc agcctttcta gaaaaactca
    11821 aaccagagct tactcttatc tcagttggaa agagcaatcg aatgaaactc ccccatcagg
    11881 aaacattgac acgactggaa ggtatcaata gcaaagttta tcgaactgac cagcaaggag
    11941 ctatacgttt taaggggttg gatagttgga aaatcgaaag tgttcgatag gaaggataaa
    12001 tgttgtagat tagtgaaata aactaaaaat ttgttgcata ataatgataa aaacggcata
    12061 atgaaaacgt attcaatatt gaggatataa aatcattaaa aatcagcaaa agttgtttta
    12121 gttagttagt ttataatcta ttggtcttct tcagtccagt gtatctgctg tgacagtcac
    12181 taaaagttac aagtatgatt ggaatacggt tttggaatat agtaccaact atcacgacca
    12241 tcagtatgct tggattccgg aatggtctcg ttattacagc tattctgagt ataaagttgg
    12301 cggaggctgg aactacgctc gttatgaggt cataaactac tatactggag gttattaatc
    12361 cttaaagagt gagaaaagga gggctggata tgttaaatct tactcatgtt accttaaaaa
    12421 cgcgacaagt catcttgcaa gatgcggatt ttacctttaa aaagggtagg atttatggcc
    12481 ttcttgctat caatggctcg ggaaagacga cactattccg agctatgagc aagttgcttc
    12541 cccttagtag tggacacatc gcagttcctc cttctttgtt ttattatgag agcgttgaat
    12601 ggctggatgg aaacttaagt gggatggact accttcgtct cataaaaaac atctggaagt
    12661 cagacctaaa cttgagagat gaaatcgcct actgggaaat ggctgactat atcagtcttc
    12721 ccatccgcaa gtattcctta gggatgaagc aacgcttggt gattgctatg tattttctca
    12781 gtcaggccaa atgctggctc atggatgaga ttacaaatgg cttagacgag tattatcgac
    12841 agaagttttt tgataggcta gcacaaatcg atagacaaga acagctggtt cttttaagtt
    12901 cccactataa ggaagagttg gttgatatct gcgatagagt agtaaccatt catcaggggc
    12961 agatagaaga ggtttagttt atgaaagatg ttagtctatt tttattgaaa aaagttttca
    13021 aaagtcgttt aaactggatt atcttagctt tatttgtatc tgtactcggt gttacctttt
    13081 atttaaatag tcagactgca aactcacaca gcttggagag caggttggaa agtcgcattg
    13141 cagccaacga gagggctatc aatgaaaatg aagagaaact ctcccaaatg tctgatacca
    13201 gctcggagga ataccagttt gctaaaaata atttagacgt gcaaaaaaat cttttgacgc
    13261 gaaagacaga aattctaact ttactaaaag aagggcgctg gaaagaagcc tactatctgc
    13321 agtggaaaga tgaagagaag aattatgaat ttgtatcaaa tgacccgact gctagctctg
    13381 gcttaaaaat gggggttgac cgcgaacgga agatttacca agccctgtat cccttgaaca
    13441 taaaagcaca tactttggag tttccgaccc acgggattga tcagattgtc tggattttag
    13501 aggttatcat cccaagcttg tttgtggttg cgattatttt tatgctaaca caactatttg
    13561 cagaaagata tcaaaatcat ctggacacag ctcacttata tcctgtttca aaagtgacat
    13621 ttgcaatatc ctctcttgga gttggagtgg gatatgtaac tgtgctgttt atcggaatct
    13681 gtggcttttc ttttctagtg ggaagtctga taagtggttt tggacagtta gattatccct
    13741 acccaattta tagcttagtg aatcaagaag taactattgg gaaaatacaa gatgtattat
    13801 ttcctggctt gctcttagct ttcttagcct ttatcgtcat tgtggaagtt gtgtacttga
    13861 ttgcttactt tttcaagcaa aaaatgcctg tcctctttct ttcactcatt gggattgttg
    13921 gcttattgtt tggcatccaa acaattcagc ctcttcaaag gattgcacat ctgattccct
    13981 ttacttactt gcgttcagtg gagattttat ctggaagatt acctaagcag attgataatg
    14041 tcgatctaaa ttggagcatg ggaatggtct tacttccttg cctgattatc tttttgctat
    14101 tgggaattct atttattgaa agatggggaa gttcacagaa aaaaagaatt ttttaataga
    14161 ttctagcttt cctataggta gggaaaataa gtaaaaacta acatagagag ggaatcaact
    14221 tgattctctc tttttgattc gaaaaccaaa ccaaaataca aacacaaact tttcaaaaaa
    14281 taacttttta tcttgacaag agctagaaaa cttggtatca tataaaagtt gagaaaagca
    14341 gaagtgagag cttctcgcct tgtgacatta agttgcctgg ccctacggat gaaaagtttc
    14401 gaagaaacgc tatcataacg tgcgggcttg tatatttacg agtccgctat tgtttttctc
    14461 taataaaaca aaagaggtga aaaccatagc aaagcaagac ttattcatca atgatgagat
    14521 tcgtgtacgt gaagttcgct tgattggtct tgaaggagaa cagctaggca tcaagccact
    14581 cagtgaagcg caagctttgg ctgataacgc taatgttgac ctagtattga ttcaacccca
    14641 agccaaaccg cctgttgcaa aaattatgga ctacggtaag ttcaaatttg agtaccagaa
    14701 gaagcaaaaa gaacaacgta aaaaacaaag cgttgttact gtgaaagaag ttcgtctaag
    14761 tccaactatt gacaagggtg actttgatac aaaacttcgt aatgcacgta agttccttga
    14821 aaaaggaaat aaagttaagg tatctattcg ctttaagggt cgtatgatta cccataaaga
    14881 gattggtgca aaagttttag ccgagtttgc tgaagcaact caagatattg ccatcatcga
    14941 acaacgtgct aaaatggatg gacgtcaaat gttcatgcaa ttggcgccag cgactgacaa
    15001 aaaataattg tcagaaagtt aaataaagga gaaaaaatta tgccaaaaca aaaaacacac
    15061 cgcgcatcag ctaaacgttt caaacgtaca ggttctggtg gacttaaacg tttccgtgct
    15121 tacacttctc accgtttcca cggaaaaact aagaaacaac gtcgtcatct tcgtaaagca
    15181 tctatggtgc attcaggaga ttacaaacgt atcaaagcaa tgcttactcg cttgaaataa
    15241 tagcttgact gtaagtaaac aattctagga aatatttgga ggaaataaaa catggcacgt
    15301 gttaaaggtg gcgttgtatc acgcaaacgt cgtaaacgta ttcttaaatt agcaaaaggt
    15361 tactatggag ctaaacacat cttgttccgt actgcaaaag aacaagtaat gaactcttac
    15421 tactatgcat accgtgaccg tcgtcagaaa aaacgtgact tccgcaaatt gtggatcact
    15481 cgtatcaatg cggcagctcg tatgaacgga ctttcatact cacaattgat gcatggtttg
    15541 aaattggctg agatcgaagt taaccgtaaa atgcttgctg acttggctgt taacgatgca
    15601 gtagctttca cagctcttgc agatgcagct aaagcaaaac ttggtaaata acaactgatg
    15661 agactggaac aggattgtcc agtcttttta aaaataaagg agaaaaatat ggcttcaaaa
    15721 atgctacaca cttgcttacg agtagaaaat cttgaaaaat caatcgcatt ttatcaagat
    15781 gcttttggtt ttaaagaatt gcgtcgcaga gattttccag accatgcctt cacgattgtc
    15841 tatctaggtc ttgagggtga tgactatgag ttggagttga cttataacta cgatcacggt
    15901 ccgtatgtgg ttggtgatgg gtttgcccat atcgccctca gtacacctga tcttgaggca
    15961 cttcatcaag aacacagtgc aaaaggctat gaagtgactg agccaaatgg tctaccagga
    16021 actacaccaa actattactt tgtcaaagac cctgatggat ataaggtcga agtcattcgt
    16081 gaaaaataat ttcgtgcaag ttaagtagaa tattcgtttt ttacttgact ttttttagct
    16141 gaaagagtat actaataaaa attcaacctt taaggggagt ccagagagac tcacaaggtg
    16201 tcagataaaa gaatagtgca attttctaga ggagactttt tgagtgtgct ctcttgtgtt
    16261 gtacgatttt aactgaggcc ttgcactagc aaggtctttt ctttatctgg tccccttaaa
    16321 atttaaggag gaaaagttat gaatcccaca tgtaagaagc gtttgggtgt cattcggttg
    16381 gaaaccatga aggtggttgc acaagaggaa atcgcgccag caatctttga attagtccta
    16441 gaaggagaaa tggttgaagc catgcgagca ggccaatttc ttcatctgcg tgtaccagac
    16501 gatgcccatc ttttacgtcg tcctatttca atttcgtcta ttgacaaggc aaacaagcag
    16561 tgtcacctca tttatcggat tgagggagct gggactgcaa ttttttcaac cttaagtcag
    16621 ggagacactc ttgatgtgat ggggcctcag ggaaatggtt ttgacttgtc tgaccttgat
    16681 gagcagaatc aggttctcct tgttggtggt gggattggtg ttccaccctt gcttgaggtg
    16741 gccaaggaat tgcatgaacg tggagtgaaa gtagtgacag tcctcggttt tgctaataag
    16801 gatgctgtta ttttgaaaac ggaattggct cagtatggtc aggtctttgt aacgacagat
    16861 gatggttctt atggcatcaa gggaaatgtt tccgttgtta tcaatgattt agatagtcag
    16921 tttgatgctg tttactcgtg tggggctcca ggaatgatga agtatatcaa tcaaaccttt
    16981 gatgatcacc caagagccta tttatctctg gaatctcgta tggcttgtgg gatgggagct
    17041 tgctatgcct gtgttctaaa agtaccagaa agcgagacgg tcaaccaacg cgtctgtgaa
    17101 gatggtcctg ttttccgcac aggaacagtt gtattataag gagaaaatta tgactacaaa
    17161 tcgattacaa gtgtctctac ctggtttgga tttgaaaaat ccgattattc cagcatcagg
    17221 ttgttttggc tttggacaag agtatgccaa gtactatgat ttaaaccttt taggttctat
    17281 tatgatcaag gcgacaaccc ttgaaccacg ttttgggaac ccaactccaa gagtagcaga
    17341 gacgcctgct ggtatgctca atgcaattgg cttgcaaaat cctggtttag aggttgtttt
    17401 ggctgaaaag ctaccttggc tggaaagaga atatccaaat cttcctatta ttgccaatgt
    17461 agctggtttt tcaaaacaag agtatgcagc tgtttctcat gggatttcca aggcaactaa
    17521 tgtaaaagct atcgagctca atatttcttg tcccaatgtt gaccactgta atcatggact
    17581 tttgattggt caagatccag atttggctta tgatgtggtg aaagcagctg tggaagcctc
    17641 agaagtgcca gtttatgtca aattaacccc gagtgtgacc gatatcgtta ctgtcgcaaa
    17701 agctgcagaa gatgcgggag caagtggctt gaccatgatc aatactctgg ttggaatgcg
    17761 ctttgacctc aaaaccagaa aaccaatctt ggccaatgga acaggtggaa tgtcaggtcc
    17821 agcagtcttt ccagtagccc tcaaactcat ccgccaagta gcccaaacaa cagacctgcc
    17881 tatcattgga atggggggag tggattcggc tgaagctgcc ctagaaatgt atctggctgg
    17941 ggcatctgct atcggagttg gaacagctaa ctttaccaat ccttatgcct gccctgacat
    18001 catcgaaaat ttaccaaaag tcatggataa atacggtatt agcagtctgg aagaactccg
    18061 tcaggaagta aaagagtctc tgaggtaaac tgcaatcaat ctgttcttga ttttttatta
    18121 gtttgtaata tgaatttagg agaattttgg tacaataaaa taaataagaa cagaggaaga
    18181 aggttaatga agaaagtaag atttattttt ttagctctgc tatttttctt agctagtcca
    18241 gagggtgcaa tggctagtga tggtacttgg caaggaaaac agtatctgaa agaagatggc
    18301 agtcaagcag cgaatgagtg ggtttttgat actcattatc aatcttggtt ctatataaaa
    18361 gcagatgcta actatgctga aaatgaatgg ctaaagcaag gtgacgacta tttttacctc
    18421 aaatctggtg gctatatggc caaatcagaa tgggtagaag acaagggagc cttttattat
    18481 cttgaccaag atggaaagat gaaaagaaat gcttgggtag gaacttccta tgttggtgca
    18541 acaggtgcca aagtaataga agactgggtc tatgattctc aatacgatgc ttggttttat
    18601 atcaaagcag atggacagca cgcagagaaa gaatggctcc aaattaaagg gaaggactat
    18661 tatttcaaat ccggtggtta tctactaaca agtcagtgga ttaatcaagc ttatgtgaat
    18721 gctagtggtg ccaaagtaca gcaaggttgg ctttttgaca aacaatacca atcttggttt
    18781 tacataaaag aaaatggaaa ctatgctgat aaagaatgga ttttcgagaa tggtcactat
    18841 tattatctaa aatctggtgg ctacatggca gccaatgaat ggatttggga taaggaatct
    18901 tggttttatc tcaaatttga tgggaaaata gctgaaaaag aatgggtcta cgattctcat
    18961 agtcaagcct ggtactactt caaatccggt ggttacatga cagccaatga atggatttgg
    19021 gataaggaat cttggtttta cctcaagtct gatggcaaaa tggctgaaaa agaatgggtc
    19081 tacgattctc atagtcaagc ctggtactac ttcaaatctg gtggttacat ggcgaaaaat
    19141 gagacagtag atggttatca gcttggaagc gatggtaaat ggcttggagg aaaagctaca
    19201 aatgaaaatg ctgcttacta tcaagtagtg cctgttacag ccaatgttta tgattcagat
    19261 ggtgaaaagc tttcctatat atcgcaaggt agtgtcgtat ggctagataa ggatagaaaa
    19321 agtgatgaca agcgcttggc tattactatt tctggtttgt caggctatat gaaaacagaa
    19381 gatttacaag cgctagatgc tagtaaggac tttatccctt attatgagag tgatggccac
    19441 cgtttttatc actatgtggc tcagaatgct agtatcccag tagcttctca tctttctgat
    19501 atggaagtag gcaagaaata ttattcggca gatggcctgc attttgatgg ttttaagctt
    19561 gagaatccct tccttttcaa agatttaaca gaggctacaa actacagtgc tgaagaattg
    19621 gataaggtat ttagtttgct aaacattaac aatagccttt tggagaacaa gggcgctact
    19681 tttaaggaag ccgaagaaca ttaccatatc aatgctcttt atctccttgc ccatagtgcc
    19741 ctagaaagta actggggaag aagtaaaatt gccaaagata agaataattt ctttggcatt
    19801 acagcctatg atacgacccc ttacctttct gctaagacat ttgatgatgt ggataaggga
    19861 attttaggtg caaccaagtg gattaaggaa aattatatcg ataggggaag aactttcctt
    19921 ggaaacaagg cttctggtat gaatgtggaa tatgcttcag acccttattg gggcgaaaaa
    19981 attgctagtg tgatgatgaa aatcaatgag aagctaggtg gcaaagatta gtactataag
    20041 tgaatatgat ttgagtgaat agtaagttaa aaatcctgat ttcaagtaaa atcaggattt
    20101 tttcatggat gcaatttttt tggagtctgg tgtgacgcgg agggtctttt gtcctgtgta
    20161 agtgacaaag ccgggttttc caccagttgg tttattgagt tttttgactt caatcatatc
    20221 tacctgcacc agattcgaca ggcgcccttg agagaagtag gcagctaact ctgctgcgtc
    20281 tgtcttgact gcatcagatg ggtcaagatt tcctgagatg acaacatggc ttccaggaat
    20341 gtccttagca tggaaccaaa gttcctcctt gcgggccatt ttaaaggtca attcctcatt
    20401 ttgaagattg tttcgtccga catagatgat ggttttgcca tcgcttgcta gatattgttc
    20461 tagttttttg cgtttctgga ttttctcccg ttgtcttctg cggataaaac ctgtttgaat
    20521 caattcttca cggatttcag cgatttcttc cagtccagct tggttgagga cggtttctac
    20581 actttccaga tagagaatag tggctttggt ttcttcaatc aaatcagtca agtatttgac
    20641 agcttctttg agtttctgat accgtttaaa atagcgttgg gcattctggt tgggagtcag
    20701 agccttatca agcgcaatca tgataggttg gttggtatag tagttgtcta ggataacctg
    20761 gtcttggtcg ttaggcactt ggtggaggaa ggttgtcagc aattctcctt tttgacgaaa
    20821 ttcttcagcg ttgtctgtcg ccagtaactc tttttcctgt tttttgagtt tgtgtcggtt
    20881 tttctgaagt tcattttcaa cacgacgaat cagttcactg gcctgctgtt tgacgcggtc
    20941 gcgctcagcc ttatccttat agtaggtgtc caacaaatca gaaagatttg caagaggctc
    21001 tcccacctga tttgcaaaag gaactggact gaaggaagtc tcagtcaagc atggcttggt
    21061 ttcttgattg aaaaaatttc ggaaagcgga aagtttttca ctaaccagta tcctttccaa
    21121 ttcatttgcc gtatcgcgtc ccagaccttg aaagaggctt tgaagatttt ttgctgttag
    21181 ttcttgggtt tgcaggattt caaagagctt ttcatccttg atagtaaaag gattgagaga
    21241 ttttgtactt ggcggagcga tataggtcga tcctggaagt aaggtgcggt agctattttg
    21301 tgaaaagccg acgtgtttga taacttcgag gattttatga ctgcttttat cgaccagtag
    21361 aatattactg tgtttcccca taatttcgat aatcaaggta gcctggatat ggtctccaat
    21421 ctcgttttta ttggaaactg taatttccac aatacggtca ttttccactt gctcaatcga
    21481 ctcaatcagg gccccctgca aatactttct caaaaccatg ataaaggtag aaggttgggc
    21541 tggattttca aaagtcgttt gggtcagctg aatgcgtcca aaaactggat gggcagaaag
    21601 gagcaggcga tggctttggc gattgctgcg gatttgcaag accaactctt gttcaaaagg
    21661 ctgattgatt ttctggatgc gaccattcac taattcgctt cgcaattcct caactatgtg
    21721 gtgtaaaaaa aatccgtcaa atgacatcgt tctctccttg tgattgtatt ccatagtatt
    21781 atatcaaaaa ggtagaataa aatcatggaa atgtggtata ataaagccaa gtaaagagaa
    21841 acgagaagca catgtatatt gaaatggtag atgaaactgg tcaagtttca aaagaaatgt
    21901 tgcaacaaac ccaagaaatt ttggaatttg cagcccaaaa attaggaaaa gaagacaagg
    21961 agatggcagt cacttttgtg accaatgagc gtagtcatga acttaatctg gggtaccgtg
    22021 acaccgaccg tccgacagat gtcatcagcc ttgagtataa accagaattg gaaattgcct
    22081 ttgacgaaga ggatttgctt gaaaatccag aattggcaga gatgatgtct gagtttgatg
    22141 cctatattgg ggaattgttc atctctatcg ataaggctca tgagcaggcc gaagaatatg
    22201 gtcacagctt tgagcgtgag atgggcttct tggcagtaca cggcttttta catattaacg
    22261 gctatgatca ctatactccg gaagaagaag cggagatgtt cggtttacaa gaagaaattt
    22321 tgacagccta tggactcaca agacaataaa cgaaaatgga aaaatcgtga cctgatatcc
    22381 agtttagaat ttgccatcac agggattttt actgctatca aggaagaacg caatatgcga
    22441 aaacatgcag tgacggctct agtggtcatc cttgcaggtt ttggttttca ggtgtcacga
    22501 atcgaatggc tctttctcct attgagcatt ttcttggtgg tagcctttga aattatcaat
    22561 tctgctatcg aaaatgtggt ggatttggcc agtcactatc acttttccat gctggctaaa
    22621 aatgccaagg atatggcggc tggcgcggta ttagtggttt ctctttttgc agccttaaca
    22681 ggcgcattga tttttctccc acgaatctgg gatttattat tttaaacagt aagaggaaat
    22741 tatgactttt aaatcaggct ttgtagccat tttaggacgt cccaatgttg ggaagtcaac
    22801 ctttttaaat cacgttatgg ggcaaaagat tgccatcatg agtgacaagg cgcagacaac
    22861 gcgcaataaa atcatgggaa tttacacgac tgataaggag caaattgtct ttatcgacac
    22921 accagggatt cacaaaccta aaacagctct cggagatttc atggttgagt ctgcctacag
    22981 tacccttcgc gaagtggaca ctgttctttt catggtgcct gctgatgaag cgcgtggtaa
    23041 gggggacgat atgattatcg agcgtctcaa ggctgccaag gttcctgtga ttttggtggt
    23101 gaataaaatc gataaggtcc atccagacca gctcttgtct cagattgatg acttccgtaa
    23161 tcaaatggac tttaaggaaa ttgttccaat ctcagccctt cagggaaata acgtgtctcg
    23221 tctagtggat attttgagtg aaaatctgga tgaaggtttc caatatttcc cgtctgatca
    23281 aatcacagac catccagacc gtttcttagt ttcagaaatg gttcgcgaga aagtcttgca
    23341 cctaactcgt gaagagattc cgcattctgt agcagtagtt gttgactcta tgaaacgaga
    23401 cgaagagaca gacaaggttc acatccgtgc aaccatcatg gtcgagcgcg atagccaaaa
    23461 agggattatc atcggtaaag gtggcgctat gcttaagaaa atcggtagca tggcccgtcg
    23521 tgatatcgaa ctcatgctag gagacaaggt cttcctagaa acctgggtca aggtcaagaa
    23581 aaactggcgc gataaaaagc tagatttggc tgactttggc tataatgaaa gagaatacta
    23641 agtagaggtg ggctcatgcc tgcttcttgt ttttacagaa ggaggactta tgcctgaatt
    23701 acctgaggtt gaaaccgttt gtcgttgctt agaaaaattg attataggaa agaagatttc
    23761 gagtatagaa attcgctacc ccaagatgat taagacggat ttggaagagt ttcaaaggga
    23821 attgcctagt cagattatcg agtcaatggg acgtcgtgga aaatatttgc ttttttatct
    23881 gacagacaag gttttgattt cccatttgcg gatggagggc aagtattttt actatccaga
    23941 ccaaggacct gaacgcaagc atgcccatgt tttctttcat tttgaagatg gtggcacgct
    24001 tgtttatgag gatgttcgca agtttggaac catggaactc ttggtgcctg accttttaga
    24061 cgcctacttt atttctaaaa aattaggtcc tgaaccaagc gaacaagact ttgatttaca
    24121 ggtctttcaa tctgcccttg ccaagtccaa aaagcctatc aaatcccatc tcctagacca
    24181 gaccttggta gctggacttg gcaatatcta tgtggatgag gttctctggc gagctcaggt
    24241 tcatccagct agatcttccc agactttgac agcagaagaa gcgacagcta ttcatgacca
    24301 aaccattgct gttttgagcc aggctgttga aaaaggcggt tccaccattc ggacctatac
    24361 caatgccttt ggggaagatg gaagcatgca ggattttcat caggtctatg ataagactgg
    24421 tcaaggatgt gtacgctgtg gtaccatcat tgagaaaatt caactaggtg gacgtggaac
    24481 ccacttttgt ccaaactgtc aaaggaggga ctgatgggaa aaatcatcgg aatcactggg
    24541 ggaattgcct ctggtaagtc aactgtgaca aattttctaa gacagcaagg ctttcaagta
    24601 gtggatgccg acgcagtcgt ccaccaacta cagaaacctg gtggtcgtct gtttgaggct
    24661 ctagtacagc acttttggca agaaatcatt cttgaaaacg gagaactcaa tcgccctctc
    24721 ctagctagtc tcatcttttc aaatcctgat gaacgagaat ggtctaagca aattcaaggg
    24781 gagattatcc gtgaggaact ggctactttg agagaacagt tggctcagac agaagagatt
    24841 ttcttcatgg atattcccct actttttgag caggactaca gcgattggtt tgctgagact
    24901 tggttggtct atgtggaccg agatgcccaa gtagaacgct taatgaaaag ggaccagttg
    24961 tccaaagatg aagctgagtc tcgtctggca gcccagtggc ctttagaaaa aaagaaagat
    25021 ttggccagcc aggttcttga taataatggc aatcagaacc agcttcttaa tcaagtgcat
    25081 atccttcttg agggaggtag gcaagatgac agagattaac tggaaggata atctgcgcat
    25141 tgcctggttt ggtaattttc tgacaggagc cagtatttct ttggttgtac cttttatgcc
    25201 tatcttcgtg gaaaatctag gtgtagggag tgagcaagtc gctttttatg caggcttagc
    25261 aatttctgtc tctgctattt ccgcggcgct cttttctcct atttggggta ttcttgctga
    25321 caaatacggc cgaaaaccca tgatgattcg ggcaggtctt gctatgacta tcactatggg
    25381 aggcttggcc tttgtcccaa atatctattg gttaatcttt cttcgtttac taaacggtgt
    25441 atttgcaggt tttgttccta atgcaacggc actgatagcc agtcaggttc caaaggagaa
    25501 atcaggctct gccttaggta ctttgtctac aggcgtagtt gcaggtactc taactggtcc
    25561 ctttattggt ggctttatcg cagaattatt tggcattcgt acagttttct tactggttgg
    25621 tagttttcta tttttagctg ctattttgac tatttgcttt atcaaggaag attttcaacc
    25681 agtagccaag gaaaaggcta ttccaacaaa ggaattattt acctcggtta aatatcccta
    25741 tcttttgctc aatctctttt taaccagttt tgtcatccaa ttttcagctc aatcgattgg
    25801 ccctattttg gctctttatg tacgcgattt agggcagaca gagaatcttc tttttgtctc
    25861 tggtttgatt gtgtccagta tgggcttttc cagcatgatg agtgcaggag tcatgggcaa
    25921 gctaggtgac aaggtgggca atcatcgtct cttggttgtc gcccagtttt attcagtcat
    25981 catctatctc ctctgtgcca atgcctctag cccccttcaa ctaggactct atcgtttcct
    26041 ctttggattg ggaaccggtg ccttgattcc cggggttaat gccctactca gcaaaatgac
    26101 tcccaaagcc ggcatttcga gggtctttgc cttcaatcag gtattctttt atctgggagg
    26161 tgttgttggt cccatggcag gttctgcagt agcaggtcaa tttggctacc atgctgtctt
    26221 ttatgcgacg agcctttgtg ttgcctttag ttgtctcttt aacctgattc aatttcgaac
    26281 attattaaaa gtaaaggaaa tctagtgcga gtaaaaatta atctcaaatg ctcctcttgt
    26341 ggcagtatca attacctaac cagtaaaaat tcaaaaaccc atccagacaa gattgaggtt
    26401 ttaaagtatt gtcccaagga aagaaaagta accctacatc ttgaatctaa gtagatttta
    26461 tggtaaaata atagggattt taaggagttt gatatgtata acctattatt aaccatttta
    26521 ttagtattat ctgttgtgat tgtgattgca attttcatgc aaccaaccaa aaaccaatcc
    26581 agcaatgtat ttgatgccag ttcaggtgat ttgtttgaac gtagtaaagc tcgcggtttt
    26641 gaagctgtaa tgcagcgttt gacagggatt ttagtctttt tctggctagc cattgcctta
    26701 gcattgacgg tattatcaag tagataagaa aataatgggc aggactaggt ctttgcctct
    26761 ttttattttt aaaggatgtt tgagaaggtt ttacagtaaa agaaaattaa aaaatctaga
    26821 aagaaaatat gaaagataga ataaaagaat atttacaaga caagggaaag gtgactgtta
    26881 atgatttggc tcaggctttg ggaaaagaca gttccaagga ttttcgtgag ttgattaaaa
    26941 ccttgtcctt aatggaaaga aagcaccaaa ttcgttttga agaagatggt agtctgacat
    27001 tagaaattaa gaaaaaacat gagattaccc tcaaggggat ttttcatgcc cataaaaatg
    27061 gctttggctt tgttagtctg gaaggcgagg aggacgacct ttttgtaggg aaaaatgatg
    27121 tcaactatgc tattgatggt gataccgtcg aggtagtgat taagaaagtc gctgaccgca
    27181 ataagggaac agcagcagaa gccaaaatta ttgatatcct agaacacagt ttgacaacag
    27241 ttgtcgggca aatcgttctg gatcaggaaa aacctaagta tgctggctat attcgttcaa
    27301 aaaatcagaa aatcagtcaa ccgatttatg ttaagaaacc agccctaaaa ttagaaggaa
    27361 cagaagttct caaagtcttt atcgataaat acccaagcaa gaaacatgat ttctttgtcg
    27421 cgagtgttct cgatgtagtg ggacactcaa cggatgtcgg aattgatgtt cttgaggtct
    27481 tggaatcaat ggacattgta tccgagtttc cagaagctgt tgttaaggaa gcagaaagtg
    27541 tgcctgatgc tccgtctcaa aaggatatgg aaggtcgtct ggatctaaga gatgaaatta
    27601 cctttaccat tgacggtgcg gatgccaagg acttggacga tgcagtgcat atcaaggctc
    27661 tgaaaaatgg caatctggag cttggggttc acatcgcaga tgtttcttat tatgtgaccg
    27721 aggggtctgc ccttgacaag gaagccctta accgtgcgac ttctgtttac gtgacagacc
    27781 gagtggtgcc aatgcttcca gaacgactat caaatggcat ctgctctctc aatccccaag
    27841 ttgaccgcct gacccagtct gctattatgg agattgataa acatggtcgt gtggtcaact
    27901 ataccattac acaaacagtt atcaagacca gttttcgtat gacctatagc gatgtcaatg
    27961 atatcctagc tggcgatgaa gaaaagagaa aagaatatca taaaattgta tcaagtatcg
    28021 aactcatggc caagcttcat gaaactttag aaaacatgcg tgtgaaacgt ggagctctca
    28081 attttgatac caatgaagcg aagattttag tggataaaca aggtaagcct gttgatatcg
    28141 ttcttcggca gcgtggtatt gccgagcgga tgattgagtc ttttatgttg atggctaatg
    28201 aaacagttgc cgaacatttc agcaagttgg atttgccttt tatctatcga attcacgagg
    28261 agcctaaggc tgaaaaggtt cagaagttta ttgattatgc ttcgagtttt ggcttgcgca
    28321 tttatggaac tgccagtgag attagtcagg aggcacttca agacatcatg cgtgctgttg
    28381 agggagaacc ttatgcagat gtattgtcca tgatgcttct tcgctctatg cagcaggctc
    28441 gttattcgga gcacaatcac ggccactatg gactagctgc tgactattat actcacttta
    28501 ccagtccaat tcgtcgttat ccagaccttc ttgttcaccg tatgattcgg gattacggcc
    28561 gttctaagga aatagcagag cattttgaac aagtgattcc agagattgcg acccagtctt
    28621 ccaaccgtga acgtcgtgcc atagaagctg agcgtgaagt cgaagccatg aaaaaggctg
    28681 agtatatgga agaatacgtg ggtgaagagt atgatgcagt tgtatcaagt attgtcaaat
    28741 tcggtctctt tgtcgaattg ccaaacacag ttgaaggctt gattcacatc actaatctac
    28801 ctgaatttta tcatttcaat gagcgtgatt tgactcttcg tggagaaaaa tcaggtatca
    28861 ctttccgagt gggtcagcag atccgtatcc gtgttgaaag agcggataaa atgactggag
    28921 agattgattt ttcattcgta cctagtgagt ttgatgtgat tgaaaaaggc ttgaaacagt
    28981 ctagtcatag tggcagaggg cgtggttcaa atcgtcgttc ggataagaag gaagacaaga
    29041 gaaaatcagg acgctcaaat gataagcgta agcattcaca aaaagacaag aagaaaaaag
    29101 gaaagaaacc tttttacaag gaagtagcta agaaaggagc caagcatggc aaagggcgag
    29161 ggaaaggtcg tcgcacaaaa taaaaaggca cgccacgact atacaatcgt agatacgcta
    29221 gaggcaggga tggtcctgac tggaactgaa atcaagagtg tacgagctgc tcgaattaat
    29281 atcaaggatg gctttgctca agtgaaaaat ggagaagttt ggctgagcaa tgttcatatc
    29341 gcgccttacg aagagggcaa tatctggaac caggaaccag aacgtcgtcg taaactcctg
    29401 ctccataaaa agcaaattca aaaattggaa caagagacca aagggacagg aatgacctta
    29461 gttcccctta aggtctatat aaaagatggc tacgctaagc ttcttttagg acttgccaaa
    29521 gggaagcatg actatgacaa acgggagtct atcaaacgtc gtgagcaaaa tcgagatatc
    29581 gcgcgtgtga tgaaagctgt taatcagcga taaaaagagg aattgaaaat ggaaaaatta
    29641 gttgcctata aacgcatgcc tttgtggaat aaacaaacaa tgcctgaaga tgttcagcaa
    29701 aagcacaata caaaagttgg gacttggggg aaaattactg tcttgaaggg agctctcaag
    29761 tttattgaat tgacagaaga aggggaagtt ctagctgaac acctctttga agcaggggca
    29821 gacaatccaa tggcccaacc tcaagcctgg caccgagtgg aagctgccac agatgatgtg
    29881 gaatggtact tggaatttta ttgtaaacct gaggattatt ttgctaaaaa atacaatacc
    29941 aatcctgttc attcagaggt cctagaggcc atgcagacag tgaaacaagg gaaagctttg
    30001 gatttgggtt gtggtcaggg gcgtaattct ctttttctag cccagcaaga ttttgatgtg
    30061 acggctgtag atcaaaatgg actagctctt gaaatcttgc aaagcattgt ggagcaggaa
    30121 gatttggaca tgcctgttgg cctttacgat atcaattcag ctagcattga acaagaatat
    30181 gattttatcg tttcaacagt tgttctcatg tttctacaag cggaccgcat tccagctatt
    30241 attcaaaata tgcaggagaa aaccagtgtt ggtggttaca accttatcgt ttgtgccatg
    30301 gacacggagg attatccttg ctcggttaat ttcccattca cctttaaaga aggagaactg
    30361 gcagactatt acaaggattg ggaattggtt aagtacaatg aaaatccagg ccatttgcac
    30421 cgtcgcgatg agaatggcaa tcgtattcaa ctacgctttg cgaccttact agctaagaaa
    30481 atcaagtaaa cacacatgaa gattaggaat tttcctgatc ttttttcttt tttacgaatg
    30541 atatagaaaa ggagggaatt catgtttgtt gcgagagatg ctaggggaga attggtaaat
    30601 gtgttagagg ataaacttga gaagcaagca tacacctgcc cagcttgtgg aggccagctc
    30661 catttgcgtc aaggaccaag tgtacggacg cattttgccc ataaatcctt aaaagactgt
    30721 gattttttct ttgaaaatga aagtccagaa cacctggcca ataaggaatc cctctatcac
    30781 tggttgaaaa aagagacaaa ggttcaatta gagtacccgc tttcagaact taaacagatt
    30841 gcggatgtat ttgtaaatgg caatctagct ctagaagttc agtgtagtcc cttgcctcag
    30901 aaagtcctta aagagcgaag tgagggatat cgtagtcagg gttaccaagt actgtggttg
    30961 ctgggtcaaa aactgtggct caaggagcgt ttgactcgtc tacagcaagg ttttctttat
    31021 ttcagtcaaa acatgggctt ttatgtttgg gaattagaca aggaaaaaca agttttaaga
    31081 ctcaaatacc tgatttacca ggatctccgc ggtaaactcc attatcaaat caaggaattt
    31141 tcctatggtc aaggtagttt attggaaata ttgcgtcttc cctataagaa acaaaaaata
    31201 tctcatttta cagtttctga ggacaaggac atctgtcgct atatccggca acaactttat
    31261 tatcaaaatc tcttttggat gaaagaacaa gcagaagcct atcaaaaggg agaaaatatc
    31321 ctgacttatg gactgaaaga atggtatcca caaattcgac caatagtggg caaatttttc
    31381 cagattgaac aagacttgac tagctattat cagcactttt atacctatta ccaaaaaaat
    31441 cctcaaaatg attggcaaaa gctttatcca ccagcctttt atcagcaata tttcttgaaa
    31501 aatatggtag aatagaaagg atggaggaat ctaatggtat tacaaagaaa tgaaataaat
    31561 gaaaaagata catgggatct atcaacgatc tacccaactg accaggcttg ggaagaagtc
    31621 ttaaaagatt taacagaaca attggagaca gtagcccagt atgaaggcca tctcttggat
    31681 agtgcggata acctactaga aatcactgaa ttttctcttg aaatggaacg ccagatggag
    31741 aagctttacg tttatgctca tatgaagaat gaccaggata cacgtgaagc taagtatcaa
    31801 gagtactatg ccaaggccat gacattctac agccagttag accaagcctt ttcattctat
    31861 gagcctgaat ttatggagat tagcgaaaag cagtatgctg actttttaga agctcaacca
    31921 aagctgcagg tttatcaaca ctattttgac aagctcttgc aaggcaagga tcacgttctc
    31981 tcacaacgtg aagaagaatt attggctgga gctggagaaa tctttggttc agcaagtgaa
    32041 accttcgcta tcttggacaa tgcggatatt gtgttccctt atgtcctaga cgatgatggt
    32101 aaagaagttc agctatctca tgggatttac acacgtttga tggagtctaa aaaacgtgag
    32161 gttcgccgtg gtgcctatca agctctttat gcgacttacg aacaattcca acacacctat
    32221 gccaaaacct tgcaaaccaa tgttaaggtg caaaattacc gtgctaaagt tcgtaactac
    32281 aagagtgctc gtcatgcagc cctcgcagcg aattttgttc cagaaagtgt ttatgacaat
    32341 ttggtagcag cagttcgcaa gcatttgcca ctcttacatc gctatcttga gcttcgttca
    32401 aaaatcttgg ggatttcaga tctcaagatg tacgatgtct acacaccgct ttcatctgtt
    32461 gaatacagtt ttacctacca agaagccttg aaaaaagcag aagatgcttt ggcagtcttg
    32521 ggtgaggatt acttgagccg tgttaaacgt gccttcagcg agcgttggat tgatgtttac
    32581 gaaaatcaag gcaagcgttc aggtgcctac tctggtggtt cttacgatac caatgccttt
    32641 atgcttctca actggcagga caatctggac aatctcttta ctcttgttca tgaaacaggt
    32701 cacagtatgc attcaagcta tactcgtgaa actcagcctt atgtttacgg agattactct
    32761 atctttttgg ctgagattgc ctcaactacc aatgaaaata tcttgacgga gaaattattg
    32821 gaagaagtgg aagacgacgc aacacgcttt gctattctca ataacttcct agatggtttc
    32881 cgtggaacag ttttccgcca aactcaattt gctgagtttg aacacgccat tcaccaagca
    32941 gatcaaaatg gggaggtctt gacaagcgat ttcctaaata aactctacgc agacttgaac
    33001 caagagtatt atggtttgag taaggaagac aatcctgaaa tccaatacga gtgggctcgc
    33061 attccacact tctactataa ctactatgta tatcaatatt caactggctt tgcggccgcc
    33121 tcagccttgg ctgaaaaaat tgtccatggt agtcaagaag accgtgaccg ctatatcgac
    33181 tacctcaagg caggtaagtc ggactatcca cttaatgtca tgagaaaagc tggtgttgat
    33241 atggagaagg aagactacct caacgatgcc tttgcagtct ttgaacgccg tttaaatgag
    33301 tttgaagccc ttgttgaaaa attaggattg gcataaaatg gttgaatcgt atagtaagaa
    33361 tgctaaccat aacatgcgtc gtcctgtcgt caaagaagaa attgtagact tgatgcgtca
    33421 gcgtcaaaag caggtcacag gtttcttgaa agaattggaa aactttgccc gcaaggaaaa
    33481 tattcctatt attccccatg aaacggttgc ttatttccgt tttcttatgg aaaccatgca
    33541 gcctaaaaat attctggaaa ttgggacggc tatcggtttt tcagctctct tgatggctga
    33601 acatgcgcca aatgctaaga ttacaactat tgatcgtaat ccagaaatga ttggttttgc
    33661 caaggaaaat tttgcccagt ttgacagtcg caagcaaatc actctcctag agggagatgc
    33721 ggtggatgtc ttatctacac tgacagagtc ttatgatttc gtctttatgg attctgccaa
    33781 gtctaaatac atcgtctttc tgccagaaat cctcaaacat ttggaagttg gtggtgtggt
    33841 tgtcttggat gatatttttc aaggtggtga tgttgccaag gatattatgg aagtccgtcg
    33901 tggtcagcga accatttatc gaggccttca aaaattattt gatgcaacct tagacaatcc
    33961 agaactcacc gcaacattag tgcctttagg agatggtatt ctcatgcttc gtaaaaatgt
    34021 agcagatgtt caactgtctg aaagcgaatg attttcagaa aaatttaaga aaaaatagta
    34081 aaatagatag agtaacactt atctcaaagg agtagacatg aagaaaaaat tattggcagg
    34141 tgccatcaca ctattatcag tagcaacttt agcagcttgt tcgaaagggt cagaaggggc
    34201 agaccttatc agcatgaaag gggatgtcat cacagaacat caattttatg agcaagtgaa
    34261 aaacaaccct tcagcccaac aagtcttgtt aaatatgacc atccaaaaag tttttgaaaa
    34321 acaatatggc tcagagcttg atgataaaga ggttgatgat actattgccg aagaaaaaaa
    34381 acaatatggc gaaaactacc aacgtgtctt gtcacaagca ggtatgactc ttgaaacacg
    34441 taaagctcaa attcgtacaa gtaaattagt tgagttggca gttaagaagg tagcagaagc
    34501 tgaattgaca gatgaagcct ataagaaagc ctttgatgag tacactccag atgtaacggc
    34561 tcaaatcatc cgtcttaata atgaagataa ggccaaagaa gttctcgaaa aagccaaggc
    34621 agaaggtgct gattttgctc aattagccaa agataattca actgatgaaa aaacaaaaga
    34681 aaatggtgga gaaattacct ttgattctgc ttcaacagaa gtacctgagc aagtcaaaaa
    34741 agccgctttc gctttagatg tggatggtgt ttctgatgtg attacagcaa ctggcacaca
    34801 agcctacagt agccaatatt acattgtaaa actcactaag aaaacagaaa aatcatctaa
    34861 tattgatgac tacaaagaaa aattaaaaac tgttatcttg actcaaaaac aaaatgattc
    34921 aacatttgtt caaagcatta tcggaaaaga attgcaagca gccaatatca aggttaagga
    34981 ccaagccttc caaaatatct ttacccaata tatcggtggt ggagattcaa gctcaagcag
    35041 tagtacatca aacgaatagt ccaaatcaat gagtcaggga aaaaaactcg acttcaggaa
    35101 aaaatgaagc aaacattccc acaataaaac gcatagtaca aggtttgtac tgaccccaaa
    35161 agttagacaa ttaatttatc tgaaggattt agttctgtat tgcacagggc taagtccttt
    35221 tagttttacc ttaattctct tattgttgta ataatcaata tagtctataa tggcttgttc
    35281 caattgatta agtgatttaa atgttttctc atagccataa aacatttcgg attttaaaat
    35341 gccaaagaaa gattccatcc taccgttgtc ttggctgttt cctttgcgtg acatagatgc
    35401 ttgaattccc ttactctcta ggaagcgatg ataagaatcg tgttggtatt gccagccttg
    35461 gtcactatgg agaatcgtat tctcgtagtg cttctctttg aatgcctgtt ccaaca
//
LOCUS       DAVBCR010000016        34386 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCR010000016 DAVBCR010000000
VERSION     DAVBCR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.016"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..4825
                     /locus_tag="VQ508_000697"
     CDS             <1..4825
                     /locus_tag="VQ508_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2489732.1"
                     /translation="LTGAQGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQGEKGDR
                     GERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGF
                     DGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQ
                     GLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLV
                     STHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTP
                     EVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYID
                     KDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNT
                     QTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGE
                     RGLTGSKGEKGDQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPK
                     GEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQ
                     SPTITATRSVQNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHT
                     LTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYV
                     KNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKD
                     VLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTP
                     EVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHT
                     IVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDP
                     ANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQ
                     DGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAP
                     KIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNG
                     IDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSH
                     TITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKD
                     GKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTI
                     VITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEV
                     IDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSII
                     KDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGD
                     GSHTITIINSDGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVP
                     PTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTP
                     AQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(5062..5610)
                     /locus_tag="VQ508_000698"
     CDS             complement(5062..5610)
                     /locus_tag="VQ508_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2489733.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5931..6419
                     /locus_tag="VQ508_000699"
     CDS             5931..6419
                     /locus_tag="VQ508_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2489734.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6416..7603
                     /locus_tag="VQ508_000700"
     CDS             6416..7603
                     /locus_tag="VQ508_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2489735.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7596..7961
                     /locus_tag="VQ508_000701"
     CDS             7596..7961
                     /locus_tag="VQ508_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2489736.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7977..9323
                     /gene="asnS"
                     /locus_tag="VQ508_000702"
     CDS             7977..9323
                     /gene="asnS"
                     /locus_tag="VQ508_000702"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2489737.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9476..9766
                     /locus_tag="VQ508_000703"
     CDS             9476..9766
                     /locus_tag="VQ508_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2489738.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9778..10248
                     /gene="ssbA"
                     /locus_tag="VQ508_000704"
     CDS             9778..10248
                     /gene="ssbA"
                     /locus_tag="VQ508_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2489739.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            10280..10519
                     /locus_tag="VQ508_000705"
     CDS             10280..10519
                     /locus_tag="VQ508_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2489740.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10814..12214
                     /locus_tag="VQ508_000706"
     CDS             10814..12214
                     /locus_tag="VQ508_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2489741.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12216..12575
                     /locus_tag="VQ508_000707"
     CDS             12216..12575
                     /locus_tag="VQ508_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2489742.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12629..13378
                     /locus_tag="VQ508_000708"
     CDS             12629..13378
                     /locus_tag="VQ508_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2489743.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13368..13667
                     /locus_tag="VQ508_000709"
     CDS             13368..13667
                     /locus_tag="VQ508_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2489744.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13738..14673
                     /locus_tag="VQ508_000710"
     CDS             13738..14673
                     /locus_tag="VQ508_000710"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2489745.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14760..15272
                     /locus_tag="VQ508_000711"
     CDS             14760..15272
                     /locus_tag="VQ508_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2489746.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15309..15904
                     /locus_tag="VQ508_000712"
                     /pseudo
     CDS             15309..15904
                     /locus_tag="VQ508_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            16005..16199
                     /locus_tag="VQ508_000713"
     CDS             16005..16199
                     /locus_tag="VQ508_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2489747.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(16245..17690)
                     /locus_tag="VQ508_000714"
     CDS             complement(16245..17690)
                     /locus_tag="VQ508_000714"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2489748.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17796..19418
                     /locus_tag="VQ508_000715"
     CDS             17796..19418
                     /locus_tag="VQ508_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2489749.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19622..21580
                     /locus_tag="VQ508_000716"
     CDS             19622..21580
                     /locus_tag="VQ508_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2489750.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21752..22687
                     /locus_tag="VQ508_000717"
     CDS             21752..22687
                     /locus_tag="VQ508_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489751.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            22712..23364
                     /locus_tag="VQ508_000718"
                     /pseudo
     CDS             22712..23364
                     /locus_tag="VQ508_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            23522..24616
                     /locus_tag="VQ508_000719"
     CDS             23522..24616
                     /locus_tag="VQ508_000719"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2489752.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24624..25790
                     /locus_tag="VQ508_000720"
     CDS             24624..25790
                     /locus_tag="VQ508_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2489753.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            25883..28981
                     /locus_tag="VQ508_000721"
     CDS             25883..28981
                     /locus_tag="VQ508_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2489754.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            29029..29646
                     /locus_tag="VQ508_000722"
     CDS             29029..29646
                     /locus_tag="VQ508_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2489755.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            29656..30990
                     /locus_tag="VQ508_000723"
     CDS             29656..30990
                     /locus_tag="VQ508_000723"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2489756.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            31000..31464
                     /locus_tag="VQ508_000724"
     CDS             31000..31464
                     /locus_tag="VQ508_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489757.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            31461..31955
                     /locus_tag="VQ508_000725"
     CDS             31461..31955
                     /locus_tag="VQ508_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489758.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            32036..33685
                     /gene="mltG"
                     /locus_tag="VQ508_000726"
     CDS             32036..33685
                     /gene="mltG"
                     /locus_tag="VQ508_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2489759.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33756..34238
                     /gene="greA"
                     /locus_tag="VQ508_000727"
     CDS             33756..34238
                     /gene="greA"
                     /locus_tag="VQ508_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2489760.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        10730 a         6378 c         7788 g         9490 t
ORIGIN      
        1 tctaactgga gctcaaggag ctaaaggtga aaaaggagcc caaggtgaac gcggtttaac
       61 tggagctaaa ggtgaaaaag gggaccaagg tgaacgcggt ctcactggag cccaaggtga
      121 aaaaggagac cgaggcgaac gtggtttaac tggagctaaa ggtgaaaaag gagcccaagg
      181 tcaggcaggt cgtgacggtg taactccaac cgtaaccgtt aaagataata aaaatgacgg
      241 cactcatact atcactatta acgacggtag aggtaatgtt acaagtactg ttgtaagaga
      301 tggtttcgat ggcgcaagtc cattagttgc gactcaacga aatgacgcag ataagacaac
      361 aactgttatc ttctattacg ataaaaatgg aaacaatgaa ttagatgctt ctgataagaa
      421 attaaaagaa gttgtaattg cagatggtgc taaaggtgaa aaaggggaca aaggtgaaca
      481 aggtcttcaa gggcgtgatg gtgaacaagg accaaaagga gaagatggga aaaccccaac
      541 agttaaagta actgatggtc aagatggaac gcatacaatt acaattaacg atggtaaagg
      601 tggtataact actacagtag taagagatgg atttgatggt gcaagccctc ttgtttctac
      661 tcatagaaat gaagcagata aaacaacaac tgttattttc tattatgatc taaatgataa
      721 taatcaattt gatgaaggag atacaaaact taaagaagtt gttatcgcag atggaaaaca
      781 aggaccaaaa ggtgacaaag gtgataacgg aaaagatgga ttcacaccag aagtaacagt
      841 tacagataac aataacggaa cacacaccat cacaatcaca caaccagaca acagaccatc
      901 attaacaaca atcgttaaaa acggtgaaga tggaaaaaca ccaaaagtca aagcagaacg
      961 agatgatgcg aagaaacaaa caacattaac attctacatt gacaaagatg gagatggaag
     1021 ttacacagca ggaaaagacg agttagttca aacaacagta gttaaagacg gacaagacgg
     1081 agctgcagga gcatctggac gtgatggaaa agaagtatta aacggaaaag tagacccaac
     1141 aacagaaggt aaagatggag acacattcgt aaatacacaa acaggagatg tattcgttaa
     1201 gaaaggtaac acttgggaac cagcaggaaa catcaaagga ccgaaaggtg acaaaggtgc
     1261 agatggtgcc aagggtgaaa aaggagccca aggagaacgc ggcctgactg gagcgcaagg
     1321 tgtcaagggt gaaaagggag atcagggtga acgcggtcta actggatcta aaggtgaaaa
     1381 aggagaccaa ggtgaacgcg gtttaactgg agcgcaaggt gccaaaggtg acaaaggtga
     1441 acaaggtctt caaggtcgtg atggagctca aggaccaaaa ggcgcagatg gacaaagagg
     1501 accagcggga ccacaaggac caaaaggaga acaaggtaat ccaggaactc caggtaaaga
     1561 tggaaaatct ctaattgctg ttaaaaatgg aactgaaaca aaagtctatg tagaggatcc
     1621 ggctagacca ggacaaccac ttaatccaaa ccaaccatta gcaactatca ctgatggaag
     1681 aaacggtact aatggacaat caccaacaat tacagcgaca cgaagtgtgc aaaatggcaa
     1741 aaatggtgta ttagtaacaa ttacaccagt aggaggtcgt ccacaaacaa catttgtaga
     1801 ggatggacaa aagggtgctg atgggaaaac cccaacagta acaataactg aggggcaaaa
     1861 cggcacacat acattaacgg ttcataatcc aggaagtcca gatgtgacaa ctacgatccg
     1921 tgatggagct acaggacaag caggtcgtga tggtaaagat gtattaaacg gaaaagtaaa
     1981 tccacaacca aaccaaggta aaaatggaga taaatatatt aataccgaaa ccggtgatgt
     2041 ctatgttaaa aacaatggaa actgggataa agaaggcaac atcaaaggcc ctaaagggga
     2101 caaaggtgca gatggtgcta aaggcgaaaa aggagaccaa ggcgaacgcg gcctaactgg
     2161 agcgcaagga gctaaaggtg cggatggcgc agtaggtcgt gatggacgtg acggtaaaga
     2221 cgtgttgaac ggcaaagcta acccagaagc acatcaaggt aaagacggcg ataaatacgt
     2281 taatacagaa acaggcgacg tcttcgttaa gaataacggc aactgggata aagagggcaa
     2341 catcaaaggc cctaagggtg acaaaggtga acgcggagaa gatggtaaga ctccagaagt
     2401 aactgtaact ccaggtaaag atggccatag tactgacatt acattcactg ttccaggtaa
     2461 agatccagtt acagtaaatg ttaaggacgg agaaaatggt ctgaacggta aaactccaaa
     2521 agttgattta cttcgtgtcc aaggaaaaaa cggaaatcca tctcatacaa ttgtgacatt
     2581 ctatacagat gaaaacaatg acggcaaata tacaccagga actgatgaac ttctaggttc
     2641 agaaatgatt aaagatggtg ctaaaggcgc ggacggacga gatggtaaat cattgcttac
     2701 tgtcaaggat ggtaaagaaa ctaaagttta ccaagaagat ccagctaacc caggacaacc
     2761 attaaatcca gaaaaaccac ttgcggtaat tagagatgga gtagatggaa aatcacctac
     2821 agttacagct gttcgtaaag atgaagcagg gcataaaggt gtagaaatca ctgttgataa
     2881 ccatgatggt tcacaaccaa ctacagtctt tgttcaagat ggtgctaagg gagaaactgg
     2941 tgcaaccggt caggatggac aaactcctac aatcactact caacgtggac aagatggcca
     3001 aagcactgtt gtaactatca caacatcagg taaagatcca gtaaccttca ctgtaaaaga
     3061 tggtaagaat ggtaaagatg gccgtgcacc gaaaatcaaa gtagaagata ttacttcacc
     3121 ttcaagaatt agacgcgata cagatgctgc tgcaactcca acgcgtaacg gtatccgtgt
     3181 tacagtttat gatgatgtta atgacaatgg ggtatacgac gaaggtgtcg ataaagtatt
     3241 aaatagtaaa gatatttata acggtataga tggacgtgat ggttcagctc caactattac
     3301 tacaaaagat aatggagatg gaactcacac tatcacagtt caaaacccag atggttctga
     3361 atcaacaaca gttgttaaag atggtaaaga cggtaaaact gcgaatatca ctacaacaga
     3421 aaacccagat ggaagccaca caattacggt gacaaatcca gatggttcaa ctaaagaaac
     3481 tgttgttaaa aacggtaaag acggtaagac tcctaaagtt gaagtaacgg ataacaacga
     3541 tggaactcat actgttaaag ttacagatgg agacggcaat gttaccaacg ctatcatcaa
     3601 agatggtaaa gacggtaaag ctgcaacagc aacaactact gaaaatccag atggaagcca
     3661 cacagtaaca atcactaacc cagacggaac taagaatgag tttgttgtta agaatggacg
     3721 tgacggtgtt gacggacgta ctccaaccgc atctgttcgt gataatggag acggaagtca
     3781 tacaatcgtt attacaaatc cagaaggtgt gacaactgaa accacagttc gtgatggtaa
     3841 atcaccaaaa gtgactataa ctgatgaaca aaatggaact cataagatct ctgttctaaa
     3901 tggtgacgga acaactacaa caactacagt tcgtgatggt agagaaccaa aacttgaagt
     3961 tattgataac aacgatggtt cacacactat taaagtgaca ggtgctgatg gtaaaggaac
     4021 gacaactaca atctttgatg gtaaatcacc aaaagcgaac atcgttgata acggagatgg
     4081 aactcataca ttaacaatcg tagattctga tggtcgtgaa tacaaatcta ttatcaaaga
     4141 tggtaaagac ggcaaagata gcgtttcacc aactgtaact gttaaaaata ataacgatgg
     4201 aactcacgtt gttacaatca ctaatccaga tggaagtaag acagaaatgg tgattaaaga
     4261 cggtaaagat ggtaaatcac caaaagtttc tgttgaagat aatggtgatg gtagtcatac
     4321 aatcacaatc atcaattctg atggaactgt gacaaaaaca gttattaaag atggtagaga
     4381 tggacgtgat ggtcgagacg gcaaagacgg taaagacggt aaagatggaa aatgtggatg
     4441 ccaagacaaa ccagtaacac catcaaatga caaaccagtt cctccaacac caaatgtgcc
     4501 gacaccagaa gtaccggtta aacctgtgcc agcgcaacca acaccaaatg taccgacacc
     4561 agaagtgcca gtacaaccaa ctccagctgt tccaacacca gaagtaccgg ttaaaccagt
     4621 accagcggtt ccagaacaac cagtagtacc aacaccggct caaccagcaa ctccagtaaa
     4681 tgctaaccca gtagcaccaa ctacaggtaa agaaaaccgt ggggacaaat tacctgaaac
     4741 tggaagccaa tctgattata tctctgttct tttaggtagc ggtattctat tgagcctata
     4801 tgtaggacga agaaaagaag cttaatcagc caaaagcagt cactaatttc tcgaaagaag
     4861 atgataagtg atagatctcg aatgaaagaa tattcttaga atgattttcc tatgatggaa
     4921 aagactgcta aaaaccttga gtggtaacgc tcaaggtttt tttggatggc tgatgtttgt
     4981 ctgaaaatct tgattgagaa ataaaaagcc accctattag gtggctataa ttgatttaga
     5041 ctttctaaaa ttttctattt tttactgaat cgcttcgacc aagtcttgag cttgtttttc
     5101 aagtgagttt aggactgttt cttcaagaac caattttccg tctgcccagg cagagtcatt
     5161 aacacgtgca gcagtgaaat caccaacgcc ttgtgtacgg ataaatggca agaggtcttt
     5221 gtagatagcg aaaagttgat cgtgccctgc attggctaca gatgatacgg tgacaaactt
     5281 gtcttgaagg gcagaaacgc cacgcgtatc agacaagtca agggcacgag atagccagtc
     5341 aagcaagttt tttactgtac cagggataga gaagttgtag actggagaga aaatccagat
     5401 agcatccgca acgagaactg cttcacgagc agcagctaca gctggatgtg ttggaacttc
     5461 caaatcttgg ctgaagagag gaagggctga ataatcaagg tagctaactt ccgctttacc
     5521 agcaagtgct ttctcagctt cgagcgccat ttggtggttg aaagaacctt gacgtagtga
     5581 tccgacgata aataatactt ttttagacat gatgtgtctc ctttttcttc aaattttgag
     5641 aactctctcg ttgttataaa atacattaca acaaaacaaa agaacttgca agaaatttgc
     5701 tcagagttta tttgaaaatt gtaaaatttc ggaaactcta gtcatttgtg actgaaaaag
     5761 taagaactgt ttcactttca aaatcggttt tgtttgaagg gagaaataga attggttgct
     5821 caaagcacag ctttgaggtt gcagataaag ctgacgtggt ttgaagagat tttcgaagag
     5881 tattcttata ttcacttgta ataaagctta atagatggta aaatagacga gtgaaaaaaa
     5941 gacaaaacaa agcaaaaaat aatctactgt ggcagtatgg tctagggatg acgattttgt
     6001 ttgtggttat cagtgcttcc tttctgtata tggtttctct tagcatgaaa ccctatcaaa
     6061 cagctaaaag tgaaggagaa aaattagctc agcagtatgc aggattagag caggccgatc
     6121 aggttgattt atacaatggc ttggaatctt attacagcgt tcttggtcgt aataaacagc
     6181 aagaagcact tgctgttctg attggaaaag atgatcataa gatttacgtt tatcagctaa
     6241 atcagggtgt ttcacaagaa aaagcagaaa cggtttctaa ggaaaaggga gctggcgaaa
     6301 ttgacaagat tatctttggt cgttatcaag ataagccaat ctgggaagtc aagtcaggat
     6361 ctgattttta tctagtagat tttgaaacag gagcattggt caacaaggag ggcctatgaa
     6421 attatccaac cgtgttttag aaatggaaga aagtgtgact ttggctgctg gagcccgtgc
     6481 caaagcactg aaggctgagg gtagagatat tttgtctcta accttgggtg agccagattt
     6541 cactactccc aaaaatatcc aagatgccgc cattgcatcg attcgagatg ggcgtgcttc
     6601 tttttataca gtaacctcag gtctgccaga gcttaaggcg gcggtcaata gctactttga
     6661 gcgcttttac ggctattctg tagcgtcaaa tcaagtgaca gtcgctgcgg gagccaaata
     6721 ttctctctat accttcttta tggctgtggt caatccaggt gatgaagtca tcatcccaac
     6781 cccatactgg gttagctatg gagatcaggt caagatggca gaaggcgttc ccgtctttgt
     6841 ttctgctaag gaagacaatc actttaaggt gaccgtagag cagttagaag cagctcgcac
     6901 tgacaagacc aaggttttgg tgctgaattc gccatctaat cccacaggta tgatttacac
     6961 ccgtgaggaa ctcttggcaa ttggaaactg ggctgtagaa aatgatattc tcatcctagc
     7021 agatgatatc tatggccgct tggtttataa tggtcatgag ttcacaccga tttctagcct
     7081 atcggaagcg attcgcaagc aaacagtggt catcaatggt gtgtctaaaa cctatgccat
     7141 gactggttgg cggattggtt atgccgttgg agaagcagac attatcgctg ccatgtccaa
     7201 gattgcaggt caaacaactt cgaatccgtc agcagtagcc caatatgcag cagttgaggc
     7261 tctatcaggt gagcaagata ctgtagaaag catgcgtcag gcctttgaag aacgtcttaa
     7321 taccatctat cccctccttg cagaagtgcc aggatttgaa gtggtcaaac cgcaaggggc
     7381 cttctatctc ttcccaaatg tcaaaaaggc catggagatg aaaggctaca cggatgtgac
     7441 agactttaca actgttatct tagaagaagc cgaagtggcc ttggtaacag gagctggatt
     7501 tggagcacca gaaaatgtcc gcctcagcta tgcgacagac ctagacacgc ttaaagaagc
     7561 agtcgaacgc ttgaaagcat ttatgggtag tgagaatgat tgatcatttt gagattaagg
     7621 taaaggattt acaaatttca gaaggatttt ataggagttt tctcgctcct ttggactata
     7681 aattggcttt taagactagt tctctaatta gttttctatc cccgaacagc cctcatcctg
     7741 gtggtgattt ttggctgact cagggaacac aagatcctgt tcattttgct tttttagcag
     7801 aaaataagga agaagttcag gcttgttatg aggctggctt agaggcaggt gggcgagaca
     7861 atggggctcc tggttatcga agtgagcatc cgatttacta tgctgctttt atgattgacc
     7921 tggatgggaa caatatagag gtggtttgcc ataaagaata aaaaagaaaa ggaaaaataa
     7981 tgacaaaacg tgtaacgatt attgacgtaa aagactatgt tggtcaggaa gtgacgattg
     8041 gcgcttgggt tgccaacaaa tcaggaaaag ggaaaatcgc tttcttacaa ttgcgtgatg
     8101 gaacagcctt ctttcaaggt gtggctttta aaccaaactt tgtcgaaaaa tttggtgaag
     8161 aagtgggact tgagaagttt gatgttatca aacgcttgag ccaagaaacg tctgtttatg
     8221 tgacaggtat tgtcaaagag gacgaacgtt ctaaatttgg ctatgagttg gacatcacag
     8281 acatcgaagt gatcggtgaa tctcaagact acccaatcac accaaaagaa cacggaacag
     8341 actttttgat ggataaccgt cacttgtggc tacgctctcg taagcaagta gctgtgttgc
     8401 aaatccgtaa cgctattatc tatgcaactt atgagttctt tgacaagaac ggttttatga
     8461 agtttgacag cccaattctt tcaggaaatg cggcagaaga ttctacagaa ctctttgaaa
     8521 ctgactactt cggaacgcca gcctacttga gccaatcagg tcaactttac ctagaagcag
     8581 gggctatggc tcttggtcgt gtctttgact ttggtccagt tttccgtgct gaaaaatcaa
     8641 aaacacgccg tcacttgact gagttctgga tgatggatgc tgagtactca tacttgacac
     8701 atgatgagtc gcttgacttg caagaagctt atgtgaaagc tcttctacaa ggtgttcttg
     8761 accgcgcgcc tcaagccttg gaaaccttgg aacgtgatac agaactcttg aaacgctaca
     8821 ttgcagagcc attcaaacgt atcacttacg atcaagccat tgacctcttg caagagcatg
     8881 aaaatgatga agatgctgac tacgagcatc ttgagcatgg tgatgacttt gggtcaccac
     8941 acgaaacttg gatttcaaac cactttggtg tgccaacatt tgtcatgaac tatccagcag
     9001 ccatcaaggc cttctacatg aaaccagttc ctggaaatcc agagcgcgtg ctttgtgcag
     9061 acttgcttgc tccagaaggc tatggagaaa ttatcggtgg gtctatgcgt gaggaagatt
     9121 acgatgccct tgtcgctaag atggatgaac ttggcatgga tcgtacagaa tatgaattct
     9181 accttgacct tcgtaaatac ggtacagttc cacacggagg atttggtatc ggtatcgaac
     9241 gtatggtaac cttcgcagca ggaacaaaac atatccgtga agctattcca ttcccacgta
     9301 tgttgcaccg tatcaaacca taagaacagg aaaacgccca tgtggcgttt tttcgaattc
     9361 tagtgttttt acttgcgtaa aaaaaatttt tctagtataa tagtttattg tgagcgaacc
     9421 tcacttaccc cttgcaaagt cttggggtca ttagaccaaa aggaggaaca tatcaatggc
     9481 taaatacgaa attctttata tcattcgtcc aaacattgaa gaagaagcta aaaacgcttt
     9541 ggtagcacgt tttgactcta ttttgactga caacggtgca actgttgttg aatcaaaaac
     9601 ttgggaaaaa cgtcgtcttg catacgaaat ccaagatttc cgtgaaggac tttaccacat
     9661 cgttaacgtt gaagcaaatg acgatgcagc tcttaaagag tttgaccgtc tttcaaaaat
     9721 caacgctgac attcttcgtc acatgatcgt caaaattgac gcgtaagaag gtaaactatg
     9781 attaacaatg ttgtacttgt agggcgtatg acacgtgacg ctgaattgcg ttatacccca
     9841 tcaaatgtag cagttgcgac ttttactctt gcagtaaacc gtacatttaa gagtcaaaat
     9901 ggtgaacgtg aggctgattt tatcaatgtc gttatgtggc gccaacaggc tgaaaatctt
     9961 gctaactggg ctaaaaaagg ctcacttatc ggggtgacag gtcgtatcca gactcgtagt
    10021 tacgataacc agcaaggaca acgtgtctac gtgacagagg tcgtggctga gaatttccaa
    10081 atgttggaaa gccgtagtgt gcgtgagggt cacacaggtg gagcttactc tgcaccaact
    10141 gcaaactatt cagcacctac aaattcagta ccagactttt cacgtaatga aaatccattt
    10201 ggagcaacaa acccattgga tatttcagat gatgatttac cattctaatg gacaactaaa
    10261 cttataaagg agaaaaaaca tggctcaaca acgtcgtggc ggattcaaac gccgtaaaaa
    10321 agttgattac atcgcagcaa acaaaattga atatgttgat tacaaagata ctgagcttct
    10381 tagccgtttc gtttcagaac gtgggaaaat ccttcctcgt cgtgtaacag gaacttcagc
    10441 taaaaaccaa cgtaaagtaa caacagctat caaacgcgct cgcgtaatgg ctttgatgcc
    10501 tttcgtaaac gaagattaaa gaaaagaccc ggttgggtct ctttttcatg aacttgaaaa
    10561 caagaaagcg aattaagacc ccaacgggtc ttttttcaca agctttgaaa tgagagagcg
    10621 agttaggctc agtggctccc tttttacgag tttgaaaaca agagaacaca ttaaaagcac
    10681 gatggagctt tttcataaat atgtcttttt tttcatccaa gaaattaaaa taaaaatgat
    10741 aaactttttg aaaatagggg atttgcctac tttatgatat aatggaaaga gttagattga
    10801 aagaggtagt gagatggacg cgaaattaag atacaaggca aagaagatca agattgtctt
    10861 ttttgatatt gatgatacat tgcggaattc aaagacaggg tttattccaa ctacaattcc
    10921 cactgttttt aaacagttgc gtgaaaaagg aattttaaca ggaatcgcct ctggacgtgg
    10981 catttttggt gttgttccag agattcgtga tctcaaacct gacttttttg taactttgaa
    11041 tggggcttat atcgaagata aaaaaggtca ggtcatttat cagcatcaga ttgaaaagtc
    11101 atatgttgag gagtatatct cttgggctaa gcaagaagga attgagtatg gcttggttgg
    11161 gagtcatgat gccaagttgt cgactcgcac cgatatgatg agtgaagcta tcaatccaat
    11221 ttatcccgac ttagatgtag atcccgattt ccatgaaaaa gaagatatct atcagatgtg
    11281 gacttttgaa gataagggag atgacttgca cttgcctgac agtctctcag acaaacttcg
    11341 catggttcgt tggcatcaac attcgtctga tattgtgccg atttcaggct ccaaagcgac
    11401 gggggtggaa aaggttgtgg aacaccttgg cttgaaacca gagaaggtca tggtttttgg
    11461 agatggtctc aatgacttgg aactctttga ttatgctgga atcagcgttg ccatgggaat
    11521 ttctcatgat aaaatcaaag aaaaagcaga ttatattaca aaaacattag aagaagatgg
    11581 catttttgat gccttagaag tatttggtat ggtagaaaaa gaattgcatt ttccacaagt
    11641 agacattgaa acagtagaag gtcctcttgc gactattaag accaatcacg gagacttacg
    11701 tatcaagctt ttccctgaac atgctcctaa aacagtggct aactttgtat ctctttcaaa
    11761 agatggctac tatgatggtg tcattttcca ccgtattatc aaggacttta tgatccaagg
    11821 tggagaccca actggaactg gtatgggtgg cgagtcaatc tacggcgaat cttttgagga
    11881 tgaattctca gaagagcttt acaatatccg tggtgctctt tccatggcaa atgctggtcc
    11941 aaataccaac ggcagccagt tctttatcgt gcaaaaccaa cacctacctt attctaagaa
    12001 agaaattact cgtggtggtt ggccagaacc gattgcagaa atctatgcca atcaaggtgg
    12061 gacacctcac ctagaccgcc gtcacacggt ttttggtcag ttagctgatg aagcatctta
    12121 cgctgtcttg gatgtcattg ctgctgttga gacaggagct atggacaagc cagttgaaga
    12181 tgttgtaatt gaaactattg aaatcgagga ctaagatgaa aatcggtgat aagctaaagg
    12241 ggcgtattac agggattcag ccctacggtg cctttgttga gttagagacg ggtgatacgg
    12301 ggctgattca catttcagaa attcggacag gatttattga aaatattcat gaaaccttga
    12361 aagtcgatga agaggttcaa gttcaggtag tggatttaga tgaatttacg gggaaagcca
    12421 gtctttctat ccgcactttg gaggaagaaa agtaccagtt tcctagacgg agacgctttt
    12481 caagcgaccg ctttaactac ggttttgctc cctttcgacg aatgctacca atctggactg
    12541 gagaagccct gcaccattta aaaaagaaga agtagtttag agttttatat tttttgtaat
    12601 gttaatataa atttgctata ataggaccat ggaagaagaa caattattaa aatcaggaga
    12661 gcgcattaac cagctctttt cgacagatat caaaatcatt caaaatagag aggtttttag
    12721 ctattcggtg gatagtgttc tcttatcacg atttccacgt tttcctaaga aggggttgat
    12781 tgtggatttc tgtgctggga atggagcagt ggggcttttt gctagcactc gtactcaagc
    12841 acagatattg tctgttgaga ttcaggagcg tttggcggat atggctgaac gctctgtccg
    12901 tttgaatgga ttagaggagc aaatgcaggt catctgcgat gatttgaaaa atatgcctgc
    12961 tcacattcag gggagtaagg tggatatgat tttgtgtaat ccaccctatt tcaaggtgga
    13021 tccttattct aatctgaacg agagtgaaca ttatctcttg gctcgacacg aaatcacgac
    13081 taatttggaa gaaatctgtc atagtgccca gagtattctc aagtctaatg ggcgtttggc
    13141 catggttcat cgtcctgatc gacttctgga tattttggat acgttaaaac gacataatct
    13201 agcccctaag cgcctgcagt ttgtttatcc aaaaagagaa aaggaagcca atatgctttt
    13261 gattgaggcg atcaaggatg gctcaacaag tggctttaag gtcttacctc ctttgattgt
    13321 ccacaatgat gatggctctt atacgcccga aatcgaagag atttattatg gatcataagg
    13381 cttatatgta cgttctggag tgtcgtgatg gatcctacta tataggctat acgactgata
    13441 tgagaagacg cctcgctatc cacaatagtg ggaagggagc caaatataca cgagcacgct
    13501 tgccagtcaa acttatctat gctcaaggtt ttgccagtaa ggaagaagcc atgtcggctg
    13561 aagctctttt caagcgtaag aagaggccac agaaggaaga atttttatct gaaaatcaag
    13621 atagaaattt actcagttat tttgaagaat cgtggggagt cctttgactc ctcttacctt
    13681 tgtcaaaaag cgaaaaaaat gctacaataa agaaaataaa caaaatgagg tattatcatg
    13741 tccaagattc tagtatttgg tcaccaaaat ccagactcag atgccatcgg gtcatctgta
    13801 gcttttgcct accttgcaaa agaagcttac ggtttggata cggaagctgt tgcccttgga
    13861 actccaaatg aagaaacagc ctttgtcttg aactattttg gtgtggaagc accacgtgtt
    13921 atcacttctg ccaaagcaga gggggcagag caagttatct tgactgacca caatgaattc
    13981 caacaatctg tatcagatat cgctgaagta gaagtttacg gtgttgtaga ccaccaccgt
    14041 gtggctaact ttgaaactgc aagcccactt tacatgcgtt tggagccagt tggatcagcg
    14101 tcttcaatcg tttaccgtat gttcaaagaa catggtgtag ctgtgcctaa agagattgca
    14161 ggtttgatgc tttcaggttt gatttcagat acccttcttt tgaaatcacc aacaacacac
    14221 ccaacagata aaatcattgc tcctgaattg gctgaattgg ctggtgtaaa cttggaagaa
    14281 tatggtttgg caatgttgaa agctggtacc aacttggcta gcaaatctgc tgaagaattg
    14341 attgacatcg atgctaagac ttttgaactc aacggaaata atgtccgtgt tgcccaagtg
    14401 aacacagttg acatcgctga agttttggaa cgccaagcag aaattgaagc tgcaatgcaa
    14461 gctgccaacg aatcaaacgg ctactctgac tttgtcttga tgattacaga tatcgtcaac
    14521 tcaaactcag aaatattggc tcttggtgcc aatatggaca aggtcgaagc ggctttcaat
    14581 ttcaaacttg aaaacaatca tgccttcctt gctggtgccg tttcacgtaa gaaacaagtg
    14641 gtacctcaat taactgaaag ctttaatgcg taagattttg ggtgtcagct caaaatcgga
    14701 aagtctagtt tgccttatat cgaaaggagt ttcggctcct ttttttctag gagtgaagta
    14761 tgttagaaaa tggcgatttg atttttgtga gagatgggtc agacatggga caggccatcc
    14821 agacttccac aggtaactat agccatgttg ccatttattt ggatgggatg atttatcatg
    14881 ctagtggaca ggctggtgct gtctgtcaag aaccggcaga cttctttgag tccaatcatt
    14941 tgtacgacct ctatgtttac ccagaaatgg atatccagtc ggtgaaggaa agagcttgca
    15001 gacatcttgg agcaccctac aatgcttctt tctatccaga tgcagctggt ttctactgct
    15061 cccagtatat agcagaaatc ctacctattt ttgaaactat tcctatgaaa tttggagatg
    15121 gggaacagga gattagtgat ttttggaggg agtattacat agaactaggt ctgcctgttc
    15181 ctctgaacca agctgggacc aatcctagtc agttggcagc atcgcctctg ttacaatgta
    15241 aagaaaggaa tcttcatgat tcagattttt aattcatctc gtttgacgag acagccattt
    15301 ttggagaatt gatccgctat ctggaccagt atgaggatgt gattctacgg gaaattaagg
    15361 ctcaatttcc agatgttgca gttgataaac tcatggaaga gtatataaag gcaagcttga
    15421 ttctacgtga aaataagcgc tattacctca attttcctac gcttgaatca cttgatagtc
    15481 ttgaactgga tcaagagatt tttgtcagag aagctagtcc ggtctatcaa gccttgttgg
    15541 agcagagttt tgagacggaa ttgcgcaatc aaatcaatgc agctatttta gttgaaagac
    15601 ggactttgcg cgcattaaaa tgaccctgtc caattatttt tacaaggtca aacagcagta
    15661 tcctttgaca gaaaaacagc aggagctcta tgacatttta ggagatgtta atcctgagta
    15721 tgcactcaag tatatgacgg cttttttgtt gaaatttctc aaaaaagacc agcttatgca
    15781 gaaatgccgt gatatctttg tggacagttt ggttgtctta ggctatattg tgcaaaatga
    15841 agatagaaag tatgagttgg ctatcgattt tgataaggag agattaactt tctacttagc
    15901 gtgatttctt gtttctgagc acattgtttg actttcctta gtattcggta taaactatat
    15961 gtaaccggta acacatatcg gaataaacta aaggagacaa tcatatgtca cttgaaaaca
    16021 aattggaaca agcaacaggc gctgtcaaag aaggttttgg taaagttact ggagacagca
    16081 agacagaact tgaaggagct gttgaaaaaa cagttgctaa ggcaaaagac gttgtagaag
    16141 acgcaaaagg tgctgtagaa ggtgccgttg aaggtttgaa aaacgttttt actaaataat
    16201 aggaaaaaat caagggtttc attttccctt gattttttct attcttataa ataattttct
    16261 gcgacggctg tatctcctgg gtaggattct ttcttgccct ggatgatttg gtaacaatcg
    16321 gctcccttac ccgcaataat aactgcatct aattcgtgat ttgtgatagc cattgccgcc
    16381 ttgatggctt cttggcgatc cgcaatcttt tcaacaggat gattgatgta gctactaatt
    16441 tcatctgcaa tggccattgg gtcttcatag ttagggtcat cagcagtcag aaagacttga
    16501 atctcagggt gttgattgag gaggaggcca aagtccttac gacgactttc tcccttgttt
    16561 cctgttgatc ccagaaccag agcaatcttt ccggtttgat gagtttcaac cacattgatg
    16621 agttttttca gactatcccc attgtgggca tagtcgatga agaccttggc tccatttttc
    16681 tgagtgagga cttccatacg accaggaacg cgggttgcag cgatgccttt tttgatgtcc
    16741 tcaagacttg ctccgagacg gagacaagca agtccagcag caactgcatt ttcttggttg
    16801 aagttgccaa tgagttggat atcataatct ccagcgagtt tacccgtagc tgaaaagcta
    16861 aaggctttgg aattctcgat ttggttatca aattggctac catagaaatc atggtcttga
    16921 tcttcaacct gttctttcaa gactgagaag tggtccatgt cactgttaat gatgactgct
    16981 cggctatttt ccatcaagag acgcttgtgg tagaaatagt cttcaaagct agggtgttca
    17041 atcgggccga tatggtcagg agtgatgtta agaaagactc ctacatcaaa ggtcagtcca
    17101 tagactcgat ggactagata ggcttgactg gagacttcca tgatgaggtg ggtacggtca
    17161 ttttgcacag cctgattcat catgtcaaag aggtcaatac tctcaggggt tgtcaacgct
    17221 gacttaaaga aagtctcgcc atcaagagtt gtgttcatgg tcgacaacat agcaggtcta
    17281 tgcccttgag ataagatgtt ataggcgaaa taggttgctg ttgtcttacc cttagtacca
    17341 gtaaaggcaa ggagtttgag tttctcttgt ggattaccat agaactccat ggcaatcaaa
    17401 ctcatggctt tctttatatc gttcacaatg atgacaggga tatcgacttc gtagtccttt
    17461 tcagctacat accaagctaa accttgtgtt atagcagaaa gaaggtattc ttttttaaag
    17521 gcagcgcctt ttgcaaaaaa aagagtgtct tctgttactt ttcggctgtc gtagctgatg
    17581 ctatcaaaaa taactttgct gtagttgtag tggtaatgac cttggtcaat aatttcgcga
    17641 aaaaggccat ctttctttaa aatatctaat acggtttcaa tcttaatcat actttctatt
    17701 gtaaaccgaa agtcgtaaat ttacaagtaa caaggaaaag tttataatgg aagataagga
    17761 gtttttccta gttatcaaaa ttgaatgagg aatctatgtc gcacgaaaac aatcaccagc
    17821 aggcccagat gttacggggg actgcttggc taacggctag taactttatc agtcgcctac
    17881 tcggggctgt ttacattatc ccttggtaca tctggatggg ggcttatgca gctaaggcaa
    17941 atggtctctt taccatgggt tacaatatct atgcttggtt cttgttggtt tcaacagcgg
    18001 ggattccagt tgcggtggcc aagcaagttg ccaagtataa taccatgcga gaagaagagc
    18061 atagctttgc cctgattcgg agcttcttag gctttatgac aggactaggc ctggtttttg
    18121 ctttagtctt gtatgtcttt gctccttggc tagcagactt gtctggcgtg ggcaaagact
    18181 tgatcccaat catgcaaagc ttggcttggg gagtcttgat tttcccgtct atgagtgtta
    18241 tccgaggatt tttccaaggg atgaataacc tcaaacccta tgccatgagc caaattgctg
    18301 agcaggtcat tcgtgttatc tggatgctcc tagcaacctt tatcattatg aagctcggtt
    18361 caggagatta tctagcagcc gttacccaat caacctttgc tgcctttgtc ggtatggtag
    18421 ccagttttgc agtcttgatt tatttccttg cccaagaagg ttcactcaaa agagtctttg
    18481 aaacaggaga taagattaac agtaagcgtc tcttggttga taccattaag gaagccattc
    18541 cttttatcct gacagggtct gccatccagc tcttccagat tttggatcag ctgaccttta
    18601 tcaatagtat gagctggttt accaactaca gcaatgagga cttggttgtc atgttttctt
    18661 atttctcagc caatcctaat aaaatcacga tgattttgat ttctgtaggg gtttcgattg
    18721 ggagtgttgg tttgccactt ttgacggaaa actatgtcaa gggggacttg aaagcagctt
    18781 ctcgtctcgt tcaggacagt ctcaccctac tctttatgtt cttgctacca gcaacggttg
    18841 gagtggttat ggtaggagaa cctctttata cggtcttcta tggtaagcca gatagtttgg
    18901 ctctgggctt atttgtcttt gcagttttgc agtctattat tttaggcttg tacatggtct
    18961 tgtctccaat gcttcaggcc atgttccgca accgcaaggc cgttctctat tttatctatg
    19021 gttctattgc caagctagtc ttgcaactac ctaccatcgc cctcttccac agttatggtc
    19081 ctttgatttc aacaaccatt gctctcatca ttcctaacgt cttgatgtat cgggatattt
    19141 gtaaagtaac tggtgtcaag cgcaaggtga ttttgaagcg aaccatttta atcagtttgc
    19201 tgaccctagt catgtttctg ttaataggaa ccatccagtg gctgttagga tttttcttcc
    19261 aaccaagtgg acgtttgtgg agcttctttt atgtagctct tgtcggtgcc atggggggtg
    19321 gactttatat ggttatgagt ctgcgtacct atttattaga taaggtaata ggaaaagccc
    19381 aagcagatcg cctgcgagca aaatttaagc tttcgtaagg tagcatttat aaataaaagg
    19441 aataagttga acaatctttt aggagaactt gaagcttatt ccttttttag cgtaaaaata
    19501 gctaaaatta tatttcaaaa atagaaaatg aggctttttt cgattttttg gaaaaaatca
    19561 ttgactaaac aaactaaacg ttgtataata aaacaaatat ttaaagcagg aggttccgga
    19621 aatgaaaaag tctaagagca aatatctaac cttggcaggt cttgtcctgg gtacaggagt
    19681 tttattgagc gcgtgtggaa attctagcac ggcgtcaaaa acatacaact atgtttattc
    19741 aagtgatcca tctagcttga actatctagc agaaaaccgc gcagcaacat ccgatattgt
    19801 tgcaaatttg gtagacgggt tattagaaaa tgaccaatat gggaatatta ttccatcatt
    19861 agcagaggat tggactgttt ctcaggacgg tttgacctat acctacaaac ttcgtaagga
    19921 tgccaagtgg tttacttctg agggagaaga atatgcgcct gtaactgccc aggattttgt
    19981 gacaggtttg caatatgcag ctgataaaaa atcagaagcc ttgtatctag tgcaggactc
    20041 tgttgctggt ttggatgact atatcactgg taaaacaagc gacttttcaa ctgtcggtgt
    20101 caaggcactt gatgaccaaa cggttcaata tactttggtt aaaccagaac tttactggaa
    20161 ttcaaaaaca cttgcaacga tactttttcc tgttaatgca gatttcctga aatcaaaagg
    20221 ggatgatttt gggaaggcgg atccatctag tattttgtac aatggacctt tcttgatgaa
    20281 agcacttgtc tcaaaatctg ctattgaata taagaaaaac cctaattact gggatgctaa
    20341 gaatgtcttt gtagacgatg tgaaattgac ctactatgat ggtagcgacc aagaatcact
    20401 ggaacgtaat tttacagctg gtgcttatac tacggctcgt ctttttccta acagctccag
    20461 ctatgaaggg attaaagaaa aatacaaaaa caatatcatc tatagtatgc aaaattcaac
    20521 ttcatatttc tttaatttta acctagatag gaagtcttac aattatactt ctaaaacaag
    20581 tgacattgaa aagaaatcga ctcaggaagc agttctcaat aaaaacttcc gtcaggctat
    20641 caattttgct tttgacagaa catcttatgg ggctcagtct gaagggaaag aaggtgcaac
    20701 aaagattttg cgtaacctag tggttcctcc aaactttgtc agtatcaagg gaaaagactt
    20761 tggtgaagtt gtagcctcta agatggtcaa ctatggtaag gaatggcaag gtatcaactt
    20821 tgcggatggt caagaccctt actacaatcc tgagaaagcc aaggctaagt ttgcggaagc
    20881 taagaaagaa ctcgaagcaa agggtgttca attcccaatc cacttggata agactgtgga
    20941 agtaacagat aaagtaggca tacaaggagt tagttctatc aaacaatcaa ttgaatctgt
    21001 tttaggttct gataatgtag tgattgacat tcagcaatta acatcagatg agtttgacag
    21061 ttcaggctac tttgctcaaa cagctgctca gaaagactat gatttatatc atggcggttg
    21121 gggacctgat tatcaagacc cgtcaaccta tctcgatatt tttaatacta atagtggagg
    21181 atttctgcaa aatcttggac tagagcctgg tgaggccaat gacaaggcta aggcagttgg
    21241 actggatgtc tatactcaaa tgttggaaga agctaataaa gagcaagatc cagccaaacg
    21301 ttatgagaaa tatgctgata ttcaagcttg gttgattgat agttctttag ttcttccaag
    21361 tgtttcgcgt gggggaacac catcattgag aagaaccgta ccatttgcta ctgcctatgg
    21421 tttaaccggt acaaaagggg ttgaatcata taaatacctc aaagtacaag ataagattgt
    21481 cacaacagac gaatatgcaa aagccagaga aaaatggttg aaagaaaaag aagaatccaa
    21541 taaaaaagcc caagaagaat tggcaaaaca tgtcaaataa aatctaattg tagataagtt
    21601 tgtgtaagaa gtctttgtgc ttaaaaacgc ataatatcag gtatataaaa tcctagatgt
    21661 tatgcgtttt gttataggaa gatttattag attttatcct caaattgagt tattcccgac
    21721 tttatccgtt gaaatattat aaggagtatt tatgaaatta aaattattaa gagtagatac
    21781 taaggtgatt atggggagtt tcttacttgt tctgtctagt ctacttgctt tgttgcttcc
    21841 ccttatctta aagggtttaa tagatgggag ttctattgaa aatataggtt ccaaagtatt
    21901 tcaatcgttt ttgattttta ttggtcaagc cttgttttct tctattggtt actatctgtt
    21961 tagtcaatcg ggtgaaaaaa agatagcaaa aatcaggaaa aaagtgatag aggggttgat
    22021 ttatgcagag aaatccttct ttgataagag ccaaagtggg gagttgactt ctgccattgt
    22081 caatgacacg agtgtcattc gtgagttttt aattacgact ttcccaaata ttattctgag
    22141 tttagttatg gtacttggtt ccattgtagt cttatttagt cttgattgga atctttctct
    22201 acttttattc atcactcttc cttgtatgat gtttattatc ttgctccttt ccaatatcag
    22261 tgaaaagtat agtcgtcgtt tacaggagga aatcggattt ttaacagggc aattgactga
    22321 aaagattcaa gaacatgaat taattaagac taaccaagca gagaaaagtg tacaggatgt
    22381 tttggataat tgtattgaaa gggtacaaaa caattcactg aaatcagata gggttacttc
    22441 ttttgagacc ccgtttgctc tcttatttat ctttgcgact atagctgtga tgctaaccta
    22501 tgggggttat cgggtcagcg caggatatat atctgtggga accttggttt cgtttttgat
    22561 ttacctcttt caattactta atcctattag taatatagct aattttgtaa ctgtttattc
    22621 taggagcaag ggatcttcag ttgcactgga gaacttgctt gcagttccta aagaaaaatt
    22681 tgagtgagga aaatcggtat caggacaagg gttgaatttt aaccatgtct attttggtta
    22741 tgatgaaaat cgacctgtct taaaggatat tacttgttca attttcaagg ggcaaaaaat
    22801 tgcttttgtt ggaccatctg gatcaggaaa atcaacgatt gtgcgtttgt tagagcggtt
    22861 ttataaaccg ctttcaggag atattctaat ggagcaatca agtatatatg attttaactt
    22921 aaaagaatgg agaagtaaaa tcgcttagta actgatgatg aattgatgaa agtgctagac
    22981 ttagtatcac taggtgatga gattcgctcc atgaaagagg gactagatac tgaagttggt
    23041 gaacgcggac gactcttgtc aggggggcaa agccaaagac ttcaaatagc tagagcctac
    23101 ttaaaagatg ctgaaattct tatatttgat gaagccactg ctaatcttga tgcggattct
    23161 gagtatgcga ttatcagtag cctctattct gtattaaagg agaagacggt tgtgattata
    23221 gcgcatcgtt tgtcaacggt aaaagatgtg gattgtattt tcttcttaga ggaggggaaa
    23281 atcactggct caggaactca taaggaacta ctggaaaatc atgagcgtta tgctcgtttt
    23341 gtgcaggagc aaatgataga gtgaaggtgt cttttgagat tcaccatttt atagtctatt
    23401 aaagggagca ggaaaaactc cctttttata tagtttgaaa ctataactag ctcttgaaaa
    23461 gaagaaaatg agttgatgaa aataagtggt acaatagtta ctatagattt ggaggtattg
    23521 tatgagcaag gaattacaca ttaacacaat tttggcccag gcgggtatta agtcagatga
    23581 agcgacaggt gcattggtga caccgcttca tttttcaacg acctatcagc atccagagtt
    23641 tggtcgatct actgggtttg actatacgcg cactaaaaat ccaactcgta gtaaggctga
    23701 ggaagtcttg gcggctattg agtcagcaga ctatgcccta gcgactagct cagggatgtc
    23761 agctattgta ctggccttta gcgtctttcc agtaggaagt aaggtcttgg cagtgcgtga
    23821 tctttacggt ggttcttttc gctggtttaa ccaagtggag caggaaggtc gtttccattt
    23881 tacctatgcc aatacagaag aagagttgat tgccgagtta gaaaaggatg tggatgttct
    23941 ctatatcgaa accccaacca atcccttgat gttggaattt gatatcgaaa aactagcaaa
    24001 attggctcat gctaagggtg ccaaagtggt ggtggacaat accttctata gccctatcta
    24061 ccaacgtccg attgaagatg gagcagatat cgttctccat tcagcaacca agtatctagc
    24121 aggccacaat gatgtcttgg ctggagtggt tgtgaccaat agtttagaac tatacgagaa
    24181 gcttttttac aatctcaata caacaggggc agtcttgtct ccatttgaca gctaccagtt
    24241 gcttcgtggt ctcaagacct tatctcttcg tatggagcgc tcaacagcta acgcccaaga
    24301 agtggttgcc tttttgaagg attctccagc agttaaggaa gttctctaca ctggtcgtgg
    24361 aggcatgatt tcctttaaag tagccgatga aacacgcatt cctcatattt tgaacagtct
    24421 caaggtcttc tcttttgcgg aaagtttggg cggagtggaa agtcttatta cttatccaac
    24481 gactcaaact catgctgata ttccagcaga agtacgccat tcttatggtt tgacagatga
    24541 cctcttgcgt ttgtctattg ggattgagga tgctagagat ttgattgcag atttgcgcca
    24601 agccttagaa ggataagaca aagatgggaa aatatgattt tacaagcctg cccaaccgtt
    24661 tagggcacca tacctataaa tggaaagaaa cagaaacgga tagtgaagtt ctaccagctt
    24721 ggatagcgga tatggacttt gtggtcttgc ctgaaatccg ccaagccgtg caaacttacg
    24781 cagaccaact ggtttatggt tatacctatg ccagtgaaga cttaattaag gaaattcaaa
    24841 agtgggaagc tacacaatac ggttacaact ttgacaaaga ggctcttgtc tttatcgagg
    24901 gtgtggtacc agccatctca acagctattc aaacctttac aaaagaaggc gaggcggttt
    24961 taattaacac gcctgtctac ccaccctttg ctcgcagtgt caagttgaat aatcgtagat
    25021 tgattactaa ttccttagtg gaaaaggatg gtctgtttga gattgacttt gaccaacttg
    25081 aaaaggattt ggtggaagag gaggttaaac tctatattct ttgcaaccct cacaatcctg
    25141 gtggacgtgt ttgggaaaaa gaagtgttgg agaagattgg ccaactttgc caaaaacacg
    25201 gtgttttgtt agtttcggat gagattcacc aagatttgac cctctttggt cacaaacacc
    25261 agtctttcaa taccatcaat cctgccttca aaaattttgc tatcgtcttg agcagtgcca
    25321 ctaaaacatt taatattgct ggaacaaaaa attcctatgc agtcattgaa aatcctaagt
    25381 tgagactggc tttccagaaa cgcctgttgg ccaataatca gcatgaaatt tcaggcttgg
    25441 gttatttggc gacagaagct gcctatcgat acggtaaaga ttggctagag gaactcaagc
    25501 aagtctttga agaccacatc aattatgtgg tggatctatt tggaaaagag actaaaatca
    25561 aggtcatgaa accgcaaggt acctacttga tttggcttga cttttcagcc tatgacctga
    25621 ctgatgaaac attgcaagag ttgttgagaa atgaagccaa ggttatcctc aaccgtggtt
    25681 tggattttgg agaggaagga agtctccatg cccgcatcaa tatagctatg cccaaatctc
    25741 tgttgcaaga agtctgtcag cggattgtgg ctacttttgc caaacgttaa aaatccagcc
    25801 ttctaggaga aaagtcttcc tagaaggcta ttttcatagg cgaaaatatg gtataataaa
    25861 aagataaggt aaaggtgaaa atatggctaa attgattccg gggaaaattc gtatcgaagg
    25921 tgttgccctt tatgaaactg gtaaggttga tatcatcaag gaaaagaaca atcggctcta
    25981 cgctcgcgtt gcagaagaag aactgcgcta tagtttagag gatgatttgg ttttttgtgc
    26041 ctgtgattct tttcaaaaga ggggctactg tgtgcatttg gcagcgctag agcattttct
    26101 gaaaaatgat gagcgtggtc aggaaatctt gtggagtctg gaagaaggtc atgaagaaaa
    26161 agaggccgtt gaaaccaagg tgaccttggg tggcaagttt ttgaatcgaa ttttatctcc
    26221 aaaatcagaa tgcgcctatg agttatcagc tgtgggtcag gtggaagcag gaaccaatca
    26281 tatcttgtgg actctgagaa tcggccaaat caatagccaa aaatactatg tcattcggga
    26341 tattccactt tttttaagga ttgtcgagca gagaaagtcg tatatgattg gcaagattta
    26401 tgaggagtct ctttcttggg aagcctttga tgatgccagc caagaacttt tgactttctt
    26461 gcacggacta acggaggaag ggctggttcc agacctcttt ttccaaaacc aaggtcggca
    26521 cctctttttc cctctgacct tttttgagca gggtgtgaat ttactgatgg ccttgcccca
    26581 ttttcaattt gaccatcaag tggatagcta ccaaaccctg ctttttcagg atatgcatgc
    26641 tgatgccaat ctatttgcct ttacagtaac agaatactcg gactattttg aaatggaaat
    26701 cagtgagagt cctagggtca atgtctttta tcagggagca gtacttttcc ataaaggtca
    26761 ggtttatttt ttaacagacc agcagatgcg tcttctcaag gaaattaaag cgttacctgt
    26821 gggtcaacaa ggaaagaaat acctgcaatt tgatagcagt gatcgagaca aattggcctc
    26881 ctgtctgact ctttttatcc agatgggcac cgtttcagct ccagaacgtc tacaaatcaa
    26941 aacttttgcg ccctcttttt actttgacag ggaagaagat aatcggattc gtttagaaat
    27001 tcagtttgat tatggaaata gacaggtatc tagccgacaa gagctagaag aattaccatt
    27061 ttcaagtgac gcggacttag aagaaagaat tttccaagtc tgtttggctg cgggctttga
    27121 agccgatttt caatcttggc gtcaggcctt aaaagcggag tctgtctatc atttttttca
    27181 tgatatcatt ccagtctttg aaaaactcgg gcatgttgac ctatcagata aactagaaga
    27241 gctttatagc ctagcgagtc ctcaggtgca gattgcctcc aagggaggtc tcttggaaat
    27301 ccagtttgat tttcaaaata ttgcccagga ggaaatcgac caagccatgc aggccttggt
    27361 tgccaatcag gatttttata ttgattcgtc taatcaagtt tactttttcg atgaagaaac
    27421 caagaaaatt cgccaaaatc tacaggaact ggggcaattt gaattaaaag atgggacctt
    27481 gcaggctcgg aaatccttgg cctacagttt agctcatctc tttgaagggc gagaccgtgt
    27541 ttctttttca caagaattcc agaatctggc ccaggatttg acgcatccag aggactttcc
    27601 tttgcaagca actcaggtca aggctgactt gcgagattat caggaaaagg gaattggctg
    27661 gttgcagatg ctccatcatt atggttttgg tgggattttg gctgatgata tgggacttgg
    27721 gaaaaccctt cagaccattg cttttttgac tagtcaagtg acaaaagaaa gtcgagtctt
    27781 gattctcgct ccgtcgggtt tgatttacaa ctgggcggat gagtttcaga aatttgcccc
    27841 acagttggat gtggctgttg ttcatggttt gaaagcaagt cgtgaggaga ttcttgccga
    27901 gagccatcaa atctatgtga ctagctatgc caccttccgt caggacagtg agttttatca
    27961 ggggatggcc tttgacttcc ttttcttaga tgaggctcag gtcatgaaaa atgcccagac
    28021 caagattgcc cagaccttga gacaatttgt ggtgccgtcg gtctttgcct tgtcaggaac
    28081 tccgattgaa aaccatctgg gtgagttgtg gtctattttc caaatcgtca tgcccggact
    28141 tttgccaagc aagaaagaat ttatgaaatt gccagcagag cgagtggctc agtttatcaa
    28201 gcctttcgtg atgcgacgca agaaagaaga agttctgact gaattgccag acttgattga
    28261 ggtggtttat aaaaatgaac tggaagacca gcaaaaggct atttaccttg cccagttaca
    28321 acagatgcga gaccatctgg ctcaagtgtc agaacaggaa tttcagcgaa gtcgtgtgga
    28381 aattttatct ggtttgatgc gcttgcgtca aatatgtgac actcctgccc tgtttatgga
    28441 agattatcag ggagccagcg gcaaactaga tagtctccga gacctactgg tacaggtggc
    28501 agacggcgga caccgtgtct tgattttctc tcagttcaag ggaatgttgg aaaaaattga
    28561 acaagaactg ccagacttgg gcctgacttc ctttaaaatt acgggttcaa ccccagccaa
    28621 ggaaagacaa gacatgacca aggcctttaa tcaaggagaa agagatgcct ttctgatttc
    28681 cctcaaggct ggtggggtcg gtctgaacct gacaggtgca gatacagtga tcttggttga
    28741 cctttggtgg aatcctgcgg tggaagcgca agccattggc cgtgcccatc ggatgggtca
    28801 ggaagaaacg gttgaggtct atcgcttggt gaccaaggga accattgaag aaaaaattca
    28861 ggaactccaa gaacaaaaga aacatctggt gtcacaagta ttggacggca cagagtcacg
    28921 tggtagtctg accctagcag aaattagaga aattttggga atttctgaag ccagcacttg
    28981 aaaaatcaag acaatacgct ataatgaagt gttgaaagga aatagaaaat gaaacaagaa
    29041 cgatttccat tggtgtcaga tgacgaggtc atgttgactg aaatgccagt catgaatctc
    29101 tatgatgagt ctgatctgat cagtaatatc aagggtgagt atcgagataa aaattattta
    29161 gaatgggctc ctattgctga agaaaaacca gtaaaaccga ttgaaaagca agtagggaaa
    29221 cctaaaaaag ctcctttagg ggttaaaaaa gaaggaaaga gctatgcgga ggtggcgcgt
    29281 gaagaagcgc gtgcagactt gaaaaagaaa cgctctgcta actatctaac tcaggatttc
    29341 agccttgcga gacgtcattc tcagcccagt ctagttagac agggcaatca accgacaact
    29401 cctttccaaa aggaaaatcc tggtgaattt gtcaaatata gccaaaaatt gacccagtct
    29461 cattatatct tggcggaaga agttcattct atccctacca agaatgaaga agtgtcagca
    29521 cctgctccaa agaaaaacaa ttatgatttt ctaaagaaga gccaaatcta caataaaaaa
    29581 agtaaacaaa cagaacaaga acgtcgggtt gcccaagagt tgaatctgac cagaatgaca
    29641 gaatagggag aaaacatgtc aaagacatat cattttatcg gaattaaggg atcagggatg
    29701 agtgccttgg ccttgatgtt gcaccagatg gggcacaagg ttcagggatc agatgttgaa
    29761 aagtactact ttacccaacg cggtcttgag caggcaggaa ttaccattct tccttttgat
    29821 gaaaagaatc tagacggtga tatggaaatt atcgctggaa atgcctttcg tccagataac
    29881 aacgtcgaaa ttgcctatgc ggaccaaaat ggtatcagct acaaacgtta ccatgagttt
    29941 ctaggtagct ttatgcgtga ctttgttagc atgggagtag caggagcaca tggaaaaact
    30001 tcaacgacag gtatgttgtc tcatgttttg tctcacatta cagataccag cttcttgatt
    30061 ggagatggga caggtcgtgg ttcggccaat gccaaatatt ttgtctttga atctgacgaa
    30121 tatgagcgtc acttcatgcc ttaccaccca gaatactcta ttatcaccaa cattgacttt
    30181 gaccatccag attatttcac aagtctcgag gatgttttca atgcctttaa cgactatgcc
    30241 aaacaaatca ccaagggtct ttttgtctat ggtgaagatg ctgaattgcg taagattacg
    30301 tctgatgcac caatttatta ttatggtttt gaagctgaag gcaatgactt tgtagctagt
    30361 gatcttcttc gttcaacaac tggttcaacc ttcaccgttc atttccgtgg acaaaacttg
    30421 gggcaattcc acattccaac ctttggtcgt cacaatatca tgaatgcgac agccgttatt
    30481 ggtcttcttt acacagcagg atttgatttg aacttggtgc gtgagcactt gaaaacattt
    30541 tccggtgtta aacgtcgttt cactgagaaa attgtcaatg atacagtgat tatcgatgac
    30601 tttgcccatc atccaacaga aattattgcg accttggatg cggctcgtca gaaataccca
    30661 agcaaggaaa ttgtagcagt ctttcaaccg cataccttta caagaaccat tgccttgttg
    30721 gacgactttg cccatgcttt aaaccaagca gatgctgttt atctagcgca aatttatggc
    30781 tcggctcgtg aagtagatca tggtgacgtt aaggtagaag acctagccaa caaaatcaac
    30841 aaaaaacacc aagtgattac tgttgaaaat gtttctccgc tcctagacca tgacaatgct
    30901 gtttacgtct ttatgggagc aggagacatc caaacctatg aatactcatt tgagcgtctc
    30961 ttgtctaact tgacaagcaa tgttcaatag gatgttccca tggaaattcc aattaagatc
    31021 attcaggcaa gcaagtctga tttgcctgag ataggggcac ttcagacctc gtcttttcca
    31081 gctgaaaagc agcaactttc ccatatttta gaagaaagta tccgtaagtg tgcggatacc
    31141 tttcttctag ctagggatga aaatcaactt ttaggctata ttttatcaag tccccagtca
    31201 gacaatccgc aatatctaaa agtacattct ttagtcatcg agtctgacca tcagagacag
    31261 ggcttgggaa cacttcttct tgcagccttg aaagaggtgg cagttgatct ggattacaaa
    31321 gggattcgtt tggagagtcc tgatgagctg ctttcctatt ttgaaatgaa cggttttgtt
    31381 gatgaagaag caactttgct ctatgcaact agccagggct atagtatgat ttggtttaat
    31441 cccttttatc tggaggaaca atgaaaatca gacaagcaag attagaagat ttggatcgga
    31501 ttgttgaact tgaatttgaa aatttttcgg tcgaagaagc cattcctcct tctgtctttg
    31561 aagcacattt gagagaaatt cagacctctt ttctggttgc tgaaaaagaa ggaagaatca
    31621 tgggttatat cgaaggacca gttggcctgc accgccatct gcaagaccag tcttttacag
    31681 aagaaataaa agactatagt catgagcctg gtggttatat ctctgtgacc tgtctgtcta
    31741 ttgccaagga ggcacaggga ctcggactgg gtcagaaatt gctgacagct ttgaaagaag
    31801 ttgctcttga agatgagaga gatggcatta atctaacctg tcatgactat ctcatcgcct
    31861 attatgaaaa acatggattt gtcaacgaag gccagtccca gtcaaccttt gcaggggaaa
    31921 catggtatga tatggtctgg gaaatgaaaa aataagttag gaaaagtatc atagattgct
    31981 ttttttggac ttttaagata taatattatg gattatcaac aaggaggaaa aacttttgag
    32041 tgaaaagtca agagaagaag agaaattaag ctttaaagag cagattctga gagatttaga
    32101 aaaagtaaaa ggctatgatg aagttctgaa agaagatgag gcagtagttc gcactcctgc
    32161 aaatgaacct tcagctgaag aactcatgac tgattccttg tcaacggtag aggagattat
    32221 gagaaaagct cctaccgtgc ctactcaccc aagtcaaggt gtaccagctt ctccagcaga
    32281 tgagattcaa agagaaactc ctggtgttcc aagtcatcca agtcaagatg taccttcttc
    32341 tccagcggaa gaaagtggat caagaccagg tcctgttaga cctaagaaac ttgaaagaga
    32401 atacaatgaa accccaacaa gggtagctgt ttcctatacg acggcagaga aaaaagcaga
    32461 acaagcaggt ccagaaacac ctacgcctgc tacagaaaca gtggatatca tcagcgatac
    32521 atcacgtcgt agccgtagag aaggagcaaa acccgttaat cctaagaaag agaagaagtc
    32581 acatgtgaaa gcttttgtga tttcattcct tgtattcctt gccttgctct cagcaggtgg
    32641 ttactttggt taccagtacg tgctagattc tttgttacct atcgatgcta attctaagaa
    32701 atatgtgacg gttggaattc cagaaggttc aaacgttcaa gaaatcggta cgacgcttga
    32761 aaaagctggt ttggtaaagc atggtctgat ttttagtttt tatgccaagt ataaaaatta
    32821 taccgacttg aaagcaggtt actacaattt gcaaaagagt atgagtacag aagacttact
    32881 caaagagttg caaaaaggtg gaacagatga accgcaagaa cctgtacttg cgactttgac
    32941 aattccagaa ggttatacct tggatcagat tgctcaagct gtgggtcaat tgcaaggtga
    33001 cttcaaagag tctttgacag cggaggcttt cttggctaaa gttcaagatg agacgtttat
    33061 cagtcaagca gtagcgaaat atcctacttt actggaaagt ttgcctgtaa aagacagcgg
    33121 tgcgcgttat cgtttggaag gatacctttt cccagctaca tactctatca aggaaagcac
    33181 aactattgag agcttgattg atgagatgtt agctgctatg gataagaacc tatctcctta
    33241 ctatagtact atcaaatcta aaaatttgac tgtcaatgag ttgttgacca ttgcttcctt
    33301 ggtcgaaaaa gaaggtgcca agacagaaga tcgtaagctc attgcaggtg tattctacaa
    33361 tcgtttaaat cgtgatatgc cacttcaaag taatattgca atcttgtatg cccaaggaaa
    33421 actggggcaa aatatcagtc tagctgagga tgttgcgatt gataccaaca ttgattcacc
    33481 ttataatgtt tataaaaatg taggtctcat gcctggtcca gtcgatagtc caagtctgga
    33541 tgcgattgag tcaagcatca atcaaactaa gagcgataac ctctactttg tagcagatgt
    33601 cacagaaggc aaggtctact atgctaacaa tcaagaagac cacgaccgca atgtcgctga
    33661 acatgtcaac agcaaattaa actaaacaaa ctaaaattat gtgatacgtc acataatttt
    33721 ctttagaaaa tattatcaga agaaagttga gaaaaatggc agaaaaaaca tatcctatga
    33781 cccttgagga aaaggaaaaa cttgaaaaag aattagaaga attgaaattg gttcgtcgac
    33841 cagaagtggt agaacgcatt aagattgccc gttcatacgg tgacctttca gaaaacagtg
    33901 agtacgaagc agctaaggat gaacaagcct ttgtcgaagg acaaatctct agcttagaaa
    33961 caaaaatccg ctatgctgaa atcgtcaata gcgacgcagt tgcccaggac gaagtagcga
    34021 ttggtaaaac agtcaccatc caagaaattg gtgaggacga agaagaagtt tatattatcg
    34081 taggttcagc tggtgcggat gcctttgcag gtaaggtttc aaatgaaagc ccaattgggc
    34141 aggccttgat tggcaagaaa acaggtgaca cagcaaccat tgaaacgcct gttggtagct
    34201 atgatgtaaa aatcttgaag gttgaaaaaa cagcctaaaa acagaaaaag gagtggggag
    34261 gcgatgtgct tcactcactc ctttttccat tttgctattc ttcgaaaatc tcttcaaacc
    34321 acgtcagcgt cgccttgccg tatgtatggt tactgacttt gtcagtttca tctacaacct
    34381 caaaac
//
LOCUS       DAVBCR010000017        32793 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCR010000017 DAVBCR010000000
VERSION     DAVBCR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.017"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            218..448
                     /locus_tag="VQ508_000728"
     CDS             218..448
                     /locus_tag="VQ508_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2489761.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            852..1565
                     /locus_tag="VQ508_000729"
     CDS             852..1565
                     /locus_tag="VQ508_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2489762.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            1623..2225
                     /locus_tag="VQ508_000730"
     CDS             1623..2225
                     /locus_tag="VQ508_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489763.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2361..3014
                     /locus_tag="VQ508_000731"
     CDS             2361..3014
                     /locus_tag="VQ508_000731"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2489764.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3024..3488
                     /locus_tag="VQ508_000732"
     CDS             3024..3488
                     /locus_tag="VQ508_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2489765.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            3501..4769
                     /locus_tag="VQ508_000733"
     CDS             3501..4769
                     /locus_tag="VQ508_000733"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2489766.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            4783..5043
                     /locus_tag="VQ508_000734"
     CDS             4783..5043
                     /locus_tag="VQ508_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489767.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ508_000735"
     CDS             5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ508_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2489768.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            6569..7537
                     /locus_tag="VQ508_000736"
     CDS             6569..7537
                     /locus_tag="VQ508_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2489769.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            7553..8545
                     /locus_tag="VQ508_000737"
     CDS             7553..8545
                     /locus_tag="VQ508_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2489770.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            8763..9806
                     /locus_tag="VQ508_000738"
     CDS             8763..9806
                     /locus_tag="VQ508_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2489771.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ508_000739"
     CDS             9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ508_000739"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2489772.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            11619..12608
                     /locus_tag="VQ508_000740"
     CDS             11619..12608
                     /locus_tag="VQ508_000740"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2489773.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ508_000741"
     CDS             complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ508_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2489774.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14329..15879)
                     /locus_tag="VQ508_000742"
     CDS             complement(14329..15879)
                     /locus_tag="VQ508_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2489775.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(15895..16674)
                     /locus_tag="VQ508_000743"
     CDS             complement(15895..16674)
                     /locus_tag="VQ508_000743"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2489776.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ508_000744"
     CDS             complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ508_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2489777.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(17719..17895)
                     /locus_tag="VQ508_000745"
     CDS             complement(17719..17895)
                     /locus_tag="VQ508_000745"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2489778.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ508_000746"
     CDS             complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ508_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2489779.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24047..25225)
                     /locus_tag="VQ508_000747"
     CDS             complement(24047..25225)
                     /locus_tag="VQ508_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489780.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25395..29045)
                     /gene="addA"
                     /locus_tag="VQ508_000748"
     CDS             complement(25395..29045)
                     /gene="addA"
                     /locus_tag="VQ508_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2489781.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29042..32317)
                     /gene="rexB"
                     /locus_tag="VQ508_000749"
     CDS             complement(29042..32317)
                     /gene="rexB"
                     /locus_tag="VQ508_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2489782.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(32517..32672)
                     /locus_tag="VQ508_000750"
                     /pseudo
     CDS             complement(32517..32672)
                     /locus_tag="VQ508_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9298 a         6449 c         6757 g        10289 t
ORIGIN      
        1 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
       61 agttaaggag taatgatgaa aaaaagaaca atactattat tgatggccag tctgttagct
      121 cttgtcttag gagcatgtgg tttcttggac atattgatcc tggatcattc tcatcaggat
      181 tactctttac tgctatttta gaaactgggg tggtttgatg gaaagtattg gtattggtct
      241 tgttatcgtt tcacattcca aacacattgc agaaggtgtt gttgaactga ttagtaaagt
      301 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      361 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      421 tgacctaggt tctgctataa aatgttaagt aaatgatttc aaaatcagaa agggattgct
      481 ttgggcagtt cctttttaat gtaaatgtaa aaggagtaga atcatagtgt ttctaaattg
      541 tgaatcaatc aaaactgatt gtgatggggc tattctagct ttagaatcct tcaaaaatta
      601 aaatttaagg caatcaatta cttggaagag tatgaaagca tttagtttat aggaattcta
      661 ggcctagaat tacatatgta tttatgaaga taggggtgtt cgatagttag tattgttctg
      721 ttctgaaaga tttgagctgt cagttgtata gaaagtgttc gaattttttt taagtgatta
      781 aattagttaa ttgtatgagg ttctttatga tataatgttc ttaatgaatt ttcagaaagg
      841 aaaacctcaa attgttctac aaatttctac tcttcgacct cgaccacact cttcttgatt
      901 ttgatgctgc tgaggatgtg gctttgaccc aacttctaaa agaagaagga gttgcggata
      961 ttcaggctta taaagattat tacgttccta tgaacaaggc tctctggaaa gacttggagc
     1021 tgaagaaaat cagtaaacaa gagctggtta acacgcgctt ttctcgttta tttgctcatt
     1081 ttggacagga aaaagacggt agttttcttg cccagcgtta ccaattttac ctcgcccagc
     1141 agggacaaac actatcgggc gctcatgatc tcttggacag cctcattgag cgtgattata
     1201 acttgtatgc tgcgacaaat ggcattactg ccattcagac aggacgtttg gctcaatctg
     1261 gtctagcacc ttatttcaat caagtcttta tctcagaaca gttgcaaact caaaagccgg
     1321 atgctctctt ttatgaaaag attggccagc aaattgctgg atttagtaaa gaaaagacgc
     1381 tgatgattgg agattctcta accgccgaca ttcaaggtgg caataatgcg gggattgata
     1441 ctatctggta taatcctcat cacctcgaaa atcacacaca agcccagccg acttacgaag
     1501 tctattctta ccaagacttg ctggattgtt tagataaaaa tattcttgaa aagatcacat
     1561 tttaaaggag acgagctaat gactacaaaa aagctaatat tactattgaa gagtacattg
     1621 aaatgtctga agttgatttt aatgaagctg ttaattatga atttacatct gacacttgtc
     1681 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     1741 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     1801 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     1861 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     1921 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     1981 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2041 ttcgattttt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
     2101 aagtttctaa agaaactcct ttttctgaaa tggattttaa tatgtttaaa aatatttcag
     2161 aaaagataat cttcgaaaga ggtagtaaaa tgcttaatga tttaaaaagt aattataaaa
     2221 aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatagtc gatttattaa
     2281 atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag
     2341 taaaaatgaa ggagtcggct atggaacact cgtcttggca tgctttgatt aaggcgcaat
     2401 tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga
     2461 ttatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag
     2521 ttaaggtggt aattctaggg caagacccct atcacggacc aggtcaagcg cagggcttga
     2581 gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat
     2641 tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct gagcaaggag
     2701 tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc
     2761 aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag
     2821 tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggccttagtt accaatcctc
     2881 atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg
     2941 gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg
     3001 attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg
     3061 ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat
     3121 ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggcgcgtg
     3181 aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc
     3241 ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg
     3301 gcgacttgat tgactgcaat gaggggacgc tagaatgggt tccctatgac gaggttttga
     3361 gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct
     3421 tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct
     3481 atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcctaagt
     3541 ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc
     3601 ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcttgtg gttgctcctg
     3661 gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc
     3721 atactggtgc cctggcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata
     3781 ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag
     3841 agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga
     3901 ctgactttaa ggcgctctta gaagctggtg cggttggttt ctctgatgac ggtattccgc
     3961 ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta
     4021 tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg
     4081 ctagagaaca tttccatatc tgcggtgcta ctggggtggc tgagtacgct atgatggcgc
     4141 gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg
     4201 aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag
     4261 tagcgccaca gcatttctct aagacagaag cacttctttt aacacaaggt agcaatgcta
     4321 agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat
     4381 caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat gaaaaaaatg
     4441 ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctcg
     4501 gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga
     4561 catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag
     4621 cagatatcac tatttttgat gccaaggctg accgccttgt ggactcccat tttgcttcca
     4681 aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct
     4741 gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa
     4801 cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc
     4861 aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt
     4921 ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat
     4981 aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa
     5041 tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa
     5101 aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt ttgttgatac
     5161 tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat
     5221 ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg
     5281 tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct gatttttacc aatttattta
     5341 tttggccttg ggcctatctg tggtcttgct ggggatggta cttttcttgg caccaacaat
     5401 cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt
     5461 tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc
     5521 gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg
     5581 atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc
     5641 tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt
     5701 taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta atatggataa
     5761 aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc
     5821 tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca
     5881 ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc
     5941 tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta
     6001 tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa ccttccttta
     6061 catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac
     6121 agcgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg cggcgccgct
     6181 tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg
     6241 ttattggggc gtagcaatcc ctgtggctac gctatttgat tccctaacag attttggagc
     6301 ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg
     6361 gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt
     6421 ttgtgaaaaa atattcttga taagaaaatc ccttgttttc acggggtttt cttttttatt
     6481 ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaaacggta aaatagtaag
     6541 gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga
     6601 aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa
     6661 gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg
     6721 ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca
     6781 agctattgct aaagggatcg acctcaacgg aatgatggct gaaatccttg gtaaatacac
     6841 tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct
     6901 tggtgccaat ggtatcgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca
     6961 gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga
     7021 aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta
     7081 ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca
     7141 tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag acggtaacaa
     7201 tgtcatcgat gtctatgaag gatttcagaa agctgtagac catgttcgca gtggcaatgg
     7261 tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg
     7321 taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct
     7381 ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc
     7441 gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc
     7501 gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac
     7561 aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga
     7621 tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc
     7681 tgttggaatg cttgaagaat ttggtccaga acgcgtccgt gactgtccga tttctgaagc
     7741 tgccatctct ggagcagcag caggagcagc catgacagga cttcgtccaa tcgtcgatat
     7801 gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac
     7861 acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa
     7921 cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc
     7981 aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc
     8041 tatccgtgat aaaaacccag ttattatact tgagtacaag tcagaattta accaaaaagg
     8101 ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca
     8161 aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc
     8221 tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta cccttgttcc
     8281 gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggttg ttctggtcaa
     8341 cgatgcccac aaaacaagtg gctatatcag tgaaatttca gctattattt cagaatcaga
     8401 agcatttgac tatctagatg caccaatccg ccgttgtgca ggagaagatg tgccgatgcc
     8461 ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat
     8521 ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag
     8581 ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata tcttatattc
     8641 gagcacgggc taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt
     8701 cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg
     8761 tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag
     8821 ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg
     8881 tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc
     8941 tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga
     9001 agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg
     9061 ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc
     9121 gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga
     9181 ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta
     9241 agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc
     9301 tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct tcattgacag
     9361 aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg
     9421 ataatggatt gatgacacct gttgtttaca atgctgagaa gatgagtctt tcagaactag
     9481 ttgtagcctt taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt
     9541 tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggcc
     9601 cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag
     9661 ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc
     9721 accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg
     9781 aaaccccaat ctcaatgttg atttaagagc aagagtattc atttttaaga tatagataaa
     9841 ggaaggaaga catggcctta gaaataatta tgccaaaagc cggcgtggat atgacagaag
     9901 gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt
     9961 tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga
    10021 ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaaattatc ggttaccttg
    10081 gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct agccctgtac
    10141 ctgtagcaag tacctcaaac gatgatggta agagcgatga tgcttttgat atcgttgtga
    10201 ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaactc ggcggtaagg
    10261 ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa
    10321 ccaagaccta ccttcataac gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg
    10381 gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat
    10441 ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagtta
    10501 ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta aatggttctg
    10561 aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg
    10621 tccctggtat gcaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag
    10681 tgccagaaag ccttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct
    10741 tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggaccgtata gttccagcta
    10801 tggatgcgga agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct
    10861 tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg
    10921 aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc
    10981 ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaacgaat
    11041 acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt
    11101 tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc
    11161 atgcagttgc caaattgaat ttgactcctg cagccatcta cactctccct gaagtagcag
    11221 cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta
    11281 actttgctgc taacggtcgt gctattgcat ctgacgcagc tcaaggtttc gtaaaagtta
    11341 tcgctgataa gaaatacgga gaaatcattg gtgtacacat tattggtcct gcagccgcag
    11401 aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga
    11461 agaccatcca cggacaccca acttactctg aagtgatgta tgaagcgttt gcagatgttc
    11521 taggaatggc catccattca cctaagaaaa aataaaacga agatagatta gactggaagg
    11581 ttatttccag tctctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt
    11641 caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg
    11701 aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga
    11761 acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag gtagttcgcc
    11821 gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct
    11881 ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata
    11941 ccctggctca actcggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg
    12001 gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt
    12061 gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata
    12121 aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat
    12181 taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag
    12241 agtacccaga gatgactgaa tacgtctttt cagaagaaga attggccgaa atcaatcaca
    12301 tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg
    12361 tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat
    12421 caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag
    12481 tagaagatgt ccttcgtggg gtgaaatacg aacgcgaaga tgttcttaag gcgctaaaaa
    12541 ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag
    12601 ttggataaat caaaaaaaga agttggccaa cttctttttt tgatatttat ttacataatt
    12661 taaaatacgt gaaatcataa actatctaga gcattctttt gttcatcatt aacaatatgg
    12721 gtatagaggt cagtgacttg tgtgctggca tgtcctagtt ggtgactgac taaaacttgt
    12781 gatttagtcg catcatagag cctagttgct agcgtatggc gcagtttatg gggtgttaca
    12841 cgtactttaa aatcctctga gtatttagca accattttct caacgctaga agcatcgata
    12901 cgattaggaa cacctctgta gagagttaaa aaaagggctg tatctgtttt ttccgtttta
    12961 tagcgttgat tccgaatggc cagataattc tctaaataag gtttagcaaa agcagcgaca
    13021 ttgactgagt cacgtttgcc accttttcga gtaacatcaa taaccatcat ttttagattg
    13081 agatctctta gatctagatt aacagcttca gataagcgaa caccagatgc caagagaagg
    13141 gcaataatgg ctaaatctcg ttctttattt ttgttgaatg atgagagagc tcgatttgaa
    13201 agctgttgtg ggtgctcttg atcgatataa gttagaaaac cttctgtttc atcacctaga
    13261 aagagttttt gcttgatatt ttcagctctg gcagcaaggg tttctttctt tttcttggtt
    13321 gaaacttttt tcattacatt acgatagaaa taaggttccc cctgatcgtt ttcaacctcc
    13381 tcggttagat acttgtaaag actagaaagt gctgataagg ttcgattgat agttgtctgt
    13441 gaaacacctt gttttgttgt attagcattc agcaagggac gttcacgtag ataaaggata
    13501 aaggattcca tgtctttctt agacatattt tccaaaactg ataaaggaat atcggatatt
    13561 ttatcagcgt ttgaaatacc agactccaaa acccagctga aaaatcggtc atattcctta
    13621 aggtattcgt acagggttgt aaaactgtag ggcacagcca gcttagattg gtagtattcc
    13681 agaacatacc agggcatgag ttgttttagt ttgtcgattc gttccagtaa aatctcacgt
    13741 ttcatgtatt ttctccataa gtaagtatac tagtagtata gcatagactt gtatattttt
    13801 caagaaaatt atagtttttc agaaagatgt tataggagtt tttcaaagtg ataaaaagac
    13861 ttagacctac aaacaaaaca gaataaggaa aataataaaa atctcaccca ctaaacttct
    13921 gttaatacta cataactgaa gagctatata aggagctatg agatacaaaa cacattgtat
    13981 aatagattga aactagaata gtacatctgg acttctaaaa cattgctaga aattgatttg
    14041 attgttctga ttgttttatc ctatttctat ttcatttcac tatataattg ctattgtttt
    14101 ctttttcatg attttaatct tactgtacag atttgataca gtaaactcct ttctagcttt
    14161 atactttatc atttctaact taattatagt cttattttta ataaagttca attatttata
    14221 tgtaaaattc ctcatcagta aaaaataacc gatctcattt ctgagaatcg gttttctaaa
    14281 taaatcaagc tgagtattta cataacataa attatgtaaa tactcatact acaacaacaa
    14341 atctttgact tttccaattt cactttttgg aataaccagg tgaatcatat cacccagata
    14401 cattctggtt gagccgttaa ctgtttggct cttgccatta tggacttgag ttgtgatgag
    14461 gacgttgtgt ggtaagttga gttcatgaac ttgtttccca gcaattttat cagaaactgg
    14521 tatttcgata agtgtaactt ctccttcgct agatacttct tctggaagca ttttttccag
    14581 catggcttca tagactggcg tacctttgag caaatccatg ataatataag aaacaagagt
    14641 gacaagacca agtggcatga ggttgcgaat atctcctacc atctcagtta cgaggatcat
    14701 agcggttaag ggagcttttg atatggctcc aaaatagcca ctcattccta gaatgacaaa
    14761 tatagggaat tgctcttgac tgacaagtcc aagattgaca cagataacac caactaagga
    14821 accaagcaaa gaaccaagag ctaaaatggg gaggaaaatt cctcctggca gtccacttcc
    14881 atagctaatc atactccaaa taaagcggat caaaaagtaa gctaataaaa cttggaaact
    14941 aaaattttgt tcagttaaag aaaggacaag ctgatttccg ccaccaatga tttgaggtaa
    15001 gaagattccg actggtatga taatgataaa agccaagatg ggataataag ccctatccaa
    15061 atggattttt tgaccaatca agtcataaac tcttccaacg tttaatacag ctttctcata
    15121 gagaaaacct gaaaatccaa ggaaaattcc catgacgaga tatatccaat actggtctag
    15181 ggtcatggga ggaatgttat ctggcatatc caatactggt gtcaaaccga acatgagtag
    15241 agacacaaag tttgctacga tgctggctgc tagagttgag acccagaaaa agcgcgaaaa
    15301 atggtgatag acttcttcta caacaaagag aagtgctgca ataggagcat taaaggctgc
    15361 ggctaaacct gctgcagctc cactggcaat caaggaacgt tcctctactg gactggattt
    15421 gagccacttg gcaattcctt taccaccaac tgctccaagt tgaatgctgg gtccctctcg
    15481 acccagcatg agtccactgg caatagcaag aatacctagc acatattttt tccaaagaat
    15541 gccccaccag ttgagggaca tgaggccttt cagttcggct tcgacttgag gaattcctga
    15601 gcctttaata tctttttctg accgtgttag tttggcactg agccaacaga tgagtatata
    15661 aaacaaaacc agtacaaaaa gattgcgcac taagtaccct tgatcttgat aaactccttg
    15721 tatcaggtgg aagccctttt caattaagaa acgaaaggat ccgacgataa ttccaacgat
    15781 gaggccgaca atgattcctc gaccaacttg ggatagtata gtgctggatg caaaggcgaa
    15841 ttctttctta gaacggacta tttccgactg ttcctccatg gtatttcctt tcaattaact
    15901 ttctttttta cctaaaacca gtgatttaac gggttcaaag ctggttcggt gaattggggt
    15961 aactcctagt tttgtgaggc cttccagatg tttagctgtg ccatatcctg cattagtagc
    16021 gaaatcatag ccagggaact gctgatcgta ttccttcatc aattcatcac gtgttacctt
    16081 ggctactata gatgctgctg cgatagagag ggagttggca tctcctttga taatggaggt
    16141 ttgtgaaatg ggcaagtcca gtttcatggc atcaatcaaa aggtgctctg gttgagggct
    16201 gagctgggag attgcttctt gcatggctag tttggttgct tcatagatat tgacttggtc
    16261 gatgacctga ttatctatga taccaattcc aatcgacaag gcttggtctt gaacgacttg
    16321 gaaaatctcc agatgtttct ttttaggaat tttcttgctg tcgttgagac ctttaatctt
    16381 acaattttta ggtaaaataa cggccgcagc gactacagga ccagcaagag gaccacgacc
    16441 aacctcatca atacctgcaa ttaaggtcaa tccttgctta taaagttctt tttcataaga
    16501 aagcatggat tccaagcgca aattttcatc taattcagct tgaatggctc ttttatgctt
    16561 gctgatttcc ttttgaactc ctgagcgatt atccttttca agatctaaaa aaatagggct
    16621 ttctaactcc ttgactgtca caaggagttc tttgatttct ttaatcgttg ccatcgaggt
    16681 cttccaatgt atctaaggta tagttaccga gtttgccatc acggacttcc ttcacgaaga
    16741 gactgtaaaa acggtcatag tcatcacgga aaccgagggc gcgggtcata tccataataa
    16801 tcacaggcgc ttcttcttca attttcattt gtttgaagcg ttcagccagc ttttctggat
    16861 aatgttcttt gaaataattg ataccaaaaa tggtaacctc atccatagga agcaactgat
    16921 ccttgatagc tccagtcaat gccaacttaa gtgcaacagt ttcatcctca aacttaggcc
    16981 agagaatccc cggtgtatcc aagatttcca gatctttatt ggttttaagc cattgttgac
    17041 cttttgtgac ccctggcttg tttccaacaa cagcaatctt tttaccagcc aaacggttca
    17101 tcagagttga tttaccagcg tttggaatcc caataatcat ggtacgcaag gtttcaatct
    17161 gaatcccacg ttctttctgg cgagcaatct tatcagccat gagcttcttg gccgcatctg
    17221 ttacaacttt tacagtcact tgctctttgg agttgatagc tagcgtctgg attccttgtg
    17281 attcaaaata ctgacgccat tccttggtca ttgctggatc agccaagtcg gccttgttta
    17341 aaatcaagag ttttggttta tcaccaacaa tcttggttaa cataggattt tgactagata
    17401 gaggtaagcg tgcatcaact aaaatcgtca caaaatcaac aaattttaaa ttctcctgca
    17461 cctgtcgacg agctttagac atgtgaccag gaaaccattg aatagtagcc attgagtttt
    17521 ttcctttcat agcaaggatt tagatcccct attttatttt actattgttt gaacatagag
    17581 agttttcaaa tgaatgatgt ccaaacagtt cttgttttat gacatagaaa tctactttat
    17641 attatatcat gccttcttta ttttgtccga tgatagcctt ttaccctata tagaaaaaag
    17701 ccatcaaatg atgacttttc agagggagca aaaaactgtt cccgtcatat tatgaaccaa
    17761 aagaactaaa ctaccaacag ctaataggcc taaagctgta agtgcagaag ttgatgaacc
    17821 tgttgcagga agagtttcct gctgataaat aagagcataa cttacttcat tatcagtagg
    17881 aattcctgct tgaatcttgg tttgttcttc atcacctttt taaactttat ttcctaatag
    17941 taaacactat tttttattct caaaaattga acttctaaaa tcatttgttt ggtcaagata
    18001 ggctttaaag attgctctct tcaacttatg aacggcactg tcgatttctg gatcataatt
    18061 actccaacgt ggaccttcag catccttgag aacagcttgg tccatgaggg cttgcaattc
    18121 atccacatta ttgatagtct ttgtgccata gcttggccat ggttttgtcg gatctttaaa
    18181 ggtcacctgc ttcaagtttc caaactgatc cactcgttct tggtacatag ctgtcttaaa
    18241 ttctgcccaa gttttatact taccgtcaaa tacctttttc aaaacaagct catcggtcac
    18301 caagccccgt tctttaccat aaagattgat agtttgccct tgttgtttag catcttcttc
    18361 gtattggttt gagatataag gtaccattcc atccttaaag cctttggcag ccaaaagttc
    18421 gtacgcaatc ctacgtccca taaggtcccc tggtgtgcct ttctcactgc tgagagctga
    18481 atagattggg gcaaagagtt tgatggtata gtagccattt cgttcatagt cgccagactg
    18541 gtactcacga gctgataaga tgttgtggtc aatcaaactt tcaaaactat tcaggtttcg
    18601 ggcctctgct tctgtcaatt ctttgactac gttagtggca taaacctcat ttccatctgc
    18661 tggatctttc acatacttgt tctcaatttt tctgagagcc gccattttct ggtaaacact
    18721 tagtttatta acgattgact gcccttcgag atattccatc atgtagataa ggtcaaacat
    18781 gttatggaca tagttctgaa gatctgctgc gttttggaat ctctctgtcg gatccaagac
    18841 ttgaagacgg gatccctctg tactatctga ttttgagtgt ttcaagatag aattaatggt
    18901 aatggttgcg tcacttggtt ggtcaggggc ttgcaataag ccttttgcga agaactctgg
    18961 tcccaagcca tttcttcgac catagccacc aaggtaaata tcctgatccg aatcatgggt
    19021 catttcatgg gtataagtaa tggctccatc cttgtccagc atgcggtaag acatatagta
    19081 gacgccgtcc cctgtagcgt aggctccatg ctggttgtgt actaccttat tgccaactgg
    19141 tccaaagaag ttcttcatag ctggattgga gctatcaaac ttagcctccg ctgtagcttt
    19201 tcctgatgtt gtgtcatccc caaacttata ggcatcataa aggagaatcg tacgatagag
    19261 tttttcgcgt gactgttcat ctaaaatacg ataccaataa tcgtagtgat ctcgctgacg
    19321 tttggctgtt tcacgcgcat tttcttcaac aaaatcattg agagccttgc ccgctttatg
    19381 gtcactactg cggtagcgat cataagctcc aaatcctaga ctagacatgg tcgagatgac
    19441 aaatacggat ctctctggca aggtcaggag aggcaagacc atattgcggt atttccatgt
    19501 ggcactcgtg atacgatcat aaacaccgat agaatacttg gtgccagcta acccttgctt
    19561 cgttttcacc tcttcgatag tggatttttc ttcgacaatg taagccttag tctctgattt
    19621 aaaccagtca ttattgcttg tatttggtaa aaagactttt cggtaatgtt ccagcgtgct
    19681 aaacaaatct gtcgttccat gttgactggc aagactgata ccataagtat cgacattatt
    19741 cttagctaga agattgttaa agccagattt acctaactca atcagagtat ctagtgggga
    19801 agtatttcct ttaccaaaga agtctggatg atacataact aggtctttga cattcacttg
    19861 accatagtta aagttatacc aacgttctaa ataagttaaa ccgagtagca aggcttcctt
    19921 attattcttg attttatcta caagatagcc tctggtgact gagttatcgc tagctagacc
    19981 ggcatctgct gacaaaagtt ttttcaagct gtctcccaga ttttgttttg ttttggagaa
    20041 ctggtcttct aaatagagct cggttaactt aacttctgga gaaataccaa gtgtctttct
    20101 gatagcatct gactggtaat caagcttctg caactcaggc aagacttcct taacaataga
    20161 gtctcgatca tataagaatt ggttaggagt gtagagaagt cccgtattac ctagattata
    20221 ttctgctaga ttggcaaaat cagtctggta cttgagatca aatttctcag atgaatggtc
    20281 attatagtga agtaagagtt tgtttgcagt ctgtttgttg gaaacaatat ctgtgattac
    20341 ttggtcatcc ttcatcataa ctgcagataa gagttctttt tggtataaga gactgttctc
    20401 cttaaccagg tttccgtatt tgacgatggt tgacttatta taaaaaggta acaatttttc
    20461 aatgttctta taaaccaaag cacggttagc ttgataatgg gctatcttag aaaaatcact
    20521 gtctttcttg ccacttgttg agaggggttc caccgttggt agagtgagag gatttatttc
    20581 tgcttttttg cttacaattt gagaagcatc tagcattgtt cctctttctt caaaggattc
    20641 cttgctgacg acctcatcct tctctaaagt gacattgaaa actctattag ccttactact
    20701 aaatgtatta gctaccttca ttcctgtata gtggtatcca gtgatggcat ttccgtttgt
    20761 tacattgaca tcgctaagaa cattggtcaa ttcaccagcg tgtttttcct caccagaagt
    20821 tcgatcccac aaatatcctg ccacaccagc gactttacca aagtgcttga cattattaat
    20881 atcaccttcc gcataactgt tctgtatatg cgcatcttgg tctactagac ctgcaagacc
    20941 accgacagtc tgatctgacc tattggtgtt tgacgaaatg gttactgtcg ctttggattt
    21001 agcaatagac gcattttttc ctgttaaatg accgaccaga ccgccaatat tgtaggcatc
    21061 agtcgtttca taggtattga caattcttcc cttgaaactg ctctctgcga tgcttgattg
    21121 gtctgcctta gccaacaaac caccgacacc acgttctcca gccagaacac catcaacatg
    21181 aacttgttta atctttgtgc cattcgtagc ttcatttgct agtgaaccaa tatcattttt
    21241 ccctgaaata gcaacatttt ttagactcag tttttctact gtagcaccac tcaagttttc
    21301 aaacagaggt tttttcaaat tatagatagc ataattcttg ccatcttttt caccgattaa
    21361 acgaccagta aaggtgtcct tgatatagga tctttcatca ggaccaagct ccacttcgtt
    21421 agcattcagg ctggccgcta aatgataggt tccagaggga ttttggttta tagctttgac
    21481 caggttgcta aaggaagtaa agtttgtatt ttcctcttta gccttcttat cgaggtagaa
    21541 ggtgaaattg tcgacatatt tattttctag ttcttgctgg agtttctcag ctttggctgt
    21601 gattttataa actgactgcc catttctttt ctcttcagtt attgaagcta ctggtatata
    21661 gacatcttta aatgctgaag attttacctt gacaaagtaa gtatccgtat tctctggaat
    21721 tccatccaat gaaacatgtt gtctgtaggt tccattcgtc tgactataca actcaatatc
    21781 agaaacattt cttaattcga gttttttctc tggttctgtt tgtccattct ctgatgttgt
    21841 attacctgaa ttttctggtt ttgttgcagt tccgtttttt tctggttgat ttgattcttc
    21901 aactggtggt tttgaatcac taggtttatt ggatacttct ccagtatttt cgttagctat
    21961 tttcccagag tttgtttgtg tttcttctgc aggttgaact ggtttttctg tttcttgatt
    22021 tgaggtacct tctactgtgc cttcatttgg atttactgga acttcttcta cagttttttc
    22081 tgaattttca tttttagagt cattatgttc tggtttattt gattctccaa ctgaggttgt
    22141 cgaatcacta ggattactgg acacttcccc agtattttcg ctagatgtat ctggtgatac
    22201 tttctctgaa ttcgttgttg attcttctgc aggttgaact ggattttctg cttcttgaat
    22261 tgaggttcct tctgtagtac cttcatttgg atttactggt gtttcttctg ttggttttac
    22321 tggaacttct tcagtttttt ctggaccttg ttctttggtc ttctcaaccg gagtttcagg
    22381 ttttacttgc tcaatattac ccttatattc tggcaatggg gctacctgct ctggttcacc
    22441 tttatcactt accacagtat ctggcgactc tggttgaacc tcagtctcac ctttgtcggt
    22501 cacaactgct tcgggtaatg tcggttgaac ttctggttcg cctttatcac ttactacagc
    22561 ttcgggcaac tcaggctgaa ttgcgggttc aacaatagat ccagactgta cgtccttatg
    22621 ttctacacca gtctcaggtt gttcctttat aacttgagtt tttttagtac ctttttcgac
    22681 tattcttgga ctaggcgcag tcgttgaagt tgaaacaatt tctcgcgaaa cttcttcctt
    22741 gtttacagag aatattctga cgatttcaac tttcttacct aatttacctt cttgttttac
    22801 tcttacagtt ccttcagcta aatcaggatt ttcttgaatt tcttcttgaa aatctatttt
    22861 tgtctctata gtttcctcac gatataagag ttcaggtttg ttcaattgac ctgataaaac
    22921 ttcatcctgt ggatttaatg tatttacccc agtcttttct tttggagaaa tcttctcctc
    22981 tttcttcgtt tctagattct tatgttcggc taattgttct tgagaatctg aagattgttt
    23041 ctcttctttt cttggattga ttaattcagt agagaaaggt ttttcaacta cttgaacttc
    23101 tgtcggctta gttgaagaaa caggtgtttg ttcctgaata gcttgtactg ttgatggatg
    23161 gtctacaaaa tttggtgtaa cattataatc caccttttgt tgttttgtag gagtggctgc
    23221 tgatttttct tgattactta cttcaaaatc agaagtcgtt tttccctctt tgatatatcc
    23281 aatataagtg taacctgaaa tctctttagg aagaggtaat ttttctccag aggtcaattc
    23341 atagtccgta ttgtaattta gcaaaagatg attttctaaa gcatggactg aaactaagac
    23401 accatttccc attcccgcaa ccaatactaa atgtaatacc gttttattct taaccttttt
    23461 cttggaaaca gcaaaaatta aaattcccag agcagctaag ctagcaccag caactagggc
    23521 ttgcctctca ttcttgcttc cagtatttgg taattccgcc agttgatttt gagaatttaa
    23581 cttataaaca agataataag tttcatcatc attctccacg tatgtcggaa tatcatagac
    23641 aagctgcttc ttttcttctg atgatagctc tgaatctgcc acatatttat agtgaactcc
    23701 cgcagtttct tgagcatcca cagatgaact agctaataca gacataaaaa ataaacttga
    23761 aatcgttgca gatacaagtc ctactgataa ttttctaaat gaaaaacgct cttgtttttc
    23821 accaaaatac ttttccatta ttcctccttg aaataaaatt tatatatgtt acaaagacct
    23881 ttattatatt agtgtattat ctattatcta tagaaaaggc agtatacctt aattatactc
    23941 ttaatttaca aaaaagtctt aaaattgaga tgcgctttca tactttgttt tatattattt
    24001 gggggtacaa taacacctac catgaaattt acacggtagg tgttactcat atcactaatc
    24061 gttctaaaaa tggtttgagg cagttgagga gaattccttc tatccagctt ccttgtgctg
    24121 atgagcgatg gtcttcctgc aggctttttt ttagaaaatc tcggacttgt tctggtgcga
    24181 tttcaaattc aaaggctttc attttataga aaaagtcgat gagatgatct gacaggtatt
    24241 cagttgaaaa gggtacttca ccactttttc gatattctaa taagagtcta gaaaatcgag
    24301 ctttttcttc aggaagctca cgaaaatagg aattgaggat ccaagtctgc ttctgttttc
    24361 tttcaattgg atcctgactg gcaattcgtt ggtctttttc cagctctttt tggtattgtt
    24421 tggccttgat agctcgttct gctctatttt taccaaaaag aattttttcc cacttgcgtt
    24481 cttcttgagt cagggtctct gtaaagccaa agtaatcttg ataagcacgc tctgcgggtc
    24541 ccatggctag aaccagattg tctgcatatt gcttggcgat tttatccctc ttcttgcgtt
    24601 ctttctctgc ctggatacgg agttcttgtt cgtagtcaat tttctccttg cctagcttga
    24661 caaggtagag ttggtcatcc gatttcccaa gtaaaaaggg tttgatacac ttttcaagga
    24721 cttcttccat ccgagccttt ttctttggtt ccgccttggt ccaacttcct ccctgaaaga
    24781 cttctaggaa aagctggtag tctctctcag gcgcaaattg attgccacga ttgggtttga
    24841 aaacaccttt ttcccagagc cattttagaa gtcgctcgtc aaagtcactt ttattgacct
    24901 tgattttttc ctttttctga gcttttctgg ttagattttc aacctttctg agcagttttt
    24961 cttcctcttc caattgctgg tcaaggggca atcgatgaaa atgacgaaca cagtcgctac
    25021 caattggaaa gaggcgttgg cctgtgacac cgttaaagag ttcataagcg tatttgatgg
    25081 catttcgaca gacacaattg ctacggccaa taccgttaaa aatcaaggaa acttcattcc
    25141 attccttggt agcttgttcc caagtatccg ctttcgaagc ctgtaaaact gcatcgtgca
    25201 gggattttct aactggaagt gtcatgaggt ctcctttcta atactcaata aaaatcaaag
    25261 agcaaactag aaagctagcc gcagtcagct caaaacactg ttttgaggtt gtagatagaa
    25321 ctgacgaagt cagtaaccat atatacagca aggcgaagct gacgtggttt gaagagattt
    25381 tcgaagagta taagttatac ttttacaact tgaacctcgt ctttaccgag taaaatcaag
    25441 tatttttcaa tattttcaat cgaataggct cgtgataaag cctcttcgta tagagctaac
    25501 tgaccacgat agcggtctac gagttgactt ggttcatcat agcggtctgt cttgtagtcg
    25561 aacagaacaa ttttgttttc gtaaagcaga tagccatcaa ggataccacg gacaacaaag
    25621 tcttcctgac tcttttggtc tcgtttgagc atggagaaag gttgctcgcg atagagatgg
    25681 tcggtattag caagaatttc ctgaccgagt gctgtgtcaa agaaagcaag aattttatca
    25741 agattgatct tgtctctgac agcttggcta gtttgaactt gtttgagtgt ttctgttaag
    25801 ctagcaaggg ttagttgctg gctgaggtca attatctgca tgagttcgtg agtagcacta
    25861 ccaatctcag ctccagttac cttttctttg gttgaaaaat ctggcaaatc gaagctgatt
    25921 ttcttgccta ctgactgacc ttgaccagca atctcgacac cttccatatc cataactggt
    25981 tcgtagaatt tcttgatttg acttggggtt tgaacactag gaagttcaat agctgcgcgg
    26041 tgaagagtat tataaacttc cacctccttc agcatttcca gagcttcttt gatggtatct
    26101 gactgacgat tgtctgcttg ggagctatct tggagaggac tcttggtttc caactctccg
    26161 atagcttctc tggtcaactg atcttcgtca ataaaacgat aactaaagtt gagcttgtcc
    26221 ttagtaaaca ctttactgat agcccaaagc caatcttgga aattccgtgc ttgcagtcta
    26281 gtattgctat ttagtttccc atttttggct gctgggtatt ccttggattc cagcttttca
    26341 cgagaaccct tgccgacaag atagagcttt ttctcagccc gcgtcatagc aacatacagc
    26401 aaacgcatct gctcagaata gcttgctagc tgtaattcct cttcgttctg cctataggtc
    26461 agactaggaa tggagagttt gatggtttta ggatagtggt cttctactgc ccctgtctcc
    26521 atcttggcaa tatatttgac accaagacca ttctgacgac tgagaatgac ttctgacata
    26581 gagtcttgct tgttgaaatc ttgatccata ttgaggataa agacgtaagg aaactccagc
    26641 cctttactct tgtggatggt catgagctct actgcatctt ttggcggtgc gacggccacg
    26701 cttgccaaat cgtgctgggc ttctaagact tggtcaatca tacgaataaa acgcgacaaa
    26761 cctttgaaat tgctcttttc aaattgatca gcacgcagtg ctagggcata gagattggcc
    26821 tgcctagcag gaccattcgg caaagcccca acatagtcat aataaaaacg gtcgttgtaa
    26881 atcttccaaa tcaagtcata gagagagtgg gttttggcat acaagcgcca agaagctagg
    26941 atatccatga attgctttag tttttcaact agagctgtgt gaatcaagcc tttttgacta
    27001 cttgccattt tttgtgcatt gaccagtttc tcatagagat tttcgtggac tttatcctct
    27061 gctttctgaa gggacaaacg tgctagctca tcctcatcaa aaccaaacat tggagacttc
    27121 ataagggcaa ccaaggcgta gtcttgcagg ggattgtgaa tgacacgaag agtgtctagc
    27181 atgacttgca cttctaggga ttggagataa ttgttttgct ctccgtcagt tttgacagga
    27241 atcccgtact cagacagggc gaggagaatc tggtcattac gactgcggct ggaggtcaga
    27301 agggcaattt ccttaaaggc aacacctttt tcttgatgaa gtttcagaat ctccttgata
    27361 actaagcgca tttcgcctgt tagtttcgtt tctgtttgac tctcttcttc ctcacctgta
    27421 tcgtccttgt cgtagaggag aaattctgcc ttgttgtctg gattgggagt cagtttggta
    27481 ttggcaaaaa caagctggtg cttgttatca tagttgattt caccgacctc ttggtccatg
    27541 agacgttcaa agacatcatt ggttgctgac agcacttctg aactactacg gaaattttcc
    27601 ttgaggagaa tgagcttgcc ttcttgggga ttttgcgcat agcgttggaa tttctcattg
    27661 aaaatctgcg ggtctgcctg acggaaacga tagatggatt gcttgatatc tcccaccata
    27721 aagcgattgt ggccattaga caacaattcc agcatccgtt cttgaatatg gttggtatcc
    27781 tgatactcat cgaccatgac ttcatggaag cgctcctgat aagactcacg aacttgtggg
    27841 aaattctcta aaatctcaat ggtgtaatgg ctgatatcag cgaattcaaa ggcattttcc
    27901 tggcgtttgc gctgacgata agcctccaca aaatgactca taaaggtttg gaaggtttta
    27961 gctagtttcc atgtgtcttg atgataacgt tcttgatagt cgagaatggt tatctggtct
    28021 gataattgtc ctagtttagc aaactgggtc tttctctctt cgttgtaggc atcagccagg
    28081 ggcttcaaat cagccttacg actggcatta gtcagagctc gaccgttttt ctccttagag
    28141 atggcgacaa cacgcgcaag cactgcctga taagcctgac tatcggactc ctgatttagg
    28201 gagcaaattt catccagaat taactgaaca ttttctaaat aggcagcctt tgcaaactcc
    28261 ttggcatcgt tatccagatg gtaacggaaa aagctttcca aatcccaaag ggcttgtttg
    28321 atttgctcag tcagtttttc tttttcactg gtaaaatcag ctttctcaaa tcctttgagg
    28381 aaagattcac tcagccactt ttgaggatta ctggtggatt ggaggaagtc atagatttta
    28441 tagacctgct ggcgcagacc ccgttcgtcc ttgccacgcc cagcaaagtt tttcagcaaa
    28501 tgactaaagg tctctttctg tttaccttgg taatgcgctt caaagacctc atgaaagact
    28561 tcgttttcga gaagaagttg ctcgctttgg ttttgtaaaa tacggaaatt aggtgcaata
    28621 tcaagcagat aaccatgttt gccaaggaat ttttgtgtga aagagtccat ggttccgatg
    28681 gcagcgtttg gtaggtctgc caactggcgt cccaagtgtt gtttgaggtc gacatcatct
    28741 gtttcttgga ttttcttgct gatttttttc tctaaacgtt ctttaagttc agttgcagcc
    28801 ttgacggtaa aggttgagat aaagagttga gaaatttcga caccacgcgc caattggtcc
    28861 agaatgcgct ctgccatgac aaaggtcttt ccagaaccag ccgatgctga gaccaggata
    28921 ttctgaccag aagtgtagat agcttcgatt tgctcggcag ttttcttttg ttccttgctc
    28981 gaatttgctt ctgcttcttg cagtttttga atctcctcct cacttaaaaa gggaataagc
    29041 ttcatcgatt caactcctct cttatttttt caagccaagc ttgcttgagt ttttctccga
    29101 ccagacgctt gccatcagct aggtccaact tttctaggaa acgggcttgg cccagatggt
    29161 aattggcttc aaagcctgta atagcctgat gttgctggac gtatggggca atgcttctgc
    29221 cattttcagt ataaggattg atggcgaacc ggcctgctaa aatcttctca gcagctttct
    29281 tgtaaagata ggcattgtag tccagtagga gctgaaattc ctcatctgtc agttgattcg
    29341 ccttgttttt gttataaaat tcgcctaaat aactgctttc tttttccaag aagagccctt
    29401 ggtatttcat agatttgctg gcttctacca ctgctcctgc cagacttttt accgccatca
    29461 gagattggac aggttcagcc atttccaagt acatggcgcc gaaaaagttc tgctcccctt
    29521 ctctttttag ggcagcaaga taggttggta actgagaatt gagcccatta aagaaatgag
    29581 gaaactggaa ctgagtcaga ctggatttgt agtctactac tcctatcgct ccattagctt
    29641 tcaaacggtc aatccggtcc accttgcctc gtacaaagac actgcgtcca ttgtctaatt
    29701 gaataaaggc ttggtctttt ccaccaaaat ttgcttcttc tttgattgtt tcgatggctg
    29761 gattgtgtcg gagaatatgt ccagttgtcc gtgcaacatc aagcaaaact tccttggtaa
    29821 actgggcttc caaactttct tgataaatag cttcaaattc gcgttcttga ctggtttctt
    29881 gaatagcttg ttctagacgt tggtcaaagg aatcttcatt aggcaactgc aaggcgcgtt
    29941 caaagatacg atgcaagaaa ttcccgtgac tacgggcatc aggacgcaaa cgtaattcct
    30001 cctgcaagcc taaaacgtag cgtaggaaat aactgtattc attgcgataa aactctgtca
    30061 accccgacgt agacaggtaa aactcctgtt tggcaggata gagagcttgc aaggtgtcct
    30121 tggctaaggt cttgctgctt ggactgattg ggatggctgg attttccaga ccttgctgat
    30181 ctagtttttt acctatgaca cgcgacagaa ccttgacaaa agtcaaatct tgctcagtat
    30241 cgctcatctc accctgctgg tgataggcaa ccagactaga caaaagactg tgataggacc
    30301 ccatatcctc cttagacagt cctttgtgat tcatcctctt ctctctccgc ctaaatccaa
    30361 aatggatcaa ctcttgaaga taggcagatt ccttactttc actttcgtta aaaaggcttg
    30421 gagccgacaa gaacaactgc ttacgagcag aattgaccaa ggaaagcata gtgtagcgat
    30481 ttttcttgag attttcactg ctggcaatca gtaattgaac gccttcttcg gtcgtttggt
    30541 ttaggttttg cctttcttca tctgtcagaa gactggtgtt ttgagaaatt tttggtaaat
    30601 tgtcctgagt tagtccaata gcatagacaa agtcagcagt caatggtgca atcaaatcgt
    30661 aactctgcac cagaacagtg tccactgttg ctggaatggt acggtattgg gacaaactca
    30721 ttccagaatg gagcaaggct aggaagtctt ccagactaac ctgtgaacca gcaaaaacag
    30781 tcgcaaattg ttctaaaaca tggcagaaag ccttccaaac ttcggcttgt ctttcctgtt
    30841 ctacagcttc caaagtggtt gtcaaatctt gtaactgctt ggtcacagct ccttctttta
    30901 gaaagacact ccatttttgt aggaggtttt cagccttttg ttttcggctg gcaaagaggg
    30961 tttcaagagg tgctaaaatt ctcaggcgga ggacattcaa acgctcaaga ttaaattttc
    31021 catggtggga tttggtgaaa gtttgctgaa aggctggcaa gccattgata ccaagatagc
    31081 ggatatattg ctcaaaagca tcaatatcag actgactgag gtcagtatac aaatcagttc
    31141 taagaagatt aatcaaatcc tcctgacgaa aacggtaacg ttttaaagct aaaatagact
    31201 cgacaaactg agtcaaggga tgatgagcca tggcttcgct tctaccaaga taaaaaggaa
    31261 tctgatactg gtcaaaaatg gttttgagag ataactggta agaagctaca tcccccaaga
    31321 gaatacgaaa atgcttgtag ctcaggtctg agttctcatg taatttctga cgaatgctac
    31381 gggctactag ctccaactcc tccttttgcg tcaaacaaga ccagatttgt aaattttcac
    31441 ggtctttctc atcgacatcc aaagcgagtt ctgaaaagtc ataagaagac tccaacaaac
    31501 gagaggcctt gtcaaaacta tccatcttct catgagtttg agaacagtcc tgagcaggcg
    31561 tttggtattt agaagccaga tgatggagaa attttacgct ggcttggtag agattgccct
    31621 cgctaaaagg actggtatag gctttcttac tagcataagc cccgataaca atctcaacac
    31681 ctttgccgtg aagtaagtcc acaacccgct cttcctcagc agaaaaacga gtaaagccgt
    31741 caatgaccaa ggtgatttga ttaaaatcac tacttacctt gtcattctca atagcctcaa
    31801 tcaaatggga caactgactt ccctgggcta actgaccttg attaagatag gctgttactt
    31861 tctcaaaaat caagagtaaa tccgccctct tatcctcatc tgttaaattc tccaagtcca
    31921 aaaaactcat ctgagatttg gtcatctcat ggtaaagctc aattaactgc tggatcaatt
    31981 gaggatcctg cttaatagcg ccataaacac gcaagtcctt gggatcgagt tcggcaaggc
    32041 atttgtaaaa ggccaaccca agaccgatat catcaagagt agttttaact ggtaaatcat
    32101 tcaagaccag ataacgagcc atttgagcaa agcgcgtgac ggtaatcgaa aaagaagcct
    32161 gctgggacaa gcattccagc acggcgcgtt ccttttcaaa agaaagagag ttgggggcaa
    32221 tgtagaagac ccgcttgcca gctgcaacta gctcttctgc ctctcttgtt agaatttctg
    32281 tcaaagaagt tcgaatatca gtataaagta atttcatctc agcctcgttg gaatttttca
    32341 tcaccctata ttataccatg attagcctcg taaatctgtt aaaatattta ggccatcctt
    32401 tcttttcttc atcatctgct aaatcttaaa tacttagctt tacttgtatt agatagaata
    32461 agtctggcta ctgaaaatca cataataaaa aagcctcggt aacaaggctt tgagttttat
    32521 gattgtttct taggtacgga atacacttca atgtgttgtc ccagtatctt aatgtcgact
    32581 ggtagattgt ctgatttatc gccatcaaca tcggactcta attcgatatc agaagaagtt
    32641 ttaatattac gtgcctttat atattcaata ttcttgatag aatgattgaa ctatagtaaa
    32701 ttgaaactat aatagtacac cgtggatgct aaaatatttc tagaaattaa tttgatttcc
    32761 ctaatcaagc tgttcgtatc ttatttcaat cta
//
LOCUS       DAVBCR010000018        32551 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCR010000018 DAVBCR010000000
VERSION     DAVBCR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.018"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ508_000751"
     CDS             complement(322..1170)
                     /gene="dprA"
                     /locus_tag="VQ508_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2489783.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1268..1957)
                     /locus_tag="VQ508_000752"
     CDS             complement(1268..1957)
                     /locus_tag="VQ508_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2489784.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1969..2847)
                     /locus_tag="VQ508_000753"
     CDS             complement(1969..2847)
                     /locus_tag="VQ508_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2489785.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ508_000754"
     CDS             complement(2837..3706)
                     /gene="licA"
                     /locus_tag="VQ508_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2489786.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3723..4745)
                     /locus_tag="VQ508_000755"
     CDS             complement(3723..4745)
                     /locus_tag="VQ508_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2489787.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4750..5457)
                     /locus_tag="VQ508_000756"
     CDS             complement(4750..5457)
                     /locus_tag="VQ508_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2489788.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5793..7280
                     /gene="tacF"
                     /locus_tag="VQ508_000757"
     CDS             5793..7280
                     /gene="tacF"
                     /locus_tag="VQ508_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2489789.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7290..8093
                     /locus_tag="VQ508_000758"
     CDS             7290..8093
                     /locus_tag="VQ508_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2489790.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8095..8904
                     /locus_tag="VQ508_000759"
     CDS             8095..8904
                     /locus_tag="VQ508_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2489791.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ508_000760"
     CDS             complement(9179..12355)
                     /gene="carB"
                     /locus_tag="VQ508_000760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2489792.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ508_000761"
     CDS             complement(12667..13746)
                     /gene="carA"
                     /locus_tag="VQ508_000761"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2489793.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13796..14719)
                     /locus_tag="VQ508_000762"
     CDS             complement(13796..14719)
                     /locus_tag="VQ508_000762"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2489794.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ508_000763"
     CDS             complement(14738..15259)
                     /gene="pyrR"
                     /locus_tag="VQ508_000763"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2489795.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ508_000764"
     CDS             complement(15470..16099)
                     /gene="nth"
                     /locus_tag="VQ508_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2489796.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16099..16641)
                     /locus_tag="VQ508_000765"
     CDS             complement(16099..16641)
                     /locus_tag="VQ508_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2489797.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16820..17137
                     /locus_tag="VQ508_000766"
     CDS             16820..17137
                     /locus_tag="VQ508_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2489798.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            17376..18935
                     /locus_tag="VQ508_000767"
     CDS             17376..18935
                     /locus_tag="VQ508_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2489799.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ508_000768"
     CDS             complement(18979..19878)
                     /gene="htpX"
                     /locus_tag="VQ508_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2489800.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19880..20440)
                     /locus_tag="VQ508_000769"
     CDS             complement(19880..20440)
                     /locus_tag="VQ508_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2489801.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ508_000770"
     CDS             20534..21247
                     /gene="rsmG"
                     /locus_tag="VQ508_000770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2489802.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            21501..22784
                     /locus_tag="VQ508_000771"
     CDS             21501..22784
                     /locus_tag="VQ508_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2489803.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ508_000772"
     CDS             complement(22979..24550)
                     /gene="ffh"
                     /locus_tag="VQ508_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2489804.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(24562..24894)
                     /locus_tag="VQ508_000773"
     CDS             complement(24562..24894)
                     /locus_tag="VQ508_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2489805.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(24985..25362)
                     /locus_tag="VQ508_000774"
     CDS             complement(24985..25362)
                     /locus_tag="VQ508_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2489806.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            25434..26738
                     /locus_tag="VQ508_000775"
     CDS             25434..26738
                     /locus_tag="VQ508_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2489807.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            26751..27557
                     /gene="yidA"
                     /locus_tag="VQ508_000776"
     CDS             26751..27557
                     /gene="yidA"
                     /locus_tag="VQ508_000776"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2489808.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            27600..28598
                     /locus_tag="VQ508_000777"
     CDS             27600..28598
                     /locus_tag="VQ508_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2489809.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(28765..28836)
                     /locus_tag="VQ508_000778"
     tRNA            complement(28765..28836)
                     /locus_tag="VQ508_000778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28801..28803),aa:Arg,seq:cct)
     gene            complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ508_000779"
     CDS             complement(28879..29226)
                     /gene="rplS"
                     /locus_tag="VQ508_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2489810.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ508_000780"
     CDS             complement(29345..29674)
                     /gene="crcB"
                     /locus_tag="VQ508_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2489811.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ508_000781"
     CDS             complement(29668..30042)
                     /gene="crcB"
                     /locus_tag="VQ508_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2489812.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(30039..30305)
                     /locus_tag="VQ508_000782"
     CDS             complement(30039..30305)
                     /locus_tag="VQ508_000782"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2489813.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            complement(30421..30864)
                     /locus_tag="VQ508_000783"
     CDS             complement(30421..30864)
                     /locus_tag="VQ508_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2489814.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            30968..31903
                     /locus_tag="VQ508_000784"
     CDS             30968..31903
                     /locus_tag="VQ508_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2489815.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            31957..32241
                     /locus_tag="VQ508_000785"
     CDS             31957..32241
                     /locus_tag="VQ508_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2489816.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(32298..32516)
                     /locus_tag="VQ508_000786"
     CDS             complement(32298..32516)
                     /locus_tag="VQ508_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489817.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
BASE COUNT         9878 a         7017 c         5944 g         9712 t
ORIGIN      
        1 tttcccttat actcaatgaa aatcaaagcg caaactagga agctagccac aggttgctca
       61 aagcactgct ttgaggttgt agataagact gacgaagtca gctcaaaata ctgttttgag
      121 gttgcagatg gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa
      181 tcctctactc tactaatcca cttagaaaaa acgcatatta tcaagtgtta aaaacttgct
      241 aatatgcgtt ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta
      301 gaattctagc ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt
      361 gaccaatttt gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat
      421 gctaccagga atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag
      481 actacctgaa cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat
      541 gcgattacgg gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag
      601 aaccagatgg tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac
      661 atccagtcct gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg
      721 agctgctgtg tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc
      781 ttgaatgact ttttcaactg atttagctcc ctgtttgcta caagcacgac tgcccacgac
      841 cgctaccttc gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc
      901 atcatatatt tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa
      961 tttttgaaac tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac
     1021 ggctggatta cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac
     1081 attttcaccg tattctagaa ctttcaaaat ctgttgattg gtcaaacctg atttttttaa
     1141 cttatagatt tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct
     1201 atctattcgt aaaagttcgt aaaaaatcaa aaaaacatcc gactattttg tcagatgttg
     1261 gaagctttta attttcgttt ttaagaattt cttctaattt atgatagtct tggacactat
     1321 caatctcata aatgctattg ccttctaatt cttcaacata gacatctagc tctttgatgt
     1381 tatccttaac catattgtcc caatagagat caacaaattc gccgcttgca taagccttat
     1441 cgataaagct aacaattttt tctgctgttg gagcatccca gaaggataca ccactaagga
     1501 tgcgacctgc cttgctatca acaataatgt tttgaacctt gtagtcatct ccatagacca
     1561 agaaccattc gttggtacaa tcttcacgat agacactaaa ataagtagaa cgtgtcaaat
     1621 cattgcggaa catattttta aagagatagt tatcggcatc gataacataa ctattggcta
     1681 attcttcttt tacaagatag agagagtaaa agttattgta gtcagcgtat ttatcattga
     1741 aaacgagacg aacaccgtat ttctctttca agtaatcgaa ttgttcttta agataaccaa
     1801 caatgatgat gatgtcattg attccttttt ctttgagaaa ctcaatttgg tactcaatca
     1861 aaggtttttg attaacctga accaaggctt taggggtatt ttcagtcata ggacgcaagc
     1921 gagttcccaa tcccgctgct aagatgatgg ctttcacacg aatctccttt attctttaat
     1981 aataatataa actccagcaa tgacgacaag tgacgtcata atggtcagca tatctagcgg
     2041 tgcacccaag aaaacaactg caaacaagac cgtccatact acatagctca cgttcaagcc
     2101 tgtagccttg gctggttgca agcgattgat agcgatataa taagccaagt aggaaatcat
     2161 atcaaaggct gcaaaaacaa tcatgagacc tagcaattgt ccattggcta ctgcagtaaa
     2221 tgactgatga gagaagagca caatcacaag ataggacaag aacgaagtta cttgacggat
     2281 taagagggct tcgatttcac tcaattcact ttccatggca aaagagctaa gaacactctc
     2341 acttccccat gcaatagcac aaaccaaagc acaaagaatc ccaatgtaga aagaattaac
     2401 ctgttcaact ttataggtct gggcaataat ccctccaata atcaagacaa tcccaaatac
     2461 agtatttttc gaaatcttgt gcttcaaaaa gaagaaagcc aatagaactg aaatcgcagg
     2521 gtaaatagcc gatacagatg aagctaaaga acttccgata tacttaactg cataaagatt
     2581 ggcctgcata ccgatagggc ctgctagcaa ggctccgatg ataacactga cattgcgaat
     2641 atttaagaaa attgagaggc gaactttccc ttcttttacc aagagaaaag ctagtaagat
     2701 aaagatgctc aaaaaatcat gagctgcagc caccacaaag ggtgacaaat ctgtaaaaat
     2761 cgaaaagata taagcactaa ccgttagacc caagccccag aaaatacctg agagaaggcc
     2821 aaaaggaact ccgtttttac ttttcatctg aacctccata agaagccaaa cctttaacag
     2881 ctctttggta acgattcaca ccatagtcac caaaatcttc accttgctct tccttataga
     2941 cagtccatag actccaaata gtatcttgta aaattttata aatagcaatc ttttcatgag
     3001 aaaccggtgt ttggtcactc tcatagtgag ataagaaagt ttcttcctct tgggaagtga
     3061 attcagactc taaaaagagg gcagccaaat cccacattgg atcattcatt gatgaatatt
     3121 cccagtcaat caaataaagt cgtccttgag gtgattcgat aaagttttca ggcaccaaat
     3181 cgatatgaca agattttctg tcaacaccta agtcagccag tcttttctct aaggagaaga
     3241 ctgcatttct aacagattca tagttggcat aaggaatttg ttcttcaatc aaggattcgt
     3301 attttttgat ttcttcaaaa ggagcaaatt ctcctcttaa ttccttagca gacgtatgaa
     3361 tagtttgtaa tattggagca attttgtcga acttggtctt gattgacgtt gaatcaagcg
     3421 taatcgcaga ttcgatatac tcatttactt tgataccagc ttcaatatca aaaagataat
     3481 tttttacatc taagcctaaa tcctttagta gttcaagatt gtacttttca tcttgtcgat
     3541 tgataagctt ttctgtccct ttaccaaaga atttaacaat gtattgctta tttgttgttt
     3601 tggccaaata gttttgattg gtcattccac ccagttgttc aacactgagg acttcctctt
     3661 cttgactaag taaggaagaa attttttctt taatgatttt ctccacaatt aacctccagt
     3721 acttatactt cccacttaaa cactgtttta aaggctgtgt ttaaatcggt tgcaaagaca
     3781 cgatgaatat ctttaatttc tcttacaggt tcttctagat aaaggatatt tttaagacga
     3841 ttggcaaatt tcttgacttc catcatttgg atagcatttt taaaatcaat gcgaccagaa
     3901 cgagatgacc caaccaaaat caagcccttt tctaaggcat cgcgagtatt gagattgact
     3961 ttatattcgc taactcccat catgagaatc gttccctgag gacgaatgta gcgaatcaag
     4021 tcattaatag ctggtccagt accatcacca ccacaacatt caaaagcatg gtcaaaggcc
     4081 aactcttcag gaatattatc cgtaatatag cattctttgg caaatgagaa gagttccaac
     4141 ttttcccaat gacgaccaat aaccacaatc tctgcttctg gcaaagtata gttgataata
     4201 ttggcaacca caaaagctaa acttccatct ccaataacgg cgatccgctc ccgcttgcta
     4261 tgagcaagag tcaatagacg attcatagcg tgcatgccca cactgacaaa ctctgtaatg
     4321 gctgcaaccg tatcttcaat agcatcataa gccaccacac gatctttagg gagagaaaca
     4381 aactctctca taaagccatc aaatccacta gacaagaaat gggtccctgt catgtagttt
     4441 tcatagaatt cttcatcact ctgcatagga gactgattgg gaatcatgac aactttttga
     4501 ccaacctcgt aggttccggt cgggtcagaa atgacggttc cacatgactc gtgaatcatt
     4561 gccattggaa gctttttatt caaaatcttg ggatcacgtt ttccctgata gtaacgctga
     4621 tccgcatgac agacagccat gtagttggga cggataagga tatgattctc ttggtcaata
     4681 gcctcttcct gatatttgac attgataaac ttaggcttag ttagttgata aatttgatta
     4741 atcattttac tagtctttct caatcatact ttttgcaatc ttcaaatctg ttacggttgt
     4801 aatcttcaga tttgagtatt cacctttggc caaggccaca tcttttcctt tgatcacaaa
     4861 gattttacat gcatctgtca agatttcctt ctcttcatca gaaagagatc cataaaggtc
     4921 catgaagtcc ttgcaacgga atgtttgagg tgtttgtcct tgataaaggt gagcacgatt
     4981 tggaatatct gtaataaatt gaccattggt actttcaacg atagtatcaa ccgcttctac
     5041 cactgtgtcc actgcgtcat gattttgggc aagttggata ctgtcctgaa tcatgcgaag
     5101 tgtaataaat ggacgaacag aatcgtgggt aacaacgata tcctctggag taagcggacg
     5161 ataagcatca atggcttcaa tgattttctt aatacttgta ttgcggtcag caccaccctt
     5221 tgtaatgatg atacgttcct tataaagagg aagatattta tctacaagat cttctgcatg
     5281 agaaacccag tctccatgaa caccaactac aattttttca atacttggct ccaagacaaa
     5341 tttttcaatt gtatgaatca aaataggtcg atcacctagc tctaaaaatt gttttggcaa
     5401 gttactgatc cccatgcgtg tgccagttcc accggcaaga attcctgcat aaatcatcta
     5461 ttttctcctt tgtcttacta aaaaaactta ttctctctat tataccacaa tctagtccta
     5521 tcataaaaga aatactggtc ctccccttct agaataggag aattatgcag ttctattatt
     5581 caaagaaact agtccatttc tagtaatgac taggaattct gtagcaatta ctaataaatg
     5641 gctgcgaaat tttagagtcc ttcagaataa tatactttcc ttaaagaaaa cttaaaactt
     5701 aagatttgaa acttcaaagt gattacttga cttactgccc ttcatttctt attagctaac
     5761 tttatgatat aatgaaaaac gaggtaaatt gaatgaaaag tataaaatta aatgctctat
     5821 cttacatggg aattcgtgtc ttgaatatta tttttcccat cctaactgga acctatgtcg
     5881 cgcgtgtctt ggaccgaact gactatggtt acttcaactc agtcgacact attttgtcat
     5941 ttttcttgcc ctttgcaact tatggtgtct ataactacgg tttaagggct atcagtaatg
     6001 tcaaggataa caaaaaagat cttaacagaa ccttttctag tcttttttat ttgtgcatcg
     6061 cttgtacgat tttgaccact gctgtctata tcctagccta tcctctcttc tttactgata
     6121 atccaatcgt caaaaaggtc taccttgtta tggggattca actcattgcc cagatttttt
     6181 caatcgaatg ggtcaatgaa gctctggaaa attacagttt tctcttttac aaaactgcct
     6241 tcatccgtat cctgatgctg gtctctattt tcttatttgt taaaaatgaa cacgatattg
     6301 ttgtctatac acttgtgatg agtttatcga cgctgattaa ctacctgatt agttattttt
     6361 ggattaaaag agacatcaaa cttgttaaga ttcacctaag tgattttaaa ccactctttc
     6421 tccctctgac agccatgtta gtctttgcca atgccaatat gctcttcact tttttagatc
     6481 gcctcttcct cgttaaaaca gggattgatg tcaacgttag ttactatacc atagctcagc
     6541 gaattgtgac cgttatagct ggggttgtaa caggtgcaat tggagtgagt gtgcctcgtc
     6601 tcagttacta tctggggaaa ggagacaaag aagcctatgt ttctctggtt aatagaggta
     6661 gtcgaatctt taacttcttt atcattccac tgagttttgg actcatggtt ttaggaccaa
     6721 atgccatcct actttacggt agtgaaaaat atatcggagg cgacatcttg acctctctct
     6781 tcgcttttcg tacgattatc ctggccttag ataccattct tggttcccaa attctcttta
     6841 ccaatggcta tgaaaaacgt atcacagtct atacagtctt tgctgggcta ctcaatttgg
     6901 gcttgaatag tctcctcttt ttcaaccata tcgtggctcc cgaatactac ttactgacaa
     6961 ctatgttatc agaggcttct ctacttgttt tctatatcat tttcatccat agaaaacaac
     7021 tcatccactt gggacatatc tttagctata ctgttcgata ctctctcttt tcactttcct
     7081 ttgtagcaat ttatttcctg attaatttcg tgtatcctgt ggatatggtc attaatttgc
     7141 catttttgat taatactggt ttgattgtct tgctatcagc tatctcttat attagtctac
     7201 ttgtcttcac aaaagatagc attttctatg aatttttaaa ccatgtccta gccttaaaaa
     7261 ataaatttaa aaaatcttag gagtttaaaa tgaaacaact aaccgttgaa gatgccaaac
     7321 aaattgaatt agaaattttg aattatattg atactctctg taaaaagcac aatatcaact
     7381 atattattaa ctacggtact ctgattgggg cggttcgaca tgagggcttt atcccttggg
     7441 acgacgatat tgatttatcc atgcctagag aagactacca acgatttatt aacatttttc
     7501 aaaaggaaaa aagcaagtat aagctcctat ccttagaaac tgataagaac tactttaaca
     7561 actttatcaa gataaccgac agtacgacta aaattattga tactcgaaat acaaaaacct
     7621 atgagtctgg tatctttatc gatattttcc ctatggatcg ctttgatgat cctaaggtca
     7681 ttgatacttg ttataaacta gaaagcttca aactgctgtc tttcagtaaa cataaaaata
     7741 ttgtctataa ggatagcctt ttaaaagatt ggatacgaac agccttttgg ttgctccttc
     7801 gactggtttc tcctcgttat tttgcaaata aaatcgagaa agaaattcaa aaatatagtc
     7861 gtgaaaatgg gcagtatatg gcttttattc cttcaaaatt taaggaaaag gaagtcttcc
     7921 caagtgctac ctttgataaa acaatcgatt taccctttga gaatttaagc cttcctgcac
     7981 ctgaaaaatt tgatactatt ttgacacaat tttatggaga ttatatgacc ctaccaccag
     8041 aagaaaaacg cttctacagt catgaatttc acgcttataa attggaggat taggatgcaa
     8101 tatttagaaa aaaaagaaat taaagaaatt caactagccc tgctggatta tattgatgag
     8161 acttgtaaga aacatgatat tccttatttt ctcagttatg gaaccatgct tggagccatc
     8221 cgccacaaag gtatgattcc ttgggatgat gatattgata tttcccttta tcgtgaggat
     8281 tatgagcgtt tactgaagat tattgaagaa gaaaatcacc ctcgctacaa ggttctttcc
     8341 tacgatacat cttcttggta cttccataat ttcgcatcga ttttggatac ttctactgtt
     8401 atagaagacc atgttaagta caagcgtcat gataccagcc ttttcatcga tgtcttccca
     8461 attgatcaat ttacagactt gagcattgtc gacaagagct ataagtatgt ggcccttcgt
     8521 caactagctt acatcaaaaa atcacgagca gttcacggtg atagcaaact aaaagatttt
     8581 cttagattat gtagctggta cgctctccga tttgtcaatc ctcgctactt ttacaagaaa
     8641 attgatcaac tagtcaaaaa tgctgtaacc aacattcctc aatatgaagg aggagttggg
     8701 atcggtaagg aagggatgaa agaaatcttc ccagttgata cctttaaaga actgatttta
     8761 actgagtttg agggccgtat gttgcctgtt cccaaaaaat atgaccaatt tttaacccag
     8821 atgtatggcg attatatgac accaccatca aaagaaatgc aagagtggta tagtcatagc
     8881 attaaagctt atcgcaaaaa ctgattgagg gggattatac aaactactaa gatagaggtt
     8941 attcaaaaac ataattttag tagaaaatga aatacatatt cccacaataa aacgcatcat
     9001 atcaaggttt ttgaaaaacc ttgatatgat gcgttttata attttaaaga cttttttcta
     9061 tagtagattg aaataagatg cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa
     9121 tattttagta ttcctgtgta ctgttctaaa ttcagtctgc tatatcttat ttttctattt
     9181 aaatcgcttc tgtaacaaag ctacgacttt caagtacctt aagcatggca ttagctgtat
     9241 ctagcgctgt gaagaggggc accccgtgtt caatggctga acgacgaatt tgctcaccat
     9301 cttcgtcagc agttcgtttt gttcctactg tgttaatgat agcttgaatt cttcctttgc
     9361 gtacaaaact tgggatatcc ttatcgtcat caccaatctt accaacaggt tgggcttgca
     9421 agccatggct agcaaagaag gctgctgtcc cttctgtcgc aaggattcca taaccaatgt
     9481 tttggaaacg acgagccaag ttcaaggctt cttctttggc atcatcagcg atggtaaaga
     9541 cgacattacc aaaagttggc aagtgtagat aagaagcttc aaaggcttta tagagagctt
     9601 tttccaaagt agcatcagaa cccataactt cacctgttga cttcatttca ggaccgagca
     9661 agctgtctac cttagctagt ttggtaaagg agaagacagg tgccttgata tgaacacggg
     9721 tgctttcagg gtaaagtcca ttttggtagc caagttctga taaactttga ccaagaatga
     9781 gtttggtcgc tacttgagcc ataggaatat tggttacctt agataggaat ggaacagtac
     9841 ggctggcacg tggattgacc tcaataacgt agactttttc atccttgata acaaactgga
     9901 tgttcatcat tccaaggcag tgaagaccga ttgctaagcg tttggtgtag tctgcgatgg
     9961 tctcctgaac cttttgcgac aaggtttgtg gtgggtaaac agccattgag tcacctgagt
    10021 ggacaccagc acgttcgata tgctccatga taccaggaat gagtacattt ttaccatctg
    10081 aaatggcatc aacttcgcac tcttgcccaa cgatataaga gtcgacaaga actgggtggt
    10141 ctggactagc cttaacagca gttcgcatgt aagaacgaag gtcttcttcg ttttcaacga
    10201 tttccatggc acgtccacca agtacataag atgggcggac aagaactggg aagccaatct
    10261 tgcgagctgc aagagctgct tcttcttcat tggtagctgt ttgtcctggt ggctgtggaa
    10321 tatccaattc tttgagagct tgctcgaaga ggtcacggtc ttcggcacga tctaggtcag
    10381 caacctgtgt accaaggatg gtcacacctg cttttgccaa tggctccgca aggttgatgg
    10441 ctgtttgacc accgaactga acgataactc cctttggttg ttccaagtca atgacgttca
    10501 taacatcttc gaatgtcaat ggctcaaagt aaagcttatc tgatacagag aagtctgttg
    10561 aaacggtctc tgggtttgag ttcatgatga tagcttcata accagctgcc tggatagcct
    10621 taacagagtg aacggttgcg tagtcaaact caaccccttg accaatacgg attggacctg
    10681 aacctaggac aagtacagat tctttatcag atctgataga ttcattttcc caaccatagg
    10741 ttgaatagaa atatggcgtt tcggagtcga actctgccgc acaagtgtct accatcttat
    10801 aaactggaac aatcttgttt tccaagcgaa gttggcgaac ttgatcatca gtcgttcccc
    10861 agagttcagc aatcttacgg tctgaaaaac cattaagttt ggctgttttc aaaacttcta
    10921 gatcttgtgg atgagcaccc aattcttgct caatttcaaa gatatgcaag agtttatcaa
    10981 gatagaagat atcaattttt gtaagctctg caatttcttc aggtgtgtag ccacgacgaa
    11041 ttgcttctga tacgtagaag agacggtcat cttgggcttt gacaaccttt tcaatcaagg
    11101 catcatcaga aactgctgca agttcaggta tttcattgtg gtgcacccca atttcaaggg
    11161 agcggcaggc cttgagaaga gattcctcga tgttacgacc gattgccatg acttctccag
    11221 tcgccttcat ttgtgtaccg agacggcgtt cacccttttc aaacttgtca aatgggaaac
    11281 gtggaatctt agcaactacg tagtcaaggg ctggttcaaa catggcatag gttgaacctg
    11341 taactgggtt tataacctca tccaaggtca aacctactgc aatcttggca gccaacttag
    11401 caatcggata tcctgtcgct ttagaagcaa gggctgacga acgtgataca cgagggttta
    11461 cttcgataac ataatacttg aagctgttag gatcaagagc tagctgaaca ttacatccac
    11521 cttcaatctt gagggcacga ataatgctca agctcgcatc acgaagcatt tggttttcat
    11581 agtctgacat ggtttgcgca ggggcaaata caatggaatc ccccgtgtga atcccaactg
    11641 ggtcaaagtt ttccatgtta caaacaacca aggcattgtc agctgagtca cgcatcactt
    11701 cgtattcaat ttccttgaaa ccggcaatcg aacgctcaat caaacattgg gtaacaggtg
    11761 acaatttcaa accattttca gtgatttcac gcaattcttt ctcgttggca cacataccac
    11821 caccagtacc accgagggta aaggctggac gaacgatgac tgggtagcca attgtcgctg
    11881 caaaggcaac tgcttcttct actgtgttaa caatttcaga ttctggaatg ggttgttcaa
    11941 gctcttccat caattgttta aagaggtcac ggtcctccgc ttggtcaatg gcagataatt
    12001 tggtacccag aagttcaacg ccaagctcat ctaagatacc atttttagac aattccatgg
    12061 ccatgttgag acctgtctga ccaccgagtg ttggtagcaa ggcatctgga cgttccttac
    12121 gaagaatacg tgtcacaaac tcaagtgtaa tcggttcaat gtaaaccttg tcagcaattt
    12181 ccttgtccgt catgatggtt gcaggatttg agttaaccaa aacaacctca taaccttcct
    12241 ctttcaacga caagcaagcc tgagtcccag cgtagtcaaa ctcagcagcc tgaccaataa
    12301 taatcggacc agaaccaatc accataattt tttgaatatc agtacgttta ggcatagttt
    12361 atgatacaaa gcccgtaaag aacacagtga aaataggaaa ctcggcgcag acgccttcag
    12421 cgtcaagacg atgtttatct ttttcacaca gttcttaggg cgtgttcact tccgcgaaca
    12481 atgtatcttc tcctttctat tcgtcgcctc tcagggcgac attaaataag atacaaagga
    12541 cgactagaaa gcgattgaat tttaggaaat caaggaagga ttgacaatcc aagttggttt
    12601 ctctaaattc tgagctttcc gtccatgttc agttacataa attctccgac gagcttttac
    12661 tcattcttag tttgattgtt taaaaacttc catcatctcg ataaactcgt caaataggta
    12721 gctagcgtcg tgtggcccag gagctgcatc tgggtggtat tgaacagaga aagcaggttg
    12781 gtatctgtgg cgcacacctt ccactgactt gtcattgatt tcttcgtggg taataatcaa
    12841 gtgctctggc aaatcctcgc ggctgactgc ataaccatgg ttctggctgg tgaagtctac
    12901 tcgtcctgtt gcgatttcac gtaccgcatg gttgaatcca cggtggccaa acttcatctt
    12961 ataggtctta gccccgtttg ccattgcaaa gagttggtgt cccatacaaa taccaaagat
    13021 tggaattttt ccttgtacac cgcgaatcat gtcgagtgct tgtggaacgt cttctgggtt
    13081 acctggacca tttgacaaca taactccatc aggattgaga tggagaattt cttcagccgt
    13141 tgtcgaataa ggaacaactg tcacgttaca gttgcgttta gaaagttcac gtaggattga
    13201 gtgcttgaga ccaaagtcca ctagcaccac gctcaaacca actcctggag ctggataaga
    13261 cgttttagta gaaacctgtt tgatattgtc tgtcggtaaa actgttgctt ggagctggtc
    13321 cgtcacatgg tccatactgt ccccaacatg ggtcaaggtt gcacgcatag taccatgctt
    13381 acggataatc ttggtaagag cacgcgtatc aattcctgaa atccctggaa ttttcttggc
    13441 tttcaaaaat tcatccaagg tcatttggtt gcgccagttg ctagctctac gcgcttcttc
    13501 aaaaacaacg actcccttac aagttggaat aatggattca taatcatcac gattaatacc
    13561 ataatttcct accaaaggat aagtaaaggt caagatttgt ccattataag actggtctgt
    13621 aatggattct tggtagccgg tcatccctgt attaaagacg atttcgcctg ttacatcaat
    13681 atctgctccg aaggccttgc cttcaaaaac tgtgccatct tctaatacta gaattctttt
    13741 tgtcatattt tcacctctcg tggacgctca ctggcgtctt ttaacgtctt gtgttttagt
    13801 tggcgtttct actcgctagt acggattcta agattgccat tcgaacaaag acaccattgg
    13861 tcatttgttg gacaatccgt gattttggtg cttcaaccaa gtggtctgct atttctacat
    13921 cacgattgat tggagctggg tgcatgagga ttgctgtttc tttcaaacga tcgtaacgtt
    13981 cttgagtcaa gccatgttgg gcatgatagt cttcttttga aaatacagct ccactatcat
    14041 ggcgttcgtg ttgcacacgg agaaacatca tgacatcaac ctgatcaatg atttcatcaa
    14101 tggttacaaa ctttccatag tctgcaaact cttgacttct ccattcctca ggtccagcga
    14161 aaaagagttc agctcccaag cgtttcaaaa tctgcatatt ggatttggca acgcgtgagt
    14221 ggtccaagtc acctgcaata gcaactttaa gaccctcaaa gtggccaaat tcctcataaa
    14281 tggtcatcaa atcaagcaag ctctggctag ggtgttggcc cgaaccatct ccaccattga
    14341 tgatggaagt cgtaatcgtt ggactagcaa tcaattctct atagtagtcg acctctggat
    14401 ggcgaatcac acagacatcc actcctaaag cagacagagt caaaatggtg tcataaagtg
    14461 tctcaccctt attaaccgaa ctagtcttca catcaaagtc aagtcgttcc aatccaagtt
    14521 taatctctgc gacttcaaag gacttatgtg tccgtgtaga atcctcaaag aagagattgg
    14581 aaacaatcgg atggtcttca tagggaagct gggctccatt tttaaactca attcctcgct
    14641 tgatcaattt cattacttga tcgacagtga ggtcttccat ggacaccaca tggttcaatg
    14701 cttgttgatt ttctgacatg gctactcctt taactttcta agcttcttca gtaatcagaa
    14761 ctctgtcttg gtcatcaagt tctgtcatct ctacgatgat ttcttcagaa cgactggttg
    14821 ggatattttt tccaacgtaa tctggacgga ttggcaattc tctatgtcca cgatcgacta
    14881 gaactgctaa actcacacgc gcaggacgac catgaccgac aatattatca atagcagcac
    14941 ggatggtacg acctgtatag agcacatcat ccaccaagat aacttcgcgg tctgtcacat
    15001 cgacagaaac caaagaagta tcttctccac ttttaacatc atcacggaaa ggtttagtat
    15061 ccaattccac aacaggaact gaaagatttt ctaactgctt caaacgttct tggattcggt
    15121 gggcaataaa gacaccacga gttttaatac cagccaagac gatcttattc aaatctttgt
    15181 tgcgttcgat aatctcataa gtaatacgcg taatcgctcg tttgacggtc aattcgtcta
    15241 caacttcttt tgttttcatg acaaacctcc aaaaagaaaa gtctccttaa acaaggagac
    15301 ttgaaattta tagccaagcg agccctactg caaacagtat agacttcacc cttctacttt
    15361 atcgcgctcc ttgcctgcct cacgggacag gtttaaagga atatttagtt atcatttact
    15421 atagcacaaa gcatgcttaa aatcaagcaa aaagtttcaa tgtagcatct tacaaattgc
    15481 taaaatcata taattgtggg tactggtcac actctggatt ttttggatgg caaatggctc
    15541 ttccaaaata aatcatggcc tgatgggcag ctaaccactg ctcaggcggc aagatatcca
    15601 tgacccgctt ttccacctca agtggcgtcg ctgatttttt gacaatatcg tggtgtttgc
    15661 aaatacgctc cacatgagta tccactgcaa aggctggaat cccaaatcct acactcatga
    15721 caacattggc tgtcttgcga ccaacacctg ccaaactctc caattcttca cgtgtctgag
    15781 ggacttgacc atcaaaatcg tctagtaact gttgggcaca ttttttaagg aatttagctt
    15841 tattccgata caatcccaag cgagaaatat gtgaagcaat ctcactctct gtcgctacag
    15901 acatagcttg gggtgttgga aaggcaacaa agagacctgg tgtggcctta tttaccgctg
    15961 catctgtcgt ctgggctgat aacatgaccg caaccaggag ttcaaaatga ttggtaaaat
    16021 caagactagg cttggcatct gggaagaggg caatgatttc ttctagcacc tttcgtgctc
    16081 gtttttttga caagaccatt attcatctcc gtcaaatagt ccttgtaagc cagcaaaagg
    16141 actgttttct tctttcttta ctgcttgttg agcttggtat tcttcctctg tcatgatttg
    16201 ccagtcattt cctgagataa atccttgacc agcttcttct tcagccgtca agaccttgat
    16261 aggaatgttt agcaggatat tgtctgatac actctcagca aggtcaagct ccccattttc
    16321 aatgggcaag accaagtcat catctaaaac ttcttgatct agctggttag ttgcgccttc
    16381 catgaaaact tccgtgactg gataagattc aactaactca actggctcca tactgcgact
    16441 cgaagcaaga acaatggtat aagatagttg ataatctaag aaatacatac ggtcttcata
    16501 ttgtactttc ccaactgcaa ggatatcttt tacatctaaa atttcttgat tacgtgcacg
    16561 caggtcatca actaaatcta acgtttgttc aaagttcaaa ccttcagact gcttacgaat
    16621 ttcttgaata tttaatttca tacttcctcc ataaagattt actctcttga ttataccatg
    16681 aaaaggctac aaatcagcac accaaacttt gtaattaaaa ttcaaaattt taacatattt
    16741 actatgacag ttttattttt tagtgctata ctatagggaa agagtacatc agatcaagga
    16801 ggatgctcat atggaagaca tggaagacaa gaaactcatt caactcctat ccaagttaaa
    16861 taaaagctac caaaactgta aacagggtac ggcagatgat attcgactac aagagctgct
    16921 aaacactact atgcaagagc tcaaaaaaac ggaaaagttg aacaacagta tcttaattga
    16981 tcttgagaaa ttttaccaac ccaccagtcc tctgattgga ctgggtagcc taaaactaaa
    17041 cgatcaagca cgcactgctt ggcgaaacta tgataaaatc cattacgagc atgtcaaaca
    17101 cgtactaagt ctctatggac ctgtttttgg attttagagc atagaatttc cagttttctg
    17161 ttgacaaaat ttccttaaaa gtatagtata aagatactaa tactcggagg taagggagac
    17221 atgaacaact aagtctatca aataaagaac ctttatttag tagatcttgt ttttgtctct
    17281 ttttgtgtgc tcttttatgc tctttttctg gcatgttaat agagtttttt tgacatagac
    17341 tttgggctct actaggtaaa gtagagcttt ttgttatgca ctatcgacat tctagaaagg
    17401 gcaatagtat gataaaaatc aatcacctga ccatcacaca aaacaaagat ttacgagacc
    17461 ttgtctctga cctaagcatg accatacaag acggggaaaa ggtagctatt attggcgaag
    17521 aaggaaatgg taaatcaacc ttacttaaaa ctttaatggg ggaagatttg tctgatttca
    17581 ctatcaaggg agacatccag tctgattatc agtcactggc ctacattcct caaaaagtcc
    17641 ctgaggacct aaaaaagaaa actttacacg actacttctt tttagattct attgatttag
    17701 actacagtat cctctatcgt ttggcggagg aattgcattt tgatagcaat cgtttcgcaa
    17761 gtgaccaaga gattggcaat ctatcagggg gcgaagcttt gaaaattcag cttatccatg
    17821 agttagccaa accctttgag attctatttt tagatgaacc ttcaaatgac ctagaccttg
    17881 agacagttga ttggctaaaa ggccagattc aaaagaccag gcaaaccgtt attttcattt
    17941 cccatgatga agactttctt tctgaaacgg cagacactat tgttcacttg cgactggtca
    18001 aacaccgtaa agaagcggaa acgctagtag agcatttaga ctatgatagc tatagtgagc
    18061 agagaaaggc taattttgcc aaacaaagtc agcaagctgc taacaaccaa agagcctacg
    18121 ataaaaccat ggaaaaacat cggagagtta agcaaaatgt agaaactgcg cttcgagcta
    18181 ccaaagatag tactgccggt cgcctattgg ctaaaaagat gaaaactgtc ctctcacaag
    18241 aaaaacgcta cgaaaagaca gctcagtcca tgactcaaaa gccacttgaa gaggaacaaa
    18301 tccaactttt cttttcagac atccaaccat taccagcttc taaagtctta gtccaactgg
    18361 aaaaagaaaa tttgtccatt gacgaccgtg ttttggctca ggaactgcaa ctaactgtcc
    18421 gtggccaaga aaaaatcggt atcatcggcc caaatggtgt tgggaaatca actctgttag
    18481 ccaaattaca gcaacttctg aatgataaaa gagagatttc acttggtttt atgccacaag
    18541 attaccacaa aaaactgcaa ttggatttat cctcaatagc ttatatcagc aaaactgggg
    18601 aaaaagagga acttcagaaa atccaatctc acctagccag tctcaatttc agttatccag
    18661 aaatgcagca tcaaattcgc tccttatctg gcggacaaca gggaaaactc ctgcttttgg
    18721 atctagttct gcgcaaacca aactttctcc tgctggatga acccacacga aacttttctc
    18781 ccacttctca accccaaatc agaaaactct ttgcaaccta tccaggcggt ctcatcactg
    18841 tttcgcatga ccgtcgtttc ttaaaagaag tctgctcgat catctatcgc atgacagaac
    18901 acggtttgaa gctagttaat ttagaagatt tataaatttg caacatagca aaaatccaga
    18961 gacgacctct ggattctttt acatctgttt taaacgttca atccgttctg agataggtgg
    19021 gtgggtataa aagagttttt ggaacccccc acctttctta ggatcattga tataaagggc
    19081 actgctagca tcatcgacgt ggcgactcat aggtttgcta ttgtccaact tatctagggc
    19141 attaatcatt ccctggggat tgcgagtcag ctcgacacta gatgcatctg ccagaaattc
    19201 cctctgacga gaaatagcga gctgaaccaa ggttgcagcg agaggtgcca gtacaatagc
    19261 tagtagggaa accactagca taatgatttc aagaccattt ccatctcggt catcatcact
    19321 tcgtctgcga cctgctccac cccaccacat catacgacct gccatactag aaagcatggt
    19381 gatagcacta gcaagggcaa ctgcaatagt cgaaatacgg atatcataat tacgaatatg
    19441 actgacttca tgtcccataa cagcttctag ttcttcacga ttcatgatag ctagtagacc
    19501 tgaagtcgca gcaacagccg cattttgagg attagaacct gtcgcaaagg catttaaggc
    19561 tggatcatca atgatgaaaa cacggggcat aggaatctga gcgaccagag ccatatcttc
    19621 cactacatgg tagaggtctg gtgccgtttg ctcatccacc tcacgcgctc cattcatgga
    19681 catgacaatc tctgtcgatt gaaaaatcat agacaaagcg tagataaagc cgataatcag
    19741 tgcgataacc aaaccaccaa gtccagatct tataaagaga taaccaaccg cataaccaac
    19801 aagagctaag agtaggaaaa ataccagcaa caaaatccag gtttttcgtt tattgcttgc
    19861 aatttgatca aacaacatct tagtcaccta aaccgctaaa atcaacttta ggaaccgact
    19921 tttcctcttc agatgtttga aggaaatctg ccgctttaaa tccaaacatt ccagcgataa
    19981 tattgctcgg gaaagtttct aattttacat tgtagttgct gacaacactg ttatagagtt
    20041 gacgagagta agaaatttta ttttctgtgt ttgtcaactc ctcttgcaat ttaacaaagt
    20101 tagcactagc tttcaaatct ggatagcttt ctgcaactgc aaaaatacct gaaacctgac
    20161 gagtgagggc atcactggct ttcatagctt ctgctggtga agtcgctgcc gccacttggt
    20221 tacgtagttc tgccaccttt tcaagggtag aaccttcata tttggcataa ccttttacag
    20281 tctcaatcaa gtttggcaag aggtcatttc gacgtttcaa ctgaacatca atctgactcc
    20341 aagcctcctt ggtttgcata cgatttttaa ccaaaccgtt atagctaaca atcacaaaaa
    20401 taacaataag agcgataact ccaagaataa tccaagtcat aatataagtc ctttctgctt
    20461 ttagattagt accagtatat caaattttct atgattgtgg taaaataaga tgatactaaa
    20521 gaaggaaata actatgaaac caaaaacatt ttacaacttg cttgccgagc agaatcttcc
    20581 actttcggac cagcaaaaag aacaatttga acgttatttt gagctcttgg tcgagtggaa
    20641 tgagaagatt aatttgacgg cgattacgga caaggaagaa gtttatctca aacattttta
    20701 cgattcgatt gcacccattc ttcaaggttt gattcccaat gaaactatca aacttcttga
    20761 tatcggggct ggggcaggat ttcctagtct accaatgaaa attctctatc cggagttaga
    20821 tgtgaccatt attgattcac tcaataagcg catcaacttc ctacaactct tggctcaaga
    20881 actggatttg aacggagttc atttctacca cggacgtgcc gaagattttg cccaagacaa
    20941 gaacttccgt gctcaatatg attttgtaac agctcgtgcg gttgcccgta tgcaggtcct
    21001 atctgaattg accattccct accttaaggt tggtggaaaa ctattggcac tcaaggccag
    21061 caatgcgcct gaggaattat tagaagctaa gaatgccctc aatctccttt ttagtaaggt
    21121 cgaagacaat ctcagctacg ccctaccgaa tagagatccg cgctatatca cagtggtaga
    21181 aaagaaaaaa gaaacaccaa ataaatatcc gcgtaaggct ggtatgccaa ataaacgccc
    21241 actttaaatt ttttagtaaa caaatgttta caaaatcagc ctcgctcttt tatttctagg
    21301 ctcgggaaaa aatgatttac aaaatcattt ttttctgcta tactatccta agcaaaggtt
    21361 tttaatgtca tcccgtgagg tgacgaagac gcagaaatat ttaaaactct ttaaaatcta
    21421 aattttaaag aagtcttact ctgagggcct attgctgtaa aataatgggc tcttttttga
    21481 tgcccaaaag tgaggtttat atgaaacaag aatcaactgt tgatttgtta ctagacgttg
    21541 accaacgtcc ttcggctgga aaaggaattc tccttagttt ccaacacgtt ttcgccatgt
    21601 ttggtgcgac catcttggta ccattgattt tgggaatgcc tgtatctgtt gccctttttg
    21661 cttcaggtgt tggaacactc atctacatga ttgctactgg ttttaaagtt ccagtttatc
    21721 taggttcttc atttgccttt atcacagcta tgtcactggc tatgaaagaa atgggggggg
    21781 atgtatctgc tgcccaaaca ggggttatct tgactggttt ggtctatgtc cttgttgcta
    21841 ccagcatccg atttgtagga acaaaatgga ttgataaact cttgccacca atcattatcg
    21901 gtcctatgat catcgttatc ggtcttggac ttgcaggttc agctgttacc aatgcaggtc
    21961 ttgtagcaga cggaaattgg aaaaatgctc tggtagccgt tgttactttc ctaattgctg
    22021 cctttatcaa tacaaaagga aaaggcttcc tacgaatcat tccattcctc tttgccatta
    22081 tcggtggtta ccttttcgca ctaactcttg gcttggttga ctttacacca gttcttaaag
    22141 ccaactggtt cgaaattcct ggtttctact tgccatttag cacaggtggt gcctttaaag
    22201 agtacaatct ttactttggt ccagaagcca tcgctatctt gccaatcgct atcgtaacaa
    22261 tttctgaaca tatcggagac catactgttt tgggtcaaat ctgtggtcgt caattcttaa
    22321 aagaaccagg tcttcaccgt actcttcttg gtgacggtat cgcaacttct gtttctgcct
    22381 tccttggtgg accagccaat acaacttacg gagaaaatac aggggttatc ggtatgactc
    22441 gtatcgcttc tgtctcagtt atccgtaacg ctgccttcat cgcgattgcc ctcagcttcc
    22501 ttggtaaatt cactgccttg atttcaacta ttccaaacgc tgtacttggt ggtatgtcaa
    22561 tccttctcta tggggttatc gccagcaatg gtttgaaagt cttgattaaa gaacgtgttg
    22621 atttcgctca aatgcgaaac ctcatcatcg caagtgctat gttggttctt ggacttggag
    22681 gagctatcct taaacttggt ccagttacac tttcaggtac tgccctttca gccatgacag
    22741 gaatcatctt gaacttgatc ttgccatacg aaaataaaga ctaagagtct aaatacacct
    22801 aatccactca gacagctgag tggatttttc gtataccata ataaaagtgt cttaacaaaa
    22861 ttattaaaat caaaaaacgt ataatatcag atattctaaa accttgatac tgtacgtttt
    22921 atcatagaaa tttttacttt attttctcat caaatgagat ttgcatcaat ctcttgtctt
    22981 acttgcgttt cttcttcgct ttcttcattt tgttagccat acgtttcatg gactgtttca
    23041 tggcaaattc accaatttta cctttcaaac cgccaccaaa catctggctc atatctggca
    23101 ttcctgctcc tccgagagct gataagtcag gcataccgcc ttgtcccatc attccttcaa
    23161 gggcagacat atccattcct cccatatttg gcatattttt aggaaggtta tttggattaa
    23221 tccccatttg cttcatcatt ttattcatat ccccagacat aacaccctgc atgagctgtt
    23281 tagcctggtt aaagtccttg atgaatttat tgacttcgac gaatgtattt ccagaaccag
    23341 cagcaatacg acggcgacgg cttggattta acaaatctgg gttttcacgc tcttcaggtg
    23401 tcatcgaaga cacaatggca cgtttacgag caatctggcg ttcatccacc ttcatgtttt
    23461 gaagggctgg attgttggcc atacctggaa tcatcttgag caagtcttcc atcggcccca
    23521 tattttgcac ctgatctaat tgatcgatga aatcattaaa atcaaaggtg ttttcgcgca
    23581 tcttctcagc catttcaagg gctttttgtt catcgtattc ctgagaagct ttctcaatca
    23641 aagtgagcat atcccccata ccaagtatac ggctagacat gcggtctggg tggaaggttt
    23701 caatgtccgt aatcttttca cctgtaccag tgaacttgat tggttttcca gtaatgtgac
    23761 gaacagacag agcagcacca ccacgagtat cgccatcaat cttggtaagg atgaccccag
    23821 tcacttccaa ctgagcatta aactcacgcg caacattggc tgcttcctga ccaatcatag
    23881 catcaacgac aagcaagatt tcatttggtt gagccaatgc tttcacatca cgaagctcat
    23941 tcatgaggag ctcatcaatc tgcaaacgac ccgcagtatc aatcaagaca tagtcgttat
    24001 gattagtttg ggcttgctcc aaaccttgac gtacaatctc aacagctggt acttctgttc
    24061 caagtgcaaa gacaggcaca tcaatctgtt gtcccaaggt cttaagctgg tcaatggcag
    24121 ctggacgata aatatccgcc gcaatcatca aaggacgagc attttcttct ttcttgagtt
    24181 tgttggccaa tttaccagca aaggttgttt taccagcccc ttgtaaacca accatcatga
    24241 tgatggttgg aatcttaggt gacttgataa tttctgccgt atcagaacct aaaacggctg
    24301 tcagttcctc atcaacgatt ttaataatct gttgcgcagg attaagtgta tcaatgacct
    24361 catgcccgac tgcacgctca cgaactttct tgataaagtc ctttacaaca ggcaaggcaa
    24421 cgtcggcctc gagcaaggcc aagcgaattt ctttggttgc ctcttggaca tcagattcag
    24481 agatttttcc ttttttacgt agatttttaa agacgttctg caaacgttct gttaaacttt
    24541 caaatgccat ttttcttcct cttattctct attatcaatg cttgttaaaa tttctatctg
    24601 ctcctgcaga aagtcatcct tgggatagcg ctccaaaatc tgatcaaaaa tctgactgcg
    24661 gacaatatag tccgagtaca tgtgcaattt catctcataa tcttccagaa tcttttctgt
    24721 tcgcttgata ttgtcataga cagcctgacg actgacacca aactcctcgg caatttcagc
    24781 aaggctgtaa tcatcagcgt agtagagctc gatataattc atttgcttat ctgtcaaaag
    24841 cgccgcataa aattcaaaga gcgcattcat acgattggtt ttttcgattt ccataacttt
    24901 tattatacca aaaattagcc taatctacca cactaggaag ccgatccaag aagatagata
    24961 gctaaatttg aaaaagacat gatcctagcc ccaagtaatt tccaattgat agctggcaaa
    25021 aggatgtccc tcttgatttt gtagttgata atctagttca atcttttgcc tatcaacttg
    25081 ataatggctc gtttggatga taaactcctg catgcccata ggtgtaggaa tataggctaa
    25141 actatcgcta tcctttagaa agcgcataat ggtcttggga ttagaaaatc ggctcatcat
    25201 aagttcttga ccatgaaatt taatcactac tttttccttt tcctcattat agaaaagcag
    25261 gtagctataa tctccttttt catgcacttc cacatcataa agctggtcaa tcacttccaa
    25321 ctgctcatca aactgaattg tatttcgcat ccgaatcttc acatcaggcc ctctttcttg
    25381 tctcttgtcc tactatttta ccaaaaagag caggattttg ctataatggt catatgaacg
    25441 aaaaagtatt ccgtgaccca gttcacaact acatccatgt caataatcaa atcatctatg
    25501 acttgattaa tacaaaagaa tttcagcgtt tgcgccggat caaacaactg ggaacttcca
    25561 gttatacctt ccacggtgga gaacacagtc gcttctctca ctgtctagga gtctatgaaa
    25621 ttgcacgacg catcacagag attttcgaag aaaaatatcc tgaggaatgg aatcctgccg
    25681 agtctctctt gaccgtgacc gctgctctcc tacacgacct tgggcatggt gcctactccc
    25741 atacttttga aaatctcttt gatacagacc atgaagccat tactcaggag attattcaaa
    25801 atcctgagac agagattcac caagtcctgc tacaagtggc acctgatttc ccagaaaagg
    25861 tggccagtgt cattgaccat acctatccta ataagcaggt cgtgcagctc atttctagtc
    25921 agattgacgc agatcgcatg gactatctct tgcgcgactc ctattttaca ggagcatcct
    25981 atggggaatt tgacctgact cgcatcctcc gagtcattcg tcctgtcgaa aatggtatcg
    26041 cctttcagcg caatggcatg cacgccatcg aagactacgt cctcagtcgc taccagatgt
    26101 acatgcaggt ttatttccac ccagcaacac gcgccatgga agttctccta cagaatcttc
    26161 tcaagcgcgc caaggaactc taccctgagg acaagaattt ctttgcacga acttccccac
    26221 atctcctgcc tttcttcgaa aaaaatgtga ccttgactga ctatctggct ctggatgatg
    26281 gcgtgatgaa tacctacttc cagctttgga tgactagtcc tgacaaaatt cttgcagatt
    26341 tatctcaacg ctttgtcaac cgcaaggtct ttaaatccat taccttttca caagaggacc
    26401 aagatcaact tactagcatg agaaaattgg ttgaggacat cggctttgat cccgactact
    26461 atactgccat tcataagaac tttgacctcc cttatgatat ctatcgtccc gaatctcaaa
    26521 atccacggac acagattgag attttacaaa aaaatggaga actggccgaa ctctctagcc
    26581 tgtctcctat cgtccaatcc cttgctggca gtcgccacgg agataatcgt ttctatttcc
    26641 ctaaagaaat gttagaccaa aacagcatct tcgcaagcat tacccagcaa tttttacact
    26701 tgattgagaa cgatcatttt accccaaata aaaactagaa gaggaatttt atgagtatta
    26761 aactaattgc cgtcgatatt gacggaaccc tagtcaacag ccaaaaggaa atcactcctg
    26821 aagtcttttc tgccatccaa gatgccaaag aagctggtgt caaagtcgtg attgcaactg
    26881 gccgccccat cgcaggtgtt gccaaacttc tggacgactt gcagttgaga gaccaaggtg
    26941 actatgtggt gaccttcaat ggtgcccttg tccaagaaac tgctactggc catgagatta
    27001 tcagcgaatc cttgacctat gaggattatc tggatatgga attcctcagt cgcaagctcg
    27061 gtgtccacat gcatgccatt accaaggacg gtatctatac tgcaaatcgc aatatcggaa
    27121 aatacactgt acacgaatca accctcgtca gcatgcctat cttctaccgt acccctgaag
    27181 aaatggctgg caaagaaatt gttaaatgta tgtttatcga tgaaccagaa attctcgatg
    27241 ctgcgattga aaaaattcca gcagaatttt acgagcgcta ctccatcaac aaatctgctc
    27301 ctttctacct cgaactcctt aaaaagaatg tagacaaggg ttcagccatt actcacttgg
    27361 ctgaaaaact cggattgacc aaagatgaaa ccatggcaat cggtgatgaa gaaaatgacc
    27421 gtcccatgct ggaagtcgtt ggaaaccccg ttgtcatgga aaatggaaat ccagaaatca
    27481 aaaaaatcgc caaatacatc accaaaacaa atgacgaatc cggcgttgcc catgccatcc
    27541 gaacatgggt actgtaaaag tatcattttt caataagaat tgattagcaa taaaatccaa
    27601 tgaatttttt tagcaaacta tttaatttaa aacaaaataa tcataataga gacacaaatt
    27661 ctgattgtaa caatttttac ctaaacgaat tagaatgtgg ccttactcct gggcaactca
    27721 tactcataga ttggactcaa aaaacaggga gaaattataa tttcccaaga tattttaaat
    27781 actctcttca aattgaccct gaatctacac acaatcaatt atacaaatta ggatacttca
    27841 ctaaaaataa gactttatca tatcttacag tagtagaatt aaaaactata ttatctaaac
    27901 ataatttagc tacttctgga aaaaaagcag aattaattac aagaataatt aataatgtta
    27961 acattgacaa tttagatatt ccgttcgaat ttaaactaac aaaagaagca caaaatctta
    28021 ttatcgaaca tagtgactat atcaaagcat actatgataa agacataagt atggaagatt
    28081 attgtaaaga aaaaaacaat atctctttta aagcaacttt tggtgatata aaatggagtc
    28141 tcttaaataa acaagctcat aggaatactg tatcaggaga ttttggatgc ttatctaaca
    28201 cacgaaaggc tcagggaaga catttggaac aagaaggtaa tattaaacat gctttaatat
    28261 attacataga atctttgata attactattt caggattaga aaacaatttt tcagccactg
    28321 attatccagt atattatccc gattcgatac ctgactactc actaaaacat attcaaacat
    28381 taatggaatc attatctgat gacgattatg attttgcttt tgatgaagca ttatttcgct
    28441 tctcaatttt gaatgcaaat cattttttat ccaaggaaga tattgactat ttaagagtta
    28501 atttacctcg ttccactgct gaagaaataa acaattactt aaagaaatat gaatgttata
    28561 gtcctttaaa taatttagaa cttgacgatt ttgaataaat tgactataca aacatttata
    28621 tactcgatat agtctcaatt ttatctgatg attgcccaaa tttttcaata ataaaacgca
    28681 taatattatg gagacaatcc cctatattat gcgttctttt aatatcaaag actctttgac
    28741 aaacttcttt gatatctaat tacatgcccc ctgcaggaat cgaacctgca actactcctt
    28801 aggagggagt tgttatatcc attgaactaa gggagctaga taaaaactct gctgaaaaag
    28861 cagagttttt tagtcgaatt aacgacggat ttctttgata cgagctgcct taccttgaag
    28921 agcacgcaag tagtacaatt tcgcacgacg tactttaccg taacgtacaa cttcgatttt
    28981 ttcaacacgt ggagtgtgga ttgggaagat acgctcaaca cctacaccgt tagagatttt
    29041 acgaactgtg tagttttcag agatgccagc acctttacgt gcaataacaa caccttcaaa
    29101 aatctggata cgttcacggt taccttcgac aactttcgca tgtacacgaa cagtgtcacc
    29161 aggacggaat gatgggatat ctgtacgaag ttgaccttca gtcaagcttt ggattaatgg
    29221 attcatttta ttctcctatc tttgtcaatc ttgaggaatc ttcctcagcg gataaactgt
    29281 atttttgtgc gtccattaca cacaagatat agtttaccaa atttctaaaa aaaagtaaag
    29341 aaatttatag caaaattcct aaaaaaatcg caaccaaacc acctatgtaa gtcaaagtca
    29401 ggtaagaata aaagaccttc ttatcactta gcagtctttg aagttcgtca ttcaaggtcg
    29461 aaaaagttgt taaaccgcca caaaatcctg ttgctagaat agcatatact tccttagact
    29521 ctacatgatt gtagaatact ccaattaaaa aacaacctaa aagattagct atgagcgttc
    29581 cgagtggcaa tttagaagct tgattatagc gagaaaagaa ataccgtacg agtgctccca
    29641 agccacaggc gattgcaaga tagacgatta ccatttcttc ctccccaaat agtaagctaa
    29701 aagcaggcct ccaccgatac tcaaaagcaa atacaggact aaactaaaat aacgccctgt
    29761 atcaagcagc ttcacagtat caagcattag gctggaaaaa gttgttaaac ctccgcaaaa
    29821 acccgtcccc agtgctaaaa tcaagccctt actagtcccc ttatagacca gatagccctt
    29881 gacaagaaaa atcaagcaga aaattcccag atagttgaca aagagagtgc cccaaggaaa
    29941 gtctggactg gctggtaacc aggtggaaac tagatatcgg acaagtccgc ccaacatagc
    30001 agctagaaaa attcctagcg gataaaattg ttcttttttc atttgatatt ttgatcctga
    30061 taatcacgcg aacgtttgag tatgtacgaa aaagtcgcga caatagtctc ctgatagcgc
    30121 ttgtcctcta cacgactcct aaccttttca aaaataactt cttctctctt agtatctaaa
    30181 atcggtttac ctgaagcttt cttataagca accaccccct caactaaatg cattcgttct
    30241 tctaacagtt tgacaatttg gtcgtcgatt tgatcgattt cttgacgaat aatatctaaa
    30301 tccatacagt ctcctccttt atttgaatta ttgtatcaaa aagccatcaa ttaggctagt
    30361 aaaatcccaa ctctcgataa gaaaaatgca ctgattgatt acatcagcgc acttttatgc
    30421 ttatcctact ttagcagcca attcttctgc gaattgttcc aagcgttcaa tatcttcttc
    30481 ctcggcagaa agatcaactt taacacactc tgaacctttt tctgctcctg ttgacacaaa
    30541 gacacggtca aagtcatcaa cagccttaca gaattcatcg tagaaggtat ctcctgagcc
    30601 gaccactccg tagattttgc cattcaagtt gagatctgct aggtcttcgt agaagtccat
    30661 catctcatct ggcaattcac catcaccata agtataggtc gcaacgatag cgatgtctgc
    30721 ttccaagaag tctgaagcgt caacagttgt acattcatca acatcaacat ccaagcccaa
    30781 gtcacgtaat ttgtctgcta caatatctgc aatttcttcg gtattaccgg tcatactggc
    30841 aaatacaatt tttgctaatg ccatatcgtc ctcctcaatt tatctttctc cattatatca
    30901 catctttttc attttaaaaa taaaaattct tgtgctacaa tgaaatgaga aagaattgag
    30961 gttatttatg gagatttgcc aacaaatttt agaaaaaatt aaagaataca acaccattat
    31021 cattcatcgt catatgaaac cagaccctga tgccttggga agtcaggtgg gattgaaagc
    31081 cttgctggaa catcatttcc cagaaaaaac catcaaagcc gtcggttttg atgaaccaac
    31141 tcttacttgg atggctgaga tggatcttgt tgaagataga gcctaccaag gcgcacttgt
    31201 catcgtctgt gatacagcta atactgctcg tatcgatgat aagcgctata gtcaaggtga
    31261 ttttctcatt aagattgacc accatccaaa tgatgatgta tacggtgacc tgtcttgggt
    31321 cgatactagt tcaagtagcg ctagcgagat gattacccta tttgcccaaa caacccaact
    31381 agccttggca gatcgcgatg ctgagttgct ctttgcagga attgtcggtg atacaggtcg
    31441 cttcctctac ccttctacca ctgcacggac tcttcgcctg gctgcttatt tgagagaaca
    31501 taactttgac tttgcggctc tcactcgcaa aatggacact atgagctaca aaattgctaa
    31561 actgcaaggc tacatctacg accatctgga agtggatgaa aatggtgctg ctcgcgttat
    31621 cctgagtcag aaaatcttga aacaatacaa tgtaaccgat gctgaaactg cggccattgt
    31681 aggtgcacct gggcgtattg atagtgttag tctctgggga atttttgtgg aacaggctga
    31741 tggccactac cgcgttcgct tacgcagtaa aatccatcct atcaatgaaa tcgccaagga
    31801 gcatgatggt ggaggccacc ctctagcaag tggtgctaat tcctatagcc tagaagaaaa
    31861 tgaaatcatc taccaaaagt taaaaaactt gcttaaaaac tgataaaata cttgccaaac
    31921 ttttcagaat ctgatagact agtatggtaa caatctatgg ctcgcaaaga gaccatggca
    31981 gaaaggaaat attgcaaaat gaaaaaagat atccatccag aatatcgccc agttgtcttc
    32041 atggacacaa ctactggtta ccaattcctt agcggttcaa caaaacgctc taacgaaaca
    32101 gttgagttcg aaggcgaaac ttacccattg atccgtgtgg aaatttcatc agactcacac
    32161 ccattctaca ctggacgtca aaagttcact caagcagatg gacgcgtgga tcgtttcaac
    32221 aaaaaatacg gtctcaaata atgataaaag aacagtttcg actgttcttt tttgtttctt
    32281 gaaatcaact gctgttttca tgttccagac tcatctgtag gttcggtttc catgctacta
    32341 ggcaggaagg aaatagctgt ttcaacacgt ccataatgag ctgtactatt gtcacgaacc
    32401 acactttcat tgatggtcca agtggaattc attttcttaa aagcttctcg gactttttcc
    32461 aaatctttgg aggcaatggc ctgttccaag gcttcaaaac gaggacttat actcatctgc
    32521 tttcaaaaag cattctagtc catctccgat t
//
LOCUS       DAVBCR010000019        32440 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCR010000019 DAVBCR010000000
VERSION     DAVBCR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.019"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            121..858
                     /locus_tag="VQ508_000787"
     CDS             121..858
                     /locus_tag="VQ508_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2489818.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            855..2546
                     /locus_tag="VQ508_000788"
     CDS             855..2546
                     /locus_tag="VQ508_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2489819.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            2662..3225
                     /locus_tag="VQ508_000789"
     CDS             2662..3225
                     /locus_tag="VQ508_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2489820.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            3247..8934
                     /locus_tag="VQ508_000790"
     CDS             3247..8934
                     /locus_tag="VQ508_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2489821.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            9003..10724
                     /gene="pabB"
                     /locus_tag="VQ508_000791"
     CDS             9003..10724
                     /gene="pabB"
                     /locus_tag="VQ508_000791"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2489822.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            10725..11342
                     /locus_tag="VQ508_000792"
     CDS             10725..11342
                     /locus_tag="VQ508_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2489823.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            11392..12390
                     /locus_tag="VQ508_000793"
     CDS             11392..12390
                     /locus_tag="VQ508_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2489824.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            12517..13476
                     /locus_tag="VQ508_000794"
     CDS             12517..13476
                     /locus_tag="VQ508_000794"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2489825.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            13573..14412
                     /locus_tag="VQ508_000795"
     CDS             13573..14412
                     /locus_tag="VQ508_000795"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2489826.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(14460..14630)
                     /locus_tag="VQ508_000796"
     CDS             complement(14460..14630)
                     /locus_tag="VQ508_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2489827.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            14751..15635
                     /gene="miaA"
                     /locus_tag="VQ508_000797"
     CDS             14751..15635
                     /gene="miaA"
                     /locus_tag="VQ508_000797"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2489828.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            15628..16866
                     /gene="hflX"
                     /locus_tag="VQ508_000798"
     CDS             15628..16866
                     /gene="hflX"
                     /locus_tag="VQ508_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2489829.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            16859..17482
                     /locus_tag="VQ508_000799"
     CDS             16859..17482
                     /locus_tag="VQ508_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2489830.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            17497..18426
                     /locus_tag="VQ508_000800"
     CDS             17497..18426
                     /locus_tag="VQ508_000800"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2489831.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            18447..19202
                     /locus_tag="VQ508_000801"
     CDS             18447..19202
                     /locus_tag="VQ508_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2489832.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(19256..20164)
                     /locus_tag="VQ508_000802"
     CDS             complement(19256..20164)
                     /locus_tag="VQ508_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2489833.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            20228..20497
                     /locus_tag="VQ508_000803"
     CDS             20228..20497
                     /locus_tag="VQ508_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2489834.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            20497..20877
                     /locus_tag="VQ508_000804"
     CDS             20497..20877
                     /locus_tag="VQ508_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2489835.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            20966..21193
                     /locus_tag="VQ508_000805"
     CDS             20966..21193
                     /locus_tag="VQ508_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489836.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(21233..21958)
                     /locus_tag="VQ508_000806"
     CDS             complement(21233..21958)
                     /locus_tag="VQ508_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2489837.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            22120..23961
                     /gene="typA"
                     /locus_tag="VQ508_000807"
     CDS             22120..23961
                     /gene="typA"
                     /locus_tag="VQ508_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2489838.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            23981..24235
                     /locus_tag="VQ508_000808"
     CDS             23981..24235
                     /locus_tag="VQ508_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2489839.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            24601..24900
                     /locus_tag="VQ508_000809"
     CDS             24601..24900
                     /locus_tag="VQ508_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2489840.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            24971..26992
                     /locus_tag="VQ508_000810"
     CDS             24971..26992
                     /locus_tag="VQ508_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2489841.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            26994..27635
                     /locus_tag="VQ508_000811"
     CDS             26994..27635
                     /locus_tag="VQ508_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489842.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            27739..29091
                     /locus_tag="VQ508_000812"
     CDS             27739..29091
                     /locus_tag="VQ508_000812"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2489843.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            29093..30151
                     /locus_tag="VQ508_000813"
     CDS             29093..30151
                     /locus_tag="VQ508_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2489844.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            30161..31360
                     /gene="divIB"
                     /locus_tag="VQ508_000814"
     CDS             30161..31360
                     /gene="divIB"
                     /locus_tag="VQ508_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2489845.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT        10060 a         5835 c         7204 g         9341 t
ORIGIN      
        1 aaacctgtgg ctatcttcct agtttacttt ttgattttta ttgagtataa gaggtctttc
       61 ctaagcagtt agaggagaat tttgctatac tgatactagt aagtggtaaa ggagcagagc
      121 atgacctaca caatcttaat cgtagaagat gaatatctgg taagacaagg tttgactaaa
      181 ctggtcaatg tagcagccta cgatatggaa atcatcggtc aggctgaaaa tggaaggcag
      241 gcttgggaat tgatccaaaa gcaggtgcca gatatcattt taaccgatat caacatgcct
      301 catctaaatg gcatccagtt ggccagtctg gtacgagaaa cctatcctca ggttcatttg
      361 gtctttttaa caggttacga tgattttgat tatgccttgt ctgctgtcaa actaggtgtg
      421 gacgactacc tgctcaaacc cttttctcgt caggatattg aggaaatgtt ggggaaaatc
      481 aaacaaaaac tagacaagga agagaaagaa gagcagttac aagatttatt gactaatagg
      541 tttgaaggaa acatggccca gaaaatccag tctcatctgg ctgatagcca atttagttta
      601 aagtctttag ccagtgactt aggttttagt ccgacctatc tgagttcctt gattaagaaa
      661 gagttgggct tgccttttca ggattatctg gtgagagaac gtgttaaaca agccaagctc
      721 ttgcttttaa ctacagatct gaagatttat gagatcgcag agaaggttgg ttttgaagat
      781 atgaactatt ttacccaacg ttttaagcag attgcaggtg tgacacctcg tcagtttaag
      841 aagggagaag accgatgaag cgttcttctc ttttagttag aatggttatt tccatctttc
      901 tggtctttct cattctccta gctctggttg gaactttcta ctatcaatca agttcttcag
      961 ccattgaggc caccattgag ggcaacagcc aaacgaccat cagccagact agccacttta
     1021 ttcagtctta tatcaaaaaa ctagaaacca cctcgactgg tttgacccag cagacggatg
     1081 ttctggccta tgctgagaat cccagtcaag acaaggtcga gggaatccga gatttgtttt
     1141 tgaccatctt gaagtcagat aaggacttga aaactgttgt gctggtgacc aaatctggtc
     1201 aggtcatttc tacagatgac agtgtgcaga tgaaaacttc ctctgatatg atggctgagg
     1261 attggtacca aaaggctatt catcagggag ctatgcctgt tttgactcca gctcgtaaat
     1321 cagatagtca gtgggtcatt tctgtcactc aagaacttgt tgatgcaaag ggagccaatc
     1381 ttggtgtgct tcgtttggat atttcttatg aaactctgga agcctatctc aatcaactcc
     1441 agttggggca gcagggcttt gccttcatta tcaatgaaaa ccatgaattt gtctaccatc
     1501 ctcaacacac agtttatagt tcgtctagca aaatggaggc tatgaaaccc tacatcgaga
     1561 caggtcaggg ttatactcct ggtcacaaat cctacgtcag tcaagagaag attgcaggaa
     1621 ctgattggac ggtacttggc gtgtcatcat tggaaaagtt agaccaggtt cggagtcagc
     1681 tcttgtggac cttgcttggg gccagtgtca catctcttct tgtttgtctc tgcttagtgt
     1741 ggttcagtct taaacgctgg attgctcctt tgaaggattt gagagaaacc atgttggaaa
     1801 ttgcttctgg tgctcaaaat cttcgtgcca aggaagttgg tgcctatgaa ctgagagaag
     1861 taactcgcca atttaatgct atgttggatc agattgatca gttgatggta gctattcgta
     1921 gccaggaaga aacgacccgt cagtaccaac ttcaagccct ttcgagccag attaatccac
     1981 atttcctcta taacactttg gacaccatca tctggatggc tgaatttcat gatagtcagc
     2041 gagtggtgca ggtgaccaag tccttggcaa cctatttccg cttggcgctc aatcaaggca
     2101 aggacttgat ttgtctctct gacgaaatca atcatgtccg ccagtatctc tttatccaga
     2161 aacaacgcta tggagataag ctggaatacg aaattaatga aaatgttgcc tttgataatt
     2221 tagtcttacc caagctggtc ctacaacccc ttgtagaaaa tgctctttac catggcatta
     2281 aggaaaagga aggtcagggc catattaaac tttctgtcca gaaacaggat tcgggattgg
     2341 tcatccgtat tgaggatgat ggcgttggct tccaagatgc tggtgatagt agtcaaagtc
     2401 aactcaaacg tgggggagtt ggtcttcaaa atgtcgatca acggctcaaa cttcattttg
     2461 gagccaatta ccagatgaag attgattcta gaccccaaaa aggaacgaaa gttgaaatat
     2521 atataaatag aatagaaact agctaactcc cagtcaattc tgggagtttt atgcgtaatt
     2581 gggcagactt tataggttgt ctatcttgct aaaaacaaga aaaaacggta tgatagaaga
     2641 gtgacaacag aggtaacaga gatgaacgag acacagattc aaagagaaac acgtcaggta
     2701 gtggaagatg ttttagaaaa gaccaatttg aagcaggggg tcctttttgt tttaggtcta
     2761 tcttctagtg aggtgctagg tggtcagatt gggaaagaat ccagtcaaga gattggtgag
     2821 ctgatagtgg agactatctt gggtattcta ggtagtagag gtatccattt agcagttcaa
     2881 ggttgtgagc atgtcaatcg ggctctcgtt gttgaacgtc aggtggcaga gcagtttgat
     2941 ctggaaattg tcagtgtcca tcctactctt catgcaggag gttcggggca gttggcagcc
     3001 tttaagttta tgcaggatcc agttgaggtt gaatttatca aggctcatgc tggattggat
     3061 atcggagaca ctgcaattgg catgcatgtc aagcatgttc aggttccgat tcgccctatt
     3121 ttgagagaga ttggtcatgc ccatgtaacg gcactggcta gtcgtccaaa attaatcgga
     3181 ggtgcgcgtg cgcactatcc gcaagatgct attagaaagt cttgagaatg aataaaggag
     3241 atgaaagtgt ttaaaaaaga ccgtttttca attcgtaaga ttaagggagt tgtaggctct
     3301 gtatttcttg gaagcctttt gatggctcct tctgtagtgg acgcagctac ctatcactat
     3361 gtaaataaag agattatttc acaagaagct aaagatttaa ttcagacagg aaagcctgac
     3421 aggaatgaag ttgtatatgg tttggtgtat caaaaagatc agttgcctca aacagggaca
     3481 gaagcatctg ttttgacagc ttttggtttg ctgactgttg ggagcttgct tttaatctac
     3541 aagagaaaga aaattgctag cgtctttcta gttggagcta tgggattggt agttcttcct
     3601 agtgcagggg ctgtagaccc agttgcgacc ttagcgctgg ctagtcgaga gggtgttgtt
     3661 gaaatggatg gctatcgcta tgttggttat ctatcaggtg acatcctcaa aacgcttggc
     3721 ttggacactg ttttagaaga aacctcagca aaacctggag aggtgactgt agtcgaagtt
     3781 gagactcctc aatcaacaac aaatcaggag caagctagga cagaaaacca agtagtagag
     3841 acagaggaaa ctccaaaaga agaagcacct agaacagaag aaagtccaaa ggaagaacca
     3901 aaatcggagg taaaacctac tgacgacacc cttcctaaag tagaagaggg gaaagaagat
     3961 tcagcagaac cagctccagt tgaagaagta ggtggagaag ttgagtcaaa atcagaggaa
     4021 aaagtagcag ttaagccaga aagtcaacca tcagacaaac cagctgagga atcaaaagtt
     4081 gaaccaccag tagaacaagc aaaagtccca gaacaacccg tgcaacctac acaagctgag
     4141 caaccaagta caccaaaaga atcatcacaa gaagacaatc ctaaagaaga taggggagcg
     4201 gaagagacac cgaaacaaga agagtcaact ccagatacca aggctgaaga aactgtagaa
     4261 ccaaaagagg agactaaaac agctaagggg acgcaagaag aagattacgt gggtgaggct
     4321 cttgttccag aacaattacc tgagtataag gtgactgaag gaacactcgt tgaagaatca
     4381 actacagaac ttgactataa aactgaaacg actgaggacc caaccaagta cacggacgaa
     4441 gaaactgtcc tacgaaatgg tgaaaaagga agtcaagtta caaaaacaac ctataaaaca
     4501 ataggagggg ttaaaactga ccaagttctt tcaaccagta cggaagtcac caaggagcct
     4561 gtaaatcaac aggtaatccg tggtacaaaa ccaattgaag ggacaacggt tgagacaaga
     4621 gaagaaatca ttccttttga aacaaaagaa caagaagatg acactttaaa acgcggaaca
     4681 agacaagtaa ctcaagaagg tgtaaacgat aagaaacaaa ttactgaaac ctataaaaca
     4741 attcgcggag aaaaaacaag tgacgctcct actatcacag aaacagtgat tgaaaaacct
     4801 caagacaaaa ttattaagaa tggcacaaaa gagttagaga aaccaacctt ggaatgggct
     4861 aaaacagata aagatgtctt gaagaagagt gcgacagcta gttatacatt gactaaacca
     4921 gctggagtag aaattaaatc gattaaagta gctctaaaag ataatactgg aacagttgtt
     4981 aaagaagtaa cggtagaaga aaataactta caagctactt tagacaactt gaaatattat
     5041 caaggttata cactatccac aaccatggtc tatgatcgag gtaacggtga agaaactgaa
     5101 atattagaag ataaagaagt tcagttagac cttaaaaaag ttgagattaa agatattaaa
     5161 gaaacaagat tgatgaaagt ggatgagaat ggaaatgaaa cagatagtag ccttctagaa
     5221 actgttccag aaaatctaac gtcatattat ctaaaaatta ccactaatca taataaagta
     5281 acaaggcttg ccattactaa tatagaggaa gtaactgagg aggagacatc cttatttaaa
     5341 gttactgctt cagctcctga tttaatccaa cgtgattcaa ataatagttt gagtcaaaca
     5401 tacacttatt acatagagaa acccaaagca tcgcaaaata acgtttacta taacttcaaa
     5461 gatttagtag atgctatgca gaaaaatcct aacggtgagt ttaaattagg atcggattta
     5521 aatgcaacaa atgtacctac accaagtaaa tcatatgtaa caggtaaatt tacaggtcac
     5581 ttaacaagtg ttgatggtaa gcatttctcc attcataaca ctgctcgtcc attatttgat
     5641 agagttgaag ggggttctat caaaaatatt aatttaatta acgttaatat taatatgcct
     5701 tgggcaaatc aattagcttc attagctcaa acagtgaaaa atgcaactgt tgaaaatatt
     5761 aaagtaacgg gtactatcgt tggtaataac aatgttgcgg gtattttgga taaattagat
     5821 gatgcaggta ttatgactaa tgttgctttt atcggaaaag ttcaatctgt tggtgataaa
     5881 gggtggaact tggctgggat tgcatctgag atttatcgag gcaatttaaa taaagcttat
     5941 gcagatgttg aacttaaagg tttaaatact cgtgcaggtg ctatagtagg acatttagct
     6001 catgatttta atccaagaga tgttggtgct tggtctaatg taagaaattc tgtagccaaa
     6061 ggtactattg atgttgctag aggtgatgta ggcggtttaa ttggtaaaga ctggccttgg
     6121 ggaaccattg ataacaatat cagcttagtg aatgttaagc gtggtgatat tttctttggt
     6181 tctaatgata tggatgatga atttgtaagc aattggatta aacctaataa tgcaacagtt
     6241 atcggtgtga gttctggaga taaaacccat aaacaatcgt ctaaaattaa agaaatttca
     6301 caggtagaag ctgataagaa aattaaggat atgggtatca ctgctgatac atttgaagta
     6361 actgagcatt tggttgataa gttgaatcgc cgtatattac gtgatgaaga atacaaaaca
     6421 attcaagatt ataatgcaaa tcgtgagctt gtttatcgta atgttgaaaa actccaaccg
     6481 ttctacaaca aagaatggat tgtgaatcaa gggaacaagc ttacaactga tagtccatta
     6541 atgaataaag aagtattgtc tgtgactgca atgaaaggta atgactttat tacagacctt
     6601 acagatgctg accatattat ggttcattac gcagataaga caaaagatat ctttacgatt
     6661 tctcctaaag actctcaagt taagcaagta aaagaataca gtgtcgcaga gcttggtgaa
     6721 gtcgtctata caccaaatat ggttgttaaa gatcgtgcag atttaattag tgctattgaa
     6781 ggtattttaa gtccaattga attacaatct gatccaattt atcaacgatt aggaagaact
     6841 ggggataaca gagttaatgc aattaagaat ctttacttag aagaaagctt taaagaagta
     6901 aaagatcatc ttaatacatt tgtgacaaaa ttagttgaaa atgaagatca tcaactgaat
     6961 acagatgaag ctgcaaaacg tgccttgatt aagaaaattg aagataacaa agctgctgtc
     7021 ctgttaggat taacatacct aaatcgttat tacggtgtga aatttgataa cttcaatatt
     7081 aaagaattaa tgctattcaa accagatttt tatggtaaga atgtaagtgt tttagatcga
     7141 ttaatagaaa ttggttcaaa agaggactat attaagggaa gtcgtacaca ggagtccttc
     7201 cgtgaagtgg tggcgaaaaa tactctttct ggaaacttac atgacttctt gacctacaat
     7261 atgaaactat tcacaaatga aacagacata aatgtttggt ttaagaaagc tattgaaaat
     7321 aatgcttatg tagttgaaca accgtcaact aaccctgcat ttgctaataa aaaatatcgt
     7381 ctatatgaag gacttaataa cggacaacat ggacgcatga ttttatcgct tctgaacttg
     7441 aaagatgcac atttatttat gatttcaaca tataatacta tctcatttag ttcgtttgaa
     7501 aagtatggta aagatacaga agaaaaacgt gaatcgttca agagtgaaat caataaacgt
     7561 gctaaagagc aagttaatta cttagatttt tggtcacgcc ttgccactga taatgtccgc
     7621 gataaacttc ttaagagtca aaatgtagtt ccaactcctg tatgggataa tcatggtgca
     7681 cccggtggtt ggcctgatag atttggtcat cgaaaagata aaccggatta tgcacctatt
     7741 cgtgaattct ttggacgtat tgataagtat catgattata agtatgggta tggagcgtat
     7801 gcttacatct ttgctgatcc acagcctatg gatgctgtat attttgttat gtcggattta
     7861 ataagtgaat atggcacatc tgcctttaca catgagacta cccacgtaaa tgaccgaatg
     7921 gcatatctgg gaggtcatag acatcgacag ggtacagatc ttgaagcatt tgctcaaggt
     7981 atgttacaaa ctccagcaga acatggacat caaggtgaat atggcgcact tggattaaat
     8041 atggcatttg aacgttcaaa tgatggaaac caatggtata atccagatcc taccaaactt
     8101 caaacacgtg accaaatcga ccattacatg aagaactaca atgaagctat gatgatgctc
     8161 gattatgcag aagctgaggc tgttcttcct aaggttaaag gtgacaatag taagtggttc
     8221 aagaaaattg atcgcgaaac acgtcgacca atggacagaa ataaactaag tggtcctcac
     8281 caatgggaca aagtgcgtga tttgatagac gctgagaaaa caactaacct tgaaaccatc
     8341 gatgacttgg taaataataa tttcatgacc attcacggaa atccaggtaa taaggtcttc
     8401 cacccagagg actttggtac ggcatatgtc aatgttaata tgatggctgg tatttatggc
     8461 ggtaatacga gtcaaggtgc ccctggttct ctatccttca aacataacgc cttccgtatg
     8521 tggggttact atggatatga acatgggttc attgactatg tttcaagcaa acatcaaggt
     8581 gcagccaata aagaaaataa cggtctatta ggtgatgatt tcattattaa gaaagtttct
     8641 aataaccaat tttcaaacct tgaagattgg aagaagcaat ggtatgggga agttcttaca
     8701 aaatctaaaa aaggttttgc accaattgaa attaatggca ctcaaatttc aacctatgaa
     8761 cagttgaaga ctatgtttga tgaggctgtt actaaagatt taacggaact aaacgatagt
     8821 agtattaaag acaaataccg aaatacagtt gtcttgaaat cgaaagtctt taaagctctc
     8881 cttaaaaaca cagacggttt ctctggtgat ctatttactg caccacaagc atagaatgaa
     8941 taaaatcgta aaaaggtaga tcagctttgt ctgtatctgc ctttttatgc tatacttaag
     9001 gtatgcatag aaaaacagtg attgatttta ggactttagg ggagagatat acctttgccc
     9061 agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg ctggcacaag
     9121 tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac
     9181 ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt
     9241 actttactgt tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg
     9301 atttgccctc aaattggcag gaagtgacgt ctgcagcaga ctatgaaaag gccattgccc
     9361 agatacacca tcacttgagg cagggagaca cctatcaggt caactacacc gtccaactca
     9421 agcaaaagtt aagtgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg
     9481 cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc
     9541 tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc
     9601 gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa
     9661 atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg
     9721 aagttgggag cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca actgtttggc
     9781 agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc
     9841 gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta
     9901 tcaaggactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc
     9961 caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag
    10021 ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct gaataccgag
    10081 aggttcatca aaaggcagca gttctttatc gtaaacaagc tcgcttccaa ctgattacga
    10141 caggggaaat cagccagaag aacctgctgt ttgaagatca acatctggaa agactgagaa
    10201 aagctagtcg ttattttgct tttccttttg atgcagaaga cttgggacac aagattgagg
    10261 aagagtgtca ggattgtgaa gctaatcgag attaccgctt gcgaatcagc cttagcaaat
    10321 ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc ttttgtcagg
    10381 cccaagtctg ccttcaggaa gctgctttga atcaatcctt tacctacttt aaaaccactc
    10441 accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag tcaggagaac
    10501 tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc tacacaccgc
    10561 ctatccgact tggaatctta ccaggaattt accgtcagta tttgctagaa acaggacagg
    10621 tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt tacggctgta
    10681 atgcagtgag aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa ttgatatttt
    10741 tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta ctgacagatt
    10801 atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa caagctaaag
    10861 agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt ggattgtcct
    10921 taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt cgagctttga
    10981 ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt cagttgctga
    11041 tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag actcttatgg
    11101 tcggagtttc tgaggaattg aagacacttg atttaacgga tatagcaggg acttgctcct
    11161 atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct atgaaagctc
    11221 tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct catgtcttga
    11281 ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa ttgctgaaat
    11341 aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga aatgaataaa
    11401 cgtctatttt taaaaatgag tctggtgacg ttgccaattt tagccttgtt ttcacaacca
    11461 gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc taatggatat
    11521 tcggatcttc atgagggaga acctggttat tctgccaagt tagaccgcga tcatgatggt
    11581 gtggcttgcg aattgaaaaa tgctcctaag ggtactttta aagcaaaaca agcggctaca
    11641 actcaaactg atacaacttc atccacagca agtggttggg ttaagcagga cggttcttgg
    11701 tactattttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaaa ctattacctg
    11761 aaagctgatg gcaaaatggc acagagtgaa tggatttatg actcttctta tcaagcttgg
    11821 tattatttga aatcagatgg ttcttatgca aaaaatgcat ggcaaggagc ttattacctt
    11881 aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta ccaagcatgg
    11941 tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa ctactatttg
    12001 aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta tcaagcttgg
    12061 tattatttga catcagatgg ttcttatgct tatagtacat ggcaaggaaa ttactatcta
    12121 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
    12181 gctgacggag tttggaagga aggtcaagca agtacagctt cttctagtaa tgatagcaat
    12241 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
    12301 aaaaaacgta tgtatagaca attaacttct gattttgata aattttcaaa tgatgcagct
    12361 caatatgcca ttgatcattt agatggttaa cagatactgg taggcgaaaa aattccttat
    12421 aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc
    12481 attttcaatt actacacaat gaaaaaggag aatataatga gtcaaaagat tattgggatt
    12541 gaccttggtg gaacttctat caaatttgca atcttaacaa cagcaggaga aatccaagga
    12601 aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt
    12661 gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt
    12721 ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg tgcctacaac
    12781 ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt gggcattcca
    12841 tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctggt
    12901 gataaccaac cagatgttgt ctttatgaca ctcggtactg gtgttggtgg cggtatcgtc
    12961 gcagaaggca aattgcttca cggtgttgct ggtgcagcag gtgagttggg tcacatcact
    13021 gttgactttg accagccaat ctcatgtact tgcggtaaga aaggctgtct tgagacagtc
    13081 gcttctgcaa caggaattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat
    13141 gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac tgtctttgat
    13201 ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc acgttacttg
    13261 ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt
    13321 ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagtcta tgacgaaaat
    13381 actttcccac aagtacgcac atctactaaa ttagctcttg caactctagg aaatgacgct
    13441 ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa
    13501 cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg
    13561 aggtgagagt aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta
    13621 aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat
    13681 tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc
    13741 ataaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa
    13801 gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta ctggaatgac
    13861 tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt taagaaacac
    13921 gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg ccgcaatatt
    13981 atttctctct gggattacca agctttcgaa gaaacagatg ggctgctccc gtgcgccttt
    14041 cagaccatgt ttgatgttcg gcgtgttgat ggggaaatct atctggatgc gaccttgacc
    14101 cagcgctcca atgatatgct ggtggcccac cacatcaacg ctatgcagta tgtggctttg
    14161 cagatgatga ttgctaaaca ttttggctgg aaggttggga agttcttcta cttcatcaac
    14221 aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg tcgggagccg
    14281 tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt ctttgatatc
    14341 aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt gaagtttgac
    14401 ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct
    14461 taacgtgata cgcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt
    14521 tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg
    14581 acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct
    14641 cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt
    14701 tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa
    14761 aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg
    14821 caaagtgctt taatggcgaa gtggttagtg gagatagcca gcaagtctat cgaggacttg
    14881 atattgggac ggccaaggct agtccagaag agcaggcagc tgttcctcat catttaatcg
    14941 atgttagaga gataactgag tcttactcgg cttttgattt tgtttcagaa gctaagatga
    15001 ctattgagga tattcacagc cgtggcaagc tagccattat cgccggtggg actggacttt
    15061 atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt
    15121 tagcttatcg agctagtttg gagccatatt cagatgagga attagcccat ttggtggagc
    15181 aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg
    15241 cccattttgg tcaggatttg gaaaatcaag agattttgta tgaaccgctg attatctgct
    15301 tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg
    15361 aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg
    15421 ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag accttggagg
    15481 aagctcgtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt
    15541 tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta caagaccgca
    15601 ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc
    15661 ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg
    15721 gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa
    15781 aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg
    15841 gatgcagaag aaatcactac tgtcatcgtc aacaaccgtc tgaccccaag gcagaatgtc
    15901 aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat tttggatatc
    15961 tttgccatgc gggctcgaag ccatgaaggg aagctccaag tccacctagc ccaactcaaa
    16021 tacctcttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt
    16081 ggttcccgtg gtcctggtga aagccaactg gagctgaacc gtcgtagcgt tcgcaatcaa
    16141 atcacggata tcgagcgcca gcttaaggtg gttgagaaaa atcgtgcgac tgtcagagaa
    16201 aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttatactaa tgctgggaaa
    16261 tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg
    16321 actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt aactttgaca
    16381 gatactgttg gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc
    16441 ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac
    16501 cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt
    16561 cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg
    16621 ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg
    16681 ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc
    16741 tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac
    16801 ggcgaagtga ttacaggcta catttccgag aaaaataaat ggaggttaga agaattttat
    16861 gactgatatt aaaactttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta
    16921 tctggatcag cttctagctg gtaaaacaga gcaggagcag ttaacactta ttacacctcc
    16981 gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaaaagtc ctcaggctgc
    17041 gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt
    17101 caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg
    17161 ttatgagagt gacggctttg gtcggatttt ctctgaaaat gaggagaaaa tctcggatga
    17221 cttgctcttt gaaattgcgc aaattttctc ccatcaatta gtctttgaag agtctggtaa
    17281 gatttacatg aagcctgtca aggacgagga agttgtcagc ctagaaagtc tcacagcttt
    17341 gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga
    17401 tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac
    17461 agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg
    17521 ggggctggtc agccctctaa agcccgcaac gtttcaagtc tcgccctgaa acttttggac
    17581 gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa
    17641 accacaattc gaccacgtaa ggtcagcaaa atctttatta ctcatctgca tggagaccac
    17701 atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca
    17761 gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg
    17821 tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctgggt
    17881 aaaattcttg aaaccgataa attcactgtg tatgcagagg agctggacca cactattttc
    17941 tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc tgaaaaactc
    18001 aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt
    18061 gttttggaag acggaactga aatcaaggca gcagactata tctcagcgcc acgtccaggt
    18121 aagattatca ctattttagg agacactcga aaaacggatg ccagtgtgcg tctggctgtc
    18181 aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt
    18241 aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa
    18301 cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatataag caaactcaag
    18361 aaggacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa
    18421 atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg
    18481 gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa
    18541 ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg
    18601 atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca
    18661 agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt gaccagattg
    18721 ctgataaaga tattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc
    18781 gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca
    18841 tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta
    18901 ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag
    18961 tcaatccagg tccaatccga acaggatttt ttgaccaagc tgacccagat ggaacttatc
    19021 ttaaatcggt tgaccgcttc ctcttagagg cagatgcagt ggctaaaaag attgtcaaga
    19081 ttataggaaa aaataaacga gaactcaatc tcccgatttt gttgaaccta gcccataagt
    19141 tttatactct ctttcccaag ctagctgata agttggcagg ggaaactttt aattataagt
    19201 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
    19261 tatcaaactg aacttcttct agtagatagt cgataaagcg ttcgcccatc ttagataggc
    19321 tggttttctc atgctggata tagaccagct caatcgggtc atcaatatcc agtgggatag
    19381 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
    19441 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
    19501 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
    19561 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
    19621 ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg gctagtaggt
    19681 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
    19741 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
    19801 ggaaaagctc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
    19861 caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca ggatttttat
    19921 agcgttcctc cagaagctga gtttgctcga caacctgacg ggcataagag agaaactcca
    19981 tcccatcacg ggtcaaggtg attcccttgg gattgcggat aaagatctca atgcccattt
    20041 cattttccaa atctctcaca gcattggaaa gacttggctg agtgataaag agttgcttgg
    20101 ctgcctcatt catagagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
    20161 tcatgttttt attgtaccac acttgcctta gaatcgccaa agaagatagg aaaataagga
    20221 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
    20281 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
    20341 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
    20401 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
    20461 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
    20521 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg tcgtgcggct
    20581 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
    20641 cgcgacccag cagaattcca cagaaaacat atccttggtg cacgcaatat tccttcaagt
    20701 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
    20761 caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag
    20821 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
    20881 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
    20941 ataaaattta aagagttctt agaacatgaa agaaaagaag aaaaatccac tttttgtggg
    21001 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcttgattt tgggtatcat
    21061 aggattggtc ttggctattt cataccaaaa agagtcacaa ttagactata aaatagaaaa
    21121 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
    21181 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac
    21241 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
    21301 tttctgctaa atctgggttc aatgtgaagt ccccaaattg gattcttttg aggctagtca
    21361 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
    21421 tgatagaggc ttgactgagg gagggacttg cagatagaat ctctagtttt gcgggtttac
    21481 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
    21541 gaagttcatt aacctggact tggtaagtct tatcgacatg atattgggga tggaggagct
    21601 gaaagcccaa gggaccgtta tcggtcaaga ggaggagtcc cgttgtatct cggtccagtc
    21661 ggccaacggc atagagcttg tcagactgga tgtcagatgg aagcaggtcc atgacggtcg
    21721 gaagttcctt gtctttgttg gctgtaacga caccagcagg cttatgaagc ataagatagg
    21781 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
    21841 tattttgagc tagggatcgg gctgggcaac catcgactag aatttcccct ctgaggagtg
    21901 cttgcttcat ggcctttcgg ctaacttttt cttgggctaa taaattatct aaacgcatac
    21961 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
    22021 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
    22081 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
    22141 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
    22201 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
    22261 tcaaacgata tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac tgccgttgct
    22321 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt tggtggagaa
    22381 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
    22441 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
    22501 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
    22561 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
    22621 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
    22681 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
    22741 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
    22801 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
    22861 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
    22921 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
    22981 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
    23041 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
    23101 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
    23161 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
    23221 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
    23281 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
    23341 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
    23401 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
    23461 gttccagcgc gtggtttgat tggatactca actgagttct tgtcaatgac tcgtggttac
    23521 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
    23581 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
    23641 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
    23701 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
    23761 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
    23821 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
    23881 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
    23941 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
    24001 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggacagtcaa
    24061 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
    24121 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta
    24181 ctttagtgtt agagacatta cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
    24241 agagttttgg ctctcttttt tgctaggaat tttacaatag tttgaattgt tttgtactat
    24301 aacatcatct cttttgcttg gatagtatga atgggaaaaa cttgaataaa attcgttaat
    24361 ggcatctatt ttatatttgg ttagtactgg ttgcatatta ttttaaaata aaactactat
    24421 ataatattat tcatagaggc aatgtagtaa aatatctaaa aaattcgatt ttttccttga
    24481 caacaaataa taaaaatgat atttttatca cataagaaag cggtttcaac aaaagagaga
    24541 ggagggttta ttttgttgca ttgttgctca aggtgtgctt tttttgaaag aaaaaataaa
    24601 atgaaaacga aaaaacatag attacttgct ctagctctta tttcaagttt tacattattg
    24661 ggagctgcat cagctgctgt acaatatcca gatggaggag tatggacgta tggagaaggt
    24721 tcaggaggtg gttgggcttt ttcaaattac tatcatggta aaaaatatca ttattcttct
    24781 cttgtaagta gatggaatag tcattcagat aaaggagaag cttctgctgg aaaaacctcc
    24841 tatgcatgga tttggactaa atggggagaa caagtagcat tttactgtga ttatgactaa
    24901 tcgttcattg atttaaatcg ttccccttgt aagaaattac agggggaatt ttgtttacaa
    24961 ggagtctact atgaagaaac tattcatatt attatcaact ttttttctca gcttcttcct
    25021 tgcttggatt attgtcttac gtgcgccaca atatttatat gcaagctatg attccgtttc
    25081 tttacttcgt gtcaaaaaag atactcagga accgacgcgt gaggtatttg aacaggaatt
    25141 ggagaatttt gcaaactcag aacagagttt aatagctaga agaattgtag agccgagtaa
    25201 ggatgggacg actcacttta cttatgcaac ttatggtcag ggaactttac caaaagaatt
    25261 ccaagaagct agccaagaaa gtcgtgaacg tagtgatccg ctaaatagtt atctcctttt
    25321 gtcaggctcc ttgacgaaag aaaagcttgc cgataaatta ggagatttgg gttataaagc
    25381 aagtgctgac cgaaagatac cgccctattt tcttgctttt cgaatattac taaatcccct
    25441 tattttaatt agtttagcaa tatttggctt atctttcttt gctttagtga ttatcactcg
    25501 gattaaggaa atgagagcag caggtataaa actcttttct ggtcagactc tcttatccat
    25561 catggggcat tctttatcta ctgatatcaa atggctcctt ctatcagccc tcctttcctt
    25621 cctaggtggg ggagtcgttc tttttagtca aggtttgttt tatcctatct tgttagccac
    25681 ctatggtttt gggattagtt tctatctgtt gtttttattg gcgatttcaa ttttactaat
    25741 gcttctttat ctaatgagtt tgagttacaa agcattagtt cccgttatta aggggagatt
    25801 accccttaaa cgcctgatga ttttaaccct attgtgtcag ttggttgctg tctttacagt
    25861 aggctacgct gttaagacag gtttgatgtc ttaccaacga ttgaaagaac ttgaaatttc
    25921 aaaacaagca tggcaggata gagcagacta ttatcaaatt tcttttggct taggtgatag
    25981 aggaaaagat acagaaaatc agagcaagtg gtatgccttt gccaaggaag caatcgaaga
    26041 agaacaagct ctttatgtaa aggataatct gctccatttt gccaatccac aaggaaaaaa
    26101 tgaacaggga gagacactgg atacctatag tccagatgct aatacgctct atgttagtcc
    26161 cagttatttg gacaaggaaa aggtcgtggt agatgctgag accaaacaga agttagccca
    26221 tctccaaaaa ggtgagttta tcctcttgct cccagaacat ttgcgctctc gagaagcaga
    26281 acttaagaaa gtttttgaag aaagattgag ttattatgga aaatctggtg aggaggcaag
    26341 tgctcctttg gattatgaga tgaaagcgca cgttagttat ctttcaatgg gagaaaagcg
    26401 gtttgtttat aataacggtg agaatcccgt atctactcag tatttgactg atccgatttt
    26461 agtggtattc acgccgactt ctacaggtga tagttttata tccttatcta gttggtctat
    26521 caatgctgga aaacaactct ttatcaaagg atatgagagt gggctagaac tcttgaagaa
    26581 agctggaatt tatgagcaag tatcctatct taaagaagga agaagtgttt atctaactcg
    26641 ttataatgaa gttcaaactg aaacagcaac tttaatctta ggagctattg tggggatagc
    26701 tagttcctta ttactatttt attctgtcaa tcttctatat ttcgagcaat tccgccgaga
    26761 tattttgatt aaacgaattt caggtttacg attttttgaa acacatgctc agtatatggt
    26821 tagtcaattt gccagttttg tatttggtgc tagtctcttt attttaagca gtcgagactt
    26881 ggtgattggc ttgctcactt tattagtctt tctagctagt gcagttctga cgctttaccg
    26941 tcaagcgcag aaagaatctc gtgtttctat gacaattatg aaaggaaaat aggatgattg
    27001 aactaaagaa tatatctaaa aaatttggaa gccgtcagct attttcagat acaaatcttc
    27061 attttgaagg tgggaaaatt tatgccttaa tcggtacaag tggctgtggt aagacaacat
    27121 tcttgaatat gattggacga ttagagccat atgacaaagg acaaatcatc tatgatggta
    27181 cttctcttaa ggacatcaag ccttctgttt tctttagaga ttacttggga tacttatttc
    27241 aagattttgg cttaattgaa agccaaaccg tcaaagagaa tctcaatctg ggtttagttg
    27301 gtaaaaagtt gaaggaaaaa gagaaaatct ctttgatgaa acaagctcta aactgtgtaa
    27361 acctctctta tttggatttg aagcaaccta tatttgagtt atcaggagga gaagcacaac
    27421 gtgttgcatt agcgaagata attttaaagg atccaccttt gatcctcgca gatgaaccaa
    27481 ccacttcctt agaccccaaa aactctgagg aattactttc catcctagaa tctttaaaaa
    27541 atccgaatcg gaccattatt attgcgaccc acaatcctct tatttgggaa caagtggatc
    27601 aggtcattcg agttaccgat ttatcacata gatgatatgg taagattcag ttagaagaaa
    27661 gagtcacaaa cacactttgt ggctttttta tttccataaa aatggtaaaa tagtaggagt
    27721 agaaatggag ttcgagacat gaaagtaata gatcaattta aaaataagaa agtccttgtt
    27781 ttaggtttgg ccaagtctgg tgaatctgca gctcgtttgt tggacaagct aggtgccatt
    27841 gtgacagtaa atgatgggaa acctttcgag gacaatccag ctgcccaaag tttgctggaa
    27901 gaagggatca aggtcattac aggtggccat cctttggaac tcttggatga agagtttgcc
    27961 cttatggtga aaaatccagg tatcccctac aacaatccca tgattgaaaa ggctttggcc
    28021 aagggaattc cagtcttgac tgaggtggaa ttggcttatt tgatttcaga agcaccgatt
    28081 attggtatca caggatcgaa cggtaagaca accacaacga ctatgattgg ggaagttttg
    28141 actgctgctg ggcaacatgg tcttttatca gggaatatcg gctatccagc tagtcaggtt
    28201 gctcaaatag catcagataa ggacacgctt gttatggaac tttcttcttt ccaactcatg
    28261 ggtgttcaag aattccatcc agagattgcg gttattacca acctcatgcc aactcatatc
    28321 gactaccatg ggtcattttc ggaatatgta gcagccaagt ggaatatcca gaacaagatg
    28381 acagcagctg atttccttgt cttgaacttt aatcaagact tggcaaaaga cttgacttcc
    28441 aagacagaag ccactgttgt accattttca acacttgaaa aggttgatgg agcttatctg
    28501 gaagatggtc aactctactt ccgtggtgaa gtagtcatgg cagcgaatga aatcggtgtt
    28561 ccaggtagcc acaatgtgga aaatgccctt gcgactattg ctgtagccaa gcttcgtggt
    28621 gtggacaatc aaaccatcaa ggaaactctt tcagccttcg gtggtgtcaa acaccgtctc
    28681 cagtttgtgg atgacatcaa gggtgttaaa ttctataacg acagtaaatc aactaatatc
    28741 ttggctactc aaaaagcctt gtcaggattt gataacagca aggtcgtctt gattgcaggt
    28801 ggtttggacc gtggcaatga gtttgacgaa ttggtgccag acattactgg actcaagaag
    28861 atggtcatcc tgggtcaatc tgcagaacgt gtcaaacggg cagcagacaa ggctggtgtc
    28921 gcttatgtgg aggcgacaga tattgcagat gcgacccgca aggcctatga gcttgcgact
    28981 caaggagatg tggttcttct tagtcctgcc aatgccagct gggatatgta tgctaacttt
    29041 gaagtacgtg gcgacctctt tatcgacaca gtagcggagt taaaagaata atatgaaaaa
    29101 aattgtcttt acaggtgggg ggacggttgg acacgttacc ctcaatcttt tgttaatgcc
    29161 caagttcatc gaagatggtt gggaagtcca ctatatcggg gacaagcgtg gtatcgaaca
    29221 ccaagaaatc cttaagtcag gtttggatgt caccttccat tctattgcga ctggaaaatt
    29281 gcgtcgctat ttctcttggc aaaatatgct ggacgtcttc aaagtttgct ggggaatcgt
    29341 ccaatcgctc tttatcatgt tgcgacttcg tccacagacc cttttttcaa aggggggctt
    29401 tgtctcagta ccgcctgtta tcgctgcgcg tgtgtcagga gtgcctgtct ttattcacga
    29461 atctgacctg tctatgggct tggccaataa aatcgcctat aaatttgcga ctaagatgta
    29521 ttcaaccttt gagcaagctt cgagtttgtc taaggttgag catgtgggag cagtggccaa
    29581 ggtttcagat caaaaaaatc cagaaccaga tgaattggtg gatattcaaa gccactttaa
    29641 tcataaattg ccgactgtat tgtttgttgg cggttctgca ggtgctcgtg tctttaacca
    29701 attggtgaca gaccataaga aagaactaac agagcgctac aatattatca atctaactgg
    29761 agattctagc ctgaatgagt tgagccaaaa tctttttcgt gttgactatg tgaccgatct
    29821 ctatcaaccc ttgatggaat tggctgatat tgttgtgaca cgaggtggtg ccaatacgat
    29881 ttttgagctc ttggcgatag caaaattgca tgtcattgtg ccgcttggtc gtgaagctag
    29941 tcgtggtgac cagcttgaaa atgcagctta ctttgtaaaa aaaggctatg cagaagacct
    30001 tcaagaaagc gatttgacct tggatagttt ggaagagaag ctttctcact tactaagtca
    30061 caaggaagat taccaagcta agatgaaggc ttctaaggaa ttgaaatctc tagcagattt
    30121 ttatcaattg ttgaaaaaag atttatcata aggaaagtaa atgtcaaaag ataagaaaaa
    30181 tgaggacaaa gaaatcctcg aagaattgaa agagttatca gaatggcaga aacgaaacca
    30241 agaatatcta aaaaagaagg ctgaagaaga ggcggctcta gctgaggaga aggaaaagga
    30301 aagacaagct cgaatgggag aagaatctga gaagtcagag gacaaacagg accaggagag
    30361 tgaaacagac caggaagatt cagaatcagc taaggaagag tctgaagaaa aagtagcatc
    30421 ctcagaggct gacaaagaga aagaagagaa agaagaacca gagtctaaag agaaggagga
    30481 acaggataaa aagctttcta aaaaggctac aaaggaaaaa ccagccaaag caaagattcc
    30541 tggtatccat atcttgcgag ccttcacgat tttatttcca agtctgcttt tattgattgt
    30601 ctctgcctac ttgctcagtc cttatgcgac catgaaagat attcgtgttg agggaacggt
    30661 gcaaactaca gctgatgata ttcgacaggc ttcaggcatt caggattcgg attatacgat
    30721 taaccttctg ctagacaagg caaaatatga aaagcagatt aagtctaact attgggttga
    30781 atcagctcaa cttgtctatc aatttccaac taagttcact attaaggtca aggaatatga
    30841 tattgtggcc tactatattt ctggtgaaaa tcattatcct attctttcca gtggtcagct
    30901 tgagactagt tctgtgagtc tgaacagttt accagaaact tatttatcag ttctctttaa
    30961 tgatagtgaa caaatcaagg tttttgtctc agaacttgct caaattagcc cagaactcaa
    31021 ggcggctatc caaaaggtgg aattagcccc aagcaaggtg acatccgatt taattcgatt
    31081 gaccatgaat gattcggacg aagtcttggt tcctctatct gaaatgagta agaaactgcc
    31141 atattacagt aagattaagc cacaattgtc agaaccgagt gtggtcgaca tggaagctgg
    31201 aatttacagt tacactgtgg cggataaatt aattatggag gctgaggaaa aagccaaaca
    31261 agaggccaag gaagccgaga aaaaacagga agaagaacag aaaaaacaag aggaagagag
    31321 caatcgaaat caaacgaccc agcgctcatc gcgtcgctag gtttaccttt tctcttatag
    31381 ttctttagtg accatgttct tacgtttaat atttgacatc tgtttttctt tatgttacat
    31441 ttgcaatgta atcgattaca aaataatttt ttatgaagaa ggtaacacat atgtcagatg
    31501 aagcattctt atttgaagaa attgaagaaa tcgtagcccc tacagatggt gaatttttgg
    31561 gggagtttta cttggaactg gggtagttct cttaattgga atagcctgtt gttaaaaaga
    31621 tagggagtga taatcatgca agataacttt ttatttgagg aaattgaaga aatttcagta
    31681 ccagttagtg atttttcagc tggacttgca acaggtatcg gatttggttt agcaatcctt
    31741 gctcttgctg gttgttgaag tttgttcatt tactaacatc aagctttttc aatttcattt
    31801 tagacagtca tttgaatttt ccgtattagt cttgcagcaa gagattaata gaattagtca
    31861 ttattttatt gattgcggac tgagggacta gagtatgttt tacttagccc ctcttttatt
    31921 tattaaaggt taggtttgtt atgagaattg ttgataagat taagatatta cctactcctt
    31981 atgagggaca gtatcatcta tatataccat ccagtaagaa acatgtatta gttgggaaac
    32041 aggaaaaaaa tggttagagc aactaatagg tcaagaattt accatatcgg acttattagt
    32101 gttagtaggg aagaaatatt atttaagatt ttatattgtt cttagtaaaa tgggacttat
    32161 agattctatg aaatcacgaa aaaagataaa tatttttaaa atatcttggg actttaatat
    32221 aacattatct gaaaaattaa actataaaag atttaataag aattttgaaa aaatcctatt
    32281 tggtattttc ccattggctc ttattttgca atgttttatt atgtatattt atagggcgga
    32341 tattttgact aatttccaaa actttccaat aacgctaaac attcttttat tttggttgct
    32401 tgttgttatt atgtctgcaa cgatacatga atttagtcat
//
LOCUS       DAVBCR010000020        31274 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCR010000020 DAVBCR010000000
VERSION     DAVBCR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.020"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            1..373
                     /locus_tag="VQ508_000815"
                     /pseudo
     CDS             1..373
                     /locus_tag="VQ508_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(501..1523)
                     /gene="cbpF"
                     /locus_tag="VQ508_000816"
                     /pseudo
     CDS             complement(501..1523)
                     /gene="cbpF"
                     /locus_tag="VQ508_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1542..2363)
                     /gene="cbpG"
                     /locus_tag="VQ508_000817"
     CDS             complement(1542..2363)
                     /gene="cbpG"
                     /locus_tag="VQ508_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HET2489846.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNRKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2444..2683)
                     /locus_tag="VQ508_000818"
     CDS             complement(2444..2683)
                     /locus_tag="VQ508_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489847.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2833..3099)
                     /locus_tag="VQ508_000819"
                     /pseudo
     CDS             complement(2833..3099)
                     /locus_tag="VQ508_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3124..3435)
                     /locus_tag="VQ508_000820"
     CDS             complement(3124..3435)
                     /locus_tag="VQ508_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2489848.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3360..3602)
                     /locus_tag="VQ508_000821"
                     /pseudo
     CDS             complement(3360..3602)
                     /locus_tag="VQ508_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3603..4235)
                     /locus_tag="VQ508_000822"
     CDS             complement(3603..4235)
                     /locus_tag="VQ508_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2489849.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4249..5244)
                     /locus_tag="VQ508_000823"
     CDS             complement(4249..5244)
                     /locus_tag="VQ508_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2489850.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5241..5939)
                     /locus_tag="VQ508_000824"
     CDS             complement(5241..5939)
                     /locus_tag="VQ508_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2489851.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6017..7027)
                     /locus_tag="VQ508_000825"
     CDS             complement(6017..7027)
                     /locus_tag="VQ508_000825"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2489852.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7011..8018)
                     /locus_tag="VQ508_000826"
     CDS             complement(7011..8018)
                     /locus_tag="VQ508_000826"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2489853.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8005..8958)
                     /gene="mvaD"
                     /locus_tag="VQ508_000827"
     CDS             complement(8005..8958)
                     /gene="mvaD"
                     /locus_tag="VQ508_000827"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2489854.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8940..9818)
                     /gene="mvk"
                     /locus_tag="VQ508_000828"
     CDS             complement(8940..9818)
                     /gene="mvk"
                     /locus_tag="VQ508_000828"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2489855.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9940..10269)
                     /locus_tag="VQ508_000829"
     CDS             complement(9940..10269)
                     /locus_tag="VQ508_000829"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2489856.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            10931..11267
                     /locus_tag="VQ508_000830"
                     /pseudo
     CDS             10931..11267
                     /locus_tag="VQ508_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            11167..11754
                     /locus_tag="VQ508_000831"
     CDS             11167..11754
                     /locus_tag="VQ508_000831"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489857.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            11797..13029
                     /locus_tag="VQ508_000832"
     CDS             11797..13029
                     /locus_tag="VQ508_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2489858.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(13147..14103)
                     /gene="cbpC"
                     /locus_tag="VQ508_000833"
     CDS             complement(13147..14103)
                     /gene="cbpC"
                     /locus_tag="VQ508_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2489859.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(14201..14890)
                     /locus_tag="VQ508_000834"
     CDS             complement(14201..14890)
                     /locus_tag="VQ508_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2489860.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(14902..16326)
                     /gene="gndA"
                     /locus_tag="VQ508_000835"
     CDS             complement(14902..16326)
                     /gene="gndA"
                     /locus_tag="VQ508_000835"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2489861.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(16402..17796)
                     /gene="mapZ"
                     /locus_tag="VQ508_000836"
     CDS             complement(16402..17796)
                     /gene="mapZ"
                     /locus_tag="VQ508_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2489862.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(17809..18966)
                     /locus_tag="VQ508_000837"
     CDS             complement(17809..18966)
                     /locus_tag="VQ508_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2489863.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(19051..19431)
                     /gene="rnpB"
                     /locus_tag="VQ508_000838"
     ncRNA           complement(19051..19431)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ508_000838"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(19452..19781)
                     /gene="gpsB"
                     /locus_tag="VQ508_000839"
     CDS             complement(19452..19781)
                     /gene="gpsB"
                     /locus_tag="VQ508_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2489864.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(19851..20378)
                     /locus_tag="VQ508_000840"
     CDS             complement(19851..20378)
                     /locus_tag="VQ508_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2489865.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            20444..21040
                     /gene="recU"
                     /locus_tag="VQ508_000841"
     CDS             20444..21040
                     /gene="recU"
                     /locus_tag="VQ508_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2489866.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLTVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            21037..23196
                     /locus_tag="VQ508_000842"
     CDS             21037..23196
                     /locus_tag="VQ508_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2489867.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(23363..28666)
                     /locus_tag="VQ508_000843"
     CDS             complement(23363..28666)
                     /locus_tag="VQ508_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2489868.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(28967..30949)
                     /locus_tag="VQ508_000844"
     CDS             complement(28967..30949)
                     /locus_tag="VQ508_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2489869.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         8986 a         7085 c         5592 g         9611 t
ORIGIN      
        1 tctaaaacaa tggaaaagtg ctacacagat gtgacagaat ttgccattcc agcaagtact
       61 caaaagcttt acttatcacc agttttagat ggctttaaca gtgaaattat cgcctataat
      121 ctttcaactt cacccaactt agaacaagta caaacaatgt tggaacaggc attcacagag
      181 aagcactatg gtggagtcct tctttgggat tctgaaatcg gaaatgtttt atggctatga
      241 gaaaacattt aaatcactta atcaattgga acaagctatt atagactata ttgattatta
      301 caacaacaag agaattaagg taaaactaaa aggacttagt cctgtgcagt acagaactaa
      361 atcctttgga taaattattt gtctaacttt tggggggcag tacagaaaaa ccttgatact
      421 atgcgttttt tgattgtgaa gacttttatc cacctattat acaaagttaa gtatccatca
      481 cagtttacaa ttagccttca ttacttaacc cattcaccat tatagtttaa gtagtaacca
      541 cctactgtgg tattaacagc taaagcacct gaatcatagg catagtacca gttaccattg
      601 acttggaacc agcctgtttt catatctcca ttacttgcat ttagatagta ccaagtcgaa
      661 ccttcctgat accaaccagt tgccatagct cctgatgaac ggagatagta ccacttgtta
      721 ccaaggtatt gccagccagt tctcatatca ccattttcag catctagata gtaccaagtt
      781 gagccttcct tataccagcc agttgccata gctcctgacg aatggaggta gtaccatcta
      841 ttacctagaa attgccaacc agtttgcata atgccagttg ttggatctag atagtaccat
      901 ggagctgctt ttagcttctc ttcatcatac gtaagagggg aatccttaac ccaaccacat
      961 gctagctctc caaccgccaa tctgttgatg cgcgaatcaa agccaccatc cttctgtaaa
     1021 taataccaat gaccctcttc gtaaatccaa ccagttttta aagtatgata actacgctga
     1081 tcttcaaaat aatagactca tttctctgct gggctatcat attcttcccc atgatgtttg
     1141 ttggtattcg tagcagtttt tgcttctaaa acttgcttgc caacaaattc ttgtaataca
     1201 ccatcttgac caaaataaaa ccaatctggt ctaagagaaa tctctattct tggagaagga
     1261 ccaatcgtaa cccccttgtg tggagcaggt atatattgcc agccgacaac catctctccg
     1321 gataaaggat caaaataata gtacttccca tctatcactc tccagtaggt ttcttttaga
     1381 tccccctttt tgtagtaggt tctaccattt tcttggacaa actgccatcc ttcagaatca
     1441 tctgcaaata ctgtactggt ccctagcaaa ccgaagaaaa atgtggctag tgcgacttgc
     1501 atcatttttt tcaaaagctt cattttatat atcctccaac attaaatcca ctcaccagat
     1561 gaagcgaagt tataaacttt accatcgata gtttggctac ctgtaaccat tgctccattt
     1621 gaattgagat aataccattt tctatttact ttttgccaat ctgtaagcat cttaccggaa
     1681 ctgtctaaat aataccaagt atcatttatc tcctgccaac ccgtttgttt atcatgttgt
     1741 ctataatagt accaactacc atttattttc ttccatcctt caagagagta acctttaaga
     1801 accgaataaa taaatggcaa atttcgttca tttaatttaa ctgcactgtt aatttgatta
     1861 gctccatctc ctaaagtatg cactcctact acacggtgac tagcatcata aactgtagat
     1921 ccactagacc cctctaaagt atcaacttgg taatccaaga acatgccatc atcacttaaa
     1981 acttgttttt tatctgtata catttgatga attttaaaat tatatgatgg atagcctgtg
     2041 atagtcacag ttattcctgt caaatttttt tgactagtag gaagacccaa agtccctaat
     2101 tttgcaccaa tgggctcttc tagaattaat aaagccaagt catattccct tgcatcctta
     2161 gaatttaaat ttctaaattc cttcaaataa cgaacttcct ttactttgat ctttccaaat
     2221 agttcttgac ttggactaac agccggaaga acataaatat catccgcttc tttcccatag
     2281 tcatgtctgt agtagttgtg agccgctgta attaacacat tatctttaat aaaattcgca
     2341 cttcccctcc cttctacatt cattccatcg gctactccat aatacttaga taaaaccata
     2401 gctgaagtcg aatacggata ctgtaaagta ttatcaattt taatcaaatc atcattaccg
     2461 ataatacttc tgattgcttt tggtagtatg aaccatacgt tggtgaaatc tcagataatg
     2521 aagaatcatt agactctgga cctttttcta gtgtctcact tacctcatat tcttcacctg
     2581 tactagaaat aacactcaaa gcagatactg tcgataactg gctagccaat aaagtactcg
     2641 caataattga aatacccaat tttttataaa cagttttctt cattattgta tcctcctaat
     2701 gtaattatag cgtactattc taaatttctt aatctactat agaatcaaga aatctaccac
     2761 cttctttaaa taccctccat tatcacataa acaggtaaac ttttcaatta atgactgcgc
     2821 ttttcaatca cgctagaggt acttacttgc ttctttgata ctaagttcag ccattctttc
     2881 cttgttttca tcaataaagc atgttaccca agttggattc gttttggagt agtctcgcag
     2941 aatccagcca atggctttat tgataaaaaa ttctgtttgg ttcaagttat gaagtagaat
     3001 cttttccatt aattgagtat tggtcttctc ttttcttaac aactggtggt caatagcgac
     3061 acgtctcagc cagatattat ctgataggct ccattttata ctcaatgaaa atcaaagagc
     3121 aaactaggaa gctagccgca gattgctcaa aacactgttt tgaggttgca gatagagctg
     3181 acgtggtttg aagagatttt cgaagagtat taagattatt tcttctagtt cagggtgttc
     3241 atacaccaaa ctccctacta ctcgatctag gatatctacc gtgtcccacc aggattttgt
     3301 cacgactaac tgctctagct taggcaaatc ggtttccttt agataagact gcattgcttt
     3361 caaatagtta gcagccacat attggtattt tctaggctcc ttttcccagc aagtgtctgc
     3421 aaaatcccaa tcgataatct ttgttttttt cgcttctagt aaatattttt atagagttta
     3481 tttctttcag gcaccgcaat acctagaaaa gaaaattgat ggcgcatata ggcttccatg
     3541 ggccttgctt ttttagagtc ttttgctgct tctagctcct caagtaaatc tgctaaactc
     3601 atctaaaact cctcttgccc caccaaatgg tgctgaaagg catagacagc cgcctgggta
     3661 cgatcgctga cttcaagttt ggcaagaata ttggacacgt gggtcttgac cgtcttgaga
     3721 gagataaaga ggtcatctgc gatgcgctga ttttcgtagc ccttggcgat gagttggaga
     3781 acatctcgct cacgcgcagt caattcttca tgaagttcca tatgattgcg gtggtattca
     3841 accttcttgc taacctcttg ctcaatggcc agctcgccag cagctacctt actgacggca
     3901 tgaagcaatt catctgcact agaagtcttg agcatatagc ctttggcacc agcatctaag
     3961 actggcatga ttttttcatt gtccaaataa gaggtcacaa tcaaaatctt ggcttcaggc
     4021 cattctttaa ggattgctaa ggtcgcgtca attccattca tctcaggcat gacaatatcc
     4081 atgacaatga catctggacg cagttccaag gccaagtcaa tcccttgaga cccgttggac
     4141 gcctcaccca caacttctac atcgtcttgg aggtcaaagt agcttttcaa gcccaatcgg
     4201 accatttcat ggtcatctac tagtaaaatt ttcatcttta ctcctttatc attccttatc
     4261 taacagggga atacggatat caaccgccag cccttgcttg ggagctgtca agagttgaac
     4321 tgttccagcc atatcttcaa cccgctcctt gatatttcgc agtccataac tcaagtcgtc
     4381 taagctccct aactggaaac caatcccatt gtccaccacc ttcagttgca attcaacatc
     4441 tgtctgatag aggtagacat ctaggcaaga tgcctgggca tggcggaggg tattgctaat
     4501 caactcttgc aggatacgga agatatgctc ctcgattttc ttaggcaatt tcgtcatatt
     4561 ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc tcttttaaaa gaatttgaat
     4621 cccttctatc aagctcttct gctccagttc aactggtcgc aaatgcaaga gcaaaacccg
     4681 caaatccttc tgggctgttt ctaaaatagc tgtgacactc tgcaactggg tctgcatctt
     4741 ttctctatcc aatttcaaag cctgctgact gatacccgat aaaatcatgt gggccgcaaa
     4801 caactcctga ctgactgtat cgtgcaaatc ccgagcaatt cgcttccgtt ccttctcgat
     4861 gatttcctct tcctgagcaa ggctatgatt ttcagctttt tgaagagcct ctgtcaaaag
     4921 gttaagttta cctgataagg acttgaaact ggcatccaaa tctggatcta caacctgaac
     4981 cacttcttgc cctgccaata aacgcttgag attagcctgc atttttctta gagaaagctc
     5041 ttcgatccct cgccaaaaca gggctaagag acaggtcatg gacatgctga aaaccaacaa
     5101 taaaaagaaa aatttttctg ttttttcgac atcgtgcaaa aagatagacc agtcaaaatc
     5161 aagtatttcc agcaagctat gggagaaaaa aaagacaaac aggaaggagg tgagagcgat
     5221 aatgacatag gcttgttttt tcatcctcta accacctcaa catcaccaat catagtggtc
     5281 aagaaaatct tgacactctt gttactcttg agatagtctt ttgtttcttg atgatagtgt
     5341 tcattgcgga gggctcgctt gggctggttg aaaaaagtca aatccccata gagacagtta
     5401 acgctgagac tgacttccac atctacaggt acgatgattt tggtcgttcc taccatcttt
     5461 ctgaggataa tgacattgtc atgattggtt aagatgactc tctccagatg aatagtgtcc
     5521 ttgcccatga agcgaaagag attgatatca tcaaattggc aagtctggta gcttgaaaaa
     5581 tgatgaagat ttccaaacca acgatttttc tccttcttaa ccgtcacaac ctcttcaaaa
     5641 accaaattgg tctgctcttt ttcctggttc atcatcgggt aaagaagaaa gagactatag
     5701 ataaccgcaa caaaaatagc tagaatcaca aaaggattga gcataacgat gaaaaagaag
     5761 agaatggttg ccgctactaa aagaagatta ttcccctctt taccagtata gtagcgaatc
     5821 aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa aatgctctga taccatcaaa
     5881 atcagagctc ctgtcagaag acaggcttcg ataaataaaa agattttaaa ttttctcata
     5941 ggttcatcct ctcccttcta ttttatcaca attcaaaaaa gtcacctcag tctgaggatg
     6001 gaaaaaaagg cgctggttac gcctttttca tctgatcctt tgcttctttt aattttccat
     6061 aaagaagata gtctactttt tgtagatctg ctatggtggc acagttaagg gaacacataa
     6121 tcaagcgtag atctgctttc cagccttgga caatgccaat cacttcttca actgtgtagg
     6181 tttcaaccaa ttccagaacg gttcgtgaca atcccacagc cttagcacca aaaaccaagc
     6241 acttaatcat atccagcgga ttccgaaccc ctccactaac caagagttcg accttatctt
     6301 tccattcttg ggcattgaga agggcctgca tggtagactg accccattga ttgaggtaat
     6361 cacgctggcc actacgacgg ttttcgatat aggcaaagct ggtgccacca cgacccgata
     6421 ggtccactgt acgaacaccg aattcatagg ctctttcgat tgtcttggca tccattccaa
     6481 agcccacttc cttgaggaca ataggaacgg gaatttgctt gctataatct gctagatgcg
     6541 attgccagct tctaaacttc ctttctccct cgggcatgag taattcctgc atgacattga
     6601 catgcacttg caatagaaca ggattcatct cttctacagt ctgaagtcct aactcgacag
     6661 gtttgtccaa tccaatattg gttccaagga ggagattggg atgactagac ttgacagaaa
     6721 aagaatcatc cgttggattt ttgagggctg cgctataaga acccgttaca aataaaatac
     6781 cacaggattc cgccacctga gccagctttt gattgatttc tcttccctta ttacttccac
     6841 cagtcatggc attgatataa aaaggaaagt cccactttcg accagcaaac tctgtcgaaa
     6901 gatcgatttc atccagattg taaagaggca aggaagaatg aatcagctcc acctcatcaa
     6961 agctattata ggaacttttc tgctcaaggg catagaggat atgctcgtcc ttacgatttg
     7021 tcgtcatgtc ctatcctttc ttgatataag agctcaatcc ccagatcggc ccaacgattt
     7081 tttaaggttt tggttgattg cgcatcaaaa ctcagggcga tgccacagtc accaccacca
     7141 gcaccactac tcttggcaac ggcctgcaaa tcttgactgg cttctttcaa ctgtctaagc
     7201 aaaggcgtgt aaatatctgt actcaagcct tctaaaagct tgctggctac ttctacttgc
     7261 tcgataatct tttctgattt cccctgttcc aaggcttcga ccaaagaaac caccgtttct
     7321 tttgaggaac ttaaaaaatt ttgattgata ttttgcttga tttgctggac catgtgactc
     7381 gatacagcca cttccttggt ccatcccact aagaaatcac attctaaagt tggtttcact
     7441 tgtgaaattg aaaagcccca atcacgctcc agaactgtcg ccaagttttc ttcttctaac
     7501 caagcagcca ccttctggcg atcaaatgac tggtagagaa ccaaatcctc tgccacaata
     7561 caggcaaggt cgcccatgga accattgtct cctcgcttga gcaagacagc gctagtcagc
     7621 ttgaacaaga gctcctgatc aacagaaaca tcatacagag ccagtaaagc cttgacaacc
     7681 aagacaacga cgctgccact agaacctaga ccaaactttt tcccttctcg ttccattttg
     7741 ccacagattt ctagggaaaa agatcttaaa ttctgaccgc gaacagcgag gaagtctccc
     7801 atcaaagcaa tcgtttcttg aatcaagctg tagtcaggat tgggcgttaa gtccactgcg
     7861 aaatcaaaca tatctgaata gatacggtag ctgtcagaaa aagcaatctc agccctcata
     7921 tagatgggaa tatcctttat caaagctaac tgccctggct ctaaaatagc atattcacct
     7981 gcccaataga gttttccgca agttttaaca gcaatcatct tgactcaaat cctttgtttt
     8041 tgacacaatc aagcgataac gctgaccgaa aatttctgac aaatgctcca agtctttctc
     8101 ctgacaaaag accttaacat tgggaccagc atccatggta aagtagcagg cctctccttt
     8161 ctcacgaagc tggcgaacaa agtccatagc ctcataagag gcatccgtca gataagaaaa
     8221 ggctggacta gcagtctttg tcgtagcatg catagccagg gcatttttct ccgttaattc
     8281 tccaatcttg gcaaaatcat tttccttgag ataaatcagc atatcctgat agtccttctc
     8341 agactgacga acccagtcgt caaaagtcgt cgaggtttcc acacaaagtt tcatcccgtc
     8401 acggctagag attggttttt tcttgtcctc tagcaccaac ataatcatag ctagtttcaa
     8461 gtctgtctct acagagtaaa tttctccact atccttatcc caggctccta gcggtccata
     8521 aaaactccga gaagaagagc ctgaggcaaa cttggcttcc tgcgctaact gacttctatc
     8581 caatccaagc ttgaaataag cattacaagc cttgaccagg gcggacaaac cactagaact
     8641 tgaggacagg cccgctgcag taggcatatt gttttgagta tcgatacgga caaagccctc
     8701 accagctgga cgataacggt caataatctt actcatcttg gcatgctcga cctcattttg
     8761 tagctgacca ttgatgtaaa attcgtcagc tattacattg gctggtaaag gcgacaaggt
     8821 cgtctctgta tacatatttt ccaaagttag agaaatactg ctagtagcag gcaccatctc
     8881 tttttctttt ttctttcccc aatatttgat aatagcaata tttgcgtagg aacgtactgt
     8941 cacaggctct ctatccatgt ctgaacagct cctttctctt ctaatctttc tgctagttct
     9001 tgtgcgtgtg tcaaattggt taccaaggct atgatacaac ctcctagccc accaccgctc
     9061 atcttggcac ccagagcacc atggctaaga gtcgtttcaa ccaaaaagtc tgcctcaggg
     9121 ctactgactc caatttcttt taaatgtaaa tgcgcttgac tgaggatttg tcccagtcct
     9181 tcagcatctt tttgtgaaat cgcaacttct gcttgctggg ttaattctcc caaggcatgc
     9241 aaaaacggta gggcatcctt gcccttattt tgaaccactt ggatggcttc acgagtatga
     9301 ccataaacac ccgtatcggc aatcaccaaa taggcggata aatccatctc aagttctgta
     9361 aatcctacgt tcttgataaa gcgaataggt tggtcactga gacaggtctt agcatccaaa
     9421 ccactaggat tcatatgggc aatcatctca gctcgattga ccaagatttc tagtacatca
     9481 tgaggcagat cagcctgata gtagtcaaat accgcacgaa tggccgctat gctgatagcc
     9541 gctgacgaac ccatcccccg tttctcaggg atagccgagt caatctcaca acgaatgcag
     9601 gcttctgtga tatccaaata ctccagtgag gcataaaccg ccatggacaa ggtatcctcc
     9661 tcataaaggc gccaaggact ctctgcagga actaccttac aggtcacctc cacctccaaa
     9721 agaggcaggg aaatggcagg ataaccgtaa acgaccgcat gttcccctat taaaattatc
     9781 ttactatgtg cctgaccgac accaactttt tttgtcattt tttcctttta ctagacgaaa
     9841 aaacgtctta tttttcatac aagtattaat tctttcctat ctattttatt atattttcac
     9901 aaaaaaagcg attgtttcca ttcacaatcg cttctttcat tattgaaccc attcgccatt
     9961 atagttgaca gaatagccat ctacggtcgt attcactgcc aaggcacctg agctataagc
    10021 gtagtaccat ctgccattga cctggaacca acctgtcgtc atagaacgac gaaagaaact
    10081 ccataccgtt aagtaaagag gaaagtcgtg agggatcatg cgccattgac aacctgtttt
    10141 agtgacgtac aaagtctcat taacaagtac tcgtttcggc catttatagg tgcggtgttt
    10201 ggagaaatag ggttcaatct tagcccattc ttgatcgttt aaatcagtat catatgcttt
    10261 gcgtgtcata actctagttt aacatttttt gtgaatacaa gttctaaata atcgaccacg
    10321 aaaatttctt aagtggaaaa cgccttatga agtatgctac gggaaactta tgcacttaat
    10381 ttgacaattc aagatgtaaa aatatatact atagtagatt gaaactagaa tagtacacct
    10441 ctacttctaa aacattttta gaaatcgatt tgactgtcct gatcgatttg tcctgttatt
    10501 attttatttt actataatat ttgacaagtt atcctaaaag tattattatg ttgttgtgtt
    10561 atagattgat tgaatctaac taaaggatcc tattcaatta ctagaactat cacatactca
    10621 aggtcagctc acagatgagc aactattttg gttacaatgt ctactaaatt taagtcaaac
    10681 aaataattta gtcaaaatta aaaaaataga ggaacataaa tatgattaca aaacagaatg
    10741 taatagtgtt ctacgatttt tactagataa aactgtaaat tctgaaggaa ggatcacttc
    10801 ttcaacagaa tttggaaatt tcgtaagtaa tttatcattc caacacggaa tagctggact
    10861 actgtttcct ctaaataaat tgtacccccc agaactggat tctaaaatac tctctatcat
    10921 caagaaggca gtgacaatta gaacgacaca catatgaata tcaatactca ctgctatttg
    10981 gtgatgcagg ctatctatgg ttactcctac atttattttc tatcagtaaa aatcaatact
    11041 atctacaatt agcaaacgtc accgctaaga aattaataga gaattatgat actctagaag
    11101 aaatagactt tgcattggga aaatctggtg tcctattatc attaataaaa tactatcaat
    11161 ttaccaatga caatactctt aaaactttca tccacaatag tatatgggga aatttatcat
    11221 tatttcctac aaagagatac agccaaagaa agcattttag actatagctt tgctcatgga
    11281 tattgtgaaa ttgcatatgc tttatttgcc tattctaaag tcttagaacc ttctatgttc
    11341 tataatgatc tccatacatt ccatgctgaa ttaaaaaaat tattagaaaa agttacttct
    11401 aatactgaaa atttaggaaa tttacaactt tcttggtgcg aaggaatttc cggaataatc
    11461 ttatatcttt gtatgtacga ttgtgacgga aacaaagata ttattagtaa atatcaagaa
    11521 tttgttttta accatcatct aaaaatgatg acaggatatt gccacggaat aactagctta
    11581 ctacaaacca ctgtctacaa tcaaaacaaa ttactgatga aaaaaatcca acaggtaatt
    11641 ttagcatgtt ctgaacgaga tgatcacggt ttactgatgt ttcaaggaga tagtggtaaa
    11701 gcagatttgt ttgacttcgg aataggaagc agggggtata ttggtgtcta ttaaataata
    11761 aattcccatt cgatgtgcaa acataaggag aaatgtatga aattattttg gacaaacaac
    11821 atatatagac agttgctgct aaacagctgt ttttcatcat tcggcgacag tattttctac
    11881 ctcgccatta tcaattatgt ggctcagtac aatttcgctc cgctagcgat tttactgatt
    11941 tccatttcag agatggttcc cctactatcg caactctttc tcgggattct aggagatttt
    12001 caagaaaata gagtcaaaca cgcactctgt attgccaaaa tcaaaatcct gctctacgct
    12061 attttgacag tatttctcgt cttgtcgccc ttttcattag tttcagtcat tatgattgtc
    12121 atcatcaacc tcatctctga caccttgagc tacctgtctg cctacatgat gaacgccctc
    12181 tacatcagtg taattaagga tgacctgcat gatgccatgg ggttcaggca gtctctgatg
    12241 agggttgtcc acattgtcgc caatctggct ggcgcattcc ttatcaatgt tataagtatt
    12301 caaactattt cccttatcaa cactctgact tttgtcattg cctttttggg cctgtatgtt
    12361 attcgacata ccttgtatga ggttgaaaaa aggattgaaa tgtcacatac agcactgagt
    12421 tttaagaaat attttcaaca tcttaaacag tcgctggctg tgctcctgag gttaaaagat
    12481 accgtcatac tactgtttct gacgaccagt atgattgcca tcttggatgt gtcccctcgg
    12541 ctgattgccc tccgcttcat ccaacagaca ctagcacaac tgagcattgg gcaactcttc
    12601 gccctgctct ccatcatcat gtcttgtgga gctatccttg gcaatatgac cagcagtaat
    12661 ctatttaaaa atatccgttt cacgcacctc ttggttttct gtgagatttc cctattggct
    12721 ctaataacta gtatcctttg tcaagcctat atcgtaattt tcgtgaccag tttcatcagt
    12781 tctacgatta tcggcattct cagccctcgc ctacaagcag ctgtctttgc ccatatcccc
    12841 agtgacaaga tggggacggt gggctctgct ctgagcacag tagacattct cgccccgtcc
    12901 ctgctctccc tattagccct atccatagca tcgggcgttt cggtgcagtt agcattgata
    12961 tttttgtatc ttattttgat tgctcttatc ttttgtcaat ggttagtcaa gttcaacact
    13021 cataactaac gaaaaagcat gtgtagattt cacatgcttt taatctcccc aatcgtcagg
    13081 tcaagtacaa caaagtcact tctttgatta agcgagtgtt ctaatataat tataagcgcc
    13141 ctgtcattac cgaacccatt cgccattata gttgacagaa tagccatcta cggtcgtatt
    13201 cactgccaaa gcacctgagc tataagcata gtaccagttg ccattgacct ggaaccaacc
    13261 tgtcttcatg tctccattac ctgcatttag gtagtaccaa gttgaaccat cttgatacca
    13321 accagttgcc atagctcctg atgaacggag atagtaccat ttgttcccaa ggttttgcca
    13381 acctgttttc atatcgccat ttggctggtc taaataatac caagtggtac cttcctgata
    13441 ccagccagtg gccattgctc ctgaggaacg gaggtagtac catggagcag gtttgcttct
    13501 atcaatgtca atagtaacat gaaaatcttg agtccaaccc ttagcaactt caccaattgg
    13561 tagtggattg taagaatcat caccaaaatt tcctagctta tttaaataat accaattgcc
    13621 gtcttcataa atccagcctg tctttacagc ataattatta tcaaaataat aagttctctt
    13681 ttcagcagat agaggatatt gttcaccgta tacttttcct gtattttcag acgtttttgc
    13741 ctctagtact tgtttatctg tttgttctag caaagcacca tcttgtccaa aatagtacca
    13801 cttctcctga aggccaattt cgaagactgg acgattatca aataaatcat cacgataacc
    13861 tgttccagga atttctaagt attgccaacc gacaaccatc tctccaatat caccaaaata
    13921 gtaagtttta ccgtctattt tatgccaata gattgcttta tgatcatctt ttacataata
    13981 ttttctatta tccttatcaa caaatcggcc acctgtggta ttcgcaaata ccgtatttgt
    14041 agctagcaaa ccaaagaaaa agactgctag tagaacttgc atcatttttt tcaaaagttt
    14101 catttatata tcctcctaaa acgattgtag caaagacacg actgcatgtc aatatataaa
    14161 aactcctcaa aaaagcagtt acacaactgc aactgctttt ctattcttgc atggtgtaac
    14221 caacaccacg cacggtttta atgtagcttt tttgtccttt aacatcaagc ttgctccgta
    14281 gatagcggat atagacatct acgatatttg tctcggtcgc actttcatac ttccaaacac
    14341 tttccaacaa ttgctcacga gtcaatactt tcttgcttcc cataagtgtc gccaaaaggt
    14401 catactcacg gcgggtcaga gcaatcatct cttcaccacg ataaaccgtg tgatgttcaa
    14461 catctatcct aagattgcgg taggtccttg gaactttcat cagactgcag tgttgatcaa
    14521 tgaagtcccg acctcggaag atcgccgaaa tacgcgctac cagattttcg ataaagactg
    14581 gcttatagat gtatgaaact gcaaaacgct gaacaacttc cagctcttct tgcaagtctt
    14641 cccaatgatc taaaatcatg atgactgagg caggtttagt tcgactcaat ttttctgcaa
    14701 aatcctgagc catcatatct cccagattaa cgttcaataa aatcaaatca tagtctgtct
    14761 gaagagccat ggaaagggct ttttgcccct cctctaccag atcaacccga tactgctctt
    14821 tctggagttc caaacttaaa aaatgagcta gatttcgttc tttctcaagt aataaaatcc
    14881 gtttccccat ggctgaccta cttatttttc gtcataccaa gagtagtgga aggttccttc
    14941 tttgtcttta cgttggtaag tgtgagcacc aaagtagtca cgttgtgctt ggatcaagtt
    15001 agccggaagg tcagctgaac ggtagctatc aaagtaagta atagctgctg agaaagttgg
    15061 cactggcaca ccagcttgaa ccgcaagagc tacgatatca cgcactgctt gttgatactt
    15121 agcagtaaca tccaagaagt actcgtccaa aagaaggttg gcaagatctg catcgcggtt
    15181 gtaagcatct gtaatctttt gcaagaaacg agaacggatg atacagccat cacgccagat
    15241 agatgcgata tctgcaaatg gcaagttcca gttgttttct ttagaggcta cacgcaattg
    15301 agcaaatcct tgtgcgtatg aaatgatttt tgagaagtaa agggcttgac ggattttttc
    15361 aatcaattca gccttgtctc cttcaaagtt gaaggcagct ggttttggaa gcaccttgct
    15421 agcatgtaca cgttcttctt tgtaagttga aatgtagcgt gcaaacactg actcagtaat
    15481 cagtgacaat ggtacaccaa ggtcaagaga tgattggctc gtccatttac cagttccctt
    15541 gttacctgca gcatcaagga tgtagtctac gattggtcca tcttggtctt catcgtcttt
    15601 acggctcaag atatcagctg tgatttcgat caagtagcta tccaattcac ccttgttcca
    15661 ctcagtaaag atttcagcca tatcctctgc agaaaggcct agcaagtgtt gcatcaagtc
    15721 atagctttct gcgatcaatt gcatatcacc gtactcgata ccgttgtgaa ccattttcac
    15781 atagtgacca gctccatcag gaccgatata agtcacacat ggtttgccat cttctggtgc
    15841 tttagctgag atttcttcaa gaacatccga aaccaattcg taggcctctt tttgtccacc
    15901 aggcatgata gaaggacctt caagggcacc tttttcacca ccagaaactc cagtaccgat
    15961 aaagttgata cctgagtttg ccaattcttc attacgacgg atggtatctt tgtagaaagt
    16021 gtttcctccg tcaatcaaga tatcaccttt gtcaaggtgt ggaagaaggg cttggatagt
    16081 agcatctgta ccaggtccag cttgaaccat cagcatgata cgacgaggtt tttcgattga
    16141 gtttacaaaa ctttcaacgt catagcttgg tacaaaattc ttttcaggat ggcaagcaat
    16201 cacatcttcc gttttttctt tactacggtt gtagatagca actgtgtaac cacgtgattc
    16261 aatattaagg gcaaggttac gacccattac ggccatacct acgacaccaa agttagcttt
    16321 tgtcatttga cactcctctt gtttcatttg ttttatttta tcatttttac ttttgaaaag
    16381 aaagaatttt gtaacgactg cttagtagtc caagtcatcc gcatgaccag cgccatttcc
    16441 gacaaagtag cctgtcttac agttaagggt aaagagatag gttccatctg gcttatagag
    16501 gttgtaataa ccattgccgt taacgatatt gacacgttca aggatatatt ggtctccagt
    16561 gatatagcca cgtgaacgtg aagttgctag aatttgttcc aaaacaccat ccgcaaaatc
    16621 ccaggcagag ttattactat catcaatagc agactgatta taggcaacac gactggcact
    16681 tctttgaaca gcaacccctg cactcgagag acccatattg acttcactgc gactacttct
    16741 agtctcattt gaactacttg gttttggctc actagtcgta tttgaacttg cttgacttga
    16801 acttgagctg ctagtttgac ttgaacttga gctagaagta cttgtttgct ggctcttacc
    16861 aagactgata gccttatcta gcactttatc aagctccgta tttccagttt taatatccgt
    16921 aaatttagca tccgatttgg ctttggcatt ggtatccaac acaccatcca caatagctgg
    16981 tttttcaaat tgtgcattga catcttgaat ggccttgatt tgcgttacta gactatcata
    17041 tttagatttg gcaagcgtat gttcacgact accttccagc ttatcaagta aagtcttgag
    17101 ttgactcagt ttatcaaact ggctattttt caaagccgtt ttattgctat ttgtgtaaaa
    17161 ggcgtcataa agtgtattaa aatcatccac atccgattga ttggctgtag ttgattgaga
    17221 agtttcaatt tccttggtcg aacgagccac ttgacgatag acataataag cactgacaca
    17281 aataattact gatacaagcg ccaaaacggt caaaataaag ccttttttac ttttcttttc
    17341 tgagtcctct tgatccagac gagaaaggtt ttgctcctct tccacttctt cagtcatttc
    17401 tgttggattt tcatcatcta gcaatagagg atccaagcct tgctcctcat cgtccagagg
    17461 caagggtagt aagatgtctt cagaagatgg aacttcctcc gaagaagctt ctgacttctc
    17521 tacagcctca cgcatctctt gaattaaatc atccagactc tgagtttcaa cgaactcctc
    17581 ttttttgtat tgacgagtcg caaacttatc cgcctcaatt tcatctctgt gttgcttaac
    17641 atacttgtcc aaaatggaat cctcaggcaa gactcctgct tccacttctt catttttacg
    17701 aatagcttga ccaactgtta gctcttttgc ttcatcaaaa tcaaattgcg gttcttgacc
    17761 ttctttttta tgacgatttc gtcttttttt actcatgagt atccctttct attttacctc
    17821 ttgccgtttg acacgctgac caaaatattg atatagatcc actttcaagg ttccgttgta
    17881 taacttacgc ttcttatctg cttggctacc atacttgctt tcaaaagctt catcactagt
    17941 caggataaat ttgctccaag ttttcagcgg tgcaaatact tgccccatct cagcatagag
    18001 cttggtcact cctgcatcat ctgacaaacg ttcaccataa ggcggattgg aaatgattac
    18061 tccattgatt ttatcggaac gtaaatcctg cacgcgcatc tgcttaaaag taatatctcc
    18121 tgcaacacca gctgcctgag cattggcctt agcaatttcc accatgcgag catcaatatc
    18181 acagcccatg atatccaact caagcttacg ttctactttt ttagccgctt ctgtgcgcac
    18241 ttcttgaatc aagcgatcgc tgatccagtt ccattcctca aatgcaaaag agcgacgaag
    18301 acctggcgcc atctttctag caatcataac tgcctcaata cagaaagtcc ccgaaccaca
    18361 ggtcggatca atcaaaggct tgtctggata ccagttagaa agttgtaaaa tggctgctgc
    18421 catattttcc ttgataggag cgccaccttt ttcggtacga taaccacgtt taaagaggct
    18481 agacccggtc gtatcaatca tgacagttgc cacatctttg agaatagaga cctcaatctt
    18541 aaactctggg ccattctcca tcagaggaac cccttctggg cgagcatagt gtttctgcaa
    18601 tttcttgaca acagctttct tagaaatagc ctgaacactg ggctcattgt gaagtttgga
    18661 cttaacacat ttagcttttg aaatcggtaa ccgagctcca agtggtaaat aattttccca
    18721 atctaaagcg aaaactccct gaaatagctc ttcaaaagtc ttagctggga acgttcctac
    18781 gataattttg atacgatctg ctgcccgaag ccaaaggttg gtttcgataa tagctctcac
    18841 gtctccttga aaacgaacac gtccattttc aacctgacaa tcgtagccca actctcgcac
    18901 ttcacgacca acgacagcct caagccctgc tgccacagtt gcaattaaat taaattcttt
    18961 tttcatgtta cagtcttgca agacctttct atatgtccac tttaaaaaat gaggttgaga
    19021 aaatttctca gccccaacct atatgcaatt ttctataagc catgttttgt tccagaagtg
    19081 actaggacca cttccttcga taatcatctg tctaccacta acagctctag ctgatagcag
    19141 tcagaatact acgttcgttt ccgcatactc catgccccga ccaaaatttg ggttgctagc
    19201 ttgaggggtt taccgcgttc cactctctct gtttccagaa agactccgtc actgtggcac
    19261 tttcaagcct actctggcct atccaaggac ttagccattt caactgccgt aacgatttct
    19321 cgtccctagg cttatggttt cgcctagcac aaacactaca ggcatcacag cctgtgctag
    19381 catggacttt cctcatgaga agcaattctc ctcacgcgat tatccaaaaa ttgcacatct
    19441 caaataacta cttaaaaatc tgagttatct aaaatttgtt taccaaaaac ttctttttcc
    19501 aatctattca ggcgtttcaa aatatcaaaa ttcgtcatag aacttgtaat tgccgcttca
    19561 aggggttctg cttgaactgg tgaaggtttc ggtttacgag ttaattcttc cttcaaatcc
    19621 gcaatttcct gacgaagtga cttgaccaag gcagcatagg tttcatagtc cttgatgaca
    19681 tcgtctaaaa actcgtcaac ttctacttta ttatagccac ggacttcacg tccaaactct
    19741 tgttcaaaaa tatctttcgc tgaaaaaata atacttgcca tgtctctctc cattctcgct
    19801 tgctagtatt attatataaa aaaaggcaaa caaaaatcaa ggtcaaagct tcaattttcg
    19861 gaaaaatttt cagcaagttc atttaattga tcaaatgtta atctctttat aaaatagtcc
    19921 tcttgatttt tcatcttttg gtaaaaataa gctagttttg tttcattttc ttcatcataa
    19981 aaaagatagg aactagtcgt gttttccagc aaaaactgct ggtaatctct taactgcccc
    20041 ttgtgttcat agcgaggata agcatatttg acaaagtcta cctgcttaaa gcgactcagt
    20101 ttcatctgat tgccttcatt ccaattttcc ccatgggttt caaaagcaaa aatggtggcc
    20161 aactggaaac cgtattcggt tttcatctcc tgagcaacct ctaaaaccca atattcaaaa
    20221 cccaaactcc ctgtaaacac aagccaagac accccatctg ctgccatagc ctctaaatct
    20281 ttacgtatcg cttttttaat caatttaaga cgaggatcct tatcagaaaa taaacccaaa
    20341 tcaaaagcag aatagcctaa aaccaaagct gtagccattt ttaacccttt ctgtgcaaat
    20401 tatggtataa taaagtgatg ttattgtacc aataaggaga attatgatca actatccaca
    20461 taaagtttca tcacaaaaaa gacaaacatc tctttctcaa cccaaaaatt tcgcaaatcg
    20521 aggaatgtct tttgaaaaga tgatcaatgc taccaacgac tactatttgt ctcagggctt
    20581 gactgttata cataagaaac caactcctat tcaaatcgta caagtggact atccacaacg
    20641 aagtcgtgcc aagattgttg aagcctattt tcgacaagct tcaacgacgg actattctgg
    20701 cgtttataat ggatattaca tcgactttga agtcaaggaa acaaaacaaa aacgtgcgat
    20761 tccgatgaaa aattttcatc cacatcagat tcagcatatg gaacaagtcc ttgcccaaca
    20821 aggaatctgc tttgtccttc ttcacttttc ttctcagcaa gaaacctact tattgccggc
    20881 attcgatttg attcgcttct atcatcaaga taagggacaa aaatcaatgc cacttgaata
    20941 tattcgagaa tatggatatg aaatcaaggc tggtgccttc cctcaaattc cttatctcaa
    21001 tgttatcaaa gaacatttat taggtggtaa aacaagatga acaaaccaac gattctgcgc
    21061 ctaatcaagt atctgagcat tagcttctta agcttggtta tcgcagccat tgtcttaggc
    21121 ggaggagttt ttttctacta cgttagcaag gctcctagcc tatccgagag taaactagtt
    21181 gcaacaactt ctagtaaaat ctacgacaat aaaaatcaac tcattgctga cttgggttct
    21241 gaacgccgcg tcaatgccca agctaatgat attcccacag atttggttaa agcaatcgtt
    21301 tctatcgaag accatcgctt cttcgaccac agggggattg ataccatccg tatcctggga
    21361 gctttcttgc gcaatctgca aagcaattcc ctccaaggtg gatcaactct cactcaacag
    21421 ttgattaagt tgacttactt ttcaacttcg acttccgacc agactatttc tcgtaaggct
    21481 caggaagctt ggttagcgat tcagttagaa caaaaagcaa ccaagcaaga aatcttgacc
    21541 tactatataa ataaggtcta catgtctaat gggaactatg gaatgcagac agcagctcaa
    21601 aactactatg gtaaagacct caataattta agtttacctc agttagcctt gctggctgga
    21661 atgcctcagg caccaaacca atatgacccc tattcacatc cagaagcagc ccaagaccgc
    21721 cgaaacttgg tcttatctga aatgaaaaat caaggctaca tctctgctga acagtatgag
    21781 aaagcagtca atacaccaat tactgatgga ctacaaagtc tcaaatcagc aagtaattac
    21841 cctgcttaca tggataatta cctcaaggaa gtcatcaatc aagttgaaga agaaacaggc
    21901 tataacctgc tcacaactgg gatggatgtc tacacaaatg tagaccaaga agctcaaaaa
    21961 catctgtggg atatttacaa tacagacgaa tacgttgcct atccagacga tgaattgcaa
    22021 gtcgcttcta ccattgttga tgtttctaac ggtaaagtca ttgcccagct aggagcacgc
    22081 catcagtcaa gtaatgtttc cttcggaatt aaccaagcag tagaaacaaa ccgcgactgg
    22141 ggatcaacta tgaaaccgat cacagactat gctcctgcct tggagtacgg tatctacgat
    22201 tcaactgcta ctatcgttca cgatgagccc tataactacc ctgggacaaa tacccctgtt
    22261 tataactggg ataggggcta ctttggcaac atcaccttgc aatacgccct gcaacaatcg
    22321 cgaaacgtcc cagccgtgga aactctaaac aaggtcggac tcaaccgcgc caagactttc
    22381 ctaaatggtc tcggaatcga ctacccaagt attcactact caaatgccat ttcaagtaac
    22441 acaaccgaat cagacaaaaa atatggagca agtagtgaaa agatggctgc tgcttacgct
    22501 gcctttgcaa atggtggaac ttactataaa ccaatgtata tccataaagt cgtctttagt
    22561 gatgggagtg aaaaagagtt ctctaatgtc ggaactcgtg ccatgaagga aacgacagcc
    22621 tatatgatga ccgacatgat gaaaacagtc ttgacttatg gaactggacg aaatgcctat
    22681 cttgcttggc tccctcaggc tggtaaaaca ggaacctcta actatacaga cgaggaaatt
    22741 gaaaaccaca tcaagacctc tcaatttgta gcacctgatg aactatttgc tggctatacg
    22801 cgtaaatatt caatggctgt atggacaggc tattctaacc gtctgacacc acttgtaggc
    22861 aatggcctta cggtcgctgc caaagtttac cgctctatga tgacctacct gtctgaagga
    22921 agcaatccag aggattggaa tataccagag gggctctaca gaaatggaga attcgtattt
    22981 aaaaatggtg ctcgttctac gtggagctca cctgctccac aacaaccccc atcaactgaa
    23041 agttcaagct catcatcaga tagttcaact tcacagtcta gctcaaccac tccaagcaca
    23101 aataatagta cgactaccaa tcctaacaat aatacgcaac aatcaaatac aacccctgat
    23161 caacaaaatc agaatcctca accagcacaa ccataacatt tatcacccag atttttctgg
    23221 gtgattttgt catagcacta ttgtatttat gtgcttaaca aaagcctgag aagatgtctt
    23281 ctcaggcttt ttctgtacta taaacctcat tggaaaagat agagactgct tccgtaatct
    23341 tgttaagagg ttttactaaa tactagtctt tcttcgtttt tactacaaag agagcagata
    23401 gggctagact aacacttgct aggaagaggg ctgtgtcaga ttgactctca cctgttgctg
    23461 gcaagatttg ttcaggtgtt tctggtctag ctatttcttg cttataatct ggcgtcttac
    23521 cttgatcgga aactaaagag ctaagtttaa actcaggttt ctctacagtt ggagcatctg
    23581 gtttcgtagc agaatctttt aattggttga gataatctgc aaagtattcc accattcttt
    23641 ccgtcaagag atggttatcc gtcgcatatt tcatgcttgc ctgaacgcta gctacttcct
    23701 cttgattgtc tttgtctgtt aatttctgag ctttagccaa agctgcttca tagcctgaca
    23761 agtcaagagg tgtttctgct acctgtggac gagtaaagat gagttccgct gcagattggt
    23821 atttatcgcc accatctccg taagtcttag tacctgtaag gacaattttc ttagccttaa
    23881 ttgtcttacc aaaatcaatg tcttttggct tattgttatc tggccaatca gttgcagtaa
    23941 aggtatgctc cttgccagac tcatctgtca caacaagttt cacatcacgc aagttaccat
    24001 ttgaacctga tccacgtgga acgtaacgaa gtccagtgat ttcagttggt tctttcaaga
    24061 ccatggttgc aggcttgcct acatctccac cattccaaga tgtatgccat aaactagata
    24121 agtttccatc aaaggcattt gcaagacctt cttgagcctg agcaggagct gtaagacttg
    24181 caaagtcatc tgctaccaaa gccgttttct tctgaaccaa agcattcttc aaggcttcaa
    24241 tcttggcaat ctctgcacgc gcttcttcca cactgatatc atcatcggcc tgactaaggt
    24301 taaagaccgc ctctttcaaa gcatccatag actctttggt gtagttagtc atggcaaccg
    24361 ttggcaagta gttcttcaga gcattttctg tcaacatctt acctgttagg gtaatttctt
    24421 cgatttgaag attatccatc atgaagtcgt tataaccacg gaagttggca tttccaccag
    24481 aatcaccacg agtattactt gcattgccag ttgagtagat acctacccaa gtatcccctg
    24541 tttctgcacc tgtcacaagg aaggttgcct tcttggcttt cttagaatct gtccaagtat
    24601 ttggcaattc atgcatttcc aagttgcttg cttgagtgcc acgacgacct gactggaatt
    24661 ctcccttacc gactacaaaa gcataggtat tgtctgaacc tgcttcgtat tcaaaggtta
    24721 cacggtaggt cttacctgct tcaaaacgga agttttgtgg gatggtttgg taaaccaagt
    24781 tacgacggct cactagtcca tttgtcttga gtgaccaatt tccttcgata acatcatcga
    24841 ctttcttacc gttccaacca cgttgtgtat atggatcgtg tttttcagac aagtgagtgc
    24901 ggttgtcttc gacaccttcg acaccaccca ctacaaatgg gaagattcct tgagctacat
    24961 tttcaaagtc ttgcttgaag gttccttgac ctgtatcgtg cttgtctcca tacatgcttg
    25021 aattgttttc aaaggtacga atttcatcaa agtaagttgc ttgatcacca gcttcacgac
    25081 tcaatgtcag agtaacattt gagacgtccg atcctgttgt aaagaaggcg tacatgtttt
    25141 ggaagtaact tgtatcgtta actgtagcat tgttacgacg tgtattgtgg gcgtaggctt
    25201 ttacatagtt gagggcgaga gacttattgg tataagtagt cacttctttt tcaccagtat
    25261 tcacagtgat acttgccttg gcattactac ggttatcgac accgacataa acggcatact
    25321 tggtatttgg tttcaagcca gttaatttct gagtgagact aactttttct ttgtttcctt
    25381 gaatacgaag catatcgttt gccccttgag acttgacaat ttctgcctta gaagcatcgc
    25441 ctgaaatggt ccaatgtttc aaggtaccac tgttaaatcc ttggtcatag atgtgcatgc
    25501 cttcactcca tgacatttca ggattggttt gttttgaacg atagagaacg tatggttgat
    25561 ttgctagaag atctagggta attttaccat cttttacagt tagttcttgc tcttctgtct
    25621 taccttggtc agttagcttg taaaggtaaa ccttgctctt tgcccaatcg cttggaaggg
    25681 tccaagttgt tgcaccggcc tgcgtattga agtagtacat cttttcctta tcagtagaaa
    25741 gtttcttacc atttgcatcc cagttccaag gagtcaagta agctgaacca tcttggatga
    25801 cacgtccgtt gagagttact gtacgttcgc gatattgtgg actattgaca tcatttgact
    25861 tacgagttac aactacttta ttattgtcag catctaccaa ttccactcgc atttctggag
    25921 tccatttata ggtactaccg ttatcggtca tagtcaccgg tgtaccattt tcccatttac
    25981 ttacagtgaa gtgttggaag tacttagtca tgacgtcatg ggcaaataag ttggttacat
    26041 agccattgta gtcacttctt ccctgccagc cttcaaagtc tttcatgctg tagccaccta
    26101 gcagtggata gttggctgca ccaccataac ttctgtagtc cccaacccaa gcatcttttt
    26161 ggtggttacg gataaagcgg gtgatggcac tgttgatacc tttattggtg tagccaccgt
    26221 aggtcaagtc agctgcccag tgatggaagg tagagtcgta ctcaccacca tggccccact
    26281 cgatcgcaaa gcgccagcct tgtttgttaa tttctttagc aagaacgtgg gtagcccagg
    26341 caccgttatc acctgattga ccattacccc aaacgtccac atagataaag tcgagaccgt
    26401 caccaagttt tttcttcaaa tcttcccaac gtgccaaacg accatgagct aggtcatagg
    26461 cagcatcaat gttgatacct tgatctagcc agttccaacc atagctatag cttccatctg
    26521 gattcttacg gagaattttt tcattgaagt atttagactc aggataagtt tctgaagcgt
    26581 taacgtggat acctagatga gctccatatt tcttagcctt ctcaattagg gtcttgaagt
    26641 cttcgacacc accgatacgc ttaccaatat cagcatagtt caagtgacca gagtcatggc
    26701 cttcgctacc atatccttta aggagaacac cttgcccaag accatctgtg tggagattga
    26761 ttttcttgat accatccaag gtcataagga atggattttg tgcttgagaa ccaaagttca
    26821 tcgcgatacg gtaagctgtg atatccttaa ctttttccca accttgagga ttgttcataa
    26881 tgctacgata agcaatggca ccatcttgcc aatcgacttt cttgtctgca ttggcatctt
    26941 cagtgataac aaccttagca cttggaagtt ccttcgtgta ttctgggaaa acaatgccct
    27001 tataagcttt ttcccattgc cattcagagc tgtggattcc tacatagttg gcatttccga
    27061 ctgtttcttt ataagctgtc aaacgagtcc agtcattcga accaccacca tagctatttt
    27121 gagagttact ccaaacacca gcagcaagct tatctgtaga aacaaatcca tacatgtaac
    27181 ccttggctag gtctttcatt ggattggtta catcgatatg atcatctccg ctgacatgcg
    27241 tattgtttga catggttgcc ccatcaaact tagcaccagt ttgatcacta gaaacagaga
    27301 ctaaagcatt gccgaggaaa ctaatagaag aaagtagttt tctttcgtca tcaatctttt
    27361 gacctggagt gacttgattg tggttgacaa tcttggtcac atcaaagtgc aattgattgt
    27421 ccacaacttg caagcgtact gtcatttccg cattgattaa gtgagcatca tcgcgaagct
    27481 tcatcaagta ctctgctgtt gtctcattga ttttcttata agtgacttca ggggtgattc
    27541 ggtggttatt gataaagact tggttgaact gttgcacctg tcctggcaaa gtatgtccat
    27601 tcaagctgta ttccttgaca cgagggaagg cttggtcaat cactgctttg aggaccttag
    27661 actggatcgt atcataagtc accttgctat cgtcaacaac cggacctgtt tctttctggg
    27721 caggagtatc atccgctttt acgccttctt gattgtctgt tttaacgctg acaactgtac
    27781 ggtcattgcc ataagttcct gccttgagaa ggatcttctt ctcattttta agattatcat
    27841 tgaccgcccc aggcagtgtc actgtgtcaa agagattgac atcgttattg ctggcattga
    27901 gttgtccatc tgacttgaga gtgatagaaa gacggtttac agaacccggt tctggtgcaa
    27961 ctacacggtt gcctttatac catgtgctat tacctggagt tttgtattcc cagaaccagc
    28021 catccttgtc ataaccgaca aaaacattat tattggtatc tttaaatttc aggaagacac
    28081 caaagcgtga tttgcccttt tcagaatctt ctttgaaggt taaatcaaca gttgcatttc
    28141 cattggcatc aacggtcaag cccttctttt caaacagggc tggtttgcct gcgttatcat
    28201 tttgagcagt tgagaatagt tggttgtagc ggacaccttt ttcttctcgg atagtgactg
    28261 ttccctgttg ttcttttttc tctaccgttt gccattcagg agttaccgtc ttaggtgttt
    28321 caggtttagc agctgcaggt ttatcctctt ttggtttttc ttcagccggt ttttcttttt
    28381 caagtgctaa tagttcttct tctgtcttag gtccatctgt aacttctgga gaaccttggt
    28441 cttctcccac cttaggcgct tcaaaatcac gcccatcatt ctcgtttgct gttgcaagag
    28501 cagtagctaa atcagctggt aagttcgccg tggaaccaga ctgcacgcta tctgcaagaa
    28561 ccggacttgt cccaaagaat gcagctccaa tcataacaga agcaacacct aatgaaaatt
    28621 tccgaatact atatttacaa cgtttttcaa ataatccttt attcataaac ttctaaatcc
    28681 tttcattttt tagcaagcgt ttacatttcc tttttaaaaa atactccttc ctctgaaagt
    28741 gcaatgctta accctgataa ataaaaaata attgtacttt taatataaca aataatagct
    28801 gagaaagcaa taggaaacaa gtaaaattaa caaaaatttt ctctattctg acaaaaagag
    28861 gctccaactt ttgggggtca gtacatttag aatctttctt tacaaatgta aaaaaggatt
    28921 cagcattcaa gagaaatact aaatcctttc ttatattttg caacagttat ttcacatgtt
    28981 ttgcgagatc ttcttgagcc tttttattag actcttcttt ttctttcatc catttttcct
    29041 gagctttttg gtattcatct acagtgactg ccttgtcttg aagttccaag tatttataca
    29101 agactggttc acttgtacct ttatttcctg acaatgcaaa tggtattgta aatggtacca
    29161 tcttagacaa gattgggcgc cctgtacgag atgtagttgg aataatcaaa gcactatctg
    29221 tcaaccaagc ttgggctgca gcgtatttat catagcgttt agcaacatct gtagcctcat
    29281 caccagcctc agtgaccaat ttttcgtagt catatagacc tactttttta gcagctacat
    29341 tatcttcccc tgagtcaaac cctaaatatg ttttagtact ttctcctaca gaaggtttga
    29401 taatatcaag gtaggttgat ggatcggcaa agtctggacc ccaaccgaca ttatctgata
    29461 aatcccagtc ttcgccagca gcattttcag caaaatatgt aatattgttt acttcgtctt
    29521 tttgtagttg ttggatatca ataatgacat tatcagctcc taaagttgct tccaaggatt
    29581 gtttcataga ttggacgcgc tgaacttttg tagttgctgt ctggtcaact ggcatatcca
    29641 aatgaattgg gaaagtcaca ccttctgctt gtaaggctga tttagcttta gcaaattcag
    29701 ccttggcttt ttctggattg taaagaccat cctgagaatc tgcaagatta acatccttcc
    29761 attcatcccc ataagtgacc aatttctctt tgaccatatc gccaaagttt ttaccatctg
    29821 cttgaacaaa tgttggtgga acaaagatat tacgtaagat tttgcttgct ccagtttgtc
    29881 cattcaactg agaggcatag gctgtacgat caaaaccaaa ggcaatagcc tgacggaaat
    29941 ccttgtttaa gagagccttt ttagtcgatg ccttttgttc gtcgctggtc ttagatgtat
    30001 atttatagga ctgacggtca atatttgtac caactagata cgtaatagag ccttgttgag
    30061 tatagacaat attgtccttc atactcttct caagctctgc gaaacttgca cttgttggat
    30121 agagacgagc tgctgtaagg ctaccatctt taaagttttc tgcaggtttg ctggtatctt
    30181 gaccatccca gaatgacaat ttaactttgt caacatgcac attgtcctta tcccagtagt
    30241 tcggattttt cgcaaattca acagaggatt ttgtcacaat ggatttcaac aaataaggac
    30301 cgttatacaa gagactactt ggatccgtcg ctttggcaaa atcatcccct tttgagttca
    30361 aaaactcttc attaactggc gcaagcacac ccatggttgt cttagaattc cagaagcttt
    30421 ctggtttgtt caaagtgtac tgaactgtct gatcatccaa agccttaatt cctacttgtg
    30481 agaaatcttt gatttcccct tttacatagg catccaaccc tttgattgat tcttgaacaa
    30541 ggtaaagagc atctgatttt ttatcagcag catattttag tcctgttaca aagtcttgag
    30601 ctttgactgc cgcgtattct tcaccttcag aagtatacca ttttgcatcc ttacggatag
    30661 tataagtgta agtcaatcca tccttggata cagaccaatc ctcagccata gacggcacaa
    30721 agttcccgta gcgatcattt tctagcaaac catcaaccac gttactggta atatttgctg
    30781 tcgcagcctt agcagttgtc aaatagttga ggttatcagg gtctgtctca taaatgtatg
    30841 agaatgtctt ctcaccttta gtgcttgaac ctgatccaga gcatgcagct aaagtagtcg
    30901 ccgccaataa tgtcacgccc gcaagggcaa gtagttttga acttttcatc cttttctcct
    30961 ttaaaacttt ctctccatta tagactcttt tcagaaagtt gtcaacagaa ttttcagaat
    31021 ttttgaaaat tatttttcaa ataacatctt tgcaaaaaat atgaatatcg taaccgccca
    31081 ataacgaagt ctattgaaaa atctccagac tagagaactc acggatagtt cctaatctgg
    31141 agatttctta tttgcacttt tcttgtacaa ctttagtcca cggtaaatag gcctctaaaa
    31201 tctctttgtt tacgagagtt tcctcgtttg gaagacattc tagaagatag gatagatatt
    31261 tctcgctatt taat
//
LOCUS       DAVBCR010000021        31017 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCR010000021 DAVBCR010000000
VERSION     DAVBCR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.021"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(297..515)
                     /locus_tag="VQ508_000845"
     CDS             complement(297..515)
                     /locus_tag="VQ508_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489870.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(915..1115)
                     /locus_tag="VQ508_000846"
                     /pseudo
     CDS             complement(915..1115)
                     /locus_tag="VQ508_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542046.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1269..2900)
                     /gene="rlmD"
                     /locus_tag="VQ508_000847"
     CDS             complement(1269..2900)
                     /gene="rlmD"
                     /locus_tag="VQ508_000847"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2489871.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2974..3948)
                     /locus_tag="VQ508_000848"
     CDS             complement(2974..3948)
                     /locus_tag="VQ508_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2489872.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4022..4636)
                     /gene="pmp23"
                     /locus_tag="VQ508_000849"
     CDS             complement(4022..4636)
                     /gene="pmp23"
                     /locus_tag="VQ508_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2489873.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4636..5610)
                     /locus_tag="VQ508_000850"
     CDS             complement(4636..5610)
                     /locus_tag="VQ508_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2489874.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5618..6874)
                     /locus_tag="VQ508_000851"
     CDS             complement(5618..6874)
                     /locus_tag="VQ508_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2489875.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6936..7367)
                     /locus_tag="VQ508_000852"
     CDS             complement(6936..7367)
                     /locus_tag="VQ508_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489876.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7369..7971)
                     /locus_tag="VQ508_000853"
     CDS             complement(7369..7971)
                     /locus_tag="VQ508_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2489877.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7955..8794)
                     /gene="prmC"
                     /locus_tag="VQ508_000854"
     CDS             complement(7955..8794)
                     /gene="prmC"
                     /locus_tag="VQ508_000854"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2489878.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8794..9873)
                     /gene="prfA"
                     /locus_tag="VQ508_000855"
     CDS             complement(8794..9873)
                     /gene="prfA"
                     /locus_tag="VQ508_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2489879.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9870..10460)
                     /locus_tag="VQ508_000856"
     CDS             complement(9870..10460)
                     /locus_tag="VQ508_000856"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2489880.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10583..10765
                     /locus_tag="VQ508_000857"
     CDS             10583..10765
                     /locus_tag="VQ508_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2489881.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10902..12275
                     /gene="mnmE"
                     /locus_tag="VQ508_000858"
     CDS             10902..12275
                     /gene="mnmE"
                     /locus_tag="VQ508_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2489882.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12322..13257)
                     /gene="dapA"
                     /locus_tag="VQ508_000859"
     CDS             complement(12322..13257)
                     /gene="dapA"
                     /locus_tag="VQ508_000859"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2489883.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13313..14389)
                     /locus_tag="VQ508_000860"
     CDS             complement(13313..14389)
                     /locus_tag="VQ508_000860"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2489884.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14731..14803)
                     /locus_tag="VQ508_000861"
     tRNA            complement(14731..14803)
                     /locus_tag="VQ508_000861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14768..14770),aa:Thr,seq:ggt)
     gene            complement(14903..15412)
                     /locus_tag="VQ508_000862"
     CDS             complement(14903..15412)
                     /locus_tag="VQ508_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2489885.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15410..15506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15535..15966
                     /locus_tag="VQ508_000863"
     CDS             15535..15966
                     /locus_tag="VQ508_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2489886.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16048..16425
                     /gene="mscL"
                     /locus_tag="VQ508_000864"
     CDS             16048..16425
                     /gene="mscL"
                     /locus_tag="VQ508_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2489887.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16467..17561)
                     /gene="hemH"
                     /locus_tag="VQ508_000865"
     CDS             complement(16467..17561)
                     /gene="hemH"
                     /locus_tag="VQ508_000865"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2489888.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17639..18471)
                     /gene="srtG2"
                     /locus_tag="VQ508_000866"
                     /pseudo
     CDS             complement(17639..18471)
                     /gene="srtG2"
                     /locus_tag="VQ508_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18549..19547)
                     /gene="srtG1"
                     /locus_tag="VQ508_000867"
     CDS             complement(18549..19547)
                     /gene="srtG1"
                     /locus_tag="VQ508_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2489889.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19565..20761)
                     /gene="pitB"
                     /locus_tag="VQ508_000868"
     CDS             complement(19565..20761)
                     /gene="pitB"
                     /locus_tag="VQ508_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2489890.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20806..21357)
                     /gene="sipA"
                     /locus_tag="VQ508_000869"
     CDS             complement(20806..21357)
                     /gene="sipA"
                     /locus_tag="VQ508_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2489891.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21357..23309)
                     /locus_tag="VQ508_000870"
     CDS             complement(21357..23309)
                     /locus_tag="VQ508_000870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2489892.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23439..23942)
                     /locus_tag="VQ508_000871"
     CDS             complement(23439..23942)
                     /locus_tag="VQ508_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2489893.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24301..25524
                     /gene="pepT"
                     /locus_tag="VQ508_000872"
     CDS             24301..25524
                     /gene="pepT"
                     /locus_tag="VQ508_000872"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2489894.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25560..26189)
                     /locus_tag="VQ508_000873"
     CDS             complement(25560..26189)
                     /locus_tag="VQ508_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489895.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26154..26375
                     /locus_tag="VQ508_000874"
     CDS             26154..26375
                     /locus_tag="VQ508_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489896.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26646..26994)
                     /locus_tag="VQ508_000875"
                     /pseudo
     CDS             complement(26646..26994)
                     /locus_tag="VQ508_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27099..27659)
                     /locus_tag="VQ508_000876"
                     /pseudo
     CDS             complement(27099..27659)
                     /locus_tag="VQ508_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27885..31004)
                     /gene="phtE"
                     /locus_tag="VQ508_000877"
     CDS             complement(27885..31004)
                     /gene="phtE"
                     /locus_tag="VQ508_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2489897.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8836 a         6364 c         5687 g        10130 t
ORIGIN      
        1 aaaggatcta gtttttattt ttagttaaat tatgtctata tttttcatta actcatcaat
       61 aaaaatttgt ttgctaatat ctctttcttc tgattttgtt tcaaggatta ttgctacatc
      121 attcatattt ttaggtaaat accaaccatc tggcttatct gaatatccct tgaagcctaa
      181 ttgattaaaa gtagttattt gaccagtacc ttgattgatg ttttcttcaa cttcattaaa
      241 gcctatactt ctcttgcata atccctaact tgatcttctg tccttaaagt cattttctat
      301 tcctcgtcac taaaattatc ttcaaaacta ataatatcat taggagtgca agataaagcc
      361 ttacatattc tttctaaatt cttaaatgta attggtttat cctttcccat ttgtgccata
      421 acatttgttg taagtcctgc catagctatt acatctgttt tctttagtcc tttttttgct
      481 aactgtatcc ataatggttt atagttaagt accataatat ccctcttttt ttcttcatta
      541 aatttattta attatagcat aaatattgat ttcattcaat cttacattga tttagtgatt
      601 tgttttagag aatttgttgt gttattccgt ccttattttg ccataaattg ttttaagtgg
      661 gacctcttat ccttaccatt tttacctcct aattttgagt ataaaaaaga cgatagagtt
      721 tttaatttct atcgtctgag atttacatat taaattttca tcacaaaata agactcttta
      781 aagaatcttt aaattcattg gtatgctgct tacaatatcc attattaaaa tggatactat
      841 aagcagcaca ccaactattt attttttgag ttttaaactt tggtagaagt cttgaactcc
      901 ttatatttac ataactattt gcctctataa ctatttgcct ctataactat tatgacacga
      961 aaccaacaag caagttcaag catctccata tctttattca aatgaggact aagatattca
     1021 caaactttat cataaacctc tttatctttt ttcataaaag gggcatacaa cttagcaaat
     1081 ttatcctaaa attttttgtt gcctttatca atcatttcaa cgctccttta aatataaaaa
     1141 taatcctatc aatctttata ttgagcctat tttctaattg ataccttgca agaaaggaat
     1201 aatagctgtt cctataccaa gtaggaatgt aataaatgca gaaagtcata tactgtcatt
     1261 cttttctatt aaatcatttt gaagtgtctc aaagcatcca tgatggcttc ttctttttca
     1321 ggtgtacact gtggaataat ttgtttatcc tttttagact tgttgtaatg ttctcgtaat
     1381 tctattccac atttcttttt tatctgtgca atatataatg tcgaaacttt taattcaaat
     1441 ttattccaaa catattcttt gatttgagca tatgttgctt tgctctccgc acttgtcaaa
     1501 tccatctcat ccagctcaat ttcaacactt atgtgcttat cgacatcgag tttggacaaa
     1561 agtgctaccg tctcgacgtg atgcgtttga ggaaatagat ccaccggctg gactttcttc
     1621 aattcatatc ccaattcttg gtagagtttg atatcacgcg ccatggttgc gacattgcag
     1681 gagatatagg cgatgcgatc ggctcctgtt tgggcgcttg ctttgataaa gctttctgtc
     1741 aagcccttgc gtggaggatc aaccaagata acggttggtt gaataccttc cttgagccat
     1801 ttcttcatgg cattttcagc cgtgtcacag acatagtggg cattagtaat cttgttcaaa
     1861 gaagcattct tctggctatt ctcaaccgct tctggaatca gttcaacacc gtagacttct
     1921 ttgacatgct tggcgactga taaaccaatg gttccaatac cagaataagc atcaataacc
     1981 acgtcatctt tttttaactc tgcaaagtca atggctgttt gatagagttt ctccgccatt
     2041 tcagtattga cttggtaaaa ggctgggcca gcgatttgga agtcatttcc caacatctgg
     2101 tccgtaatat agtcttgacc ataaagagtg caccactcct taccaaaaat cgcattggta
     2161 ttctggtcgt tgatattttg catgacagac acaatctctg ggaactgctt gataacttgt
     2221 tcaatcaatt ggtcaacacg aaaaactttt ggacgagttg tcaccaaaac gaccatgatt
     2281 tgtcctgaat agtgaccacg acgcaccaca agattccgaa tcaatccaga ctgttccttt
     2341 tcgtcataag gttttaaatc aaaacgacgg agcaggtctc gaagagctac tacgacttgg
     2401 tcaatgacag gatcctggat aaagaaatct tcaaggggca tgaggttgtg cgaattctta
     2461 cggaaaaatc ctgtttccaa gacaccattc actcgacgaa cgggcacctg cgccttattg
     2521 cgatacttga ctggatgttc cataccaagc gtttcagcaa cttctacatc tgcaattcca
     2581 gcaatcttgt agagactgtc cttgacttgc ttggttttaa acttgagctg ttctggataa
     2641 gaaaggtgtc ctaaatccgc gattcctgaa cgcaggtaag ccaaatctag atcttgatta
     2701 cggtgtagtg actggacaag gtatttttca acttttccaa agccaatctt tttattgacc
     2761 ttgaggacac gcatgagaat tttttcactc ggtaaagcat tctctacaaa aaagaccaaa
     2821 ccatctacct tggcaactcc tgccccttca tgggtcaaat caacaatttc aacttctaca
     2881 atatcatttt tctttaacat tcttttctct ttctattggc cgacaaaaaa gacggcaacg
     2941 ttacgattac catccattat accatgaaat ttcttaagaa ccaaaactct tgaagaagtt
     3001 gacaagggct tgccagaggg agcttaagaa gttaccaccg tcctctagcg ccttatccgc
     3061 atcaaagtta aggttgatat ttttaaaact gtcgccagct tgtgatacga tgctttgttt
     3121 aaggtcattt agggttttag tgaaatctgc attgctgagg atatcactct ttgagagatt
     3181 caaggcaaaa ttgatgatga tattgatctg gtttcctgtt atgacctgat caagtttgta
     3241 attttttaag gtatcttcaa caatcttgcg gacatcttct tctgtcagat ttcccttgct
     3301 ttctttagct ttggcgagtc ctgacttgat atcagctagg gcaacgttta atttattagc
     3361 atcatagcct gatttgtcct tgttttcagc attgatatct gacaaagcct ttagctcttc
     3421 ttgagccaaa tctttattgg cttgcggcac cttggctcca ttagcttcta gcgaatagta
     3481 gatcccagcc aaagcactct cacctgtaac tggaataggg gctgctacag tgattttggc
     3541 atgttccata cccagcgtta ctgctgcgtt tcggtacata tcctgagtca ccttagtgat
     3601 attttctggt gtttcaatct tgacctcaag tggcgatttg tcacctagct tttgaatctt
     3661 ggctgatgaa tacaactgta agctagagtc attggccaca ttcatgattt tagaataaac
     3721 atcaggtgtc atggtcttga gttctttggt atctgttgag gcattgtagc ccagtttttt
     3781 aagagtttga tttttttggt cttcagatag ggaggaacct aggacatatt caggttggac
     3841 ataggtttca tcgataactt tttgaacatc tgttgctgca tggacgctat tcatagctgt
     3901 tactgcccac aagaccgcag cactggtcag aaagagtttc tttctcatag ggaatttcct
     3961 cctttacttt tctagagtaa tatatctatc ttaaagaaaa cttataacaa aaacacctgg
     4021 tctagccaga tgttgaaaag agagtgaaac atttgatgat gtaaaggtta agtcgtacct
     4081 gtctagaata ataatagttt cctccattta catagagttc agcaccgtga aaaatggaaa
     4141 tggggtgaat ataactataa gtctttccag tcgtattacc aagcaagggg gcaacagtct
     4201 cacgagagta ctgtttggct agagccaagg tattttcctt gccattttgg gcgataaaat
     4261 cgatataggc aggtccaaaa ttataggctt gaacagctgt ccagatatct acccccttct
     4321 tctgcgccag atagagattg cctgtcagag tttgaatgcc ttgccgaatg ctagaggcat
     4381 tatcattgat ggtgttggtt gaaccacttg cagactcact agactgcata acatcgcctt
     4441 cttttccttt tgtttcagta taaatcatag caagcacaag ctcttcgttt gctggggtgt
     4501 ctttttcact caagatttct cgcaccatgg gttgataggt catgacttgt ttgacatctt
     4561 gatgaacgcg gtaagcttta tagccagcaa aaaggaagac tgctagtaca agcactcttc
     4621 gaattcgttt aaacattatt tactttggat atcctcgata tttttgatta agatagagta
     4681 ggttccattt tccttttgga taaactcaac agactcggcg tcttgataga cgttattggg
     4741 aacgatgagc tcaattccat ttgataagga gagtttttgg ttttcaaatt tctttaattg
     4801 gcgactggca tcaatttcat caaattgaac aggttctggt acggcttctt tgacttggtc
     4861 aataaagctc aaacgagccg tcagattgtt gtcaaaaagg tcattagcca atttctcagg
     4921 tgacaattca ttgctttctt ctaggttgtt gaaaatcgct gatttgacct tggattgaaa
     4981 ttgaaaatca tctgtgttaa aagattcagc aattctctgg gctgtttttt ccagttcctt
     5041 gatagatttt ttaggagaaa tcttaggagc gacagcaaga agattatctg aaaaatagtt
     5101 caaaaaagtc ccgttgtact tgattcgttt ttcaatcagg tgatacttgc gactctgaag
     5161 attgaccacc aaggcctcgt cagcacccgt tccaaatcca ggcaggttat tctgagtcag
     5221 cttgattgga ttatcaactt ctcctccgag gtgggtcaag gtctcccgca gggcaattcg
     5281 caagaaagcg aaatgttcta caccttcttt agaaaattga acaaaaatca agtcattggt
     5341 cttgagattt tcagaaatgc taaactcctc tttccagaga ttagccagcg ttactgatgt
     5401 ctccaacaaa tcgtctgtaa tatgattgaa gaagggattt tcttcttcga aaatcccagt
     5461 cttggcttca tctgaataca catgttcaat ttttttacgt aggtattctt cgatttttgg
     5521 agtaatattg agaaacttat ctgctaagaa cagctcggta tcatccggac tgaactggtg
     5581 aataatggct ttcttaatat aaatgtccat aaaagtttta ttcctcgtat aatgggaagg
     5641 catctgtcaa ttctttgact gcacttctca cttcttctaa tacagcctca ttttctgaat
     5701 tcttaagggt tttaatgatg agttcagcca ctttgcgact ttcttcttca ccaaatccac
     5761 gtgcagtaat agctgctgct ccgatacgaa tcccacttgt cttgaatggt gacaaggttt
     5821 cgtaagggat tgaattttta tttagggtaa tattgacttc atccagcaag ttttgagcaa
     5881 cttttccgtt ttctacaacc ttagtcacat caacaaggaa gagatggttt tcagttccac
     5941 ctgaaataat acggaaatca gggtcttgca agaagacatc tgccatagcc ttgctgttct
     6001 taattacatt ggcagcatat tccttgaagg ctggatccaa aacttctttg aaggaaactg
     6061 ccttagccgc cacaacatgc tctaaaggac cgccctgaat acctgggaaa atagctgaat
     6121 tgattttttt agcaagttct tcgtcattgg tcaaaatcaa accaccacga ggtccacgaa
     6181 gggttttgtg ggtcgttgtt gtagtgatat gagcgtatgg cactgggctt gggtgaagac
     6241 cagctgcaac caagccagcg atgtgggcca tatcaaccat aagcttcgca ccgacagcat
     6301 ctgcgatttc acggaatttt gaaaagtcaa taatttgaga ataggctgaa gcaccagcta
     6361 caatcagttt tggttttact tcttgggctt gtttcaagat agcatcaaag tctaagagtt
     6421 ccgtttcagg atcaacacta taagaaacaa agttgtaggt ttgaccagag aagctaacag
     6481 gagccccatg agtcaaatga ccacctgatg ccaaatccat tcccataacc gtatcacctg
     6541 gctcaatcaa ggacatgtaa gccgcacagt tggcctgact tcctgagtga ggttggacat
     6601 tggcaaattt agcaccgaaa atttcttttg cgcgttcaat agcaagagtc tctacaacgt
     6661 ctactacatc agttccacca taataacggc gtcctgggta accctcggca tatttatttg
     6721 tcaagataga cccttgagct gccataacag ccttggaaac tacgttttcc gaagcaatta
     6781 actcgatatt attttgttgg cgttcttctt ctttggcaat agcattccag agatcagcat
     6841 catatgcttt aaaatcatct ttgtcaaaaa tcataggtct tctcctttat tgtgtgacta
     6901 gtccattagt ttgattttac aataagaaaa tcaaactaaa agatgcgaat aaaccgtttc
     6961 tgcattttat cacaagtata gccaactttt tcataaaatg catgagcacc cagacgatga
     7021 ttggcagaat ttaagcggat aaacccataa ccacatcttt tggcttcttg ttccaaccct
     7081 tgtagtaaac ttttaccgat accttgacct tgcgcttgag gtgaaactgc taaagctaag
     7141 atattaaatc ctgctttgga atagagtgat tcgtaaactt cagcgtggac atatccaagt
     7201 aagacatgat tagctgcatc ctcatagcca agtaggaaat gatgggaatc ctgagacagt
     7261 ctagctagtt ggctagccgt ttcctctgga ctaaaagtat aacccaaagc ctcttggttg
     7321 atgtcacata tcgctttcac atctgtttct tgcaaatctc ttagcatttc aagcctcctc
     7381 aaaagaaatc tctggcaacc gagcaagaat atcttctcgt ttaattgcgc cttggcgtaa
     7441 gattttcacc ttgtctccag acaaatccac aatagttgaa tcctgtccag ttagaaaagc
     7501 atcgtcttcc agacccagaa cctcttggtc aaaatccttc agaatttgtt caaaggttac
     7561 accacttgcc tgacctgaga tattggcaga cggcccaatc aagggacctg tctctcgaat
     7621 taaatccagt gtgatagggt gactgggcat ccgaaatcca atagttgcaa ggtcagaatt
     7681 tacccaatag ggaactcggt cattggcttc gagaataatg gtcaagggac ctggcaaaaa
     7741 ggtctctaca agtttttgta gataagttgg ctgattctta gaaaagtgca agatatcctc
     7801 gaaagaggcg atattgagat tgagtgcctt atctctagga cgacgtttga gttggtaaac
     7861 atggtcaact gctttttcat ctaaggcctt ggcaaaaaga ccataaacag tctctgtagg
     7921 tagaacgaca gctccaccat tttccaactc ttgtctaatc ctgtccatca tcaaccacaa
     7981 ccatcctatt ttgaccaaat tggtctttga gtgttcgaac ttttttttca ggaagatgtt
     8041 ttctaaaaag ttcgggaaca ctttgacctt gcttgtatcc aatttcaagg taaatcttac
     8101 caccatcctt gagatagtct tttgcatctt ccgcaattct acagtaaata gctaggccat
     8161 cctcgtctgc aaagagagct agatgaggct ccgaatgcaa aacattcaag cctacctctg
     8221 actcatcttc acgagagata tagggtggat tggatacaat tatatcatat ttttcagaaa
     8281 tttctgcaaa acaatcagat tttttaaaaa atatattaag attttgatcg ctagcattct
     8341 ctgatgcaag ctctaaggcc tcttgagaaa catctgctgc tgtcactgac caatctggtc
     8401 tattttttgc tagagcgaga gcaatagctc cactacctgt tccaatatcc agaactgaga
     8461 gattcgtctc aagattttca gttagaataa gctccactaa ctcctctgtt tctggacgag
     8521 gaatcaaaac ccgttcatcc acctttaaat gcattccata aaaatctgcc tgtccaatga
     8581 tgtactgagc tggcttgtga gctgctagtt gctggtaaat atcttctaca aattgttttt
     8641 cttcctctgt tgtcacctcc tgctggaggg caaaaataaa gtctgtaaaa gatagatttt
     8701 tcagactacg atagacaaaa gagaggcttt ccgcttcctc tccttgtctt atcaactctt
     8761 cttcaaaatt tgaaaataat tgagctaatt tcattatttg tttaattctt ctagtttttg
     8821 tgtttggtca taaagcacca aggcatccac aacttcgtcc aatttaccag acaaaatcgt
     8881 atctagtttt tggagggtca agccgatacg gtggtctgtg acacggtttt gagggaagtt
     8941 ataagttcgg atccgttctg aacggtcacc agtaccgatt gtcgacttac gctcagcgtc
     9001 ttgttcatcc tgagcaatct gagcaaagtg gtcagcgacg cgtgcacgga taatcttcat
     9061 agccttctca cggttcttct gctgggtacg ttcttcctgc atctcaacct tgatattggt
     9121 tggcaagtga acgatacgaa cggcagtcgc aaccttattg acgttctgtc caccagcacc
     9181 tgatgcgtgg taaatatcaa cacgaagatc ttttggatcg atatcgtatt ctacttcttc
     9241 gacttcaggc atgacaagaa ctgtggcagt tgaggtatga acacgacctt ggctttctgt
     9301 cacggggaca cgctgcacac ggtgggcacc tgattcgtac ttaagcttag agtatacaga
     9361 ctgacctgaa accatagcaa ccacttcttt aaacccaccg acaccattca tagaggcttc
     9421 catgacttca aagcgccagc cttgggcttc cgcatacttt tggtacatag ttagcaaatc
     9481 tccagcgaaa agtgccgctt cgtctccacc agctgctcca cggatttcaa ggatgatatt
     9541 cttgtcatcg tttggatcct ttggaaggag caaaattttc agtttttctt catattcttc
     9601 tttttcagcc ttggcatctt tgagttcttg cttggccatt tcttccaagt ccgcatctcc
     9661 gcctgattcc ttaatcatct cttcggcatc gacgatattt tgaaggactt gtttatactc
     9721 acggtaggct attacggtat cacgattgga agcttcttct tttgaaagct ccataaaacg
     9781 cttggtgtct gaaacgacat cagggtcact cagcaattct cctagttctt cataacggtc
     9841 ttctacagct tgtagttgat catagatgtt catttttctt cattctcctt attgatttca
     9901 ggggcaaaat aatgtttacg gcaaaccgag atataggttt cattaccacc aatttggatc
     9961 tgttctccat cgtaaacggg cagtccatcc tgtgttcgca acaccatggt cgcctttttc
    10021 ttgcaatact gacagatggt cttgatttcg tcaatcttgt ctgctaaaag caagagatat
    10081 ttggaacctt cgaacaattc attgcgaaag tcatttttca agccaaaagc catgacgggt
    10141 atgtctaact cgtccacaac acgagctagg tcataaacat ggtggcgttt gagaaactgg
    10201 gcttcatcga ccaacacaca gtaaggtttt tctggtaggc ctcggatata gccaaagata
    10261 tccgttgttt cctcaatcgc aagggcaggg cgtttcatgc caattcgact cgacacatag
    10321 ccaacaccgt cacgcgtatc cagagctgaa gtcataatca caacaccttt tccttgctcc
    10381 tcatagttat aggccacctt gagaatctcg attgtctttc cagagttcat ggtcccataa
    10441 cgataataca actgtgccat gtttcttgct tcacgtccat ttctaaattt ttgctacatt
    10501 ctagtatatc ataattttct taagctttaa acggcaaaat gtggtaaaat ggaagaaatc
    10561 aaaactagtg gaggaagcta ttatgccatt tgtacgcatc gatttatttg aaggacgcac
    10621 gctcgagcaa aagaaagctc ttgctaagga agtaacggaa gcagttgtcc gcaacactgg
    10681 agcccctcaa tctgctgtcc atgtcatcat caacgacatg ccagaaggaa cttacttccc
    10741 acaaggggaa atgcgtacta aataagctag cttaagcaga attgcttagg ctttttcaat
    10801 ctccaagtag cattcattga agaaatatcc taaatttgtt acaatttgaa aagaaacttg
    10861 gagaatttcc aagaaaagag ctattaatta aaggaaacat tatgattaca cgtgaatttg
    10921 ataccatcgc tgctatctct actccactag gtgaaggggc tattggtatt gtccgcctga
    10981 gcggaacaga cagttttgct attgcgcaaa agatttttaa aggaaaagac ttgaacaagg
    11041 ttgccagcca cactctcaac tacggtcaca ttattgatcc tctgactggt aaagtcatgg
    11101 acgaggttat ggttggggct atgaagtctc caaagacctt cactcgtgag gatattatcg
    11161 agattaacac ccacggtggt attgcggtga ccaatgagat tctccagcta gctatccgtg
    11221 aaggagctcg gttggcagaa cctggtgaat ttaccaaacg tgccttttta aacggtcgcg
    11281 tagatttgac acaggccgag gcggtgatgg atatcatccg cgccaagaca gacaaggcca
    11341 tgaacatcgc ggtcaaacaa cttgatggtt ccctttctga tcttatcaat aatatccgtc
    11401 aagaaatcct taacacactt gcccaagttg aggtcaatat cgactatcct gagtatgacg
    11461 atgttgagga agccactact gctgttgtcc gagagaagac aatggagttt gagcaattac
    11521 taaccaagct ccttaggaca gcacgtcgtg gtaaaatcct tcgtgaagga atttcaacgg
    11581 ctatcattgg acgtcccaac gttgggaaat caagccttct caacaacctc ttgcgtgagg
    11641 acaaggctat cgtaaccgat atcgctggga caacacgaga tgtcatcgaa gagtacgtca
    11701 acatcaatgg tgtacctctc aaattgattg atacagctgg tattcgtgaa acggatgata
    11761 tagtggaaca aatcggtgtt gagcgttcga aaaaagccct caaggaagcc aacttggttc
    11821 tactagtgct aaatgccagt gaaccactga ctgcgcaaga cagacaactt cttgaaatta
    11881 gccaagatac caatcgcatt attctactta ataaaaccga cctgccagaa acgattgaaa
    11941 cttcgaaact acctgaagac gttatccgta tttcagtcct taaaaaccaa aacatcgaca
    12001 agattgaaga gcgaatcaac aacctcttct ttgaaaatgc tggcttgatt gagcaagatg
    12061 ctacttactt gtcaaacgcc cgtcacattt ccctgattga aaaagcagtt gaaagcctac
    12121 aagccgttaa tcaaggtctt gagctgggga tgccagttga tttgcttcaa gttgacttga
    12181 ctcgtacttg ggaaatcctc ggagaaatca ctggggatgc tgctccagat gaactcatca
    12241 cccaactctt tagccaattc tgtttaggaa aataagaaaa atccatgatc cttcattcgg
    12301 tcatggattt tattgtcttt attagtaatc tggtcttaag acccctgtta cagttgcctt
    12361 agttgcttcg tagtcgccat ctacgacaac cttgataatg cgtttggcat cttcttctgg
    12421 tgctggaaca agaggtagac gagtgggtcc agcttcaaat cccatatagt taagaattgc
    12481 cttaactgga gcaggacttg gataagagaa gagagcatta accttaggaa tgaatttacg
    12541 ctgaattgct gcggctttct tcatatcgct ttctgcaatg gcagtaaaca tctcgtgcat
    12601 ttcatcccca tttgtatgag aggcaacaga aataacccca tccgccccaa ggttcatggc
    12661 atggaaagca tctccatcct cacctgtata aatcaagaac tcttcaggct tgtgctcaat
    12721 caagtaagcc atattagcca agctagtaca ttctttgaca ccgataatat ttggatggtc
    12781 agccaagcga agcatggttt ctggagtcaa ttcgacaact acacgccctg gaatgttata
    12841 gataataatt ggtaggtcag aagcatctgc aatagtctta aagtgctgat acatcccttc
    12901 ttgagaaggt ttgttgtagt aaggaacaat agcaagccca gctgcgaaac caccaaattc
    12961 cgctacttct ttgacaaact caatagagtc acgcgtatca ttagtaccta cacccgcaat
    13021 caaaggaacg cgtccattga caaccttttg tacagccgca aacaactcca actcctcatc
    13081 gtgggtcaaa gttggactct cagcagtcgt tcctgcgaga agaattccat ccgtatgatg
    13141 ggccaataaa tgctcaatca aggctggaat agcatcaaag ttaatagaac catcctcatg
    13201 aaagggggta ataaaggctg taatgatttt acattctttt aaatcttgat aagacatgaa
    13261 aacacctctc tatttcaaag aaggagttca tctgaactcc taaacgatat gactatttta
    13321 attcaaattt caattcggct gttggacgaa ccaatccacg ttcatgaaga gtttcagcaa
    13381 tctgaactga gttccaagca gcacctttga gaaggttatc tgaaacaacc cacatgtgaa
    13441 ttcctttttc tgcatccaag tctttacgga tacgaccaac aaaggtatca cgcgaaccaa
    13501 ctgcattgat agcttgagga tagatttgat gagctacatc atcttcaaga acagcacctg
    13561 ggaaggctgc gatagctgct tttacttctt cgattggagc cacttctttt gtttcgatat
    13621 aaacagactc agagtgagct gacaagactg gaatacgcac acatgttgca gatactgcaa
    13681 tgctatcatc ttccataatt ttcttagttt ccttggtcat cttcatctct tcgtacgtgt
    13741 aatcattatc agtgaaaaca tcaatttgtg gaagagcgtt aaaggcgata ggataatgtt
    13801 tcttgtcacc acctgaaggc aaaatttccg catgcaaatc acatggtttc acaccatcat
    13861 tcaagacttc acgaagttca cgttgtgtct caagaattgc tcccatacca gcacctgaaa
    13921 cggcttgata agttgaaaca atgatacggt ccaagcccca tttttggcga accggctcaa
    13981 gagccaccat catttggatt gttgaacaat tagggcaggc aatgattccg ttgtgggcat
    14041 caagtgcatg agcattgacc tctggaacaa ccaaaggaac atctggattt tgacggaaat
    14101 aagatgtatt atccactact accacgccag cttttactgc gtatggtgca tacttagctg
    14161 atgtagaact acctgctgaa aagagagcaa tatcaactcc ttcaaaagct gtttcagtcg
    14221 tttcttcaat tgtaatatct tgatctttaa atttcaatga cttacctgct gaacgtgcag
    14281 aagcaaggta acggatttta tcgattggaa gtgttgattc ttccaacatt tttatcatct
    14341 gagcaccgac agcacctgtc gcgccgacta cagcaactgt atatcccata aataacctct
    14401 ttcggaattt tctaaaaatt tctataatag aagtattata ctactttttc catgaattgc
    14461 aaagcttttt catcattttt tggaaaataa aaatcccagt cgctagacta gggactaaga
    14521 ggcatcttca tgtgatgagc aggttcacac aactcatcaa ggtccgctcc tgcgttatga
    14581 cctccttatg ctcaatagta gtccgaagac tacctatcga ctcatcgcat attctatatt
    14641 actagagata cttgattttg tcaataagaa attttttcga tcgagtataa caaaaaactc
    14701 tcgtattcaa gagtttccct atacatatca tgctaattgc cgggatcgaa ccggcgacct
    14761 catccttacc atggatgcgc tctgccaact gagctaaatc agcttactta agaagtatac
    14821 tatattcaga aaaacttgtc aagtttcctt agaaattttc cataggaaaa agccaggtaa
    14881 gaactaccta gcttatcttc ttctatttac ttaaatcaat tcgacgacca attttaccaa
    14941 taataatcgc cccaataatc aatgaagcaa attcaccgat tcctgttgta aaccaagtaa
    15001 ggaagaatgg taattctgct acaatattta actctgcagc gatagtaaac atggaaattg
    15061 aaaagaggat tgaaaagaag aaatgatctt ttcgaatcaa tccgttaaag aggtaatcct
    15121 tgctatattt tgcaaaaagc caaacaccta aactgaggaa tactaaggtt gaaccaccac
    15181 caacaaagac atctagcagt ccgaagctaa agaaattagc aatcatacaa ccgatggtaa
    15241 ctccgatgat gtacttagga ttgtaaaaag ccataaagtt catcatttct gaaatacgaa
    15301 actgataagc accatagctg atggcattta ggggcggtgt gacagtcaaa acgacgtaga
    15361 tagcagcgac gattgcaata tctgcaatat cacgaatagt taattttttc atcttttctc
    15421 ctttggcggt tgcccgcgtg taatatgctt ggtgaaagaa gctaagcacc aagggttgct
    15481 ttattcaacc ctactagtat agcacaatag cctgctttat gctatactta acacatgaaa
    15541 attcctattc aaaaattttt taacaatgaa atcttagcct atctcttttt tggccttgcg
    15601 acaactctag tttcgattct ctcacgacta gtcatttatc aactaagtca tcaggaactc
    15661 ctggctactg ccctagcaaa tattatcggt atcctatttg cctttatcac aaatgatacg
    15721 attgtattta agcaaaaaaa gaaaaataga cttatccgtt tagtgaaatt ttctcttgct
    15781 cgcctttcta cttttctgtt agacttgttt ttaacttttc tctttgtaac ccaatttcct
    15841 cacattatag ggcaattcgt aactgaaaac attgataaga taaatgctat tgaaacaata
    15901 attgcacaaa tattcattat tatcatcaat tatatattga gcaaggccta tatttttaga
    15961 aaataatgct ccatttatga gccttttaat tgccttctat aatgatttag gatacaatga
    16021 aacacgaaac attgaaagga aaacagtatg ttaaaaaatc taaaatcgtt cttgcttcga
    16081 ggaaatgtta ttgaccttgc tgtcggtgtt gtaattgcct ctgcttttgg tgctatcgtt
    16141 acttcacttg taaacgacat tatcactcct cttattttaa atcccgcttt gaaagctgct
    16201 aaagttgaac gtatcgctca actttcttgg catggagtcg gctatggtaa cttcttaagt
    16261 gctattatca attttatctt tgtgggtacc gccctcttct ttattatcaa gggcattgaa
    16321 aaagcacaga aactgactgg cataaagaaa gaaaaaactg ccgaaaaaaa accaaccgaa
    16381 ttggaagtcc ttcaagaaat aaaagctctc cttgagaaaa aataatcgat taaaaggatc
    16441 tagcgcaaag ctaaatcctt ttttctttac tctttgggta acttgcctgc tttttcaagc
    16501 atttttttga tcagataagg catcttaaca ttctcccgcc cttttttctc aatcagtttt
    16561 ttcacaaatt ctggcatttg taaatcttca gattcagcta aaatgttttt cgtctcgtct
    16621 gaaggaacta actcatcaaa ggtctcttct tctgggagaa attccttaaa ctcttgattt
    16681 tcattgactc taactgtatt gaccaaagca tcaatcaaac gagaatctgt atttggcatt
    16741 ggtggacgat ggtagtttac cccaaattcc tggcataagt cataacattc tacatcattg
    16801 tcaaacaaga cctcaatatg ctcactgatg aaactaatag gtacaaaaat ataatggtct
    16861 ggatgttctg tctgttctct gagatactcc aaaacatctg gtttaatcca tggaatacca
    16921 atatcactct cactctgcca agtgttggta tattgttctg aactcaagcc cagtttttca
    16981 gcgactagct tgctattttc gaaaatttgg tctatataag gatcaccaaa gtccaaggca
    17041 aaaatgggca cactatgggc tgaaaagatg actttaaagc tgtcttgctt tacttcttct
    17101 tttaaaatct tagcaatttc atctgcccaa tagtttaaga gcacttcttc ctgataccag
    17161 tccttaatga ccaaaaattg aatttgtttg ctttctaaaa atttttcata gcccatgaca
    17221 gagtagaaag aataatgggg ctccaaaatc aagcagatac attcttcaac accatcagtt
    17281 tccatctgct tgatcacgtc tggaataaag ggacgagaaa atttgttggc aaagtagata
    17341 ccgtactcct cccctaaacg ttctttgact aaattgacct cctcacgtgt aatccgctgc
    17401 aaaggcgtcc caccgatacg aatatagttg tcatagagag tctggatttc gtggtcttgg
    17461 ggtctaactc cacgacgaat gtttgtaaag aattctgcta ctccttcaaa actaatctcc
    17521 tctggagatc caaaggtcat cattaatatg gcttttttca tgttatcatc cttatgatat
    17581 tattttctct atccattcta tcatgaaaga aaaaaatagt aaacgtttcc tacgtctact
    17641 atttcttttg ctcttttgtt tttttatctc gtcttataat taggatgata aggacaagaa
    17701 gtaagaggac gataataagg agtagaaagg ctagaatgta aatccaatga ctgggaattc
    17761 cgtgatcgaa ataccgtgtt cctactgctt ccccactttc atcctgtgct actttcaccg
    17821 gtgcatccgt tattttagct aaaaggatat gtcgtccatt agttatgtta aggaaacatc
    17881 tcgcttgtac ttggctttac ttagcagata ttgatagtaa gcctggtgtt cctcatcctt
    17941 ggaactcaag gttcgataaa tctccgtgtc ataggcatcc acttctaaaa tcccaaaaat
    18001 ttcaagacca cgttctcgac cattgtagta gatagaaccg tagcgatgct ggtcgaagaa
    18061 ttccttatca gcaaatttct taatctcacc gaacatagcc cctgaagcca tagagtgtcc
    18121 gtagatgatc gtattaaaat cagtaaagtt gggattaaag cgatagtcga gaaaaattga
    18181 tcccattatc gtataatcgc caaaggcatt gcggtcaagg tatttgacat tatcctgact
    18241 ttgtagaagc ggatagtcta ccttggtccc atcaaggcta atccaaccgc aaacatcttt
    18301 gttcaagtgc tgtagggttt caaaacctgt gctcgaagca tccttttcaa taggattata
    18361 gtgatcatag tcacttgctt ctactactct ctgccaacta aaaagagcta caaaaacaca
    18421 gaagaatacc agaaatacgt ttttaaatcg cgctcttttt tgaaccgtca tcagcactcc
    18481 tttctatgcg agatttctta aaaggaaagg aggcgggaca aaaccgcctc ttttctattc
    18541 acccttagtc agtaaaaggg tctgcttctt gcattttttt tactctctct tctacgacgt
    18601 aggatcaagt agaggacaag gagtaagaaa atcaagagca agaacaatac aaacaagata
    18661 aggtaccaaa tccatagtgg gattgatgag aggaaacgtc caagagacga gtcatcaaag
    18721 acactgtatg gaaatggttt tgattttttt gtatggaaag tatttttagg gacggtgtct
    18781 gtaatctttg cgactacgat atgacgacca ttggtcacat cgagaaaaca ggtactgagt
    18841 aggatgatac gatcactcgg tgagagtgag atatcccgct tgtgcatggc gactgagagc
    18901 aggtggtcta gataggcctg ctggcggtcc tcaccctgta ttcctggatc atagatgtta
    18961 aagtcatagg catcaacctc caacatctca aagatctcga gccctttttc cacaccattg
    19021 tagtatatac taccgtaacg atgctggtca aaaaattcct gatcagcaaa cttagccaca
    19081 tcaccaaaca tgaccccatt ttctacgtgg tgcccgtaga taatggtatt aaagtcttcg
    19141 aacttaggat tatttcgtgc atcgagaaag atagcgcctg tcgctgcaaa ctcacctttg
    19201 gagtccttgt tgagatactt ttcattgtcc ttggcttgga ctaagggata gtcgatattg
    19261 gtgccataga cattgatcca accgaggact tcgggattga gttcttggag tttgctgaag
    19321 cctgaaaaac tggccagctc atcctgttgc gtcgtgacag gtcgataagc ttcgtactca
    19381 cttgacgaag ctaaggagta gacttggttg gaatcccaaa gggcgtagaa accaaagaga
    19441 aagatcaaag ctgcaaaaat gagcagcaag tgattgatca ccccatccac agcaagaacg
    19501 agacgcctag acagactctt ttttggtttc tcacgcttgg ttttcatcat ctgcccccag
    19561 ccgtttaggc ttctctacgt tttttagaca agacaacaaa tccagccaaa ccaagaccaa
    19621 tcatcaaaat gaatggaagg ttatcaatca agagaccagt aggagtaacg tcgtcgattt
    19681 tgttagtaac tttgttgtcg tttttctttt cacctatgag gatatctgtt aatgtaaaat
    19741 ctacattttt ttgtccttga gatgtttgtt ctgcaccatt tgtagtataa attgatgaat
    19801 ctgtataacc tgctactcca gtttcagtta ctgtatattt agaacctgct ggtagttgtt
    19861 caactgtaaa tgtttcacca tctttaagag taaaattgta ctcaactgta tctgctgggg
    19921 tcactactgc caacacctct gacttagatc catgttttac tataatattc gtaacagggg
    19981 tttctgctgt tttatttgta cttggaagtt gaatcttgat atggaattgg aaatctcttg
    20041 ttttatcccc tgaagctcca tcaacttttt tagtaattgt taatgctttc gcattggggt
    20101 ctacttgact cggatcatca ggattaggct ccttacttgc gtctttggta tatgtgttat
    20161 caaataaatt ataaacgcct tgttcacttg gttctacttt aggggcgtta gatttattat
    20221 tttccttaaa gtatactgat gaaatataga caccaccatt tactttattc ttaacaataa
    20281 cgtgcatttc atatgtacgt ttgtcgtatc tcatggcatc aattgtttta ccatttttgg
    20341 taatcgcctg ccatccagtt tttgcttccg ctacatcata aacgtattca ccagcatggc
    20401 cataagcaac tcccgaaaaa atgtcttctg ttgctttttg aatcttatca gcagttgggt
    20461 gatcattttt attgtattct acatttctat ctggaattgc aactccggtt tgatatggcg
    20521 caccgttaga agattgtcct gttttttctg taaacttaaa tgtaaatgtc gctgttggtg
    20581 ttgttattcc ctctgcaata ttcaacgttt tattaatctt cacaacagca ttattttcac
    20641 catttgcttt ggttattgct gaattatcat ccgcaaaagt cgggacacct ctgaatacgg
    20701 acaaagcggt tatagctgca atgcttgcag tgaatagctt tttaaagaat gtttttttca
    20761 tgattatttc ttttgttttt ttattttctt ttttcattat gatcatcaaa agcctcgtct
    20821 tctaatgact gttacaactt tgccttgagt atcctgtata gggatggttc caaaagcacg
    20881 actgtctaca gccgttgttc gattgtcccc gaggacaaaa agttgtcctg ctgggacttt
    20941 catcgggaag gttgcccctt ccttatagag cagcgtttct ttgtagatat cttgctcttg
    21001 ttgaggagaa ccgttgatga tgagaccatt ttcgtttata tcgattgtac ttccttcggt
    21061 tgctatgaca cgcgccactc tttccttacc tttataacta tagactagca aatcaccaat
    21121 cgaatagcgt ttatccaacc tataatagac gaccaagtcg ccatctttta aggcgggctt
    21181 catgccatcg tcattgtatc ttaataatcc aaagacgaaa aagtaaagga tggctagtac
    21241 aaatccaacc atcagaagtt tactgactaa gtaaaggata tctccccaga cactcggcgg
    21301 agacttatcc cgattgactt gtgttactgg tttcttatgt ttctttttaa gcagcatcac
    21361 ttcctcctat tcaccacaag gtgaccaagt aagatgataa agaagatccc tcctagagga
    21421 agaacgactc caagcacgag agtgctactc aagaagtcaa ttcccgtttc aggtacgctg
    21481 tttttattat tcgtgactgt cgcagactta tttgcatcaa gagttccttc ttttttatca
    21541 taggacacct gatacccacg agacgaactt gcttcttctt cgatactata acgagcattt
    21601 agaggaaggt cgagaatctt gatggtttta tctttcttta gatcaactgt cgccttacca
    21661 ttggtgaatt gtagcgttgt tttttgatta tttactattg cactatacga tccattcaat
    21721 ggtttatttt gcgcatcctt tacgttaatg gtaatcttaa aggattgcag aagattagca
    21781 aaagcacctg taactttttt tgtgactgaa agtgtcggaa gtctgtggtt ggtgacgatt
    21841 gtttgaacat cctctgacaa agtcccctct tgtttttcat aggtgacatg atatcccgtt
    21901 ctagagttct ctacttcttc caccttatag tgggtatcac gaggcagata actgagggca
    21961 gtttcctgac tatttttgag agaaatctgt gctttcccgt tagtcactgt tgtattccca
    22021 aacttgccgt taatcggctt gccatcctta tctgaaaagg taacattaat tgtgaaagat
    22081 ttattaggtg attgttcacc ttctaggatt ttctttacgg tgagacttgg aagctgtcgg
    22141 taaataactt tgaaggatgg tttctctttc gtacctgtct tttgaacttc gactgtgtat
    22201 cccggagcgt ctggtgcttt cagttcgtag tcatatccct tagctacctt tgcaaaacta
    22261 gtattttcag taaaactatt cgtttgaact ggactagtga cctttcgata ggaatccttt
    22321 atacttccat ctttcttttg gtttaagttt aattcgacac taggtggacg atttgctgat
    22381 ggatttgatt ttccatccac acctttccac tcaatctcta caggaaccgt caatggatct
    22441 gacggcttga aagtgggctt ctcagaatag tcttttattt tggtacgtga ggtcccaata
    22501 ccataggaat aggcaacttt tgcccccttg ttggagtaga gtttagtagc atcggcttct
    22561 tcggcatcat cactagtaag tgtcttatca cccgcaatcg catcaaatgc ctcttggcta
    22621 gaggtgacag taaacttgag aacgtacttg gcgtcatcct ccaaagtgta actcggatta
    22681 aacttggctg ttacttccac taatccctgt tcgttcttct tagtttcaat cgtaacttga
    22741 ttgtcattca gttcttgctc gttaccatcc ttgattttga cgacacgaag gtgtgaagca
    22801 tcaccaggta acagctgcac gtacttggac aagacatctg atatagttac atggtgtacg
    22861 cctagaggta gaattttatc tgtgatttct ttgaattttt gttgcaagtc attttcatta
    22921 ttggcagaaa atatttcgtt ggggattcca gaattataac cactgatata tcctttaaga
    22981 gatgtgatac tatcgtttga acctatatat ctgaatttga ttgaataaaa gccatctatt
    23041 tttggagcta ttaaactaga tatattcagc attcctctct ctaaaccaga ggtaaaccaa
    23101 tatggagtat caatcatacg tcccacattt ggattagaat aatagttata tattgttctc
    23161 ccactattag actcgtaata catattagct tcgccatctg acagcagaat aactactttt
    23221 tttgccccag cccttgcact ttgcaacaat ctttgagctt cagttaaccc agcatttata
    23281 tttgtcccag tgccaatgcc cgcgtccata ccataagaac gactagagtc agcaattgtt
    23341 aacgaactaa caatattttt tgagctatta gaatccgtgc tccaagatac taaagtttta
    23401 gcatcatcat atgaagagat atttgaatac caaccagctc aatttggcca agttccatat
    23461 tgataactca gataatattc attataataa cggtcattat actgattatc aatctttccg
    23521 gcaaaaccaa ctatagacag acggttttgg gaattagaaa gaattgtatc aatgagcccc
    23581 tgacgaccat tcacaccttt cagtgtatgt tttagtgctt caatcctact aatgtatcgt
    23641 ctactagagt aagaccacac atctcgctct tccatactcc ctgaaagatc ggcaaccaag
    23701 actacatcca agggttctga ctgggtctcc gttcccagtt tagatgtgat atccagtgac
    23761 aactcatact ggtctttttg ccctgatatc ggagtaatcg ttttgtgcag agttgtctga
    23821 ggttcagtta ttgaatctgc acgtaaagaa ttaattggtg cacacaaaaa aagaatagcg
    23881 agaaagataa ctaaactact ggtaaaatat tgaatcttct taaaatcata ttgaacgttc
    23941 atgctatctc ccttgctaga atatgtttta atctgatact atataaatct gagacttcca
    24001 taagataatt gtataataat attaacttcg tgtcaattaa tgtgtttgaa aacatgttat
    24061 tataaattac aaaaaagtat gattcttctt tgtttttcaa ctttttctgt ggtaaaatgg
    24121 aaaaaatagt aaaaaatttc ataaaataaa gatgcatata tttattctaa tcgctcgaaa
    24181 atttcttttg aaagtcttct gaatttgaat tattatattt tttcaaaatt cataaaattt
    24241 ctcaaatcat atttttccga agttttgtat tctattttat gataaaaaag gagttttttt
    24301 atgacttatc caaatctctt ggaccgcttc ttaacctacg ttaaggtcaa cacgcgctct
    24361 gatgaacact ctactactac tccaagtaca cagagtcagg ttgacttcgc aacaaatgtc
    24421 ctaattcctg aaatgaaacg tgttggactg caaaatgttt actatctacc gaatggtttt
    24481 gctattggaa ccttgccagc caacgatccg tctttaacac gtaagattgg ttttatatcg
    24541 cacatggata ctgctgattt taatgctgaa ggagtcaatc cacaggtaat tgaaaactac
    24601 gatggtggtg tgattgaact agggaattct ggtttcaaac tcgatccagc cgacttcaag
    24661 agtcttgaaa aatatccaag acaaacactc atcacaacag atggaacaac cttgctaggt
    24721 gctgatgaca agtcaggaat tgctgaaatt atgacagcca ttgaatatct aactgctcat
    24781 cctgaaatta agcactgtga gattcgtgtt ggttttggtc cagatgaaga aatcggtgtt
    24841 ggtgccaata aatttgatgc agaagatttt gatgtggatt ttgcctacac tgttgatggt
    24901 ggtccactag gtgaacttca gtacgagact ttctcagccg ctggtgctga attgcatttc
    24961 caaggccgta atgtccaccc tggtactgcc aaagggcaga tggtcaatgc ccttcagcta
    25021 gcaattgatt ttcataatca acttccagaa aatgaccgac ctgagttaac tgaaggttac
    25081 caaggttttt accatctaat ggatgtgaca ggtagtgttg aggaggtgcg tgcaagctac
    25141 atcattcgtg attttgaaaa agatgccttt gaagcgcgta aagcatccat gcaatctatc
    25201 gctgataaga tgaatgaaga acttgggagc gaccgtgtca ctctcaactt gacagaccag
    25261 tactacaata tgaaagaagt cattgaaaaa gatatgactc caattaccat tgctaaagcc
    25321 gttatggaag atctaggtat cacgcctatt atcgaaccaa tccggggtgg aacagacggc
    25381 tctaagattt cctttatggg aatcccaact ccgaatatct ttgcaggtgg cgaaaatatg
    25441 cacggacgtt ttgaatacgt tagccttcag actatggaac gtgcagttga taccatcatt
    25501 ggcatcgtag cttataaagg ctaaaaagac gaggtggctc agctacttcg cctttctttt
    25561 tattctactg gtttttcttg atttccagta gttgtagaag attctgttgt ttcattttct
    25621 gaagttgatt cagcaggttt agaatctctt gtattgcttg gtttgttttc gtcgctagca
    25681 gtttcaatgt tagattctgc agttgcgttt ggttggttct cagcactggt gttatcacta
    25741 tttgcttcag catttcttgc tggacttgtt tcttcacttg cgctaacttt tgactggatt
    25801 tgatgattca aaactagaat agcttttgtc gattcaagta aagctgtttt gtctttacta
    25861 ttagcagaaa gttgatctaa taatgcatcc accttatcaa agtccgcatc agatccatta
    25921 ttactttcta aataagagtg aagcgacatg agaatatcgt agagtttttg atagagtaca
    25981 agtgtctgag aatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga
    26041 gtcaaaacat cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg
    26101 gcagctttta gattttctac ttcttctgcc aaggattgtc tgattccttc ttcatggatt
    26161 tgttccaaga gttgatttgc cttgttcaaa agactttcta cttcttcctt gctatctgtc
    26221 gcagattatt ggttgctatc taccatgtac tcctaaaaca ggagagttat aatccaagat
    26281 tacaaggcct tacagaaata agaaatccag ataagacaat gttcgtccaa gacgctattc
    26341 gcttcgcaca gcagcacgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct
    26401 ctttttagtg tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa
    26461 actttcagta aactgaccta aagctaactc aatctgtctt tgtagatgct tctgctatca
    26521 gctagaagtt gatctacttt tgccaagact gccttctcat caaaagttcc aggttgatag
    26581 ttggattgca gggatggaat cttgtttttc aaagccgctt catatccctt agtttgaacc
    26641 ttgatgtagt gattgtggtc gccatgagga atcacaaaac cttctgaatc ttcacttata
    26701 attcgattgg catcaaaaac cataaccatc ttcttcctca tggtggacat gtagtgacgg
    26761 attacttaat acagaactag aagaacttcc tacctcttcc gtgttagagt gtgatggggg
    26821 attgttaaga gatgacttag gaatatagtg atagtgttcc ccatgtctta ctatataagc
    26881 atcacctata tctctgacaa tatcattagg gttaaagaca tatgtggctg ctaattcacc
    26941 tgccgacaag tcactctcag gaatgaaatg atagtgacca ccatgtggta ctattagtag
    27001 attgaaatag aatatgagca aattgataag gggattttaa agtaatttct aacaatgatt
    27061 tagaaactat gatgtgctat tctaaattca actcactata tataaccatc atcggtagta
    27121 taacgtccct gtaattttgc tacagatact tctgcactag ctcctttatc gtctttacca
    27181 tgttcttgtt tttggcgatt gatttcatct tttgttcgta cattttctgc atgagcttga
    27241 tctttaaggt aaacataata ctttccatct accttaataa tatatcctcc cttaacctaa
    27301 ctgacaatat cttgatcttt cggctgatag ttgggggctt tcattaatgg ctcttcacta
    27361 aagagcgcat caaaaggaac tttaccatta tagtagtgat aatgatcgcc atgagaagtt
    27421 acataacctt gatctgtaat cttaataaca atttgttttg cttgaattcc ttctttttga
    27481 ctaacctagt ctggagtcaa attttcagtc ttcttagtgt ctttattact gtttacatat
    27541 gaaacacgat ttttatctgt attggcctgt tagctatgtt ggttcagagc ataaacacac
    27601 agacttaagg aaaggataac aacagatcca gctgctatat atttcttttt aaatttcata
    27661 attacctcat ttctataatt atttatatga tgtcttcatt attaaatgat tatagaaatt
    27721 aattaaccaa ttaattaact agtaaatatt ccacctcttt ttaagttgta tgtcaagaaa
    27781 ttttatatat taataataaa atgaaattct cccaaagtca gagttttatt tctaactttt
    27841 gagagaactt catttttgat tcagactttt tctactgcta ttccttacgc tatgagatca
    27901 gataaattct tttttatcac ttctccactt ggcaatctta attcaatcgt tccatccata
    27961 ttgaatataa cactatctaa gcctaatccg taactagctg taaatttttc taatttttct
    28021 tgtacaggat ctactgctgg agcttcctct aatgctggat ctaacatagg gtcactcccc
    28081 acattccctt ctggattcaa cattccatta tccgttgagt tttctggttt tacaggtttt
    28141 tcgtttggtg cctctggtaa agaatctgct ggtttatttt ctgttggttg gttctcaact
    28201 gttccagtag atacttttcc attttcagat ggtttatttt caccatttcc ttgaggtgct
    28261 tctcctgtaa aatctgccat attcttttta atgacttctc ccgatggtaa atataattca
    28321 attgttccgt ccatattaaa caagacattt tctagcttca tcccataact ttcagcaaat
    28381 tttgctactt tttcttgtac aggatccact gtaggaactt cttctaacgt tgaattacta
    28441 gtactattcc cagtttcaga aagtttttct ttttctacct tctcactagt ctttggttct
    28501 tctacctttt catcaagttt tgagttttct tgtgctttat tccttttaaa ttgtggtaga
    28561 atacttggtt tatcagtttg attttctttt tccaagatag gtacttccac aatataagtc
    28621 gattgattgt ccaaataagc atttgccatg aaggttacag gaattttatt tccggccgtt
    28681 ctggttgttc cttggtttaa tttcggaatc ggtaatttga tttcaccaac tttatagtta
    28741 ttttctaaat aagcatttcc atgaaattca tcaaacactc tgactaaagc atcagttcct
    28801 ttaggcactg caaattgagg gttcactctt aaataagtat cccctgcatg gaaaggatag
    28861 aaaatcgttt gactggccat tttgtaagct aaagaggttg gaactgtaaa tgtaccatca
    28921 taacttactt ctggataatc ttttgaagcg atagtatact taaatgtttg tcctggtaaa
    28981 taaggttgat ctaattcaaa gtttgcaata ttccctactc cttctccaaa tactttacca
    29041 gatactttct ccaatacttt tccatctggt gttattaatt ttactagcat attgatacct
    29101 aattttttct ccaattcagg cggaaaacta aaagaaacgc gtttttgacc attggctaga
    29161 gtaaagtttt gattattaaa cgtactattt tttaacaaat taacaacatt cgttaattct
    29221 tctccagtat aaactttatt cccttctttt ttagcaactc cttcttcggg tttaaacagt
    29281 tcatagttac tgtgagaatg accaattcca accggtttat gttcatcaat cggatctgca
    29341 tgatggtgat ctccatgcgg ataaataatc gcattttttt ctttattcac gacaatactt
    29401 tcacgtttga caccatattg tttcataatg ccagcaattt tttcttcgat ttttttatct
    29461 aaatctttca tttctttggc attacttgga taatcctgtt catgagatga caaagaatct
    29521 aatccattat gactagtttt aacttcctct aaatgttttt gcgcagcctt aatttgctct
    29581 tctgtcaagt ccttcttgaa gaaataatga ttgtggtctc cgtgactcat gacaaaacct
    29641 gattcatctt cagcgataat acgattagca tcaaatccgt atccatcttc ttcatgtttc
    29701 tcatgtgaag ttcctggatt gattggaaga gatggagaag gtgttgctag actattgttt
    29761 ggaagagtcg gttgcccaat ttgatttgat tttggaatgt aatggaaatg atcaccatgt
    29821 cttacaatat aagctgtagc cgtttcttca acgatatctt ttggattaaa aatataacca
    29881 tcagatgctg aagagagctc cttacttgtc gttaaagaag aaggattgct tgaaagactg
    29941 cctagactag acactacttc attaggtttt gcatttgtag aaactgtaga accagttcca
    30001 ctgataggca ccattctggc aatcttttct tctaaggcag aaagcttgct gtaaggaata
    30061 aagtggtaat ggtcgccatg cggaatcgca actccatttg gtgtacgact gataatctta
    30121 gcagggtcaa agaccaggcc atctgattca ctgtaacgtt gggcgctagg tgaatcatag
    30181 agttccttca aaagactctg gagattttca gatttatttg ctggcttgct agttgatcct
    30241 tttgctacag attgcgtgtt attgtcacta gctgttgaag aatagcttaa ctgactcggt
    30301 tgcatatttt ttccagccag atgtgcttta gctgctgcta attcactagc agataaatcg
    30361 cttttgggaa tgtagtgata gtgacctcca tgaggaacga tataagcatt acccgtatct
    30421 tcgataatat cagctggatt aaagacataa ccatcatttg tcgtatatcg tccctgagac
    30481 cttgctacag caacattaga gttaaccttc tcattatctt tgacatgttc ttgtttttga
    30541 cgattgattt catctttagt tcgaacatta tcagcatgag ctgcatcttt caggtagaca
    30601 taatattttc catcgacctt gatgatataa ccacccttga cttcattgac aatatcagcg
    30661 tctttaagtt gatagtttgg atccttcatc aagagttctt cactaaagag ggcatcataa
    30721 ggaactttcc cattatagta atgatagtgg tcaccgtgtg acgttacata gccctgatct
    30781 gtaattttga ttacaatttg ctcggcctga attccttctt tctggctaat ctggtctggt
    30841 gtcaagtttt cacttttctg acttgactgg ctgccatcta cataagagac acgattattg
    30901 tccttatttt cctgcgaacg atgctggttt agtgcatagg cacatagact caaggatacg
    30961 ataacagctg atccagctgc tatatatttt ttactgaatt tcataaatcc ctcattt
//
LOCUS       DAVBCR010000022        29035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCR010000022 DAVBCR010000000
VERSION     DAVBCR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.022"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            650..2803
                     /locus_tag="VQ508_000878"
     CDS             650..2803
                     /locus_tag="VQ508_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2489898.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VQ508_000879"
     CDS             2816..4165
                     /locus_tag="VQ508_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2489899.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VQ508_000880"
     CDS             4335..5042
                     /locus_tag="VQ508_000880"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2489900.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5244..8969
                     /locus_tag="VQ508_000881"
     CDS             5244..8969
                     /locus_tag="VQ508_000881"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2489901.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VQ508_000882"
     CDS             9062..10504
                     /locus_tag="VQ508_000882"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2489902.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10541..11563
                     /locus_tag="VQ508_000883"
     CDS             10541..11563
                     /locus_tag="VQ508_000883"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2489903.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11560..12105
                     /gene="purN"
                     /locus_tag="VQ508_000884"
     CDS             11560..12105
                     /gene="purN"
                     /locus_tag="VQ508_000884"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2489904.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12189..12698
                     /locus_tag="VQ508_000885"
     CDS             12189..12698
                     /locus_tag="VQ508_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2489905.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12723..14270
                     /gene="purH"
                     /locus_tag="VQ508_000886"
     CDS             12723..14270
                     /gene="purH"
                     /locus_tag="VQ508_000886"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2489906.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14392..15654
                     /gene="purD"
                     /locus_tag="VQ508_000887"
     CDS             14392..15654
                     /gene="purD"
                     /locus_tag="VQ508_000887"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2489907.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16057..16545
                     /gene="purE"
                     /locus_tag="VQ508_000888"
     CDS             16057..16545
                     /gene="purE"
                     /locus_tag="VQ508_000888"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2489908.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16532..17623
                     /gene="purK"
                     /locus_tag="VQ508_000889"
     CDS             16532..17623
                     /gene="purK"
                     /locus_tag="VQ508_000889"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2489909.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17633..17860
                     /locus_tag="VQ508_000890"
     CDS             17633..17860
                     /locus_tag="VQ508_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2489910.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17923..19221
                     /gene="purB"
                     /locus_tag="VQ508_000891"
     CDS             17923..19221
                     /gene="purB"
                     /locus_tag="VQ508_000891"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2489911.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19276..23214)
                     /gene="strH"
                     /locus_tag="VQ508_000892"
     CDS             complement(19276..23214)
                     /gene="strH"
                     /locus_tag="VQ508_000892"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2489912.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23529..24245)
                     /locus_tag="VQ508_000893"
     CDS             complement(23529..24245)
                     /locus_tag="VQ508_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2489913.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24593..26380
                     /locus_tag="VQ508_000894"
     CDS             24593..26380
                     /locus_tag="VQ508_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2489914.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26377..26853
                     /locus_tag="VQ508_000895"
     CDS             26377..26853
                     /locus_tag="VQ508_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2489915.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26881..27786
                     /locus_tag="VQ508_000896"
     CDS             26881..27786
                     /locus_tag="VQ508_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2489916.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27773..28588
                     /locus_tag="VQ508_000897"
     CDS             27773..28588
                     /locus_tag="VQ508_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2489917.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28595..28999
                     /locus_tag="VQ508_000898"
     CDS             28595..28999
                     /locus_tag="VQ508_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2489918.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         7953 a         5709 c         6837 g         8536 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcaggga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagccttat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccggga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tggtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt atcagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catggctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggtg gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggaggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgctcgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaaccc agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat ctcttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaagcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctatgg aaacaggtgc
     3421 ttccttggcc agtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacatttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat cttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaaattac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcctat ctaagttcaa aagatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttatctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt taaaaaacat ctgctattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcagatt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgcg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg ggtgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggac gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caacggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa cagatgttta cgaaattgtc
     5161 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc agggaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg actttctttg
     5761 aaagctatac agcagaagac tttgctcgct acaaggccga gcaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggagt taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa cggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggatgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaatg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
     6421 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggcg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggata ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtc taaggctcgt ttctcttacc aagaatattt cgagcggatg gacaagcaag
     7501 cagagcgttt cggtcagcca gtagctgctc ttctaggttc tattgaagca caaattcagc
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc
     7801 ataaagtgac gtctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggcttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 catacggaaa ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca
     8581 accaacacgt gaccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
     8641 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
     8881 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccagccaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc agcccttcct
     9361 cttccgtttt cacgatatgc agtttggctt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgtagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc
     9661 ggtggttgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatact cagttggcga tttgttctat ggagtatatc tactttgctc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    10141 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata
    10561 tgcccagtcg ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca
    10621 cgtggcccgt acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt
    10681 tgacctttcc aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg
    10741 aaccaagctc atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt
    10801 ggccatgtgt gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta
    10861 cgtagcgaca gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga
    10921 aggttgtgtg caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat
    10981 gtacggcgaa gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca
    11041 aatcattgac ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg
    11101 gattcactca aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga
    11161 agtcctacca gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat
    11221 ctatgtcaag gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat
    11281 cacaggtggt ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga
    11341 aattgatgaa agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca
    11401 gattaaacac gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt
    11461 cagccctgaa aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg
    11521 tcgcatcgtc aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt
    11581 gcctctggta atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc
    11641 ttttcagacc atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc
    11701 tatgcttttg aactcaagga gtttgagagc aaggcagact acgaagcagc ccttgtcgaa
    11761 ctcttggaag aacaccagat tgccttggtt tgcctagcag gctacatgaa aatcgttgga
    11821 ccaaccttat tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca
    11881 gaatttccag gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt
    11941 gtgaccattc actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt
    12001 gtgccacgac tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag
    12061 tacaggttgt atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca
    12121 tatgatatct ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg
    12181 tatttgtaat gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt
    12241 tattaacttg gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct
    12301 tagctccaag gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa
    12361 ttgtttttgc tgaaatgtta tttaatctga ttttctttat tccattaggt gtttgtttcc
    12421 ctttgataaa aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt
    12481 tattgtttga gtgcttacag tatattttag caataggtat aacagatata acggatttga
    12541 ctttaaatac gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt
    12601 tcaaatcaca gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt
    12661 atcttgtttt actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata
    12721 taatgactaa acgcgtctta atcagcgtct cagacaaagc ggacattgtt gaatttgccc
    12781 aagaactcaa aaaacttggt tgggagatta tctcgacagg tggaactaag gttgcccttg
    12841 ataatgctgg ggtggatacc attgctatcg atgatgtgac tggtttccca gaaatgatgg
    12901 acggtcgtgt gaagaccctc cacccaaata tccacggagg gcttctcgct cgtcgtgact
    12961 tggatagcca cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg
    13021 tcaaccttta cccatttaag gaaactatcc ttaaaccaga tgtgacttat gatgatgcag
    13081 ttgaaaatat cgatattggt gggccatcta tgcttcgttc agcagcgaaa aatcatgcca
    13141 gtgttacagt tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa
    13201 acggcgaaac ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag
    13261 cggcttatga cgccttgatt gcagaatact tcacagctca agtgggtgaa aacaagcctg
    13321 aaaaactcac tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac
    13381 aagacgcgga cttttaccag aaagctttgc ctacagacta ctccattgct tcagccaaac
    13441 agctcaacgg gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta
    13501 tcatccgtga cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg
    13561 gaattggtca agctgatgac atcgagactg cttgggacta cgcttatgag tctgacccag
    13621 tgtctatctt tggtgggatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga
    13681 agatgcacgg cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag
    13741 ccattttgat caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag
    13801 aggctagcga agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc
    13861 aagacgtggt caaggaaagc ccagctgact ggcaagtggt gactaaacgt cagccaactg
    13921 agacagaagc gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg
    13981 gtattatcgt gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg
    14041 tggcttctgt tcgccttgcc attgaccaag ccaaagatcg tctggacggg gcggtccttg
    14101 cttcagatgc cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta
    14161 aggccatcat ccagcctggt ggctctgtcc gtgaccaaga atccatcgaa gcagcggata
    14221 aatacggctt gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa
    14281 gggaagaaaa cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa
    14341 cgaacttttt tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg
    14401 cttgttgtcg gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa
    14461 gacgtggaaa aagtctttgt agctcctggg aatgatggga tgactctgga tggtttggaa
    14521 ttggtaaata tctctatttc cgaacattct aaattgattg acttcgcaaa gaccaatgat
    14581 gttgcttgga cctttatcgg tccagatgat gcccttgctg ctggtatcgt ggatgatttt
    14641 aaccaagctg ggcttaaggc ctttggtccg actagggctg cagcggagct ggagtggtcc
    14701 aaggattttg ccaaggaaat catggtcaaa tatggtgttt cgacagcagc ctatggcaca
    14761 ttttcagatt tcgaggaagc aaaggcctat atcgaaaagc atggtgcgcc tatcgtagtc
    14821 aaggcggatg gcttggcgct tgggaagggt gtcgtcgttt ctgagacggt tgagcaagcg
    14881 gtcgaagccg ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg
    14941 gttattgagg aattctttga aggagaggaa ttttcactct ttgcctttgt caatggtgat
    15001 aagttctaca tcatgccaac ggctcaggac cacaaacgtg cctatgatgg cgacaaaggg
    15061 cctaacacgg gtggtatggg tgcctatgcg ccagttccac acttaccaca gagtgtagtt
    15121 gatacagcgg ttgacaccat tgtcaagcca gttctagaag ggatgattaa agaaggtcgc
    15181 ccttatctgg gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatt
    15241 gagttcaacg ctcggttcgg agatccagaa actcagatta tcttgcctcg cttaacctct
    15301 gactttgctc aaaatatcac agatatcctg gatagcaagg agccaaatat cacgtggacg
    15361 gacaagggtg tgactctggg tgtggttgtc gcatccaagg gctacccgct aaattattca
    15421 aagggtgttg agttgccagt caaaaccgat ggcgacatca ttacctacta tgcaggggct
    15481 aagtttgcgg aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt
    15541 accacagcag ataccgtcaa agaagcccaa gccagcatct accaagaact atcccaacaa
    15601 aaaatagaag gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat
    15661 aagaataacg cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa
    15721 cagtgaatgt tctggtcagg gggaaacttg gagaccgtag gctcaaagtt taggaatgaa
    15781 accgaaggtt tgcttccgcc tccatcacct aagaccatta tcaaaaagaa aaataaaaat
    15841 tcacaaaata tgttaatgat cgtatggttt gcgagcgtta gcgagctaat atagaacaat
    15901 caccgccgtt gtgaaagaac gattggatga taatccaatc gttcagggaa attggaagac
    15961 cttgggtttc caatttaggc atgagacacc tttggtggct gctgccgtcc ctcacaagct
    16021 aaggtgattg ttgaaaaaga ggaaaaagga gaagaaatga aaccagtaat ttccatcatc
    16081 atgggctcaa aatccgactg ggcaaccatg caaaaaacag cagaagtcct agaccgcttc
    16141 ggtgtagcct acgaaaagaa agttgtttcc gcacaccgca caccagacct catgttcaaa
    16201 catgcagaag aagcccgtag tcgtggcatc aagatcatca tcgcaggtgc tggtggcgca
    16261 gcgcatttgc caggcatggt agctgccaaa acaacccttc cagtcattgg tgtgccagtc
    16321 aagtctcgtg ctcttagtgg agtggattca ctctattcta tcgttcagat gccgggtggg
    16381 gtgcctgttg cgaccatggc tatcggtgaa gctggagcga ctaacgcagc tctctttgcc
    16441 ctccgtctcc tctctgtaga agataagtcc attgcggatg cacttgccaa ctttgctgaa
    16501 gaacaaggaa aaatcgcaga ggagtcgtca aatgagctca tctaaaacaa tcggaattat
    16561 cggtggcggt caactgggtc agatgatggc catttctgct atctacatgg gccacaaggt
    16621 tatcgcgctg gatcctgcgg cggattgccc ggcctctcgc gtggcggaaa tcattgtggc
    16681 accttataac gatgtggacg ccctccgtca gttggcagac cgttgcgatg tcctcactta
    16741 tgagtttgaa aatgtcgacg ctgacggttt ggatgccgtt atcaaggatg gacaactccc
    16801 tcaaggaaca gatctgctcc gcatttcgca aaatcgtatt tttgaaaagg actttttgtc
    16861 aaacaaggct caagtcactg tggcacccta caaggtcgtg acttctagcc tagacttggc
    16921 agatatcgac ttgtcgaaaa actatgtcct caagactgcg actggtggct acgatggtca
    16981 tggacaaaag gttattcgtt cagaagcaga cttggaagca gcctatgcgc tagcagactc
    17041 agcagactgc gtcttggaag aatttgtcaa ctttgacctt gagatttctg tcatcgtgtc
    17101 aggaaatggc aaggaggtga cgtttttccc agttcaggaa aatatccacc gcaacaatat
    17161 cctgtctaag accatcgtac cagcccgcat ttctgaaagt ctagcagaca aggctaaagc
    17221 tatggcagtg cgaatcgcag aacaactcaa cttgtctgga actctctgtg tggaaatgtt
    17281 tgcgactgat gatgacgtta ttgtcaatga aatcgcccca cgaccacata actctgggca
    17341 ctattctatt gaagcctgtg atttctctca gtttgacacc catattctgg gtgttctcgg
    17401 agcaccatta ccagtcatca aactccatgc gccagccgtt atgcttaatg tcctcggtca
    17461 gcatgtcgag gctgctgagc aatatgtgac agaaaattca agcgcccacc tccacatgta
    17521 tggtaaaata gaagcgaagc acaaccgcaa gatgggacat gtgaccttgt ttagtgatgt
    17581 accggatagt gtggatgagc ttggggaagg gattgatttt taggacaagt ctatgataca
    17641 aattatcgtt aatacattta ttgaaaagga taagactgga gcagttgttg aagtgttgta
    17701 tgccagtgct gaccaagata aggtacaagc taaatatgaa gaactagctg cacaataccc
    17761 cgaaaattat ttagctatct atgatgtacc gctggatacg gatttgaata cactagatca
    17821 ttacccgtct gtgtttattg aaaaagagga gtttgagtag aaatcttggt ttacctagat
    17881 agcttattcc caacagctta agaagaaagg aaaaattaac acatgatcaa ccgttactct
    17941 cgccctgaga tggcgaatat ttggagtgaa gaaaataaat accgtgcttg gcttgaggtg
    18001 gaaatcctct ctgacgaggc atgggctgag ttgggggaaa tccctaagga agatgtggct
    18061 ttgattcgca agaaggcgga ctttgacatc gaccgtattt tggaaattga gcaggagacg
    18121 cgccacgatg tggtggcttt cacgcgtgcg gtttctgaga ctcttggtga agagcgcaag
    18181 tgggttcact atgggttaac ttctaccgac gtggtggata ctgcttatgg ttacctctac
    18241 aagcaggcca acgacatcat ccgtcgtgac cttgaaaact tcactaatat catcgctgac
    18301 aaggccaagg agcacaagtt caccatcatg atggggcgta ctcatggtgt gcacgctgag
    18361 ccgacaacct ttggtcttaa attggcaact tggtacagcg aaatgaaacg caatatcgag
    18421 cgtttcgagc atgcagctgc tggtgtagaa gctggtaaga tttctggtgc ggttgggaac
    18481 tttgccaata tcccaccatt tgtagaggag tatgtctgcg ataaacttgg catccgtgcc
    18541 caagaaatct ctacacaagt ccttcctcgt gaccttcacg ctgagtactt tgcggttctt
    18601 gccagcattg cgacttcaat cgaacgtatg gcgactgaga ttcgtggtct acaaaaatct
    18661 gagcaacgcg aagtagaaga gttctttgct aaagggcaaa aagggtcttc agcaatgcct
    18721 cacaaacgca acccaatcgg ttctgaaaat atgactggtc tggcgcgtgt cattcgtggt
    18781 cacatgatta cggcttatga aaacgtcgct ctctggcacg aacgcgatat ttctcactca
    18841 tcagctgagc gtatcatcac accagatacg accattttga ttgactacat gctcaaccgt
    18901 tttggaaata tcgtcaagaa cttgacagtc ttcccagaaa atatgatccg aaacatgaac
    18961 tcgacttttg gtcttatctt tagccaacgg gctatgttga cattgattga aaaaggcatg
    19021 acccgtgagc aagcctatga cttggtgcaa ccaaaaacag cctactcttg ggacaaccaa
    19081 gtagacttta aaccacttct tgaggcagat tcagaagtaa catcacgtct cactcaagaa
    19141 gaaatcgatg aaatcttcaa cccagtttat tacaccaaac gagtggatga tatctttgaa
    19201 cgtcttggac taggtgatta attaaaaaat aaacagcgag cttcaatctc gctgtttatt
    19261 ttttatcgaa aagacttagt cttcttttct tttagtgagt ccataggctg ctagtgtggc
    19321 catgagtcct gcgactacta gtcctgcaga atcgtgagtt cctgtttcag gaagtttttt
    19381 ctctattacc acaggagctg gatcttgagg aagagctttg ctttcctcag caggagcagt
    19441 tgatggagct ggttggcttg ggatttctag ttttggtttt tcttcagcaa tagcggcttg
    19501 tccgttttca tcgcctacat gtgttaccat agttccgact tcgactattt gagtaacggc
    19561 ttcctgtgct acgacactat ttacaagtgt tttcacttcc ttaccatcgg cagaagtgct
    19621 cacagagtag aagttgctac gatgtccatt gacgccctta gtaatgactt gtgtttttcc
    19681 tttgagtaag agtggatttt cacgagtcac tgtggtaaat ggaatttctt ctttttggat
    19741 atccagtcta ggttttacct cagtagttgg tgcaagacca ctttcatcac ccttgtgagt
    19801 tacaggagcg ccaacttcaa ccacttggtt tataacttct ttggttacct ggctatcaag
    19861 gactgtttct gttgtttttc cattttcagt gagtacagag atgtaatgag ttcgctcacc
    19921 tttgactcct gctgtgataa tattttcctg accagctggg aggttaggat tttctttctt
    19981 gataacttca aatggaattt cttcagttct tgtgatgagt tctggtctgg tttcaacatt
    20041 ggcagccact tcattttcat ccaggcttcc tgaatgagtt gcagctggtt tgaggccttg
    20101 aagagcggct tttaggttgg ctacaagtgt gtcaagctca gcttgtttat tacggttgag
    20161 gttgtaattt agagctgttt tagctgcgtc aagggcctca agactttctt tactatatcc
    20221 ttctaagttt gtaggaattt tagctaattc ttcgcggaga gcattataat tagcacggaa
    20281 gtagtctttg ttgtggtctg caaaggcagt catgagttca aagatttcct cttccttgta
    20341 ttcagcgctt ggtctatctg cccagattga aagcatactt ccgactgttg gaagatctac
    20401 ttcaggatat ttggtagaag ctagttgatt gaatggtgtt tttccagtat tctcaatagc
    20461 tttcttgagg aaaccaccac catcttctgg tttttgacca agaatgtagt accagtcacc
    20521 gttggtattc aagaatttat agcctttgct tgctaggtat tgaggtgatg cgaggttata
    20581 tccccaccag cctttagacc agtaagaaat caagacatct ttgtcaaact gaacatcgtc
    20641 cttgtcttca tagtagaagc catcgttgaa ggccattggt tgaagccctc tttctttggc
    20701 catagctgcg agggtgttgg catattcggc aaatttgcca tagagttgat accacttgag
    20761 gtagtaccag ccttgggcac tagtcgcatc gttggcgtat tcgtcagtac caaagttgaa
    20821 aatctttgtt ttacctgcaa agaagtccat gtatttaccg atgagggctt ttacaaagtt
    20881 catcgcttct tcgtttttca agtccatagt tgtttttgaa actttatcaa agtgggcttg
    20941 aggattttta atacctaatt tttccatggc aaccagcata gcatccatgt gacctggact
    21001 gttaatagct gggatgagac cgatgtcctt agatttagcg tattcaatta gctctgttac
    21061 ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag taagctttag ttccttcgat
    21121 aatagctttt ttaacgtcat cactagcata ggtttttccg ttggcagtaa tggtcatatc
    21181 atcgagtaga aagcgaagtc cgtcatttcc tagaaggaga tggacatcag aatatccgag
    21241 ctcactggcc ttgtctacga tgcgtttgag ctggttcaga gtaaagtatt tgcgtccagc
    21301 atcgattgag attaccttgt ttttggcaag tttttcaacc tcacgtttag cttcttcttc
    21361 tttttgagct tcaggcgtga gggtcaagtt gttgacagtt tcttgaagtt tagcaatggc
    21421 ttgatcaatc gtatcttgtt gggcacggct aaggttgcta tcgagagagc gaatagcttt
    21481 ttcagcttct tttacggccg cgacgctttc tgcagtataa cggttcaggt cttttggtac
    21541 ctcgttaagt gtttgctctg cagattcata atcagctgcg aagtattcag cgttggcatt
    21601 tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa taacgtgcag atggagtgtc
    21661 agcccaagca gctaccatac taccgatgat tgggatatca gctccttctg tttttggtac
    21721 agaagtgatt ggtgtgtttt taataccatt gagcccctga tcgagattgt accagccttg
    21781 gccatcagcg tttcgtccaa gaacgtagta ccaagcatca ttggtattaa ggatttggtg
    21841 acctttttca gctagtagtt tagaagaagc gacatcgtag cctccccaac caccagtcca
    21901 catagaaacg atgatgtctt tgtcaaaact accaaagctt gtgtcgctat tgtagtagat
    21961 accgtcgtta aaggccattg gtttgagacc gtgcgatttt acaatacgag cgaggtcatt
    22021 ggcgtaggca ataaattttt catagccttt tacagggtag ccttcgtttg gatagtattt
    22081 atcagcttga agcacactcc aacctttagc atctgtcgca tcattggcat attcatcaag
    22141 tccgatgttg aagatttcag tctttttcgc gaaataagca gcatacttgt cgataagggc
    22201 ttttgtaaaa gcgacagctt gttcgttgtc aagattgaca gtacgggctg attccttccc
    22261 aaaatagcta aagttagggt tttggattcc caattctttc atggcattga gaatcgcatc
    22321 catgtgacca ggactattta ctgtcggaat gagaccgata cctttatctt tggcatagtt
    22381 aatcagatct gtcatttgac tttctgttaa gtgattgccg tttggatcgt tgtaataatc
    22441 atttgtacct ttttcaatgg cgcgtttgac atcgtcactg gcataggtct tgccgttagc
    22501 tgtgatgctc atatcgtcca acatgaaacg gagtccatca tttccgacta ataggtgtaa
    22561 atcagtgtag ccataatgtt tcgctttatc gatgatttcc ttgagctgtt ctggtgagaa
    22621 atatttacgt ccagcatcaa tagaaacaat tttctttttc gctagttttt catttacagt
    22681 tgcagcacgt tcctttcctg cctctgtttc cggtttgtca tcctctgctt tcgcttcatc
    22741 ttttttagct ggtttatcct tgtcagtctt gtctgtattt gactctttag aatcaacctc
    22801 tttcgcttct tcctttttag ggctagcttc ttctgccttt ttattagcag tttctttttc
    22861 agcagaagtt ggagttacca cttctgcttt atcactagga gttgaactaa cttcctcttg
    22921 tggtttttct tctgtttttg gaagactagc taccttatca gtagctggag tttctgtttc
    22981 tacagttttt ggagcttctg gttgaagctc tgctttaggt gtttcctcag tccgattttc
    23041 ggatgattga ggggaatcag aaaccgtatg gatggtcggt tggttttctg tagtagtagg
    23101 agtaactcca tcggctgcaa cagtctgtgc ttggaaggca aatccaatta gaacagaagc
    23161 tgctcctaca gcgtatttac gaatagaaaa acgctgttgt ttttcatgtt tcattgcaaa
    23221 acctcctgat tgcattgtta tattgatagc gattatataa atcaacgcct ttattttatt
    23281 tcttatatta acgagagtca agaggagatg acaaaaaact ataataagta taaaaaaata
    23341 taaaatttaa acttaagatt tcagattggt cggaaaaaat acgtatatat atctagtata
    23401 atttttggtt ctatttctat aaaatattcc acaaattata gaattttcca aaaataggta
    23461 agcgctacct ttttggtgtg gtataataag catagaaaaa gcccaagcga ttagctcagg
    23521 ctttcttctt agtgatcacg gtcacatgag ataaatttaa tcttgtagta atcagatcgt
    23581 ttgtaagttt cactgtattc taaaacttgg ccagttgatt cgagtttggt gattttagtt
    23641 tgtaggacag tagggaattg ttcatcgact ccgaggactg aagccgcatg ttctggagtt
    23701 ggaaagacta tttcgttgat ttcttcaaag tgttcatcat tcatgtgaat gtggtagtct
    23761 aacttgaaac gattatagat agaactatag tattcaaggt ttggataatt tgcgttgata
    23821 tattgttctg ggatgtagga tgtatggtag atataaacga caccgtttga ttcgcggata
    23881 cgttcaatct tgtagtagaa ttgatcgccg cgtagaccca atttttccaa gtaaacaagc
    23941 ttgtttccgc gttcaattga aagaacagtt accttatcat ctttagcatt gaagagttca
    24001 atatctgaaa actctacaag cttgtgtttg cgtgcacgtg aaacgaaggt tccttttcct
    24061 tgttggcgga caatatagcc atctttggca aggtcgttta aggcgcgaac aactgtgata
    24121 gagctgacat cgtacattga aatgagttct gcttcagtgt aaaatttatc tccactgcta
    24181 aactgcccag agatgatttt attttttaat tcgtctttta tgtattgata tttgggaatt
    24241 gccatatttt tacctctatt ttccttctcc ataggaaatt ttaccataaa agcgaaaaaa
    24301 atagtagtct tttgaataaa aaattagaat aatagtttga aaaattaagc gatataggga
    24361 ttgctcatat tttatcaatg tattctatac aaaattattt taggtcccat attccgaatt
    24421 taaagagcgt ttgaaatggg tctaaaaatg tttgaaacta attgaatgtg tgtataatag
    24481 taaaaatgca gtaaatatcc cgacaatttc tattgacaat tcaaacagat tggtttataa
    24541 ttaatataac aacaaatgaa agcgcaaact ttcgcggtcg gaaggtagtt ttatgacacg
    24601 atttgagata cgagatgatt tctatctcga tggaaaatca tttaagattt tatctggtgc
    24661 cattcattat tttagggttc ctccagagga ttggtatcat tcgctctata acttgaaggc
    24721 tcttggtttt aatacggtag agacttatgt tgcttggaat ttacacgagc ctcgtgaagg
    24781 tgagtttcat tttgaaggtg atctggattt agagaaattt ctccaaatag cgcaggattt
    24841 gggtctctac gcaattgtgc gtccgtctcc atttatctgt gcggaatggg aattcggtgg
    24901 cttaccagct tggctcttga ccaagaacat gcgaattcgc tcatccgacc cagtatatat
    24961 cgaggcagtt ggtcgctact atgatcagtt attgccaaga ctggtgcctc gtttgttgga
    25021 caatggtggc aatattctca tgatgcaggt tgaaaatgag tatggttctt acggagaaga
    25081 taaggcttac ctgagagcga ttcgacagct aatggaagag tgtggcgtaa cctgtcccct
    25141 ctttacatca gatggtccat ggcgagctac tctgaaagct ggaaccttaa ttgaagagga
    25201 cctctttgta acaggaaact ttggttctaa ggcaccttac aacttttcgc agatgcagga
    25261 attctttgat gaacatggta agaaatggcc actcatgtgt atggagttct gggatggttg
    25321 gttcaatcgc tggaaagaac cgattatcac acgggatcct aaggaattgg cagatgcagt
    25381 tcgagaggtt ttggaacaag gctctatcaa tctttacatg ttccacggtg gtacaaactt
    25441 tggtttcatg aatggttgct cagctcgagg aactttggac ctgccacaag ttacgtctta
    25501 tgattacgat gcccttctgg atgaagaagg aaatccaact gctaaatatc ttgcagtcaa
    25561 gaagatgatg gcaacacatt tttcagagta tccgcagttg gaaccactct acaaagagag
    25621 tatggagttg gatgctattc cactagttga aaaagtttct ttgtttgaaa ccttagatag
    25681 cttgtcaagt cctgtagaaa gtctctatcc tcaaaagatg gaggagctgg gacaaagtta
    25741 tggctaccta ctttatcgaa cagaaacaaa ctgggatgca gaagaagaaa gacttcgtat
    25801 cattgatggt cgagataggg cccagctgta tgtcgatggt cagtgggtta aaactcaata
    25861 tcagacagag attggggaag atatttttta tcaaggtaaa aagaaagggc tatctaggtt
    25921 agatatcttg atagaaaata tggggcgtgt caactatggg cataagttct tagcggatac
    25981 gcaacgtaag ggaattcgga caggggtctg taaggatctg catttcttac taaactggaa
    26041 acactatcca ctcccactag acaatcctga gaaaattgat ttttcaaaag gatggactca
    26101 aggacaacca gccttttacg cttatgactt tacagtcgaa gagccaaaag atacttacct
    26161 agacttgtct gagtttggta agggagttgc ctttgtcaat gggcagaatc taggacgttt
    26221 ttggaacgtt ggcccaactc tctcacttta tatccctcat agctatctca aggaaggtgc
    26281 caaccgtatc attatctttg aaacagaagg tcaatataaa gaagagattc atttaactcg
    26341 taaacctaca ctaaaacata taaaggggga aaacttatga caattgtagg atgccgtatt
    26401 gatggacgtt tgatccacgg acaagtagcc aatctttggg ctggaaaact aaatgtttca
    26461 cgcattatgg ttgtagacga cgaagttgtc aacaacgata ttgaaaagag tggtttgaaa
    26521 cttgcgacac caccaggtgt gaaattgagt attttgccag ttgagaaagc tgcagccaat
    26581 attcttgctg gtaaatacga tagccaacgt ctctttatcg tggctcgtaa accagaccgc
    26641 ttccttggtt tggtagaagc aggtgtacca cttgaaaccc ttaatgttgg gaatatgtct
    26701 caaacaccag aaactcgttc tattacacgt tctatcaacg tagtagacaa ggatgtggaa
    26761 gacttccaca aactggcaga aaaaggtgtt aaacttactg ctcagatggt tccaaatgat
    26821 ccaatttcag actttttgag cttattaaaa taggaaaaaa atttttagga ggtcattgtt
    26881 atgatacaat ggtggcaaat tttacttctc actttgtact cagcttatca aatctgtgat
    26941 gagttgacga tcgtttcatc tgcaggttcc cctgtatttg ctggtttcat tactggttta
    27001 atcatgggag atgtgactac tggtttactt atcggtggta acttgcaact gttcgttctt
    27061 ggggttggta ccttcggtgg tgcttctcgt atcgacgcaa cttctggtgc ggttcttgcg
    27121 acagccttct ctgtttcaca aggaattgat gcaccgcttg caattactac aatcgctgta
    27181 ccagtagcag ctctcttgac ttacttcgac gttcttggtc gtatgactac taccttcttc
    27241 gctcaccgtg tggatgctgc aatcgaacgc tttgactata aaggtattga acgcaactac
    27301 ttgcttggtg cgattccgtg ggctctatct cgtgcccttc cagtcttctt tgcccttgct
    27361 tttggtggtg cctttgtaca atcagtagta gacttcgttg aagcctacaa atgggttgca
    27421 gatggcttga cacttgcagg acgtatgctt ccaggtcttg gatttgcaat cttgcttcgt
    27481 taccttccag ttaaacgtaa ccttcactac cttgctatgg gatttggttt gacagctatg
    27541 ttgactgttc tttactcata tgtaacaggt cttggtggcg ctgttgctgg tatcgtaggt
    27601 actcttcctg ctgaagttgc tgaaaaaatt ggtttcgtga acaacttcaa aggtttgtct
    27661 atgattggta tttctatcgt aggtattttc cttgcagtgc ttcacttcaa aaatagccaa
    27721 aaagtagctg tagcagcacc ttctacacca tcagaaagtg gggaaatcga agatgacgaa
    27781 ttctaattac aaacttacaa aagaagattt taatcaaatc aacaaacgta gcttgtttac
    27841 tttccaatta ggttggaact acgaacgtat gcaagcttct ggttaccttt acatgatctt
    27901 gcctcagttg cgtaaaatgt atggtgatgg aactcctgaa ttgaaagaaa tgatgaaagt
    27961 tcatactcaa ttcttcaata cttcaccatt cttccatacc attatcgctg gttttgacct
    28021 tgccatggaa gaaaaagatg gtgtaggttc aaaagacgct gttaacggta tcaagacagg
    28081 tttgatggga ccattcgctc ctcttgggga tacaatcttt ggttcacttg tacctgctat
    28141 catggggtca gtcgcagcaa ctatggctat cgctggccaa ccttggggga tcttcctttg
    28201 gattgcagtt gcagtagcgt atgacatctt ccgttggaaa cagttggaat ttgcttacaa
    28261 agaaggggtt aaccttatca acaacatgca aagtaccttg acagctttga ttgacgctgc
    28321 atctgtactt ggtgtcttca tgatgggtgc tcttgtagca acagtgatta actttgaaat
    28381 ttcttacaag ttgccaatcg gtgaaaagat gattgatttc caagacatct tgaaccaaat
    28441 cttcccacgt ttgcttccag caatctttac tgcctttatc ttctggttgc ttggtaaaaa
    28501 aggtatgaac tctactaaag ctatcggtat tattatcgta cttgctttgg ctctttctgc
    28561 ccttggtcac tttgcacttg gaatgtaatt ccttatgact aaatcattaa ttttggtgag
    28621 ccatggtcgc ttctgtgagg agcttagagg tagcacagaa atgattatgg gcccacaaga
    28681 caacatttac acagtagctc ttcttccaga agatggccca gaagaattta ctgctaaatt
    28741 tgaagctgtt attgaaggat tggatgattt cctagtcttt gcggatcttc tcggtgggac
    28801 accttgtaat gtggtgagtc gcttgatcat ggaaggtcgt gatattgacc tttacgcagg
    28861 gatgaatctt ccaatggtga ttgaatttat caatgcgagc cttacaggcg cagatgcgga
    28921 ctacaagagc cgtgctgcag aaagcattgt gaaagttaat gacctgttag cgggcttcga
    28981 tgatgacgaa gatgaataat actcttcgaa aatctcttca aactacgtca acgtc
//
LOCUS       DAVBCR010000023        25613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCR010000023 DAVBCR010000000
VERSION     DAVBCR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.023"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..338
                     /locus_tag="VQ508_000899"
     CDS             <1..338
                     /locus_tag="VQ508_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2489919.1"
                     /translation="CLSETADKNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKP
                     IPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHVTKNMKDEAINV
                     LDKVMKKIF"
     gene            complement(830..1240)
                     /locus_tag="VQ508_000900"
     CDS             complement(830..1240)
                     /locus_tag="VQ508_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489920.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1258..1635)
                     /locus_tag="VQ508_000901"
     CDS             complement(1258..1635)
                     /locus_tag="VQ508_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489921.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1577..2005)
                     /locus_tag="VQ508_000902"
     CDS             complement(1577..2005)
                     /locus_tag="VQ508_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2489922.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2049..2654)
                     /locus_tag="VQ508_000903"
     CDS             complement(2049..2654)
                     /locus_tag="VQ508_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489923.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2744..2917)
                     /locus_tag="VQ508_000904"
     CDS             complement(2744..2917)
                     /locus_tag="VQ508_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489924.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3516..4412)
                     /locus_tag="VQ508_000905"
     CDS             complement(3516..4412)
                     /locus_tag="VQ508_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2489925.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4424..5233)
                     /locus_tag="VQ508_000906"
     CDS             complement(4424..5233)
                     /locus_tag="VQ508_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2489926.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5308..5523)
                     /locus_tag="VQ508_000907"
     CDS             complement(5308..5523)
                     /locus_tag="VQ508_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489927.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5525..5725)
                     /locus_tag="VQ508_000908"
     CDS             complement(5525..5725)
                     /locus_tag="VQ508_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489928.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5722..5913)
                     /locus_tag="VQ508_000909"
                     /pseudo
     CDS             complement(5722..5913)
                     /locus_tag="VQ508_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5902..6192)
                     /locus_tag="VQ508_000910"
                     /pseudo
     CDS             complement(5902..6192)
                     /locus_tag="VQ508_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6362..6514)
                     /locus_tag="VQ508_000911"
     CDS             complement(6362..6514)
                     /locus_tag="VQ508_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489929.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6525..6791)
                     /locus_tag="VQ508_000912"
     CDS             complement(6525..6791)
                     /locus_tag="VQ508_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489930.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7002..7184)
                     /locus_tag="VQ508_000913"
     CDS             complement(7002..7184)
                     /locus_tag="VQ508_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2489931.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7246..7749
                     /locus_tag="VQ508_000914"
     CDS             7246..7749
                     /locus_tag="VQ508_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2489932.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7823..8986
                     /locus_tag="VQ508_000915"
     CDS             7823..8986
                     /locus_tag="VQ508_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2489933.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQ508_000916"
     CDS             complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQ508_000916"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2489934.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10608..11054
                     /locus_tag="VQ508_000917"
     CDS             10608..11054
                     /locus_tag="VQ508_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2489935.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11051..12166)
                     /locus_tag="VQ508_000918"
     CDS             complement(11051..12166)
                     /locus_tag="VQ508_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2489936.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12175..12504)
                     /locus_tag="VQ508_000919"
     CDS             complement(12175..12504)
                     /locus_tag="VQ508_000919"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2489937.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12458..12820)
                     /locus_tag="VQ508_000920"
     CDS             complement(12458..12820)
                     /locus_tag="VQ508_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2489938.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQ508_000921"
     CDS             complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQ508_000921"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2489939.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQ508_000922"
     CDS             complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQ508_000922"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2489940.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15438..16580)
                     /locus_tag="VQ508_000923"
     CDS             complement(15438..16580)
                     /locus_tag="VQ508_000923"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2489941.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQ508_000924"
     CDS             complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQ508_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2489942.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            complement(18980..20404)
                     /locus_tag="VQ508_000925"
     CDS             complement(18980..20404)
                     /locus_tag="VQ508_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2489943.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(20948..23227)
                     /gene="pulA"
                     /locus_tag="VQ508_000926"
     CDS             complement(20948..23227)
                     /gene="pulA"
                     /locus_tag="VQ508_000926"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2489944.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(23339..25297)
                     /gene="ligA"
                     /locus_tag="VQ508_000927"
     CDS             complement(23339..25297)
                     /gene="ligA"
                     /locus_tag="VQ508_000927"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2489945.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(25390..>25613)
                     /locus_tag="VQ508_000928"
     CDS             complement(25390..>25613)
                     /locus_tag="VQ508_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2489946.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         7598 a         5434 c         4402 g         8179 t
ORIGIN      
        1 attgtctaag cgaaacagcc gataagaatt ggcacaaaag cgaccgtatt tttgttacca
       61 atacaggaaa aacagttcat agttctatct tgagcaagtc tctccagcga gcaaacgaac
      121 gccttaaaaa accaattccc aaacatctgt cccctcacat cttcatacac atcactatta
      181 gcatcttatc agaaaataaa attcctttaa aaacaatcac ggacagggtt ggtcatcccg
      241 actctgaagt cactaattcc atctacaccc acgtcacaaa gaacatgaaa gatgaagcaa
      301 tcaatgtact ggataaagtt atgaaaaaga ttttttaaaa agttttgtcc cttttttgcc
      361 ctctaaatac aaaaatagcc cttcggataa aatccgaggg gctagaaacg ttgttaaatc
      421 aacggccgaa cttttgaatt tcatggttcg ggataaaata gttcactgaa ctattttatt
      481 ttttaaggtt atcataatat caaatagttc aattaaatac gctaaattac taatatactt
      541 tttacctttt tcattctaaa atgtaaagta caaacaatta caatatacta gagggggagt
      601 aaaaaaggta ataaaattga cgagttcagc aggcaaggga aatagcactt ttcatggtgt
      661 tattttttta attcagcaat ggagtagccg cttaattgat agaaacaaaa agcacctagt
      721 ttcctagatg ctagcacaat agcccggatt cgaaccggaa tctcctcaat taaggtgcaa
      781 tccctatata ctactcattg tgctttggtt cattatatca tacaatcaac tagctatcta
      841 ccaactcatg atacgctccc atattcggta atggaataat actaaaatac aaatctgagt
      901 aagtttcaaa ccatttattc agcttgttat aggctggcga ctgactaaca aataatatcc
      961 gttgacaagc tccgataaca ttcatgtgat tatacacata gacctcttta atggccgtta
     1021 aaatatcgtc ttcacttgtc tgtaccctaa tatctgttat ctgcttaatc gtagcgtatt
     1081 ttttataagc tagcatatct ttatttttag cagcatcaaa cattttacac tcaagaacac
     1141 tatacttagg tttttcttta tctctcatga aataccactt gagccataaa attttttctc
     1201 tgtgaattac tgaaatacgt tcgatttttt tctttggcat gattacctcc taaattatta
     1261 atctaatagc tttaaccact cactttttga gattgttgta taaggttcat ctagcgcgtg
     1321 atataaaggt tctaaacctt tgttaaatcg gatatagtcc attttatcct catgctttgg
     1381 aaaatataag attcctgttt catattcatt tagaccacgg ttacaccaat catcaacgat
     1441 aacctgcact tcccattcac gccacttttt aaggttttct aaaagttcaa tatcactaaa
     1501 taactcacca tcaacttgga aaaattctcc cagatcatcg ttaccctcga cgatgataac
     1561 ttcaggcgta tttctactac ttaataattc cttgagttct tgtaactctt tgatctcctt
     1621 tagatacttc ctcaatttct aacctcaatt cttcaatttg tcttactact ccaaaaattt
     1681 catgagtttt atatgattgt tcaagtatag tttttgctgc ttgagttctt ataaaagggt
     1741 taattttggc atccatcata atatcattga gtacagaaac agcattggat gaggctagat
     1801 atagcatctg tgtagtttta tccatcatct catcttgttt tagtctcaat gtctttttaa
     1861 cagctgcaac ttttagatac ttatgccctg ttgcccttga cacccctgct ttttggcacg
     1921 ctttatctat cgttggctca gtaagcatgg catctatgaa ttttatttgt ttagatgtaa
     1981 ggccatcatt ttcatttcct gccatctatt acctcctcgt tatcaaaata aaggggttgc
     2041 tagcccctct atttgtttaa atactcatcc aaacgacggt caagctcagg ggcatctgaa
     2101 tttactacca tttcagttgt tacagcttgg attttatcgc cgttgataag caaactgtaa
     2161 tgacaatatg aaagttgccg cttaaagaat aagattagac tttctagcgc ctgctctgca
     2221 ttgaataatt ccaagttgat agcaatttct ggatattgtt cagcaattga ccaaagtttt
     2281 ttaatagcaa gaggtttgtt cttgtatttt tcaaaataac ctagcaattc atcctttgtc
     2341 atcttcatac tagctaacaa ggtcagctct gccacgtcat cagctgttac gctgagttct
     2401 ttttctttta atgcctctag ctctagatca tgctgttttt caatttctgc atgaatttta
     2461 ttataggtgt ccctagaata agtgtccccc tctaacttat aagcctctag ctggtcttta
     2521 gcgtattttt cgcctatcat accattttgt aactgtttat gtaattcctc ttgtttagca
     2581 atgtagccag tcatgtaccc atgctttttc ttgccaaaag ccttaatatc atccttgatt
     2641 gttgtaaatg tcatgttatc ctccttacga aaaaataaaa gaggtatgac aaaagagcgt
     2701 taaactcaat tatcatacct ctggtttttc cagtcagtat ttattaacat tgttctccag
     2761 tggtcacttc cttataaact aactgcccat cttgagcttt tagcttaatg ctaccatgat
     2821 ctggaatttc aatgtacttt attataccat tttttgagta taaaacaaag cctttctcca
     2881 tcatttctaa taatttatta tccttgtttt cagtcatgaa tttctccttt atttcaagtg
     2941 tcccaacggg tacggttgta agctgagaca ttttagggct atctttgagc gacaaaaatg
     3001 tcgaaaagtc ttcagttcct gtataaccgt tattagtttt gtttgctact catttttgag
     3061 tcacaaatct gttaagcaaa aatgagtaat agctcgtaag ataagtaaat ctaatacttg
     3121 tattcctaaa gcgttgatat aactgatatt ttcaactatc caacgctctt tactaccctt
     3181 tttagtgtgc tttagctcat ttttgagctt ttttgagctt ttttgagttc cattttggga
     3241 atgcaaagca ggcgcgcacc gtgcatgctt ttttcttgct catagcctca ccctcagaag
     3301 tttgccgacc gagagcgtgg ggctttttat ttttgtataa ttaaaaacag ctgcacaatt
     3361 tttgtacagc tacgaccgag gcaggggcga cctgccgtac tttacactag ataatatcat
     3421 ctaggtagtc caactgtatt ttatcatatt aaaggctaca ttacaatttc tcaccctgtg
     3481 aagagtgtct atttttgttt aggtttgtaa gtcgattact tcaactgctt taccttattt
     3541 tttaactgat taattgagta tcgcttatct ttgattgtga aagccttaaa agtgttaccc
     3601 tcaagacctt ttagaatacg gctataattc cgctcgttgt atactatctt gagttcatcg
     3661 ctatcaagat ttgtattaaa tatagtattt tctcgattat tcagtacatc aaaaagtaaa
     3721 tcctgctccc aatcgctctt aggtttaata acagcatttt ttgcacctaa atcatcaata
     3781 attaagtaat caacagactt catgagttca gtagcttcaa actctgtaag tgttgcacct
     3841 ttaccataat tccacccctc tttaatttgt ttgatcattt cggttaggct tacaaaaagc
     3901 acactcttag gttctccttt tgtcttatac ccctcattta tacctttggc aatagcaact
     3961 gataaaagtg tttttccaat ccctgtacct cctgtgataa gcgtatttcc cctcatgcca
     4021 tcaagatatt tttgtacctg accttttgca aattctaaaa atcgcttttc ttctgatgtt
     4081 acagcattaa aatcatcaaa agttttagtt ttaaactcat ctgctacata gctcttattg
     4141 ctcatcaaca cattataagt ttgcagatat agtttagcat tcaaattatc agcaatcgca
     4201 tcttcttcat cttgcttttt ctgttcttct tggcattgtt cacaataggg tgggatacag
     4261 cgaacttctt ttattacctc tccgttctca ttccacccca ctactacatg tctttctcct
     4321 ttgatttgtg ttagctgtat ttcatgctta ggacacaatt cgtctagttt aaatgtctca
     4381 atatttccta aactagattg taatgatttc attttctgac ctcctaaaat ggtttttctt
     4441 gtgttggtat ccaatcttca tagctggtag gctctagttg attggtttgc tgttttttag
     4501 cctcacgcgc tgccctgcta tttctaacaa gttccaccgt caataaattg tcctgtttcc
     4561 aacggttaag gattgccttg atgtatgcaa agtttgcttt accctgactg acagcctctt
     4621 ttaacgcctc atggataagc tctgggctaa aatcttctag catatactgc aattcttgaa
     4681 tctgtaacgg tgacaatgct ttacctgtct cagcttcaaa agatttatag agatttacaa
     4741 aatcttgatt gatggtagag ggagtgggtg attgttcttt atctacttct atctctaatt
     4801 ctttctctat ctctgttgga catgagttgg aaaatgtcaa agatggttgg acttcttcca
     4861 attttccttt ttttcgttgt tcacgtttat aactcgccca gtttgtttca ctcataacca
     4921 tagcttttgc ctgtggtagt tctgcgtttt tatcttcgtc aatctgtatt aaaccgcatt
     4981 ttgtaaagta tgccatggtc atatctatat catcttctga tacatctaat tttagggcta
     5041 actcttcctt gagattgtta aagtagccct catagtataa aatacagtca ctttccaagc
     5101 tctctaacat gagcctaata taaataactg tcatagtgta cccaccaggt accgtcttta
     5161 gtctcttgat aaaaatatta tcaaaaaatt tcttatcaat ttttagccaa aaatagactt
     5221 ttgtttttgc catcgtcctc tcccttgctc atttataatt ctctaccttg attagtctta
     5281 atactcgctt gtcattcatt gttagactca ttcaattctt ccccatacgt ctacccctgc
     5341 aagctggata tatcgcccat aatcagggtt taaatcctcg ctaggtgttt ctatcgtctg
     5401 ttggttttct cgctccagtt gggcgctttt tttgcggtct cggtggttta aataaagcag
     5461 taagccaatc aaaatcacgg taacaaaaac cgcctgtgta ttgcttatat ctagttcatt
     5521 catgttatgc cctcgctttg taattcttga tgtatgccac ttgttcagct cgcttcatat
     5581 tcaacaagtc gtctatttcc acactggtta cttttttatt tacaaaatca gaaatcagtt
     5641 gaaaaatgtt tggactttgt agctggattt cagccattgc ctcatcaaat tctgcttgtg
     5701 tcatgttgtc taaatctagt gtcattgcat tgcctcctca aacttctcta taagacaact
     5761 tttatttgct ttctgagttc catttttaga gttaaaaaga atatatttta aggttacagt
     5821 agcctctaaa tactcctttt cagcatgctc tatatacgcc tgttgctctg cttcgttctc
     5881 aaaaaagtgc ttagcttagc gtttaaagaa tgctttgact aggtttagtt tgaattgccg
     5941 tactgcttcg gtattcttta gataagtgat cagcaaagta gcctgttgct cgttcagaat
     6001 ataggatttt ttaggttgtc ctctagtgtc taatttatgg attttaaatc caagtattcc
     6061 caactcttca aagtcaacct tattttctct tattaagcgt gtgatagtgt ggtgttgtac
     6121 gtcagcacat tcagcgatga tctcgcttgt ggtatacggc tctttcttgc cgtccatgta
     6181 gactagttcc attatggttc tacctcctgt ataaatttag ctagcttact ttttaattgt
     6241 ctcctctagc cctttttagc ctctacaacg gttttggcta acgtgttaga ggcttttata
     6301 acggcctgta tagctgtctc tatttcctcc gtggaatagt agctttgttt tctaatatca
     6361 ttcaagactt gtttaaccca tttcttgaaa taaataaaat tacatcttct gtactcttgg
     6421 catctgcttt atctgagaca aatagaatgt caatgcctgg tagataaaac ccaccgtcta
     6481 tttgtttagt tggtttgaaa gtaactattt tcatttcata actcctattt tattgctaaa
     6541 aataaataca catctgacag acgatagaat actttcttag ctccatccat cggggtctga
     6601 tatcgtttaa gagcaagatc ctcccagtta tcaagggttg tagcacttat acctagcaga
     6661 tccattaatt ctttacgaga aattaaatct aacttactag atcgttgttt ttctagctca
     6721 actaaagttt caagagtctg tttaagaagc tgtaaaccct gtaactgtat ttctttagac
     6781 agaatttcca tttatatcct ccattttcct ttttataaat taagttgtct ttttcctcta
     6841 attttgataa aataagaaga gattatttag caaagggggt tgtccctttg ctaaatacct
     6901 aacaaaacca cgcgccttgc ctttgcttga gttttgttat ttttccttag ccgtgtacgt
     6961 tggtttggtc gcctgtacat ggctttttac ttgcgttaaa tttattctac tagccaagtc
     7021 atcacgctct catagatacg cttagaggca tcatagtttc cattctcaac ttttgttagt
     7081 gtttgagatt taatacccaa ctgctccgat actgcttttt tggttagctg taatcttgca
     7141 cgcttgatac gtactttttc agcaagtgat agtgtaatca acattggttt cacctccctt
     7201 ttttctccat ttttggcatt ttgtgttata atctctttag aaaggatgta taagatgaat
     7261 agaattaagc aattacgaaa agaaaataat cttactcaaa gagaacttgc taatgagact
     7321 aaaatccctt atagaaccat acaacgttgg gagaatgggg aaactgatat aaaatccgat
     7381 gctgctcagg tacttgctga ctattttgga gtatcaattc ctcacttact aggttatagc
     7441 gatttgatag cagatacaga tagtgatatt aaagcaatgg catactcaca tctactaact
     7501 tttgtagatg aaaaacaaat caaagaattt gaaaaagagt tcattcgtgc aaacgataat
     7561 agtgatactt ttggtgattt aggaagtggt gaaaaggcgt tagaaaaata ctatcaagca
     7621 ttatgctatt tagttcctcc ttttagggat atattggcta ggtatagctt tttgtcacta
     7681 gatgaaaaaa aatcagtcta taatattatt gttggtttaa gtaacaactt atcaaaagaa
     7741 aataaataac tttaaacatc atgtataaat acctaacaaa accacgcgcc ttgcctgctg
     7801 atggaaagaa aggtacaaat acatgaatat catagagaaa attaaaaaga atggccaaag
     7861 agtttattat gctagtgttt atctaggcgt tgaccaacta acgggcaaaa aagcccgtac
     7921 aactgttaca gcaaccacta aaaagggcgt taaagtaaaa gcgcgtgatg cgatcaatac
     7981 ttttgctgct aatggctata cagttaaaga taagccgaca attacaacat acaatgagct
     8041 tgtaaaagtt tggtgggata gttacaagaa tacagttaag ccaaatactc gccaatccat
     8101 ggatggattg gttagagtgc atttattgcc tgtatttggc gattacaagc tatctaaact
     8161 tactacgcct attcttcaac agcaagtaaa caaatgggct gacaaggcaa ataaaggcga
     8221 aaaaggggca tttgctaact actccttgct ccataacatg aataagcgta ttttgaaata
     8281 tggcgtagct atccaggtaa tacaatacaa cccagctaat gatgtaatcg ttccacgcaa
     8341 acagcagaaa gaaaaggctg ctgtcaaata cttagacaac aaagaattaa aacagtttct
     8401 tgattattta gacactctga atcaatcaaa ttatgagaac ttatttgatg ttgtcctgta
     8461 taagacttta ttggccactg gttgccgtat tagtgaggct ctagctcttg aatggtctga
     8521 tattgaccta gaaagcggtg ttatcagcat caataagaca ctaaaccgct atcaggaaat
     8581 aaattcacct aaatcaagcg ctggttatcg tgatatacca atagacaaag ccacattact
     8641 tttactgaaa caatacaaaa accgtcaaca aattcagtct tggaaattag gccgatctga
     8701 aacagttgta ttctctgtat ttacggagaa atatgcttat gcttgtaact tacgcaaacg
     8761 cctaaataag cattttgatg ctgctggagt aactaacgta tcatttcatg gtttccgcca
     8821 tacacatact actatgatgc tctatgctca ggctagcccg aaagatgttc agtatagatt
     8881 aggccactct aatttaatga tcactgaaaa tacttactgg catactaacc aagagaatgc
     8941 aaaaaaagcc gtctcaaatt atgaaacagc tatcagcaat ttataaaaaa taagggtgac
     9001 ccatttccgg gctaccctct tactatacca aaaattagta ggggtagtaa aaagggtatt
     9061 aaattataaa aagcactaag ggaaagcgcc ccaaagtgct tatttcaaag gctttatagc
     9121 ctataatcac ataaggagat tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     9181 cagctacttc gccaagttgg tcttcgatac gaagcaattg gttgtattta gcgatgcggt
     9241 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcaatatcag
     9301 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     9361 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     9421 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     9481 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     9541 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     9601 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     9661 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     9721 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     9781 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     9841 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     9901 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     9961 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
    10021 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
    10081 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
    10141 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
    10201 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
    10261 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
    10321 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
    10381 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
    10441 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
    10501 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
    10561 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
    10621 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
    10681 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
    10741 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
    10801 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
    10861 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
    10921 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
    10981 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
    11041 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
    11101 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
    11161 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
    11221 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
    11281 cagcaaagct ttgactatca agtcttcctt ctccaacaac aaccaagtca gcatctgaaa
    11341 ctttcttatc aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
    11401 gagcaaaagc acacaaacca ccagcaaggc ctccacctgc tcctgctcct ttaatttcta
    11461 atgttgcagg tgagaatttt tcataaaaat cttggatcgc ctgatctacg actgcaaaca
    11521 tagtcggatg tcgacctttt tgattgccaa aagtataagt cgcaccttga tgaccacata
    11581 agggacttac gacatctgct aaaatatgaa tttgaacacc ttcaggaatt ttatagcaat
    11641 tttctgttga aacagaagct aagtttaata aggattgacc ggaagcaggc aagacatttc
    11701 catccctatc ataaaattga taacctaaac cagcagcaat ccccagtcct ccatcattac
    11761 tggccgtgcc accaacatcg atataaatat ctttaatccc tttagaaatg agatggagaa
    11821 tcaactctcc aataccacaa gtttggattt gaagtggatt tcgtttctct agcggaattt
    11881 ttccaagacc aaccaagtca gctacttcaa atagtgccag ttcccctttt tgaaaatagc
    11941 gcatggcttc tttttgtcca aaagggtctg tcacttggat ccatttttct tttaggtcaa
    12001 gagaatgtcg gatagcatct acagtacctt ctcccccatc accaacaggg cagaggagac
    12061 attctacatc tgctatcgat tgttggaagc ctctttttat tgcttcagct acctgttgag
    12121 ctgtcaagct ttccttaaac gaatccggtg caattacaat cttcatattt tccctcattc
    12181 taaacagtca atcaaaggaa gaacttctaa aaaatccctc ttgtcaacat gatgtggtat
    12241 ttcttttttg agcatttctt tggaacaaaa ggcgattcct aactccgccg acttcaacat
    12301 taatagatta ttgcccccat caccgattgc caccgttctt tctttagaaa gttttagttt
    12361 ctttctccat ttttccagag tttctttttt gacctgggga cttataattt gtccaactaa
    12421 ttttcctgtt aaaagacctt ctttgacttc aagctggtta gcagtgaaat aggcaatacc
    12481 aagggatttt gctaatctct ccaactattg gtgtaaatcc accagacacc agaccaacta
    12541 ggataccatt cttttggaga atagagatga actctgggac atttagcgat agatgaattg
    12601 agttgaagac gttatcaaag accaaaatag gaagaccttc caacaaggac actctttttc
    12661 ttaaactgct ttcaaagacc aactctcctc gcattgctcg acttgtaatc tgcgaaattt
    12721 ctgcctcatg acctgcctct ctccctaaaa gatcaatcac ttcttctagg attaaggttc
    12781 catctacatc caaaacacac aagcctttta cttgagacat cagttctcct ctctaaacag
    12841 cctaaaaatc gtatgaagtc atcatacgat tttatctatt aattaactaa actatggtac
    12901 aagtcaaggt atgacttgca ggctgtatcc catgagaagt cactctccat agcttgtttt
    12961 tgtaggtttc tccaaatgtc tggatggttt ctatacaagt ccaatgctgt ttggaaagtc
    13021 caatttaacc aataaggaga tagattgtca aagctaaagc cagtaccgct tccttcgatt
    13081 ggattgaaag cgcgaactgt atctcgcaag cctccaactt catggaccaa tggcaaggtt
    13141 ccataacgca tagccatcat ttgagacaag ccacacggtt caaaacgact tggcatgagg
    13201 aagaggtcac aagcagcgta gatttcctga gcaagtttga catcaaaagt gatatttgtt
    13261 gatagcttgt ctgggtaaat ctgagcaaac catgagaaag ctccttcaaa ggctggatcg
    13321 ccagttccca aaagaacaat ctgaacatct tcttgcaaga tatggtgaag actttcgacc
    13381 accacatcaa aacctttttg acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct
    13441 gctctaacag gcaagccaac tctttcttgc aattttgcct tatttttggc tttcccagac
    13501 aaatcttcct gattgaaatg atagtctaaa agagcatccg tctgaggatt ataaagatca
    13561 gcatcaatcc cattcacgat accagatact ttaccagact ccattttaag aatctgatcc
    13621 aaattacatc caaactgact agtcataatt tcatgagcat agctaggtga aacggttgaa
    13681 acacggttcg catagagaat acctgccttc atccagttca gacagttgtt ccatcgaagg
    13741 gtgccatcag cgtaacgttc aaagccaact ccaaacaaat cacccaacat tccttctgaa
    13801 aattgtcctt ggaattctaa attatgaatg gttaaaactg tttcaatgtc ctcataggct
    13861 tgaatccaac ggtatttttc cttcaacaag aaaggaatca tagctgtatg gtagtcatga
    13921 acatggagaa gatcaggaat aaagtcaatc ctttccatag cctcaatggc agccagttgg
    13981 aaaaaggcaa agcgttctcc gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa
    14041 tattgattgt caataaagta gaaggttaca ccatttaata ctgttttctt aattccacaa
    14101 tactgtctgc gccaaccaac gctcacctca aaatgaagca catcttcaat ctgatttcca
    14161 aatttagcct ctaccatatc atagtagggt aaaatcactg caacttcgtg cccagctttt
    14221 accagtgatt ttggaagagc gccaatgacg tctcccaaac cacctgtttt tgaaaagggt
    14281 gcaccctctg ctgctacaaa taaaattttc atgaatgaat atcctctgtt actttagcac
    14341 ctttcttaac cacaactgga tgttctgcag ttcctcgaat cacaacacca ggctcaactt
    14401 caaccccttt gtccaagata gcatattcga cctgagcccc ttctccaata acaacacgag
    14461 ggaagagcag gctatcttta accaagctat ccttatggac atggatatta cgtgatagaa
    14521 cagaattagc tacttgacct tcaataatac taccagaggc aaactgagaa gttcttacct
    14581 tagatgtatt agcatagtaa gttggctctt cgtttttgac ctttgtataa atcttttggt
    14641 ttggtgagaa aagagaatag aatttttgtg attcaagcat atcgatattc gcttgataat
    14701 aagatttaac agagtgaata ttggctagat agcccgtgta ctcgtaggcg aaagctccct
    14761 cttttacagc caaatcccgt aaaacatagc gcaatttctc tggatgttct tttttagctt
    14821 cttcttccaa gtgttcaatc aaccaaggtg tatcaacgac aaagatatct gtagacatat
    14881 tgaacgtttc agctgttgac ttgctatcaa agagtttatg agaaagaaca tggtctgttt
    14941 catctacatc caagattgca tttacttctg aaatatcttt cttagctagt tttttataaa
    15001 ctacagtgat aggctctttt gttgtactat gtaggtggaa aacttggttc aaatcaatgt
    15061 taataagaac atcgcagttg agggcaaccg tttggtttga gccagaacgt ttcaaataag
    15121 taagaagctg ttggtagtat tcttttccaa ctgtactact ttctacacgg gtattgtaaa
    15181 ttcctagata gtaatggcta agaagggttg ataagcccca ctcgcgtcct gaacgaatat
    15241 ggtcaaatac tgagctgata ttatcctgct ggaaaatacc aaagacacta cgaacacctg
    15301 cattagcaag gcttgaaagt gggaagtcaa tcaaacgata tttcccacca aatggcaaac
    15361 ttgctactgg acggtggtcc gtcaatgtcg acatattgtg aaaaccaact gtatttccta
    15421 aaatggcaga atatttatca atcttcatct gttgctaccc ccactacttc attatatcct
    15481 acaacttgta cttcatctgt tccatcaatt tcgacaccgt cagaaataat cgcaccttca
    15541 ccaataatgg cacgtttaat cttagctcct tgaccaatga tagctccact catgataact
    15601 gaatcaagga cttccgctcc ttcgcgaact tgcgcgcctg ttgaaaggat agaatgttta
    15661 acagttccat caacgaaaca tccgtctaca actaatgagt cttccacatg agcatttgcc
    15721 ccgaggaagt ttggtggtga aatcaagttt cttgagtaaa tcttccattg acggttacga
    15781 ctatccaagg cattttctgg agaaatatac tccatgttcg cttcccaaag tgactcaata
    15841 gtaccaacat ctttccaata accactaaat tcgtaagcat aaacactttc acctgactca
    15901 aggtaatttg gaatgacatt tttaccaaag tctgacatgc caaccttgct cttttcagca
    15961 gcgactaaca tattacgaag gcgttgccaa tcaaaaatgt agattcccat agaagctttt
    16021 gtagatttag gttgagctgg tttttcttca aattcaacaa tacgattgtt agcatctgtg
    16081 ttcatgatac caaaacggct tgcttcttta agagggacgt ctaaaactgc tactgtcaag
    16141 ctggcattat tatccttatg agactggagc atatcatcat agtccatttt gtagatgtga
    16201 tccccagaca aaatcaagac atactcagga ttgacactgt cgatatagtc gatattttgg
    16261 taaatagcgt gactagtccc ctcaaaccaa cgatttcctt cacttgcaga ataaggttga
    16321 agaatagaga cacctgaatt aataccgtct agtccccagc ttgaaccatt cccaatatgg
    16381 ttgttgagag caagtggttg atactgtgta acgaccccaa cattatgaat ccctgagttg
    16441 gcacagtttg atagggcaaa gtcaatgata cggtagcgcc caccaaattg cacagctggt
    16501 tttgcgatgc tttgagtgag tttaccgaga cgagttcctt gcccaccagc aagaatcaaa
    16561 gctaacattt catttttcat tttctactcc tttttggttt ttatttgtga cggttttagt
    16621 agatttcaag cgacgtttga ttttccatac acttgctccc atagccggta gggtaaaggt
    16681 taaggtctgc tcataatctt tccatagtcc ttcttgcgtt tgaacagttt gattatgttc
    16741 tttccaaacg cctccccact cttccaactc agtattccat acttcttcgt aaattcctgc
    16801 aacgggtagt ccgattgtaa aatctttccg ctcaacaggt accatgttaa agatacagac
    16861 taacatttct ccctttttac ccttacgaat aaaggaaaga acactctggt ctcgattatc
    16921 cgcatcaatg acttcaatac catcatagct ggtatcgatt tcccacagac agcgatgatc
    16981 tttgtaaaac tggtttagct gagaagtgaa atacttcatc ttagcattca ttgggtcttc
    17041 taggttagac cattccaact gttcttcaga tttccattct aggaattgac cgtattcgct
    17101 acccatgaag agcaatttct taccagggtg acaaatttgg tacgtataga gattgcgcaa
    17161 gcctgcgaat tgattgtaac gatctcccca catcttatgc atcatactct tcttgccatg
    17221 aaccacttca tcgtgcgaga atggcaagag ataattctcc ttgaaaacat acataaagct
    17281 gaaagtcacc aggttaaagt catatttacg ataaatcgga tcttcttcgt agaaacggag
    17341 gatatcattc atccagccca tgttccattt gtagtcaaat cctagaccac caatctcttt
    17401 cattcccgta atcttggttg cagacgaact ttcttctgca atcatcatca catctggata
    17461 ttctaactta ataacttcat tcaagcgttg aaggaaataa taaccttcat agttgagatt
    17521 tccgccatct ttattaggtg tccatggagc atcatcatag tccaaataga gcatgttgct
    17581 aacagcatcc acacgaatac catccaaatg atagatatca atccaatgct taatgcaaga
    17641 aattaagaag gactggactt cattttttcc aaggtcaaaa ttaagggcac cccaaccatg
    17701 gttatgagcc ttattatggt cttggtattc aaaagtcggt gtcccatcat aataggctaa
    17761 ggcatcatca ttgatggtaa agtgacctgg tacccagtcc acaataaccc caatattatg
    17821 ggtatgacac tcctcgacaa aatcttgaaa ctcctctggt ctaccatagg catgctctaa
    17881 agcgaagtaa cccataagct ggtaccccca actcaaacct agtgggtggg acatcaaggg
    17941 cataaactca atatgagtat agttcatctc aactaggtaa ggaatgagtt catctttcaa
    18001 ttgggcaaaa ctataaggac tgccatcagg atttctcttc caagagccag catgaacctc
    18061 gtaaatattg acaggacgtt cagcaaagcc ccaacgtttt cttcgtgcca gccaaagtcc
    18121 atccctccat ttcttatcag gaagctctgt tacgattgcc cctgttcctg gacgagcctc
    18181 atacctgaca gcaaaagggt caatcttcat cagttgatga ccattttgac gtgtgacatg
    18241 atatttgtaa atatgccctt cttgagccat attggtaaag acttcccaga ccccaaagtc
    18301 atttcttacc attggaatct gattttcaat ccaattggta aaatcaccaa ccaagtgaac
    18361 agcctgagca ttaggtgccc aaacacggaa ggtatagcca tactctccat ttagttcttc
    18421 cctatgtgct cctagataat gttggagata aaaattttca cccgtcataa aggtttttaa
    18481 tgcttctcta ttatccatat actccctttc tcctgtaagc gttttctatg tttttattat
    18541 actacctttt tagagaagat tcaagtaaat tactatactt ctttaattat tttgaaaatc
    18601 tacaacaagt tcacttactc gttcaattgt aaatcaatat tttttcaaaa aattgcgaaa
    18661 acacctttct ttttctacta tagtgaaatg aaataaaaca tgcgcaaatc gattaaggaa
    18721 tttaatctaa tttctaacaa tgtcttagaa atcaaagtgt actattttaa cttcaatgta
    18781 ctatatatag taaaatgaaa taataacagg acaaatcgat caggacagtc aaatcgattt
    18841 ctaacaatgt tttagaagta aatctgtact attctagttt caatatacta taataccaaa
    18901 aagttcgtat tttttctata attattatta ttttatagca aaaaataaaa ccaggaaaac
    18961 aatttcctga ttttacacgt tatttcacat caaaaacaat ggatttgaca tttgtcatcg
    19021 cttcaatgct atatttaatt ccttgcactc cagctccaga acctttgaca ccaaggaatg
    19081 ggaaattatc tggaccacgc tgggttttat tattaatgtg gactgtacct acttcaagtt
    19141 tttcagcaat ttcaaaggct tttttgaaat catttgtaaa gactgatgat tgaaggccga
    19201 attcagattc gttggcaaag gcaatagctt cctctacact agccacacga atgattggta
    19261 aaacaggacc aaatggctct tcccatgcca ctttcatatc ttttgtaact tggtcaaaaa
    19321 gcactggcca gaggagattg ccctcacgtt tgattggtgt aagagcctga gcctcttttt
    19381 cttgtgcatc ctcaatcaag ccccaaatga agtcggctga agcattgtca ataacaggtg
    19441 taatatcagc attgtcaaat ggatcaccaa ctgttaattt agaaacttct tcctgaagca
    19501 aagtagctaa tttatctgct acactttcga gaacaatgac acgtttaatg gccgtgcaac
    19561 gttgtcctga gtagctaaag gctcccgcaa caatttgctt ggcagcatgt tccaaatctg
    19621 catcttctag tacaagagct gcatctttcc caccaagttc caacatgata ggacgcatac
    19681 cagctaaacg accaatacgt tctccaatag gagttgaacc tgtaaagttg atgaagttga
    19741 cttctttgtg ctcaatgata taatccccaa tttctgaacc acgacctgta atggtgttga
    19801 aaacacctgc cggaatccct gcttcttcaa atgctttagc caacaagagt ccagaaatgg
    19861 aaccttgtgt tggtggcttc aacatgacca cattccctgc aatcaaggca ggtgcaattt
    19921 tagaagcaga taaattaact ggataattaa agggagcaat cgctagcacg ataccaactg
    19981 gttcacgacg gacaacagcc agtttgtttt tacttgctgc ctcaaaacca ccaccttcca
    20041 ttgcttgtcc agtgatacgg agaccttcct cagcagcata acgaatcaag tctgctgtac
    20101 gcactacttc tccaattgct gctttaatcc cttttgctac ttctttggca aggatagtac
    20161 caattttttc cttatcgcgt tctaaaatag ctgctgtttt atgcaaataa gccgcacgtt
    20221 caattgctga taaagctcgc catgctggca gggctgcacg cgcagcttgc atagcctcat
    20281 cagcttcagt ctgagtcatg gctggaactg tacccaattc ttcttgattg attggtgaat
    20341 aaatcgtaat ttcttgttca gatgatttcc attttccatt tactaaattc tgatatcttg
    20401 tcaaattctt gtcctcctga aaatgattaa gatatatttt accaaatttt cagaaaataa
    20461 caaccctttt tgcaaaattt ttgagattat tttcacaagc ttgatttttc aaagtatact
    20521 caataaaaat aaaaaaatcc actacgtcaa ggcgaggcta atgtggtttg aagaaatttt
    20581 cgaagagcgt gaatgagtat catctatagt aaaataaaaa aactgaacaa tttggttggg
    20641 gacagccaaa ccaatttctc acaatgtttc agaaacaagg gtgtgctatt ccaatttcag
    20701 cctactataa ctgtcataga ttgctgaaac aaagtctagg taaaagtctt cataataaaa
    20761 agacctccta tcaaatgttc aaaaactttg ataggaggtc ttgtttcgtg aaaatattta
    20821 tcaaattttc tatacaagtg agctgttagc caggttcttt ctattctttc aatttcaatg
    20881 aatggatttt ttaccaatac tcataactgg gaatttttct gtctaaaaat agcgagatag
    20941 atggtattta taaaacactc aagacagcta gactaatatc atttaaaaca ttatcttctt
    21001 ttgagcgact gttggttacc aacatagcta aatttcctgc attttcaaat tgatagggtt
    21061 ctgatttagc attcacaact accaagaggt gttctttgcc gtgaacttca tagataaggc
    21121 agccgctatg ttcaatcgca gaatgcacaa agacatgatg gtaaatttca tcatagctag
    21181 agtaagaaaa ggcaccagtt tttgtcttca atcggatgac ttgacggata aactcaatac
    21241 tgtcttgacg ctcattaatc aagttccagt tcacttggtt cacactgtca ggagcattat
    21301 agctattcat cgcacgctct ctatcatcat gggtcaactc accattttca ccagtcgcaa
    21361 ccagtttggt acgaccaaat tcttgaccga tttccataaa ggccatcccc tgcatgagca
    21421 gattcatggc tgtggcagtt tcgaccttgc gcatgatttg ctctgaactt tggtctggat
    21481 gaagggttgc caataaatcg tgaagattgt aattgtcatg ggcttctaca tagttaagca
    21541 cctgatttgg atgtgtatag cttcctaatt cacgacttcc taggattgct ttagctagaa
    21601 ttggctctgt cgcagcacca ctgacaaaac ctgacttgat agcaccataa acttctcccc
    21661 ctttgacagc atcgcgctga ttgtcattaa agaaaccaat atttggcatc tggtaggcat
    21721 tgtccttctt ggccttatca taaggggcaa gacctgttcc catatcccat ccttctccat
    21781 agaggataat gttggagtca atttcatcca agctttgacg aatcatctgc atggtcttga
    21841 catcatgaat ccccatcaag tcaaaacgga agccgtcaat attatattcc tgcacccagt
    21901 atagaagaga atcaatcata tacttgcgaa acatttcgtg ttcactggct gtttcatttc
    21961 caacacccgt tccattctgg aaggtaccat ctggattcat acgataatag taatcaggga
    22021 ctgttgtttg gaatggtgca tcaacaactg agaaggtatg gttatagact acatccataa
    22081 tgactccaat acccgcatcg tgataagctt gaaccatcac cttcaaatca cgaatgacct
    22141 gagctggatc atctggatta gttgaaaaac tagtttctgg cgcgttatag ttttgtggat
    22201 cataacccca gttgtaggtt acatttccat cctcatcgta ttctttatga cggtctgcaa
    22261 ttggttgcaa ttgaacataa ttgtagccca gcttcttgat gtaatcaaaa gcagttgact
    22321 ggccgtattg gttaactgtt ccagcctgag cagcacccaa gaaagttcct cgaagatgtt
    22381 catctacacc cgatgtaggt gatttagtca aatcacgaat atgcatttca cagataactg
    22441 ccttacatgg attttccaag cgccaagtag cctccgaacc gtgcttaacc tcgaagtttt
    22501 caacttgctt ttctacatgg ctcagaatag ctgaacgttt gccatcaggg ctggtcgcga
    22561 ttgtataagg atcacgtgtc agtgtttggt gatgagggaa ttggacttga tactgataag
    22621 tcttacctac caaatcttct tcaacatcca aactccagac accgattgta ttgtccttat
    22681 gattataaga gtagctattg cctcttttca tctcaaaagt cttccaaacg ggtgcatcat
    22741 tagcagctga ttcataaacg acaacttgca cttctgtcgc tgtaggtgac cagagggaaa
    22801 aatgagcctg attgtcctct acacggcaac ccaattctcc ttggtaaccc caatgatgat
    22861 caaaactagc actgttaatg gccttatcaa aggcaaaagg attttgattt ttatagaaag
    22921 gactggcaat agcaggattt tcagagtaat aaatcctatc atcgccttcc aaaatccaga
    22981 cctctgttaa taggggatag tgattaaaac ggatagaata ttctttacta gtttgacctg
    23041 tatgaaccac aaaattcaag ctttctataa catgtgaact tgggtgttca aagctaaata
    23101 aagctccaaa ataatcttct ttgtaggtta gcaaatcaat tcgttgatcc tgacttttta
    23161 caaaggagca agtgtcatat tctccattct tacgatggta atgaatgcgc atagggtagt
    23221 tatacatttt ttatttttcc tttttacttt gtttctattt cactaataaa tttttgtcaa
    23281 tctcgtctca attaacagac atagtcatat tctctaaact ctgtttttaa acgatccatt
    23341 acaaactttc tagccatgcc tcatctctga cctggatacc aagttcttgt gctttttgca
    23401 gtttacttcc agcgtctgca cctaccacga cgaggtcggt ctttttagaa acactacctg
    23461 tcactttggc acccagactt tcgagtttac ttttagcttc tgagcgcttg agtcgttcca
    23521 attttcctgt caataccacg gtcaaacctg acaaggccgc atccgctact accgtctgtc
    23581 ctttatagtc cagattgacc ccagtttctt tcaattctct gagcagaatt tcagagcctt
    23641 ctgtcgcaaa ataagtctga agacttttgg caatcacgcc acctagactt tcaatactag
    23701 ccacttcctc tgaatctgcc tgagacagat tttcaattga atggaaatat tgaagtaaaa
    23761 gctgactagc cttgcttccg acatgacgaa ttcccaaacc aaataagagc ttctcggcag
    23821 aattttcctt tgatgcttgg atagcctgat acagtttagc agcggacttt tccttaaccc
    23881 cctctaaaag gaggaaatcc tcttcttgca aacgataaat atccgccaca tccttgacta
    23941 aattagcagc aaaaagcttc tcaacaatag atggaccaag gcctgtaata ttcatagcat
    24001 cacgagaagc aaagtgaatc aagccttcca tgatttgagc agggcaacgc ggattgatac
    24061 aacgtagggc cacttcatct tcaaagtgca acaagtcaga gttacaactt ggacagtttg
    24121 tagggatatc tagtttttct tcagaaaccc gtttggactc taccacacgt aaaacggcag
    24181 ggatgatgtc accagcctta tatacaatga ccgtatcgtc ttttcggata tctttttcag
    24241 caatataatc tacattgtgc agggtcgcac ggctaacagt cgtaccggca agttgtactg
    24301 gtgttagatt agcagttgga gttacaacac cggtacggcc aactgtccag tcaactgata
    24361 agagttgagc ttctttttct tcagcaggga acttgtaggc tactgcccac tttggagcct
    24421 taacggtaaa accaagttct tcttgacttg ctaggtcgtt gaccttgatt accactccat
    24481 caatatcgta aggcagattt tcccgttcct gtcctacttc ttggataaaa ttccagattt
    24541 catctatgtt ttcagccaag attcgcttag gattgaccac aaaacctagt tgttctaggt
    24601 acttcaaacc cttttcttgg ctatcacgag ttgaagggct ggcttcttga tagagaaacg
    24661 ttgcaagatt acgcttggca actactgctg tatccaactg acgcagagtt cctgctgccg
    24721 cattacgagg attagcaaat tcaggctctc cattttcttg gcgcacttgg ttaacttggt
    24781 caaaggaagc gcgtggcatg taacattccc cacgaactgt gatatctagt tcttctggca
    24841 aagtcaaagg gatgtcctta acacgcttga ggttttctgt gatattttca ccaattgaac
    24901 catctccacg tgttacccca gcaaccaaaa tccccttttc ataagtcagc gagatagata
    24961 agccatcgat tttcagctca caaatatagg tcggatgagc cacttcctta cgaacacgcg
    25021 catcaaaagc atctagctcc tcacgtgaaa aagcatcctg caaactataa agaggatact
    25081 gatgactgta tttttcaaaa ccatctaaaa ccttgccacc aacacgatga gtcggactgt
    25141 ctgctagcac ttgctctgga taagcagttt ctaactcgac caactcacgg taaaggcggt
    25201 catactcact gtctgaaacc gagggattat cgctggtata gtactcagtc gcatagcgat
    25261 tgagcaaagc gactaactca ttcattcttt tattcataag accattttac cataaaacaa
    25321 gccctcctca caaacgagaa gggcggaaaa aacacttagt ttgaaattat ttttgaaact
    25381 caagcaacct tatatcaatt tttcaaaatg agttcgaaca taaataaacg atatacaaga
    25441 caagatgata acaccacttc caattatcag gaaagaagag agatgtacac ttggcaagac
    25501 tgtcataaat ccttttgcaa taggcataaa tagaatagct aaggtaaaaa ttgtactcag
    25561 tactcttcca agaaattcgc tctcaacctt ggtttgtact tgagtaaaaa agt
//
LOCUS       DAVBCR010000024        23935 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCR010000024 DAVBCR010000000
VERSION     DAVBCR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.024"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(225..2483)
                     /locus_tag="VQ508_000929"
     CDS             complement(225..2483)
                     /locus_tag="VQ508_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2489947.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2712..2942)
                     /locus_tag="VQ508_000930"
     CDS             complement(2712..2942)
                     /locus_tag="VQ508_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2489948.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3132..3815
                     /locus_tag="VQ508_000931"
     CDS             3132..3815
                     /locus_tag="VQ508_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2489949.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3815..4549
                     /locus_tag="VQ508_000932"
     CDS             3815..4549
                     /locus_tag="VQ508_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2489950.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4703..5560
                     /locus_tag="VQ508_000933"
     CDS             4703..5560
                     /locus_tag="VQ508_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2489951.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5702..6574
                     /locus_tag="VQ508_000934"
     CDS             5702..6574
                     /locus_tag="VQ508_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2489952.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6856..7539
                     /gene="rpiA"
                     /locus_tag="VQ508_000935"
     CDS             6856..7539
                     /gene="rpiA"
                     /locus_tag="VQ508_000935"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2489953.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7553..8764
                     /locus_tag="VQ508_000936"
     CDS             7553..8764
                     /locus_tag="VQ508_000936"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2489954.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIRESNKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8766..9308
                     /locus_tag="VQ508_000937"
     CDS             8766..9308
                     /locus_tag="VQ508_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2489955.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9324..10133
                     /locus_tag="VQ508_000938"
     CDS             9324..10133
                     /locus_tag="VQ508_000938"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2489956.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10609..11589
                     /locus_tag="VQ508_000939"
                     /pseudo
     CDS             10609..11589
                     /locus_tag="VQ508_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11614..12126
                     /locus_tag="VQ508_000940"
     CDS             11614..12126
                     /locus_tag="VQ508_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2489957.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12773..13483
                     /gene="deoD"
                     /locus_tag="VQ508_000941"
     CDS             12773..13483
                     /gene="deoD"
                     /locus_tag="VQ508_000941"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2489958.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13506..14030)
                     /locus_tag="VQ508_000942"
     CDS             complement(13506..14030)
                     /locus_tag="VQ508_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2489959.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14083..14319)
                     /locus_tag="VQ508_000943"
     CDS             complement(14083..14319)
                     /locus_tag="VQ508_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2489960.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14387..15307)
                     /locus_tag="VQ508_000944"
     CDS             complement(14387..15307)
                     /locus_tag="VQ508_000944"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2489961.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15497..15901
                     /locus_tag="VQ508_000945"
     CDS             15497..15901
                     /locus_tag="VQ508_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489962.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16117..16698
                     /locus_tag="VQ508_000946"
     CDS             16117..16698
                     /locus_tag="VQ508_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2489963.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16695..17972
                     /locus_tag="VQ508_000947"
     CDS             16695..17972
                     /locus_tag="VQ508_000947"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2489964.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17990..18652
                     /gene="deoC"
                     /locus_tag="VQ508_000948"
     CDS             17990..18652
                     /gene="deoC"
                     /locus_tag="VQ508_000948"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2489965.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18639..19028
                     /locus_tag="VQ508_000949"
     CDS             18639..19028
                     /locus_tag="VQ508_000949"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2489966.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19118..20170
                     /locus_tag="VQ508_000950"
     CDS             19118..20170
                     /locus_tag="VQ508_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2489967.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20314..21849
                     /locus_tag="VQ508_000951"
     CDS             20314..21849
                     /locus_tag="VQ508_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2489968.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21842..22900
                     /locus_tag="VQ508_000952"
     CDS             21842..22900
                     /locus_tag="VQ508_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489969.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22903..23859
                     /locus_tag="VQ508_000953"
     CDS             22903..23859
                     /locus_tag="VQ508_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2489970.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6949 a         4324 c         5453 g         7209 t
ORIGIN      
        1 tgctcttgtt tcaattgact atagcagatt gaaataagat gagaacaaat cgattgggaa
       61 agtaaaatta atttctataa atgttttagc aattgtttcg tactatttca gattcagtct
      121 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
      181 aaagaagcag aaaaatttaa ttttctactc cttttctata ggatttattt ttctttttca
      241 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      301 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      361 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      421 tcaaccaact tttccttgac cttgtcagtt acatccaaac gaatgttgtt gctagagagg
      481 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      541 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      601 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      661 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      721 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      781 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      841 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg agcgccgacc
      901 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      961 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
     1021 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
     1081 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1141 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1201 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1261 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1321 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1381 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1441 gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1501 gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg
     1561 tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact
     1621 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1681 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1741 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1801 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1861 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1921 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1981 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     2041 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     2101 tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct
     2161 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2221 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2281 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2341 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2401 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2461 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2521 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2581 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2641 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2701 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2761 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2821 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2881 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2941 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     3001 tagaaaaaac taagatttct cgctaattct gctagtttaa agatttttaa ataaattgga
     3061 taaaaaattt aaattttaat tcaaaacatg ttataatcat acagtattct attttagaaa
     3121 gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt aactatgggg
     3181 ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt ttgggtgttg
     3241 tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc aacttgtacg
     3301 tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt gctcttatgc
     3361 atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg cgtctgattc
     3421 cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagatt gttcgtgccg
     3481 gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg attcgtccta
     3541 aaaatgcgat gcgttatgtg attttgccac aagcagtcaa aaatatcttg ccagcattgg
     3601 ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt ggggtcatgg
     3661 agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta acaccacttt
     3721 tatttgcagc attttactac ttgattatga cctctattct gacagtagcc ttgaaagctt
     3781 ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga taaaaattga
     3841 aaatttacat aaatcttttg gaaagaatga agtattgaag ggcatcaacc tcgagattaa
     3901 aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta ccttgcttcg
     3961 ctctatgaat ttgttggagg aagcaaccaa ggggaaggtt atctttgagg gagtcgatat
     4021 tacggacaag aagaatgacc tgtttgccat gcgtgagaag atgggcatgg ttttccaaca
     4081 atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac ctatcaagac
     4141 taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg aaaaagttgg
     4201 tttgccagat aaggcagacg cttatccaca gagtctgtcg ggtgggcaac aacaacggat
     4261 tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg aaccaacttc
     4321 agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc ttgccaagtc
     4381 agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg tggcagatcg
     4441 tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc agatttttga
     4501 acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat ctattttaaa
     4561 atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt ttgaaactat
     4621 aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata atggaagata
     4681 tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt tagcggccaa
     4741 attgcagggg cagttggctg aaaagactgc aaaattaaag gaggaaacag ctctagtgcc
     4801 tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg ttcgcaacaa
     4861 ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc cagagaccat
     4921 tactcaagag gaattgttag acctgattgc taaatataat caggatccag cttggcatgg
     4981 gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt tattagccat
     5041 tgacccagaa aaggatgtgg atggtttcca tcccctaaac atgggacgtc tttggtctgg
     5101 acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc atgaatatgg
     5161 gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt tcaaatatcg ttggaaaacc
     5221 tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttgacccact cacgaactca
     5281 taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg gccgcgccaa
     5341 gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg ggatgaatcg
     5401 agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac cacttgctag
     5461 ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc tgatggagca
     5521 aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaagcatt cgattaatag
     5581 agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt ttagatataa
     5641 gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag aggaaaaaag
     5701 gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt ctcgttcaag
     5761 tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt atggaggtgc
     5821 catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattgatga ccgttggaac
     5881 ggacagggaa aatatcccgg ctctgcacag ccatttacct gagcctatgg ccttttctct
     5941 tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttatct tgctggggcc
     6001 tggtttagga gacaatgctt ttggagaaga tttagttaaa caggtctttg ctggcttaaa
     6061 acagaatcag attttgattg tagacggagg tgccttgacc attcttgcta ggacaagttt
     6121 gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg aaaaattatc
     6181 tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga cttccttccc
     6241 tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag ttggccaatc
     6301 tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa tgggagatac
     6361 tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc tctacgagcg
     6421 tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc aagaaaatta
     6481 tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa ttatttgtca
     6541 acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc tacttccttc
     6601 tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg tatcaaagtt
     6661 aagagtttgt ctttgtaact ggaaagagat gaacaaatca aatacataaa agataaactc
     6721 atttttattc gttttgtaaa aataaaatgc atatttttaa agaaagattg atgaaaaatg
     6781 tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag aactgagaat
     6841 gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc tgctgagttc
     6901 gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta ttttgtcgaa
     6961 gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac aacttctagt
     7021 gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga ccaagtagac
     7081 tttgtcgatg tgacggttga tggcgcggat gaagtggata gtcagtttaa tggaatcaaa
     7141 ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa agaatacatt
     7201 tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt gccagtagaa
     7261 gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg ctacaaacca
     7321 agtttccgtg aaaaagacgg tcaacgtttt gtgacggata tgcagaattt tatcattgac
     7381 ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga ccatgtcgtt
     7441 ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt tgctggacga
     7501 gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata agatgtctaa
     7561 atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag caccagatgc
     7621 taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg gacacatttc
     7681 aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa atattcctcg
     7741 tgaaactcct cttaagactg tagcagctga aagcaatcca actggatatg caacaaaatt
     7801 agaggaagta tctcttggta aggatactat gactggacac tgggaaatca tgggactcaa
     7861 cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc tgacaaaaat
     7921 cgaagaattc tcaggacgca aggttattcg tgaatccaac aaaccttatt caggaacggc
     7981 tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta tctatacttc
     8041 agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg atgaattgta
     8101 ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc ttggtcgcat
     8161 cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa accgtcgtga
     8221 cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg ctggtatcga
     8281 tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca accatgacat
     8341 gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta tgggacttgc
     8401 tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc tttacggcca
     8461 tcgtcgtaat gctcacggtt accgtgattg cttgcatgag tttgatgaac gcttacctga
     8521 aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc atggaaatga
     8581 cccaacgtat gcaggaacgg atcacactcg ggaatatatt ccattgttgg cctatagccc
     8641 tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt cagcgactgt
     8701 tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag ataaattggt
     8761 ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtggt aagaataagg
     8821 tcgtcatggc ggagcttcgt caagaattga caaacttggg actggaaaag gttgagagct
     8881 acatcaatag tggcaatatt ttctttactt cgatagattc caaagcccaa ttggttgaaa
     8941 agctagagac tttctttgca gtccattatc catttattca gagcttttct ttactgagtc
     9001 tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga gacttggcac
     9061 gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc gcgacagttg
     9121 aaagtttaga gctgaaagat gaagtgcttt attttggaaa acttgggatt ttctggggga
     9181 aattttctga agaatcctat tctaagactg cctatcataa gtacttgctg aaggtgcctt
     9241 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     9301 aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt
     9361 tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttgga tcaggacttg
     9421 gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact
     9481 ggggccgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc
     9541 gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg
     9601 tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg
     9661 cagctggcgg tatcggattt ggtcctggta ccttgatggc tatctcagac catatcaaca
     9721 tgacggggca aaatccattg atgggtgaaa acttggatga ctttggccca cgtttcccag
     9781 atatgtctag ggcctacaca ccagaatacc gtgccactgc ccatgaagtg gctaaaaaac
     9841 ttaatatcaa gcttgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac
     9901 cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc
     9961 ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta
    10021 actttgcggc cggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac
    10081 gtgttaaagg tgatttcaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa
    10141 gatttaaaag ggggagtgcc tctgtttttt caggattgac tgcctatccg gattaaagaa
    10201 gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata
    10261 ggtatgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata ctagcattag
    10321 cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat
    10381 ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac
    10441 gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa
    10501 attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt
    10561 tcagccaatg aagtaaagca agatgtaaca tctgaagtgg taataggtgt gctagattct
    10621 aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag
    10681 gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg
    10741 gaagagtcaa aagtagagat aacataacca gctcaagcgg atgatatccg caaggttgtt
    10801 ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga
    10861 tgttacctag ctcagattgc agcggttgaa gcttaccaaa aatatcctga tttttataac
    10921 catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt
    10981 tggaatgcta agaatggttt ctgggatgtt ggtttggaaa gtcgtaaatt agctgttagt
    11041 ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat
    11101 aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat
    11161 tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat
    11221 aaacatggtt atcgtgatcc gaaattggat aaagtgcgat tctttaagaa acaggctctg
    11281 cctcgatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag
    11341 gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc
    11401 gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg
    11461 caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac
    11521 actgcccagg atagattggc aaactttgat gtcattcttt tagacagttc tggtaaagaa
    11581 attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat
    11641 aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag tcttgcagaa
    11701 gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt ttctaagcca
    11761 gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga
    11821 gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa agatggaaac
    11881 caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc gggttttgtc
    11941 ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg
    12001 ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttc agctcaaccc
    12061 caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc
    12121 ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag tactacctga
    12181 gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc aatgaaaatc
    12241 aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga ggttgtagat
    12301 aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg tttgaagaga
    12361 ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca cgagtagaaa
    12421 acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac tgaatacggg
    12481 cgactctcct ctaaatcaaa attaagaaag gaattgaccc caccctaaaa gtagtgggaa
    12541 aaagatagtt ggtctagcga gcatcgctca ctgcgcccaa ctcctatttt cccttcgctt
    12601 tttgatgggt ttggtatctt tctcaatata aaataaagaa aggtagagcg tgtgttttga
    12661 tttgaacacg agcggaaaac tcggaaaata gataatctga ctgaaaaatc aggatttctc
    12721 gtcaggttcc taattttcag tcgttttctt ctcgctcttt gtatcataaa ttatgtctat
    12781 ccatattgct gctcagcagg gtgaaattgc tgataaaatt cttcttcctg gggatcctct
    12841 tcgtgctaag tttattgcgg agaatttcct tgatgatgct gtttgtttta acgaagtgcg
    12901 taacatgttt ggttacactg gtacttacaa gggtcactgt gtatctgtca tgggaactgg
    12961 gatgggaatg ccatctattt cgatttatgc gcgtgagtta atcgtagact acggtgtgaa
    13021 gaaattgatt cgtgtgggaa ctgcaggttc tttgaatgaa gaggttcatg ttcgtgaatt
    13081 agttttggcg caggcggctg caaccaactc aaacatcgtt cgtaatgact ggccacagta
    13141 cgattttcca caaattgcta gctttgattt gcttgataaa gcctaccata tcgccaaaaa
    13201 acttggtatg actactcacg ttgggaacgt tttgtcatct gatgtctttt actcaaatta
    13261 ctttgaaaag aatatcgagc ttggtaaatg gggagtcaag gctgtggaaa tggaagcagc
    13321 agctctttac tatcttgctg cccaatacca tgttgatgcg ctagctatca tgaccatctc
    13381 tgatagcttg gtcaatccag acgaagacac aactgcagaa gaacgtcaaa ataccttcac
    13441 tgatatgatg aaggttggtt tggaaacctt gattgcagaa taattatagc caaaaaggaa
    13501 gccctttatg aattgtagga cttccttttc ttatccagaa attgatctag ctctctctga
    13561 tttcgaagaa tagtgacttt atgtgaatat tcttggcaaa gtttttggta attttctttt
    13621 tgagttttgc tacgcccatc ccaaagaatc catctgataa actcccactc aaagcgttca
    13681 gggcaatctg ccgccatact ttctctgact tttccacggt atttaagata acgcttaaag
    13741 gctctaaaga gacaggtcaa tggcgaaaaa ttgagaaaga tgatttggtc agcttcttgc
    13801 attcgttctt ggtagtagca ccaagaataa ttaccatcga tgacccaagc tttatgcttg
    13861 gtgagaaagt tttttatctc ggttaacatc cattcgcagt cactgtcttg ccaaccaggt
    13921 tgaaattgga gtgtgtccat gtgcagtttt ggaatggagt agtagttaga taacttttct
    13981 gctatagttg acttaccaga accagaatat ccgataattg cgattttcat tttctacttt
    14041 ttcctatttg gagacaaaaa aacagcctct atggactgtt tcttatttag caagtttagc
    14101 tgaaagacga gctttatcgc ggcttgcttt gtttttgtga atcaaacctt tagtttctgc
    14161 tttatcgata gctgagctag cagcacggaa aagttcttca gatgggtttg cttcgaaagc
    14221 ttttatagca gtacgcatag ctgatttttg agctgagttc ttttcgtttt gtttaacgtt
    14281 caattcagcg cgtttgatag ctgatttaat gtttgccaat gttcttacct ccatatttac
    14341 taactatacc attatatatg aaaacttacg ttttgacaag ggaaaattac ttttttaagt
    14401 aaatttcatc gatttcatgg ttcttgcttt tatgaagaat cacttctgca cgatttctgg
    14461 ttggttcaat ataattttgc agatttgtga gattgatact gatccagacc tgatgggcaa
    14521 aggactccac ttccccaatc ggcatctgag taaaacgata atagtagcta tcagggtcgt
    14581 tttgggctag gctcagcatt ttcaagaaac ggtccagata ccaactttca atatcatcca
    14641 ctccagcatc tacatagatg gaaaagtcaa agaagtcagt gatatagaga cgatcgtttt
    14701 gtggattttg aaagacatta attccctcaa caattacaaa atcagcagct ttgacacttt
    14761 gtttcttttc gggtacgatg tcgtaaactt catgagaata gacaggaata tctacatctt
    14821 gtccattttt gatgcggtcc aagaagttga gaagagcttc catatcatag ctttcaggaa
    14881 atcctttacg atttaaaatc ccctgctcaa tcaaggtttg attgggatag agaaaaccat
    14941 cagttgtaac caactcaacc gtagcatctg taaacgtacg ggacagtagg atttgaagta
    15001 ggcgactggt tgtggatttt ccaacggcaa cactcccaga aaccccaata ataaaaggtt
    15061 gagatttact ttcacgttgg aggaaaattc cttttgaaaa ggctaaatct tccttagttc
    15121 gcttgtaaat ctgaatcaga tgagccaagg ggagatagat atctgtaacg tcttggagac
    15181 tgatttggtc attaaaactc ttgatagatt ccaattcttc ttctgtcaaa ggaggtgttg
    15241 tctttcgatg taaagattgc caagtctggc ggctgatttt ttcaaaatgt aaaaattcgt
    15301 tggtcatttg ttttccccta gatattttgt ctatcatacc ataaaaagct aaaaaaataa
    15361 agcggtttct tgaatgtggt aagtcaatgg tatatagtat aagtgatcaa tttattgatg
    15421 aaataggaga tagattctac tgatttataa gcttataaag ggggcttttt agttcacaac
    15481 ttataggagg tgtactatga aaatcttaaa acgttacata ttggaactct gttttatttt
    15541 aagttttgct ttacctttta taaaaggaac caatgcagat aatggtagat gctttgtgga
    15601 aacctattac ggttttactt ttttgatgga acatgctatt gtaacagctg tctttatcta
    15661 ttcgttttta attgctttct tactaaaaaa acgatgggcg aaatggattg ctgctggtag
    15721 ttattgcttt ttagtcctat ggattgcgac agagggttat ttcttccgca tgtcactaga
    15781 agatttgata cgactttgga caagtttgga aatcctgaca caaacgtatc agttgggctt
    15841 ttatctaaac attttgttag gaattttgtt gataataaag tattttaagg ttaagcaata
    15901 gaaataataa tctgcctgat tatagatgat aaactttttg tttttatctg gactgtgata
    15961 tttatagttc taaatggttt attatttata agtgttgaaa gagattgaag aaaatggctt
    16021 cgtgacttgc tttcttcggt ctttttcgtt ttattatcaa tttgtaaacg atttacaatt
    16081 tgagtcaaat tatggtaaaa tagtgtcatg agtaaaatgt attatgcaga aaatcctgac
    16141 gctgctcacg acattcatga gttgagagtg gacttgttgg gacaaaaaat gacttttttg
    16201 acagatgcgg gtgtttttag caagaaaatg gttgactttg gaagtcaact cttgctcaag
    16261 tgtctggatg tcaaccaagg agagacagtc ctagatgtag gctgtggtta tggtccatta
    16321 ggtttgtcct tggtcaaggt ttacggagtt caggcgacca tggttgatat taacactcgt
    16381 gccttggatt tagctcggag aaatgctgaa aaaaataatg caaaagcgac gattttccag
    16441 tccaacatct atgaacaagt tgaaggacat tttgatcatg tcatttccaa tcctcctatc
    16501 cgtgcgggca agcaagtcgt tcatgaaatc attgagaaaa gtaaagattt cttggaaact
    16561 ggtggggatt tgacaatcgt catccagaaa aaacaagggg ctccaagtgc taagtccaag
    16621 atggaggatg tgtttggtaa ttgtgaaatc cttaaaaaag acaaaggata ctatatcctt
    16681 agaagtgtga aagaatgaga gcagttgatt taatacaaaa aaaacgagac ggtcaagaac
    16741 tgacttcaag tgaaattgaa tggctggtag aaggctatgt gtcaggaact gttcctgatt
    16801 atcagatgtc tgcctttgct atggctgttt attttaaagg aatgacgact cgtgaaattt
    16861 ctgatttaac gatgaatatg gttaagactg ggcaagagtt tgacctgtca gccattgatg
    16921 gtgtgaaagt tgacaagcat tctactggtg gtgtcggcga taaggtaacc ttgattttag
    16981 ctcctcttgt tgctagcttc ggtgtgcctg tagcaaaaat gagtggtcgt ggtcttggcc
    17041 atacaggcgg aacaattgat aaattggagt ccattagggg ctatcaagta gaacgtagtc
    17101 aagaggattt tattcgtcag gttcaggata ttggagtatc tgtcattggg caatcagacc
    17161 agctggtcaa agcagataag cttctctatg cccttcgtga tgtgaccgca actgtcgaca
    17221 cgattccttt gattgcgagt tcggtgatga gcaagaaaat tgcggcaggg gcggatgcta
    17281 ttttgctaga cgtgactgtc ggtgagggtg ccttcatgaa gacggttgat gaggcgcgtg
    17341 agttggctca aactatggta gatcttggta aggttgttgg acggaagaca gtagcagtga
    17401 ttacggatat gagccagcct ttgggacgag cgattggaaa tcgtctggaa atacttgaag
    17461 cattggagat tttgcaaggt caaggacgtc aggatattac ccactttatt tgtgaattgg
    17521 ctcaaattat gcttggtttg gcaaatgtta acaagactgt tgaagaagtt caccaacatc
    17581 ttgagaatgg tcaagcactg gctaagtttg aggaaatggt gcaagcccaa ggtggagact
    17641 tggaagacct ctatcgtcct gtaaatgtag ctcatgtggt ggaaatccct gctcaggaaa
    17701 cgggtgtcat ttcagctctt ccagctatgg actttggtct ttatgccatg aggctggggg
    17761 ctggtcgtgc agttaagtct gatgccttgg actatgaaac aggaattgtt tttgaaaaga
    17821 agattggaga ctccgttcaa aagggtgaaa ttgttgcaaa agtatacaca aatgaaaaaa
    17881 ttccacctca actagttaca gatttccaaa aatgtgttaa aataagtgat gaagtgaaaa
    17941 aaatccgtga aattgtagaa atcatctcct aaatggaggg aaaaccgaaa tgaaattaaa
    18001 taaatatata gatcatacgc ttttaaaaca agatgcaaag aaaaaacaaa ttgatagttt
    18061 gttgtctgag gctagagagt atgactttgc cagtgtttgc gttaatccga cctgggttga
    18121 acatgctaaa aaaggacttg aaggcacaga tgttaaggtt tgcacggtag taggtttccc
    18181 tttgggagca acaacttcag ccgtgaaagc atttgagaca aaagaagcta tccaaaatgg
    18241 tgcagatgag attgatatgg tgatcaatgt tggagctctc aaatcaggta atttagcctt
    18301 ggttgagtca gatattcgcg cagtagtgga agcaagtggt gataagttag tgaaagtcat
    18361 tattgaagct tgccttctga cagaccaaga aaaaattgtt gtttgccaat tggcccaaaa
    18421 agctggggct gactttgtta aaacatctac tggtttttca actggtggtg ctacgatagc
    18481 agatgttaca ttaatgcgtg aaacagttgg atctgatatg ggagtcaagg ctgctggtgg
    18541 agctcgttct tatgcagatg ctcttgcctt tgtcgaagca ggtgcgaccc gtatcggaac
    18601 gtcagctggg gtagctattt taaaaggaga attggcagat ggcgactact gagttgattg
    18661 aactggcaat tgaaaccagc aaacatgcct atgtccccta ttctcacttt cctatcggag
    18721 cagttttagt agccaaagac ggaagtgttt acacgggagt gaatatcgaa aatgctagct
    18781 atcctttgac caattgcggt gagagaacag ctatttttaa ggctatatct gaaggccaaa
    18841 gagaattttc agaattgatt gtctatggtc aaactgaaaa accgatctcg ccatgtggtg
    18901 cttgtcgcca agtgatggct gaattttttg aacaagatct aaaagtgacc ttagtcgcaa
    18961 aagataaatc gacggtcgag atgacggtcg gggaattact tccatactct tttacagact
    19021 taaactagtc tgagtcgctc tctgagtggc acggtccttg tggccaacca atccatactt
    19081 gcaacatcgt tgcacatctt atttaggagg ttcagtaatg aacaagaaac aatggctagg
    19141 tcttggccta gttgcagtgg cagcagttgg acttgctgca tgtggtaacc gctcttctcg
    19201 taacgcagct tcatcttctg atgtgaagac aaaagcagca atcgtcactg atactggtgg
    19261 tgttgatgac aaatcattca accaatcagc ttgggaaggt ttgcaggctt ggggtaaaga
    19321 acacaatctt tcaaaagata acggtttcac ttacttccaa tcaacaagtg aagctgacta
    19381 cgctaacaac ttgcaacaag cggctggaag ttacaaccta atcttcggtg ttggttttgc
    19441 ccttaataat gcagttaaag atgcagcaaa agaacacact gacttgaact atgtcttgat
    19501 tgatgatgtg attaaagacc aaaagaatgt tgcgagcgta actttcgctg ataatgagtc
    19561 aggttacctt gcaggtgtgg ctgcagcaaa aacaactaag acaaaacaag ttggttttgt
    19621 aggtggtatc gaatctgaag ttatctctcg ttttgaagca ggattcaagg ctggtgttgc
    19681 gtcagtagac ccatctatca aagtccaagt tgactacgct ggttcatttg gtgatgcggc
    19741 taaaggtaaa acaattgcag ccgcacaata cgcagccggt gcagatattg tttaccaagt
    19801 agctggtggt acaggtgcag gtgtctttgc agaggcaaaa tctctcaacg aaagccgtcc
    19861 tgaaaatgaa aaagtttggg ttatcggtgt tgatcgtgac caagaagcag aaggtaaata
    19921 cacttctaaa gatggcaaag aatcaaactt tgttcttgta tctactttga aacaagttgg
    19981 tacaactgta aaagatattt ctaacaaggc agaaaaagga gaattccctg gcggtcaagt
    20041 gatcgtttac tcattgaagg ataaaggggt tgacttggca gtaacaaacc tttcagaaga
    20101 aggtaaaaaa gctgtcgaag atgcaaaagc taaaatcctt gatggaagcg taaaagttcc
    20161 tgaaaaataa tggatggaag tcatttctta ggaagcggcc aagggccgct tttttaagag
    20221 ttgagacaaa gtcattttgt ctcagtaaag cttgctacta gacaaactag caagttttat
    20281 tgaaataaaa taagactctg aaaggaagag cacatggcac acgaaaatgt cattgagatg
    20341 cgtgatatta ccaaggtgtt tggtggattt gttgccaacg acaaaatcaa cttgcaccta
    20401 cgaaaaggtg aaattcatgc acttttagga gaaaatgggg ctggtaagtc cacgctaatg
    20461 aacatgttag caggccttct tgaaccaact agtggtgaaa tcgcggtcaa cggtcaagtt
    20521 gtcaatctcg actccccatc taaagcagct agcttgggaa tcgggatggt tcaccagcac
    20581 tttatgttgg ttgaagcctt cacagtggct gaaaacatca ttttaggtag tgaattgact
    20641 aaaaatggtg tgctagatat cgctggagct agcaaagaaa tcaaggctct ttctgaacgt
    20701 tatggcttag ctgttgaccc ttctgccaag gtagcagata tctcagttgg agcccaacaa
    20761 cgtgtagaaa ttttaaaaac actttatcgg ggggctgata tccttatctt tgacgaacca
    20821 acggctgttt tgactccatc agaaattgat gagttgatgg ctattatgaa aaatcttgtc
    20881 aaagaaggaa aatcaattat cttgattacc cacaaattgg atgaaattcg cgcagtttct
    20941 gaccgtgtta cagttatccg tcgtgggaaa tcaattgaaa ccgttgaaat tgcaggggct
    21001 accaatgctg atttggcgga aatgatggta ggacgttctg tttcctttaa aacagagaag
    21061 caagcctcta aaccaaaaga agtggttttg tctatcaaag atttggtggt caatgaaaac
    21121 cgtggtgttc cagctgttaa aaatctgtcc ttggatgttc gtgctggaga gattgttggt
    21181 attgcgggga ttgatggaaa tggtcagtct gaactgattc aagccattac aggtcttcgt
    21241 aaggttgaat ctggtagcat tgagctaaaa ggagattcaa ttgtaggctt gcacccacgt
    21301 cagattacag aactaagtgt tgggcacgtt ccagaagacc gtcaccgtga tggcttgatt
    21361 ttggaaatga tgatatctga aaatattgcc cttcaaacct actataaaga accacatagt
    21421 aaaaatggaa ttttgaatta ttcaaatatt acttcttatg ctaaaaagct gatggaagag
    21481 tttgatgttc gcgctgccag tgaattagtt cctgcagctg cactctcagg aggaaatcaa
    21541 caaaaagcaa ttattgctcg tgaaattgat cgagatcctg atctccttat cgttagccag
    21601 ccaactcgtg ggttggatgt cggtgccatt gagtatatcc acaaacgctt gattgaagag
    21661 cgtgataatg gcaaggctgt ccttgttgtc agctttgaat tggatgagat tttaaacgtc
    21721 tcagaccgta ttgccgttat ccacgatggt aagattcaag gtattgtatc accagaaaca
    21781 accaataaac aagaacttgg tgtcttgatg gctggtggaa acttgggaaa ggagaagagt
    21841 gatgtctaaa aaattacaac aaatttcggt tcccttgatt tctgtatttc taggaatttt
    21901 actcggagcc attgtcatgt ggatcttcgg ttatgatgct atttggggct acgaagaatt
    21961 gttctataca gcctttggca gtctgcgtgg gattggagaa atcttccgtg ctatgggtcc
    22021 tctggtcttg attggtcttg gttttgcggt tgccagtcga gctggtttct ttaacgtcgg
    22081 acttcctggt caggctttgg caggttggat tctcagtggt tggtttgcgc tgtcgcatcc
    22141 agatatgccc cgtcccttga tgattctagc aaccatcgtg attgccttga ttgctggtgg
    22201 gattgtcgga gcgattccag gtattcttag ggcctatcta gggacgtcag aggttattgt
    22261 aaccatcatg atgaactaca ttgtcttgta tgtagggaat gcctttatcc atgctttccc
    22321 taaagacttc atgcaaagta cagattcgac cattcgtgtt ggggctaatg caacctatca
    22381 gacaccttgg ttggctgagt tgactggtaa ctcacggatg aatattggta ttttctttgc
    22441 catcattgcc gttgcagtta tttggttcat gctcaagaaa acaactcttg gttttgaaat
    22501 ccgtgcagtt ggtcttaatc cacatgcttc agaatatgct ggtatttctg ccaagcggac
    22561 tattatccta tctatgatta tttcaggtgc cttggcaggt cttggtggag ctgttgaagg
    22621 tttgggaacc ttccagaacg tctatgttca aggttcgtca ttagctatcg gatttaacgg
    22681 aatggcggtt agtttgcttg cggccaactc accaattggt atactctttg cagccttcct
    22741 atttggcgtt ctccaagttg gggctcctgg tatgaatgcg gcgcaggtac catctgagct
    22801 tgtcagcatt gtaacagcgt ctattatctt ctttgtcagt gttcattacc ttatcgaacg
    22861 ctttgtcaaa ccgaaaaaac aagttaaagg aggtaagtaa agatgtctat tataaccttg
    22921 ctccccctct tggtgtcttc tatgctgatt tactcagcac ctctcatctt tacaagtatc
    22981 ggtggtgttt tctctgaacg tggtggtgtg gtaaacgttg gccttgaagg aattatggtt
    23041 atgggtgcct tttctggagt tgtctttaac cttgaatttg cagaacaatt tggagcagca
    23101 actccatggc tatccttgct tgtagcagga ttggttggta gtgttttctc tatcatccac
    23161 gcagcagcga cggttcattt ccgtgcagac catgttgtca gcggtacggt attgaacttg
    23221 atggcgcctg ccttggctgt tttcttagtt aaagttcttt ataacaaagg acaaaccgac
    23281 aacctaagtc aaacttttgg acgctttgat ttcccagtct tggcaaatat cccagtgatt
    23341 ggtgatatct tcttcaagtc aactagtcta cttggttatc tggcgattgc cttctcattc
    23401 cttgcttggt ttattctctt taagactcaa tttggtcttc gtctccgctc tgtcggtgaa
    23461 catcctcaag cagcggatac cttgggaatc aacgtctaca agatgagata tttagggatt
    23521 attatttcag gttttctagg tggaattggc ggagcgattt atgcgcaatc aatctcagtt
    23581 aacttctcag tgacaactat tgttggacct ggatttatcg cccttgctgc gatgatcttt
    23641 gggaaatgga atccaatcgg tgctatgctt tctagtctct tctttggact ttcacaaagt
    23701 ttggctgtta tcggttctca attgccgttc ctacaaggag tgccagcggt ttatcttcaa
    23761 attgcacctt atgttttgac aattcttgtc ttagcagcct tctttggaaa agcagtcgca
    23821 ccaaaagcag atggtatcaa ctacatcaaa tcaaaataag catacaaaaa aacgtcagtc
    23881 caatgactga actgcccccc aaaagttaga cagaaaaaat ctaacttttg gggtg
//
LOCUS       DAVBCR010000025        23023 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCR010000025 DAVBCR010000000
VERSION     DAVBCR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.025"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..337)
                     /locus_tag="VQ508_000954"
     CDS             complement(<1..337)
                     /locus_tag="VQ508_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2489971.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSG"
     gene            complement(345..650)
                     /locus_tag="VQ508_000955"
     CDS             complement(345..650)
                     /locus_tag="VQ508_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2489972.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(637..831)
                     /locus_tag="VQ508_000956"
     CDS             complement(637..831)
                     /locus_tag="VQ508_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2489973.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(907..1247)
                     /gene="tnpB"
                     /locus_tag="VQ508_000957"
                     /pseudo
     CDS             complement(907..1247)
                     /gene="tnpB"
                     /locus_tag="VQ508_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1228..1413)
                     /locus_tag="VQ508_000958"
     CDS             complement(1228..1413)
                     /locus_tag="VQ508_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2489974.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(1717..3279)
                     /gene="guaA"
                     /locus_tag="VQ508_000959"
     CDS             complement(1717..3279)
                     /gene="guaA"
                     /locus_tag="VQ508_000959"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2489975.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3421..4119
                     /locus_tag="VQ508_000960"
     CDS             3421..4119
                     /locus_tag="VQ508_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2489976.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4162..5058)
                     /locus_tag="VQ508_000961"
     CDS             complement(4162..5058)
                     /locus_tag="VQ508_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2489977.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5067..6002)
                     /locus_tag="VQ508_000962"
     CDS             complement(5067..6002)
                     /locus_tag="VQ508_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2489978.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5999..6724)
                     /locus_tag="VQ508_000963"
     CDS             complement(5999..6724)
                     /locus_tag="VQ508_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2489979.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6808..7443)
                     /locus_tag="VQ508_000964"
     CDS             complement(6808..7443)
                     /locus_tag="VQ508_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2489980.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7445..8272)
                     /locus_tag="VQ508_000965"
     CDS             complement(7445..8272)
                     /locus_tag="VQ508_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2489981.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8294..8630)
                     /locus_tag="VQ508_000966"
                     /pseudo
     CDS             complement(8294..8630)
                     /locus_tag="VQ508_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(8631..9347)
                     /locus_tag="VQ508_000967"
     CDS             complement(8631..9347)
                     /locus_tag="VQ508_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2489982.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            9870..10481
                     /locus_tag="VQ508_000968"
     CDS             9870..10481
                     /locus_tag="VQ508_000968"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2489983.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10526..11254
                     /gene="rlmB"
                     /locus_tag="VQ508_000969"
     CDS             10526..11254
                     /gene="rlmB"
                     /locus_tag="VQ508_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2489984.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11293..12204)
                     /gene="trxB"
                     /locus_tag="VQ508_000970"
     CDS             complement(11293..12204)
                     /gene="trxB"
                     /locus_tag="VQ508_000970"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2489985.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12270..12494)
                     /locus_tag="VQ508_000971"
     CDS             complement(12270..12494)
                     /locus_tag="VQ508_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2489986.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12572..13315)
                     /locus_tag="VQ508_000972"
     CDS             complement(12572..13315)
                     /locus_tag="VQ508_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2489987.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13315..14115)
                     /locus_tag="VQ508_000973"
     CDS             complement(13315..14115)
                     /locus_tag="VQ508_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2489988.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14273..14629)
                     /locus_tag="VQ508_000974"
     CDS             complement(14273..14629)
                     /locus_tag="VQ508_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2489989.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14642..15154)
                     /locus_tag="VQ508_000975"
     CDS             complement(14642..15154)
                     /locus_tag="VQ508_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2489990.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15154..15648)
                     /locus_tag="VQ508_000976"
     CDS             complement(15154..15648)
                     /locus_tag="VQ508_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2489991.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15783..16229
                     /locus_tag="VQ508_000977"
     CDS             15783..16229
                     /locus_tag="VQ508_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2489992.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16226..16873
                     /locus_tag="VQ508_000978"
     CDS             16226..16873
                     /locus_tag="VQ508_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2489993.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(16894..17475)
                     /gene="pdxT"
                     /locus_tag="VQ508_000979"
     CDS             complement(16894..17475)
                     /gene="pdxT"
                     /locus_tag="VQ508_000979"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2489994.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17476..18351)
                     /gene="pdxS"
                     /locus_tag="VQ508_000980"
     CDS             complement(17476..18351)
                     /gene="pdxS"
                     /locus_tag="VQ508_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2489995.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18503..19882)
                     /locus_tag="VQ508_000981"
     CDS             complement(18503..19882)
                     /locus_tag="VQ508_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2489996.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20177..21100
                     /locus_tag="VQ508_000982"
     CDS             20177..21100
                     /locus_tag="VQ508_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2489997.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21160..21765
                     /locus_tag="VQ508_000983"
     CDS             21160..21765
                     /locus_tag="VQ508_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2489998.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            21783..>23023
                     /locus_tag="VQ508_000984"
     CDS             21783..>23023
                     /locus_tag="VQ508_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2489999.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6845 a         5158 c         4197 g         6823 t
ORIGIN      
        1 aaccacttcg acactgatca tggtggtaaa gcgtaatccc ttgtttctct gatttacctg
       61 acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga taagaggttt
      121 catctgcatg aagaagaggt tgttctaaca acttttctcg taaaaggtta taaagggact
      181 ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattagg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaagctg agtcaatagt ctcgttataa aggggcttta
      361 ggaataggag ctttcacaat tttatcactc ggatttttat cactgcatgc ttggcatttg
      421 taagcgtgtt ggatatgatc tatgcgtttt aattgcgcag gaataaagac taattcttgt
      481 cgttgaaggg ttgctccaat ctcttttaga tctccctgac aatcagagca aatgctctct
      541 tctacttgat gatgaacttc ttctgaatca aattgggcaa gaagagcttg gcgtttccct
      601 ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt
      661 ctcagatgga gactcttcct caaaaaggtt gagttgtcca gaggggcaaa cacttttctc
      721 agaggatttt ccatagagct tttgagtcag ataagccact tgttcacgaa gaagggcaag
      781 tgcattggtg agactatcaa ttgtagcact ctgttgttga atgattttca atcttcttcc
      841 atgggcttcc cctccttgtt ttctaccttt attatactaa aaagaaagtc ctcatttcaa
      901 tagaaatcac gactttctga tggatttatt tttggagtga tagaaaagtc cttcataagc
      961 cagtctactt gttcagatgt gagagctttg acatcctttt ctgtactggg ccaagtcagt
     1021 ctgccgttcc caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct
     1081 ttaaagcggt atttacgtcc accacaaaag agaaaaactt gacctgagaa aggatctaat
     1141 tcaaagtggg ttttaaccag ataagccagt gaatcaatgc cttgcctcat atccgttttc
     1201 ccacatacga gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt
     1261 atccaaaagc tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt
     1321 gccaatacga attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg
     1381 tggaatctct acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt
     1441 actaatagta tacaggagga actccatgaa tgatagatac cttgttatga cgcgattact
     1501 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
     1561 gcttgaaaac tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa
     1621 tttattcttt ttagtaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta
     1681 accgttgatt ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg
     1741 tggtttactt gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg
     1801 tactgagatt ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc
     1861 gatagaagtg atagcacgga ttgcaatcgt gtagtcatat gtacgaccgt cacccataac
     1921 accgactgaa cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc
     1981 agctttagcg atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc
     2041 ttcagtgatt tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca
     2101 tacgatatgg tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag
     2161 agtattgagt ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg
     2221 gtgtgacttg atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt
     2281 accttgagca aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac
     2341 aaactcgtta ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc
     2401 aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat
     2461 gtccataact tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat
     2521 caattgatcg ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc
     2581 tgatagacca agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat
     2641 gtcaatgaaa ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt
     2701 acgaaggata tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta
     2761 aatgtgttta tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg
     2821 aacaaagtca gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc
     2881 atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc
     2941 acgatttcca gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg
     3001 cataccataa cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc
     3061 aaatgaacca tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt
     3121 gacttcacga acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat
     3181 ctcacggata cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat
     3241 tttttctaca tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa
     3301 atatcttttc tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag
     3361 tttgactttt tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat
     3421 atgctacctg cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt
     3481 tgggctattg gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt
     3541 cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt
     3601 gtaggaagcg gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc
     3661 agttttactg aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac
     3721 aaaaaaacca ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat
     3781 attatccgaa tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct
     3841 tctattcctg aaaaattcat taaggacttt aaaaaagaag aaatcacaag tcatttcttc
     3901 caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc
     3961 cttgctaaag aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga
     4021 ttgacccagg tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat
     4081 gtaggcgaac gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct
     4141 gtcacggaca aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt
     4201 tacagcaaac aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat
     4261 tattaagaag gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt
     4321 gaccggtcct atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat
     4381 aaggataatg cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag
     4441 aacccctgta aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgagcaa
     4501 gctgctcccc cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa
     4561 ggcagaaaag agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc
     4621 atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag
     4681 tgtcggtgcc accctatcct tgatacagct ataaattaaa attccgacag gacaaacata
     4741 ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg
     4801 gttgagaaaa agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt
     4861 taaaaagacc agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc
     4921 agcgattaaa agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta
     4981 ttggccactc gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc
     5041 ttttaaacta tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga
     5101 ttgcggtcta atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa
     5161 atagtggctt cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg
     5221 tctagccaat ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa
     5281 aaattttgat ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt
     5341 aaatccttta tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc
     5401 ttgggatcat gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact
     5461 ggcccaaact tggtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca
     5521 tccaaatctt gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc
     5581 gaataaagaa aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca
     5641 ggggctgtta aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag
     5701 agctggcgaa tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta
     5761 tcaatatcta attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc
     5821 ttgctgacac tagctagatc ataaactagt cctgcctcag tctgcaagcc atgctctggg
     5881 tcactctggc ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct
     5941 ccgggataaa ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc
     6001 atacttcttc aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag
     6061 acttaggaat aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt
     6121 cattgaccaa gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat
     6181 cctgcccctg agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac
     6241 tttcgatatc agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg
     6301 atgccaaatc tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat
     6361 cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat
     6421 tttgaccttt atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta
     6481 agggattttc caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag
     6541 tacgcatact gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg
     6601 acagaaaggc cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt
     6661 catttaattt tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat
     6721 tcataaattc ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta
     6781 tgctcaaatt taacattatt tagctattta gtaaagatag tctttcaagg attttgacaa
     6841 agcctgataa ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg
     6901 gcctgcttgg tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga
     6961 tgcaagctct tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac
     7021 tgcctgctgg tactcctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt
     7081 caatggataa tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt
     7141 cacaggaaca tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc
     7201 tccaccatat agatgagcat caagattctc tagtaacagt cctgtctgat agccacgaat
     7261 tcctcgattg acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac
     7321 aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg
     7381 atacttagtt tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc
     7441 cacttcattc tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt
     7501 tggctggtgc tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat
     7561 aggcaattcc agccatttca agcatttcaa catcattttc actatcacca aaagccatga
     7621 tttcttggga tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa
     7681 tcccagcttg gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat
     7741 caaagagagc attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc
     7801 tcatcttgag cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt
     7861 ggtagaattt ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac
     7921 cttccttgac aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac
     7981 ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca
     8041 attcattgtt ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca
     8101 agggactcag tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa
     8161 tgcccctttg atccaactga tctaaaatct tttccaatcg tgggagatca agctgacccc
     8221 tagcatccag caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc
     8281 ctctggattc aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg
     8341 tatcaattcg ttccttagtc gcttcatcta actggtcacg atgactgcca cccctctata
     8401 aaatcttcca aaatataagt ttggtaatga tacaagggat atccttctgg tgaaaaggtt
     8461 ccctttggtg ttcgattgac ccaagtagga tgagctttag ctgttccgat agttgttttt
     8521 ccatccttct tcttgatggt gacatccatg agaacaccac gttcagtcca cttagcattc
     8581 tcttcatctc ccatagattc aatccgttga ttggaaatga agttccccat ttaatagata
     8641 atgagtttct tatctccatc tttttcaacc gttttagatg gttcaacaac gtgaggatgc
     8701 cctccaaaga taatatccgc tccccaatcg atcatcttgt gataaagagc tttttgttct
     8761 tcagttggtt ccaatcgata ctcaacaccc atctgaggca tgataatggt gatatctgct
     8821 tccttctctg cccgttcgac ttcagccttt atcttatctt cgtttaaatc tgaaagataa
     8881 cgattatagt cttcctgaaa aatatactgc tcaattccat tgaaaccata ggaataggct
     8941 aacaatgcaa ccttgatagc attcacttcc ttaatgacca gcggagcctg atcacgtggt
     9001 tcgtgcgtat aaactccgat tggagtgatt ccagctttct caataatatc ggccattgaa
     9061 ataactccct caatttgcga atccaaaata tgattatgag ctaaatccag cacatgataa
     9121 cctgcttcct taatagcatc cataacttca acaggagcat taaagagaag ataacccgct
     9181 aaataatgat ccttattaat ggttccttca aaatcaccaa tagctaaatc tgcttgcttg
     9241 agccaaggag tcacatactc aaaattttca tgaaagtcat aggtaccgtc ttctttttta
     9301 gctgaaaaga aaagtccatc gtggtagagc aaatccccgt gggccataat tctggcagtt
     9361 ttttcctctt cctactcctg agacttttgc ttagtcccct cttgagaaat agtatctcgt
     9421 ttttgactag tcaagggatt ccttggaagc tttcaaacaa caagaccaag cccattgata
     9481 aaccaactgc tagcaagagt atcgccacaa atcccttatt gctccacttg cgataactcc
     9541 taaaaagttt accaagccct tcataaaacg aaaagctaaa ccaccctgat ttcgattttg
     9601 tcttctttgt atcttcattc tccctacttt cttatgcaag ccttttcttt ttattatatc
     9661 acagataagt atttctttca caattgaatt gaacttccca tctattttct ataaatccta
     9721 aatgccataa tgctttcaat tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga
     9781 aatgaactaa tccacagtgg cttattccaa gtataccact tgggctttgg cagtagttaa
     9841 ctgcactaaa cataatataa ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg
     9901 ctcacttaat tgatatgaac gatattatcc gtgaagggaa tcctactcta cgcgcgattg
     9961 ctgaggaagt cactttcccc ctatctgacc aggaaatcat cctaggcgaa aagatgatgc
    10021 aattccttaa acattcccaa gatcctgtca tggctgaaaa aatgggactc cgcggtggtg
    10081 ttggactggc tgctccccag ttagatatct caaaacgcat tatcgctgtt ttggtaccta
    10141 atattgttga agaaggcgaa actccacagg aagcctacga tttggaaggc attatgtaca
    10201 atccaaaaat cgtctctcac tctgttcaag atgctgctct tggcgaagga gaaggttgcc
    10261 tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt actgttgact
    10321 actttgacaa agatggagaa aaacaccgta tcaaactcaa aggctacaac tccattgttg
    10381 ttcagcatga aattgaccac attaacggta tcatgtttta cgatcgcatc aatgaaaaag
    10441 acccatttgc agttaaagat ggtttactga ttcttgaata aagaaaatcc cgttgcaaga
    10501 cggggttttg tgttataata gaggcatgaa aacaaatgat attgtctatg gtgtccacgc
    10561 cgttaccgaa gccctccttg caaatacagg aaacaaactc tacctccaag aagatctccg
    10621 aggtaagaat gttgagaaag tcaaggaact agctacagaa aagaaggtgt ccatttcttg
    10681 gacatcaaaa aaatctctct ctgagatgac tgaaggtgct gttcatcaag gttttgttct
    10741 acgagtgtct gaatttgcct atagcgagct agattacatc cttgcaaaaa cacgccaaga
    10801 agaaaatcca cttctattga ttctagatgg tctaaccgat ccccataatc tgggttctat
    10861 cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc attcccaagc accgtgctgt
    10921 cggagtaact cctgtcgttg ccaaaacagc cacaggtgct attgaacacg ttccaattgc
    10981 ccgagtgaca aacctcagtc aaaccttaga taaactcaag gatgaaggct tctggacctt
    11041 tggaacggat atgaacggta ctccttgcca caagtggaat acaaaaggga aaatcgccct
    11101 catcatcgga aatgaaggaa aaggcatctc tagcaacatc aaaaaacagg tcgatgaaat
    11161 gattaccatt ccgatgaatg gacatgttca aagcctcaat gccagtgttg ctgcagccat
    11221 tctcatgtac gaagtttttc taaatagact ataaaaaagt ttccagtcat ctgattggaa
    11281 acttttttat gattaactat gttctgtgat aaacttgtag gcttcttgac cagcgatagc
    11341 tccatctcca actgctgttg ttacttggcg aaggtctttc aagcgaacat ctccaactgc
    11401 aaagataccg tcgactgcag tcttcatgtg gctatctgtc acaatccagc ctgcctgatc
    11461 ttggatattc aattctttaa caaaatcgct aagagggtcc aaaccaacat agataaagac
    11521 accaccgaag gcttgctctg tcacttgacc tgttttcaca ttttcaaaaa cgacagattc
    11581 tactctgttt tcacccttga tttccttcac tacagaatcc cagataaagc tgattttttc
    11641 attcgcaaag gcgcgatctt gtaaaacctt ttgggcacga agttggtcac gacggtgaac
    11701 aatggtaaca gtcttagcaa aacgagtcaa gaagagggct tcttcaacag ctgaatctcc
    11761 accaccaact accaataaat cttggtcacg gaagaaagca ccatcacaca cagcacagta
    11821 agaaacacca cgactgttca gttcttcttc tccaggcact cccaaaggac ggtgtttaga
    11881 accagttgct acgataactg tacgtgtttc atatgtttgg tcatcagtca tcactttctt
    11941 aaaatcacca tggtcttcga cattttcaac ataaccataa atgtgctcaa caccaagatt
    12001 ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca ctaatattag cgtatcctgg
    12061 gtaattttcg atatcagatg tattattcat ctgaccacct ggcagaccac cttcaatcaa
    12121 agctactttt agattgcttc gagcagcata caaggccgca gtcatccctg caggtccagc
    12181 accgataata atagtatcgt acatatagat tccttctttc ttgttgtaac tatctttatt
    12241 ctaactcttt cttgtcaatc acgcaaggat tatgccttga aaacaagaat taaactcata
    12301 accgcaaagg ataatactgg gacaaccaag actccaatca taatcatatc atagagatgg
    12361 gcttggcgag ccctggctgt cttatcaact cccgacatgg ctctcagacc aatccaaatc
    12421 cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac tctcgaaata taaagaaaat
    12481 agttgcagta gcatgatttc tctcatttct atctttttta aagagtaaac tcagctagtc
    12541 caactaactg agttttcctt tatctattat atcaaatata agtccgtttg taactagcga
    12601 agaattcttt tgtccgctct tctttagggg tgtggataat ctcatccgga gttccagact
    12661 cgatgatttt ccccttatct aataagagaa ttttatccgc aacttgggct acaaaggaca
    12721 tgtcatgact gaccaaaatc atggtctgac ctgacttagc agcatctgca atagactttt
    12781 ctacttcacc gaccaattct gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat
    12841 ctggtttcat agcaagcgca cgcgctaggg caacccgttg cttctgtcca cctgataaat
    12901 ggcgaggata atggttttca cggtccgaaa gcccaacctt agccaactct tccttggcaa
    12961 tcttagtcgc ttcttggtca gataatttct tgacaacaac caagccttct ttcacattat
    13021 caagtgctgt tcgacgttca aataaattaa actgttggaa aaccatagac aacttacggc
    13081 gtagggcaag gatttcttct tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct
    13141 gaatagagcc actgtcaggt gtttcaagat aattgagact gcgaagaaag gttgattttc
    13201 cagctcctga agaaccaatc aaggctacaa cttccccttt ttgaatatcc aagttcagat
    13261 gatccaagac agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta atcattaacg
    13321 aaggtctcct ttcacatctg tttgcactgt atcaggtgca gaaatagcca tttttctctc
    13381 gatgaaacga ccgaggcttt caattccgat attgactacc caataaacaa gggcaacaga
    13441 gatgaagcgt tcaaaatagc gataatcagc tccacctaga atctgagctt gggcaaagac
    13501 ttccacaaca cccgcactaa aagctagaga tgttcccttg gtcaaaccga tgagggaatt
    13561 aatcaaggtt ggagtagcta ccaccgctgc attaggaata atcactcgtc gataaacttg
    13621 cgctcgggtc atacccagac tgcgcgccgc ctcaatctca ccaggattaa ctgagagaat
    13681 ggctgcacga atggtttcac tagcataagc tgcctcatta aaggcaaaag cgacaatcgc
    13741 aaaagctgca gctggaatcg cattgatatt gagaccagtt ccccattgct gattgagggc
    13801 tttcaaagcc aaagggattc cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc
    13861 ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa atcttgacac gattgatctt
    13921 cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat
    13981 aattgttgtt ggaagttgtt ggacaattct agggattcca tcaaagaccg aacgtaggct
    14041 aaacagcttg ccatccggaa tcaattgcat caagttttgg taccaatctg atgctaaaat
    14101 cgttgtaaca ttcataaaaa catcctcttc tgataatgat tcattctacc atgcttctgc
    14161 cgtcaatgaa attatttgct acggtcagat acagactttg tctacctact agtttatcat
    14221 actttgaaac agggaagtta tatattttat ctatcaaaga gatagataaa aactatttca
    14281 gtcccagttc ttcataagat tttcgataac cgatttgctt aacagttcca ttttctacta
    14341 aaatgggccg ttttaacaac ataccgtcac ttgctagcaa ctcagccgct tcttggtttg
    14401 acaaacttcc taccttatct tttagcccta attcacggta tttgatacca ctggtgttga
    14461 aaaattgctt caattcaaat cctgaggttt ctagccaatt caaaatgact tcttggctag
    14521 gtgtttcttc cacgatatgg acggctttat agtccacacc taattgattt aattcttgtt
    14581 ttgctttttt acaagttgaa catttggggt attcgataaa ttctaacatg tgcttctcct
    14641 tctatttgtt tatatcttta aaatctacgc cttcatgctc caagagccaa gctttctttt
    14701 ccactcctgc agcatagcct gtcagacgct tgcctgctcc caacacacga tgacaaggta
    14761 ctaggataga ccaaggattg cgtcccactg ctccaccaat tgcttgagca gaagccactt
    14821 gcaggtcttg agcaatttgt ccataggtca ctgtctgacc ataaggaatg ccctgtaaat
    14881 agtcccaaac tctcttttca aaattcgttc cgattggcgc caagagcaag ttggataaat
    14941 cctgaggctt gcctttaaag taatcatcta agcaagcaat aactgggtct aaaataggat
    15001 gactaacaac ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc ttctgctcct
    15061 gaacccaaat tccatacaaa taatggtcat cagctacaag tgatagaata ccaattggtg
    15121 aggagtagag tatttttaca tacttctttt gcattatttc tttaattttt gataggccaa
    15181 gcgttcgcca tctactggca ccttaccaac ttgtttaaaa ccaagttttt caaaaatatg
    15241 ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa tcaagataat caaaaccttc
    15301 aatcaagccc tctaagaagg tttgagcaac tccttttccc tgcacatctg ctgccacagc
    15361 aatacggtga aagactagat actctgactc tccagcttgc cagtttccct cataaatagc
    15421 ttcataggcc tcctctggac tcttggtcac agcagcatag gctagcagtt ctccctcttc
    15481 caaggctacg taggcttgcc ctgagatgat atcatcgata ataacatctg catttggata
    15541 gccattttgc cactggtcac taccagcatc tgctaaacat tttttggcat cctccatcac
    15601 ctgcatgatg gcatctactt catttggaaa agctaaacga atttccatct tttttcctca
    15661 tttctgacta gtttctccca tcatagcata atttaagtat ttctacaagc agttaaaact
    15721 tgactttgtg tttttgttat tttataatct agttatcaaa actatataaa aaggagttga
    15781 aaatgaaaac taccttttcc tacccaaaat gggcagaaat tccaaacatt gacctctatc
    15841 tggaccaggt tttgctctat gtcaatcagg tctgcgcccc tatctctcct aataaagaca
    15901 agggcctaac agcatctatg gtcaacaatt atgtgaaaaa tggttacctg acaaagcctg
    15961 acaaaaaaaa ataccaacgc caacagattg cccgtttgat tgctatcaca accctcaagt
    16021 ctgtattttc tattcaagaa atagcccaga cacttaatac tctacaaact caagcaagtt
    16081 cagaccaact ctacgatgct tttgtggact acatgaacca aggaattgac ccagctaacc
    16141 ctattatcca aaccagctgc caaaccgtca aactctatca tcaaactcta gacttaatcg
    16201 atcatactca agaggaggta atccaatgaa cactagtctt aaactcagca aacaactcag
    16261 ttttggagag gagattgcta atagcgtgac ccatgctgtg ggtgcagtca tcatgcttat
    16321 cttgctgcct atttcatcca tctatagtta tgaagcacac ggatttttat cctctatcgg
    16381 cgtttccatt ttcgtcatca gtctctttct catgttccta tcatccacca tttatcactc
    16441 tatggcctat ggttcgaccc acaaatatgt tttgcgaatc attgaccatt ctatgattta
    16501 cgttgccatt gccggctcat acacgcccgt tgtcttgacc ttgatgaata actggtttgg
    16561 ctatctgatt attgtcatcc aatggggaac gaccatcttt ggtattctct ataaaatctt
    16621 tgctaaaaag gtcaatgaga aatttagcct tgctctttac ctgattatgg gctggttggt
    16681 tctggctatc attcctgcca ttatcagtca aacgacaccc gttttctgga gtctcatggt
    16741 aactggcgga ctctgttata cagttggagc tggattttat gccaagaaaa aaccttattt
    16801 ccacatgatt tggcatctct ttatcctagc tgcgtccgca cttcaataca tcgctattgt
    16861 ttattacatg taaaaaagtt gagaaattca atctcaactt ttttctttac acatattgat
    16921 aaagtactgg tgcaagcgca catcatcagt caattctgga tgaaaagaac ttaccaacat
    16981 atttttttct tgggctgcaa caatttgatt gttcactgtt gctagaattt ctacaccctc
    17041 accaacacta ctgataatcg gaccacggat aaaggtcatt ggaatcttgc caactccctt
    17101 acattctgct tccgtgtaga aacttcctaa ttggcgccca taagcattac gctcgaccac
    17161 catatccata gttcctagat gactctcttt ctgagaagtg atttccttag ccagcaaaat
    17221 taagcccgca caggtcccaa acactggtaa gccagataga atggcttctc ggatgggaag
    17281 tagcatgttc tggtcacgta agagcttgcc catggttgta gactcaccac caggcaaaat
    17341 caaacccgac aagtcactct gatcttgctg aaaatcatct agatttctga gttctacact
    17401 ctcgacacct aattgatcta gcacttttgc atgttctgca aaggcccctt gcaaggccaa
    17461 tattccaatt ttcatctatt ttcctcgttc agccatgaga atttggattt cattttcatt
    17521 aataccaacc atggcttctc ctaaatcttc agagatttga gctaggattt gaggattacg
    17581 gaagttagtc acagccttaa caatggcact cgctcgttta acaggatctc ctgacttgaa
    17641 aatacctgaa ccgacaaaga ccccctctgc ccctaattgc atcattaacg cagcatctgc
    17701 tggcgttgca acacctccag cagcgaaatt tacaactggc aattttccat gttcatgaac
    17761 atattggacc aattctacag ggacttgcaa atccttggca gcaacataaa gctcgtcctc
    17821 acgtaagttt tgaatgcggc gaatttcctg attcatcata cgcatatgac gaacagcttg
    17881 gacgatatcc cctgtccctg gttctccttt ggtacgaatc atggaagcac cttcagcgat
    17941 acgacgcaag gcttcaccca aatccttagc accacagaca aaaggaactt ggaattcttt
    18001 cttgtccaca tggaaacggt catcagctgg agatagaact tcactctcgt cgatataatc
    18061 aatttcaata gcctctaaaa tctgagcttc aacaaaatgc ccgattctga ccttagccat
    18121 tactggaata ctaaccgctt cttggatttc cttaatcatc tttgggtcgc tcatgcggga
    18181 aactcctcca gctgcacgaa tatcagccgg aattcgttcc aaggccatca cagctgccgc
    18241 accagcagct tctgcgatac gagcctgttc aggattctgc acatccataa taacaccacc
    18301 cttgagcatc tgtgccaagt ttttatttag ttcataacga ttttcagtca tttgttttat
    18361 cctcatcatt tgtattggta gctacctttt cattttaagt cagaacagaa tgaagcacaa
    18421 gataaaaaaa ctataattgt atggggacag attggctaaa aactatctga ccttaactta
    18481 aggccagata gctcattcat ttttattttt cagccgtaag ggcagccatt gtgatgtagt
    18541 tgtatggttt attgaagtgt ggcaagaaga agaggtctgt caatgccaat ttatcaattg
    18601 tcacatgctc ttggatagca agtgagaaca tgtggattcc catgctaatt gcaatatcat
    18661 gtgaaaccat ttgggcacca agaatttcac ggctatcttt gtcaaagaca atcttaattg
    18721 ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta aagcctgttt
    18781 cagttgcgtt gtaaccagca gctttcgctt tttcaagagt caaaccagtt gaaaccatgt
    18841 gaagaccgta gattgagata ccatttgaac cttgaacacc gattccttcc aattcatgtc
    18901 cacaagcgtt gtaggcacca acgataccag tgcgaacagc atttgaagca agagcgatat
    18961 agcttgtatc tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca gcgtaaacgc
    19021 ctgggataga tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg aagagttcga
    19081 tcttaccatc agcaagggct gtgtttggac ggaaaccaac tgcaaggata accatatcca
    19141 cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt accatcacct tcgattgctt
    19201 taacagtttg acctagagcc aagcggatgt tgtgatcttc caagttcttc gccatcattt
    19261 gtgtgaagtc tttgtcatag taaccgttca agacagtatc aacgatatca acaaggacaa
    19321 cttcttttcc aagacgttca aaggcttcag caagttcaac accgatgtaa ccaccaccaa
    19381 caacggcgat acggtcgaga tgttggctct tgtcagaaag tttattgata acttcttcag
    19441 cattttggta caatttcacg aattgtacgt tttcaagagt tgctttaaat tcgcggtttc
    19501 ctttaacaat ttcaacacct tcgattggtg gcaagattgg tgtagaacct gtagcgaaaa
    19561 tcaatttctc gtatgattct ttgtgttctt ttccttcaac ttctgctgta accactttgt
    19621 tatcatagtc gattgaaaga actggtgaat tcatataaac cttagcacct ttagcttcta
    19681 atttgtcttt atcagaatag aacaagcctt cagcaccgtc aatttgttca ccaatccaaa
    19741 gagccattcc acatcctagg aaagagatgt tagagttttg gtcaaataca acaatttcgt
    19801 tctcatttcc aaaattatcc aacatggtat tgatacatgc tgtaccagcg tggttagcac
    19861 cgactacaac gattttactc ataaaaaatt tcctgccttc attctaaatt tactttacta
    19921 gtataccatt taccgttagc gtttacaagg gggggactat tatttcagca ttttttatca
    19981 aaaataattc cccatttatc ctgttttcaa gcctcatgaa ttttatgagc atatttcttg
    20041 actttttcca aattttgttt gtgaaaattc taactttatg gtattataat aacatgaaaa
    20101 taaaatgtaa gggcttcaat ttagatgatt atttgatatt gcaacttatt ttctataaga
    20161 gaaaggagtt ggtagcttga ctcttcgttc acattctgaa cggctaatgg ggactactat
    20221 cactatttca ttagtggatg agcaagccga tatctttctc caaaaatcct ttgacttgct
    20281 caaagagctt gaataccgct tcaacgccaa tagtcaagaa tctgagttga tggaaatcaa
    20341 ttatcaagca ggaatatccc ccgtcacagt tcatccagac ctgtttgagc tgatttcact
    20401 tgggttagag catagcctag cgctctctag ccacctcaat atcagcattg gtcccttaat
    20461 tcaaacctgg cgtatcggtt tttcagatgc caaggtcgcc cagcctcaag aaattgaatc
    20521 ggtgctgcct ctaatcaatc ctcatggtat cgagttagat tcttctactt ctactgtgtt
    20581 tttaaaacag aaaggaatga agatcgatct aggttgttta gccaaaggat acagtgcgga
    20641 taaggttgcc caatttctta ggaaagaggg ggtgacttct gccttgatca atctgggagg
    20701 gaatatcctg accattggaa aaaatcaggc aagaggggat aacccatggc aaatcgggat
    20761 tcaagaccca gccaatccta ggggaaatca tttaatgacc atccctgttg tcaataaatc
    20821 tgtcgtgact tcaggcattt atgaacgtca cctgaccgtc gatggacaag attaccatca
    20881 catttttgac agtcaaacag gatatcctgt tgaaacggaa ctagcgagtc taacaatcat
    20941 ctctgataaa tcagtcgatg gcgaaatctg gacgactcga ctctttggag aaagacctgc
    21001 ttctatcctc tggcaagtcg aaagtttgga gggcatcgaa gtcatcctca tcgataaaga
    21061 aggccaccta agctgttctt caggaattcc tactctatag agacaactgt ttcaatggaa
    21121 ttttaaaatt gtattatgta aaaagagaaa ggaaatatca tgttaaaact tattgctatt
    21181 gttggaacaa attcaaaacg ttctacaaac cgtcaattgc ttcaatacat gcaaaaacac
    21241 tttgctgaca aagctgaaat tgaacttgtt gaaatcaaga ccattcctgt cttcaacaaa
    21301 ccagctgaca agcaagtacc tgctgaaata ttggaaattg ctgctaaaat cgaagaggca
    21361 gatggcgtta ttatcggtac tcctgagtat gatcactcta ttccagctgt tttgatgagc
    21421 gctcttgctt ggttgtctta tggtatttac ccacttttga acaaaccaat catgattaca
    21481 ggtgcttctt acggtacgct tggttcatct cgtgcccaat tgcaacttcg tcaaatcttg
    21541 aatgctcctg aaatcaaggc aaatgttctt ccagatgaat tcttgctctc acactctctt
    21601 caagcattta acccaagtgg cgacttggtt gaccttgatg ttatcaagaa attggatgcc
    21661 atctttgatg acttccgtat cttcgtgaag attactgaga aattgcgcaa tgcgcaagaa
    21721 ttgcttcgca aagatgctga agactttgac tgggaaaatt tgtaagatag gagaccgaaa
    21781 gaatgaaatt tgttggactt gttggatcaa actacgatca atcatataac cgcaaactct
    21841 tggaatttat ccgtcgcaat ttcaaattca aatttgaatt agaagtactt gaaatcgacg
    21901 aagttccaat gtttaaccaa gacgaaaaat gggacgaaag cttccaattg cgtttcttgt
    21961 ataacaagat tacacgtgct gatggtgtca ttatcgccac tcctgagcac aaccacacta
    22021 tctcagcttc actcaaatct gtacttgaat ggctttcata cgaagttcat ccatttgaaa
    22081 acaagcctgt tatgattgtg ggtgcatcat actatgacca aggaacatca cgtgctcaag
    22141 ttcaccttcg taaaatcctt gatgctccag gtgttaatgc ctacacgctt ccaggtaacg
    22201 aattccttct tggtaaagct aaggaagctt ttgataacaa cggaaatatc accaacgaag
    22261 gcactgttaa attccttgaa acttgcttag ataactttgt taaatacgta ggagtcgttt
    22321 cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga aaaccaatcg
    22381 ctacaaccat cacagaagtt gaccctgacg atccagaatg ggtagaaaaa gttgcagcaa
    22441 tcactggtgc tgtttctggt gatacctatg tcaaattgga ccacggtatc ctaacagtta
    22501 accaaattga tatgttcttg aaagctatgc catttgaatt gacatacgct gacgacaaca
    22561 accaattcct ctactacaac aacgctcacc aagatccaga cactatgttc gctaaacgtg
    22621 taccacctca atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta
    22681 tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta
    22741 cgatcgttcc aggttctcct gcaggtgtta tcaacactca caactaccaa gcaatgtact
    22801 atcctgatgg atcatacgct ggtatcaatg aaatcgtctt taacttccaa ccatggcttg
    22861 actggtacct aaaagagact ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg
    22921 ctgccggagg tcatggagac gccgatgcta cttctggcgc ttctgattca ggggatgctg
    22981 gaggccacgg tggtgacgca gacgctacag ccggtgcaag tta
//
LOCUS       DAVBCR010000026        22485 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCR010000026 DAVBCR010000000
VERSION     DAVBCR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.026"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            145..846
                     /locus_tag="VQ508_000985"
     CDS             145..846
                     /locus_tag="VQ508_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HET2490000.1"
                     /translation="MYSGFTHYKYYRAEIIRKVQEPEFWKEVNLSDLEKIRLAIRDLL
                     QFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKL
                     YHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSK
                     FLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNI
                     VQIIELINNRAGEAA"
     gene            complement(1246..2592)
                     /locus_tag="VQ508_000986"
                     /pseudo
     CDS             complement(1246..2592)
                     /locus_tag="VQ508_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
     gene            2654..2848
                     /locus_tag="VQ508_000987"
     CDS             2654..2848
                     /locus_tag="VQ508_000987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490001.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            2826..3026
                     /locus_tag="VQ508_000988"
     CDS             2826..3026
                     /locus_tag="VQ508_000988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490002.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(3106..3551)
                     /locus_tag="VQ508_000989"
                     /pseudo
     CDS             complement(3106..3551)
                     /locus_tag="VQ508_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3559..3702
                     /locus_tag="VQ508_000990"
     CDS             3559..3702
                     /locus_tag="VQ508_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490003.1"
                     /translation="MAERFWENLSIILADRNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            3795..4382
                     /locus_tag="VQ508_000991"
     CDS             3795..4382
                     /locus_tag="VQ508_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490004.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            4782..5591
                     /locus_tag="VQ508_000992"
     CDS             4782..5591
                     /locus_tag="VQ508_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2490005.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            5596..6297
                     /locus_tag="VQ508_000993"
     CDS             5596..6297
                     /locus_tag="VQ508_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2490006.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            6322..7239
                     /locus_tag="VQ508_000994"
     CDS             6322..7239
                     /locus_tag="VQ508_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2490007.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            7267..8808
                     /locus_tag="VQ508_000995"
     CDS             7267..8808
                     /locus_tag="VQ508_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2490008.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            8827..9285
                     /locus_tag="VQ508_000996"
     CDS             8827..9285
                     /locus_tag="VQ508_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2490009.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            9287..11509
                     /locus_tag="VQ508_000997"
     CDS             9287..11509
                     /locus_tag="VQ508_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2490010.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            11549..12658
                     /locus_tag="VQ508_000998"
     CDS             11549..12658
                     /locus_tag="VQ508_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2490011.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            12815..13705
                     /locus_tag="VQ508_000999"
     CDS             12815..13705
                     /locus_tag="VQ508_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2490012.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            13730..14053
                     /locus_tag="VQ508_001000"
     CDS             13730..14053
                     /locus_tag="VQ508_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490013.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            14043..16034
                     /locus_tag="VQ508_001001"
     CDS             14043..16034
                     /locus_tag="VQ508_001001"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2490014.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSETKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMIILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFVWCVILLGLLF
                     IAVGMLMPDMRLLFVLGKWVSIFNAVGILVVSIIQAKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            16050..16526
                     /locus_tag="VQ508_001002"
     CDS             16050..16526
                     /locus_tag="VQ508_001002"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2490015.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            16560..17141
                     /locus_tag="VQ508_001003"
     CDS             16560..17141
                     /locus_tag="VQ508_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490016.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            17206..18213
                     /locus_tag="VQ508_001004"
     CDS             17206..18213
                     /locus_tag="VQ508_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2490017.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            18203..18523
                     /locus_tag="VQ508_001005"
     CDS             18203..18523
                     /locus_tag="VQ508_001005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2490018.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            18586..20361
                     /locus_tag="VQ508_001006"
     CDS             18586..20361
                     /locus_tag="VQ508_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2490019.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            20362..21747
                     /locus_tag="VQ508_001007"
     CDS             20362..21747
                     /locus_tag="VQ508_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2490020.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            21770..22381
                     /locus_tag="VQ508_001008"
     CDS             21770..22381
                     /locus_tag="VQ508_001008"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2490021.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         7474 a         3321 c         4485 g         7205 t
ORIGIN      
        1 atttggatat ttgtgtcttt gatatcgagt agttttgtga taaaatgtaa ttgttccata
       61 tgaatctttc taatgagttg tttggtcgct tttcattata gatcttatgg gacttttttt
      121 ctacaataaa ataggctcca taatatctat agtggattta cccactacaa atattataga
      181 gccgaaatta tcaggaaagt acaggagcct gaattttgga aagaagttaa cttgtctgat
      241 ttggaaaaaa ttcgtcttgc tattcgagat ttattacagt ttttggataa aacagaccgt
      301 aaaccctact atgttaactt tgaagatcgt atactctcca ctgttcacga gaccacagca
      361 tttttgcagg tcaacgatct tcggtcttac aatgaaaaag ttgagcatta tttgaaaact
      421 catctggatg aggagtccat ttctaagcta taccataata aaaagttgac atctgatgat
      481 atgcttgcac ttgaaaaatt gctttgggaa aaattaggta gtaaagcaga ctaccaaagt
      541 cattatgaaa ataaggcaat tccgagattg gttcgtgaga ttattggctt agatagagag
      601 tctgccaatc gtattttctc taaatttttg tcggatgaga atcttaatgc caggcagatt
      661 tcatttgtaa aattgattgt agactacatt gtagaaaatg gttttttaga gacgaaagtg
      721 ttaacgcaag agccgtttaa atcttatggt tctgttcaac tactcttcca acaccaacta
      781 ccagtacttc gtaatattgt tcaaatcatt gaacttatca ataatcgagc tggagaagcg
      841 gcttaaattc taaagtgatt gccatgctga gactcattta aaattaaaaa gagtagaaat
      901 ttatgctata tatgagaagt tttattagga agaatgtcat cgttttccta gaatacagta
      961 tcagttgtta agtggttgat aaatttcaaa gtagatactt gtaccacgat gtttgttgat
     1021 cgagttatta acaaaagagc tactttgatt ttaacccaaa tcattcatac ctctctcaac
     1081 tagatgtaac ttacaaaacc cctgacctca tgagccactt tcttcctcct catgaggtca
     1141 gttttacttt ctgctgttcc agtatcgttt ttcctcgcta gatttcctca aaagggcaga
     1201 ctcctccctt ggtgcgtcac acgatttttt catctcgact gttctttaat gcatcattaa
     1261 cgacgctttt cttccaggtg gttcataagg aacaggaaga ttcaggttga cttttctaat
     1321 cctagaataa agtgctgaaa acaattcgga ataggcatag agactagaca atttgaggag
     1381 ctgcttgcgt cctgttcgaa cacattttcc taccacgtga agaaaaagat ggcggaagcg
     1441 tttgattgtt aaagtttgga agtcacctcc agctagatgt ttgagaaaaa gatagagatt
     1501 gtaggcgata cagctcatca tcatacgaac ttcgtttttg attaaggttg aactatccgt
     1561 tttatcgcca aaaaatccct ccttcatctc cttgatgaaa ttctcggctt gaccacgtcc
     1621 acgataaagc tgaaactggt cttggcttgt tccactcgtc atatttgtaa cgagagaaat
     1681 aacatcgtag aacaagtttc cttcttttcg ttcagagaac tggcagacac gacgcttgtg
     1741 cgaccaagat cctgcttgat agagagtttc tgagtaggcg gagtggggca agatggttaa
     1801 gtcctcatcc tgtgggcaag ggagggaaag gtctccaaga cggctcagaa cagtattttt
     1861 cttgagtttt atgagttagt attgccctgt tttttcaatt aaatcgtata atttgggggt
     1921 cgcaaagcca ctatccattc gaaagagaag ttgattaaac cgttctaaaa caggtgtgat
     1981 aaagctgtct gcctcttcag aacaataacg attaccagga cgaagctggg cattgaaaca
     2041 ataacctgtc ttcccctcga aagcataaag aggatgatag ccatgagcac gatagtgggc
     2101 gttataagca acaccttctt gcttgccata agttgtgaaa tgggtagaat cgatatctac
     2161 aatgagttgg tttagctggt gaaactgtaa aaagaattcg accaattcaa ggttgaggca
     2221 tcgcaaacta tggactgttt cctcgtcagt tctggaaaga aaacgggata aggttggctg
     2281 tgaagcaagc tgccctcctt ccaacaattt tggaaagtag gcatcagctg acaattcttt
     2341 acaagcatag tccgttccat agcctgttaa cagttgaaag aggaactgga caaggatatc
     2401 tgaatccgaa taacgacagt agcggcgttg gtcattcgtt actaaatact tagaaatccg
     2461 ctcttttagt ttcaactggg aaaaaagttc ctgaaaaaag ataagaccac catactgggt
     2521 taaatgacct ccatcgaaag atagttggta aaaagacttg ttttggaagt gatgatttgg
     2581 taaactgttc atgtgagttt cctttctttt tgtgtttttt tctacactta taccataaag
     2641 gggaaattct tttttgtcta gtaaaaaaca cccattgggt gaaaaaagaa accatacagg
     2701 atctaagcta aggcaaggat tctggatggt ttttagatta ggggtgaata attggggttt
     2761 taaagaaata gaaaacaaaa agccgagcaa gaattcaatt gcaggagaaa atgaaataat
     2821 actcaatgaa aatcaaagag caaactagga aactagctgc aggctgctca aaacactgtt
     2881 ttgaggttgt agataagact gacgaagtca gctcaaaaca tggttttgag gttgtagatg
     2941 aaactgacga agtcagtaac catacctacg gcaaggcgac gttgacgcgg attgaagaga
     3001 ttttcgaaga gtataaatct tcctaggata aagcaaagag cctgggacaa aatagttctc
     3061 gaccacaaaa aagctagaga tttccaattg tggaacttta gctttttaat tttgagtcgt
     3121 tctcttattg tattttagga ggttattggc cataagtacc aatcccatgt caattctcac
     3181 ttgacgcttg cccctcagat tacatctctt gtaacccaaa caagccttta tctgcccaaa
     3241 gacaggtgac attctttagc tttcaagttt tgatagcctt cgttcatata cagtcccttt
     3301 tgaggggctg attcaggttc gtcggcgtag taaaccttga tttcttgttt aaagtctgtc
     3361 tgtgttttct gatgtttggt atggtgaaaa cgataatacc agccatcagg atgtgtatag
     3421 ctatcctcct tgtcattata gtaccaattc gctaagtttc tagctgactg tttatagcct
     3481 ctcttctgtt ccttatcaaa catggcatat ttaatcagat ggtttacctc cttttcatct
     3541 aactaaagga gaagcaatat ggctgaacga ttttgggaga acttgtccat tattttggct
     3601 gacagaaata tcagctggat tgagttaacc agaaaaatgt ttgcgggaga gtttcactat
     3661 ccaagtgaat tgaatcgttt gtatcaaaag attcgccact agaagatgga acaacgaatg
     3721 cctcaaagtc catgggtaga aaggattgtg caggtattag atttagatta tgaagattta
     3781 tttcggaggt aattatgaaa agaataattc cagtttatat attccaacaa gtaaatgtcc
     3841 tattggtatc tctatactta ttgaaatttc tttgtagcga tgagttaact atactacaga
     3901 ttctctatgg agcgtcactc atttcttttt tatggatgta tggtcaacga aaacaagtga
     3961 tcaaggttaa tatgaaaact aggatacaat ggcttggtat tggattcgtt agcctactga
     4021 ttataagtct atgttttagt ctaatccatg cgcaagaaag cacgaatcaa gcaaacttaa
     4081 ttggacttca acatcaaatt ccttggtttt catttttatt gtttttaatc aatgcgagta
     4141 tggttgaaga atttctgtat agagaaattt tatggaactt ggttagaaaa ttagatattc
     4201 gagttgcttt gacaagtgtt ttatttgcct tagcacatca tccaggaacc attatagctt
     4261 ggtgtttgta tgtatcgctg gggatgtttt tagggcttgt acgctacaaa ttggacttat
     4321 ggggcagtat ggggctacat ttggtgtgga acctactagt ttatagtttg ctgctttttt
     4381 aaaataagtg atctgattta tctcataaat cagtagtttt tataggatta tttttaattc
     4441 ggaaacaaaa atgtattaac agcataaaat aaagaaacta ggaacatttt ttttattttt
     4501 tagaaataaa atgttgtata gaaattccga aagaaataat aaaatcaaaa taaaaattaa
     4561 taaaataggt atatttgaac atattgacgt cgacaaatgt tcttgcaaga cataaaatac
     4621 aaataaaaag aataaaaaat ttttatattt ttatatttgt tgttctgtta taaaatgaaa
     4681 gaaattagaa actagtttct aaatcatgaa aaatattgaa aaaaaaaaaa ataagtgtta
     4741 aaataaagaa tgtaaacgtt tacttcaata aaggagctca tatgttactg caaaaagaac
     4801 taattccaat gatagaagct aacttaccaa atatggcata tgctgaaaaa gacattgcta
     4861 aattcttctt aaaacagcaa cctctgaata attattcatg taaggcattg tgcgaatgcc
     4921 ttaatgtatc caaagcaaca ttgactcgat ttgcgaaaaa atgtggtttt aaaggtttta
     4981 gacaattcat tttcaaatac caagagatga ttcgtgagaa agaaaagtta gcattgtata
     5041 cagaggctac agaaaaagtt ttatccgact atgaagaaat gttgagaaaa acttacacgg
     5101 ttcttgatga agttcaactt gagcgtattg ctgagatgat agaaactgct gagcgtgtat
     5161 atctttatgg taaagtaagt tctgttcttg ctttacaaga aatgaagatg agatttatgc
     5221 gtctcggagt cattggtgaa gtattatcag acgaggatat gattttgtgg aatagcttat
     5281 tacttaatga aaattgcctt gtcattggag catccgtttc aggtcaaact gatattgtac
     5341 tagaaggtct tcaaaaagct gcggataaag gcgctaaaac agttttaatg actacaagaa
     5401 aatttgacga agaagattgt ttctttgatg aactattgtt attagcttcg accaatcatc
     5461 tctcgtatgg caatcgcata tcacctcagt ttccaatact tttaattaca gactgcttat
     5521 tctctcatta tctggaaagt ccagagagac aatattatta caatcaaact attatccata
     5581 aggaggaata ataaaatgag tcaaatttgg actaaagaaa aatttataag tcaagtccat
     5641 ggtggagtca ttgtttcttg tcaagcttta cccggtgaac ccctttataa tgaagaattt
     5701 agcttgatgc cttttatggc taaagcagct ttagaggcag gagcagtggg cattcgcgca
     5761 aattctgtgc gtgatattaa agcaattcag aaagtagtag atttaccaat aattggaatt
     5821 atcaaaagag attatccacc tcaagaacca tatattactg ctacgatgaa agaagtagat
     5881 gaacttgtag aatgcggaac aacagtcatt gcatttgatg caactttaag accaagatat
     5941 gatggcttag ttatcaatga atttatcaaa aaaataaaag aaaaatatcc taatcaattg
     6001 ttgatggcgg atgtaagtaa ttttgatgaa ggtctctatg catttaaatc aggcgttgat
     6061 tttgttggta caacattatc agggtacaca agtacaagtg tacaatcaga tgagcctgat
     6121 tttgaactaa tgaaaaaatt ggctgatttt aatattccgg taattgccga aggaaaaatt
     6181 cattatccag aacaattaaa aaaagcttat agtttaggtg ttaccagtgt tgtcattggt
     6241 ggagcgatta cacgtccaaa agaaattgct cagcgattta ttaatgtcat caaataaaaa
     6301 acaatataaa ggagaaatct gatgaaagat ttaactaaat acaaaggcgt tattcctgca
     6361 ttttatgctt gctatgatga aaatggtgaa attagccaag aacgtgtaaa atctctggtg
     6421 caatatttca ttgacaaagg tgtaaaaggt atctatgtaa atggttcttc aggtgaatgt
     6481 atttaccaaa gtgtagaaga tcgtaaacaa attattgaag ctgttatgga agttgctaaa
     6541 ggtaaattaa cagttatcaa ccatgttgca tgtaataaca cgaaagatag tattgaattg
     6601 gcaaaacatt cagaaagtgt tggagtcgat gctattgctg ctatcccacc tatttacttc
     6661 aaattgccag aatattcaat tgcagcatat tggaatgcaa tgagtgaagc tgcgccaaat
     6721 acagatttta tcatttataa tattccacaa ttggcagggg ttgcgttgac tggtagtttg
     6781 tatgcaacaa tgcgtcaaaa tcctcgtgtg attggagtta aaaattcttc tatgcctgta
     6841 caagatattc aaatgtttgt agctgcaggt ggagaagatt acattgtatt caatggtcca
     6901 gatgaacaat atcttggtgg tcgcttgatg ggagcagaag ctggtattgg tggtacttat
     6961 ggcgttatgc cagatttgtt cttgaaattg gaaagtttga ttcaagaacg agatttagat
     7021 actgctaaaa aacttcaata tgctatcaat gaagttatct ataagatgat atcaggtaag
     7081 gcaaatatgt atgctgtagc aaaagaggtt ttgcgtctaa atgaaaaact tgatttaggt
     7141 tctgttcgtc aacccttaga agcattggca gaaggtgact tggaagttgc aaaacaagca
     7201 gcagaactta ttcaacaagc acgaaaagaa tttttataat aaataaaatc aatatggaga
     7261 tatgttatgg gcacaacagg atttacaata attgacttaa ttatcttgat tgtttattta
     7321 cttgcggtgt tggttgcagg tatctatttc tctaaaaaag agatgaaagg aaaagagttc
     7381 ttcaaaggag atggttcggt tccttggtat gttacttcgg tatccatttt tgccacaatg
     7441 ctcagtccga tttccttctt gggactcgct ggtaactctt atgcaggtag ctggatttta
     7501 tggtttgctc aattagggat ggtagtagct attccactga caattcgttt tatcttacct
     7561 atctttgcac ggatagacat cgatacggca tatgattact tggataaacg ttttaattct
     7621 aaagcacttc gtattatttc agcactcttg tttattattt atcaattggg acgtatgtct
     7681 atcattatgt acctcccatc agctggttta tcagtattga caggaattga catcaatatt
     7741 ttgattattt tgatgggtgc agttgcaatt gtttattctt acactggtgg tctaaaatcc
     7801 gtattatgga cagactttat tcaaggtgtg attctgatta gtggtgtcgt tttagcttta
     7861 tttgtactga tggctaatat taaaggtggc tttggtgcag tagcagaaac attagcaaac
     7921 gggaaattcc ttgctgcaaa tgaaaaactt ttcgatccta acttgctttc aaactccatc
     7981 tttttaattg tgatgggttc aggctttaca atcttgtctt cctatgcttc atctcaagat
     8041 ttggttcaac gttttactac aacacaaaat attaagaaac ttaataagat gttgttcaca
     8101 aacggtgtct tgtcacttgc aactgcaaca gtcttttact tgattggtac aggcttgtac
     8161 gtattctatc aagtacaaaa tgcaggtagt gcagctagca atatccctca agaccaaatc
     8221 tttatgtact ttattgcata ccagttacca gtaggtatca caggtttgat tttggcagcg
     8281 atttatgcag catctcaatc aactatttca acaggtttga actctgttgc aacttcatgg
     8341 acattggata ttcaagatgt catttctaaa aatatgtcag acgatcgtcg tacgaaaatt
     8401 gcacaattcg tatctctagc agtaggttta ttctcaattg gtgtttccat tgtcatggct
     8461 cactcagata ttaaatctgc atacgaatgg ttcaatagct tcatgggact tgtacttggt
     8521 ctacttggtg gtgtatttat tcttggattt gtttctaaaa aagcaaataa acaaggtgct
     8581 tatgcagcgc tgattgtatc aactatcgtc atggtattta ttaaatactt ccttcctcca
     8641 acagctgtga gctactgggc atattcattg atttcaatct ctgtatcagt agtttcaggt
     8701 tatattgtat ctgttcttac tggaaataaa gtatctgcac ctaaatatac aacgattcat
     8761 gatattccag aaattaaagc ggattcaagt tgggaagttc gtcactaata gaaaagagaa
     8821 aataatatga ttatttctga acaatctgat tttagacgat atgcttctat caataaacat
     8881 ttttcaaaag tctgtgattt tttagaaaat acaaatttga cagatttagt tgatggaaaa
     8941 gttgatattg acggggaaaa tgtttttgca aattgtatga tctatctagc tgatggagtc
     9001 ccaggagata tttttgaaac tcataagaaa tatttagata ttcatattgt tgttgaaaat
     9061 actgaaaaaa tggcagtaac ttccccagtt cgtgcccagt cacgtgtacc ttttagcgaa
     9121 gaaaaggata ttgcttttta cgatagcaaa gactatcaaa ttgttgaatt gcttccaggg
     9181 aatatgttgg tgacttttga agaagatctt caccaaccaa agattcattg caatgatgag
     9241 cctgttaaaa aacttgttat taaagtttta aacgaggaaa aataaaatga aaaaaaatat
     9301 taaacaatac gtaaccttag gtactgcagt ggtattatca gcatttgttg ctaactcagt
     9361 tgcagctcag gagactgaaa cttctgaagt atcaacacca gagttggtgc aacctgttgc
     9421 accaacgact ccgatttcgg aagtacaacc taaatcgggt aactcttcgg aagttactgt
     9481 acaacctaga acagtcgaaa ctactgttaa ggatccatct tctgcaacgg aagaaactcc
     9541 tgtcttagaa aaaaataatg ttactttaac agggggcgga gaaaatgtta ctaatgagtt
     9601 aaaggataaa tttactagcg gtgactttac tgtagtgatt aagtacaatc agtctagtga
     9661 gaaaggctta caagctctgt ttggaatatc taattctaaa cccggtcaac aaaatagtta
     9721 tgtagatgtg ttccttagag acaatggtga attgggaatg gaagcgcgtg atacttcttc
     9781 caataaaaat cacctagtat ccagacctgc ttcagtttgg ggtaagtaca aacaagaagc
     9841 tgtgactaac actgttgcag tagtagcaga ttcagtcaaa aaaacatatt ctttatatgc
     9901 aaatggtaca aaagtagtag aaaagaaagt ggataacttc ttaaacatca aggatattaa
     9961 aggtattgat tactatatgc ttgggggagt gaaacgtgca ggaaaaacgg cgtttggttt
    10021 taacggaaca ctagaaaata tcaaattctt taatagtgca ttggatgaag aaactgttaa
    10081 aaagatgaca acaaacgctg ttactggaca tttaatttat acggctaatg atacaacagg
    10141 ttctaactat ttccgtattc cagttctgta tacttttagc aatggtcggg tattttcaag
    10201 cattgacgct cgttacggcg gaactcatga ttttttgaat aaaattaata ttgctacaag
    10261 ttatagtgat gataatggta agacatggac taaaccaaaa ttaacattgg cattcgatga
    10321 ttttgcgcca gtaccattag aatggcctcg tgatgttggt ggacgtgact tacaaatcag
    10381 cggtggtgca acctatattg actctgttat tgttgaaaaa aataacaaac aagtactcat
    10441 gtttgcggat gtgatgcctg ctggagtaag ttttagagaa gctactagaa aagattcagg
    10501 ttataaacaa attgatggta attattacct taaattaaag aaacaaggtg atactgatta
    10561 caattatact attcgtgaga atggtactgt atacgacgat cgtaccaaca gaccaactga
    10621 attttcagta gataaaaatt tcggtattaa acaaaatggt aattatttga cagtagagca
    10681 atattcggtt tcatttaaaa ataataaaaa gactgaatat cgtaatggga ctcacgtcca
    10741 tatgaatatc ttctacaaag atgcattgtt caaagtagtg ccaacgaact atattgcata
    10801 tatttctagc aatgatcatg gcgaatcttg gtctgcacca actttattac ctcctataat
    10861 gggacttaat cggaatgcgc cttatttggg tcctggacgt ggaatcattg aaagctcaac
    10921 tggacgtatt cttattccgt cttacactgg taaagagtct gcattcattt atagtgacga
    10981 taatggagca tcttggaaag ttaaagttgt gccacttcct tctagttggt cagcagaagc
    11041 acaatttgta gaattgagtc caggagtaat tcaagcatac atgcgtacaa ataatggtaa
    11101 aattgcatat ttaacaagta cagacgcagg tactacttgg agtgcaccgg aatatttgaa
    11161 atttgtttca aatccaagtt atggaacaca attatcaatc atcaattata gtcaactgat
    11221 tgatggtaaa aaggctgtca ttttaagtac tccaaactcc acaaatggtc gtaaacacgg
    11281 acaaatttgg attggtctaa ttaatgatga taatacaatt gattggcgtt atcatcacga
    11341 cgttgattat agtaactatg gatactcata ttcaacattg acagagttac caaatcatga
    11401 aattggattg atgtttgaaa aatttgattc atggtctcgt aatgaacttc atatgaaaaa
    11461 tgttgtacca tatataacat ttaagattga agatctgaaa aagaattaaa gctgaaattt
    11521 gaaaatatat aaaaagagga taaaaattat ggtaaattac ggtattgttg gagctggata
    11581 ttttggagct gatttagctc gctcaatgaa caaaattgaa gatgcaaaag tggttgcggt
    11641 atttgaccca aatcatggag aagaagttgc tcaagagttg ggatcagatg tttgtgcaag
    11701 tttagatgaa cttgtagcac gtgaagatat tgattgtgtg atcgtagctt cacctagcta
    11761 ccttcaccgt gaaccagttg tgaaagctgc tcaacatggc aaacacgtat tttgtgaaaa
    11821 gccaattgca ttgtcttatg aagattgtaa agccatggtt gacgcatgta aagaaaataa
    11881 tgttatcttt atggctggtc acatcatgaa cttctttaac ggtgtacacc atgctaaaga
    11941 attgattact caaggtaaaa tcggtaaagt tctttattgc catgccgctc gtacaggttg
    12001 ggaagaacaa caaccaactg tatcatggaa gaaacttcgt tctcaatctg gaggacattt
    12061 gtaccatcat attcatgaat tagattgcat tcagtttatc atgggaggac ttcctgaaaa
    12121 agcgacaatg gtaggaggca atgtatatca taaaggtgaa aactttggtg atgaagatga
    12181 tatgctcatt gtaaacttag aatactctga tgatcgttat gctgttttgg aatatggtaa
    12241 tgctttccgt tggggtgaac actacgtctt gattcaagga actgaaggag ctatcaaact
    12301 tgatttgttc aatactggag gtactcttcg tgttaaaggt gaaggagaat cacacttctt
    12361 agttcatgaa actcaagagg aagatgatga ccgtacagct atctataccg gtcgtggtat
    12421 ggatggagca attgcgtacg gtaaaccagg agtacgttgc ccattatggt tgcaaacatg
    12481 tattgataaa gaaatggaat atctacatga catcattaaa ggtggagaaa ttacagaaga
    12541 atatgaaaaa cttctcaatg gtgtagctgc tttagaatca atcgctaccg ctgatgcatg
    12601 tactttatca gttaaagaag atcgaaaagt aagtctttca gaaatcacaa atgcttaact
    12661 tttgtaaaac agaatagtaa attcttatca ttatataatt tctaaagttc tgtgatacaa
    12721 ctcattgaat aaagaaatag agatgggact gggataatgc ccagtcccat tttttatcaa
    12781 aaagtaatga gatcaaaaat gtgggagtga tgaaatgaag attataggga tcgatattgg
    12841 cggaacaaca attaaagcag atttatacaa tgagtttgga acgagtttaa atcagttcaa
    12901 agagatagaa acaattattg actatgattt gggaacgaac cagatattaa atcaggtctg
    12961 tgatttaatt ggtgagtata ttttaaatca ttcaattgat ggtgttggga tttccactgc
    13021 tggagttgtt aatgctaata caggagagat catctataca ggctatacaa taccagggta
    13081 tatcggagta aactttactt tcgaaataga aaaacgtttt gggttgtcta cttttgttga
    13141 aaatgatgtt aattgtgctg cattaggtga attgtggaag ggacaagcca aagataagaa
    13201 aaatgtagta atggttacta ttggaacagg tataggaggc agtattattg tcaatggaca
    13261 aattgttaac ggatttaact atactgctgg tgaagtaggc tatattcctg taggtaattc
    13321 ggattggcaa agcaaagcct caacaaccgc attgattcat ttatatcaaa agaaaagctt
    13381 gaaaactaat caaactgggc gtactttctt tactgattta agagctggag ataaagtcgc
    13441 taaagaaact tttgaaattt ttgtagaaaa tctaacaaaa ggtttattaa cgattactta
    13501 tctacttaat ccagaaattc tcatattagg aggtgggatc ctggctaaaa aggatatttt
    13561 gttacctgaa attcaaagtt ctttagctaa aaatgcaatg gataataggt ttttgcctaa
    13621 aaatcttgtg gcagctacat taggaaatga agctggtcgt ataggagctg taaaaaattt
    13681 cttagataga atttctaata aatagtatga aagataagga ggtgtcacaa tgactaactc
    13741 tgtattttcg acaatgcaag atattgagaa tgttgcaacc gatattataa aatcatatga
    13801 taatgagatt tatacttata aagctgtttc ccaagaagaa ttggaaaaac tagaaaaaag
    13861 ttatgatgaa aaaagtcacg aagaattagt ttcaatagaa agcaatttag aaatgaaaca
    13921 acagaacctt attgatgagg ttaataaaac aatcaaggaa aattatgaaa agattcagta
    13981 tatttcatca agtaggagag gagaatttgt agaaaaaatt attggtaggg tggtagaaaa
    14041 atatggctat tagtcagatg aaaagaattt ctctactatt ttctaaaagt agtcttgatg
    14101 atgttttaaa aactattcaa gaactagagt cagtgcagtt ccgtgattta aaggttcagg
    14161 ataactggtc agaagctcta gaaaaagatg aggttgtatt tccaactatt caaatttctc
    14221 atacttctaa ttccaatcat ggggttattg aaggaaatga tgccttgatt tatttgatga
    14281 atcaacaaca atatttagaa gcaactgtag agaaattaca agaataccta ccgaaagaaa
    14341 acacgtttaa attattacag caacctccga taacgacctc ttatgaagaa ttagaaaaat
    14401 ttggtaaagc taatgttgct gagggtgttc ttaaaaaagt gaatcgtcaa ataaacagag
    14461 ttcatgaatt agaaagacac attcaaagta ataatgagga aatagagaga ttaataaaat
    14521 gggaaaaatt agaaattatt cctgcgcatt tagaacaatt ttttttctgt aaaggaaaag
    14581 tcggaacaat tccaaggact gtagataatc gcttatacaa tagtttttta gaaaacaata
    14641 ttgaagttca agaaatattt tctaatgata gagagtacgg tgttgttgtt ttctatcagt
    14701 ccagttgctc tattgatttt gatgaatact tatttgaacc atttgattat tctagaaagg
    14761 aattaccgaa gcagcgagta gtagatttgg atcaagaaaa catgcagtta ataactgaaa
    14821 aagagaatat tatcgcatcg ttgcaagatt caaagaataa tttgatagat ttacaatggc
    14881 aaattgacta tattttatct atctatgctc gtcaaatctc taagaataac cttttgagca
    14941 ctccgcatct agttgcacta gaaggatgga tagaagaaac tcgtatttta tattttataa
    15001 aagttatgga tgagcgtttt ggacattcta tttatattta tgaatcggaa acattgtcgg
    15061 ataatcaaga tgaaatacct atcaaattaa cgaatcattc tttaattgaa ccatttgaat
    15121 tattgacaga aatgtatgct ctgcccaaat attatgagaa agatcctaca cctgtattag
    15181 caccatttta ctttacattt tttggaatga tggttgctga tttaggctat ggtttactat
    15241 tgtttttagg aacaatgtta gcattaaaaa tttttcatct accttcagaa actaagagat
    15301 ttttaaaatt ctttaatata ttaggggtag ccgttgcaat ttggggtgga atctatggct
    15361 cattttttgg atatgagttg ccatttcatc tgatatctac aacctctgat gtcatgatta
    15421 tattagtagt gtcagttgtg tttgggttta ttacagtatt tgcaggtttg ttagcttcag
    15481 gactacaaaa agtaagaatg aagaaatatg cagaagcata taattcagga tttgtgtggt
    15541 gtgttattct gcttggcttg ttatttattg ctgtcggaat gttgatgcct gatatgagac
    15601 tgttatttgt attagggaaa tgggtatcta tttttaatgc tgtgggaatt ttggttgttt
    15661 ctattattca agccaaaagc ttgtcaggta ttggagcagg attgtttaat ctatataaca
    15721 tttcatctta tataggtgat ttagttagtt tcactcgatt gatggcatta ggattatctg
    15781 gagcaagtat agcatcagct ttcaatttaa ttgttggttt gtttccggga acattggcta
    15841 aactaacaat tggattagta ttattcattc ttttacatgc gatcaatatt tttctatcgt
    15901 tactatcagg atatgttcat ggagcacgtc tgatatttgt tgaatttttt ggtaagtttt
    15961 atgagggtgg aggaaaacca tttcaacctt tgaaggcttc tgagaaatat attaaggtta
    16021 ttacaaagaa ttaatggagg atatatataa tggaacattt agcaacttat ttttcaacct
    16081 atggaggagc tttcttcgct gcattgggaa ttgtattggc ggttggatta agtggtatgg
    16141 ggtctgctta tggagttggt aaggctgggc aatctgccgc agctttactg aaagaacagc
    16201 cagaaaagtt tgcctctgct ttgatattgc aattactgcc cggaacacaa ggattatatg
    16261 gttttgttat tggaattttg atttggttgc aattaactcc agaacttcct ttagaaaaag
    16321 gcgttgctta tttctttgta gctcttccaa ttgctattgt aggatacttt tcggctaagc
    16381 atcaaggaaa tgtagcagta gcgggaatgc aaatcttggc taaaagacca aaagaattca
    16441 tgaagggagc aattttggct gccatggtag aaacctatgc aattcttgcc tttgtcgtat
    16501 cattcatttt gacccttcgt gtataagaaa ttaatttgct attcaaagga ggtgtctaaa
    16561 tgagcaattt agaaaactta cgagagtctg ttattgaaca agctcatgaa aaagggcgta
    16621 tgaaattatt ggattccaaa aagaagattg atgatgaatt tgaaatgcaa aagtcggtca
    16681 ttgtaaagaa aaaagaagtg gaacatgaac gaaagttaaa agaattacaa cagaaatatc
    16741 aaataatttt tcaacaatta aaaaataagg aacgccaatc aacgttagta tcaaaacaga
    16801 aaatattaaa agaacttttt caatctgctt taatagaaat ggaatcttgg agtgcagata
    16861 aagaaatgga gttcatctat cgaattctgg aacgatattc acaacaagag gtcatagtaa
    16921 cttttgggga gcggacttta gctaaattca atctggaaca attagagaaa ttgaaattct
    16981 cttttccaaa tcatttattt agtgaacagc ctatctcaaa tgaatcaggc ttacttattt
    17041 caataggtaa aattgatgat aactatttgt ataaaacatt aattggatcg atttctaagg
    17101 aagaaagttc aagtatcgca aatcaaattt ttatcaatta aggatgaaat tggttaatcc
    17161 ttcttagaaa tttggagtat tccaataaaa ttagaaaggt attttatgga tactaatctt
    17221 ttttcaaaaa taaatacgac gatttcggta aaagaaaacg attttattac agaagataaa
    17281 tttcaaaaaa ttatacaatc caaagatacg gagacaatgg catttatctt agaagcaact
    17341 ccctatcatt tatcgattga cgttttagaa aatcctagtc agacagagac ttcgctaatg
    17401 acaaaattag tcaatgatta tagatgggcc tatgctgaaa gtccgtctaa taaaattgtg
    17461 actttatttg ctttacgata tgtttatcat aatatcaaag ttttattaaa atctaaggcg
    17521 gcaattaaga aagatttttc taaattatta attccaatag ggatttttga tatagaaagt
    17581 ttaaaacatt tagtttcttc cttacattca gatacacttc ctgattttat ggttcatgaa
    17641 gtagaatcaa tttggaatga gtatgaaact tttaataata ttcgtgtact tgatgtcgga
    17701 gctgatctag catattttaa acatctgaaa cttttatcta atgagttaga tgaggtgctg
    17761 tctcaggtta ttgtcgcaat gattgacttt tataatatta ttactgtaaa acgtggttta
    17821 tctcaaaata agagtcatgg ggatatttta caattacttt cagatgaagg aagtatttct
    17881 gctaaagaat ttatatacat tgtagaaaat caagaaatat ttgtgtggtt caataaaata
    17941 aatccaagct tagattcaat cttttcaact tatgaattga agatgcagga cgcaacaatt
    18001 tcatcttctg agttagaatt tttatgtgat ttattattgt ataaaatttt agatcaagga
    18061 aggtacaatg tagatgggcc gttagtactt gctagatatt tattgggatg tgagtttgaa
    18121 gtaaagaatc tcagaatgat tatatcagct cttcaaaata caattccctt tggatcaata
    18181 aaagaaagga tacgcccgca ttatggaagc taataagtat aaaattggca taattggtag
    18241 ccgtgatatt attttaccct ttagcatgat tgggtttgat atatttcctg cctatcaaga
    18301 acaagaagct ataaatactc taagaaaatt agcccaatct gattatggtg tcatttatat
    18361 cactgaagac attgcttcaa tgatattaga tacaattcgc cattatgatt ctcaagttgt
    18421 gcctgctatt attttattac cgactcataa acaaggttta aatttaggat taaaacgtat
    18481 agaggataat gtggagaaag cagtaggaca caatatttta taataatgta caaaattgtc
    18541 tgtaatatta ttctataatt tttggactta gtaaggagaa taactttgac tcaagggaag
    18601 attataaaag tatcgggacc tctagttatt gcatcaggta tgcaggaggc taatattcaa
    18661 gatatttgcc gtgtaggtaa gctagggtta atcggtgaaa ttattgaaat gagaagagat
    18721 caggcatcta tccaagtcta tgaggaaaca tctggtcttg gtccgggaga acctgttgtt
    18781 acaactggag aacctctctc ggttgaatta ggaccaggat tgatttctca aatgtttgat
    18841 ggcatacaac gcccattaga tcgatttaaa ttggctactc ataatgattt tctagttcgt
    18901 ggggtagaag ttccaagttt ggatagagat attaagtggc attttgattc cactatagca
    18961 attggtcaaa aagtgagtac gggtgatatt ctaggaactg tcaaggaaac cgaggtagtt
    19021 aatcataaaa ttatggttcc ttatggagta tctggcgaag tcgtttctat cgcatctggc
    19081 gattttacaa ttgatgaagt tgtatatgaa ataaaaaaat tggacggtag tttctataaa
    19141 ggaacgctta tgcaaaaatg gcctgttcgc aaggcgcgtc ctgtttctaa acgtttaatc
    19201 ccagaagaac cattaatcac aggtcaacga gttattgata cattctttcc agtaaccaaa
    19261 gggggagcag cagctgttcc tggaccgttt ggagcaggaa agacagttgt acaacaccaa
    19321 gtagctaaat tttccaatgt tgatattgtt atttatgtcg gttgtggaga acgtggaaat
    19381 gaaatgacgg atgtactgaa tgagtttcct gagttgattg accctaatac cggacaatca
    19441 attatgcaac ggacagttct gattgctaat acttcaaata tgcctgttgc tgctcgtgag
    19501 gcttcaattt atacaggaat taccatggct gagtattttc gtgatatggg ctactctgtc
    19561 gccattatgg ctgattcgac ttcacgttgg gcagaagcac tacgtgaaat gtcaggacgt
    19621 ctagaagaaa tgcctggtga tgagggttat cctgcttatc tgggaagtcg tatcgctgaa
    19681 tattatgaaa gagcgggacg ttctcaggtt ctagggattc cagaacgtga aggaactatt
    19741 actgctattg gagctgtatc gccacccggt ggagatattt cagaaccagt tactcaaaac
    19801 actttacgga ttgtgaaagt tttttggggg cttgatgctc cgttggcaca gagacgtcat
    19861 tttcctgcaa ttaactggct tacatcttat tcactctata aagacagtgt gggaatttat
    19921 atagatggta aagagaagac agattggaat agtaaaataa ctcgtgcgat gaactactta
    19981 caacgggaat ctagtttaga ggaaattgtt cgtcttgttg gaattgattc tctgtctgat
    20041 aatgaacgac taacgatgga aattgctaaa caaattcgag aagattattt gcaacagaat
    20101 gcttttgatt cagtagatac attcacttcg tttgcaaaac aagaagcaat gctaagtaat
    20161 atcctaactt ttgctgatca ggcaaatcat gctttagagt taggttctta ctttacagag
    20221 attatggaag gtaccgtggc agttcgagac cgtatggcgc gaagtaaata tgtttcagaa
    20281 gatagattag atgaaatcaa aattatttca aatgagatta cacatcaaat tcatttgata
    20341 ttagaaacag gaggtctata aatgagtgtt ataaaagaat acagaactgc tagtgaagtt
    20401 gttgggcctc ttatgattgt tgaacaagta aataatgtgt cttacaatga gttagttgaa
    20461 attcaacttc ataatggaga aattcgtcgt ggacaagttt tagagattca cgaagataaa
    20521 gcaatggttc aactttttga aggatctagt ggaataaatt tagaaaagtc taaaattcgt
    20581 tttgctggtc atgcactaga attggctgta tctgaggata tggttggtcg tatttttaat
    20641 gggatgggaa aaccaattga tggtggacca gatttaattc cagagaaata tttagatatt
    20701 gatggtcaag ctattaatcc tgtatctaga gattatccag atgaatttat tcagacaggg
    20761 atctcctcta ttgatcattt gaatactctt gtacgtggtc aaaaattacc agtattttca
    20821 ggttcgggct tacctcataa tgaattagca gctcagatag caagacaagc gactgtttta
    20881 aattctgatg aaaattttgc ggttgtattt gcagcaatgg gtattacttt tgaagaagct
    20941 gagtttttta tggaagaact cagaaaaaca ggagcgatcg atcgttcagt cttatttatg
    21001 aacttggcaa atgatcctgc aattgagcgt attgcaactc cccgcattgc tttaaccgcg
    21061 gcagagtatc tagcttttga aaaagatatg cacgttctag ttatcatgac ggatatgact
    21121 aactattgtg aagcgttacg tgaagtctcg gcagcgcgcc gtgaagttcc aggtagacga
    21181 ggctatccgg gatatttata tacaaattta tcaactctat atgaaagggc tggtcgctta
    21241 gttggtaaaa aaggttcagt gacacagatt cctattttaa caatgccaga agatgacata
    21301 acacatccaa ttcctgattt aactggatac attactgaag ggcaaattat tttgtctcat
    21361 gagttgtata atcaaggtta tcgtccacca atcaatgttt tatcttctct ctctcgatta
    21421 aaagataagg gatctggaga aggtaaaact cgtggagatc atgctccaac tatgaatcaa
    21481 ctgtttgcag cctatgccca agggaaaaag gttgaagagt tagcagtagt attaggagaa
    21541 tcggctttat ctgatgtaga taaattgtat gtgagattta caaagcgttt tgaagaagag
    21601 tacatcaacc aaggatttta taaaaatcga aatatagaag atacgttgaa tcttgggtgg
    21661 gaattactat caattcttcc tagaacagag ttaaaacgta tcaaagatga tttgcttgat
    21721 aaatatttac ctttggtaga agtttaatcc agaaatggag tgattatcta tggtacgttt
    21781 gaatgtaaaa ccaactcgta tggaattgaa taacttaaag gaacgtttga aaacagctga
    21841 acgtggacat aagttattaa aggataaaag agatgaattg atgaggcgat ttatttcttt
    21901 gattcgtgag aataatcgac ttcggaaaga agtagaaagt tatctaattg ataatctaaa
    21961 atcttttgca gttgctaaat cattaaagaa ctctctaatg gtggaggaat tattttcaat
    22021 tccatcgaaa gaaattgaat tatttattga gaaagaaaat atcatgagtg taacagttcc
    22081 tagaatgcat atgaatatta cttctcaaaa tgagaacagt gaatacagct atttatcttc
    22141 taatagtgaa atggatgatg tatttgctac aatgaatagt ttaattgata aattactaag
    22201 actggcagaa gttgaaaaaa cgtgtcagtt aatggctgat gaaatagaaa aaacacgtag
    22261 acgtgtaaat ggtttagaat attcgattat tccaaacttg tcggaaacta ttcattatat
    22321 agaattgaaa ctagaggagg cagaaagagc caatttagtt cgtattatga aagtgaagta
    22381 gttcctttat ttagattttt aattagatga acaaatatca gcttggataa ggcggtaagc
    22441 ctttctaagc ttttttaggc tctttgtcaa ctgtagtggg ttgaa
//
LOCUS       DAVBCR010000027        22195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCR010000027 DAVBCR010000000
VERSION     DAVBCR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.027"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ508_001009"
     CDS             complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ508_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2490022.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ508_001010"
     CDS             complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ508_001010"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2490023.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ508_001011"
     CDS             complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ508_001011"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2490024.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3864..5132)
                     /locus_tag="VQ508_001012"
     CDS             complement(3864..5132)
                     /locus_tag="VQ508_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2490025.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5252..5620)
                     /locus_tag="VQ508_001013"
     CDS             complement(5252..5620)
                     /locus_tag="VQ508_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2490026.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5613..5879)
                     /locus_tag="VQ508_001014"
     CDS             complement(5613..5879)
                     /locus_tag="VQ508_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2490027.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ508_001015"
     CDS             complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ508_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2490028.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEKNSI"
     gene            complement(9447..10016)
                     /locus_tag="VQ508_001016"
     CDS             complement(9447..10016)
                     /locus_tag="VQ508_001016"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2490029.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ508_001017"
     CDS             complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ508_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2490030.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11286..11480)
                     /locus_tag="VQ508_001018"
     CDS             complement(11286..11480)
                     /locus_tag="VQ508_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2490031.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ508_001019"
     CDS             complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ508_001019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2490032.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ508_001020"
     CDS             complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ508_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2490033.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVSMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14414..15172)
                     /locus_tag="VQ508_001021"
     CDS             complement(14414..15172)
                     /locus_tag="VQ508_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2490034.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15230..16411)
                     /locus_tag="VQ508_001022"
     CDS             complement(15230..16411)
                     /locus_tag="VQ508_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET2490035.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ508_001023"
     CDS             16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ508_001023"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2490036.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17117..17190
                     /locus_tag="VQ508_001024"
     tRNA            17117..17190
                     /locus_tag="VQ508_001024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17151..17153,aa:Arg,seq:tct)
     gene            17355..17480
                     /gene="comC"
                     /locus_tag="VQ508_001025"
     CDS             17355..17480
                     /gene="comC"
                     /locus_tag="VQ508_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2490037.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17501..18815
                     /locus_tag="VQ508_001026"
                     /pseudo
     CDS             17501..18815
                     /locus_tag="VQ508_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            18812..19564
                     /locus_tag="VQ508_001027"
     CDS             18812..19564
                     /locus_tag="VQ508_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490038.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19616..19687
                     /locus_tag="VQ508_001028"
     tRNA            19616..19687
                     /locus_tag="VQ508_001028"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19649..19651,aa:Glu,seq:ttc)
     gene            19693..19766
                     /locus_tag="VQ508_001029"
     tRNA            19693..19766
                     /locus_tag="VQ508_001029"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19727..19729,aa:Asn,seq:gtt)
     gene            complement(19807..20349)
                     /locus_tag="VQ508_001030"
     CDS             complement(19807..20349)
                     /locus_tag="VQ508_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2490039.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20528..21295
                     /locus_tag="VQ508_001031"
     CDS             20528..21295
                     /locus_tag="VQ508_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2490040.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21380..22060
                     /locus_tag="VQ508_001032"
     CDS             21380..22060
                     /locus_tag="VQ508_001032"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2490041.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6868 a         4086 c         3621 g         7620 t
ORIGIN      
        1 cattttctcc attctgttcc ctttagctcc taaactgcac aaaaagagag gctccagcct
       61 ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg atagtgcatg
      121 agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt taatttgtgt
      181 actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag tttcacgatt
      241 tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac gaacctcttc
      301 atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg caccaagcat
      361 agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt actctgtaac
      421 cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg tagtttggac
      481 attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt gctctttcat
      541 atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt atccgcctgc
      601 acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca agactagacc
      661 aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt gtgaaactgt
      721 cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa tatctgaagc
      781 atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca agacattctc
      841 taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat caacatcttc
      901 tgctaaaggc ttgttcttag cgtgaacttt caagattgct tcacgacctt taacatcagg
      961 acggccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag gatcaagtac
     1021 atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa aaccatccat
     1081 ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc cgagaccgac
     1141 tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg gtgctgcttt
     1201 tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa acatttctac
     1261 aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga ctgccttagc
     1321 aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg gaatacgggc
     1381 tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa ctagttcttg
     1441 tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt ctttatttgc
     1501 tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc ctcccatatt
     1561 tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg atacgagtag
     1621 attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt tatggtcagt
     1681 agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc tggtaaattt
     1741 ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt tacttgtttt
     1801 aggattttta tagacaccag aaacttcgat aacactacca tttggttggt aagttaattc
     1861 ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt gctgacttcc
     1921 tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa agataaataa
     1981 taaccataga aaaggatttt taattaaacc attattttgt tttttcatta aagattattc
     2041 tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat tacgataatt
     2101 ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa agcaggtata
     2161 gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg caattttaac
     2221 agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt gacctgtatc
     2281 aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca catcttgttt
     2341 aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca tcatgaagtc
     2401 catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa tagatccttt
     2461 taaaatccca actaagattg gattttttcc tgcataatct ttagttaatt gagcacctag
     2521 ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa tatcgttttc
     2581 taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta tatcattttt
     2641 cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt caccaaattg
     2701 ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa taaataaacg
     2761 tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc gatgacgaat
     2821 gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt ctaatggaag
     2881 tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta acacaagttc
     2941 atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt ggtactcttt
     3001 tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt ttaaaatctg
     3061 ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat tcagataagt
     3121 ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta aatcttccac
     3181 attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt cattgccaat
     3241 gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta agtaagaatt
     3301 tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa agtgaaaaat
     3361 tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta tctctccgac
     3421 tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa tcaagcgcat
     3481 aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg tcgcacctgt
     3541 ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg cttctgaaaa
     3601 ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca acttccttaa
     3661 ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag ctagaatcaa
     3721 ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca tggaatctaa
     3781 tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct tcttgagaaa
     3841 atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt ggctatctta
     3901 gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg agaatctgca
     3961 tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg agctacttta
     4021 acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa agactctagc
     4081 acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt agaagaaatc
     4141 aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga tttgaatgtg
     4201 gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt atcagattct
     4261 tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc ttttttagga
     4321 agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc agatacgtat
     4381 tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt ataatagaga
     4441 taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat accagcttct
     4501 tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata tttttcagat
     4561 aacatttctt gaactttact catccgatta tcttcttcag aagttgattc tttagctacc
     4621 catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt cttgtcgatt
     4681 gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata aggactattg
     4741 tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac ctctgattga
     4801 tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt aaatactgga
     4861 attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg agtcaatttc
     4921 ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata aaccatggga
     4981 gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta ataaatttct
     5041 ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt caaaaaactt
     5101 ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt cttgtaaacg
     5161 caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac tcaaaaattg
     5221 ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca ggaatcgtat
     5281 aaactttttc cctcgactta gaataatagt acttcgctcg tgtatatttg gcagcataat
     5341 cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca ctcaaagttt
     5401 gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc tgataactct
     5461 gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat acccctccca
     5521 taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg tattcatttt
     5581 gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac ccgtgtttca
     5641 ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt catctctagt
     5701 acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt aactttcaag
     5761 tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc tgctacttcc
     5821 tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc taatctcatc
     5881 cttattacct caatttatta ttgtaccatt tttatcttct ttatagaaga aaaatatcaa
     5941 atggaatttt tttccttaga ctcttttatc tctaataaac tttctccaaa aatcagcaga
     6001 ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc cattaatccc
     6061 ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa ataatcttga
     6121 gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt atcttttctt
     6181 tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat ctctaaaaga
     6241 taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg taactcttca
     6301 taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt atgtcgttga
     6361 tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc agcattccct
     6421 ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc ctctaataac
     6481 tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt tcctaaaaga
     6541 tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga gcccaattct
     6601 gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga tttttctgga
     6661 cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc tcttaactga
     6721 tataaggttg acaagcccat atggtccgca ttttcaataa ataaagtatt agcatttgga
     6781 atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg tccctcaata
     6841 aagtctaata gagtattttc caactggact tcactcattc gaccatgaac atatccaatc
     6901 gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt gtcaactttg
     6961 ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc atcacgaatg
     7021 acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt cggcggagtt
     7081 tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg agggattggc
     7141 gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt ttccttatgc
     7201 ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc aaacacaaca
     7261 tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt tttcaatttt
     7321 tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc aatattaact
     7381 gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa aaccgtcgtc
     7441 ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc acgcatagca
     7501 acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg ctgagaagcc
     7561 tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc aacataaggg
     7621 aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa acccttcaac
     7681 tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac ctggttctta
     7741 accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt tggagcttta
     7801 ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat agaaatttga
     7861 tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat ttcaatggtt
     7921 tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc ccctttttca
     7981 agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg aaaacgacgc
     8041 tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc atctacaaaa
     8101 tgaaaaccat gtctgagatt accctctatt aaatttacag attctttaca gatacttgtc
     8161 ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa tgttttactt
     8221 cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata tcgttcaatt
     8281 tcttctttta gaaaagaaaa ctgattgaaa aattcctgta taggatattg attgaattga
     8341 taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa aaaggtcact
     8401 ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc agaaaatgct
     8461 ttactattct gtaattcttc tgtaaagtac tgcgctaatt ctctttcaaa gacttcatac
     8521 tgattcatca atttttgata atcatcaaag aatattggag tatctttttc aatataatca
     8581 aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagaatc tgcatgactt
     8641 tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat aggtgataaa
     8701 gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt ttctctcaaa
     8761 agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga taattgtgtt
     8821 tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat tcgacaaggt
     8881 tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa ttcgccctga
     8941 gtttgtactt gagtaacttt ttgatagcca ttttccttta actgatggat aaacgcgtgt
     9001 tgagcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc attgggagac
     9061 ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt agatgaatca
     9121 gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga catcaaaaac
     9181 tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc caagatagaa
     9241 ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa cacaatccta
     9301 tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga taaaccaagt
     9361 attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg atcattttct
     9421 gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt tttctcaaaa
     9481 tttttctctt gtaaatagta ttttacagaa tcgtcaactt tgtcaacaga ctgtaaaata
     9541 ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga cataccattt
     9601 ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat atgttgaata
     9661 atagacttga taccattatg accacctgct gagccttttg ctcttaaacg aattttccca
     9721 acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa accatagtaa
     9781 gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg tttaaccaga
     9841 taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat cttatcgtgt
     9901 gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc aacattgtgt
     9961 tttgtttcaa aatatttatc ccctggattt cccaaaccta caagtaattt ggtcatttat
    10021 ttttcctttc aaaagccaaa agggttggaa tttttttcca acctaattga caccatttat
    10081 taatttttag acattaaagc ggaattctat gatgtcgcca tcttgaacga tatattcttt
    10141 tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt atttcactag
    10201 atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg agtggataat
    10261 accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa cttctttttc
    10321 accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg tcaacttatc
    10381 tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt catcatccaa
    10441 ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat tttctgtcgc
    10501 tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa ccacatcctc
    10561 gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac ctttgacaac
    10621 cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt ctaggactgg
    10681 tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt gcgtacgtgc
    10741 catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa gaattaattc
    10801 cagattaatt gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac gtccttgctc
    10861 gcgcatcaca ttttcatcat caaaagcacg aactacgtga acaatcgcat ctacttcacg
    10921 aatattggcc aagaatttat tccctagccc ttctcctttt gaagctcctt ttacaatccc
    10981 tgcaatatcc gtaaattcaa atgttgtggg aactgtcttt ttaggagtta tcatttcagt
    11041 tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat caatcgtcgc
    11101 aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata gtgttgattt
    11161 accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca ttctccgttt
    11221 tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc tctaactaag
    11281 ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc catcatgaca
    11341 acatgctcac aattactgca ttttattttg atatctgctc ctacacgtgt aatttcccaa
    11401 cgattagcct ttttaccagt tgacttgatt gtacaagcgt gtgatttttt catctcaaca
    11461 aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc ataaaagagg
    11521 cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga gctgcatgaa
    11581 gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga taaagctaat
    11641 agtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg ggttgaaact
    11701 aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta ctttaccaac
    11761 ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt tcacagtacc
    11821 attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta agtttaccac
    11881 attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat caggatagtt
    11941 tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga tttggttatt
    12001 ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa attcgcgtag
    12061 agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag tcaatttttt
    12121 ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt gactcaatac
    12181 gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa aggctgtttc
    12241 attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg ctgaaatttc
    12301 ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg atttgccact
    12361 ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagttacat caggtaaact
    12421 agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag aagtaattaa
    12481 caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt gaccatttga
    12541 accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata aaataggaat
    12601 ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa ataaattttt
    12661 attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta gaaagataat
    12721 agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc atatcaagtt
    12781 ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt atccacaaat
    12841 tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc ttgatcaatc
    12901 aaagatttta ttttggcatg agcatgaatg actgtggtat gatcttttcc cccaaattcc
    12961 ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat ggatacttga
    13021 cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat actaacacca
    13081 taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat ttggctaaca
    13141 tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc cttgattttt
    13201 tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg aacatttgaa
    13261 tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc taaatgttcc
    13321 gttttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggtgtgat agtttgtgtc
    13381 aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg ttttggacta
    13441 cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa ggtattgaaa
    13501 aattcttcct gagttgcgac tttttttccg ctgagtgact ggatatcatc gattaacaaa
    13561 agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct taggtgatca
    13621 agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt aggaatattt
    13681 tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc tggtcctcca
    13741 tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa agcggctgat
    13801 acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt ttcttttaat
    13861 cccgtatctg aataaggaat agatactaac tttggactat agtcataaag agttaaattt
    13921 gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat atagtgggga
    13981 gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt tagttgtttt
    14041 tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt ttcctctacc
    14101 ttgataagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag tctttcttgt
    14161 gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc ctcctttact
    14221 atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt ccacaagctg
    14281 tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa aagaagaaat
    14341 tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga gataaaaaaa
    14401 taaaagaaca gccttatttc aggctgttga taattctact atattcttct tgatttgaaa
    14461 aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca tctaatccga
    14521 gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt tgctgtttct
    14581 tttgtttttt ctctgtcaga agagcttcta atttccttac agaaatatct tcttctataa
    14641 tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa cgcgcatggg
    14701 cttgtgatag tttgccattt tctacttctg aaagaatctg ttctggcaag gaaagtaaac
    14761 gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt tcagcatggg
    14821 tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg tttaaatttt
    14881 ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa atctgtttaa
    14941 caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt ctctctcctg
    15001 caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga atgaccccat
    15061 tttctttgat agactgtgct agttcatcta gtttttctct atcaaattct tttcggggtt
    15121 gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc atgattctac
    15181 actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat taagattcta
    15241 aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg ttacgatagt
    15301 aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt aagtctgttg
    15361 atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca aggtgaccat
    15421 tggcaggcat attactttgt accgaacgaa caattacacc agatgtaaca ttacttggaa
    15481 tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc tggattccca
    15541 aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg atagcatcat
    15601 ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta gcaattttac
    15661 ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca gagttacctg
    15721 ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca tcttccgatt
    15781 ttaaggatac atttctattg agactggata cgataccttg agtgacagta tttgcatatt
    15841 cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac ttactagaat
    15901 caccaaactc agctactgtt gtcacttttt ctgaagagat tttgacgaca gcaatatcag
    15961 agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac aatcgaatat
    16021 ctactttgct ggcgccattt ataacgtgat tgttggtgac gatgtaagct tctttatcat
    16081 tctttttata aataactcca gatccttcac tagagattcg ctgagaatct gtgtcagtat
    16141 catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca gaaacaacag
    16201 catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag gcagtttgtg
    16261 taatagtact attgttgtta gagttgttta cactactttt ttgagttagt tgagttattg
    16321 aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat aattgaaacc
    16381 attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg aattactgaa
    16441 agtataaact gactagctta attataactt aaacacaaaa gttttacaca aactgtggat
    16501 aactcttttg aaattgtgat tttcttaatt gaaatctatt ttttattttg tgaataagat
    16561 gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt aacagttggg
    16621 aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg aatttctaga
    16681 tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa ggccagtgaa
    16741 tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa aattgctgac
    16801 cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga agaatttagt
    16861 aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat tattggggga
    16921 agtttaggat tatcatcatc tgtaaaaaat agagccaatc tttctgtcag ttttggtcgc
    16981 ctaaccttac cccatcagtt aatgagacta gttcttgttg aacaaatcta tcgcgctttt
    17041 acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt gaatttttgg
    17101 tagaataatt gtattaggtc tcatagctca gctggataga gcattcgcct tctaagcgaa
    17161 cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga attttcccgg
    17221 cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag aattttatct
    17281 aaaacgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga aaaaacattt
    17341 taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct ttgaaggaaa
    17401 aagacttaca aaagattaaa ggtggggaga tgaggatttc aagaatcatc cttgattttc
    17461 ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac ttggatttgg
    17521 gacagttatt gttcattttt taattattag tcacagttac cgtttaattt gtaaaggtcg
    17581 aataaataga aaagaattat acgtttttgg tgcttataca ttactaactg aaatagtact
    17641 tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat ttttatttcc
    17701 tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata gaggactatt
    17761 cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt ccgtaatttt
    17821 ctcctctata acaggagata attttgtttt acaatatcat ttcccattct ttttcgttgt
    17881 gacggtgtta acctattttg ttacattaaa aatcatttac tatttccatt tggaactagc
    17941 ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg ctttactatt
    18001 gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt tgaatagttt
    18061 tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct ttgcaatgaa
    18121 ttctcataaa gttcaaatgg agaaagagat tgctttgaag cagaagaaat ttgaacagaa
    18181 acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc gtggttttcg
    18241 acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg gtaatttaca
    18301 gaatttacaa tgaagtttta gtcaaagcaa atcataaatt gcgttcagat aagtacactt
    18361 actttgattt gaacaacata gaagactcag ctttacgaag tttggttgct cagtcaattg
    18421 tctatgctcg aaataatggt gtagagttta cactggaagt aaaagatacg attaccaagc
    18481 ttccaattga actattggat ttggttcgta tcatgagcgt tttattgaat aatgctgtcg
    18541 aaggatcggc tgatagctat aaaaagcaga tggaagtagc agttattaag atggaaactg
    18601 aaacagttat tgtgattcag aattcatgta aaatgacgat gactccttca ggagatctat
    18661 ttgccttagg attctccact aagggaagaa atcgcggagt cggattaaat aatgtgaaag
    18721 aactactaga taagtacaac aatattattt tagaaacaga gatggaaggc agtacattta
    18781 gacaaatcat tagatttaag agggaatttg aatgaaagtt ttaattttag aagatgttat
    18841 tgaacatcaa gtgagactag agagaatatt ggatgaaatt tcgaaagaat cgaatattcc
    18901 aatatcatac aagacaacgg gaaaagtccg tgaatttgaa gaatacattg aaaatgatga
    18961 agtaaatcag ctttatttcc tagatatcga tattcatgga attgagaaaa agggatttga
    19021 agtggctcag ctcattcgtc attacaatcc ttacgctatt atcgtcttta tcactagtcg
    19081 atcagagttt gcgactctaa cctataaata ccaggtatca gccctagatt ttgttgataa
    19141 ggatatcaat gatgagatgt ttaagaagag aattgagcaa aatatcttct acacgaagag
    19201 tatgttactt gaaaatgaag atgttgtaga ttatttcgac tacaattaca agggaaatga
    19261 tttaaaaatt ccttaccatg atattttgta tattgaaaca acaggggtat ctcataaatt
    19321 gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt accatgacag atattcagga
    19381 aaaggacaaa catactcagc gattttattc tcctcacaag tcatttttgg taaatatagg
    19441 taatatcaga gaaattgatc gaaaaaactt agaaattgtt ttctatgaag accatcgttg
    19501 tcctatttca agattaaaaa ttagaaaatt aaaagatatt ttagagaaaa aatctcaaaa
    19561 gtgattgaca attagcaaga aattgatata atggttatat ctgctacaga tagaaggtcc
    19621 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
    19681 cggactattt tatccgccat agctcagttg gtagtagcgc atgactgtta atcatgatgt
    19741 cgtaggttcg agtcctactg gcggagtata gataaaaggg aacacagctg tgttcctctt
    19801 tttgtatcaa tttgtatcac caagcatttt cataaggaag tctgttattt cttgagaact
    19861 ttcttttttt ccatgtgcaa tccaagtttg gcagacacca aaaagtgcat gagttagata
    19921 gatgctacta tattctaatt cagtggtatt tagattcagt tgcataaatc gcttttgtaa
    19981 atctgtacta agcatgatat gaagtttatt tcgtaagaaa ttttggattt ctttagtccc
    20041 attttcagaa agaagggcag ccagaagtgg ttctgactct agatattcaa aaacttctaa
    20101 aatagcgtct cttttgtgat gagcatgttt ttgaaaaata tattcaaatg tatggaatag
    20161 cttgctttga tagtgctcaa tcatatcata cttatcctta tagtgagtat agaagctgga
    20221 acgactaatt ccggcttttt ctactaattt gacagtagaa attttatcaa atggctgttc
    20281 catcagtaat tgtaccatag cattttcaat agttcgcttt gtttttaagc gtttgttact
    20341 ttcttgcata tttcctcctt gtaaacaaat tagactatat gtctaaaaat agatttttta
    20401 tcttgtaatt tagatttttt aatgtataat ctattatatc aaaattttag acaatatgtt
    20461 taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt taaaaaaaac caacctttat
    20521 tattgtcatg atcgggattt ctcttattcc agatctgtac aatatcatat ttttgtcatc
    20581 aatgtgggat ccatatgggc aattgtctga cttacctgtg gcagttgtaa ataatgataa
    20641 agaggcttcc tataatggta atactatggc aataggaaaa gacatggtgt ccaatttaaa
    20701 agaaaataaa accttggatt ttcattttgt agatgaagag gaaggaaaga agggattgga
    20761 agatggcgat tactatatgg tagtgacttt accaagtgat ttatctgaaa aaacaactac
    20821 attatccaat attcaatcga cagcagctta tcaatcattg acaagtgagc aacaaactga
    20881 gataagtgat tctgtatctc aaaattcaac tgatagtatt caatcggctc agtcaattgt
    20941 agctttagta caagatttac agggaagttt agaaaactta caaaatcaat cttctaatct
    21001 ttcgacttta aaaaatcaat ctaatcaagt atcacctatt acttctactt ctttgatagg
    21061 attgtcaagt ggattaacag agatacaagg agatgttact agcaaattag ttcctgccag
    21121 tcagtcgatt gcatcaggtg taaacgcata tactacaggt gttgataaag tttctcaggg
    21181 cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt agtttggata aactagtttc
    21241 aggctcaaac accttgacac aaaaatcttc tagattgaca gcaggagttg gttaattaca
    21301 atcaggatct gggcaattag cagacaaatc cagtcagtta ctttcaggtg cttctccatt
    21361 agagaataga gctaataaat tggcagatgg atctgggaaa ctagcagaag gtggaacaaa
    21421 gttaacttct ggattggaag atttacagac aggacttgct tctttaggac aaggactagg
    21481 taatgctagt gatcaactca aatcagtatc aacagaatct aaaaatgcag agattttgtc
    21541 aaatccactc aatctttcaa aaacagacaa tgatcaagtt cctgtaaatg gaatcgcaat
    21601 agctccttat atgatatcag ttgctctttt tgttgcagca atatcaacaa atatgatatt
    21661 tgcgaaattg ccttcaggac gtcatccaga gagccgttgg gcttggttga aatctcgagc
    21721 tgaaataaat ggtattatag ctgttttggc aggaattttg gtatatggag gagttcagct
    21781 tattggttta actgctaatc atgagatgag aatatttatt ctcatcatcc taacaagttt
    21841 agtattcatg tctatggtga ccactttaac aacgtggaat agccgtatag gagctttttt
    21901 cttacttatt ttgcttttac tacagttagc atcaagtgca ggtacttatc cacttgcttt
    21961 gacaaatgat ttctttagat ctattaatcc ctggttacca atgagctatt cagtttcggg
    22021 attacgacaa acaatctcta tcaacaagtc attttcctag ctgtcatact agctctattt
    22081 actggtttag gtatgctagc ctatcaacat aagaaaatgg aagaagatta aaaaaatcga
    22141 ccgattaatg aactgcaccc caaaagttag acagaaaaaa tctaactttt gaggt
//
LOCUS       DAVBCR010000028        21250 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCR010000028 DAVBCR010000000
VERSION     DAVBCR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.028"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(303..2030)
                     /gene="ezrA"
                     /locus_tag="VQ508_001033"
     CDS             complement(303..2030)
                     /gene="ezrA"
                     /locus_tag="VQ508_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2490042.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2112..4058)
                     /gene="gyrB"
                     /locus_tag="VQ508_001034"
     CDS             complement(2112..4058)
                     /gene="gyrB"
                     /locus_tag="VQ508_001034"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2490043.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4073..4636)
                     /locus_tag="VQ508_001035"
     CDS             complement(4073..4636)
                     /locus_tag="VQ508_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2490044.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4721..5275)
                     /locus_tag="VQ508_001036"
     CDS             complement(4721..5275)
                     /locus_tag="VQ508_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2490045.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5286..6509)
                     /locus_tag="VQ508_001037"
     CDS             complement(5286..6509)
                     /locus_tag="VQ508_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2490046.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6546..8996)
                     /locus_tag="VQ508_001038"
     CDS             complement(6546..8996)
                     /locus_tag="VQ508_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2490047.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9195..10124)
                     /locus_tag="VQ508_001039"
     CDS             complement(9195..10124)
                     /locus_tag="VQ508_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2490048.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10264..10551
                     /locus_tag="VQ508_001040"
     CDS             10264..10551
                     /locus_tag="VQ508_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2490049.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10585..11919)
                     /gene="ciaH"
                     /locus_tag="VQ508_001041"
     CDS             complement(10585..11919)
                     /gene="ciaH"
                     /locus_tag="VQ508_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2490050.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11909..12583)
                     /gene="ciaR"
                     /locus_tag="VQ508_001042"
     CDS             complement(11909..12583)
                     /gene="ciaR"
                     /locus_tag="VQ508_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2490051.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12692..15238)
                     /locus_tag="VQ508_001043"
     CDS             complement(12692..15238)
                     /locus_tag="VQ508_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2490052.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15362..15802)
                     /locus_tag="VQ508_001044"
     CDS             complement(15362..15802)
                     /locus_tag="VQ508_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2490053.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15777..16646)
                     /locus_tag="VQ508_001045"
     CDS             complement(15777..16646)
                     /locus_tag="VQ508_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2490054.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16688..17416)
                     /locus_tag="VQ508_001046"
     CDS             complement(16688..17416)
                     /locus_tag="VQ508_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490055.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17424..17903)
                     /locus_tag="VQ508_001047"
     CDS             complement(17424..17903)
                     /locus_tag="VQ508_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2490056.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17914..18612)
                     /locus_tag="VQ508_001048"
     CDS             complement(17914..18612)
                     /locus_tag="VQ508_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2490057.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18605..18871)
                     /locus_tag="VQ508_001049"
     CDS             complement(18605..18871)
                     /locus_tag="VQ508_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2490058.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18937..19863)
                     /locus_tag="VQ508_001050"
     CDS             complement(18937..19863)
                     /locus_tag="VQ508_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2490059.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19938..20753)
                     /locus_tag="VQ508_001051"
     CDS             complement(19938..20753)
                     /locus_tag="VQ508_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490060.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20757..21077)
                     /locus_tag="VQ508_001052"
     CDS             complement(20757..21077)
                     /locus_tag="VQ508_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2490061.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6421 a         4669 c         3720 g         6440 t
ORIGIN      
        1 cggttaccaa gttacttcaa agtacagatt tgaagctgca gataagactg acgaaatcac
       61 tttaatacta aatgaaaatc aaaaagcaaa ctaagaagct agcctcaggc tgtacttgag
      121 tacggtaagg cgacgctgac gtgatttgaa gagattttcg aagagtataa aacactgttt
      181 tgaagtggtg gataaatcgg ctgggtaaaa actcaatctc aggagaaaat gaagtaaatc
      241 tccccacaac tatagaaaaa gatttgattc tgctcctcac acaataaaat ctttttcttt
      301 tattaaaaac gaatcgtttc acgtgttttc tcatatgagg taacaaagcg attggttaca
      361 ccaggctctg ccacttccaa tgcttgagaa atcttgtcaa atgaagcgtg ataatcaaat
      421 tctttttcaa aaatatctaa agcttcgtta aatgcttctt gaatgcgttc atcaaatgag
      481 cgatagcggt tagaatattg caagagttgc tctgtcaaag ttgcatattg tacaatatta
      541 taagtttccg tttctaaagc ttccatatca ttcgttgcaa tttcaagaac tcgggtaaca
      601 gattcaatgt taatcatttt ttgttctaac tcaaccatta aatcctcggt attattgctt
      661 gccgtaaaga ataacttcaa gaaagtttgt ggaatacctg gcagattgcg tttttccatg
      721 tatcgcttga tagtatggag acgattgaca taaacattgg ccttttgacg tgcattaata
      781 tcatctttct caatttgtgt caggcgctca ctaactgaaa tttgctcatc ttcaatatct
      841 tttagttgag tttgtaaatc ctcaagattt tcttcaagaa ctgaataagc ttgggttggt
      901 tcttcttgat ttgaagttac ctcaacaata gctgcctcaa aactctctaa ttctgtctga
      961 atacgacgaa catggcttgc agctgtctca ggaagtaaat aggtcttgtt caaacgtgca
     1021 atatcttctc ccaataaagt attattctct ttcatatgtt gaaggtaagt tggaagagtt
     1081 gcaagtagat tttccactac tttctgagca gcaatttctc gagtaaaaat atcatacaag
     1141 gcattgattt cctcttgtgc ctgtccattc tcatattcgg cattatccaa ttccaactga
     1201 cgaatattct cttggttttt cttgaatgct tcataaagca agtggaaacg cgcttcaata
     1261 tccgtttcaa caaaatgata attagcatca attagtttac gataaccggc ttccaaatcc
     1321 tgtaattgat ctggcaattc tgtagaaagc gtcgtaacca aggctggaac acgatccaca
     1381 atatgactta aggccaaaat gtgattttct gtattatcca aaatcactgc ggcttccaca
     1441 gggtcacccg atgaattcaa ggttacaaat tgtgaaaatt cagattggat attttctaat
     1501 tgtttttcaa tttcatccaa ggcttgacca tactgttctg aattttcagc aactctatgc
     1561 tgaagttcct caaataaatc caaagcatga agaacacgac cactattttt agattcttgc
     1621 ttctctaagt ctgccaaagc attgcgaatt gccgcaatat cttcttcaat caaagtaatt
     1681 tgactctcaa tttggtcaat ttgatgactg gccttgagaa aacgaaatga atggttataa
     1741 ccttctgctt caaagagatt attttcaata tcggcaaaag agttgagaga taaatcgacc
     1801 catttttgat tccattcacg gaaagccact tgactttgtc caatcaagtg catatttttt
     1861 acagcttcta cttcatcatt tactggaaga ttgtatagtt cttcttttct ttcttctagc
     1921 gcctctaatc tcccctcgtt tcgcttacgt agaaagattg ccactacata agccagaact
     1981 aaaatgactg caattgcaac cattaaataa attagttgtc cattagacat atcaaactcc
     2041 ttttttactt gaaacaatcg taacgattat atcatatttt ttagaccaag ggaactactt
     2101 ctccctattt tttagacatc aagtgtacta tagacagcat tttcttcgat aaactcacga
     2161 cgaggctcta ctcgatcccc catcaacata tcaaagattt tatctgcttc tgcagcatca
     2221 tctacagaaa ctctagccat caagcgatgt tcgggatcca tggttgtttc ccacagctga
     2281 tgatcgtcca tttcacctag ccccttataa cgctgaatag tcggtttggt acgaccttca
     2341 ctataacggg ctaaagcttc ttggagtttg atttcttgat ctgcacccgg ctggatatat
     2401 tctttaatct cgcttccaac cttgacacca tagattggtg gttgggcaat ataaacataa
     2461 ccagcttcta ggattggttt catataacga taaatcaagg ttaaaagaag ggtacgaata
     2521 tgggctccat cgacatcggc atcggtcatc aaaacgagtt tttggtaacg ggctttcgaa
     2581 acatcaaatt ctgcgccaaa tcctgttccc atggctgtga aaagactacg aatttcttcg
     2641 ttagctagaa tcttatccat acttgctttt tcaacgttca aaatcttacc gcgaattgga
     2701 aggatagcct gaaactcacg gttacgacca gatttggctg atccaccagc tgagtctcct
     2761 tcgacgatga agagttctgt ttcagcaggg ttattagaag aacagtctgc tagtttccct
     2821 ggaaggttgg aaatttccaa accagatttt ttacgtgtga cttcacgcgc acgcttggca
     2881 gccacacgag ccttggcagc taaaatccct ttttccacga tacgtttggc aatctgtgga
     2941 ttttccatga ggaaatcgga gaaggcttca ctgaagaggc gattggtaat cttgaccact
     3001 tcgctatttc ccaatttggt cttggtttgt ccttcaaact gtggatttgg gtgtttaact
     3061 gagataactg cagttaatcc ttcgcgaaca tcttcccctg ttaaattatc ttcattgtct
     3121 ttcagtaact tattcttacg agcataatca ttgataacac gagtcagtgc cgtacggaaa
     3181 ccttgttcat gcgttccacc ttcatgggta tgaatattat tggcgaaact catgacattt
     3241 tcatggtaac ctgttgtgta ctgcatggct acctcaactg tgatatcatc catctcaccg
     3301 tctgtataga ttggtgtatc aaagattaca tccttgttct cgttgatata ttcaacgtaa
     3361 ctagcaatcc caccttcata atgataatgc ttggtttgtt ccaaaccttg gcgcttatca
     3421 gtgatagaaa tttgaagacc gcgatttaga aaggccaact cttgaatccg tttatttaat
     3481 ttatcaaaat caaaggttgt tgtttcagta aagatttttg ggtccggtat gaagtgaacc
     3541 gttgttccag ttctatctgt atccccaacc acctcaaggt ctgcaacaac atgaccacga
     3601 cggtattctt ggtaatgaat cttaccgttt ttatggacat gaacgtctaa ttgagtggaa
     3661 agggcattaa ctactgacga ccccaccccg tgaagaccac ctgaaacctt gtatccacca
     3721 ccgccgaact ttcctccagc atgaaggact gtaaagacgg tctcaacagc aggacggcct
     3781 gttttttcct gaatatcgac tgggatacca cgcccatcat ccacaacagt aatcgaatca
     3841 tctggctcaa taaaaacttg aatatggctg gcaaatcctg ccaaggcctc gtcaattgag
     3901 ttatcaacaa tttcccagac tagatggtga agaccttctt ttgaggttga tccaatgtac
     3961 atccctggac gcatacgaac agcctctaag ccctctaaaa cttgaatttg actggcatca
     4021 taatcctgtg cctgcagatt tttgatttct tctgtcatct tattcctttt tcttatatgt
     4081 ctaatacttg tcttaaattc acgatactgg taaacaagtg ggtatcaagt cctgcagctt
     4141 gacctgcttc gatatcaatc ggccgatcac caatgacaag accagagcta atctgatact
     4201 tttctcttaa ataaagcatg gattcgggat ttggctttct cttaaagcct gagctagaag
     4261 tcaccacttc tgtaaaataa gctgctatag aggttttttc taaaatttcc aaaacctgat
     4321 catttcgatg agagaccaaa aaatgacggc caccttgatt taaaatgtct tccaataggt
     4381 cagaaactcc ttcaaataaa atcgggtgtt caagctctct ggcttcattt tccttgtact
     4441 tttctaaaaa attctctaaa ttgggagcga atgtctcaat cgcaaaagga gtagaaacct
     4501 ttaaagcttg atagacactg tcatggtctt gtgtgatacc atacagtgcc aatgtttcaa
     4561 caaatgcagc tgttgaagtt tcataattat ccagtaaagt tccacctaaa tcccagatgt
     4621 aatcgtgata tttcataccc ttcattatag cattttttta tgaaaaaagc gatgatttcc
     4681 ctgagttttc cacataaaaa acgagagaat aactcccgtt ttactgattt ttaccaaaga
     4741 catctcgata gagcattcct gttcgtaaac tctctgcgtc ccctcctagt tgctccacca
     4801 actcgtaggc aaaggcaagg gctgttgaag gaccccgact ggttgtcaac tgaccatcta
     4861 ccactactgt ttccttgacg tagtgaccat caaggatttg ctcttgaacg ccgtcataac
     4921 aagtgtatcg cttatttttc aatatctctg cttgattgag ggcaattggt gccgcacaaa
     4981 tggctgctag tttcttccct tcttgctcga agctttgcaa ttcttgaatc aaggtctgat
     5041 tatcacgtaa atgtgcagaa ccaggcatac ctccaggaag aacaatcata tcatagtctg
     5101 ataaatctcc atcaaagaca tgatctgctc ttacttggat tgcatgcgaa cccgttactt
     5161 gctcttcaaa accaaccata tcacatgtga tattggctcg acgcaagaca tctacaactg
     5221 tcaaggcttc aatttcttca aagccctgag ctaacataac tgctactttt accatgattt
     5281 tctccttatt taatttgttt taatacaacc tttttccgtt tgaatgggac ttttccgctc
     5341 ttttcttcag ctagattagt ctggtaactc atcgataaaa gcaaaccaac accaatcaga
     5401 ttactgataa tagctgatcc cccttgcgaa atgaaaggca agggaatccc cgtcaaagga
     5461 agtagtccag tcacagcacc gatattctca aagatgtgga agagcaacat cataatcaaa
     5521 cctgtggaaa tataagtgta gaactggtta tttgatttaa gagtaatctt caacatacgg
     5581 taaatcaaca tgagatagag ggcaataacc aggacagagc caataaagcc aaaatcttct
     5641 gcaataaccg taaaaatcat atctgactct cgaactggga taagcagatt cgaagcatta
     5701 aatccctgac caaataagcc accactccca atggcaatct gcccttgagc ctgctggtaa
     5761 gtcgttgttt gggcaaactc aaagggattg agccaagcca aaatccgatt gatttggtag
     5821 gtcggcattc caatctggtg aagaaaagct cgtccgtcct tgctaataaa gatagctaag
     5881 aaaccagcaa ctcctgttac agcagtcaca aatactggga taataatttt ccaagaaacc
     5941 cctgataata aaacgattcc tgagaaaatg gctacaaaaa ccaaagccgt ccccaagtca
     6001 ctttgaagtg ctaaaagaac taggactgga atggtaaaga gaatcatcca gaaaattaac
     6061 aaaaagtcca gcgaaaccgt gcgtctccat tccttatgtt tctttgtaaa ttggacaatg
     6121 acacgagcca acatgaggat ataggatatc ttcataaatt ctgacggttg aaatagggta
     6181 attccattta ttgataccca gtttttggca cccgttgatg caactaagct tggattataa
     6241 aatacaatcg gcaagatcat aagtcccaag cctaaaatat atagaaaggg ggtcaccttc
     6301 caaagaaatt ctgtattaaa gagcatgacc acaaaaccaa tcacaagccc caaggcaatc
     6361 caggcgacct gctgccctaa aatgggcaga atattattgg gataatcatg actaacggct
     6421 atatagatag ccaccacacc gatgaccagt agaaaaaata ctggcaagag caaactataa
     6481 tcgactctag agtcgagaga acgtttcata tatactaacc ttatactttc atacaatact
     6541 atttatcaaa gttcattaaa aaatctatca atagcctcgt caacttcgga tcgagagatg
     6601 gttttaacag tcgcttcttc tgctagagat gctactattt gtttgccgta tcgttttccg
     6661 atgattctcc tatccaaaat aagagttaag gaacgttggt attcacgtct catacttctt
     6721 cccaaagcct gttttaaacg aataatggcc attggcaatt gataatcata aaaggcattt
     6781 ttcccttctt gattcagttc ttgattaatc tttttcgtca agggttcttg aggattttgg
     6841 aaaggaagcc tcggtacaac ttgaatcaca aaaggatggc ttgaaaaatc aactccctcc
     6901 cagaaacttg ctgcaccaag caagatttgt tgttcacctt tttcaaagcg tttctttagc
     6961 tgatgaacat ccccattttt atactgggcc aagtggctaa ctgtaagtaa atccgatact
     7021 gctagaagca tgtctttagc ggtaaagaga accaaaatcg gttgctggaa agcttgaatt
     7081 tccactagta aagcagctac ctctctggca tagacttcta aggaggtttc tgttaccaga
     7141 gggaaatctt tgaccataac cacttcttgt tcctgttttc cccgagattc aatcttgaca
     7201 aatttggctt caggatagcc taaaaggtct gccaaagaaa ccctctgact aatctcaaga
     7261 gtagccgata ctcccatggc ttggcaactc tcaggaagta aagaggacag aagagtacgg
     7321 cctgattttg tagaagaaat ctgaattttc ttttgactcg tttcagttac ttcaagccag
     7381 taatcacctt cagctgtaaa atagcgaacc agctcatcaa agccttctac ttctaattct
     7441 gaaaaatact gatggagatt gtccagagaa tctaagatat ttttcctaga tttaccagac
     7501 tgaaattgtt ctatcaagta gagacactca aagcgaatac tttctagtat ccgttgttga
     7561 accctgtttt cttctcctac taaagcctta tcaattaaat cgataataga ttgtatatcg
     7621 taggtctctt gaagcagatt ttctagagct aacaaaatct tttggacttc atcaataatc
     7681 agtaaacggt cactgacaaa ttcaggatta tcttcaagtc tggtcacgag ataggcatga
     7741 ttagtcacta aaagcttgca agtctctgcc ctttcttgac tacgtttcca aaaatcttcc
     7801 gtcacaaata agctctggga tgataaattc ccatcatgac gaaggtctgc tagaaaatgt
     7861 tggtaacgat agagttgccc gatttcatcc aaatctcctg tctctgtctc agtaagccag
     7921 accaagactt gcattttaaa gcgtctaaat aagcgatttt catcattttc ctgcaaggaa
     7981 tgataaaagg catccaactt cagataattt tgtggtccct ttaagctatg aatatctgta
     8041 tggaacactt ccttgaggcg tttaccttct tcttccatga tttgattttg aagaatcttt
     8101 gtcggaacac taagaacaat ttgtcgctct ttggattgag ataaagcggg taagagatag
     8161 ccataggttt tcccaatccc tgtcggcgct tgaatcagag agacaggttc atctttcaat
     8221 agcaagccaa cctctttagc aaaactttct tgttcctccc tcacttcaag gttcaacaga
     8281 gaaatatttt tagaaaagtc ttgagatagt tttcgtggct ccagagaagc tgccgttttc
     8341 ttaaaataca gaccttgaac ttggaccaag tctggagaac tcaggataga ttggttgcga
     8401 taagtttcct caataaccag gtaggactca tataggagag cgtcagccat ttccagcaag
     8461 cgttccaaga gacctttagg aagctgagtc atcttttccc gtaaaaaaag aagtaattct
     8521 gctgtagctt gggcatctga aagggctgtg tgtgcgtgtt taagaggaat tcctaattct
     8581 cgacacaaaa tcggcaagct atatttttcc agttcaggga aaaagacctg ggccaattcg
     8641 accgtatcaa cacgagggtt tcttagctca tagccttcaa aaaataaatt ttccgccaag
     8701 agattagcat caaactgaac attatgggct acaaaaatcc catcctccac caagtcaaat
     8761 atttttctgg caacttgcga aaaatcaggt gcttgcgcca gacgttggtc tgtcaatcct
     8821 gtcagttctt tgatatgagc atccaagggt tcatgtggat tgacatccgt cgtatagtga
     8881 tcgacgattt ctccgtcctc aatcacgaca attcccactt ggataatttt agccttacta
     8941 cctgtgctag ttgcctctaa atccaccaca acatagcgat taccaatcgt tttcattata
     9001 aaaaattata ccaaaaaaag ggccatttgg cacctgcaaa cataggataa ttttcaaact
     9061 caagaacact acttttctta aaaatcccat cgaatagagc attctagcct gctgattttt
     9121 taaaatagtg aagaaatacg aaaaattgag agaagaaatg ttttcatctt ccttctctca
     9181 acgaatcatg tattttattt aaccatgtca ggatcgtagt cataaaatcc tgtatcaagg
     9241 aaacggtttt tagggtgaag tttacgaact tcttcatcca gcaagaaggc ttggtcatgg
     9301 ctaaagttgc cctcttgaat caagctacgc gcttggataa agagttttgc aatctcaaca
     9361 aagttctgac caggagtgta gagcccttct agtttccagc gagtaaagcc atgctccacc
     9421 aattctgtta atttgatcat caaatcaagg tcattgttgg caaagatatg ggtcccatga
     9481 ttatcttcaa aaatggaata gtggctctct ggatcacttg gctcagccaa gaagaggtca
     9541 cgtttatgcg tcttttcatc atcgatatgt gtaaagttat agtagttttg caagagtgga
     9601 cgtttagaat gatggatgac gctagcaccg taaaccaaaa cttcagcagg aatttccaaa
     9661 atctctggca ttttgaaaag ttcagctgat ggaatttcac gcgccaaaac agcctcagat
     9721 gcgccagcct tttgtcccca gaagttaatc tgacgactgc tagttaccat ggttgaagca
     9781 tcgtagatgg tcttaaatga ataaccatcg cggttaacta cgtaaaagac gcctgcatcc
     9841 ccaatcgtaa tatagtctgt cttgatttct tccaagaagt ctaagaaagg cttgatacgg
     9901 tccatcatat cttggtgcat gagagcattg accgcaacga gcaattcctt accagcatca
     9961 tgaaccaact tagcgatttc acgtaattgg tcataactaa aggtcgttgg cagacgaagg
    10021 ccaaaatctt tctcaccgac atagatacgg tctacgccag cttcgagtag ttgttcaact
    10081 tgttcaatac tttcagcagt tgctgtaatg acaatctttt ccataagaaa aattatacca
    10141 catttctcca aaatcatcca tgctttgaaa atgaaaaggg actttattct ttttgtaacc
    10201 tttctgtaat aattctctgc cgaaaaaatg ataaaatagg tatgtactgt taaggagaga
    10261 attatgcccg taagaaaatt acaatcctat gaggtagact atcaagaaga attaaaccag
    10321 cagcttcctc attaccaagc ttatacacct gaagcgcaat ctgatgccaa tctcaaggaa
    10381 atcctatttt ttattaatat cgctgttttt tgtatctgta ttgctatctt tagttttatc
    10441 tttttagcat taaaattatc aactgctctt gcctttgccg cagcaatcgg attcagctta
    10501 cttgttttaa aagttcaacg gtctattatc aaacgaaaac gtagaagata aatccaaaat
    10561 atcgccccag ttggagcgat atttttattt tttcttttta gatggtgtct gaatggcaat
    10621 cttcacttca aagattgttc ccttgggttt attatctttg acagtaacag ttccttttag
    10681 agcatctaca atttgcttgg ctagggataa tcctaaacca aaaccacctt tttgccgagt
    10741 tctagccttg tctactcgat aaaaacggtc aaaaattttc tttttatctt ctgtcgaaat
    10801 accgattcca ttatcagaaa caagtaaata aagattgcga tcggtcgccg agataagaaa
    10861 atcaatttca ccatcctcct cagtatactt gacggcatta tcgaaaagaa tggtcatcag
    10921 ttgtttcaga agaagctgat ctgtgacaat tgttcgatgg atacgatttt caaaacggaa
    10981 gacacgatta ttttccgaag caatcatctc gtagtttgtg aaagttgtat taaaaaagct
    11041 agttggaact tctgcaagct ccggcttaat cccatcatct ctccgagcta agttcagcaa
    11101 gcttgtcgtt aaaaaacgca tatttcggac ttcttccaaa ctcgatgcaa tgctttcgct
    11161 cacatccata atggtagctt ctggcttacg aaaaagggtc tctaagcgat tttgcaaaac
    11221 tgcgagtgga gttcgtaact catgactggc attttccaca aaagactgtt gcttctgcat
    11281 actctcaagc aggggcctaa cactgaccct agctagatag agactggcaa gtaaagacaa
    11341 aatccagaaa ctagccatca cgaccacaat caattgctca tgcttttgac tagcctgttc
    11401 caactgactg gtattaatca agacagcagc atacttgata ttggttgaaa ccgaactaat
    11461 attggtctcc atcaaaatca cacgatagat ttcttcctgc ccatagctat taaaaacctg
    11521 aatctggtag atatgtccta gttctttctt ttctaactta atcttatcca agcccaaaaa
    11581 tcgatttcca gaaagaagtt gagtaaagtc tttatcaaag agaatgactt ccgtattgga
    11641 actgacatta ggttttattt ctactttact agcgtccgcc ctagcatttt ctaaatcttt
    11701 aatctcttct gttgccctat ttatagccag ctgaataact gcttgaggat tttcactcag
    11761 tccatgaagc ttatcgtcca ccgaagtata aagactcgaa tgcatgactt gtaaaataat
    11821 cagagtcatt gtagaaaaaa tcagggtgaa gacaccgaag ttgcggataa aataactaaa
    11881 gtcatccgca taccatgttt ttttaagttt actgaacatc ttttaaaaga tacccaacac
    11941 tacgcaaagt ttgcaaattc tctgcaaaag tggttccctt taatttctta cggacttttg
    12001 aaacatagac ttcgacaacc gaaatcgttg tatcactatc aaatccccat agacggtcaa
    12061 aaatctgcgt cttaggcaaa atcacatttt gattttgaag gaaataaact agtaaatcga
    12121 actctttccc cagcaattcg acaggagtat cttcaacttt aacggtattg gttgataaat
    12181 taaccacgat attcccataa gtcaaggtgt tttcattaaa cttccctgaa cgtttgagaa
    12241 gggcctgaat ccgcatttta agttcttcta ggtagaaagg tttggtcaga taatcatccg
    12301 ctcccagttc aaatccatgt cccttgtcat ccaaactttc cttggcagtc ataatcagaa
    12361 ctggtgtcgt aattcccttt tcacgcaatt cttttaagac ttggaaacca tttttttctg
    12421 gcaacatcaa atccagcaaa atcaagtcat agacaccact ctcagcttcg tagagacctt
    12481 cttctccatc aaatacctgc ataacatccg caaaatcgtc taaaaagtca aatactgaat
    12541 ttgacagacc taggtcatcc tcaaccaata agatttttat catgagaaac tcctccttat
    12601 taaaactatt ataccaaatt tgccttaaaa aaaactcaac tctctgcatt ttacatgaga
    12661 tagctgagtt ttctttttat tttaggctta tttatgcatt tccgtattga agaacaactg
    12721 cttcgactgc agctttttca cggctaatca agtcaacacg cgctgcaatt tccttgattc
    12781 ccataccgat gttacggcta agagcaaggt cagaaagttg cggctcaaag aactccttgt
    12841 attccgccaa gcgttgctga gtcttaaata catgagcagg aaggataaca aagctatcaa
    12901 agctcatatc tcctccaagg gctgccttaa tccaagccca gttttcacgc gcccaagacc
    12961 aagctgtttt ctgagttgct tgatgagcta ggaattggta ataccaagca gacaagtcct
    13021 gtggtttgac cacaaatttg tccttccaag aagtaatcaa gttttggata ttatccgcat
    13081 ctgtactgta tgcaagagct gctgccaact ggcgtttaaa gacagcatct gttgcgtgag
    13141 tataagtatc aagataaagt gctaacaagt ctttagtctc atgatgtttc atctcattaa
    13201 tcagaacttg tgaacgaata gctgctggga gtcctgcaag attctccttg tgtgttgcga
    13261 agatttggct agcgacttga ctagcttctg catcatttga gcgaatcatc atcgaaacag
    13321 ccagctgacg aaccaattca tcctcatctg attctccgtc tttagcttca aaaccaagac
    13381 ggtcatagtt atgacgagcc aatttagcaa ccagtccttt gaaggctgtt tcagcatccg
    13441 ttccttcatc aataaagcgc tcaagggctg aaatcacttg agaaacagct gaaaccacca
    13501 gataagactc ttccttagca agtttatcaa gaactggaag caagtctgca taagcaatgt
    13561 gccctgcctc agccaacaaa cgacgttctt gaacaatttg cagtttgctt gtgttatcaa
    13621 gtgtctctag ctcagcaaga acagcttcta acaagtctcc ttgatagtcg gtaatatagt
    13681 gggcagtatt ttcagtgttg agacgaagag ctccttcatt ttcagcaaga agagctgcgt
    13741 agccagggat ttcgatactt tcagtttcga gtgtatcagg caagcctttc cagttgctat
    13801 tgaggggcac cacccagaga cggttcttgt cttcgttctc accgatgaag aattgttttt
    13861 gtgaaatctt caagacatca ttttcaactt taacagtaag aactgggtaa ccaggctgtt
    13921 ccaaccaaga atccatgaag gctgcgacat cacgtcctga cgcttgacca agggcatccc
    13981 aaaggtcact accaatggtg ttgctgtatt ggtgtttttc aaagtaggcg tgcaaacctt
    14041 tagcaaaatc agcatctcct agccaacggc gaagcatgtg catgagacgg cttcctttgg
    14101 catagacgat agcgccgtca aagagtgtat tgatttcatc tggatgttta acttcgacgt
    14161 ggacagactg aacgccatca gtagcgtcac gttcaagagc aagaggtact ccacctgttt
    14221 ggaaatcttc aaagatattc cagcttggtt cgatggtatc cacacagacg tattccatca
    14281 tattagcgaa actttcattg agccaaaggt catcccacca tttcatagtc acgaggttcc
    14341 caaaccattg gtgagccaat tcatgggcca caacaagggc aacttgttga cggctagcaa
    14401 atgtagagtt ctcatcgaca accaagtaaa cttcacggta ggtcacaaga ccccagtttt
    14461 ccatagcacc agctgagaag tcaggaaggg cgatgtggag agattgagga attgggtact
    14521 taactccata gtaatcttcg taaaactcga tagagcgaac agcgatatcc agtgagaaat
    14581 caagatttga aagtggatgt gctttggttg agtagacacc taccagggta ccatttttag
    14641 ttttagcggt caccccttgc aaatcaccag caacaaaggc caacaagtaa gaagacatgc
    14701 gaggtgttgt ctcaaacttc cagatacctg tttccttacg gttttcaaca tcgatttctg
    14761 gcatgtttga caaggccaat tcaccttctg cttggtcaaa gcgaagagag aggtcaaaag
    14821 ttgctttggc ttcaggctca tccacacatg ggaaagcttc gcgcgcaaaa tggctctcga
    14881 actgagtaga caagacctcc ttcttgactc catcaactgt ataataagaa gggtaaatcc
    14941 ctgtcatgtt gtctgtaatt ttaccagaaa aggcaagaac caattcaact tgaccagcct
    15001 cagccaattc gatatgaagg gcttcattgt catggtcaac tgtaaatgga cgagcttgac
    15061 ctgcaacttc tacagaggtg atttccaagt ctttttggtg gagggagatg cggtcactct
    15121 gtgcttgacc agtgatggtc actttcccag aaaaagtctt ggtctcacga ctcaaatcta
    15181 aaaataaatc ataatgttca ggaacaaatt gcttaataaa atgttcaact gcttgcatag
    15241 ttttctccta ttctaagttt aagagctact gctcttcttc aaacaaactt attatatcat
    15301 attttgcttg agaaaaaagg attggacagc gatttccaaa actttcttcc ttctaaatag
    15361 tctacagtgg gtagaccttg cgaaattctt ctaaaacaac cttgctttca ggtgtaaaag
    15421 tcagccctac ttcacccaaa cagtctgtga aaaagtcctc atgaacctgg cgccaatagg
    15481 caagtgattt gtctccctca ccttccttaa aggcatgttg cgcagaaact tgattgaaga
    15541 gttcaacaga aacctttgta atttcgacaa tgcagacaga ctgattttga ctatctaaaa
    15601 tgacatcgaa ggtccctact tggggaagtg gttcgtcttc tagtacatag aggtcatagg
    15661 ctgatgctgt tgctgtcttt tctcctttaa acaccaaatc cgctaaaagg tctggttcaa
    15721 ctccaaaagc ccaagcatcg atttcatctc cgatcaaagg attgatttgc ttgtatttat
    15781 tccacatttc ttgcggtatc atgggtactc ctttgtaatt ttttactttc ttcttttatg
    15841 tgtttaagat gatctggatg gtcaatctct aaatcaaaaa tctctggaat agaactgtag
    15901 tggataatgc acttgatacc caactgattc attttttgta tgaaagaagt attcagatag
    15961 cctgctacag caaaattaat cttgttcttt cttgctttat cctgcatatc tcttagcata
    16021 tctaacatta ttggactttc cagatcatgc cattgactgt ttctcatagt cgcaaaaaca
    16081 aaggaagtca aatcattcat tccaactaca atctttgaaa tgcccgtttc cagtatacta
    16141 gataagtcaa aatacgctga cggtaattca atcatcgttc cgactttccc agtaaagcca
    16201 tactgacgca atactgtaat agcttgtttt aattggtcgg catcattgac aaaaggaaag
    16261 ataacagata gattggggtt ggtttgataa acttctgtaa cgacatgtgc ttcagcctga
    16321 aattcatcca aacacgccag taaacgcctg gttcctctat agccaaacaa gggatgccct
    16381 tcgtcaaaaa actctttagt ccccactaaa caattggctt ctgtattcgt taattcagta
    16441 aaacgatacc aaacttcttc atctgagtac agggagcaga tagtctctag ataatctttt
    16501 acaaattgct gacaacttgc taatatgatg ttttgattaa gctctctcaa caaatattcc
    16561 ccacgaatca tgccgacgtg gtgaaatagt tgaggataaa ttttttcaag aattttttcg
    16621 ccactaaggg caagttgatt tctcatagcc attcacctcg tcttaaaaga tacaatatca
    16681 tatataatta tatcaaaatt tcctctagtt caaaagcttc ataaggttca gataactcat
    16741 atcctaaaaa ttctgctact tttttggaag cctcattatg agcatcccaa agtggaaaca
    16801 tatctctatt taaactctct agaatcattg cagcaccaag ctttttagca aatccatttc
    16861 cttgttcgtt tggtctagtt gcaacttcca cttcaactgc tttacggtaa actaaccctg
    16921 aggaaatccc agcaatcagt tcattatttt taagaatcac aaagccaaat ccacctttta
    16981 aaacaaaatc ctgataggac tcaaaatccc cctgtaaatc ctgtgaccat tcttccgtag
    17041 aaaagcagtt ataaatatga ttatcaatag gcacaatatt gtaaccttcc tctaaatgag
    17101 taactagatt atttaaaaat tcaacttgaa aatttgcctt atctttaaaa gaatagcgag
    17161 taaaagaatt tagccccact tttcgatgca gaaaatcttg ccaattagta ttttctgaaa
    17221 taataatctt ataatctaaa ccatattttc taacaaaatc ttcccaaaat ctggaatcca
    17281 actcccctgc taggtataga aaattcccaa catcataaaa gcttgttgtc ctatcattgt
    17341 ttcggtagac agttccaatt tgagatttaa gaccatagag aaccatattt ttcttccatg
    17401 ttttaaatag ttccattatc cctctacttc accttccaat tcatgtaaga agtcttgtcc
    17461 agttctggaa atcctaataa ttcagactta accttcaaga ctaatggcga tgcattttct
    17521 tctgtaatct cttgaatatc catccaaata tatccaagtg aatcattcgc accatcagac
    17581 acagcttccg aagtcgtaac ttgaggtgca ctctcattca tttcaacatc atacaaggcc
    17641 atgacatggt caaccataaa atttgttaac tcttctctga cgaaaacatc gtagattcga
    17701 ggattagagt agcttctaac agtaaatccc gtctcttcca taacttctct agtcagcgtt
    17761 tccgtcagtc cttcaccaag ttgctgactg cctccaggta gatcataccg atgttgataa
    17821 gggcctctcg ttttttcaat gcagagtaac ttcccatttt ctaagcaaac agcgtagact
    17881 ccaaagtgtt ttttgatttc catccaactc ctcctacttc aaagaccagc caccatctat
    17941 tgtcaagatt tgtccttgca tggcgctcgc ttttccactt gctaaaaaaa gactaagctc
    18001 tgctatttcc tctggctcaa tccagcgctt gattggagtt tcactagcca cccagtcagc
    18061 caaaccacct ggttcaaaat ccgcagcggt catagctgtc ttgactgctc ctggagcgat
    18121 accaaagacc tgaatcccag cttcagcata gtctagagcc aactgcttgg tgaagccagc
    18181 caaggcatgc ttggatgaag tataggcgtg accacctcca cctgctaggc tagaagcaat
    18241 ggaacacata ttgatgatga ttcccttttt attttccagc atttgtgtca aataataccg
    18301 agtcaactct actggaataa tgtagttgat ttcaaaaatc tcttgaatgt cctgcgccgt
    18361 ttgttccaac agtggtttgt aatcatccaa aactccagca gtattacaca aaacatccac
    18421 ctgagggcac cagtcaaaaa taggttccaa gtccaaggtc aaatctctct gtaaaaagtg
    18481 aaaatcaccc tctaagagtg gcttttcacc ttggtcaact ccataaactt gatagccctt
    18541 ctctaaaaag aggcgagctt gagccaatcc gatccctgaa ctcactcctg taatgagtac
    18601 acgtttagtc atgcacttct acccaatccg ttgccaaaac atcacaaact gtcgggctcc
    18661 acatggaaaa accttctcct tcgccagaaa cgttgattag gaaataaggt gtcatttcaa
    18721 gtgcaagccc attttgctcg atggtatcaa agagttggac atagttttcc gcacctcccc
    18781 aaccagttcg tacatatttt ctcttagcct ttaacccagg caggatctct tcaaatgtca
    18841 tgtttttctc ctttaattct acattcttca tttaattata gcaaaaaacc gctttatacg
    18901 gctttttgaa tgtgagttat tcaaacctgc tactacttac ggcaaattat tccctgcagc
    18961 aagataaatt tcataccatt cttttcttgt taagctaaag tttgccgctc ggctaacttc
    19021 tctcaagtgc ttaggatttg ttgtacctac gactgcctgc atttttgctg gataacgcaa
    19081 tatccaagaa atggcaatag ttgaagaggt tactccatat ttaatagcta aacgatcaag
    19141 tacttgattt aaagcttgaa atttctcatt tccaacaaaa ttccctttaa aatacccgaa
    19201 ttgtaagaca gaccatgctt gaatgaccac atcgtgtaat tggcaatatt caaaaatgct
    19261 gccatctcgc atagctgctt gactatcttc catattaaca tgaaaagctg attcaaatcc
    19321 tggagtaaaa gccgcactca attgtagctg attaacagct aacggctgct tgacatcttt
    19381 tttaagcaac tccatcatca taggattttg attagaaact ccaaaatctc gaactttacc
    19441 ttgtttataa aggagattaa aggcttctgc tacttggtca gattccatca aagcatctgg
    19501 tcgatgaagg agcaagctat ctagatgatc aatcttcaat ctttgcaaaa taccgtctac
    19561 tgattttata atatagtcct tagaaaaatc aaaataggta aattcttcaa tgcgaatgcc
    19621 acatttggac tgaatccaca tcttttctct taaatctgga cgatttttta ggacaagacc
    19681 taacagttct tcacaacgac cacgaccata aatatcagcc aagtcgaagg cattgattcc
    19741 aacagaaagt gctgtttcta caagctcttc aacttctttt acagacttat cttttattct
    19801 catcattccg agaacaattt ctgataattc tttgtcatct tgaccaagag ttatgtatct
    19861 catcaaattt ttctccttta atttctaaca ttcttccctt cattataaca aaaaaccgct
    19921 ttgcaacggc tttttgacta tacttcactc cattttatct tcttaaaccc acggaacaag
    19981 acaaagattc caataaagag gacagctaaa ggaataactt ttgtaaggaa aacatttgaa
    20041 attcccatcc actcataata acggagcaga gaacccacca caagatgggc aataatcata
    20101 ctgacaaatg gacgaaagac cgcttctttc caattccaaa taccgataac tagcgaaatc
    20161 gtaaagacag acaaactatc ccagggagcc ggaatataaa aggctccttc ttgtatgaag
    20221 cttgccattc ctacatatcc taaaacaact agaagaacta tagtcccaac aacaatgtaa
    20281 gtgccaattt tcattttagg agaatcttgg actaaacttc ttcgtaaaat tgtggccaca
    20341 agtccaaatc caatcagaaa aataagaagt tgccctaaaa atgtgagcaa attgactgtt
    20401 aagagaggac ctttagaaaa atcacttagt agttgataat aacgtaatac agccaggaca
    20461 agaattggcg tcaaaaggga ctctttgata gaactgcgag gtgctccctt gagaatctct
    20521 ttcattattt ttttaggatt cttacctaga taatcctctg cactcatgcc atctcgttct
    20581 gcttctgaga aatctagcat catcaaatag atctgctctc tgagatagtc ttcatcatag
    20641 agaaatccag caagattaaa actttcccac aactcctcaa aatacttttg attctcctca
    20701 gaaaactcat gtagcaaagc gcttgtttct tcgtaatact tcattttctt catggtttaa
    20761 cccccattct taatcccttc tactttttga ctcaaatcgt cccattgctg ccaaaagact
    20821 gagacacgct cttctccttc tttcgttaat gaaaaatact tccgatctgg accatctggc
    20881 gacgggcgca tgtcgcctct tatccattga tttttttcta acttttgcaa caaaggataa
    20941 atagttcctg gaacgatagt atcaaatcca gcctctcgca aagtctgaac caactcataa
    21001 ccataccgct ctttttgacc aatcatatcc aagacacaac cttcaagaac accttttaat
    21061 agctgagttt ctttcatcac ttctcccttc taatctattt tgtaatacct actagtaact
    21121 tcacctatag tatattactt ctacactagt ttgtaaagca taatagttaa tactcttcga
    21181 aaatctcttc aaaccacgtc agcgtcgccc taccgtatgt atggttactg acttcgtcag
    21241 tttcatctac
//
LOCUS       DAVBCR010000029        21229 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCR010000029 DAVBCR010000000
VERSION     DAVBCR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.029"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(163..777)
                     /locus_tag="VQ508_001053"
     CDS             complement(163..777)
                     /locus_tag="VQ508_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2490062.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1592..1861)
                     /gene="rpsO"
                     /locus_tag="VQ508_001054"
     CDS             complement(1592..1861)
                     /gene="rpsO"
                     /locus_tag="VQ508_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2490063.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2087..2632)
                     /locus_tag="VQ508_001055"
     CDS             complement(2087..2632)
                     /locus_tag="VQ508_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2490064.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2871..3071
                     /locus_tag="VQ508_001056"
     CDS             2871..3071
                     /locus_tag="VQ508_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490065.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3502..3642
                     /locus_tag="VQ508_001057"
     CDS             3502..3642
                     /locus_tag="VQ508_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2490066.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3649..3825
                     /locus_tag="VQ508_001058"
                     /pseudo
     CDS             3649..3825
                     /locus_tag="VQ508_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3883..5826
                     /gene="thrS"
                     /locus_tag="VQ508_001059"
     CDS             3883..5826
                     /gene="thrS"
                     /locus_tag="VQ508_001059"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2490067.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5875..6849)
                     /locus_tag="VQ508_001060"
     CDS             complement(5875..6849)
                     /locus_tag="VQ508_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2490068.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6842..7519)
                     /locus_tag="VQ508_001061"
     CDS             complement(6842..7519)
                     /locus_tag="VQ508_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490069.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7675..8748
                     /locus_tag="VQ508_001062"
     CDS             7675..8748
                     /locus_tag="VQ508_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2490070.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8839..9141)
                     /locus_tag="VQ508_001063"
     CDS             complement(8839..9141)
                     /locus_tag="VQ508_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490071.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9209..9646)
                     /locus_tag="VQ508_001064"
     CDS             complement(9209..9646)
                     /locus_tag="VQ508_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2490072.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9722..10564)
                     /locus_tag="VQ508_001065"
     CDS             complement(9722..10564)
                     /locus_tag="VQ508_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2490073.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10676..11326
                     /locus_tag="VQ508_001066"
     CDS             10676..11326
                     /locus_tag="VQ508_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2490074.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11514..12148
                     /locus_tag="VQ508_001067"
                     /pseudo
     CDS             11514..12148
                     /locus_tag="VQ508_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12279..12716
                     /locus_tag="VQ508_001068"
     CDS             12279..12716
                     /locus_tag="VQ508_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490075.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12811..13110)
                     /locus_tag="VQ508_001069"
     CDS             complement(12811..13110)
                     /locus_tag="VQ508_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490076.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13518..13880)
                     /locus_tag="VQ508_001070"
     CDS             complement(13518..13880)
                     /locus_tag="VQ508_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490077.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            14239..14653
                     /locus_tag="VQ508_001071"
                     /pseudo
     CDS             14239..14653
                     /locus_tag="VQ508_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14692..15135)
                     /locus_tag="VQ508_001072"
     CDS             complement(14692..15135)
                     /locus_tag="VQ508_001072"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2490078.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(15165..17387)
                     /locus_tag="VQ508_001073"
     CDS             complement(15165..17387)
                     /locus_tag="VQ508_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2490079.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17798..18040
                     /locus_tag="VQ508_001074"
     CDS             17798..18040
                     /locus_tag="VQ508_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490080.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            18380..19009
                     /locus_tag="VQ508_001075"
     CDS             18380..19009
                     /locus_tag="VQ508_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2490081.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(19132..21024)
                     /gene="pepO"
                     /locus_tag="VQ508_001076"
     CDS             complement(19132..21024)
                     /gene="pepO"
                     /locus_tag="VQ508_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2490082.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         6277 a         4743 c         3822 g         6387 t
ORIGIN      
        1 ttatttcatt ttactatatt tataagaatc tcctaatcaa atcaaaagcc actctacaat
       61 agagtaactt taagattgtc catttatact caatgaaaaa gccctctgaa aatcagagag
      121 ctgatttgag ctcggactaa aatcctagtg aaacaaaaaa atctacacga tcagaaaagt
      181 ctctatcgtg ccattttcat acatgatgta tagtccaagt agaatgaata ctaagggcac
      241 aatgattcgc tcgtattttt caattgtctc gaatattaac ggaatagaag ataacacccg
      301 actaatctcg caaaagataa ttatgccgat tacaaacaca agcaaggcca cgagggtctg
      361 tgaccaatct aacgaagcaa aataaggtat atagatacct aaattatctc cgccagacgc
      421 aattgtcagc aatgtaactg tccaaaacag ttgatttgcc ttgctttgtt ctaatctttc
      481 aataatttct tcctcttctt cttccgcatc ttctccaaca attgcaaagc gaatccctaa
      541 atagataggg attaaaccaa gcaatccaac catccattct tcaggcacga aattaacgac
      601 ataagcagca actaaactcg cccctacaag taagcctgtg cctagatatt gccccgcata
      661 aatatgccat ttctgtttat tctgtgatag ctgtgcaaat aaaataatta aaataattaa
      721 ataatcgata ctggtggaaa tataaacacc aatagcagat atgattgtct gtcccataaa
      781 aaaaactcct gtattagtca gtaataaatc agcagatttt aggagagcac agacacatta
      841 atttagctat cgctagtggg taatatcgaa cgtaggaaag cattttactc ctcctcaaaa
      901 cgtagttaca aagtaatttc taactggaat tcagtgacta cttccatgta aagtataaca
      961 tactatactt tactttgtaa agagtgggct cttctagggg gcttgcatac aactactcgg
     1021 cttgaaaagc cgagtaactg cgcaccttta tggtgtaatt agctgatacc gtttgaggtt
     1081 tttgtagtct ccaaaattgt taccgataaa acctcaataa aaaagccctc tgaagtcaga
     1141 gagctgattt gagtccgggc taaaatccta gtgaaaaaga tgaaactcct tgtgctcatc
     1201 gaacactgtg tcatttccct attttcatac ggatttttga cgcccttagc atcatgattc
     1261 ttgctagata aatcgtttat agactaggcg acgaggcgaa gatcgtacga aactttcagt
     1321 gaaacaaaaa aaatctcccc gaagggagaa aagctggttt attttacctt acagtgctag
     1381 gaaccgtaac caaagattat acttgagtat atcgaggtaa ggtgacaaca caaaaaagct
     1441 acccaattta gagtagcttc attatcaaga tatgctcaat tgaacacggc ctaagcactt
     1501 ggcaaaaaag ataaaatctc ctagaaactg aagtttcttc gtaaattttc ctatttttac
     1561 tttgtgcttt tgacagcctt tgtatcttga attagcgacg aagtcctaga gagttgatca
     1621 actcacggta acggttaacg tcgtttttac gcaagtatgc aagcaagtta cgacggcgac
     1681 cgattttttt catcaatcca cggtaagtag cgtggtcttt tttgtgttgt ttgatgtgtt
     1741 cattaaggtg gttgatttcc caagtaagga cagcaacttg aacctctact gaacctgtat
     1801 caccttcgtg acgtgcatat tgtgcgatga tttcattttt tttctctttt gagattgcca
     1861 tgatgtttct cctttttatt tggcttcatc cgagtgacag gttggcatgc ctgtaaccaa
     1921 gaagaagtta tttgtcttta cgacaatccc tattctacca agatttaaat tttctgtcaa
     1981 acattaatac tctcatagtt attatttttc aagtaaagag atacgttctt ttatatgatg
     2041 acctttttca agttcatcta ccaatccaat agcataatct gcataactaa tctggcttat
     2101 tccttttttc attagtagta aaaatttctc ctgctagaat gtagtttcct gttttttcac
     2161 catcgggaat aaaatctaca gcaggactga caaaagccca gttgagatta ttttttgtac
     2221 gaagaagatc caattcatcc gcttgagctt ttgctagtga cttaaattct tcaggaaaat
     2281 caggcgtatc taaaagtcgt gtcgtttttg tttcatctat ataaaggctt cctgatcccc
     2341 ctacaactag aaaacgtgtc tgtgttccag caagaagctg attaaatagt tcgattgatt
     2401 tgctgtggag cggtagcgta tctggtgtat aagcaccaaa cgctgaaata acagcatcaa
     2461 atccagtaag atcatctttt gtcaactcaa ataaatcttt tttaataata gactcagctt
     2521 gacttttgtt ttcagaacga acaatagccg ttacttcatg tcctcgtttg actgcttctt
     2581 caacaattgc cttccccgct tgtccatttg ctgcaataac tgctagtttc attttttata
     2641 cccctcttgt tgtaattatt ttagttacaa caattgtgac actcttaata atcaatgtca
     2701 atagtcttgc ttaattatta tcaaaatatt tctaccaaga aaactaacca tgattctagt
     2761 gaaaagaaaa atcttctttg tcaacaaatt tactttcttg ttttaaacat actataataa
     2821 tcatagcaag agatctaagt tgtctgtttt tttaaaacga ggtgattatc atgcgtagat
     2881 tctattccca tctcccctac tatctggtca tattattctt ttattggcca ctttatgagt
     2941 tgttcttgct agttgtttct gaccccctta cactcaaggg actctatata aacaatcttc
     3001 tcttctttac acctatggta atcttgattg tatcgttact ctatagctac cgtttccgtt
     3061 tctcactttg atggttagtt ggtaacggac tgctctttta ctttactatc ataacctttg
     3121 gtgtgtttat actaatttac ttgctaatct atgaaacagt tgctctggtg ggcatggatt
     3181 ctggtattag catcaagcat attctacaaa aaatgaaaaa caaaaaactt tcacaaaatc
     3241 cttgaaaaat ctcacaatca tgctataata atccatagag acaagtcact tagtcccttt
     3301 ctactagaga gtgcgtggtt gctggaaacg cataggaagt ctaaactgat actactcttg
     3361 agttttttat gaaaacataa aacggtggcc acgttagagc cgatcagagg tgtccctctc
     3421 ttttgaggta cataaatgaa ggtggaacca cgttgcgacg tcctttcgag gatgtcgcat
     3481 ttttttatta ggatactaat tatggagttg caagaattag tggagcgcag ttgggcaatc
     3541 cgacaagctt atcacgaact ggaagttaag catcatgatt tcaagtggac ggtagaagaa
     3601 gacctcttgg ctttatctaa tgatattgga aatttccaat gactggtgat gacaaagcaa
     3661 ggacgctact atgatgaaac accctacaca ctggaacaaa aactttcaga aaatatctgg
     3721 tgactattag aactttctca acgtttggat atagatattc tgacggaaat ggaaaacttc
     3781 ctctctgata aagaaaagca attgaacgtt agaacttgga agtagtctgc tgataaaaaa
     3841 tcaatgctta gaaactatga aataataaaa aaggagaaca tcatgattaa cattactttc
     3901 ccagatggcg ctattcgtga attcgaatct ggcgtaacaa cttttgaaat tgcccaatct
     3961 atcagcaatt ccctagctaa aaaagccttg gctggtaaat tcaacggcaa actcatcgac
     4021 actactcgcg ctatcactga agatggaagc atcgaaattg tgacacctga tcacggagat
     4081 gcccttccaa tcttgcgtca ctcagcagct cacttgttcg cccaagcagc tcgtcgtctt
     4141 ttcccagaca ttcacttggg agttggtcca gccatcgaag atggtttcta ctacgatact
     4201 gacaacacag ctggtcaaat ctctaacgaa gaccttcctc gtatcgaaga agaaatgcaa
     4261 aaaatcgtca aagaaaactt cccatctatt cgtgaagaag tgactaaaga cgaggcacgt
     4321 gaaatcttca aaaatgaccc ttacaagttg gaattgattg aagaacactc agaagacgaa
     4381 ggcggtttga ctatctatcg tcagggtgaa tatgtagacc tctgccgtgg acctcacgtt
     4441 ccatcaacag gtcgtatcca aatcttccac cttctccatg tagctggtgc gtactggcgt
     4501 ggaaacagcg acaacgctat gatgcaacgt atctacggta cagcttggtt tgacaagaaa
     4561 gacttgaaaa actaccttca aatgcgtgaa gaagctaagg aacgtgacca ccgtaaactt
     4621 ggtaaagagc ttgacctctt tatgatttca caagaagtgg gacaaggttt gccattctgg
     4681 ttgccaaatg gtgcgactat ccgtcgtgaa ttggaacgct acatcgtaaa caaagagttg
     4741 gcttctggct accaacacgt ctacactcca ccacttgctt ctgttgagct ttacaagact
     4801 tctggtcact gggatcatta ccaagaagac atgttcccaa ccatggacat gggtgacggg
     4861 gaagaatttg tccttcgtcc aatgaactgt ccgcaccaca tccaagtttt caaacaccat
     4921 gttcactctt accgtgaatt gccaatccgt atcgctgaaa tcggtatgat gcaccgttac
     4981 gaaaaatctg gtgccctcac tggccttcaa cgtgtacgtg aaatgtcact caacgacggt
     5041 cacctattcg ttactccaga acaaatccaa gaagaattcc aacgtgccct tcagttgatt
     5101 atcgatgttt atgaagactt caacttgact gactaccgct tccgcctctc tcttcgtgac
     5161 cctcaagata ctcataagta ctttgataac gatgagatgt gggaaaatgc ccaaaccatg
     5221 cttcgtgcag ctcttgatga aatgggcgtg gactactttg aagccgaagg tgaagcagcc
     5281 ttctacggac caaaattgga tatccagatt aaaactgccc ttggaaaaga agaaaccctt
     5341 tctactatcc aacttgattt cttgttgcca gaacgcttcg acctcaaata catcggagct
     5401 gatggcgaag atcaccgtcc agtcatgatc caccgtgggg ttatctcaac tatggaacgc
     5461 ttcacagcta tcttgattga gaactacaag ggggccttcc caacatggct ggcaccacac
     5521 caagtaaccc tcatcccagt atctaacgaa aaacacgtgg actacgcttg ggaagtagcc
     5581 aagaaactcc gtgaccgcgg tgtccgtgca gacgtagatg agcgcaatga aaaaatgcag
     5641 ttcaagatcc gtgcttcaca aaccagcaag attccttacc aattaattgt tggagacaaa
     5701 gaaatggaag acgaaacagt caacgttcgt cgctacggcc aaaaagaaac acaaactgtc
     5761 tcagttgata attttgttca agctatccta gctgatatcg ccaacaaatc acgcgttgag
     5821 aaataagagt ctagcataaa agcctccaat ctggaggctt tttctcatct atttttactc
     5881 aaggactaag ttcacttgag caaactgaat ccgcactgtc gttccttttc cgacctcaga
     5941 ctcgatacga atctggtgcc ccagttcttc agaaattttc ttagatagat aaaggccaag
     6001 tccagaggac tgctgggtca aacggccatt gtatcctgaa aagccacgtt caaatactcg
     6061 gaggacatca ctgtttttta tcccgattcc cgtatctttg atacaaagct cttggtcatc
     6121 catataaatc tccagaccac cttccttggt gtacttgaga ctgtttgaga tgatttgctc
     6181 aataaccact agcagccact ttttatccgt cacgatttct ttatcaaggt catgtagatt
     6241 gacatttaag cctttttgaa taaagaaaag agcatattta cgaattattt ccttgaccaa
     6301 gtcctcaatt tgaacctgct ttaagagcaa atcatcatgg aaactttcta aacgcaggta
     6361 ctgtaaaact aggttggtat aagagtcgat tttgaaaatt tcctgttcta gctgctgctt
     6421 cagttggcgg tcgaccactt ctgcaactaa gagttgactg gctgcaatgg gggtctttat
     6481 ctgatggacc cacaaggtat agtaatccag caaatccgtc agttttcttt ctgcttttga
     6541 cctctgctga tagagttcca tctcacgcgc ttctaatttt tctgctaaag ctatttccaa
     6601 aggagacttg gcttccctct ctccatagag aagttcctgg cgatagacct gcatttccac
     6661 caatatgtcc caagtgaaaa ataagatggt tacaaagcaa cacaagaaga aaaagtagag
     6721 gaagtaaatt cctagactgg caaataaaaa ctgaaagagt aagacaagaa atgccaaaga
     6781 aagcagatag ataaaaagac gactacggga gcgcagatag gctagaaaaa attgtttcca
     6841 atcaagcatg cttcaatccg taccctattc ctttcttggt ctcgataaat cctaccaatc
     6901 cctgctcctc caacttttta cgcaaacgag ccacattgac agagagggta ttatcatcaa
     6961 tgaaaaagtc actgttccaa agttcccgca tcaggtcgtc acgtgctacg atgttgcctg
     7021 catgctcaaa taacacgcgt aaaatctgga attcattctt ggtcaaattc aagacttgcc
     7081 cttgataatg taaatccatg gatttggtat tgaggataac accagcatat tccagcaaac
     7141 tctcatcacg cccaaactca taggaacgac gcaacaagcc ctgaacctta gctaaaagaa
     7201 cctgctggtc aaaaggcttg gtcacaaagt catccgcccc catattgatt gccatgacaa
     7261 tatccatagc ctggtctctc gaagaaagaa acatgatagg taccttggaa atcttgcgga
     7321 tttcctgaca ccagtgataa ccattaaaca agggcaaacc aatatccatg aggaccagat
     7381 gaggttccga ctgaacaaat agactcaaaa cttccataaa gtcttctacc aggaccactt
     7441 caaatcccca ttcagagagc attttcccaa tctgttgacg aatgacctga tcatcttcta
     7501 ttaataaaat cttgtgcatg cgcttctcct tttccattat tataacagat ttttccatgc
     7561 tagatggtct gaaactgaat ttgaaatagc ctgtttttag tcagtgcaaa caggctatgc
     7621 tactagctaa tttgagggaa atttgctaag ataaataaaa agaaaggagc tcttatggcc
     7681 aatatttttg actatctgaa agatgtcgca tatgattctt attacgacct tcccttgaat
     7741 gagttagaca ttctaacctt aatagaaatc acctacctct cctttgataa tctggtctcc
     7801 acacttcctc aacgtctttt agatctagca cctcaggttc caagagagcc caccatgctt
     7861 actagcaaaa atcgccttca attattagat aaattggctc aacacaagcg cttcaaaaat
     7921 tgcaaactct cccattttat caacgacatc gaccctgaac tgcaaaagca atttgcggct
     7981 atgacttatc gtgtcagcct cgatacctat ctgattgtct ttcgtgggac agatgacagt
     8041 atcattggct ggaaggaaga tttccacctg acctatatga aggaaattcc tgctcaaaag
     8101 cacgcccttc gctatttaaa gaactttttt gcccatcatc ctaagcaaaa ggttattcta
     8161 gctgggcatt ccaagggagg aaatctcgct atctatgctg ctagccaaat tgagcaaagt
     8221 ttgcaaaatc agatcacagc agtttataca tttgatgcac ctggtctcca tcaagaattg
     8281 acacagactg cgggttatca aaggataatg gatagaagca agatattcat tccacaaggt
     8341 tccattatcg gtatgatgct ggaaattcct gctcacaaaa tcatcgttca gagtactgcc
     8401 ctgggtggca tcgcccagca cgataccttt agttggcaga ttgaggacaa gcacttcgtc
     8461 caactggata agaccaacag tgatagccag caagtagaca caacctttaa agaatgggtg
     8521 gccacagtcc ctgacgaaga acttcagctc tacttcgacc tcttctttgg cactattctt
     8581 gaggctggta ttagctctat caatgacttg gcttccttaa aggcgcttga atacattcat
     8641 catctctttg tccaagctca atccctcact ccagaagaaa gagaaacctt gggtcgcctt
     8701 acccagttat tgattgatac tcgttaccag gcatggaaaa atagataata ctcttgaaaa
     8761 ttaaatgtat acaaaacaaa agacctagaa tacatacttt catgtgcatt ctaagtcttt
     8821 ttaaatagaa tctaatagtc aataaaaatc aaagagcatt gagagataat ggggcttgga
     8881 acgtccctct cgcttcaaca aaatgacccc attatagatt aaaaagatgc cacttagaaa
     8941 aagcaaaaaa ggaagtaaga caaaggcaaa tacataaaaa gctaactgaa cattctcgta
     9001 tccattttta taaaaaaggt aggatagata aaaataactt gaaatgaggg ataataaaaa
     9061 taatactgga ttccacaaac ttctattatc cttccaaaat gacactataa aggctaatac
     9121 aattcctata acgagataca tttcttactc ctttaatagc tacattttat cataattatc
     9181 caaagaaaaa agagggcatt tatccctctt aatccttcat ctgactctct gcatcggcca
     9241 cgactttttc tagactggtt tgaccaagtt ctgcctccat agtcaactga attctctcca
     9301 atttttgatc caaaacatca tgaatatgag ctcctacagg gcaatttgga ttcggattgt
     9361 catggaaact gaagagttga cctgtcttac caagacattc gaccgcctga taaacatcta
     9421 aaagactaat atccttaagg tccttgacaa tatctgttcc gcccgttcca cgcgctactg
     9481 aaatcagctc tgccttcttc aactgggaca agatctttct gataatgaca ggattgaccc
     9541 cgacactagc agccagaaaa tcactggtca ccttgctttt cttcccctcg agggcaatga
     9601 ttatcagcat atgagtcgca atggtaaatc tacttggaat ttgcatcctc ttctcctttt
     9661 tacgaggcta ccctgcctct actcttcttt ttctattatt ataccctttt tagttgtaat
     9721 gtcaatcgtt accacttttc aaccagtcgt ctaactcccg atcgcaaccc tctttctgag
     9781 ccaattctct caaaaattcc tgatgatgag tatggtggat cccattgacc agactttcat
     9841 agtaaacctc aaaataggga agtctcaggt ctttagccag ctgcaattca gctgccacat
     9901 cgtaatctac ccgtcggaag tccatatcta ccaggccctt gtcatcaaac tccaaaatca
     9961 tatactgggc ccgcaagtcc ttccgtagct gagcgtccaa aaagaaaggt tggccaatcg
    10021 aacccggatt gacaatcaat tgcccaccag tcccgtaacg aagcaactgc tggtgaatgt
    10081 gtccataaac agcaatatca cagggaggat gagtcaccaa gcggtcaaat tcctcttgtt
    10141 tgccagtatg aatcaactct cgcccccagt tcttatcagg cagatgatgg ctaattccca
    10201 ctgtcaaatc cccaaactga cgatgaattt gaagaggttg attgtggagc acttcaattt
    10261 cttctaggga aatttcctct aaaacatact ggcactggcg caagagatag cgttgactag
    10321 ggcgagtact atccaattcc ttacggacac catgccaaag actgtcttcc cagtttccca
    10381 aaactctagc cgtaatcggt agttgatcca acaagtccaa aatccttcta cgccctgtcc
    10441 ctggcatgag aatatctccc aaaagccagt attcatccac tcctagctgc cgagcatctg
    10501 ccaaaacagc ctccaaggcg gtggtatttc catgaatatc tgaaagaaga gctattttcg
    10561 tcatatccat ctcctcgttt tttctcttgc aataagtata acataaaaag tcacagctag
    10621 agaaatctag ctttttttga tatactagat aaagatatta gacaagagga aacgaatgac
    10681 cccaaacaaa gaagactatc taaaatgtat ttatgaaatt ggcatagacc tgcataagat
    10741 taccaacaag gaaattgcgg ctcgcatgca agtctctccc cctgccgtaa ctgaaatgat
    10801 caaacgaatg aaaagtgaaa atctcatcct aaaggacaag gaatgtggct atctactgac
    10861 tgacctcggt ctcaaactgg tctctgagct ctatcgtaag caccgcttga ttgaagtttt
    10921 tctagttcat catttagact atacaagtga ccagattcac gaggaagctg aggtcttgga
    10981 acacactgtc tctgacctgt tcgtggaaag actagataaa ctgctaggtt tccctaaaac
    11041 ctgcccccac ggaggaacta ttcctgccaa gggagaactc ctcgttgaaa tcaataacct
    11101 cccactagct gatatcaagg aagctggcgc ctaccgcctg actcggatgc acgatagttt
    11161 tgacattctc cattatctgg acaagcactc acttcacatc ggtgaccagc tccaagttaa
    11221 gcagtttgat ggcttcagca ataccttcac tatcctcagt aacgacgagg atttacaagt
    11281 gaatatggac attgcaaaac aactctatgt cgagaaaatc aactaatttc tcaagtcccc
    11341 taccaaccct gaaagtttta ttttcagggt tttatttcta tcattttatt ttactttcat
    11401 ttggtggtag aatatttgaa aaaaagaaag gtagtttaac ctatgaaaaa atcactaaaa
    11461 atttttgcta catctaaata gtttgacctc ttcggggttg ctttggtcgt tggattgcga
    11521 ttgcatctgg ttacctcaac tcacgcctcg ataaatttgt agactgggga ccatggacag
    11581 ctcttgtacc ctttggattg atttccgtaa ccaacgttgg gatttccatg ttgtccactc
    11641 gtttcacggg gaaattaagc aaatgggaaa attactttgg tattgttaat accattttgt
    11701 ccggtgctat tgattatatc cttggaaata aggcggccat cattacctat cccgtcacct
    11761 tcctcattta tacctttgct ataaagaaat ggaaagcttc gcaagaaggc agacccaacc
    11821 aaatgagcca aaaacaggta aaattggcgg ccatcatcat ttccatcatc gccttcctct
    11881 ttgcctttgt gaccaactat atcggatatg ggggcaagat gaatctcctt gcctacgtaa
    11941 caactattgc ctttgcactg tccctcattg ccaatgcttt gaatgcattg gcaatgaggg
    12001 acagtggggc ttttggttga tttacaattt cgttcagctg acaaaagctg gtattcaagg
    12061 aaacttcgcc aatatcggta aatacatctt ttatatcctc aatgcaatcg gagctttatt
    12121 tgtctggaat gatgaagaag ttagataata gaaggaaact caaataaaga atactagcca
    12181 acttaagaga atctcccctc tgctattttt atcattttag ttgtaactcc cattctttta
    12241 gagtagaatg aagttagata aaagagggag gtcttgtcat gaaaaaactc tatagaatcc
    12301 attttatagc aattgcagtt attgatttgc tactatttgc attttttatc actagactcg
    12361 agacatcttt tgagtggctc ttgctctctg gtttaatttt cttccttgct caaggactcc
    12421 tgctatttct cttggtcgtc cgactcaaac atcaattcgc tgagatttat cctcaaatca
    12481 ataaaaagat tcgcttctac tatttagggg ttctcaccat tgattttcta ttttttgttc
    12541 tcttagcctt cattagttct cagcgttttt catctcttat gccaatcatc actgcttgcc
    12601 attctacttt ttattatatg acagctgact acctaagaga aaactatcca gacttttacg
    12661 acaaacacat ctctaacacg tatactattt tatcgatttc ttctttttta gtataagaca
    12721 gctcgttctc cataaatatt cattaacttt catttttata cttttccttt cgattttcaa
    12781 aaacaagggg agggattaac ctcccttata ttagacataa cttttaggat cacaattgta
    12841 tgttcctttc gaaatatccc caaaaatcgt agcccctgtt aaataatgat ttcttgtaga
    12901 agcatatgct attttcttca tcacatccag tctcgctact tttagctcga gattaggatt
    12961 ggaaaacata gcatttttaa tatcttccaa atcttgagaa ctaggtgcga gtagagttac
    13021 atcgccctcc aatctattag ccttgtctat ataaagacta actctttcag cactttctgt
    13081 tcgcagaaaa tttagtacat ttctattcat ttttttacct cgtatttttc taattattga
    13141 ttttttctag aaacaaaatc aattcaacgg tgaagctcta gctttcatct tcattcgtac
    13201 ctccttttgt gtatatctag ctttacataa acctatgatt tagagtatag tttctcaatt
    13261 attctcagaa actatggaga tgatttaatt atcatacaaa tcaaagacct ctccttgctc
    13321 atcttcaccg taactctcga gttcctcata aaagtattcc aaagcagttt gttcttctgg
    13381 tgtcataaag aaactccttt ctaaacaata ttagtaaaaa atacgttctt atttccatcc
    13441 taaataaagt agtgttttta tcccgtaata ggttttattt ttgcggagca ttacactccg
    13501 cattaatatt gatagtatta agaatcatta ttacttaata aaaattgcgc tctcaaacat
    13561 agacgtatac aaaatgccct atgtatacgt ttcgcttgga agacatctaa gaaaaaattt
    13621 tgtagttcag acagtaagat taatacatct ttctctaaca taagtgatgt ctggtactcc
    13681 caattattgc tctgttttaa cgatgaaaaa ggtatagctg tctcttttaa ttttatccaa
    13741 tctatacgtt cttttttagg aacgtattct ctaacagctt gactaacaat actggaatag
    13801 cttacttcat agcctaattt attgtaaaca tcatctttaa gcttatctaa tatctgtctg
    13861 gattcagacg ctaaacgcat tctaacattc tcttttccca tattatcacc cctttctgac
    13921 gggattctgt tatcccgtaa attaagtata taacaattta gaaaaaaaat caagaattaa
    13981 gttgaaaaaa attccacaat ctaaaaattt atcattttat gacaaacata ctatcgttaa
    14041 atgctgattt aatcgaaatt ttgagttata aactcctcag tttcgaactc aaaattagcc
    14101 gtgcaggata ggataccctt cataaactta tataactact ttaatacctt aaaaagctaa
    14161 taaaataagg aatatctaag caatatacat tctaaaatac ctgtacggga gtgtctctaa
    14221 agaaaaggag gttttagcat gaaaaaaatc atcttcatca aaaccattca actccttgtc
    14281 attgatggaa tcatgctggc atttttgaca tttaaaaggg gggcttactt gggactggat
    14341 tttgatttat agtggttggc tcattttctt tcatcctgtg ctattgacct atctttcaaa
    14401 ccaactttgt gaccacttta gtcaactcta ttcccagatt agaccgagat tctggcgttt
    14461 tgctttacaa attctcctat gggatagcct gatgattctc tccttggtgt ctttaagtga
    14521 tattccactt ttccttcagg gaactctcct catcctagga catctcatcc cttcctatcg
    14581 catctgccaa agcctgaaaa gagacttccc ccaagcatat caagaaccga tttctttttg
    14641 gagtatttta tgatcgatga gaaagaccaa gccgactggg cttggtcttt cttatctctt
    14701 tttagtatct aggataatgg taacaggtcc gttattaacc agctcaacct gcatatctgc
    14761 tccaaagata cctgtctgaa cgggcacttc ttgcgctaat ttttgattga aagcatcata
    14821 gaagtctgat gccatatcag gtttagctgc ccctgtaaag gctggacgat tgcctttctt
    14881 agtatccgca aagagggtaa actgagaaat agagaggatt tctccttcaa tatctttgac
    14941 agacaggttc atcttgcctt ctgcgtctga aaaaatccgc atattgacca gttttctcac
    15001 agcatagtcc aaatcttcct cttggtcctc tggtccaaca ccaaccagca ataaaagtcc
    15061 ctgattgatt tttccctgaa tctggccttc tatactcact tgggcttttt taacccgttg
    15121 gataatgatt ttcataatag cctttctagt aagagctagg acaactagcc gttggtccgt
    15181 ttgacagagt aaacttctgg cacactctta attttatcga caaccgtggt cagtgtagag
    15241 aggttggcaa taccgaagga cacatggata ttagcaaact tcatatcctt ggttggttgg
    15301 gcattgaccg ttgaaatatt cttggttgta tttgaaagaa cttgcagtac atcgttcaac
    15361 agtcctgtac ggttgagacc gtagatatcg atatgggcca tatactcctt atttgagcta
    15421 gagtactggt cttcccattc cacatcaagg agacgttgct cgtagttttc ttgggcacgc
    15481 aggttcatac agtccacacg gtgaatagcc acaccacgac ccttggtaat gtagccaaca
    15541 atatcgtcac caggcacggg gttacaacac ttagcaatcc gcactaggag accagaagca
    15601 ccttcaataa ccactccccc ctcatgcttg accttgagag tttctttatt ttcaaccttg
    15661 acctcgccac ctttgacaag ctcctctgcc tcagccttgg ccttggcacg ctcttcctca
    15721 cggcgttcct tttcagtcag acggttaaag acggtaatcg caccgatttc cccaaaacca
    15781 atggccgcaa agagggagtc ttctgtcttg taactggtct tttgcagaac ttgatccatg
    15841 tggcgcttgt ccataaattt atttgccaca tagccatttt cttggaactg agccatcagc
    15901 atctcacgac ccttgttgac agacaattcc ttatcttggt ttttaaagaa ctggcgaatc
    15961 ttattgcgcg ccttgctagt cttgaccata ttgagccagt cacggctagg tccaaaggag
    16021 ttcgggttgg cgataatttc aacctgatcc cctgtcttta acttggttgt cagtggaacc
    16081 atgcggccat tgaccttggc accagttgct ttttcaccga ccttggtatg gatttcgtag
    16141 gcaaaatcaa tcggtcctga atctttggga agagaacgga cagctccatc tggggtaaaa
    16201 acgtaaatct cctcagccag atagttttcc ttaacagagt ccacaaattc cttagcatca
    16261 tcagcctggt cttggagctc catcatctcc ttgatccagt tcattccaat agctgattcc
    16321 ttgctgttaa cttgcccctt tatacctttc ttataagccc agtgagccgc aaccccgtac
    16381 tcagccacct cgtgcatttc cttggttcga atctggaatt caatcggccc ttttggtcca
    16441 taaacagtcg tatggataga ctgataacca ttagccttgc ggttggcaat atagtctttg
    16501 aagcgacctg gcatcggttt ccaaaattca tgcacgtaac caagcatggc ataaacatca
    16561 ctttgggtat ctaaaataca acgaatagca atcagatcat agatttcctc aaaccgtttt
    16621 ctcttgtcct gcattttgcg gaaaattgag taaatatgct tgggacgacc ataaatcttc
    16681 cctttcaagt gacgttctgt cgtatactcc tctaattttg tgactacctc atccaccaag
    16741 gcctcacgct ccctgcgctt ttccttcatc atatgggtaa tcttgtaaaa ctccgttgga
    16801 ttgagataac ggaaagacaa gtcttctaat tcccatttga cactggaaat ccccaaacga
    16861 tgggcaagcg gggcatagat ttccatggtt tctttggaaa tacgctcctg cttgtctttt
    16921 cgaagatgtt tcagggtccg catattgtgc aagcggtcag acagtttgac caaaataacg
    16981 cggatgtcct cagacatggc catgagcatc ttgcgatgat tttccgctaa ttgctcctcg
    17041 atcgatttgt actcgacctt gccaagcttg gtaactccgt caacaatcat ccgcacatca
    17101 ggaccaaact ctctttccaa atcgtccaaa gtcgcatctg tatcttccac cacatcatgc
    17161 aagaatccac aagctactgt tacagcatcc agctttagct tagctaaaat acctgccact
    17221 tggatagggt gaatgatata aggctcgcct gatttgcgat attgaccact gtggcattca
    17281 acagcataga ccaaggcctt atggacaaaa tgaacatcct cttccgttaa atattctttg
    17341 gttaaagcga caacttcttc gcctgttaaa ttcacttctt tcggcatctc tactctccaa
    17401 ttcttcctac cattttatca cttttttaag aatatgaaaa ctagattgga acagaataag
    17461 aaaaaaataa ttcaaaattg cttgataatt ctgaattatt ggcccataat atactacgaa
    17521 gttagatttt aaacttaggt gatagaagga gagatagaag aacggaaacc atattgtaac
    17581 ccaaagactt tctgatttcc ccaattccat tgaagatacg aaagataaac ggtggaactc
    17641 gtatcacata cactggtacc ttgactggat tttggaatta atactcaatg aaaatcaaag
    17701 agcaaactag gaaactagcc acaggttgct caaagcactg ctttgaggtt gtagataaga
    17761 ctgacaaagt cagtcacata tatacggcaa ggcgacgttg acgcggtttg aagagatttt
    17821 tgaagagtat aaaaatcctc aagatacttt cttctatcct ttagtttata aggagaatac
    17881 ctatgaaaaa actgctattt ctatctttgc tctcctaatg ttaggagttt gctgcctgtt
    17941 cctattcagc cagcaaagct ataaaaaact cgttcaatac tatgctaacg accagaacct
    18001 gcccagtagg ataacttata gtgaatatag cgacaaatga gaagccaact acggtagcac
    18061 tctaaacatc acgtctatca aacaagctaa tgacggagtt tatgcaacct atgaagggca
    18121 attgacacct ttccaatatt gataaattga taaccagcct gtcttcatct agtcatgctg
    18181 gtttttaagt tcattttaaa tccttaccta ttctccctaa ctgtgctata cttaatttat
    18241 actcaatgaa aatcaaagag caaactagaa agctagccgc aggctgttca aagcactgct
    18301 ttgaggttgc agataaagtt gacgcggttt gaagagattt tcgaagagta ttagtacatt
    18361 ctttgagatt ggagttagta tgaaaatcca taaaaccgtg aatcctgttg cctatgaaaa
    18421 tacctattat ctagaaggcg aaaagcacct catcgtcgtc gatcctggta gtcattggga
    18481 agccattcgt cagacaatcg agaagatcaa caaaccgatc tgtgctattc tcttgaccca
    18541 cgcccattat gaccatatca tgagtctgga cttggttcgc gagacgtttg gcaatcctcc
    18601 tgtctatatc gcagagagcg aagccagctg gctctacact cctgtcgata atctctccgg
    18661 tctccctcgc cacgatgata tggcagatgt ggtcacaaaa cctgcagaac acacctttgt
    18721 ctttcacgaa gaataccaac tagaggaatt tcgttttaag gttctaccga ccccagggca
    18781 ctctatcggt ggtgtttccc tagtctttcc tgatgctcac ctagtcttga cgggagatgc
    18841 tctattccgc gaaactatcg gacggaccga ccttccgact ggtagcatgg agcaactcct
    18901 tcatagtatc cagacccaac tcttcaccct accaaactac gatgtctatc caggacatgg
    18961 tccagctact actatcgctc acgaaaaggc cttcaatccc tttttctagc aagatgatga
    19021 caatcgaaat ttaagtaaac tatccagcaa atctttctat tacaaaaggc atcctatcaa
    19081 ggttttcaca cgtgattgga tgcctttttt ctgatgacta gattttttgt attaccaaat
    19141 aatcacgcgc tcctctggtg aacgccacat tccgtctcct tctttgacat cataggttgt
    19201 aaagaaatcg tcgaagtttg gtacttgcac attgacacgg agtttggctg gtgcgtgcac
    19261 atcgacgcta gccaaaagtt tcataaattc tggtcgacct ttcatgcgcc agatgcgacc
    19321 gaagttgtag aagaactctt ctgctgagaa gtctgcttct ctcttagctg cttcaagcgc
    19381 tgctgcgatt cctcccaagt cagccacgtt ttctgataca gtcaatttac cgttaatggt
    19441 tgctccataa gaatcctgtc catcaaattg gtcaatgact ttttgtgttt tctccttgaa
    19501 ggcagcatag tcgctctctg tccaccaatc cttgaggcta ccattttcgt caaaggaagc
    19561 cccgttagta tcaaaggcgt gggaaatttc atgggcaatc actgccccaa taccaccgta
    19621 gttagcagaa gatgactgat gcaagtcata gaaaggcgcc tgtaaaatgg ccgctggaaa
    19681 gacaatcagg ttcttctgag gattgtagta ggcattgacc atatgagcag gcatgcccca
    19741 ttccttatag tctacaggct ggttccactt actccaactg tgcttgattt ccacacgcgc
    19801 aaaggctaga gcattctcaa aaagactggc agtttcattc actaccttat ccttgtaacg
    19861 tgcaggcaat tcttctggat agccaatata aggtttgatc acattgagct tcacgatagc
    19921 ctgtttacag gtttctggag tgagccagtc attcttaagc agacgctcct tatagacatc
    19981 aatcatggtt gccacttttt tctccacatc cgccttggct tctggagaga acttctcacg
    20041 ggcgtaccaa agccccaggg cttgcttgaa aggttcctgt gctagatgat aagctgcttt
    20101 gaccttatct tttgcctctg gaactccaga aagggcacgg ctgtaggcac cagacaaaac
    20161 acggatatcc tctgttaaat agctggttga aagattgaca acactcaaaa tcaaggttgc
    20221 tttaaggaga gaccaggctt cctcactgta aaattgctct gctgcttgcc agaaacgttc
    20281 ctcgtctaca ataaccttgt ctggtaattg cccaataact gctttgaaga agtcatccaa
    20341 aggtagggca ggcgcgaatt tcttgaaatc ttcgtaagaa tatggatgat agagtttagc
    20401 atattctgaa ctttcttcat tagagagcac cactgccgca actcggcggt ccaattcaag
    20461 tcttttttct agcaagtctt caatttcttc atcagagaaa tcataagcct tgaggagatt
    20521 tgcgctgctt tctttccaaa gagtcaagag ctcttcgcgc tgaggatgtt cttctgcata
    20581 gtaggtcgta tctggcaaga ttgtgcttgg agcgctagcc catagaacat tgattctagc
    20641 atccataaag tctggcgata caccaaaagg aaggaagttt ggttttcctg caagctcaaa
    20701 ctctgctagt ttagctgtaa aatccgcaaa agtttccaat tcttggaatt ctttaaggag
    20761 tggtaagaca ggtgtgatac cgtcagcttc tctcttgtca aaatcacgaa ctaggcggtg
    20821 gtatttgaca aagttttcca agatagcatc ctcaggcact tcttcacctg ctaaccactt
    20881 gtctgttgtc gccagcatca ggtcttcaat ttcctggtct aaatcaacaa aacctcctgt
    20941 ttgagactta tctgctggga tttcagctgt ctgttgccat tctccattga tagcatcata
    21001 aaaatcatct tgataacgtg tcatcttgtt ctcgctttca tttgtatttg catttatctt
    21061 aacaaaaatc actagggaat gcaattaata accatttaac tattattttt tagctattat
    21121 aaaaaacctt tccatgataa aacgcataat tccaagtttt ttacacttga tactatgcgt
    21181 tttataattt agaaatttat cctgaaagtt atctttagaa tctattttt
//
LOCUS       DAVBCR010000030        20937 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCR010000030 DAVBCR010000000
VERSION     DAVBCR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.030"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(41..961)
                     /gene="cysK"
                     /locus_tag="VQ508_001077"
     CDS             complement(41..961)
                     /gene="cysK"
                     /locus_tag="VQ508_001077"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2490083.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1246..1881)
                     /locus_tag="VQ508_001078"
     CDS             complement(1246..1881)
                     /locus_tag="VQ508_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2490084.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITTIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            1937..3235
                     /locus_tag="VQ508_001079"
     CDS             1937..3235
                     /locus_tag="VQ508_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2490085.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3232..3894
                     /locus_tag="VQ508_001080"
     CDS             3232..3894
                     /locus_tag="VQ508_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2490086.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            3974..4522
                     /gene="raiA"
                     /locus_tag="VQ508_001081"
     CDS             3974..4522
                     /gene="raiA"
                     /locus_tag="VQ508_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2490087.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4658..6637
                     /locus_tag="VQ508_001082"
     CDS             4658..6637
                     /locus_tag="VQ508_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2490088.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDV
                     SREQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMF
                     SRRVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDE
                     IGKVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISD
                     KIRSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFI
                     EKEGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQD
                     FPDNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRV
                     TSRTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTK
                     CYDTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGH
                     FNLAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6634..7086
                     /gene="rplI"
                     /locus_tag="VQ508_001083"
     CDS             6634..7086
                     /gene="rplI"
                     /locus_tag="VQ508_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2490089.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7130..8482
                     /gene="dnaB"
                     /locus_tag="VQ508_001084"
     CDS             7130..8482
                     /gene="dnaB"
                     /locus_tag="VQ508_001084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2490090.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8484..8750
                     /locus_tag="VQ508_001085"
     CDS             8484..8750
                     /locus_tag="VQ508_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490091.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8839..10185
                     /gene="cbpD"
                     /locus_tag="VQ508_001086"
     CDS             8839..10185
                     /gene="cbpD"
                     /locus_tag="VQ508_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2490092.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10268..10370
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10441..10731
                     /locus_tag="VQ508_001087"
     CDS             10441..10731
                     /locus_tag="VQ508_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2490093.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10694..11452
                     /locus_tag="VQ508_001088"
     CDS             10694..11452
                     /locus_tag="VQ508_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490094.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11490..12497
                     /locus_tag="VQ508_001089"
     CDS             11490..12497
                     /locus_tag="VQ508_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2490095.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12497..13225
                     /locus_tag="VQ508_001090"
     CDS             12497..13225
                     /locus_tag="VQ508_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490096.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13344..13802
                     /locus_tag="VQ508_001091"
     CDS             13344..13802
                     /locus_tag="VQ508_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2490097.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            13804..16236
                     /locus_tag="VQ508_001092"
     CDS             13804..16236
                     /locus_tag="VQ508_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2490098.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16878..17531
                     /gene="rr06"
                     /locus_tag="VQ508_001093"
     CDS             16878..17531
                     /gene="rr06"
                     /locus_tag="VQ508_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2490099.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17528..18868
                     /gene="hk06"
                     /locus_tag="VQ508_001094"
     CDS             17528..18868
                     /gene="hk06"
                     /locus_tag="VQ508_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2490100.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18951..19478
                     /locus_tag="VQ508_001095"
     CDS             18951..19478
                     /locus_tag="VQ508_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2490101.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19557..>20937
                     /locus_tag="VQ508_001096"
     CDS             19557..>20937
                     /locus_tag="VQ508_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2490102.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENP
                     APKPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQ
                     PSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NANGSMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQYNGSWY
                     YLNANGDMAT"
BASE COUNT         6572 a         3467 c         4747 g         6151 t
ORIGIN      
        1 ctagtctgga gatttttcaa tagacttcgt tattggacgg ttacaattca taaagtgctg
       61 tagagagata acgttcaccg ttatctggtg ctagggcaag gactttttta cctgtaccta
      121 attttttggc aacctcgatg gctccgtaga tagctgcagc tgaggaaatc cctacaagga
      181 agccttcttt tccaccaatt tcacgtccga gtgcaagagc gtcatctgat gttacacgaa
      241 cgataccatc ataggcttta gtatcaagtg tatcaggaat aaatccagct gagatacctt
      301 gaattttgtg aggaccaggt ttttcaccag atagaatagc agattcatct gcttctactg
      361 caaaaacttg aatgttagaa ttttctgatt tgagtgcatg agaaacacca gaaatcgttc
      421 caccagtacc tactccagca acaaaggcat ctaatccatc tttaccgaaa gcagctagta
      481 tctcagctcc tgttgttctt tcgtgtactt ctggattagc tggattgtca aattgaagag
      541 gaaggaaacc atcacgttca gcagcgattt cttgagcctt agcaatagca cctttcattc
      601 cctcgctacc aggagttagg acgagttcag caccataagc ttggataatt ttacgtcgtt
      661 ctacactcat agtttcaggc ataacgatga cgactttata ccctttagca gcacctaccc
      721 atgaaagtcc aataccggtg tttccacttg ttgcttcaac aatagtagaa ccaagtttca
      781 gaataccatc ttgttcagct ttttcaatca tgctaagggc aatacggtct tttacagatg
      841 aaccaggatt aaatgcttca agctttatat agacgtctgc agcaccttct ggcacgatgt
      901 tgttaagttt aacaatcggt gtttgaccga ttaattcagt aatgttgtta taaatagaca
      961 tatgaatacc tctttgtata agatatcttt agtataacgc tatccatacc atttgtaaaa
     1021 tatagaaatc ctatcgaagc gataggattt ctatatatca tagatcaaag tcattaattt
     1081 tcaagtgatt gcgttcagtt aattctagta aataggtgtg aaggaaaacg atatcaggtt
     1141 tcgtttgaat tgcttcattt tctagtatat tttggtacgc atctttttat tttgctcgct
     1201 aaagtagttg tgagaatgtc aacgaaacgc tgtattattc attgtttaca ctaagtttac
     1261 aggaacttca acctctcgta aaccttggtc agttaaagtg acttttccat taaaaaactc
     1321 cacaagtgca gctttaatag tttctttttc ttctttatca acataaatca tcgtatcaac
     1381 ttgatctgta aagtttgtat ccagctccat gagatcatgt tctttaagga agttactgta
     1441 ctcttggtac tgagcataag acatttgaat agcaatgcca gcctgttctt ttatttcaat
     1501 aataccaatt tctttgacag ctaaggcgac actgccggcg taagcacgaa ttagtcctcc
     1561 agcgcctagt ttaataccac caaagtagcg tgtcacgacc acacagacat tggtgagatt
     1621 gtgattttct agtaccccaa gcatgggaac accagcagta ccactaggct caccatcatc
     1681 acttgtacgt ttaatttcac tacgttctcc aataataaag gcagagcagt tatgtgtcgc
     1741 tttgtagtgt tcttttttga tggtagtaat gaagtcacga gcctcttctt cgctataaac
     1801 acgcttggca tggcagataa agcgagattt tttgatttct tcttggactt ggccgtcctc
     1861 tttaattgtc ctaaattcca tgatttaatt gtactaaaaa atcatctaaa gtgctagttt
     1921 ttacgaataa agaagtatga aagtaaattt agattatctc ggtcgtttat ttactgagaa
     1981 tgaattaaca gaagaagaac gtcagttggc ggagaaactt ccagcaatga gaaaggagaa
     2041 ggggaaactt ttctgtcaac gttgtaatag tactattcta gaagaatggt atttgcccat
     2101 cggtgcttac tattgtcgag agtgcttgct gatgaagcga gtcagaagtg atcaaacttt
     2161 atactatttt ccgcaggagg attttccgaa gcaagatgtt ctcaaatggc gcggccaatt
     2221 aactcctttt caagagaagg tgtcagaggg actgcttcaa gcagtagaca agcaaaagcc
     2281 aaccttagtt catgcggtaa caggagctgg aaagacagaa atgatttatc aagtagtggc
     2341 taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag atgtttgttt
     2401 ggagctgtac aagcgcctgc aacaggattt ttcttgcggg atagctttgc tacatggaga
     2461 atcggaacct tattttcgaa caccactagt tgttgcaaca acccatcagt tattgaagtt
     2521 ttatcaagct tttgatttgc tgatagtgga tgaagtagat gcttttcctt atgttgataa
     2581 tccaatgctt taccacgctg tcaagaatag tgtaaaggag aatggattga gaatcttttt
     2641 aacagcgact tcgaccaatg agttagataa aaaggtccgt ttaggagaac taaaaagact
     2701 gaatttaccg agacggtttc atggaaatcc gttgattatt ccaaaaccaa tttggttatc
     2761 ggattttaat cgctacttag acaagaatcg tttgtcacca aagttaaagt cctatattga
     2821 gaagcagaga aagacagctt atccgttact catttttgct tcagaaatta agaaagggga
     2881 gcagttagaa gaaatcttac aggagcaatt tccaaatgag aaaattggct ttgtatcttc
     2941 tgtaacagag gatcgattag agcaagtaca agcttttcga gatggagaac tgacaatact
     3001 tatcagtacg acaatcttgg agcgtggagt taccttccct tgtgtggatg ttttcgtagt
     3061 agaagccaat catcgtttgt ttaccaagtc tagtttgatt cagattggtg gacgagttgg
     3121 acgaagcatg gatagaccga caggagattt gcttttcttc catgatgggt taaatgcttc
     3181 aatcaagaag gcgattaagg aaattcagat gatgaataag gaggctggtc tatgaagtgc
     3241 ttgttatgtg ggcagactat gaagactgtt ttaactttta gtagtctctt acttctgagg
     3301 aatgatgact cttgtctttg ttcagactgt gattctactt ttgaaagaat tggggaagag
     3361 aactgtccaa attgtatgaa aacagagttg tcaacaaagt gtcaagattg tcaattttgg
     3421 tgtaaagaag gagttggagt cagtcataga gcgattttta cttacaatca agctatgaag
     3481 gattttttca gtcggtataa gtttgatgga gacttcctgt taagaaaagt tttcgcttca
     3541 tttttaagtg aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt
     3601 cctgatagat atgctaatag aggatttaat caggttgagg gcttggtaga ggcagcaggc
     3661 tttgagtatc tggatttatt agagaaaaga gaagagagag ccagttcttc taaaaatcgt
     3721 tcagagcgct tggggacaga acttcctttc tttattaaaa gtggagtcac tattcctaaa
     3781 aaaatcctac ttatagatga tatctatact acaggagcaa ctataaatcg tgttaagaaa
     3841 ctgttggaag aagctggtgc taaggatgta aaaacatttt cccttgtaag atgaggaaaa
     3901 aatttgcaaa aatagaatga aagcgttata ataaaatcat aaaaacaaaa atgtttagaa
     3961 agaaggtact catatgatta aatatagtat ccgtggtgaa aacctagaag taacagaagc
     4021 aattcgtgat tatgtagttt ctaaactcga aaagatcgaa aagtacttcc aaccagaaca
     4081 agagttggat gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt
     4141 aacgattccg cttggatcta ttactctccg cgcagaagat gtatctcaag atatgtatgg
     4201 ttcaattgac cttgtaactg ataaaattga acgtcagatt cgtaaaaata aaacaaaaat
     4261 cgagcgtaaa aataaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga
     4321 agattcaaat gttgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat
     4381 ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt
     4441 ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt
     4501 gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa
     4561 ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt cgctttttga
     4621 ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc
     4681 aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt
     4741 tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt
     4801 tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt
     4861 aaaccaccaa gcggaagaaa gtttgacatc tctattggaa caaatgcctg taggtgttat
     4921 gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt
     4981 gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagc
     5041 ttcagtagga aatccgtcta cttatgccaa gcttggtgag aagcgttatg ctgttcatat
     5101 ggatgcttct tccggtgttt tgtattttgt agatgtatcc agggaacaag ccataacaga
     5161 tgaattggta acaagtagac cagtgattgg gattgtctct gtggataatt atgatgattt
     5221 ggaggatgca acttctgagt cagatattag tcaaatcaat agttttgtag ctaattttat
     5281 atcagagttt tcagaaaaat acatgatgtt ttctcgtcgg gtaagtatgg atcgatttta
     5341 tctatttact gactacacgg tgcttgaggg cttgatgaat gataaatttt ctgttattga
     5401 tgctttcaga gaagagtcga aacagagaca gttgcccttg accttaagta tgggattttc
     5461 ttatggcgat ggaaatcatg atgagatagg gaaagttgct ttgctcaatt tgaacttggc
     5521 tgaagtacgt ggtggcgatc aggtggttgt taaggaaaac gacgaaacga aaaatccagt
     5581 ttattttggt ggtgggtctg ctgcttcaat caagcgtaca cggactcgta cgcgcgctat
     5641 gatgacagct atttcagata agattcggag tgtagatcag gtttttgtag tcggtcacaa
     5701 aaatttagac atggatgctt tgggctctgc tgtaggtatg cagttgttcg ccagcaatgt
     5761 gattgaaaat agctatgctc tttatgatga agaacaaatg tctccagata ttgaacgagc
     5821 tgtttcattc atagaaaaag aaggagttac gaagttgttg tctgttaagg atgcaatggg
     5881 gatggtgacc aatcgttctt tgttgattct cgtagaccat tcaaagacag ccttaacatt
     5941 atcaaaagaa ttttatgatt tatttaccca aaccattgtt attgaccacc atagaaggga
     6001 tcaggatttt ccagataata cggtcattac ttatatcgaa agtggtgcaa gtagtgccag
     6061 tgagttggta acggaattga ttcagttcca gaattctaag aaaaatcgtt tgagtcgtat
     6121 gcaagcaagt gtcttgatgg ctggtatgat gttggatact aaaaatttca cctcgcgagt
     6181 aactagtcgg acatttgatg ttgctagcta tctcagaacg cgcggaagtg atagtattgc
     6241 tatccaggaa atcgctgcga cagattttga agaatatcgt gaggtcaatg aacttatttt
     6301 acaggggcgt aaattaggtt cagatgtact aatagcagag gctaaggaca cgaaatgcta
     6361 tgatacagtt gttattagta aggcagcaga tgccatgtta gccatgtcag gtattgaagc
     6421 gagttttgtt cttgcgaaga atacacaagg atttatctct atctcagctc gaagtcgtag
     6481 taaactgaat gtacaacgga ttatggaaga gttaggcggt ggaggccact ttaatttggc
     6541 agcagctcaa attaaagatg taaccttgtc agaagcaggt gaaaaactga cagaaattgt
     6601 attaaatgaa ataaaggaaa aggagaaaga agaatgaaag taatcttttt agcagatgtt
     6661 aaaggaaaag gtaaaaaagg cgaaattaag gaagtaccaa cagggtatgc gcaaaacttt
     6721 cttatcaaaa agaatctagc caaagaagcg actgctcaag ctgtaggtga acttcgtggt
     6781 aaacaaaaat cggaagaaaa agctcacgct gagatgattg cagaaggaaa agcaattaaa
     6841 gcacaacttg aagcagaaga aactgttgta gaatttgttg aaaaagttgg tccagatggt
     6901 cgtacctttg gctctattac caataagaag attgcagaag aattgcaaaa acaatttgga
     6961 attaagattg ataaacgtca tattcaagta caagctccga ttcgagcggt tggtttgatt
     7021 gatgtgccag tgaaaatcta tcaagatatc acaagtgtaa tcaatcttcg tgtgaaagaa
     7081 ggataagttt acaccttctt gacaagattg taaaaggaag ggaagtctga tggcagaagt
     7141 agaagagtta cgagtacaac ctcaagatat cttagctgag caatccgttt taggggctat
     7201 ctttattgat gagagtaaac ttgtttttgt gcgagaatac attgagtctc gggacttttt
     7261 taagtatgcc catcgtttga ttttccaagc catggtcgat ttatccgatc gtggtgatgc
     7321 catagatgca acaacggttc gtactatcct tgataatcaa ggtgatttac agaatattgg
     7381 tggcttgtct tacttggttg agattgttaa ttctgtgcca acttctgcta atgcggagta
     7441 ttatgctaag attgttgcag aaaaagcaat gctacgtcgt ttaattgcca agttgacaga
     7501 gtctgtcaac caagcttacg aagcgtcaca accagctgat gaaattattg ctcaggcaga
     7561 aaaagggctg attgatgtca gtgaaaatgc aaatcgaagc gggtttaaga acattcgaga
     7621 tgtgttgaat ctcaactttg gaaatctgga agctcgctcg caacaaacga ccgatattac
     7681 aggtattgcg acaggttatc gtgatttgga tcatatgaca acaggacttc atgaggagga
     7741 gttgattatc ttagcagctc gtccagcagt tggtaagaca gcatttgcct tgaatatcgc
     7801 tcagaacatt gggactaagt tggacaaaac ggttgctatt ttttcactcg aaatgggtgc
     7861 ggaaagcttg gtagatcgta tgttagctgc agaaggctta gtggagtcac attctatccg
     7921 tacagggcaa ttgacagatg aggagtggca aaaatatact attgctcagg gtaatctagc
     7981 taacgccagt atctatatcg atgatacgcc aggtattcgg attacagaga ttcgttctcg
     8041 ttctcgtaaa ttggctcaag aaactggaaa tcttggtttg attttgatag actatttgca
     8101 acttatcacg ggaactggtc gagaaaatcg tcaacaagaa gtttctgaaa tttctcgtca
     8161 gttgaaaata ctagccaagg aattgaaggt tccagtaatc gctctgagtc agctttctcg
     8221 tggtgtagaa caacgtcagg acaagagacc ggtcttgtct gatattcgtg aatctgggtc
     8281 tattgagcag gacgctgata tcgtagcttt tctctatcgc gatgactact atgaacgtgg
     8341 tggtgaagaa gaggagggta tcccaaataa taaggtggaa gttattatcg agaaaaaccg
     8401 tagtggagct cgtggaacag tggaattgat tttccaaaaa gaatacaata aattttcaag
     8461 tatctcaaag agggaggcat aagatgtcag atgcatttac agatgtagcc aagatgaaaa
     8521 aaatcaaaga agaaatcaag gcacatgagg gacaagtcgt agaaatgact ttggagaatg
     8581 gtcgtaagcg ccaaaaaaat agattgggta agctaattga agtttatcca tctctattta
     8641 ttgtggagtt tggggatgtg gaaggagata aacaagttaa tgtttacgtt gaatccttta
     8701 cttactcaga tattcttaca gaaaagaatt tgattcatta tcttgactaa agtgagaaat
     8761 tttctcactt tttctttttt ctccgaataa tttaggtgaa ggcaatcatc gctttatatt
     8821 atttttcaag gaggaagaat gaaaatttta ccgtttatag caagaggaac aagttattac
     8881 ttgaagatgt cagttaaaaa gcttgttcct tttttagtag taggattgat gctagcagct
     8941 ggtgatagtg tctatgccta ttccagagga aatggatcga ttgcgcgtgg ggatgattat
     9001 cctgcttatt ataaaaatgg gagccaggag attgatcagt ggcgcatgta ttctcgtcag
     9061 tgtacttctt ttgtagcctt tcgtttgagt aatgtcaatg gttttgaaat tccggcagct
     9121 tatggaaatg cgaatgaatg gggacatcgt gctcgtcggg aaggttatcg tgtagataat
     9181 acaccgacga ttggttccat tacttggtct actgcaggaa cttatggtca tgttgcctgg
     9241 gtgtcaaatg taatgggaga tcagattgag attgaggaat ataactatgg ttatacagaa
     9301 tcctataata aacgagttat aaaagcaaac acgatgacag gatttattca ttttaaagat
     9361 ttggatggtg gcagtgttgg gaatagtcaa tcctcaactt caacaggcgg aactcattat
     9421 tttaagacca agtctgctat taaaactgaa cccctagtta gtgcaactgt gattgattac
     9481 tattatcctg gagagaaggt tcattatgat caaattctcg aaaaagacgg ctacaagtgg
     9541 ttgagttata ctgcctataa tggaagctat cgttatgttc aattggaggc tgtgaataaa
     9601 aatcctctag gtaattctgt tctttcttca acaggtggaa ctcattattt taagaccaag
     9661 tctgctatca aaactgaacc cctagttagc ggaactgtga ttgattacta ttatcctggg
     9721 gagaaggttc attatgatca gatacttgaa aaagacggct acaagtggtt gagttatact
     9781 gcctataatg gaagtcgtcg ctatatacag ctagagggag tgacttcttc acaaaattat
     9841 cagaatcaat caggaaatat ctctagctat ggatccaata atagttcaac tgtcggttgg
     9901 aagaaaataa atggtagttg gtatcatttc aaatcaaatg gttctaaatc aacaggatgg
     9961 ctgaaagacg gttctagctg gtattatttg aaattatctg gtgaaatgca gacaggatgg
    10021 ttaaaggaga atggctcgtg gtattatctg ggtagttcag gggcaatgaa aacaggctgg
    10081 taccaggtct ctggtaagtg gtattattct tactcttcag gcgccttagc tgttaatacg
    10141 acggtggatg gctacagagt aaacagtgat ggagaacgag tatagaaaat tggagtagga
    10201 gaaatttcct gctcttttct gtaagcagtt tccttgacat ttttcttatt ttgcgctatc
    10261 atatatacat ataatatatg ggagtctgtg tacagtctga gaggaagtgt taaacttcga
    10321 ccgcacctga tctgggtaat gccagcggag ggaacgatac ttagtctaat tttgcacctt
    10381 ttccatgtat ggtaaaggtt tttctttttt ttaaaaggaa aacgagaaga ggaggttctt
    10441 atgaaagcaa gcattgcctt gcaagtttta cccctagcac aggggattga tcggatagct
    10501 gttattgatc aggtcattgc ttatctgcaa actcaagaag tgacgatggt agtgacacca
    10561 tttgaaacgg tcttggaagg ggagtttgat gagcttatgc gcattctaaa agaagcgctg
    10621 gaagtggcag ggcaggaggc agacaatgtc tttgccaatg tcaaaataaa tgtaggagag
    10681 attttaagta ttgatgagaa acttgaaaag tatactgaga cgacacatta gtctattggg
    10741 ctttctcgga gtattgtcaa tctggcagtt agcaggtttt cttaaacttc tccccaagtt
    10801 tatcctgccg acacctcttg aaattctcca gccctttgtt cgtgacagag aatttctctg
    10861 gcaccatagc tgggcgacct tgagagtggc tttactgggg ctgattttgg gagttttgat
    10921 tgcctgtctt atggctgtgc tcatggatag tttgacttgg ctcaatgacc tgatttaccc
    10981 tatgatggtg gtcattcaga ccattccgac cattgccata gctcctatcc tggtcttgtg
    11041 gctgggttat gggattttgc ccaagattgt cttgattatc ttaacaacaa cctttcccat
    11101 catcgttagt attttggacg gttttaggca ttgtgacaag gatatgctga ctttgtttag
    11161 tctgatgcga gcaaaccctt ggcaaatcct gtggcatttt aaaatcccgg ttagcctgcc
    11221 ttacttttat gcaggtctga gggtcagtgt ctcctacgcc tttatcacaa ctgtggtatc
    11281 tgagtggttg ggaggttttg aaggtcttgg tgtttatatg attcagtcta aaaaactgtt
    11341 tcagtatgat accatgtttg ccattattat tctggtgtcg attatcagtc ttttgggtat
    11401 gaagctggtc gatatcagtg aaaaatatgt gattaaatgg aaacgtactt aaaatagagc
    11461 gtttttgaaa aagaaaagag gaaatcaaaa tgaagaaaac atggaaagtg tttttaacgc
    11521 ttgtaacagc tcttgtagct gttgtgcttg tggcctgtgg tcaaggaact gcttctaaag
    11581 acaacaaaga ggcagaactt aagaaggttg actttatcct agactggaca ccaaatacca
    11641 accacacagg gctttatgtt gccaaggaaa aaggttattt caaagaagct ggagtggatg
    11701 ttgatttgaa attgccacca gaagaaagtt cttctgactt ggttatcaac ggaaaggcac
    11761 catttgcagt gtatttccaa gactacatgg ctaagaaatt ggaaaaagga gcaggaatca
    11821 ctgccgttgc agctattgtt gaacacaata catcaggaat catctctcgt aaatctgata
    11881 atgtaagcag tccaaaagac ttggttggta agaaatatgg gacatggaat gacccaactg
    11941 aacttgctat gttgaaaacc ttggtagaat ctcaaggtgg agactttgag aaggttgaaa
    12001 aagtaccaaa taacgactca aactcaatca caccgattgc caatggcgtc tttgatactg
    12061 cttggattta ctacggttgg gatggtatcc ttgctaaatc tcaaggtgta gatgctaact
    12121 tcatgtactt gaaagactat gtcaaggagt ttgactacta ttcaccagtt atcatcgcaa
    12181 acaacgacta tctgaaagat aacaaagaag aagctcgcaa agtcatccaa gccatcaaaa
    12241 aaggctacca atatgccatg gaacatccag aagaagctgc agatattctc atcaagaatg
    12301 cacctgaact caaggaaaaa cgtgactttg tcatcgaatc tcaaaaatac ttgtcaaaag
    12361 aatacgcaag cgacaaggaa aaatggggtc aatttgacgc agctcgctgg aatgctttct
    12421 ataaatggga taaagaaaat ggtatcctta aagaagactt gacagacaaa ggcttcacca
    12481 acgaatttgt gaaataatga cagaaattag actagagcat gtcagttatg cctatggtca
    12541 ggagaggatt ttagaggata tcaacctaca ggtgacttca ggcgaagtgg tttccattct
    12601 aggcccaagt ggtgttggaa agaccaccct ctttaatcta atcgctggga ttttagaagt
    12661 tcagtcaggg agaattgtcc ttgatggtga agaaaatccc aaggggcgcg tgagttatat
    12721 gttgcaaaag gatctgctct tggagcacaa gacggtgctt ggaaatatca ttctgcccct
    12781 cttgattcaa aaggtggata aggcagaagc tatttcccga gcggataaaa ttcttgcgac
    12841 cttccagctg acagctgtaa gagacaagta tcctcatgaa cttagcggtg ggatgcgcca
    12901 gcgtgtagcc ttactccgga cctacctttt tgggcacaag ctctttctct tagatgaggc
    12961 ctttagcgcc ttggatgaga tgacaaagat ggaactccac gcttggtatc ttgagattca
    13021 caaacagttg cagctaacaa ccctgatcat cacgcatagt attgaggagg ccctcaatct
    13081 cagcgaccgt atctatatct tgaaaaatcg ccctgggcag attgtttcag aaattaaact
    13141 agattggtct gaagatgagg acaaggaagt ccaaaagatt gcctacaaac gtcaaatctt
    13201 ggcagaatta ggcttagata agtagaaaaa tagggagttg gtgaagatta tcctttacca
    13261 gcgccctttt tcttttaaaa atgagaaaat ttcggtataa tagtcaaaca aggtcaaggt
    13321 ttaaagagag aggtgggttt gttatgagat ttaaaaatac atcggatcat attgaggcct
    13381 acatcaaggc gattttagat caatctggta tcgtggagtt gcaacggagt cagttggcag
    13441 atacctttca ggttgttcct agtcagatta actacgtgat caagacacgc tttacggaaa
    13501 gtagaggcta cttggttgaa agtaagcgtg gtggcggagg ctacattcgt ataggacgga
    13561 ttgagttttc tagtcatcat gaaatgctcc gggagctgct ttactcgatt ggtgagcgag
    13621 tcagtcaaga aatttatgag gatattctcc agcttttggt tgagcaggaa ttgatgacca
    13681 agcaggagat gaatttgcta gaatcagtag ctttggatcg cgttttagga gaagaagctc
    13741 cagttgttcg agcaaacatg ctacgtcagg tcatacaaga ggtagataga aaagggaagt
    13801 aagatgaact attcaaaagc attgaatgaa tgtatcgaaa gtgcctacat ggttgctgga
    13861 cattttggag ctcgttatct agagtcgtgg cacttgttga ttgccatgtc taatcacagt
    13921 tatagtgtag caggggcaac tttaaatgat tatccgtatg agatggaccg tttagaagag
    13981 gtggctttgg aactgactga aacggactat agccaggatg aaacctttat ggaattgccg
    14041 ttctcccgtc gtttgcaggt tctttttgat gaagcagagt atgtagcgtc agtggtccat
    14101 gctaaggtac tagggacaga gcacgtcctc tatgcgattt tgcatgatgg caatgccttg
    14161 gcgactcgta tcttggagag ggctggtttt tcttatgaag acaagaaaga tcaggtcaag
    14221 attgctgctc ttcgtcgaaa tttagaagaa cgggcaggct ggactcgtga agatctcaag
    14281 gctttacgcc aacgccatcg tactgtggct gacaaacaaa attctatggc caatatgatg
    14341 ggcatgccgc agactcctag tggtggtctc gaggactata cgcatgattt gacagagcaa
    14401 gcgcgttctg gcaagttaga accagtcatc ggtcgggaca aggaaatctc acgtatgatt
    14461 caaatcttga gccgtaagac taagaacaac cctgtcttgg ttggggatgc tggtgtcggg
    14521 aaaacagctc tggtgcttgg tcttgctcag cgtattgcca gtggtgatgt gcctgcggaa
    14581 atggctaaga tgcgcgtgtt agaacttgat ttgatgaatg tcgttgcagg gacacgcttc
    14641 cgtggtgact ttgaagaacg catgaataat atcatcaagg atattgaaga agatggccaa
    14701 gtcatcctct ttatcgatga actccacacc atcatgggtt ctggtagcgg gattgattcg
    14761 actctggatg cggccaatat cttgaaacca gccttggcgc gtggagcttt gagaacggtt
    14821 ggtgccacca ctcaggaaga atatcaaaaa catatcgaaa aagatgcggc tctttctcgt
    14881 cgtttcgcta aagtgacgat tgaagaacca agtgtggcag atagtatgac tattttacaa
    14941 ggtttgaagg cgacttatga gaaacatcac cgtgtacaaa tcacagatga agcggttgaa
    15001 acagcggtta agatggctca tcgttattta accagtcgtc acttgccaga ctctgctatc
    15061 gacctcttgg acgaagcagc agcaacagtg caaaataagg taaagtatgt aaaagcagac
    15121 gattcagatt tgagtccagc tgataaggcc ctgatggatg gcaagtggaa acaggcagcc
    15181 cagctaatcg caaaagaaga ggaagtacct gtctacaaag acttggtgac agagtctgat
    15241 attttgacca ccttgagtcg cttgtcagga atcccagttc aaaaactgac tcaaacggat
    15301 gctaagaagt atctaaatct tgaagcagaa ctccataaac gtgtcatcgg tcaagatcaa
    15361 gctgtttcaa gcattagtcg tgcgattcgc cgtaatcagt caggaattcg cagtcacaag
    15421 cgtccgatcg gttcctttat gttcctagga cccacaggtg tcggtaagac cgagttggcc
    15481 aaggctttgg cagaagttct ctttgatgac gaatcagccc ttatccgctt tgatatgagt
    15541 gagtatatgg agaaattcgc agccagccgt ctaaatggag ctcctccagg ctatgtgggt
    15601 tacgaagaag gtggagagtt gacagagaag gttcgcaata aaccctattc cgttctcctc
    15661 tttgatgagg tagagaaggc ccacccagat atttttaatg ttctcttgca ggttctggat
    15721 gacggtgttt tgacagatag caagggacgc aaggtcgatt tttcaaatac cattatcatt
    15781 atgacatcga acctaggtgc gactgccctt cgtgatgata agactgttgg ttttggggct
    15841 aaggatattc gttttgacca ggaaaatatg gaaaaacgca tgtttgaaga actgaaaaaa
    15901 gcttatagac cggaattcat caaccgtatt gatgagaagg tggtcttcca tagcctatct
    15961 agtgaccata tgcaggaagt ggtgaagatt atggtcaagc ctttagtggc aagtttggct
    16021 gaaaaaggca ttgacttgaa attacaagct tcagctctga aattgttagc aaatcaagga
    16081 tatgacccag agatgggagc tcgcccactt cgcagaaccc tgcaaacaga agtggaggac
    16141 aagttggcag aacttcttct taagggagat ttagtggcag gcagcacact taagattggt
    16201 gtcaaagcag gccagttaaa atttgatatt gcataaaaga ataaaagtat cagcatctga
    16261 ccataagtca cagtggagtg aaactcaatg aaaatcaaag agcaaactag gcagctagcc
    16321 gcaggttgct caaaacactg gtttgaggtt gcagatagag ctgacgtggt ttgaagagat
    16381 tttcgaagag tatgaaacta aaacctatag cttctaaacg atccgtggtt ttcaccattc
    16441 aacacaaaat tcatatgttt attaccctcc gtcgtatttg tcttagagcg tgtgtagtag
    16501 aaaaagagca gtcttatctg aaatttttat tctttcaaaa gagacctgtt tcttttttgc
    16561 atgtcaaatc cgttctagct ggtatttgaa aaatcaaact aatattcaat gaaaatcaaa
    16621 gaacaaacta ggcagctagc cacaggttgc tcaaaacact gttttgaggt tgtagataga
    16681 gctgacgtag tttgaagaga ttttcgaaga gtataagctg caagatgaat gattttcttg
    16741 tattgacgtt gttgttgaca aaaagtagcg gataaatgaa atccattcca ttatcataga
    16801 tgataggctg gtaggaaatt ttcaaatagc atacaggaaa tagatgtatg gagttctggt
    16861 agtagaaagg gagagagatg aacattttag ttgcagatga cgaggaaatg attagagaag
    16921 gaattgcagc atttctgaca gaagagggtt atcatgtcat tatggctaag gatggacaag
    16981 aggtcttgga aaaatttcaa gatctcccta tccatctcat ggtattggat ttaatgatgc
    17041 ctaggaagag tggttttgaa gtgttaaaag aaatcaatca aaagcacgat attcctgtca
    17101 tcgtcttgag tgctctggga gatgaaacta ctcagtcaca ggtatttgat ctctatgctg
    17161 atgatcatgt gacaaaacct ttttctttgg tactgcttgt caagcgtatt aaggcgctta
    17221 tcagacgtta ctacgtcata gaggatattt ggcgatatca ggatgtaaca gtggatttta
    17281 cctcttacaa agcacattat aaaaatgaag aaattgatct caaaccaaag gaattactgg
    17341 tactaaagtg tttgattcag cataaaaatc aagttttaag tagagagcag atattggaag
    17401 aaatttcaaa agatgtagct gatttacctt gtgatagggt cgttgatgtc tatattcgta
    17461 ctcttcgcaa aaaattagct ttagattgta tcgtgactgt gaaaaatgtt gggtataaga
    17521 ttagcttatg ataaaaaatc ctaaattatt aaccaagtct tttttaagaa gttttgcaat
    17581 tctaggtggt gttggtctag tcattcatat agctatttat ttgacctttc ctttttatta
    17641 tattcaactg gagggggaaa agtttaatga gagcgcaaga gtgtttacgg agtatttaaa
    17701 gactaagaca tctgatgaaa ttccaagctt actccagtct tattcaaagt ccttgaccat
    17761 atctgctcac cttaaaagag atattgtaga taagcgactc cctcttgtgc atgacttgga
    17821 tattaaagat ggaaagctat caaattatat cgtgatgtta gatatgtctg ttagtacagc
    17881 agatggtaaa caggtaacag tgcaatttgt tcacggggtg gatgtctaca aagaagcaaa
    17941 gaatattttg tttttgtatc ttccatatac atttttggtt acaattgctt tttcctttgt
    18001 tttttcttat ttttatacta aacgcttgct caatcctctt ttttacattt cagaagtgac
    18061 tagtaaaatg caagatttgg atgacaatat tcgttttgat gaaagtagga aagatgaagt
    18121 tggtgaagtt ggagaagttg gaaaacagat taatggtatg tatgagcact tgttgaaggt
    18181 tattcatgag ttggaaagtc gtaatgagca aattgtaaaa ttgcaaaatc aaaaggtttc
    18241 ctttgtccgt ggagcatcac atgagttgaa aaccccttta gccagtctta gaattatcct
    18301 agagaatatg cagcataata ttggagatta caaagatcat ccaaaatata ttgcaaagag
    18361 tataaataag attgaccaga tgagccactt attggaagaa gtactggagt cttctaaatt
    18421 ccaagagtgg acagagtgtc gtgagacctt gactgttaag ccagttttag tagatatttt
    18481 atcacgttat caagaattag ctcattcaat aggtgttaca attgaaaatc aattgacaga
    18541 tgctaccagg gtcgtcatga gtcttagggc attggataag gttttgacaa acctgattag
    18601 taatgcaatt aaatattcag ataaaaatgg gcgcgtaatc atatccgagc aagatggcta
    18661 tctctctatc aaaaatacat gtgcgcctct aagtgaccaa gaactagaac atttatttga
    18721 tatattctat cattctcaaa tcgtgacaga taaggatgaa agttccggtt tgggtcttta
    18781 cattgtgaat aatattttag aaagctatca aatggactat agttttctcc cttatgaaca
    18841 cggtatggaa tttaagatta gcttgtagac agattagttt tttattaaag ttcatatagg
    18901 gttaacataa gtgtgttatt ctttgtgtag ataaaagaaa ggatactaat atggtattag
    18961 cgattatttt agtaacattc tttattcgat tgattttttt aaagcgttcg atagagaatg
    19021 agaaacgaat ccttagcaat ggcgggaaag aatttggagt tgagaataca aaacgattaa
    19081 ctatggctca tattgttttt tatctctctt gcttggttga ggcaatggtg cacaagacaa
    19141 tttttgatgg catgggcatg gttggtttag tcttgcttat tttttctatg ctgatgttaa
    19201 tgttggtgat tcacttgttg ggagatattt ggacagtgaa gcttatgctt gtcaataatc
    19261 acaaatatgt agatcatatc ttgtttagga cagtaaaaca tcctaattac tttttaaata
    19321 ttcttcctga gttgattggc ttgaccttgt tgagtcatgc ttatgtgact tttgttttag
    19381 tttttccagt ttatgcagtt attttgtatc gacgaatagc tgaagaggaa aagctattac
    19441 atgaagttat aatcccaaat ggaagcataa agagataaat acaaaattcg atttatatac
    19501 agttcatatt gaagtgatat agtaaggtta aagaaaaaat atagaaggaa ataaacatgt
    19561 ttgcatcaaa aagcgaaaga aaagtacatt attcaattcg taaatttagt attggagtag
    19621 ctagtgtagt agttgctagc ttgttcttag gaggagtagt tcatgcagaa ggggttagaa
    19681 gtggggatac ccccaaggtt acatctagtg gggatgaagt cgagtcgcat ctaaaaaaaa
    19741 tattgagtga gatccaaaca caattagata gaaaacgaca tgccaaaact gtagcgctaa
    19801 ttaacaagtt gcaagacatt aagagaacgt attcgtataa attaaatgtt ttagaagaga
    19861 agccgaaagc tgagttgacg tcaaaaacaa aagaagagtt aaccgcagct tttgagcaat
    19921 ttaaaaaaga tacattacca acagaaccag gaaaaaaggt agcagaagct gagaagaagg
    19981 ttgcagaagc tgagaaaaaa gccgaggatc aaaaagaaga agatcgtcgt aactacccaa
    20041 ccaatactta caaaacgctt gaacttgaaa ttgctgagtt cgatgtgaaa gttaaagaag
    20101 cggagcttga actattaaaa gagaaagcta agaaaccttt aaacgagggc acaattaagc
    20161 aagcaaaaga gaaagttgag agtaaaaaag ctgaggctac aaggttagaa aacatcaaga
    20221 cagatcgtaa aaaagcagaa gaagaagcta aacgaaaagc agcagaagaa gataaagtta
    20281 aagaaaaacc agctgaacaa ccacaaccag cgccggctcc tcaaccagaa aaaccagctc
    20341 caaaaccaga aaaaccagct ccagctccaa aaccagagaa tccagctcca aaaccagaaa
    20401 aaccagctcc agctccaaaa ccagagaatc cagctgaaca accaaaagca gaaaaaccag
    20461 ctgatcaaca agctgaagaa gactatgctc gtagatcaga agaagaatat aatcgcttga
    20521 ctcaacagca accgccaaaa actgaaaaac cagcacaacc atctactcca aaaacaggct
    20581 ggaaacaaga aaacggtatg tggtacttct acaatactga tggttcaatg gcgacaggat
    20641 ggctccaaaa caatggctca tggtactacc tcaacagcaa tggcgctatg gcgacaggat
    20701 ggctccaaaa caatggttca tggtactatc taaacgctaa tggttcaatg gcaacaggat
    20761 ggctccaaaa caatggctca tggtactacc taaacgctaa tggttcaatg gcgacaggat
    20821 ggctccaata caatggttca tggtactacc taaacgctaa tggtgatatg gcgacaggat
    20881 ggctccaata caatggttca tggtactacc taaacgctaa tggtgatatg gcgacag
//
LOCUS       DAVBCR010000031        20630 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCR010000031 DAVBCR010000000
VERSION     DAVBCR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.031"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VQ508_001097"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VQ508_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2490103.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VQ508_001098"
     CDS             2483..4144
                     /locus_tag="VQ508_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2490104.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VQ508_001099"
     CDS             4213..4992
                     /locus_tag="VQ508_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2490105.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VQ508_001100"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VQ508_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2490106.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VQ508_001101"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VQ508_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2490107.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VQ508_001102"
     CDS             7640..8341
                     /locus_tag="VQ508_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2490108.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ508_001103"
     CDS             8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ508_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2490109.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10218..11051
                     /locus_tag="VQ508_001104"
     CDS             10218..11051
                     /locus_tag="VQ508_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2490110.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11233..12033)
                     /locus_tag="VQ508_001105"
     CDS             complement(11233..12033)
                     /locus_tag="VQ508_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2490111.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12204..12809
                     /locus_tag="VQ508_001106"
     CDS             12204..12809
                     /locus_tag="VQ508_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2490112.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12822..14222
                     /gene="pepV"
                     /locus_tag="VQ508_001107"
     CDS             12822..14222
                     /gene="pepV"
                     /locus_tag="VQ508_001107"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2490113.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14276..14854
                     /locus_tag="VQ508_001108"
     CDS             14276..14854
                     /locus_tag="VQ508_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2490114.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15014..15166
                     /locus_tag="VQ508_001109"
     CDS             15014..15166
                     /locus_tag="VQ508_001109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2490115.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15209..15301
                     /locus_tag="VQ508_001110"
     CDS             15209..15301
                     /locus_tag="VQ508_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2490116.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15472..16797
                     /gene="brnQ"
                     /locus_tag="VQ508_001111"
     CDS             15472..16797
                     /gene="brnQ"
                     /locus_tag="VQ508_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2490117.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIANVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16895..17932
                     /locus_tag="VQ508_001112"
     CDS             16895..17932
                     /locus_tag="VQ508_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2490118.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            17934..18404
                     /locus_tag="VQ508_001113"
     CDS             17934..18404
                     /locus_tag="VQ508_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2490119.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            18407..19123
                     /locus_tag="VQ508_001114"
     CDS             18407..19123
                     /locus_tag="VQ508_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2490120.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            19246..19671
                     /gene="rplK"
                     /locus_tag="VQ508_001115"
     CDS             19246..19671
                     /gene="rplK"
                     /locus_tag="VQ508_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2490121.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            19878..20567
                     /gene="rplA"
                     /locus_tag="VQ508_001116"
     CDS             19878..20567
                     /gene="rplA"
                     /locus_tag="VQ508_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2490122.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6206 a         3799 c         4776 g         5849 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag gaatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaaga cctattatct
     1981 gacaggttat gggactagag atcagtttgc taaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacaca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag atcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcatacgcgc ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggttacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagcgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttga atttttcgga gtatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatg ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aattcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttatcgt
     8581 cactgagtcc aatattgagg cgcttctgct ggaaatcaac ttgatcaagg aaaataagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga
     8701 gcgctatcca cggcttatca ttactcgtca ggtcaaaaag gacggtggtc tttattttgg
     8761 cccttatcca gatgtggggg cagccaatga aatcaagcga cttctagata ggattttccc
     8821 ttttcgaaaa tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggccagtg
     8881 tatggcccac accatctgta agaaggatga ggcttatttc aagtctatgg ctcaggaggt
     8941 gtctgatttt ctgaaaggtc aggatgacaa aatcatcgat gatctcaaga gtaaaatggc
     9001 agtagcagca caaagtatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg ttcgtcaggg
     9181 caagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 cttgacctat gtgggacaat tctatcaaga aaaatctcac ctagttccca atgaggtact
     9301 gattccgcag gatattgacg aagaagctgt caaggctttg gtggatacca agattctcaa
     9361 accccaacgt ggagagaaaa aacagctggt caatctagcc ataaaaaatg cacgtgttag
     9421 tctggagcag aaatttaacc tgctagaaaa atcagtcgaa aagacccaag gagctattga
     9481 aaatctagga cgtttgctcc aaattccgac tccagttcgc atcgagtcct tcgataactc
     9541 caacatcatg gggaccagtc ctgtttcagc catggtggtc tttgttaacg gcaggccaag
     9601 taaaaaagat taccgtaagt acaagataaa aaccgttgtt gggccggacg actatgctag
     9661 tatgagagaa gtcattcgaa gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat attgctaagc aagtcatcca
     9781 agaagaactg ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac
     9841 ccatgaattg ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca ttcaagatga ggtccaccgc tttgccatta ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaagaaa
    10141 gttgaacccg caggaaacag aaatcttgct tcaagtggcg gaagaaagag tagattacca
    10201 aacggaagga aaccacaata aaccataaaa tcgcaatttt atcagatatt catggcaatg
    10261 cgacggcgct agaagcagtg attgcagatg ctaaaactca aggagccagt gaatattggc
    10321 tcatgggaga cattttcctc cctggttcag gtgcaaatga cttggtcgct ctgttaaagg
    10381 aacttcctat cacggcaagt gttcgaggca attggaatga ttgtgtcctt gaggctttag
    10441 atgggcaata tggcttagaa gacccacagg aaatccagct catgcgaatg actcagtttt
    10501 tgatggagcg aatggatcct gcaacgactg tctggctacg aagcttgcct ttgctggaaa
    10561 agaaagaaat tgctggactg cgcttttcta tctctcataa tttgccaaat aagaactatg
    10621 gtggtgactt gctagttgag aatgatacag agaaatttga ccaactgcta gatgcggaaa
    10681 cggacgtggc agtttatggt catgttcaca agcagttgct tcgttatgga agtcaagggc
    10741 aacaaatcat caatccaggg tcgattggca tgccctattt taattgggag gcgttaaaaa
    10801 atcaccgtgc ccagtatgcc gtgatagaag ttgaagatgg ggaattggtc aatatccaat
    10861 ttcgtaaagt tgcttatgat tacgaggctg agttagaatt ggccaagtcc aaggggcttc
    10921 cctttattga aatgtatgaa gaactgcgtc gtgacgataa ctatcagggg cacaatctgg
    10981 aacttttagc cagcttaata gaaaagcatg ggtatgtaga ggatgtgaag aatttttttg
    11041 attttttgta agagtttcct aaaatagcca atgcaaacta aaaaagcgat tggctggtcc
    11101 aatcgctttt agtatatctt atactcaatg aaaatcaaag agcaaactag gaagctagcc
    11161 gtaggttgct caaagcacag ctttgaggtt gcagataaag ctgacgtggt ttgaagagat
    11221 tttcgaagag tgttattgta actgagattg atctgggagg taagaaccac ctagataggt
    11281 attgctgagt ttttcaaggg ttccgtcttg atagagttct ttgagtgctt tatcaaattg
    11341 ctctttaaac tctttttggt cgcttgagaa aatgatataa ttgctggggc tatctgcaga
    11401 aggtaaatca acgactgaga ggtctaaacc acggtccttg ataatctttt gaacggatac
    11461 cttgtcaaaa actaggaaat caaactctcc gttagcaagg tctaggattc gtttaccaat
    11521 atcctcacca gaaaaattaa ttgtagcggg attatcagtg tgtttctgat tccagttatt
    11581 gatgaattga gcgttagaag ttccggtatc ctcttgtgtt gttttaccag cgatctggtc
    11641 aagagaagtc aaaggatttt tcttgttgct gacaaggacg aggggattgt tggaaatcgg
    11701 aagcgagtaa aggtattttt cagcacgctc ttttgtgtaa ctcaaattat tggccgcagc
    11761 ctgatagtga ccagaatcaa gtcctgggaa gatactttcc caggcggttc tttgaaattg
    11821 aatctcgtag tcgctgagtt tttcatctac tgcctttaga acttcgatat cgaagcctgt
    11881 cagattgcct ttatcttcgt agtcaaatgg tggcacgtcg ccagctgtag caaggacgat
    11941 tgtcttttga gcgctagtct ctttgggtgt agcttgattc ccacaggcaa ccaaaaatgg
    12001 taggatagct agtaataggc taaatttttt catactgtct ccattcaaat gtaaagtact
    12061 tgctagttta tcagaaactt tcctgataaa ccaatatata tcttttatgt ctgtgataga
    12121 aaaacttaat catgaaaaat ccctgcaagc tctttcaaat tatggtagaa tggaagcagt
    12181 agaattagaa aaggaaatcg attatgaaat ttcttgaatt aaataaaaaa cgtcatgcga
    12241 ccaagcattt cactgataag cctgttgatc taaaagatgt gcgtacggct atcgaaattg
    12301 caaccttggc gccaagcgcc cacaacagcc agccttggaa atttgtagtt gttcgtgaga
    12361 aaaatgctga actggcaaag ttggcttatg gttcaaactt tgagcaggta tcatcagcac
    12421 ctgtaaccat tgccctattt acagatacag accttgccaa acgcgctcgt aagattgccc
    12481 gagttggtgg tgctaacaat ttctcagaag aacaacttca atacttcatg aaaaatttgc
    12541 cagctgaatt tgcacgctac aatgaacaac aagtcagcga ctacctagca ctcaatgcag
    12601 gtttggttgc tatgaacttg gttctcgctc ttacagacca aggaattggt tccaatatta
    12661 tccttggttt tgacaaatca aaagtcaatg aggttttgga aattgaagac cgtttccgcc
    12721 cagaactctt gatcacagtg ggttacacag acgacaaatt ggaaccaagc taccgcttgc
    12781 cagtagatga aatcatcgaa aaaagatagg aagaagaaaa aatgatagca attgatttta
    12841 cagcagaagt agaaaaacgc aaagaagacc tcttggctga cttgtttagc cttttggaaa
    12901 tcaactcaga acgtgatgat agcaaggctg atgcccaaca tccatttgga cctggtccag
    12961 taaaagcctt ggaaaaattc cttgaaatcg cagaccgtga tggttatcca actaaaaatg
    13021 ttgataacta tgcaggacat tttgagtttg gtgatggaga agaagttctc ggaatctttg
    13081 cccatatgga cgtggttcca gctggtagcg gttgggacac agacccttac acaccaacta
    13141 tcaaagatgg tcgcctttat gcgcgtggtg cttcagatga caagggacct acaacagctt
    13201 gctactatgg tttgaaaatc atcaaagaat tgggccttcc aacttctaag aaagttcgct
    13261 tcatcgttgg aacagacgaa gaatcaggct gggcagatat ggactactac ttcgagcacg
    13321 taggactcgc caagccagac tttggtttct caccagatgc tgaatttcca atcatcaatg
    13381 gtgaaaaagg aaacatcacg gaatacctcc actttgcagg tgaaaataca ggtgcagccc
    13441 gtcttcacag ctttacaggt ggtttacgtg aaaatatggt accagaatca gcaacagcag
    13501 tcgtttcagg tgacttgaca gacttgcaag ctaaactaga tgcctttgtt gcagaacaca
    13561 aacttagagg agaactccaa gaagaagctg gccaatacaa ggtgacgatc attggtaaat
    13621 cagcccacgg tgctatgcct gcttcaggtg tcaatggcgc aacttacctt gccctcttcc
    13681 tcagccagtt tggctttgct ggtccagcca aagactacct tgacatcgca ggtaaaattc
    13741 tcttgaacga tcatgagggt gaaaatctca agattgctca tgtggatgaa aagatgggtg
    13801 ctctttctat gaatgccggc gtcttccgct tcgatgaaac aagtgctgat aataccattg
    13861 cccttaacat ccgctatcca aaaggaacaa gtccagaaca aatcaagtca atcctagaaa
    13921 acttgccagt tgcttctgtc agtctatcag aacatggtca tacccctcac tatgtgccaa
    13981 tggaagatcc acttgtgcaa accttgttga atgtctatga aaaacaaaca ggccttaaag
    14041 gtcatgaaca agtcatcggt ggtggaacct ttggtcgctt gttagagcgc ggagttgcct
    14101 atggtgctat gttcccagac tcaattgata ccatgcacca agccaatgaa tttattgcct
    14161 tggacgatct cttccgagca gcagcaattt atgccgaagc tatttacgaa ttgatcaaat
    14221 aaaacgatag aagtctgaga tcttatgctt ggacttcttt ttggagggaa agtagatgtc
    14281 tcaaatcgaa agaatcaaac aggctatcat ggcggattcg cagaatgcca gctatacaga
    14341 gcgtggcatt gagcctctct ttgcagcgcc aaaaactgct cgcatcaata tcatcggtca
    14401 ggctccggga cttaaaactc aagaagcagg cctttactgg aaagataaaa gtggtgaccg
    14461 tttgcgggac tggctaggtg tggatgaaga taccttttac aattcaggtt attttgctgt
    14521 tttgcctatg gatttctact ttccaggaca tggcaagtcg ggtgatcttc cgccttgtac
    14581 aggttttgca gaaaaatggc atccgcagct cttacaggaa ttgcctgata ttcagttaac
    14641 cctcttgatt gggcaatatg cccaagccta ctatttacag gagaaaatca gtgggaaggt
    14701 aacggagagg gtgaaacact ataaagacta tctgccagcc tattttccgc tagttcaccc
    14761 atcaccacga aatcaaatct ggatggccaa aaatccttgg tttgaggcag aagtagtgcc
    14821 agatttgaaa aaaagaatta aaaccatttt atagtcaatg aaaatcaaag agcaaactag
    14881 gaagctagtc gtaggctgct caaagtacag ctttgaagtt gcagataaaa ctgacgaagt
    14941 cggtaacata cgcacggtaa ggcgacgctg acgtggtttg aagagatttt cgaagagtat
    15001 tagaagaaaa agaatgaaag aaatagcctt tgacgcattt taccagcttt accaaaacga
    15061 ccagctttct ctagtggatg tgagagaagt ggatgagttt gcagctcttc atttagaagg
    15121 tgcccacaac ctaccgctta gtcaattggc tgatagttat gattaattgg acaaagatcg
    15181 cttgcattat attatttgca aatctggaat gagatcggcg cgtgcttgcc aattcctatt
    15241 agaacaaggt tataatgtta tcaatgtcca gggtggcatg ttagcctttg aagaacttta
    15301 aaattttgca tttctcctac ttggtgtgga ctgggtagga gagttttatt tttagataat
    15361 tcttattttt aagaaaattg aaaacattta atatttgcct cgtgatgctt ttttcagact
    15421 cctaatcgtg gtatactaga tcagtatttt ataaatatga aggagatttt tatggctaaa
    15481 aaaggtaccc taacaggttt gctcctgttt ggaatatttt ttggtgcggg gaacttgatt
    15541 tttccgcctt ctctaggtgc tctatctgga gaacattttc ttcctgccat cgcaggtttt
    15601 gtcttttcag gcgttggtat cgccgtcttg acccttatta ttggaacgct aaatcctaaa
    15661 ggatatatct acgagatttc aacgaagata gcgccttggt ttgcgactct ttacctctca
    15721 gttctttact tgtcaatcgg tccattcttt gctaccccac gtactgctac aacagcttac
    15781 gaagtaggga ttagccccct tttgtcggat gcaaataaag gacttggctt gattgtattt
    15841 acggttctgt attttgcggc agcctatttg atttcgctta atccatcaaa aatcttagac
    15901 cgcattggac gtattttaac gccagtcttt gcaattttga ttgttatctt ggtcgttctg
    15961 ggagctatca aatatggtgg aacaagtcct caagctgctt cagctgctta tcaagcttct
    16021 gcctttggta caggtttcct agaaggttat aataccttgg acgcccttgc ctcagtggcc
    16081 tttagcgtaa tcgcagttca aaccttgaaa caacttggat tttcaagtaa gaaagaatac
    16141 atttcaacta tttgggttgt tggtatcgtt gttgcccttg ccttcagcgc tctttacatc
    16201 ggtttaggtt ttcttggaaa tcatttccca gtaccagctg aagcgatgaa gggtggaaca
    16261 ccaggtgttt acatcttgtc acaagccact caagaaatct ttggctcaac agctcaactc
    16321 ttccttgcag ctatggttac cgtaacctgc ttcacaacga ctgttggttt gattgtgtca
    16381 acagctgagt tctttaatga gcgcttccca caaatcagct acaaggttta tgcgacagcc
    16441 tttaccttga ttggatttgc tattgccaat ttgggtcttg atgcgattat caagtactca
    16501 attccagtac tggttatctt gtacccaatc acgattgcta tcgttatgat tgtcattgtc
    16561 aacaaatttg ttgccctttc aaaaccaggt atgcagttga caattgctgt ggttacagtt
    16621 attgccattg caaacgtact aggaagctcg tttaaggttg agtttcttgc aaatcttgtt
    16681 agcgttcttc cttttgccaa ggcatctctc ccatggttgg tgccagccat tgttggaatc
    16741 ttgctctcat tggttctacc aaacaagcaa gaaagcgatg tttttgaaat ggaataatca
    16801 cttaaatcac ttttgtagcc aagtctacag gagtgatttt ctttttttat ccgatgataa
    16861 atgtgttata ataggtagcg aaagaggtga agaaatgaat caaacagtag aatatatcaa
    16921 agaactgaca gccatcgcat cgccaacggg cttcactcgt gaaatttcga attatttagt
    16981 caagactcta gaaggttttg gttaccagcc agttcgcaca gccaagggtg gtgtcaatgt
    17041 gaccatcaaa ggtcaaaatg atgagcaaca tcgctatgtg actgcccatg tggacacact
    17101 gggtgctatt gttcgtgctg tcaaaccaga tggtcgtctt aaattggacc gtatcggtgg
    17161 ttttccttgg aacatgattg aaggtgaaaa ctgtaccgtt catgtggcta gcacaggtca
    17221 aaaggtatta ggaaccatcc tcatccacca aacttcttgc catgtctaca aggatgcagg
    17281 aactgcagaa cgcacgcagg acaatatgga agtgcgtttg gatgtaaaag taagcaatga
    17341 aaaagaaact cgtgctcttg ggattgaggt cggtgatttt atcagcttcg atccacgaac
    17401 tgttgtgact gatacaggtt ttatcaagtc tcgtcatttg gatgataagg tcagcgcagc
    17461 gatgttgctc aacctgcttc gtatttataa gaaagagaag attgaattgc cagtaacaac
    17521 tcattttgct ttttcagttt ttgaagaagt aggacatggt gctaattcta acattcctgc
    17581 tcaggtagta gaatatctgg ctgtggatat gggagctatg ggagatgacc agcagacaaa
    17641 tgagtacaca gtatctatct gtgtcaagga tgcctcaggt ccttatcact atgacttccg
    17701 tcaacacttg gttgccttgg ctaaagagca agatattcct tttaagctgg acatctatcc
    17761 attttatggt tcggatgctt cagcggctat gtctgcaggg gcagaggtca agcatgccct
    17821 tcttggtgcg ggtatcgagt ctagtcattc ttatgagcgc actcatattg actcggtggt
    17881 cgcaacagaa cgaatggtcg atgcctatct taagagtgca ttggtagact aatatgtgtt
    17941 tgatttgcca gagaattgag ctcatcaaga agggagaaaa tccctatttt gtcaaagagt
    18001 tggaaacagg ctatcttgtg attggagacc accagtattt tgcaggctat agtctctttc
    18061 tagccaagga acatgtcagc gaattgcatc atttagaaaa gaaaactaga atacgttttc
    18121 tagaagaaat gagtgtggtc caagaggcag ttgccaaggc ctttgctgct gagaaaatga
    18181 atatcgaact actaggaaat ggcgatgctc atctccattg gcatctgttt cctagacgaa
    18241 gaggtgatat gaatggttat ggcctcaatg gacgtggtcc agtatggtgg attccctttg
    18301 aagaaatgac agcagaaatc tgccaatcaa aacctgatga gattaaaaga ttagtcgaac
    18361 gtttatcatc agaagtagat aaactattag aaataaagga gtaaagatga aaaaaagata
    18421 ccttgtcttg acagccttgc tagccttgag tctagcagct tgttcacaag aaaaagcaaa
    18481 aaatgaagat ggagcagcta agacagagca aactgctaaa gctgatggaa cagtaggaag
    18541 taagtctcaa ggagctgccc agaaaaaagc agaagtggtt aacaaaggag actattacag
    18601 cattcaaggg aaatacgatg aaatcatcgt agccaacaaa cactatccat tgtcaaaaga
    18661 ctacaatcca ggggaaaatc caacagccaa ggctgagttg gtcaagctca tcaaagctat
    18721 gcaagaggca ggtttcccca ttagcgatca ttacagtggc tttagaagtt atgaaactca
    18781 gactaagctc tatcaagatt atgtcaatca agatgggaaa gaagctgccg accgttattc
    18841 tgcccgtcct ggctatagcg agcaccagac aggtttagcc tttgatgtga ttgggacaaa
    18901 tggtgatttg gtgacagaag aaaaagcggc ccaatggctc ttggatcatg cggctgatta
    18961 tggctttgtt gtacgttatc tcaaaggtaa ggaaaaggaa acaggctata tggctgagga
    19021 atggcacctt cgttatgtcg gaaaagaggc taaagagatt gcagctagtg gcctcagttt
    19081 ggaagagtat ttcggctttg aaggtggaga ttacgtcgat taaaaaataa acttttctct
    19141 tgcaattcca gataaatagt gtataataga tgggtatgtg aaaaacatac ttgtgggagg
    19201 taaaaatctc taattaccgc caaaaccaca aaggaggatt taaaaatggc taaaaaagtc
    19261 gaaaaacttg taaaattgca aatccctgct ggtaaagcta caccagctcc accggttgga
    19321 cctgctcttg gtcaagctgg tatcaacatc atgggattca caaaagagtt caacgctcgt
    19381 acagctgacc aagctggtat gatcattcca gttgttatct cagtttacga agataaatca
    19441 tttactttcg ttacgaaaac accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt
    19501 gaaaaaggat caggtacacc taataaaact aaagttgcta cagttactcg tgcacaagta
    19561 caagaaattg cagaaactaa gatgccagat ttgaacgcag caaacgtaga gtctgcagtg
    19621 cgtatgatcg aaggtactgc tcgttctatg ggattcactg ttattgacta atcaataaca
    19681 cagtagaaaa tctcacagca gtctattagt tgcttttcat actaggcaag tgactgatgc
    19741 ttgtacttgg gtacagcaag ggagcttaag gccgtaggaa aagaatctaa tagactgaaa
    19801 acccgcaaga cttcatcatt tcgagaagtg acgtgggaga tgaaaatcga ttgaaccact
    19861 tacaaggaga atagaaaatg gctaaaaaaa gcaaacaact tcgtgctgct cttgagaaaa
    19921 tcgacagcac aaaagcatac agcgtagaag aagctgtagc acttgcaaaa gaaacaaact
    19981 ttgcaaaatt tgacgcaact gtagaagttg cttacaactt gaacatcgac gttaaaaaag
    20041 ctgatcaaca aatccgtgga gcaatggtat tgccaaacgg tactggtaaa acttcacgcg
    20101 ttcttgtttt cgcacgtggt gcaaaagctg aagaagcaaa agctgctggt gcagactttg
    20161 ttggtgaaga tgaccttgtt gctaaaatca acgacggttg gttggacttc gacgtagtta
    20221 tcgctacacc tgatatgatg ggtcttgttg gacgtcttgg acgtgtcctt ggaccacgta
    20281 acttgatgcc aaaccctaaa actggtactg taacaatgga tgttgctaaa gcagttgaag
    20341 agtctaaagg tggtaaaatc acttaccgtg ctgaccgtgc aggtaacgtt caagcaatca
    20401 tcggtaaagt atcatttgaa gctgaaaaat tggttgaaaa ctttaaagct ttcaacgaaa
    20461 caatccaaaa agcaaaacca gctacagcta aaggaactta cgtaacaaac ttgactatca
    20521 caactactca aggtgttggt atcaaagttg acgtaaactc actttaatca gtagttaaag
    20581 taatgtaaaa aagttgaaga cgctatgtct caactttttt tgatgtacga
//
LOCUS       DAVBCR010000032        20510 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCR010000032 DAVBCR010000000
VERSION     DAVBCR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.032"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            210..623
                     /gene="rpsL"
                     /locus_tag="VQ508_001117"
     CDS             210..623
                     /gene="rpsL"
                     /locus_tag="VQ508_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2490123.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            643..1113
                     /gene="rpsG"
                     /locus_tag="VQ508_001118"
     CDS             643..1113
                     /gene="rpsG"
                     /locus_tag="VQ508_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2490124.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1538..3619
                     /gene="fusA"
                     /locus_tag="VQ508_001119"
     CDS             1538..3619
                     /gene="fusA"
                     /locus_tag="VQ508_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2490125.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3723..8114
                     /locus_tag="VQ508_001120"
     CDS             3723..8114
                     /locus_tag="VQ508_001120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2490126.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8216..8479
                     /locus_tag="VQ508_001121"
     CDS             8216..8479
                     /locus_tag="VQ508_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2490127.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8472..8750
                     /locus_tag="VQ508_001122"
     CDS             8472..8750
                     /locus_tag="VQ508_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2490128.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8771..8923
                     /locus_tag="VQ508_001123"
     CDS             8771..8923
                     /locus_tag="VQ508_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490129.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8945..10186
                     /locus_tag="VQ508_001124"
     CDS             8945..10186
                     /locus_tag="VQ508_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2490130.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENVGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10196..10426
                     /locus_tag="VQ508_001125"
     CDS             10196..10426
                     /locus_tag="VQ508_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2490131.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10637..11359
                     /locus_tag="VQ508_001126"
     CDS             10637..11359
                     /locus_tag="VQ508_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2490132.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVMDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11576..12910
                     /gene="pepC"
                     /locus_tag="VQ508_001127"
     CDS             11576..12910
                     /gene="pepC"
                     /locus_tag="VQ508_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2490133.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(12966..13877)
                     /locus_tag="VQ508_001128"
     CDS             complement(12966..13877)
                     /locus_tag="VQ508_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2490134.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13901..14704)
                     /locus_tag="VQ508_001129"
     CDS             complement(13901..14704)
                     /locus_tag="VQ508_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2490135.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14732..15721)
                     /gene="manX"
                     /locus_tag="VQ508_001130"
     CDS             complement(14732..15721)
                     /gene="manX"
                     /locus_tag="VQ508_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2490136.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(15954..16973)
                     /gene="adhP"
                     /locus_tag="VQ508_001131"
     CDS             complement(15954..16973)
                     /gene="adhP"
                     /locus_tag="VQ508_001131"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2490137.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKNLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17312..18124)
                     /locus_tag="VQ508_001132"
     CDS             complement(17312..18124)
                     /locus_tag="VQ508_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2490138.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18314..19732
                     /locus_tag="VQ508_001133"
     CDS             18314..19732
                     /locus_tag="VQ508_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2490139.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLTMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNDENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19785..20492
                     /locus_tag="VQ508_001134"
     CDS             19785..20492
                     /locus_tag="VQ508_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490140.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
BASE COUNT         5971 a         4024 c         4673 g         5842 t
ORIGIN      
        1 cagaaattga tctctaacat taccaataaa aatgaaaaat atcttgacaa gcaagggaaa
       61 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
      121 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
      181 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
      241 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
      301 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
      361 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
      421 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
      481 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
      541 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
      601 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
      661 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
      721 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
      781 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
      841 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
      901 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
      961 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
     1021 gctgctaaca acactggtgc agctgttaag aaacgtgaag atactcaccg tatggctgaa
     1081 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaagt
     1141 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
     1201 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
     1261 gtgaaaatag gaaactgacg cagtattcaa cgaatacaag gagttttatc tttttcacgc
     1321 agcatcccgt tccagctcac atcggctaac taactttagc ccgggttcaa attagctaaa
     1381 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
     1441 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
     1501 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
     1561 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
     1621 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
     1681 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
     1741 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
     1801 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
     1861 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaactg agtacggagt
     1921 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
     1981 aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
     2041 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
     2101 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
     2161 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
     2221 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
     2281 tatcaacgtt gaattcttcc cagtattatg tggttcagcc ttcaaaaaca aaggtgttca
     2341 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
     2401 aggtattaac ccagatacag acgctgaaga aactcgtcca gcatctgacg aagagccatt
     2461 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
     2521 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
     2581 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
     2641 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
     2701 gacagatgaa aaatctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
     2761 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
     2821 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
     2881 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
     2941 caaagttgaa gcgaacgtag gtgcgcctca agtatcttac cgtgaaacat tccgcgcttc
     3001 tactcaagca cgtggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
     3061 gtggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
     3121 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
     3181 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
     3241 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
     3301 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
     3361 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     3421 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
     3481 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
     3541 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
     3601 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
     3661 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
     3721 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
     3781 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
     3841 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
     3901 agaaaggttt gagcgaagaa ttttctaaga caggcaataa agctgttttt gaaattaagg
     3961 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
     4021 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
     4081 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
     4141 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttcaaact tttaactgtc
     4201 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
     4261 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
     4321 tggcacctcc tccagtggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
     4381 ctaaacccaa actggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
     4441 atcgtctggt atttgaaggg gttgtttttg atgtggagca aaaagtgact agaacaggtc
     4501 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
     4561 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
     4621 ttcgagggaa tgtggagatg aataacttca cacgcgattt gactatgaac gtacaggatc
     4681 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
     4741 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
     4801 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
     4861 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
     4921 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
     4981 tatcagaagc aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
     5041 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
     5101 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
     5161 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
     5221 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
     5281 ctaattatga gcggcatgac cttccaaaga ttagtcagcc agttattgat acgctggagt
     5341 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
     5401 ttggtgtggc cttggaacat caccacatgg ccaactacga tgcggaagcg actggtcgtc
     5461 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
     5521 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
     5581 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
     5641 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
     5701 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
     5761 gtgtggatgc ggcggttgag gtggccaagt attatgattt tatcgaggtc atgccaccgg
     5821 ctatctatgc acccttgatt gccaaagagc aggtcaagga tatggaggaa ctccagacca
     5881 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttctg gctacgggaa
     5941 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
     6001 gacagggtgc gatgattaat cgaactatcg gtcatggtga acatgcccaa ccagcaccac
     6061 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
     6121 aggaactggc tcgtaaactg gttattgaaa acaccaatgc cttggcagaa atatttgaac
     6181 ccgttgaagt cgttaagggt gacttgtata cgcctttcat cgacaaggct gaagaaacag
     6241 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
     6301 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
     6361 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
     6421 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg gattacggag gtcaatcctc
     6481 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
     6541 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
     6601 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
     6661 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
     6721 tctttggtga agaatatgcc ttccgtgcgg gaacggttgg tacggtagct gccaagactg
     6781 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagagg
     6841 tggaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
     6901 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
     6961 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
     7021 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
     7081 atttgtctgg tattgaccct aatgaaattc ctatggatga cgaaggcgtg atggcactct
     7141 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
     7201 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
     7261 caacctttgc ggaattgctt cagctgtctg gtctgtcaca cggtactgac gtttggttgg
     7321 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
     7381 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
     7441 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatgact
     7501 atatcgaagc catgaaggct aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
     7561 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
     7621 cttacttcaa ggttcaccat cctatttatt actactgtgc ttacttctcc attcgtgcta
     7681 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
     7741 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
     7801 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggtaaa ttagatctct
     7861 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
     7921 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
     7981 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
     8041 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
     8101 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
     8161 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaatagggtg agagtatgtc
     8221 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
     8281 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
     8341 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagtgtt
     8401 aacagattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
     8461 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
     8521 ctatgaagcg tggtttgaag gccgaattat tagaagaagt tttgaatttt ctggttcaag
     8581 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
     8641 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
     8701 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
     8761 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
     8821 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
     8881 tattgcttta gcaggaatct tttctgcctt gaaagaaaaa taaaaataga aaagagaaaa
     8941 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
     9001 gaacggaatt aacgtgcaac ctggtcacac tttggctctc tctattgatg tggagcaacg
     9061 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
     9121 tcagtggaca gatgatgtca ttaaccgtga gaaattcctc catgccccga tggagcgttt
     9181 ggacaatgtg ccagaataca agattgctga gatgaactat cttttggaga acaaggctag
     9241 ccgtcttggg gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
     9301 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
     9361 taacaaggtt agctggactg tagcagctgc agcaggactt gagtgggcta agaaagtctt
     9421 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggaccaaa ttttcaaaac
     9481 ttgccgtgtc tatgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
     9541 gagcaaggca gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
     9601 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
     9661 tgcacagggc gaaggattct tgccaaatat gccgacagaa gaggtcttca cagcgcctga
     9721 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
     9781 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
     9841 gggtgatcag gttatgaaag accttgtctt tgaaaatgtg ggtgcgcgtg ccttgggtga
     9901 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tctttaacac
     9961 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    10021 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    10081 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    10141 tggaacgcga gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    10201 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    10261 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    10321 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    10381 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatctga
    10441 tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcactgc
    10501 tttgaggttg cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga
    10561 cgcggtttga agagtatgaa acgaaaagga ggttggtcat tgtaccagcc tccttttgat
    10621 atgatataat agttctatga gattagataa atttttagtt gcctgtgcgg tgggaagtcg
    10681 gactgaggtc aaaaacttgc tcaaggctgg gcgcgtgact gtaaatggta aaaaagaaaa
    10741 atcagctaaa ttgcagattg atgaaaaaat agatgagatt cgctttgatg ggcaagtgtt
    10801 ggagtatgaa gagtttgtct actacatgat gaacaagccc aaaggagtta tctcagcgac
    10861 tgaggatccc aagcacagaa ccgttatgga cttgttggat gacttggcgc ggagtaagga
    10921 agttttccca gtaggacgct tggatattga cacgcatggt cttttgctct tgaccaatga
    10981 tggtcagctg gctcatgttc ttctttcgcc caagcgtcat gtggacaaga cttatctggc
    11041 acaagtcaag ggaatcatga cccaagaaga tgtggagaca tttgctgagg gtattcctct
    11101 caaagacttt acctgtcaac ccgctatact ggagcttgta tccatagata cagaaaagaa
    11161 tcaaagccaa atccgtgtga ccattgcaga agggaagttt catcagatca agcgtatggt
    11221 gggctactgt ggcaaggaag tggtggactt gcaacgattg actatgggaa ctttagtatt
    11281 agatgagaac ctggaacgag gggaatggcg tcgcttgacc aaggaagaat tagaaattct
    11341 ccgcgctaat attatttaat ttagtttaat actcaatgaa aatcaaagag caaactagga
    11401 aactagccgc aggctgtact tgagtacggc aaggcgacgt tgacgtggtt tgaagagatt
    11461 ttcgaagagt ataaattcga aaaaggtgag ggacaatgtc ctcgcctttt atgtttttta
    11521 gttgcttcct ttgtgaaaag agttataata gactgtagaa taaaaggagg aatctatgaa
    11581 cgcgattcaa gaatcattta ctgataaact atttgccaac tatgaagcaa atgtaaaata
    11641 ccaagcgatt gaaaatgctg ccagccacaa cggaattttt gcagctctgg aacgtcgcca
    11701 aagccatgta gacaacacac ctgttttctc attggattta accaaggaca aggtcactaa
    11761 ccagaaagcg tctggtcgtt gctggatgtt tgcggctctc aacaccttcc gccacaaact
    11821 catctcgcaa tacaaattgg agaactttga gttgtcacaa gcccacactt tcttctggga
    11881 taagtatgag aaatcaaact ggttcttgga gcaagtcatt gcgacttcag accaagaatt
    11941 gactagccgc aaggttagct tcttactcca aacacctcaa caagatggtg gtcaatggga
    12001 tatggtcgtt tccctctttg aaaaatacgg tgtcgtgcct aagtcagttt atcctgagtc
    12061 tgtttcatct agcagcagtc gtgagctaaa tgcgatcctt aataaattgc ttcgtcaaga
    12121 tgctcaaatc ttgcgtgatt tgcttgtttc tggtgcagat caagtgactg ttcaagctaa
    12181 gaaagaagac ctcttgcaag aaatctttaa ctttcttgct atgtcattag gacttccacc
    12241 acgcaagttt gactttgctt atcgcgataa agataacaac tacaaaagtg aaaaaggaat
    12301 cacaccacaa gagttctaca agaaatatgt caatcttcct ttagaagact acgtttctgt
    12361 tatcaatgct ccaactgctg ataaacctta cggaaaatct tacacagttg agatgttggg
    12421 gaatgtggtt ggtagccgtg cagttcgtta catcaacgtt ccaatggagc gcttgaaaga
    12481 attggcgatt gcccaaatgc aagcaggtga gactgtttgg tttggttctg atgtcggcca
    12541 gctcagtaac cgtaaggctg gaatccttgc gacagatgtt tatgactttg aatcaagcat
    12601 ggacattaaa cttactcaag acaaggctgg acgtttggac tatagcgaaa gcttgatgac
    12661 ccacgccatg gtcttgacag gtgttgactt ggacgaaaat ggtaaatcaa ccaagtggaa
    12721 ggttgaaaac tcatggggag acaaggtcgg tacagatggt tactttgttg cctcagacgc
    12781 ttggatggac gaatacacat accaaatcgt tgttcgtaag gaattgctga cagcagaaga
    12841 acaagctgcc tatggagcag aaccaattgt acttgcacca tgggatccaa tgggagcctt
    12901 ggctaaataa aagcatagaa aaaaggaatc agattttaga acctggttcc tttttagttg
    12961 cttgattaca tgatgtgaag aacatgtgcc acaataccca ctgcgaagag ggcaaggata
    13021 atagtgattg gagatacttt tttcttaagt aagtacatgc aaagtaaagt aaggagtagt
    13081 cctgataatc caggaatcaa catatccaag ttttgttgga aagtagtaac tttttcagga
    13141 gtttgagaca atccttgtcc tacttgtgcg aatgcttctt ggataccttt agacccttct
    13201 ggcaatttat cccaatggat ataagccttt tcatctagtt gaactttaga aacatcgaaa
    13261 gcaaatttaa tatttaccca gcgttgaaca aggacagcaa gaatgaacat cccaaggata
    13321 gaagctcctt tagtgatatc ttgaaggata ccaccagaca tatctttagt gatttctgat
    13381 ccagccttgt atccaatctc ttgaacatac cacaagaatg acatacgaat caagttccat
    13441 gcaacaaaga agaggagtgg ccccaagata ttgccagtaa gggcaagtga agcaccgaga
    13501 gatccaagga ttgggcgtac tgtaaaccag aatactgggt caccgatacc agcaagaggt
    13561 cccatcatac cgattttaac cccttggata gcagcgtcat cgatttccac accgttagca
    13621 cgttcttctt caagcgcaag agtaaccccc atgactggag cagctacgta tggatgagtg
    13681 ttgaagaact caaggtgacg ctcaagagca gcgatttgat cttctttttt agtatagagt
    13741 tttttgatag ctggaatgag tgtataagcc cagcccaagt tttgcatccg ttcaaagttc
    13801 caagaccctt gtaagaaggt tgaacgccac caaacttttt tacgatctga tttagttaat
    13861 tgaagttttt cagtcatgat gttttcagtc ctttcttatc ttagtagtct tctaggatat
    13921 cgccgattgg gtcgttagaa gttgcggctc ctccgccacc atttccacca gttttagaaa
    13981 ggtgaaggta gataagagcg atagcaacac cgatagcacc gaatccgatt agagtaatat
    14041 ctgacacagc agcgagaacg aaaccaagag cgaagaatgg ccatacttca cgagttgcca
    14101 tcatgttgat aaccatggcg taaccaacgg caacgaccat accaccaccg atagccatac
    14161 catctttgag ccagtctggc atggcactaa ggatactttg tacagtttca gttggtacca
    14221 taaggagaag agctgcagga agcgcgatac gaagtccttg gaagagtagc gcaatgaaat
    14281 gcgcacgctc cacagcgccg aagtcacctt ttttagcggc agcatctgca gtatgaacca
    14341 aaccaactga aattgtacga acaatcattg tcaagaaaag tccagctacg gcaagaggga
    14401 tagcaaccgc ttgggcaaca ccgataccag tcttggtaaa gtcaccacca agaaccatga
    14461 taatggcagc agcgacagaa gcaagtgcag catcaggagc gatagcagca ccgatatttg
    14521 accaaccaag ggcaatcatt tgaagcgatc caccgaggat aatccctgct tccaagtgac
    14581 ctgttacaag cccaataagg gtacaggcta caagtggttg gtgaaattgg aactggtcga
    14641 ggatgccttc aagacctgca aagaaggcta caacgactac taaaaccata gaaataatag
    14701 acatgtttaa aatcctttca taaataatgg cttatttgac attggctttg ttaatcaagt
    14761 caaacaaatc ttttttagaa tcatttggta ctttacggac atcaaattca acacccaagt
    14821 cacgcatttt ttcaaatgta gcaacatctt ctttgtccat agacaaaacg gtattgacca
    14881 atgttttacc tgttgagtga gccatagaac caacgttaag agtcttgatt ggcacgccgc
    14941 cttcgatggc acgaagggca tcttgaggtg tttcaaacaa gataagggca tgtgtttctc
    15001 caaaacgtgg gtcttttgaa atctcaatca gtttttgaat tggaaccacg ttagccttga
    15061 cattacctgg agctgcttgt ttaatcaatt ctttacgaag gtcgtcttta gccacgttat
    15121 ctgaagcaac gatgatacga tttgcttttg aatctggagt ccaagcagtt gcaacctgac
    15181 cgtgaagtag acgtgtgtca agacgggcaa gattgatttt caatttaccg tctccgataa
    15241 cagttccttc tgggatagca gtttgggcaa ctggagcagc tgcagcgctt gcaacttctt
    15301 caactggatt tagctcttct ggaagagctt tgatgccatc tttggcttct ttaatgatat
    15361 tagcagcgac tttttctaca cctgcagcag cgtccatgag gcgctctgtg taggcttgaa
    15421 tcaacatcgg taagttaagt cctgtgatga tggcaaactt acgctcagga ttttctccca
    15481 tcacgcgact agcttggtta aatggagaac cactccaaag gtcagccaaa actagaacct
    15541 catcttctgc gtcaaatgca gcaacagcgt tattaaactt agcgtataga tcatcaggac
    15601 cttcatttgg cataaaggtc acaacttgaa ccttttcttg ttcaccaaag atcatagatc
    15661 ctgactgatg aatacccgca gcaaattcgc cgtggctcgc aataatgatt ccgatactca
    15721 ttattgtcat tcctcctttt ttgttttagt tttcatttaa gaaaacttta agactgttta
    15781 agtataaacc gttttcataa aaaaagcaac tatttatcat aaaaaattaa aaaattttta
    15841 tttttgtatg ttgatatgtt gagtttttga ggagtaatct tgaattctta tttaaaattt
    15901 aatataaaaa agttggaaga agcttccaac ttttaaaata ggtttctatt agattagtgg
    15961 gtgaagtcga gtaccatacg tccttggatt tggccttttt ccatttcgtc gaaaatggca
    16021 acagcatctt ctactggacg tttttgaaca actgggacta ccagaccttc tgcaccaaat
    16081 tggaaggctt cttctaagtc tttacgagtt ccgacaagag aaccgatgac ttggattcca
    16141 tcgaggactg ttttaacgat gctgagttcc atcatttcag aaggaagacc aacagcgacg
    16201 acgcgaccac cagcacgaat ggagtcaaca gcctggttga aggcaacttt agacacagca
    16261 gttacgacag ctgaatgagc tcctccatca gttttttctt taatgagtcc agctacatct
    16321 tcaacttcga ggccgttaat cacaatgtca gcgcctactt cttttgcaag ggcaagtttg
    16381 tcattgttga tatcgactgc gataacatga gcattgaata cttttttagc gtattgaaca
    16441 gcgaggttac caagtccacc agcaccgtaa agaacaaccc attggcctgg ttcaactttt
    16501 gcttctttga tagctttata ggttgttact ccagcacatg tgatagaaga agcttgggct
    16561 ggatcaagtc cgtcaggaac tttgacagca tagtcagcag ttacgataca ttgttcagcc
    16621 ataccaccgt ctactgagta gccagcattt ttcactgtac ggcaaagggt ttcgcgacca
    16681 gttgtacagt attcgcaagt gccacatcct tcaaagaacc aagcaacgct gacgcggtca
    16741 ccgactttaa ggtttttcac atctggagca atctctttaa cgataccgat accttcgtgc
    16801 ccaagaacac gtcctgggac ttgaccaaag tcaccatgag caacgtggag gtcggtgtgg
    16861 caaacgccac agtattcaac ttctacaagt gcttccccag tttcaagtgg acggagtact
    16921 ttttcttcaa tagcaacacc agtgctttct ggatttacaa caacagcttt catagctatc
    16981 ctccttgata ttgaccgaga acagggacaa cgggactgga ttgatttttt gtcaacagag
    17041 tcgagtgtga cgagctctct tgtatttata tgtgaagtat atcacaaaag aaagcctttt
    17101 ccaaggtttt ggagacaaaa agtagaacaa tcgattaact ggttgatgag actgtgaaaa
    17161 gagcacaaag accagcttgc aagctggtca tactcttcga aaatctcttc aaaccacgtc
    17221 agcttcatct acaacctcaa agcagtgctt tgagcaacct acggctagct tcctagtttg
    17281 ctctttgatt ctcattgagt atcagtatag gttataagaa taagtcttgt agggttttgg
    17341 caaccccatc ttggtcgttg gtaagggaaa tttgctcatc tgcataaggg agtagctctg
    17401 gattggcatt tttcatggca taacccttcc cagcaaaagc gagcatttcg gtatcattgt
    17461 gttcatctcc aaaggcaatc aaatcttttt tgtcacgatt cattatcttg agcaagtagt
    17521 ccaaagcaaa ggccttgttg acaccttttg gggtacattc aaggatattg agcggacctc
    17581 cccaggtatt gatagaaagt tgatgctggt agaaggcgtt catttctttt gccaaggcat
    17641 atttgtcact ggctctggtc tgcaagagga tacagttagg gtccttggtc accaattcag
    17701 gctggaattg atcttcaggc tggaaagctt ctacaccaaa tagtttggga ttggcaattt
    17761 cttcattggg atttgtaatg tagaattttt tacgatattc tccagcgata aaatcggctt
    17821 gaatgtcctc tgaacgttga accatatcta gcagatattt tttgtctaca gtcaaacact
    17881 tttcaaaatc ccaaacttgg tctggtaaat gagtaaggga tccgttgaag ttaatcatag
    17941 gagtgtctaa gcccagttca cggtaaaaat cttttgacat acggtaaggg cgacctgtcg
    18001 taataataac ctgatggcct ttttcagcaa cttttttgat ggtttttttg gtaaagtcag
    18061 aaatttgact gtctgagttg agcagggttc catctaggtc aactgcaata atttttttcg
    18121 tcatatggac ctttctagtg tgttttcaga taagatgtct tctattataa cagaaagcag
    18181 gcagaaaagc agattccaaa caaaaaatga aattggatta atttcttaaa caaatcaaac
    18241 aaagacattg aaaaagtgaa tgataaatga tataattcta ttattgttcg taaaaattaa
    18301 aaggagattg atgatggaca aattatttaa actaaaagag aacggtacag acgttcgtac
    18361 agaggttctc gctggtttaa caactttctt tgcaatgagc tatattctct ttgtaaaccc
    18421 acaaatactt tcacaaacag gaatgcctgc tcagggtgtg tttcttgcaa cgattatcgg
    18481 tgcagttgct ggtaccttga cgatggcctt ctatgctaac ttgccatacg ctcaagcacc
    18541 aggtatggga ctcaatgcct tctttacctt tacagtcgta tttggacttg gttattcttg
    18601 gcaagaagcc ctagctatgg tcttcatctg tgggattatt tcattgatta ttaccttgac
    18661 aaatgttcgt aaaatgatca ttgaatcgat tcccaatgct cttcgctcag ctatttcagc
    18721 tggtatcggt gtcttccttg cctatgtagg gattaagaat gctggacttt tgaaattcac
    18781 gattgatcca ggcaactata ctgttgtagg agaaggggct gacaaagctc aagcaacgat
    18841 tgcagcaaac tcttcagcag ttccaggatt ggtcagcttt aataatccag ctgttttagt
    18901 ggctcttgca ggacttgcca ttactatctt ctttgtcatc aaagggatta aagggggaat
    18961 tattctctct atcttgacaa caactgttct tgctattgca gttggtttgg ttgatttgtc
    19021 tagtatcgat tttgctaata accatgttgg tgcagctttt gaagatttga agacaatctt
    19081 tggtgcagct cttggttcag aaggtttggg agctttggtt tcagatacag ctcgcttgcc
    19141 tgaaactctg atggccattc ttgccttctc attgacagat atttttgaca caattggtac
    19201 cttgatcggt acaggtgaaa aagttggtat tgtagcgaca aatgatgaaa atcaccaatc
    19261 agccaaattg gataaggctc tttactctga tttgattgga acgacagtcg gtgccattgc
    19321 aggtacttca aacgtaacga cttatgttga gtctgctgct ggtatcggtg caggtggacg
    19381 tactggtttg acagccttgg ttgtagctat ctgttttgcg atttcaagct tctttagccc
    19441 acttctagcg atcgtaccaa cagcggctac agctccaatc ttgattatcg ttgggattat
    19501 gatgcttggt agcttgaaaa atatccattg ggatgatatg tctgaagcag ttcctgcctt
    19561 cttcacatct atctttatgg gattcagcta ctctatcact caagggattg cagttggttt
    19621 cttgacttac actttgacta agcttgtcaa aggtcaagtt aaagatgttc atgccatgat
    19681 ttggattttg gatgccttgt ttatccttaa ctacatcagc atggccttat aatagaatga
    19741 cccaggggga tttccccctt tttttaatac aaaggagata ggtgatgaaa gagaaaaata
    19801 tgtggaaaga attgttgaat cgtgcaggct ggattttggt ctttttactt gccgtccttt
    19861 tatatcaggt tcccctagtg gttacctcta ttttgacttt aaaagaagta gccctgctac
    19921 agtcagggct gatagttgct ggcctttcaa ttgtggttct ggctctattt attatgggag
    19981 ctcgtaaaac caagttagct agttttaatt tttctttttt tagagctaaa gatttggcac
    20041 gtttggtctt gagttattta gtcattatct gttcaaatat acttggttct atcttgttgc
    20101 aactgtcaaa tgagacgaca acagctaacc agtctcagat taacgatatg gttcagaata
    20161 gttcgttgat ttctagcttc ttcttgctag ccttgcttgc tccaatttgt gaggaaatct
    20221 tgtgccgtgg gattgttcct aaaaagattt tccgaggcaa ggagaacttg ggatttgtag
    20281 tcggtacgat tgtgtttgct ttattgcatc aaccaagtaa tttaccttct ttattgattt
    20341 atggaggtat gtcgacagtt ctatcttgga cggcctacaa gacccaacgt ttggaaatgt
    20401 cgatcttgct tcacatgatt gttaatggga ttgctttctg tttgttggct cttgtggtga
    20461 ttatgagtcg gacattagga atttctgtgt aaaagttttt atgtaggaac
//
LOCUS       DAVBCR010000033        20487 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCR010000033 DAVBCR010000000
VERSION     DAVBCR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.033"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(88..2631)
                     /locus_tag="VQ508_001135"
     CDS             complement(88..2631)
                     /locus_tag="VQ508_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2490141.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2693..4315)
                     /locus_tag="VQ508_001136"
     CDS             complement(2693..4315)
                     /locus_tag="VQ508_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490142.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4521..5546
                     /gene="trpS"
                     /locus_tag="VQ508_001137"
     CDS             4521..5546
                     /gene="trpS"
                     /locus_tag="VQ508_001137"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2490143.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5699..7177
                     /gene="guaB"
                     /locus_tag="VQ508_001138"
     CDS             5699..7177
                     /gene="guaB"
                     /locus_tag="VQ508_001138"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2490144.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7228..8325)
                     /gene="recF"
                     /locus_tag="VQ508_001139"
     CDS             complement(7228..8325)
                     /gene="recF"
                     /locus_tag="VQ508_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2490145.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8328..8696)
                     /locus_tag="VQ508_001140"
     CDS             complement(8328..8696)
                     /locus_tag="VQ508_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2490146.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8855..10105
                     /locus_tag="VQ508_001141"
     CDS             8855..10105
                     /locus_tag="VQ508_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2490147.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLFDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10102..11385
                     /locus_tag="VQ508_001142"
     CDS             10102..11385
                     /locus_tag="VQ508_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2490148.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11427..12248
                     /locus_tag="VQ508_001143"
     CDS             11427..12248
                     /locus_tag="VQ508_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2490149.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12259..12804
                     /gene="pgsA"
                     /locus_tag="VQ508_001144"
     CDS             12259..12804
                     /gene="pgsA"
                     /locus_tag="VQ508_001144"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2490150.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIILIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12801..13628
                     /locus_tag="VQ508_001145"
     CDS             12801..13628
                     /locus_tag="VQ508_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2490151.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13613..14452
                     /locus_tag="VQ508_001146"
     CDS             13613..14452
                     /locus_tag="VQ508_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2490152.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14445..15239
                     /locus_tag="VQ508_001147"
     CDS             14445..15239
                     /locus_tag="VQ508_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2490153.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15298..16116
                     /gene="mreC"
                     /locus_tag="VQ508_001148"
     CDS             15298..16116
                     /gene="mreC"
                     /locus_tag="VQ508_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2490154.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTVVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16116..16610
                     /gene="mreD"
                     /locus_tag="VQ508_001149"
     CDS             16116..16610
                     /gene="mreD"
                     /locus_tag="VQ508_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2490155.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16704..17882
                     /locus_tag="VQ508_001150"
     CDS             16704..17882
                     /locus_tag="VQ508_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2490156.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18106..18885
                     /gene="rpsB"
                     /locus_tag="VQ508_001151"
     CDS             18106..18885
                     /gene="rpsB"
                     /locus_tag="VQ508_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2490157.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            18963..20003
                     /locus_tag="VQ508_001152"
     CDS             18963..20003
                     /locus_tag="VQ508_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2490158.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20249..20487
                     /gene="tnpB"
                     /locus_tag="VQ508_001153"
                     /pseudo
     CDS             20249..20487
                     /gene="tnpB"
                     /locus_tag="VQ508_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         6509 a         3274 c         4244 g         6460 t
ORIGIN      
        1 acttagccct gtgcaataca gaactaaatc cttcggataa attaattgtc taactttttg
       61 gggtcagtac attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata
      121 gattccaaat agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg
      181 aatgaaggaa agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt
      241 ttgagcttgt ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata
      301 aggaatggtg aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt
      361 tttagaagtt gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt
      421 tttggtatct aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa
      481 actaacattg atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa
      541 gacattatca gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg
      601 tttatcgtta gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac
      661 ttcaatttga tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc
      721 attagtattt tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc
      781 aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt
      841 gtaaacagat tgactagcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag
      901 attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt
      961 gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag
     1021 attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg
     1081 aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg
     1141 agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat
     1201 agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc
     1261 gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat
     1321 ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac
     1381 aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt
     1441 tagatagatg gtagctaaaa atcctgctac tacaagaaaa agcgaaacta aaaaattcca
     1501 gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga
     1561 gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa
     1621 gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag
     1681 tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa
     1741 tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa
     1801 ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt
     1861 caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa tcaaactagc
     1921 cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa
     1981 gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa
     2041 ataataattt tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc
     2101 agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca
     2161 ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc
     2221 tagttttaaa gtctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag
     2281 accaatcaat ccaaatttta agagagtggt cagatagaca gcatctggca tattcgttag
     2341 atcaaaaaag taaactagag gcgcgagaaa actacccaag taataactag atagggcata
     2401 gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ctactatttc catgtaggat
     2461 atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga
     2521 gttgtcgcta ttccagtaga taccttgaga tagatatact ccagacataa tcactacagg
     2581 aatgatgaaa gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg
     2641 tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt
     2701 tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt
     2761 catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt
     2821 gaataaactc gtggtcatgg ctggcaaaga tgattgattc tttaaagttt ttcagtccat
     2881 cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga
     2941 catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca
     3001 agacatttac aggtttgtta acttcatctc cagagaagag catacggccg aggaaaccac
     3061 gtaggaaagt attgtcatct tcttctttac ttgcgaattg acgcaaccag tcaagaattg
     3121 attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga ctagttgtaa
     3181 ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag
     3241 tcgtttgaat atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac
     3301 taatattatc caagatagtt tcaccatcaa tctttacagt tagattttct actgtcaaga
     3361 gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg
     3421 gtacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg
     3481 acttagaagc attggcagag aaacgagcaa cgaattcttg caattgttta attttttctt
     3541 ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga
     3601 agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac
     3661 aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa
     3721 tcaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca
     3781 agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac
     3841 cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa
     3901 gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga
     3961 gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt
     4021 tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt
     4081 catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag
     4141 agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc
     4201 cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat
     4261 catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatgttt
     4321 ttctcctata tgtgtaatat atttattcta ctagaaaata cagaaatatt caaattttta
     4381 tttgtcaatt ttgtgtaaat tatatttaca gtatccttta cacaaatctg taaaaagcaa
     4441 ggctgattta ttttgataaa ttacggttat ttcattaaaa aaatgctata attgaaagga
     4501 ctatatcgaa ggagaacaaa atgactaaac ccattatttt aacaggagac cgtccaacag
     4561 gaaaattgca tattggacat tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag
     4621 aggataagta tgatatgttt gtgttcttgg ctgaccaaca agccttgaca gatcatgcca
     4681 aagatcctca aaccattgta gagtctatcg gaaatgtggc tttggattat cttgcagttg
     4741 gattggatcc aaataagtca actattttta ttcaaagcca gattccagag ttggctgagt
     4801 tgtctatgta ttatatgaat ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca
     4861 agacagagat ttctcagaaa ggatttggag aaagcattcc gacaggattc ttggtctatc
     4921 caatcgctca agcagctgat atcacagctt tcaaggctaa ttatgttcct gttgggacag
     4981 atcagaaacc aatgattgag caaactcgtg aaattgttcg ttcttttaac aatgcatata
     5041 actgtgatgt cttggtagag ccggaaggta tttatccaga aaatgagaga gcagggcgtt
     5101 tgcctggttt agatggaaat gctaaaatgt ctaaatcact aaataatggt atttatttag
     5161 ctgatgatgc ggatactttg cgtaaaaaag taatgagtat gtatacagat ccagatcata
     5221 tccgcgttga ggatccaggt aagattgagg gaaatatggt tttccattat ctagatgttt
     5281 ttggtcgtcc agaagatgct caagaaattg ctgatatgaa agaacgttat caacgaggtg
     5341 gtcttggtga tgtgaagacc aagcgttatc tacttgaaat attagaacgt gaactgggtc
     5401 ctattcgtga gcgccgtatt gaatttgcta aggatatggg agaagtttat aatatgattc
     5461 aaaaaggtag tgaaagagcg cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg
     5521 caatgggact tcattacttt aactaagttt attttacaag aaactatcat ttctatctcg
     5581 aagaaaagat agttttttta tttacccctg taacatttga caaattttta tagaatggta
     5641 tgatagatac aatataaaaa gagtcaagct caaaaagaaa gaaaagagga aacttcgaat
     5701 gtctaattgg gacactaaat ttttgaaaaa aggttttacc tttgatgatg tattgcttat
     5761 tccagctgaa agtcatgtgt tgcctaacga tgcagattta acaactaaat tggcagataa
     5821 tttgacttta aatatcccaa ttattaccgc tgccatggac acagttacag agagtcaaat
     5881 ggccattgct attgctcgtg caggcggtct cggagttatc cataaaaaca tgtcaattgc
     5941 tcaacaagca gacgaggttc gtaaggtaaa acgttctgaa aatggagtta ttattgatcc
     6001 gttcttcttg acgcctgaac atacaattgc tgaagcagat gagcttatgg gtcgttaccg
     6061 catcagtggt gttccagttg ttgaaacact tgaaaatcgt aaattggttg gtattttgac
     6121 aaaccgagat cttcgtttta tttcagatta taatcaacca atttcaaacc atatgactag
     6181 tgaaaatctt gttactgctc ctgtgggtac ggatcttgca acggctgaga gtattcttca
     6241 agagcatcgt attgaaaaac ttccgttggt cgatgaagaa ggcagtcttt ctggtttgat
     6301 cactatcaaa gatattgaaa aagttattga gtttccaaat gcggctaaag atgagtttgg
     6361 tcgtcttcta gttgcaggtg cagtaggtgt tacttcagat acatttgaac gtgcagaggc
     6421 tctttttgag gcaggagcgg atgcgattgt tattgatact gcacatggtc attctgcagg
     6481 tgtcttgcgt aaaattgccg agattcgtgc tcatttccca gatcggactt tgattgctgg
     6541 aaatattgct actgctgaag gtgcacgtgc cctttatgaa gcgggtgtag acgttgttaa
     6601 ggttggtatt ggaccaggtt ctatctgtac tactcgtgtg attgctggtg ttggtgttcc
     6661 gcaagtaaca gctatctacg atgctgcagc tgttgcgcgc gaatatggta aaacgattat
     6721 tgctgacggt gggatcaagt attctggaga tattgtaaaa gcacttgctg caggtggaaa
     6781 tgctgttatg cttggatcta tgtttgctgg aactgatgaa gctccaggcg aaactgaaat
     6841 cttccaagga cgtaaattca agacttaccg tggtatggga tcaattgctg ctatgaagaa
     6901 aggttcaagc gaccgttatt tccaaggttc tgtcaatgaa gcaaacaagc ttgttccaga
     6961 aggaattgaa ggtcgtgttg cttataaagg agcggcagct gatattgttt tccaaatgat
     7021 tggtggtatt cgctctggta tgggttactg tggtgcagct aaccttaaag aactacacga
     7081 taatgctcaa tttattgaaa tgtctggtgc tggtttgaaa gaaagccatc ctcatgatgt
     7141 gcaaattact aatgaggcac caaattattc tatgtaaaaa acaatgaaaa gaactccagt
     7201 gaaaacagga gttcttttac aatgttgtca atttccattt acagcagctt taccatcctg
     7261 aatagtgaag atacttagat tttctggcag attttgaaga tggtctaagc ttgttgttgt
     7321 gataaaggtt tggattgatt gagaaatcgt ttctaataat tttaactgtc tagtgttgtc
     7381 aagttcactc atcacatcgt caagcagtaa tataggagat tctgtggtaa tgctttccat
     7441 taattcgatt tctgctaatt ttatcgagag gacgagacta cgatgttgac cttggcttcc
     7501 gaaactagca tccatcccat ttatataaaa agaaatgtca tctcgatgag gaccgacacc
     7561 agtattcttt ttaaataaat ctctggatct acttttttct aaagcaattt tgaaagattc
     7621 ggataagttt tgtttgtcag ttatattgac agaagattga taggatattg acaactcttc
     7681 gatctgatta gagagttcaa aatgtttctt acgcccaaat gattctagtt tttttatgaa
     7741 atctaagcgg tgattcatta cacgacatcc ataatcaact agctgatcat ctaacacaga
     7801 aaggaatgtt tcatctattt tttgagctga ttttaggtaa gtgtttcttt gctttaggat
     7861 gtggttataa ttggttaagt cagataaata gattggctta atttgcccaa gttccatatc
     7921 aatgaatttt cgtcgaatcg aaggtgctcc tttaattagt tgtaaatctt caggagcaaa
     7981 taagacaaca ttcatgtgtc ctacataatc tgaaaggcgt gcctgtttta agtgattaac
     8041 ttttgttaca cggccttttt gtgttagttc gatttctagg ggaatggatc cagttttttt
     8101 ctgaacgaga cctgaaagat gaagttgttc ctcatcaaaa tgaatgagat ttttatctgt
     8161 tcgagttcga tgactacgcg ttaaggctaa aaaatagata gcctctaaca tgtttgtttt
     8221 tccttgtgca ttacgtccca aaaagacatt caatttagga ttaaagtcta ttttcgtttc
     8281 tttgtaatta cgaaaagtct tgagagatag atgttgtagc cacatgatta tctaccaggg
     8341 aatcgtggag cttgttggct tttaggtgat gaagcaggtt tcgatttgtc ttttttaact
     8401 cccttattca tctttttgac aagtttagcg atccgttctt tttcaacttt atcagcttgg
     8461 tattcatctt gttcttcaga agtaggttgt gtcaacaaga tgtcaatgtt catgtcaggg
     8521 atgtcaactt tatcgccaat acgaagtttt tttccacgac aactctctaa ttccctatta
     8581 aagtaaacag aatgttcaga gagaaatgat ttaatagctc ctccgctatg tgtaattcca
     8641 agttctttga gtagtgcttg gagggtaata aattcttcaa ataatttgta ttccataatt
     8701 cacctcaatc tttggtatta taccatattt tcctaaaaat agtgagagag acagctagaa
     8761 atgtaagcga tttttatttc aaaagtgtta cagggaacaa gaaaatttca ggttttcgtg
     8821 atataataga agtctgtata taaggaggta aatcatggag ttagtgcatg gaatttcaac
     8881 acattttatc caatcaaaaa agtttaaaac aaacaaaatt accgtgcgtt ttaccgctcc
     8941 attatccctt gatacgattg caggtcacat gttgagtgca agtatgctag agactgctaa
     9001 tcagatgtac cccacttctc aagatttgag gagacacttg gccagtctat acggtacaga
     9061 tatgtcaacc aattgtttca gaagagggca aagccacatt atagaattga catttaccta
     9121 tgttcgtgat gagtttttaa gtaggaaaaa cgtgctaacc tctcagattt tggaacttgt
     9181 aaaagaaact cttttttcac ccgcagtagt tgataatggg tttgatccgg ccttatttga
     9241 aattgagaaa aaacaattgc tagcaagttt agcagctgat atggatgatt ctttttattt
     9301 tgcacataaa gaattggata aattgttttt tcatgatgaa cgtcttcaat tggaatatag
     9361 tgatttacga aatcgtattt tagctgaaac tccacaaagt tcttattctt gtttccaaga
     9421 atttttagcc aatgatcgaa tagatttctt tttcctaggt gattttaatg aggttgaaat
     9481 tcaaaatgta ttagaatcat ttggctttaa aggtcgaaaa ggagatgtga aggttcagta
     9541 ttgtcaacct tattctaata tccttcagga aggtatggtt cggaaaaatg tgggacaatc
     9601 cattttggaa ttaggttatc attaccgttc taaatatggt gatgaacaac atttacccat
     9661 gattgtaatg aatggtttac ttggtggatt tgctcactct aagctcttta caaatgtccg
     9721 tgaaaatgct ggattagctt ataccatttc aagtgagctt gatttattta gtggattctt
     9781 gaggatgtat gctggtatca atcgagaaaa tcgtaaccag gctcgtaaaa tgatgaataa
     9841 tcaactgttt gatttaaaaa aaggttattt tacagagttt gagttaaatc agaccaagga
     9901 aatgattcgt tggtcgttgt tactttctca agataatcaa tcttcattga ttgaacgtgc
     9961 ttatcaaaat gccttatttg gaaaatcttc agcagacttt aaaggttgga ttgcaaaact
    10021 cgagcaagtt gacaaagatg ctatttgtag agcagctaat aatgtgaaac tacaggcgat
    10081 ttactttatg gaaggaatag aatgacaaag gttgtttttg aaaaaaaata ctatccagct
    10141 gtaaaagaaa tgatttatcg aactcgtttg gccaacggat tgacagttgc tcttttgcct
    10201 aaaaaggaat ttaaagaggt ttacgggagt gtcactgtac agtttggttc ggtagatacg
    10261 tttgtcacag aagttgacgg agatgtaaaa caatatcctg gaggaattgc tcattttctt
    10321 gaacataaat tatttgagag agaagattct agtgatttga tgtcggcttt tacgagtcta
    10381 ggtgcagata gtaatgcctt tacaagcttt acaaaaacaa actatctttt ttcagcaacg
    10441 gattattttt tagaaaattt agatttactt gatgaattgg taacatcagc acactttact
    10501 gaagcttcca ttctgacaga gcaggatatt attcagcaag aacgagaaat gtaccaagat
    10561 gatccagatt cgtgtttatt cttttcaact ttagcgaatt tgtatcctgg tacaccttta
    10621 gcaactgata tagttggaag tgaggagtcc atttcccaaa taaatctaac taatttgcaa
    10681 gaaaatttta caaagtttta caaacctgta aacatgtctc tgtttttagt tggtaatttt
    10741 gatgtggagc gagtacagga ctattttgaa agcaaagaac tgaaagattc agattttcag
    10801 gaagtagcaa gagaaaagtt gtttttacag cctgtaaagc caacagatag tatgagaatg
    10861 gaagtatctt ctcccaaact agcgattgga gttagaggta agcgagaagt ttctgaagcg
    10921 gattgctatc gacatcatat tttattaaaa ttattgtttg caatgatgtt tggttggact
    10981 tcggatcgtt ttcaaaaatg ttatgaatca ggtaaaattg atgcgtcctt atctctggaa
    11041 gttgaaataa caagtcgctt tcattttgtc atgttgacaa tggatacgaa agagccagtt
    11101 gctttgtctc atcaatttag gaaggctatt cgtaatttta caaaggattt agatattaca
    11161 gaggaacatt tagatattat caaaagagag atgtttggcg aatttttcag tagcatgaac
    11221 tctcttgaat ttattgcaac gcaatatgat gcttttgaaa atggtgagac aatttttgat
    11281 ttgccgaaaa ttttacagga aattacttta gaggatgtcc ttgatgctgg acatcattta
    11341 atagatgatg gtgacatagt tgattttaca atatttccat cgtagtaacc tatcataata
    11401 gacactagaa agaagggatg acaagtatga gaaaaaaaac aattggagag gttttacgat
    11461 tagctagaat caatcaggga ttgagtttag atgaattgca gaaaaagaca gaaatccagt
    11521 tagatatgtt ggaagcaatg gaagcagacg atttcgatca acttccaagt cctttttaca
    11581 cgcgttcttt cttgaaaaaa tatgcatggg ctgttgagtt agatgaccaa attgttttgg
    11641 atgcttatga ttctgggagt atgattactt atgaggaagt agatgttgat gaagatgagt
    11701 tgacaggtcg tagacgttca agtaagaaaa agaagaaaaa aacatcattt ttacctttat
    11761 tttattttat cctttttgct ttatcgattt taatttttgt gacttattat gtttggaact
    11821 atattcaaac tcaaccagag gagccttctc tttctaatta cagtgtggtt caatcaacaa
    11881 gttcaactag ctctgttccc cactcctcaa gtagtagttc ttctagtata gaatcagcta
    11941 taagtgtatc aggcgaagga aatcatgtag aaatcgctta taagacaagt aaggaaacag
    12001 ttaaattgca attggcagtt tcagatgtta caagttgggt cagtgtttca gaaagcgaac
    12061 ttgagggcgg tgtaacctta tcgccaaaga agaaaagtgc agaagcaaca gttgcaacta
    12121 aaagtcctgt aacaattacg ttaggtgttg taaaaggtgt tgatttgaca gtagataatc
    12181 agactgttga tttatcgaaa ttaacagctc agactggaca aatcactgta acctttacta
    12241 aaaattaagg aaaaacgaat gaaaaaagaa caaattccca atctcttaac aataggtcga
    12301 attctcttta tacctatttt tatctttatt ttaacgatag gaaattcgat agagagtcat
    12361 atagttgcag ctattatctt tgctgttgcc agtattaccg actatttaga tggatattta
    12421 gctcgtaaat ggaatgtggt cagtaatttt ggtaaatttg cagatcctat ggcggataag
    12481 ttactagtta tgtcggcttt tattatcttg attgagttag gtatggctcc ggcttggatt
    12541 gttgcagtga ttatctgtcg tgagttagct gtgacaggtt taaggctttt attggttgaa
    12601 actggtggaa caattttagc agcagcaatg cctggaaaaa ttaaaacttt tagtcagatg
    12661 tttgccatta ttttcttgct attacattgg actttgcttg gtcaagttct actttatgta
    12721 gccttatttt tcactatcta ctctggctat gactatttca agggtagtgc ctatgtattt
    12781 aaagggacat ttggttcgaa atgaaatcaa taattgatgt aaaaaatctt tcttttcgtt
    12841 ataaagaaaa tcagaactac tacgatgtga aggatattac gtttcacgtg aaacgtggag
    12901 aatggctttc gattgtaggg cataatggta gtggtaaatc aacgacggtt cgattaattg
    12961 atggcttact ggaagcagaa tctggagaga ttgtaattga tggccaacgt ttgactgagg
    13021 aaaatgtttg gaatatacgt cgtcaaatcg gtatggtttt tcaaaatcca gacaatcaat
    13081 ttgttggagc gactgttgaa gatgatgttg cttttggttt ggaaaatcag ggactttctc
    13141 gtcaagaaat gaaaaagaga gtggaagaag ctctggcttt agttggcatg ttggacttta
    13201 aaaagagaga gccagcgcgt ctatcaggtg gccaaaagca acgtgtggcc attgcaggtg
    13261 ttgtagccct aagaccagct attttaatct tagatgaagc aacgagtatg ttggatcctg
    13321 aggggcgtag agaacttatt gggacagtaa aaggaattcg aaaagactat gatatgacgg
    13381 tcatttctat tacccatgat ttggaagaag tcgccatgag tgatcgcgta ttggtcatga
    13441 aaaaagggga aattgaatca actagtagtc caagggagct tttctctcga aatgatttag
    13501 atcaaattgg attagacgat ccttttgcta atcaattaaa aaaatctttg agccagaatg
    13561 gctatgattt acctgaaaat tatttgacag aaagtgagct agaggataag ctatgggaat
    13621 tgctctagaa aatgtgaatt ttacatatca agaaggtact cccttagctt cagcagcttt
    13681 gtcggatgtt tctttgacga ttgaagatgg ctcttataca gctttaattg ggcacacagg
    13741 tagtggtaaa tcaactattt tacaactctt aaatggttta ttggtgccaa gtcaagggag
    13801 tgtgagggtt tttgatacct taatcacctc gacttctaaa aataaagata ttcgtcaaat
    13861 tagaaaacag gttggcttgg tatttcagtt tgctgaaaat cagatttttg aagaaacggt
    13921 tttgaaggac gttgcttttg gaccgcaaaa ttttggagtt tctgaagaag atgctgtgaa
    13981 gactgcgcgt gagaaactgg ctctggttgg aattgatgaa tcactttttg atcgtagtcc
    14041 gtttgagctg tcagggggac aaatgagacg tgttgccatt gcaggcatac ttgccatgga
    14101 gccagctata ttagtcttag atgagccaac agctggtcta gatcctctag ggagaaaaga
    14161 gttgatgacc ctgttcaaaa aactccacca gtcagggatg accatcgtct tggtaacgca
    14221 tttgatggat gatgttgctg aatatgcaaa tcaagtctat gtaatggaaa agggacgttt
    14281 agtaaagggg ggcaaaccaa gtgatgtctt tcaagacgtt gtttttatgg aagaagttca
    14341 gttgggagta cctaaaatta cggccttttg taaacgattg gctgatagag gcgtgtcatt
    14401 taaacgatta ccgattaaga tagaggagtt caaggagtcg ctaaatggat agtatgattt
    14461 tggggcgtta tatcccaggg gattcgattg ttcaccgatt ggatccacgt agcaaattgc
    14521 ttgctatgat gctactgatt ttgattgtgt tttgggctaa taaccccttg acgaatctga
    14581 ttctttttat agcgacaggg atatttattg ccttgtcagg agtatctctt tcatttttta
    14641 ttcagggctt gaagtctatg tttttcttga ttgccttcac aactattttt caactatttt
    14701 tcatttctaa tgggaatgtt ttatttgagt tttcgtttgt gagaatcacg gattatgctt
    14761 tgcaacaagc tgggattatt ttttgtcgct ttgtattgat tattttcttt tcaactttgt
    14821 taaccttaac aaccatgcct ttaagtttgg catcagctgt cgaagcttta ttagcacctt
    14881 taaagcgtgt gaaagttcca gttcatgaaa ttggattgat gctgtctatg agtttgcgtt
    14941 ttgtcccaac cttgatggat gatacgactc ggattatgaa tgcacagaaa gctcgtggag
    15001 tggattttgg agaaggaagc atcgttcaaa aagtaaaggc gatgattccc attttgattc
    15061 ctctttttgc gacaagttta aaacgtgcag attccttggc tatcgctatg gaagcgcgtg
    15121 gctatcaggg tggaaaaggc agaagtcaat accgacaatt gaaatggact ctaaaggata
    15181 cgctgaccat tcttgttatt ctcgtacttg gttgttgttt atttttctta aaatcttagt
    15241 agctttcagg aattgataaa aagttactgt aacagttttt gatataaagg taggaatatg
    15301 aaccgtttta aaaaatcaaa atatgtcatt attgtttttg tcactgttct gcttgtgtca
    15361 gctctcttag cgacgactta ttcaagtaca gttgtgacaa aattaggaga tggaatctca
    15421 ttggttgata gagttgtaca aaaacctttt cagtggtttg attctgtcaa atcagatttg
    15481 gctcatttga cacgaacata taatgaaaat gaaagtttga agaaacagct ttaccaatta
    15541 gaagttaaat caaatgaggt ggaaagttta aagacagaaa atgaacaact gcgccaattg
    15601 cttgatatga agtctaaatt gcaagccaca aagactttag cagcagatgt tattatgcgt
    15661 tctccggtat cttggaagca ggagttgacc ttagatgcag gtagatcaaa aggtgcttct
    15721 gagaacatgt tagctattgc aaatggtggc ttgattggga gtgtttcaaa agtagaggag
    15781 aactctacta tagtcaacct tctgacaaat acggaaaatg ctgataagat ttctgttaaa
    15841 attcaacatg gctctactac aatttatgga attattattg gctatgacaa ggaaaatgac
    15901 gttcttaaaa ttagccaatt aaatagtaat agcgatatta gtgcgggaga taaggtgact
    15961 actggtggat taggaaactt taacgttgct gatattcctg ttggtgaagt ggttgccaca
    16021 acgcatagta cagactattt gacacgagaa gtaactgtta aattgagtgc agatactcat
    16081 aatgtagatg tgatagaatt agtggggaat tcataatgag acagttgaag cgagttggag
    16141 tatttttatt gcttcctttc tttgttctaa ttgacgccca tattagccag cttctgggct
    16201 catttttccc ccatgtacat ttggctagtc attttctttt tctatttctc ttatttgaga
    16261 cgatagaagt atcagagtat ctctacctag tctattgttt tgttataggc ttggtttatg
    16321 atgtttactt tttccatcta atagggatta caactctctt atttatctta ttgggagcct
    16381 tccttcataa attgaatagt gttattttgt tgaatcgttg gacaagaatg ctagctatga
    16441 ttgtgctgac attcctgttt gaaatgggta gttatctttt ggcttttatg gtagggttga
    16501 cagtagatag catgtcgatt tttatagtct atagcttggt accgacgatg attttaaatt
    16561 ttttatggat tactgttttt caatttattt ttgaaaaata ttatctataa gaacgacata
    16621 taaatgtaac aaaggcgtaa tatttattag gccttttttt ggtatactag tattgtcttt
    16681 aaaagaagga gtatctacgt aatatgaaga aaaaaatctt agcgtcactt ttattaagta
    16741 cagtaatggt ttctcaagta gctgttttaa caactgcgca tgcagaaacg actgatgaca
    16801 aaattgctgc tcaagataat aaaattagta acttaacagc acaacaacaa gaagcccaaa
    16861 aacaagttga ccaaattcag gagcaagtat cagctattca agctgagcag tctaacttgc
    16921 aagctgaaaa tgatagatta caagcagaat ctaagaaact cgagggtgag attacagaac
    16981 tttctaaaaa cattgtttct cgtaaccaat cgttggaaaa acaagctcgt agtgctcaaa
    17041 caaatggagc cgtaactagc tatatcaata ccattgtaaa ctcaaaatca attacagaag
    17101 ctatttcacg tgttgctgca atgagtgaaa tcgtatctgc aaacaacaaa atgttagaac
    17161 aacaaaaggc agataaaaaa gctatttctg aaaaacaagt agcaaataat gatgctatca
    17221 atactgtaat tgctaatcaa caaaaattgg ctgatgatgc tcaagcattg actacgaaac
    17281 aggcagaact aaaagctgct gaattaagtc ttgctgctga gaaagcgaca gctgaagggg
    17341 aaaaagcaag tctattagag caaaaagcag cagctgaggc agaggctcgt gcagctgcgg
    17401 tagcagaagc agcttataaa gaaaaacgag ctagccaaca acaatcagta cttgcttcag
    17461 caaacactaa cttaacagct caagtgcaag cagtatctga atctgcagca gcacctgtcc
    17521 gtgcaaaagt tcgtccaaca tatagtacaa acgcttcaag ttatccaatt ggagaatgta
    17581 catggggagt aaaaacattg gcaccttggg ctggagacta ctggggtaat ggagcacagt
    17641 gggctacaag tgcagcagca gcaggtttcc gtacaggttc aacacctcaa gttggagcaa
    17701 ttgcatgttg gaatgatggt ggatatggtc acgtagcggt tgttacagct gttgaatcaa
    17761 caacacgtat ccaagtatca gaatcaaatt atgcaggtaa tcgtacaatt ggaaatcacc
    17821 gtggatggtt caatccaaca acaacttctg aaggttttgt tacatatatt tatgcagatt
    17881 aatttacaga gggactcgaa tagagccctc ttttcaggtt ttaccgtgac aatccctatt
    17941 aaaaattata tcaaaatagc ttgaaaatat tggaaaagta tggtagaatg aaaattgtcg
    18001 tgtgaacgat aatactcatt cttgatgaat tgtgaagcag ttgcccttgg gtcgttttgc
    18061 gagttgaagt caagaagagg aaaaaaacaa aaaggagaaa tactcatggc agtaatttca
    18121 atgaaacaac ttcttgaggc tggtgtacac tttggtcacc aaactcgtcg ctggaatcct
    18181 aagatggcta agtacatctt tactgaacgt aacggaatcc acgttatcga cttgcaacaa
    18241 actgtaaaat acgctgacca agcatacgac ttcatgcgtg atgcagcagc taacgatgca
    18301 gttgtattgt tcgttggtac taagaaacaa gcagctgatg cagttgctga agaagcagta
    18361 cgttcaggtc aatacttcat caaccaccgt tggttgggtg gaactcttac aaactgggga
    18421 acaatccaaa aacgtatcgc tcgtttgaaa gaaattaaac gtatggaaga agatggaact
    18481 ttcgaagttc ttcctaagaa agaagttgca cttcttaaca aacaacgtgc gcgtcttgaa
    18541 aaattcttgg gcggtatcga agatatgcct cgtatcccag atgtgatgta cgtagttgac
    18601 ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat tgggaatccc agttgtagcg
    18661 atggttgaca ccaatactga tccagatgat atcgatgtaa tcatcccagc taacgatgac
    18721 gctatccgtg ctgttaaatt gatcacagct aaattggctg acgctattat cgaaggacgt
    18781 caaggtgagg atgcagtagc agttgaagca gaatttgcag cttcagaaac tcaagcagat
    18841 tcaattgaag aaatcgttga agttgtagaa ggtgacaacg cttaatttat acaaatagta
    18901 attacctagg agggcggggc ttagcccggc tctcctattt tcaaaaatat aggagaatta
    18961 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
    19021 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
    19081 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
    19141 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aatgctgaaa
    19201 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
    19261 ttgctgaagg aaaacctgct aacaatgaag aagctcttgc tttgataatg ccttcaggtg
    19321 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
    19381 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
    19441 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
    19501 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
    19561 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
    19621 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa gctcaattaa
    19681 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggca
    19741 aaccagaaaa aatctgggac aaaattatcc caggtaaaat ggatcgcttc atgcttgata
    19801 atactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
    19861 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
    19921 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
    19981 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
    20041 gggcttagat ttttcctgaa attgtttttt ccccagtctc actcgattag aataattaaa
    20101 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
    20161 aagcgcgtca taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa
    20221 ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt
    20281 tcaccttgtt tgtggcaaga cagatatgag gtagggaata gactcattag cctatgtact
    20341 taaaacccac tttgaattgg attctttctc cggtcaagtc tttctctttt gtggtggacg
    20401 taaagaccgc tttaaagtcc tttactggga tggtcaagga ttttggctac tatataaacg
    20461 ctttgagaac ggaaaactga cttggct
//
LOCUS       DAVBCR010000034        19957 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCR010000034 DAVBCR010000000
VERSION     DAVBCR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.034"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            380..1333
                     /locus_tag="VQ508_001154"
     CDS             380..1333
                     /locus_tag="VQ508_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2490159.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1384..2574
                     /locus_tag="VQ508_001155"
     CDS             1384..2574
                     /locus_tag="VQ508_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2490160.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ508_001156"
     CDS             2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ508_001156"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2490161.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3069..3893
                     /locus_tag="VQ508_001157"
     CDS             3069..3893
                     /locus_tag="VQ508_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2490162.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3912..4898
                     /locus_tag="VQ508_001158"
     CDS             3912..4898
                     /locus_tag="VQ508_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2490163.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4949..5572
                     /locus_tag="VQ508_001159"
     CDS             4949..5572
                     /locus_tag="VQ508_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2490164.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5703..7160
                     /locus_tag="VQ508_001160"
     CDS             5703..7160
                     /locus_tag="VQ508_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2490165.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7183..7464
                     /locus_tag="VQ508_001161"
     CDS             7183..7464
                     /locus_tag="VQ508_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2490166.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7541..8026
                     /locus_tag="VQ508_001162"
     CDS             7541..8026
                     /locus_tag="VQ508_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2490167.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8043..8708
                     /locus_tag="VQ508_001163"
     CDS             8043..8708
                     /locus_tag="VQ508_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2490168.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8712..9575
                     /locus_tag="VQ508_001164"
     CDS             8712..9575
                     /locus_tag="VQ508_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2490169.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9577..10281
                     /locus_tag="VQ508_001165"
     CDS             9577..10281
                     /locus_tag="VQ508_001165"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2490170.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10460..12133
                     /locus_tag="VQ508_001166"
     CDS             10460..12133
                     /locus_tag="VQ508_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2490171.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ508_001167"
     CDS             12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ508_001167"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2490172.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13450..15426
                     /gene="tkt"
                     /locus_tag="VQ508_001168"
     CDS             13450..15426
                     /gene="tkt"
                     /locus_tag="VQ508_001168"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2490173.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15543..15842
                     /gene="yajC"
                     /locus_tag="VQ508_001169"
     CDS             15543..15842
                     /gene="yajC"
                     /locus_tag="VQ508_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2490174.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15889..16317
                     /locus_tag="VQ508_001170"
     CDS             15889..16317
                     /locus_tag="VQ508_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2490175.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16319..16729
                     /locus_tag="VQ508_001171"
     CDS             16319..16729
                     /locus_tag="VQ508_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490176.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17029..19680
                     /gene="adhE"
                     /locus_tag="VQ508_001172"
                     /gene_synonym="adhC"
     CDS             17029..19680
                     /gene="adhE"
                     /locus_tag="VQ508_001172"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2490177.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6259 a         3575 c         4326 g         5797 t
ORIGIN      
        1 gtgcattggt tagttgttta ctttcttcat aaattttatt gtatcatatt tttgtttcac
       61 aggaagtcta ataaataaaa attctttaaa attttgttgg gaaaatggga aaagatataa
      121 atggaaactt agtataacac ttggaaaact ctgtaaaaat agtaagtcaa ttcatgtccc
      181 gttttttctc tagggaagtt gggcggctat atttatatga ccatttgtcg taataaacat
      241 ccctaccaag ggtcaataga aataaattca acgaaacttt tgctgtcaga tttagatgcc
      301 caatttgtca tttatactct ttcatatagg actttctata aggatggaaa aaagaaggga
      361 aatttgataa gataggaata tggattttga aaaaattgaa caagcttata cctatttact
      421 agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg ccttggtgga
      481 gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag acaacaatca
      541 ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc agtttctctt
      601 gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac cggatgctat
      661 tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa ttactatcct
      721 cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgatctcgc ttgataaaaa
      781 ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag ctagcatggc
      841 agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc cacaaatgct
      901 caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg atgatgccgt
      961 tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc acttgctcat
     1021 ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag ctccgagtga
     1081 tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag aggcgagtct
     1141 ggttgctatg attagtctgc ctgaaaatct ctttgctaat gctaaacaat ctaagactat
     1201 ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc cacttgctag
     1261 cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat ggactcaagg
     1321 tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa ggggtatcat
     1381 gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg gcaattatac
     1441 ttaatgccag aggaaaaagt gttagccaaa ggtttgattg aacgtatcgg tttgaaagat
     1501 tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga tatcgaaaat
     1561 catacacaag ctgttaaaat tttattggat gacttgattc gtttcgatat tatcaaggct
     1621 tatgacgaga ttacaggtgt tggccaccgt gttgtagcag gtggagaata tttcaaagaa
     1681 tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt gttggctcct
     1741 ctacacaacc cggccaatgc agcaggtgtt cgtgctttca aggaattgtt gccagacatt
     1801 accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa agcttatcgc
     1861 taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg tgctcatggt
     1921 acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc attagaagac
     1981 ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt gaaagccggc
     2041 aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat gggaacgcgt
     2101 acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga ggattttaac
     2161 acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg agtttctgct
     2221 aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca cgaggctagc
     2281 ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta ccttgcagtg
     2341 ctaaatggaa cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc agagaatttc
     2401 cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga tgaaaagaat
     2461 gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt cttggttatt
     2521 ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa ataagtgaaa
     2581 ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc tttttctgat
     2641 gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat aatagtagta
     2701 atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag agaaagattt
     2761 taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg tctaccaatt
     2821 agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac tggggaatgc
     2881 cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg caaaagggag
     2941 tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa ccttgggata
     3001 cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt accgggaagg
     3061 aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct ctgttaatca
     3121 tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg gctgattttt
     3181 ggagtaaatt ggtttacttc tttgcgaaaa tcattcgctt tttatcgttt gatattagta
     3241 tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg ccagtctttc
     3301 aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt aaggcgcttc
     3361 gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag gaaatgcgta
     3421 aagtatttaa agaaatgggt gtcagacagt cagattctct ttggccgatt ttgattcaga
     3481 tgccggttat tttggccttg ttccaagccc tatcaagagt tgacttttta aagacaggtc
     3541 atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg attttagcag
     3601 cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag cgaaatggcg
     3661 ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca gtttatgcgc
     3721 caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg caaacctatt
     3781 tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag gcacaaaaag
     3841 atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa taaataagga
     3901 ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc cagaaaggat
     3961 tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct agggagaaaa
     4021 aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa gcgattagtg
     4081 aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa aaaatcaatg
     4141 atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt catgtggttg
     4201 atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc aaggctgaaa
     4261 tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac attgagattt
     4321 taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat gaccttgaaa
     4381 ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag gtcgagcaaa
     4441 gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg attgtggatg
     4501 acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc aatctacaaa
     4561 ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg aaggccttgc
     4621 aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac gttacaatca
     4681 atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg caaaaattgg
     4741 cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg aatagtgaac
     4801 gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac tctgaaggtg
     4861 atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg ttgacctgat
     4921 tttttgctag ctagaggagg ttaaactaat gttgaataag ataagagact atttagactt
     4981 tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga agatgctggc
     5041 attccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
     5101 agcaagccat ccaggatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
     5161 tttgagacca catttttggg tttatctaca gagagacgaa caagtgacag aacctatgat
     5221 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
     5281 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
     5341 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
     5401 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
     5461 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
     5521 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
     5581 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
     5641 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
     5701 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
     5761 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
     5821 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
     5881 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     5941 gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa attgcagcag
     6001 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     6061 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     6121 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     6181 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     6241 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     6301 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     6361 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     6421 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     6481 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     6541 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     6601 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     6661 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     6721 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     6781 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     6841 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     6901 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     6961 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     7021 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttcttgc
     7081 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     7141 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     7201 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     7261 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     7321 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     7381 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     7441 aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac tgagagttat
     7501 cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa aacaagcttt
     7561 aattgacaat gactcgatcc gactaggttt agaggctaac aattggaaag aagcagtcaa
     7621 ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt acgatgctat
     7681 cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta tggctatgcc
     7741 ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta ccttacaaaa
     7801 tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag cagcaacaag
     7861 ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg aattagaaga
     7921 ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta tgattgaaga
     7981 atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag aggaataaag
     8041 aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac ttgcaaggag
     8101 cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct ggaactgttt
     8161 gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc ccagataaga
     8221 ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa aataatgcgg
     8281 ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact atggaagcag
     8341 ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc gagctttatg
     8401 gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca caagctattt
     8461 atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa gaccttaata
     8521 aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt ctagatgtag
     8581 atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt cgtggaatta
     8641 cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc aaacgaattt
     8701 gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca acacacttta
     8761 cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc gagatgtcta
     8821 ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa cgcttggaag
     8881 ttgtcaaaga aatctatgaa actggtgttc gtattccttc tatctgtttt tcaggccatc
     8941 gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta gaactcatga
     9001 aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta gctggttacg
     9061 atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa aatttgagaa
     9121 aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt atggatgatc
     9181 ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa agagattgac tctcccttcc
     9241 tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc tatagtgagt
     9301 tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat gcagtgacag
     9361 aaagttcaag gggccagttc cgagatgtac cttttgggca aggttgtgtc aaatgggaag
     9421 aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc gaaatgtggt
     9481 ctgaaaattg tgaaacagta gaagaaacac gcgcagccat tcaagaggcg caagcttttc
     9541 tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat caatgctatg
     9601 cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt caaatttacc
     9661 tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca ttgttatcaa accatcaggc
     9721 gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga tggtaagatc
     9781 ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt atataaggct
     9841 tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgc ttgggctcag
     9901 gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta cggttcaatc
     9961 ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa agatactggc
    10021 ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt accaggaatt
    10081 gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc tgtttatcac
    10141 tctgtcgtac tagaggaagt atcaaagatg aatcgcttta cagaacaaat caatccaaga
    10201 gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca tggaccaaat
    10261 gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat ttagctcctc
    10321 tttttacatt tgatttttat tgagagtaaa gttggagttg aagtaatttt aaaagatttt
    10381 ttagaaatag cgcttgatat atatatggta aaataaaaag aattgctgtg atatcaatag
    10441 atttggggga ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt
    10501 gatggatcaa gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag
    10561 aaggaaaatt tattatcaca ttgacaaaat caatgctgct ctgggtgacg aggcgcttca
    10621 catcattagt attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg
    10681 taaactatta tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat
    10741 gataatgtta ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga
    10801 gttaacagag gtatctagga atactgttct caatgatttg aatagtattc gttatcaact
    10861 aactttggaa caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca
    10921 tgcccaccct cttaataaaa ttcagtatct tcaatcgctt ctatatcata tttttatgga
    10981 agaaaatgcc acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga
    11041 gtgtttgctt tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca
    11101 agatttaggg aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta
    11161 tttgctgtta agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa
    11221 agaattttct ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg
    11281 gttgtttcaa aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct
    11341 cctcctctcc tatcgtaaag atttggatat tcatgcagaa agtgatgatt ttcggcaatt
    11401 aaaacttgct ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga
    11461 gaacaaggat gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa
    11521 gacttacggt attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga
    11581 attattttta gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct
    11641 aacagacgat gatattgcct atttgacgat tcatattgga ggatttttaa aatatacacc
    11701 atcatctcaa aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc
    11761 gagacttttg ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt
    11821 tacaacagaa caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac
    11881 taatgatgat ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga
    11941 agatattttg aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag
    12001 tttcagtgaa aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag
    12061 taagttccaa gaagaggttc aaacacttat aaatcaagaa atagtagttc aagctttttt
    12121 ggaagatatt tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt
    12181 ttagtgtttt gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca
    12241 acatatgcca aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga
    12301 gtggggaaca tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc
    12361 catgtggtgg ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt
    12421 catggacctt tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg
    12481 gcaccaaatg gcaaatatgg caggtgttcg taagctgcaa ccaaacttgc gtgttcagcc
    12541 aatggttatc gatccatttg ctatcaacga actagactat tacttagttt cacacttcca
    12601 cagtgatcat atcgacccat acacagctgc agcaattctc aataatccta agttagagca
    12661 tgttaagttt atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa
    12721 agaacgtatc atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca
    12781 tgcagtagaa tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga
    12841 gacaggcggt gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt
    12901 taactatatc tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc
    12961 aaactatttt gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg
    13021 tgaaaatccg gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga
    13081 aaatctgcgt accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc
    13141 ttctactaat gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt
    13201 ccatccattt atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt
    13261 agaataccat catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt
    13321 taaagctttg ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg
    13381 gataattttc atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa
    13441 ggaaattcta tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt
    13501 aataaagcca actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc
    13561 ctctttacaa aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc
    13621 tttattcttt cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt
    13681 tttgaagatg tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca
    13741 ggtcacccag aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa
    13801 gggatttcaa ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac
    13861 cgtgaaggtt acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg
    13921 atggaaggtg tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg
    13981 gttgttcttt atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca
    14041 gaaagtgttc gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga
    14101 acagacttgg aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct
    14161 ttgattgaag tgaagacggt tattggatac ggttctccaa acaaacaagg aactaatgct
    14221 gtacacggcg cccctcttgg agcagatgaa actgcatcaa ctcgtcaagc cctcggttgg
    14281 gactacgaac catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca
    14341 gaccgtggcg catcagctta tcaagcttgg actaaattag ttacagatta taaagaagct
    14401 catccagaac tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg
    14461 actccagcag acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt
    14521 caagatgcct tgaatgttgt ggcagctaaa ttaccaacct ttctaggtgg atcagctgac
    14581 cttgctcact caaacatgac ttatatcaag acggatggac ttcaagacga tgccaatcgc
    14641 ttgaatcgta acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg
    14701 atggcccttc atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat
    14761 gtgaaggcag ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc
    14821 catgattcaa tcgcagttgg ggaagatggt ccgactcatg aaccagttga gcatttagca
    14881 ggtcttcgtg ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa
    14941 gcagcttggt accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt
    15001 caaaatttga ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt
    15061 gtatatgaaa atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat
    15121 cttgctgtct cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc
    15181 atgccatcta cagatgtctt tgataaacaa gatgcagctt acaaggaaga aattcttcca
    15241 aatgcagtcc gccgtcgtgt tgcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat
    15301 gttggtctcg atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca
    15361 aaagtattgg cagaatatgg ctttactgta gaaaatcttg taaaagttgt tcgaaacttg
    15421 aaataatcct aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca
    15481 atcttgtaaa agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa
    15541 caatgaatcc aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct
    15601 ttatgcaacg ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac
    15661 aaaaaggcta tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata
    15721 cggagaaggg aacaatagtt cttgacgtag agggagttta cttgactttt gaactagctg
    15781 ctatcaagac agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat
    15841 aagacgggat cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta
    15901 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    15961 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    16021 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    16081 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    16141 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    16201 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    16261 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    16321 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    16381 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    16441 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    16501 cggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    16561 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    16621 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    16681 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    16741 caaaggaggt attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca
    16801 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    16861 caaagataaa acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    16921 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    16981 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    17041 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    17101 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    17161 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    17221 tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt
    17281 gaacacgtgg taaacaacat gcgtcacact aaaacagttg gcgttatcga agaagatgat
    17341 gtaacaggat tgaccctgat tgctgaacca gttggtgttg tttgtggtat cactccaaca
    17401 acaaacccaa catcaacagc gatcttcaaa tcattgattt cattgaagac acgtaatcca
    17461 atcgtctttg ccttccatcc atcagctcaa gaatcatcag ctcatgcagc tcgtatcgtc
    17521 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tacagtggat cactgaacca
    17581 tctatggaag caactagtgc ccttatgaac cacgaaggta ttgcaacaat ccttgcaaca
    17641 ggtggtaatg ccatggttaa agccgcatac tcatgtggta aaccagctct tggggtaggt
    17701 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    17761 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    17821 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    17881 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    17941 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    18001 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    18061 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    18121 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    18181 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    18241 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    18301 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    18361 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    18421 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    18481 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    18541 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    18601 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    18661 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    18721 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    18781 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    18841 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    18901 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    18961 actgtggcaa tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat
    19021 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    19081 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    19141 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    19201 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    19261 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    19321 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    19381 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    19441 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    19501 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc
    19561 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    19621 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    19681 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    19741 tctatgataa aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga
    19801 tattgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt
    19861 gaaactggaa tagtacactt ctgcttctaa aacattgtta gaattcgatt tgactgtcct
    19921 gatcgatttg tcctattcct atttcatttt actatat
//
LOCUS       DAVBCR010000035        19827 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCR010000035 DAVBCR010000000
VERSION     DAVBCR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.035"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..611)
                     /locus_tag="VQ508_001173"
     CDS             complement(<1..611)
                     /locus_tag="VQ508_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2490178.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQL"
     gene            complement(720..1238)
                     /locus_tag="VQ508_001174"
     CDS             complement(720..1238)
                     /locus_tag="VQ508_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2490179.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1465..2334)
                     /gene="rsmI"
                     /locus_tag="VQ508_001175"
     CDS             complement(1465..2334)
                     /gene="rsmI"
                     /locus_tag="VQ508_001175"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2490180.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2337..2654)
                     /gene="yabA"
                     /locus_tag="VQ508_001176"
     CDS             complement(2337..2654)
                     /gene="yabA"
                     /locus_tag="VQ508_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2490181.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2694..3584)
                     /locus_tag="VQ508_001177"
     CDS             complement(2694..3584)
                     /locus_tag="VQ508_001177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2490182.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3581..4219)
                     /locus_tag="VQ508_001178"
     CDS             complement(3581..4219)
                     /locus_tag="VQ508_001178"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2490183.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4439..5236)
                     /gene="proC"
                     /locus_tag="VQ508_001179"
     CDS             complement(4439..5236)
                     /gene="proC"
                     /locus_tag="VQ508_001179"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2490184.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5240..6502)
                     /locus_tag="VQ508_001180"
     CDS             complement(5240..6502)
                     /locus_tag="VQ508_001180"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2490185.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6512..7642)
                     /locus_tag="VQ508_001181"
     CDS             complement(6512..7642)
                     /locus_tag="VQ508_001181"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2490186.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7725..9605)
                     /gene="cbpE"
                     /locus_tag="VQ508_001182"
                     /gene_synonym="pce"
     CDS             complement(7725..9605)
                     /gene="cbpE"
                     /locus_tag="VQ508_001182"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2490187.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(9608..10495)
                     /locus_tag="VQ508_001183"
     CDS             complement(9608..10495)
                     /locus_tag="VQ508_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2490188.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10485..10946)
                     /locus_tag="VQ508_001184"
     CDS             complement(10485..10946)
                     /locus_tag="VQ508_001184"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2490189.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10943..11851)
                     /locus_tag="VQ508_001185"
     CDS             complement(10943..11851)
                     /locus_tag="VQ508_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2490190.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11844..12041
                     /locus_tag="VQ508_001186"
     CDS             11844..12041
                     /locus_tag="VQ508_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490191.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12093..12764)
                     /locus_tag="VQ508_001187"
     CDS             complement(12093..12764)
                     /locus_tag="VQ508_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490192.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12818..13039)
                     /locus_tag="VQ508_001188"
     CDS             complement(12818..13039)
                     /locus_tag="VQ508_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2490193.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13311..14120
                     /locus_tag="VQ508_001189"
     CDS             13311..14120
                     /locus_tag="VQ508_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2490194.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14301..15176)
                     /gene="aguB"
                     /locus_tag="VQ508_001190"
     CDS             complement(14301..15176)
                     /gene="aguB"
                     /locus_tag="VQ508_001190"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2490195.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15186..16271)
                     /gene="aguA"
                     /locus_tag="VQ508_001191"
     CDS             complement(15186..16271)
                     /gene="aguA"
                     /locus_tag="VQ508_001191"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2490196.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16268..17395)
                     /gene="nspC"
                     /locus_tag="VQ508_001192"
     CDS             complement(16268..17395)
                     /gene="nspC"
                     /locus_tag="VQ508_001192"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2490197.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17395..18654)
                     /locus_tag="VQ508_001193"
     CDS             complement(17395..18654)
                     /locus_tag="VQ508_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2490198.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(18651..19511)
                     /gene="speE"
                     /locus_tag="VQ508_001194"
     CDS             complement(18651..19511)
                     /gene="speE"
                     /locus_tag="VQ508_001194"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2490199.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5743 a         4558 c         3537 g         5989 t
ORIGIN      
        1 tcaagctgtg agttcattat gatttgtaaa tccgatatat cttcagcagc taaaaacaca
       61 ttttgaaatg ttccaagtcc gttttcacca aaactatatt ctctcttact atacttactc
      121 ttattattct ctatatagtt agagtcaagg ttcttaacct cttgaagttc attttcttta
      181 cttctgaggt taaaatcttt tacttctgaa gttaagtttt ttgacttctg taagtcattt
      241 tcttttacct gaaatatact cataaagtct ttaacataaa tgatgttcgg cttaccaagt
      301 ccaagcctta ctctttcgat cagtcctatt ccttttttta catcaagctc atctaatgtt
      361 tttatggcag ttggctttga gatatttctt cttttcataa tttcttcgac agtaaaatag
      421 ataaatactc tgccttcctt gtctatccag ttattcttaa atgacattcc tgtctgttta
      481 agaagtatgg aataaaggat aattgcttct gctgataagc cccgaaattc ttctctatca
      541 actaatatct ccggtacttt taagaagtta aatctttccg cttctctgtt ataaaaatat
      601 tcaaagtcca tgcaagatca cctcccttct taaaaatttg caaagaaaaa gacgatagtt
      661 tttcttctat cgcctttggt aaccattttt atgaaatttt ttaaagttaa aaatataatt
      721 tataatacta tcttcttcca ttttctgatt ttagttctga aagttccaaa aggtgcaacc
      781 gtatttacat gaataaactt atacacttcc caagtcgctg ttttagtcgc ttcatcagcc
      841 cactttctca tatgcggttc aaataattct tcctcactca acgaatcaat cattgcagag
      901 atagaattaa tattttcatt taattttgct ttcaactctt gcagagataa atgagcgtag
      961 gtatctgtga accactgata taattcacca agttgattcc atttaaattt atccgatggt
     1021 gtttttactt gaagcccctt tctttcatct tcttcccatt taagaaccaa gttggtccaa
     1081 ccaacctgat aagcaaggtt ttctgctgga gttctgtcaa cttcatcagc tctcttatct
     1141 tttaaatttt ctggaatatt atcaaattct aaaatatatt tctcaaatgt tttctctatc
     1201 tcagctttta gttcttcttt attttcgtat gttcgcaaaa aatcaccttc tttctgattt
     1261 atcaatcctt tgatttaaaa tgaccttatt tttctattta taccttctaa gaacgctata
     1321 actgctgctc caaacatcgg tattccgtat gggcttaata aaaaacttaa taccaaagct
     1381 tctataccaa gtgatacatc tttatattat catcactaat taagttaaac agaaacagac
     1441 agaattatta catcctgtct ccctttattg tttttcttcc caatcgtggt aggcagcgta
     1501 gagttgactc ttattccact ggtaaatctt agctacttcc ttaatagctt gatttttctt
     1561 catgccttgc tggatacggg cttggatttc taagaacaag tcctcctcgt ctttttcctc
     1621 cacatcctga ctggcacctt caacaataag aaggcattcg cccttgagtg gcgtttcagc
     1681 gatgctttcc agtaattcag aaattgtacc tcgttggtat tcttcataga ttttggtcaa
     1741 ttccctgacc aaaacaaccg agcggtcacc gtagacttct aacatatttt ccaacgtgtc
     1801 tgctacacga tgaggtgatt cataaaaaat ctgtgtttca ggataatctt ttttagagcc
     1861 aaaaaattgc ttctgttgac ctgattttct cggtaaaaaa ccgtaaaaga tatgtggctg
     1921 tggcgctaaa ccactggcaa tcaaggcaga aattcctgca gaggtacctg gaacagtcac
     1981 aactgcaatt tcttcctcaa tagctgcctt aactaaatca tgaccagggt ctgaaatgct
     2041 aggcaaaccg gcatcagaga cctgagcaat actttgccct gctttcaaga aaccaatcaa
     2101 atcaggaatt ttttccttgg cattgtgctc atgaaaactg atctgcttgg tggaaatgtc
     2161 aaaatgcttg agcaaaagcc ctgtattgcg cgtatcctca gcagcaatcc agtccacttc
     2221 tttcaaggtc tggatagcac gaaaagtcat atcatctaga ttgccaatcg gcgttgccac
     2281 tagatacagc ttgccatagg gagactgccc cttaaaactt ttttgaattt gcatgcttac
     2341 tccctgtata acaactcatc acagaacata cattcttcgt cctgctctcg acgttgtcca
     2401 taaaaatcat tacatacgtg aaatccatca cggtaaatac gacggacact ttcgcgaaca
     2461 tgcttggcct tgacgggagc gtctgcttcc acctcaccca agcgttctcg caacttacta
     2521 ttttccaagc gaagagctgt attttcttct accaagctct tgagattttt cttgatggct
     2581 tccacatcgg ctaaagttac caataattgt tgggaaaaat catccagcgc gtcaaataat
     2641 tcttttttgt ccataaaaca gccctttcct ttctttatca ttcatttttg agtttatatt
     2701 tctttcaaga ccagatattc catggcattt tgaaagctga cattagcttg ccacattttt
     2761 ctagcttcta gtaaatcttg tagaatcact cttactctta cctgcaagag gtcctgccca
     2821 cagagaactt caagaatccg taaaacctga tcctgttttt ccttatcatc tgccaagttg
     2881 gctaatttgg caacctgtag ataactttct tttttcttag ctactaacca agtcagcagg
     2941 cgttcacttt catcgaccaa ggtccaaaaa cttgcctgat tagccaactt ttctgcttca
     3001 gctcgcgatt gactaaactt agctaaaaga gtcgcttttt tcttaacaag tcccatttgt
     3061 tctaagagta agataagttt ttcttcttgc tttttaaagt ggaagatctg agtccgactt
     3121 cggattgtcg gtaacatctt ttcctcatcg ctagtcaaga agaaaatata aacttcactc
     3181 tggggttctt cgatgacctt gagcagagaa ttggctgcgt tgggatgcat tttatccgct
     3241 tgctcgatga taaagacctg ttgctggctt tcaatccctg cttgagaaaa ctgtcccacc
     3301 aattcccgaa tacgttctgt cttgatgacc tgattgactg gcttaatcaa ggtgacatct
     3361 ggaaactctt cctgttcaat cagcttgcaa cttcggcatt tctcacatgg taagacgcca
     3421 actttatccg tacaaaagag gctcttagct aaaaattgcg ccatttccaa gcttccaaag
     3481 aaacctgaaa agagataggc gtgattgagc tggtcttgtt ctaagatacg gacaaaacgg
     3541 tcaaactgag ctggttgcca agcctttagt tgatcttgtt tcatttggcc aagcccattc
     3601 cgtcaaacaa gacagccttg gtagtttcca caacttgctc caaagggaga ctagcatcaa
     3661 tcttgacaat gcgatttccc tctttatcca gaagagaaag gtagccttga cgaacttttt
     3721 tatgcaagtc caacccttcc aaatccaaac gattaacctc gcggtcacta ttagcagcaa
     3781 tacgagccag cccttcttcc acctcgatgt caaaatagag tgtcaaatcg ggtttgaggc
     3841 catctgtcgc aaactgattg agccagtcaa tggcttcaat atctaagcca cgaccaaatc
     3901 cctgataggc aacagaacta tcgataaaac gatccatgat gaccaacttg ccagcttcaa
     3961 gggctggaag aactttttcc accaaatgct gtctgcgact ggcaatatag agaagtagct
     4021 ctgttttagc atccatctga gtatgacttg gatccaaaat cacttcccga atcttctccc
     4081 caatcaagac tccgccaggt tcacgggtcg tcagcacctt tactcctttt tcctctaaaa
     4141 ttggtagcag agcctctaaa acactggtct tgcctgctcc ctctggtccc tcaagagaga
     4201 ctaaaaatcc ttttgacatg tctaactcat ttctttttta ctacttctat tctatcaaaa
     4261 aaataggggt ttgtgacaat ctttttgtct tctcatttca tactcaatga aaatcaaaga
     4321 gcaaactagg aagctagccg caggctgtac ttgagtacgg caaggtgaag ctgacgcagt
     4381 ttgaatttga ttttcgaaga gtatcagcca ccataaaaga ggcagataaa actacctctt
     4441 atttacctag ttcttgtgtt cgtttatagg cttgatgaac tgcctccatg actgtgcctc
     4501 gaaaagcatg cgcttctagg cttgctacgc cagcaatagt cgaaccgcct ggactacaaa
     4561 cttgatcttt caaaactcca ggatgttgct ggctttctag gaccaattgc ccagctccta
     4621 ccacagtttg ggctgccatt ttcaaggctg tttctcgtgg taatcccgtc tgaacacctg
     4681 catctgccaa ggcctcaata aagagataga caaaggctgg tccacaacct gcaagacctg
     4741 tcgctgcatt gattaagctt tctcctaatt caaccaagag accagccttg gctaaaagct
     4801 gacaaaagat ctcactgtcc tcagccctgc aattaggaga caaggcataa ctaatcactc
     4861 cttgcccgat agaagcaggg gtattaggca tcatacgaat aattcggtgt tggcttggga
     4921 taagacttgc tagtttttct aaggtcaatc cagctgccat cgaaatcaaa agaagacttt
     4981 ctcttttttc aaggatggtc tggtattgag aaagcagttc agaaaactga gcaggcttaa
     5041 cacctagaaa aatcacatct gcttcttcga atatttcttc attgttagaa gtctgaccac
     5101 cgaagttagc gatgaaagca tccaccttga cttgactacg attggcaagg agaatctcat
     5161 ctgacggtct agcctgcaag acagacttgg ttaaactagc acccatattc cccaaaccga
     5221 taaatccaat cttcatctct tactccctta tctgtccatc accagtgacc acatacttgt
     5281 agctagtcaa ttccttcaag cccataggac cacgcgcgtg caatttctga gtagaaatcc
     5341 ccatttcaca accaagacca aattgtcctc catctgtgaa acgagttgag gcattaacat
     5401 acaccgctgc agagtccact tgatctgtaa agtatgctgc agcttcagca ttttccgtca
     5461 caatggcatc cgaatgatgg gtactgtggg attcaatatg cgcaaccgct tcttctaaac
     5521 tgctcacaac cttaacagct agaatatagt ctaaaaactc ggtatcaaag tcttgtgctt
     5581 gagcagcttg acctgaaaca aactggcttg ctttgctatc taggcggaat tgaattggtt
     5641 ccaacccagc ttcttttcga tctgcaacca acacttgctc caagcgagga aggaagctta
     5701 ctgctttgtt ttcatgaacc agcagaacct ccatggcatt acaaacagaa ggacgactgg
     5761 ttttagcatt gttgatgata gacagcgcct tgtcttcgtc tgcatcctta tccacataga
     5821 catggacaat cccagttcct gtctcgataa caggtacaat cgcattctca accactgcat
     5881 tgatcaagcc agctcctcca cgaggaatga gaaggtctag atagcccttg gccttcatca
     5941 tagcataact actttcacgg ctagtatcct ccaccagttg aatcacattt ggatgaatag
     6001 tagtcgtctc caagcccttc ttcaaggctg tgacaatggc atgggttgtt tgataggcat
     6061 ccttaccact acgaagaaca accgcatttc cactcttaag agtcaaagca gccgcatcag
     6121 acgtcacatt tggacggctt tcatagataa taccgatgac acccatagct acacgttttt
     6181 ttgtgataac caaaccattt tcaagctgac ttgtttctaa aacttcaccg attggatctg
     6241 gtaaggcaac cacttcacga attcctcttg ccatcgcttc tatacgatct gcatccaaat
     6301 aaagacgatc caacatcaca tctgagattt tccccttagc cgctgccata tcgagggcat
     6361 tagccgctaa aatttcctca gtagcagcca ctaagtgatc agccatggct agcaaggctt
     6421 ggtttttcac ttcttcacta gctgtgttaa tcgatttttt aacagcctgt acctgttcaa
     6481 attgttcttg tctactcacc atagtttacc tctaaaattc tgtaaaaagt agttggattt
     6541 caggagtaat ggaaatccag tcgtcacggt aaatcaagac acccttggct ttttgaaaac
     6601 gcaacatatc ctccaaagca gatgctccaa attgcacgcg tccttttcca agtgattttc
     6661 cactttcctt atcaaatact gtcacgatat caccgtaaga aaagactcct tctgcttcaa
     6721 cgataccaga taagagaaga ctctttccat gttgagagag agcttccgca gcccctttat
     6781 caacccaaat agaaccttga ctctgagcat agaaggcaag ccattgtttc tgggtacgaa
     6841 gccccttctc ttgagcaaca aagtaagaac catcctcggt ctcctctgcc gcctcaacca
     6901 tggaatctga tttcaaggat gagcagatat aaacaggaac tcctgattcc gtcgcgatag
     6961 ttgcagcctt gattttggtt aacatacccc cagttccgtt tgacgaacca gctccaccag
     7021 ccatatcaat aatctcacga ttgatggtct cgattctctc caagcgtttg gctcttggat
     7081 ctgaattagg atttccagta tagagaccgt ccacatctgt caagaaaact aaaaggtctg
     7141 cttggaccat cgccgctact tgagcactta gagtgtcatt gtccccaacc ttgagctcat
     7201 caataacgac actatcattc tcattgatga taggaattgc cccacggttg agcaaaaccg
     7261 acaaagcctg atgggcattt ttataacgac gcttatccac aaagtcatct tgggtcagca
     7321 agatttgtgc agaaacgatt tgacgcaaga gaagattggt cgtatattct tccaacaaaa
     7381 gtccctgccc taccgctgct gaagcctgtt tatcagcaat tttagtcgga cgctttttaa
     7441 atcctaaggc tccaaaacca gccgcaatgg cacctgaaga caccaaaatc aactcatgac
     7501 cagcctcgtg cagcatagcc aactgctggg taatatcctt taccttacta cgtgataaac
     7561 ttccatcctc atttgtcaga gaagaagtgc ccaccttaaa gacaatccgt ttgtatttca
     7621 tagtctcact ccgattattc atatttatcc attataacat gaataacagg aaatagaaaa
     7681 gagttgatag gaaaaagaag gccctaaaac cttcttttct actactattc tgattccgat
     7741 ttgtttacac tcgtttcaga gctttgtgaa gttgaggatg tcaaatcttg actagttgaa
     7801 gaatttgtaa cactttcatg ttgactcgtt tctttatttt caagatcctt tttcactact
     7861 tctttggatt cttttatttc tgaatgactt gtagttttaa ttgtagcaga tttctcgatc
     7921 ggaacatcca ctaaccaggc accacttgaa tccacagtat agccttctgg tgtcttacca
     7981 ttcacaagta attgaccatt ttctttcaag aagtaccatt tatccttgtc tttaatccaa
     8041 cctatagctc gtgaaccacc tgtcttgtag aaataccaat catttgcctt tttaagccag
     8101 ccttgcttca tagctcctga ttcttctaga tagtaatgat gtccagcaac ttctatctca
     8161 cctgtcttca tgataccagt agaatccata tagtaccagg cttctttatc ttttacccag
     8221 cccgttttca tttcccctga tggagcaaag taataccatt gccccttata ttgaagccaa
     8281 cctgtcttca tagagccatc aacatccaaa taataccact gctctttatc ttgaacccaa
     8341 ccaatttcca tctgattttc tttgttaaaa taataccaga ttccatcaat tttcttccaa
     8401 tttttagcag aagcaccaga gtctgtcaaa tagaaccaat gattgttcca ttttttccat
     8461 tgattctgta acaagatacc cgtttggtta aagtaatacc attcaccttc gatttcattc
     8521 caaccgacag catactctcc tgtagaatca ggcgcttgat accaccagtt cccatatgca
     8581 ctcttatgcc aaccagcttg aaaacttgga atcggcttgt aggatgttga aatattgaca
     8641 aaaccgtctt ttcgaatatc aaaaactgtt gcatcatagt ctttgctggc tgcgttgatt
     8701 ctctcaattc ctcgttcttt gagccaatta acatactcac tatcaacacc atttttccaa
     8761 ggtagactat ccgaagtttg aacaatcaaa ctcggactca aatttttaat gaaatccttg
     8821 gtatttgatt tgttggtatc atggtgatga ttaaacttca tcaaatcaac ttttccaatg
     8881 agaggaccat acttgtcttc tgctccatga acattatcta aatcgccccc aaggtaaatt
     8941 ttcttgccat tgactttcac cacgctaatc aaggaattgg aattgtcatc ccaaattttc
     9001 tttaattcac ccgatgaatc agtttcattt tcataattat agagctgaat atccatgtcc
     9061 ccaaactgaa aatgagcatc cccttgtgtg atattttgaa taactgaaac acctttttct
     9121 gcagcagtct gtaaaacctt atcatagcca tacagattat cccatagacg ttcagaatta
     9181 gtaatacgac tatcactata tttcttaaga tagactcggt caactggata ggtagacagt
     9241 aattcatcaa catttccaat atgatcactg tgggtatggg tcaccaaaat aaaatcaagt
     9301 ttttggacac ccaattcctt caaacgacga aagacacggt ctgttagaac atgcttataa
     9361 gacgtttcaa ttccttctct ccatggataa cgagaatcac ttccatctgg gaaatcataa
     9421 tcttctcctg tatccaccat ggcaaaatgt ccattgcttt caagaataat cgcatcactg
     9481 ccaccttctt gaacattgat aaagtggatt ttatttcctg aactttcttg agcctgaaca
     9541 ttcccatacc atgacaaacc taaaaaagcg cctacaagtg ctaaactagt taatttcttt
     9601 ttcattctta ctttctcaat ctctccaagg tttccttaaa aatctctggg atatctgctt
     9661 taaattccaa ggtcttacct gttctcggat gagtaaaacc taaagtcttg gcatgaagaa
     9721 attgtccatg tcctttcaaa gtcttgcgag gaccatagac ctcatcacca gcgactggat
     9781 ggccgatata agccatgtgg acacggattt gatgagtgcg ccctgtctcc agttgcaact
     9841 ctactaagct ataatcgcca aagcgttcca agacgtgaaa acgcgtcact gcaggcttcc
     9901 ctttagcagt tacagcctgt ttcttacggt ctttttcact ccggccaatc ggcgcttcaa
     9961 ttacaccacg atcattaggc agatttccat gaacaatcgc ccaatatttg cggagagact
    10021 ttttatcttt gagttcttgg gcaagtacta gatgcgcatc atcgttttta gcaatcatga
    10081 gaagacctga cgtatcctta tcaatacggt gaacaatccc tggacgcaga accccattga
    10141 tacccgacaa gtccttaata tgatacatga gggcatttac tagggttcca ctggtatgac
    10201 cagcactcgg gtgcacaacc attccctgag gtttgttaac gacagccaca tcctcatctt
    10261 ggtagactat ttctagcgga agatcctcag ccacatactc taatacctct ggttctggca
    10321 catggtaagt gacgacatca ccctcttgga ctgtgtattt agctttcttg acttgaccat
    10381 tgaccaagac ctggcctgat ttaatttgtt cattcgcgag actacgtgat aattctgaca
    10441 aatctgacaa agccttatcc aaacgcagac caccagtttc aattttaatt tccatttatt
    10501 tcctctttta gcattgcaat caataaaata atcactccaa ccgtcagata gctatctgcc
    10561 acattgaaaa ttgcaaagtt gataaagtca aggtggaaca tatccacaac aaagccctga
    10621 ctgaccctgt caataaagtt tccaagacca cccgcgatta ttagagtcaa acccaagaac
    10681 atccagaatg agtcctccat gtgtttatgt aaataccaaa tggcacctat cacgacaacc
    10741 agagtaatga cagcgaataa cagctgctga tcttgtaaga tagaaaaggc tgcacctcga
    10801 ttttgcaggt aggtcaagct aacgaaattg ggaatccagg agcgcacttc acccagtgga
    10861 atctgctgga cgatatagga tttgaccaac tgatccagcc caattaaaag cagtacaatg
    10921 actgccacta ttgctctttt tttcatgatt tcctcttttg atcaaaatat tcttgcatga
    10981 cttctacgaa gagagtccca gcttgactaa gctccacttc ttcacgttta acatagacca
    11041 tgcggttatc taggttatcc ttgagacgaa taactgtaat gccattaaca ctgtcactat
    11101 ctaaaaatcc agaacctgtc gcataggcgt ccgtccgctc caaaatacca ttcaaggtgg
    11161 cacggtctgt cacattaaac atctgtgagc tagcgctggt atcgacaaag ttctctgaat
    11221 aataaaggta ctcgtctttc tcttgagtga aacgaaccgt tggtaaatcc gctaaatcct
    11281 ccatgactaa ttcctctttc tgggctaaag gatgaccctc acagagataa atatgggtat
    11341 ggaaaggaat caattcgatg acctccagac ctaacttttc aacccgttgc ataatcccct
    11401 ttttattttg attgttgagg tagataatcc caatctcact atgcccttgc gccacttcat
    11461 ctaatatttg aacagtagtt gattcaaaaa tacggaagtt cttatagtca ggatagcgct
    11521 ctgaaaaggc cgtaatagtt ggtggtaaga agtcatagtg ctggctagca acggaaaatt
    11581 catctttttc ttcttcagga ttggcatact gattttgaaa aatatcaaat cctttaacca
    11641 attcttgcgc tttttcataa aattccatcc cacgacgggt caagaaagtc cctgagctgg
    11701 tccgacggaa aatcttaaag cccaactctt tttccaaatc acgaacagaa atagacagac
    11761 tcggctgact aacatacatc ttttcagcag cttcacgaaa agtaccacta ttggcaatag
    11821 ccacaacata gcgtaattgt tgaatgttca tcttctaccc ccaacttctt tatcttttca
    11881 ttataccata ttttagaagt tttccaaaaa ggaaaaaaga acatcctatt cttcttaact
    11941 atcttcacta tctgcctttc tcacgccaat cttattttca aaatcaattc aaaaaaataa
    12001 gtggctacac accacttcag tatcggaaag aaaaacgttg actatttgtg aaaaaagaaa
    12061 atgccggaaa attccgacat ttttttagta agctaacttc ctgaaaatag gagtaaccaa
    12121 acatcccaga aggcagtgat attgatgaga atatgaacta gtattggata gtagagattt
    12181 ttagtcatgc gatacaatag agctaaaatc agacctccac tagcataaat gaaaaaagca
    12241 agaggagtta aagcaaaatt gatatgaata taaccgaaaa taatagcaga aagcaaaaca
    12301 tctccgtacc aaggtgagtt tttgaaaaag gttgtcataa gcacacctcg ataaatcaat
    12361 tcctcagcaa taggggcgat gaagcaaacg atgagcaaga aataagggaa ctcctgtctc
    12421 cccatcattt ctaccgtttc attcaaagaa atctgatttg aagacaggga tatgaaatac
    12481 gaaaagagga agtcagacat atatgaaatg atgtagccca gtaaaaggta gatgaagtac
    12541 ctcagctgcc acttgaaatg aaaaattttc tttcctgcaa aaaccagtag atagatgacc
    12601 gctaagaaaa gaactccgct ctccatcaaa agcagaatct gaaaaaattc acgacttgct
    12661 ggtagatagg gctttgcgag gtgattgaaa actctccaaa accaagttga tttgtaaaaa
    12721 attaaggaca atgctagtaa aatttgaata gcccgttttt tcatattaaa ttctctgctt
    12781 tctccactta tttcttcata ttactaataa attttactta aaatctcgca gcacttcctt
    12841 tgcaaagatg attgcctcct ccaatatatc ctaaatcata ggtgcctctg ggacaaaatc
    12901 catgaaccgg cagagttaat actggttcta tttcccaatc gtacctacca ttaggatttt
    12961 taccatatga atctgctcca ccttgtatca gctctaattc ttcatttgtt agatttatat
    13021 tttttgtcat taaaatcatg atatatttcc tccattattc ttcagattag ttgagtaatc
    13081 tgatactcat acctacttac aaaaaaacta ttatattaag ttggtttttt aatttgtcta
    13141 gttgcaacat tgacaaacat agtatagcat atctttgcga aatatcctct tcaaatcatg
    13201 aattgtcatc aaaacatctt aaactataaa atcaattagt ctcaagcttt ctatcaattt
    13261 cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct atgattaaac
    13321 tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc ccacaagctc
    13381 acattactgc tattcacaaa gccattgaaa aaggtgtcaa actggttctc tgtacgggtc
    13441 gccccctttt cggtgtcctc ccctactaca aaaaactggg actcgacctc cagaatgagt
    13501 atgtgattgt taacaacggt tgttcaactc accagactag cgactggggc ttggttgact
    13561 ggcaagaact tagtccagct gacatcgaat acctctatga ccttgctgaa aagagtgatg
    13621 ttcagttgac actttttgac gagtcacatt attttgttct cggtggaaag cccaatcaag
    13681 ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct cttgaggaag
    13741 cgactagtgg aaagttccgg atgttccaag gtatgttttt aggaacaaaa gaacaaacag
    13801 acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga gttcgttcgc
    13861 agcctgtcat ttatgaagca atgccacttg gaacgacaaa ggctactgct ctttcacgac
    13921 tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat gctaataacg
    13981 atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc agcgattatg
    14041 tcaaatctct tgcggatgcc gttacctcaa gcaacgaaga agacggcgtt gcgcgtgcta
    14101 ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg ctatttgctt
    14161 ttttgctaat attttaaaat acggactaat caatagagaa gaatagcgaa tcataaaaat
    14221 cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc tagcagaatg
    14281 aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg tcttctatct
    14341 cgaaacaagc cccagtttag gcgttcactt gctcccttgt ctaggttata agtagctaac
    14401 agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc atccgtcata
    14461 aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc actaggagta
    14521 acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc tgctgcgtgc
    14581 ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaattgg ctctgaaccg
    14641 atagctgtag gataaaagag caattcagca ccattcaatg caagacagcg cgctgtttca
    14701 gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt attccagacc
    14761 ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg gtcatctggt
    14821 atatgggtct ttcgataaac gcccagcact tccccatctg catcaatgac ggcaatagag
    14881 ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac ttgtagttcc
    14941 ttagcaagca acttaaaatg ctgaatggca gtattttccg ctacagattg ggcatactgg
    15001 tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc gggcaagaga
    15061 ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt ttggatattt
    15121 gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt tctcatcttt
    15181 ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc taagagaata
    15241 tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag gatatctaag
    15301 gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt ggcgatataa
    15361 aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gctcttcttc tccttcttca
    15421 tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg gataggcaat
    15481 ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag ttcgagatct
    15541 gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagccaa aacaacctca
    15601 gcaggaccaa caaaggcaac aacattatcg acgtgttcat tggtttcatc ctgataaata
    15661 ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt gttttcaatc
    15721 tcctctttag tcaagttagg attgcgacca ggactaagca agcaactttc agttacgaga
    15781 atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg tttagcatca
    15841 tagacaggcc tttccaaggc ctcagcaaaa cgactggcta cttggtcatc ctcttcataa
    15901 tcttgataaa gaccatcata ggtgcctccc caagcattaa aggcccaatc cacggctaat
    15961 ttcttacctt tatcattgac gagaatggtt gggccagtat cacgcgccca ggcatcattg
    16021 gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc agatagatag
    16081 gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat gatctgagta
    16141 aatgctcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt cggccatatc
    16201 atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa tttttttgga
    16261 ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg agtaagctac
    16321 agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta aaggtattat
    16381 ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg atttggactg
    16441 gattttcaaa actataatca ccaatcacat cgccagtcag acaggtatta gaagaaagtc
    16501 tgtaggtatg ggctttttcc tgtgactcaa agccatttct caaaggtgga cgatagggca
    16561 tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag atttccatac
    16621 cgttttctac aatatctaat acctcagttg ctaaataacc cgcattaagc gcaatggctt
    16681 caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg atttctgaaa
    16741 tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc atattgagcc
    16801 attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa gttgtttgta
    16861 aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc aaatctagca
    16921 aatcgctcgg aatcttgtct atagtaactc caaagcgaga accaggtgca caagggtcat
    16981 agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca acactgacac
    17041 cagcctctcg acaacgagcc ccatgcttac gcaactgtct ctctgagtta aagacgatat
    17101 ggtctgttat cccaagcaat tcctccatgt cctcatcctt gaaagctggt gcaaaaacat
    17161 ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca ctagctgtcg
    17221 tacctgatag atactggcta atcaagggat aagttttgta gagggaatat gccttctggg
    17281 caagcaagac cttgcaaccg gcctcttctt gtacatattg tagaatgcgg caattagctt
    17341 ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac ttcattagtc
    17401 caacatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca aagcttccat
    17461 gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc aagttccgtt
    17521 catgactaat tttgtcccaa tcatggctgg aacgcctgtc gtgtatgaaa ttgcttgtga
    17581 cccgacttct gcatagcact cttgatggtc gcaaacattg tagatgtaga tagtcttttc
    17641 aacgccatct ttgacacctg taaagataca tccaatattg gttttaccta cagtacgtgg
    17701 gccaaggcta gcaggatctg gaagcaaggc tttcaaaaat tgaattggaa caatttcttg
    17761 gccgttaaag ttaatggtat ccgtacgaag gagtccaaca ttttcaagac atttcatgtg
    17821 cgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgacac ctggaatgtt
    17881 cttggccaat gattcgattt cttcatggtg aaggagatac atatcttttt gtccaacttg
    17941 agggaaatca tactcacgct tgatagacat agcttcgact tcgacccatt tcccatcttc
    18001 ccagtaagaa cctggcgcag aaacctcacg gagattaatt tctgggttga agttggttgc
    18061 aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat ggatttcatc
    18121 aaaatagtgt ttgagggcat aagctgaaaa gacactggtt acacctgggt caaaaccaga
    18181 accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc actgccatga
    18241 gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga tagcacgcca
    18301 ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt gaacacctgt
    18361 tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat tcaaaacagc
    18421 ttctggtttg tagctttcaa tcaaggtaat gacctcttct accttgtcag catcaagtgc
    18481 tgcagtttca attttagtac ttgttttgcc ttctagcttc gctttcaagt catcgcattt
    18541 tgacttggta cggctagcaa tcataatctc tgtaaacgtt tcgctatctt gacaaatctt
    18601 tgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac tcattttttt
    18661 ccttcctctt cttctaaaat gtcctcaaca tacttgggca acataaaggc tcccacgtgt
    18721 aagtttgcag tgtagtattc tgtgaaaagc tggcgttttt tccagccttc cttgtcaaaa
    18781 tctttgacag ggtggtattt tttcgatgca aatccaaaca accaatagcc agctgggcta
    18841 gttggaatat gggcctgata aacccgactg attggaaagg cttgattgac cttgcggtgc
    18901 atgcttcggc aggccgactc atcctcgtca aagaagggac tcccatgctg gtaaatcatg
    18961 atgccgtctt ccttcagagc tcgataacta ttgccgtaga attccttggt aaagagtcct
    19021 tccgtatggc caaatggatc tgtcgcatcg ttgatgataa tatcgtaatc atcttcgcag
    19081 tttcgcaaaa agcgtagccc attttggtag taaatggtaa cacgaggatc atctagccct
    19141 gcagcaaagt ctgggaaata ctcacgacag acctcgacca acatctcatc cggttccacg
    19201 atatcgattt gttccagttc aggatagagt gttaatactt gggcaacacc gccgtcacca
    19261 cccccaataa ccaatacttt ctttggattt gggtggacag ccatgggaac gtgaacggtc
    19321 atttcattgt agacgaaatc atccgcatct gagaacaaga catggccatt taaaatcagt
    19381 attttcccaa aagctggcgt atccaagact tcgatatcct gccattcact ttttccagcg
    19441 taaagttgct tggctgttct cagagacaat ttgacatctg gagtatgaac ttcagaaaac
    19501 cataaatcca tctatttctc cttatattat atttagcgca gttagctact gccaaagccc
    19561 aagtggtata cttggaataa gccactgtgg attagttcat ttcctccaat cgcttgacga
    19621 tttccgaaag gatctaacca cagctttttg tttcaattgg taatgagttt gataacgcat
    19681 agacgtttta tctcgtctaa ggtaacaagg caaaaagttc gtggataacc aagtagactt
    19741 gaaatactac atctggaggt actcccatgc tttatgtggg cattgatgtc gctaaaaata
    19801 aacacgatgt tacagccttg aatgttc
//
LOCUS       DAVBCR010000036        19675 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCR010000036 DAVBCR010000000
VERSION     DAVBCR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.036"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            50..169
                     /locus_tag="VQ508_001195"
     CDS             50..169
                     /locus_tag="VQ508_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HET2490200.1"
                     /translation="MIGIVFAIFLDFPVEYGISLGLLNGIVLGSLIVYKNNKN"
     gene            203..931
                     /locus_tag="VQ508_001196"
     CDS             203..931
                     /locus_tag="VQ508_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490201.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            924..1661
                     /locus_tag="VQ508_001197"
     CDS             924..1661
                     /locus_tag="VQ508_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490202.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1674..2384
                     /locus_tag="VQ508_001198"
     CDS             1674..2384
                     /locus_tag="VQ508_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490203.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFSLLPKLIKLKEQNLV"
     gene            2927..3148
                     /locus_tag="VQ508_001199"
     CDS             2927..3148
                     /locus_tag="VQ508_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490204.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3665..3904
                     /locus_tag="VQ508_001200"
                     /pseudo
     CDS             3665..3904
                     /locus_tag="VQ508_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4048..5277
                     /locus_tag="VQ508_001201"
     CDS             4048..5277
                     /locus_tag="VQ508_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2490205.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5297..7993
                     /gene="ppc"
                     /locus_tag="VQ508_001202"
     CDS             5297..7993
                     /gene="ppc"
                     /locus_tag="VQ508_001202"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2490206.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    8076..8293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8406..9410
                     /locus_tag="VQ508_001203"
     CDS             8406..9410
                     /locus_tag="VQ508_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2490207.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9479..10345
                     /locus_tag="VQ508_001204"
     CDS             9479..10345
                     /locus_tag="VQ508_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490208.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10342..11100
                     /locus_tag="VQ508_001205"
     CDS             10342..11100
                     /locus_tag="VQ508_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490209.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            11190..12950
                     /gene="dnaG"
                     /locus_tag="VQ508_001206"
     CDS             11190..12950
                     /gene="dnaG"
                     /locus_tag="VQ508_001206"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2490210.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12953..14062
                     /gene="rpoD"
                     /locus_tag="VQ508_001207"
     CDS             12953..14062
                     /gene="rpoD"
                     /locus_tag="VQ508_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2490211.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            14076..14405
                     /locus_tag="VQ508_001208"
     CDS             14076..14405
                     /locus_tag="VQ508_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2490212.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14520..15563
                     /gene="cpoA"
                     /locus_tag="VQ508_001209"
     CDS             14520..15563
                     /gene="cpoA"
                     /locus_tag="VQ508_001209"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2490213.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15579..16904
                     /locus_tag="VQ508_001210"
     CDS             15579..16904
                     /locus_tag="VQ508_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2490214.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16935..17111
                     /locus_tag="VQ508_001211"
     CDS             16935..17111
                     /locus_tag="VQ508_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2490215.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            17170..17304
                     /locus_tag="VQ508_001212"
     CDS             17170..17304
                     /locus_tag="VQ508_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2490216.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17368..18678
                     /gene="obgE"
                     /locus_tag="VQ508_001213"
     CDS             17368..18678
                     /gene="obgE"
                     /locus_tag="VQ508_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2490217.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18688..18846
                     /locus_tag="VQ508_001214"
     CDS             18688..18846
                     /locus_tag="VQ508_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490218.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         6167 a         3271 c         4192 g         6045 t
ORIGIN      
        1 gaagaaagta aatgaatatg taatttcaac agcagcctca ctaggggtga tgattggaat
       61 agtatttgca atttttttag attttccagt tgaatatggt atttctttag gtttgttgaa
      121 tggaatagta ttgggttcgc tgattgttta caaaaacaat aagaattaag cataattttt
      181 tgctgtaaac taaggagtag agatggctat agttgaaatt ataaatctaa caaaaagctt
      241 taaagatatt gaagttattc ataacacttc attttattta aataagggaa aagtatatgg
      301 ttttgttgga ccaaatgggg ctggaaaaac tacaattatc aaaatgattc tagggatttt
      361 aaaaccagat tctgggaaaa taactatttt taatcaaacc gtagaacaga attccaaaaa
      421 tatattatct aggataggat tagttttggg accttcattt tatggtcatc ttgatgctta
      481 taaaaatttg aagttaatag caaatatgaa agggcttagt ttagatactg aaagacttaa
      541 tgaatatcta tcaatggtag gattaaaaga tgttaaaaag aaaaaagtga aaaatttttc
      601 aatgggcatg aaacaacgtc taagtatagc tgcatcattg ttaggtagtc cagaaatatt
      661 aatttgggat gaaccaatca atggacttga tccacagggt gtaattgaaa ttagaagttt
      721 aatccgtttt ctacaggaga aaaaaggtat tacattttta atatcaagtc atattcttag
      781 tgaactggat aaagtgatat ctgatattat aattataaat tatgggaaag ttgaattttt
      841 tggttcttgc cactaccttt tacaaaagta taattgtcga aatctggaag aagcctactt
      901 agcttgttta gctggaggtg aatatgatta aattagagtt tctaaagcag aaaaaaagca
      961 tcttatggtt tgttcttatt tttccaataa ttctgaatgc tttgttatat atagacttaa
     1021 cttttaggta tcgaggttat ttattggtgc atcaaaatga acttgcttta tctaattggc
     1081 agttaatttt taaagaacaa acaatatttt atttttctga actattttat ttagttctat
     1141 ctttaataat ttacgaagtg tttgcagtag aatttaaaaa tgatgcatgg ttaaccgtaa
     1201 tttcattgcc atttagaaac aagtacacaa taaattctaa attactcatt acagtggttt
     1261 acacatttac tttttggttg tctgattata ttagcttata cgttattggg aaggctattg
     1321 ataacagttt agagattggt ttgattttct ttttaaaaac atttaccatt caattaattt
     1381 cctcgttgat gataatgtta ttatattttc taactttagt acttataagg aaaatttctg
     1441 gaataatacc aattggtatt ataatgatga ttttaacaat tagcatatac tacaacgatt
     1501 ataattttaa gatttactta ccatttactt atctttctca tgcttttaga gtgactgaaa
     1561 gtcaatttta tatgatttta ctatctaata taataatcat cgtacttttt tatatattaa
     1621 ttcgtaaatt gaacgaaaga agttttgaga tgaaacttta ataggtaata aaaatgaagt
     1681 tacttaaaaa tgaattgatt aagagtaaaa tatttttatt tattattgtt gacatctgta
     1741 ttcagattct agtcattcta gctataaaaa catatatttt agatatttcg gcaccatact
     1801 cagagcttga ttataataag tattggtatg ttttacacgc tttaatatat atgccaatga
     1861 tttttcctat acaaattttg tatcaaaatc taagagaggc attgattgaa gataataata
     1921 atggttggaa tatcatggtg attaacacta ataatctcgt gaaaatcatc tatataaaag
     1981 taacgataaa tatcgtgaga tgttttattt gttattttgt ttatacaatt ttttctttaa
     2041 ttcaattagg tggtatggga actgatatgt tattaaccaa tataatattt cccaatataa
     2101 tgtcatttct gttattttta cctatagcta tctttatgca aatttgctgt ataagatttg
     2161 attccatttt agcaaaggcc ttacctaata ttttgctgat acttattgtt ttaatcacgt
     2221 ttcagagtga ttggaatata tttatacctg caacttatta ttatacagaa attcagtcaa
     2281 ctacaaactt aggtattaaa ttattagttt gtatctggat tatgggattt gaattttccc
     2341 ttcttccaaa actaataaaa ttgaaagaac aaaatctagt ttaaatgttg ctcgaaaatt
     2401 aaataattga atacgattaa caatgaaaca atacaacgtg agtcatgact tcaaagtaat
     2461 tgattttatg attctggttt cttacgctta attaagcaag ttgtattcac tttatcaaat
     2521 tgttattact ctaaaaagcg tctaacagct atagagtgtc tgtttgcttc tctttacttt
     2581 tattttaaat aattcattaa ccttccgtaa gtgcccaata ataaggtgtg ttgaagaagt
     2641 ctgagataaa atagttctca gtcacaaaaa aagctataga ttcccattgg tgaacagctc
     2701 cctgtcaagt agacagtaaa ataatataaa atcttaagcg acctgattcc tgaattccag
     2761 tggagtcagg ttgtttttta accgtctttc ttaatattgt ctcacctata atagatatgc
     2821 ttatgagata tgtttaataa attaatttta gttcaaaatg tccagttagg acactttgaa
     2881 tgatttctta gttttatata ttatactttt ctcaggagaa agctagatgt acagacgttt
     2941 gagagatttg agggaagatc acgatttgac ccaaaaacaa atagctaaaa tactttcgtt
     3001 tacaaactca gcttatgcta aaattgaacg gggtgagcat acgttaacag cagatgtatt
     3061 ggtaactctc tcaaactttt acgatgtcag tacagactac ctattggaat tgacagattt
     3121 tcctgataaa attcgcttta gaaaataatc tcctcaattt catagagtgt gaaaatgagg
     3181 agatttttta tttgttcttt gaaaattaaa tagtccaaaa tggtactttc ctcatttgtg
     3241 gagcaaattt gaatggctcg ccatgataag agcgatttta aagccatcaa taaaatagag
     3301 cgatacttta tatgccatga tacaaatgat atacaatgat acttctgacc gttcaggctg
     3361 ccatcgtaaa agagcagcaa gtgaaattct tatgatgact tcatcagtca tgccacgttg
     3421 aatgtgtgag tttgttagag aaacgcaatt aatcctcaaa aggttccccg aaccttttga
     3481 gttccacaga cgcatcacgt ggagtgtgta agcttgttgc taaaagcgta aaaaccttgg
     3541 aacgaaagga ataatagact tcctgcgaaa caaaaataca atacaataaa actatggatg
     3601 atgaagcaag taaacaatta agcgatagcc gtttcaagag ccttgtaggt gttcagcgca
     3661 cgagatgtta gctgtgttaa aaacagctta tcaacgcaac gtaaacgcgc aaaaggtgga
     3721 cgaaaaacca aattaagcct agacgatctc ctcatggtaa ctattcaata catgcgagaa
     3781 tagcgcactt atgaacaaat tgcggctgat tttggcattc acgaaagcaa cataattagt
     3841 cggaggtcaa tggattgaag caactcttat tcaaaatggt tttacgattt caaattctgc
     3901 cttaattctg taaaaacagt aaaattcgaa ggattgtaag gtaagagttt ttttctttct
     3961 gaaaaaatgg tataatagca atcaaaacta gaaaataaaa cggaatttgg aacagatttg
     4021 tctgtatcct agtagagtgg tgatactatg aagattagta agaggcactt attaaattat
     4081 tccatcttga ttccctactt gcttttatct attttgggct tgattgtggt ctattcgacc
     4141 accagtgcta ttttaattga agaaggcaag agcgccttgc agttggttcg aaaccaagga
     4201 atcttttgga ttgttagttt gatactgatt gccttaattt ataaattgag actagatttt
     4261 ttgagaaatg agcgactaat cattttagtt atattaatag aaatgctttt attgttcttg
     4321 gctcgtttta ttggtatttc agtaaacggg gcatacggtt ggatttcggt tgcaggagta
     4381 actattcagc cagctgagta cttaaaaatc attattattt ggtatttagc tcaccgattc
     4441 tccaaacagc aagaagaaat agctacttat gattttcaag ttttgactca aaatcaatgg
     4501 cttccccgtg cttttaatga ttggcgattc gttctcctag ttctgattgg aagtttggga
     4561 attttccctg atttaggaaa tgcgactatt ttagtcttgg tttccttgat tatgtataca
     4621 gttagtggaa tcgcttatcg ctggttttca accattctgg cgctcgtatc tgccgcttct
     4681 gtctttgtct tgaccactat cagcctaatc ggtgttgaga ccttttcaaa aattccagta
     4741 tttggctatg tagccaaacg ctttagtgcc ttttttaatc cttttgccga tcgtgctgat
     4801 gcaggtcacc agttagctaa ttcttatttt gccatggtca atggtggttg gtttggtcta
     4861 ggtcttggaa actcgattga aaaacgaggt tatttgccag aagctcatac agactttgtc
     4921 ttttctatcg tgattgaaga atttggcttt gttggtgcca gtcttatttt agctctcttg
     4981 tttttcatga ttttgcggat tatcttggtc ggtattcgag cggagaatcc tttcaatgcc
     5041 atggttgcac tcggtgtcgg agggatgatg ttggttcagg tatttgtcaa tatcggaggg
     5101 atttcgggct tgattccatc tacaggagta accttcccct tcttatccca gggtggaaat
     5161 agtcttctag tcttatcagt ggcagtagcc tttgtcttaa atattgatgc cagtgaaaaa
     5221 cgcgctaagt tgtaccgaga attggaaaat caaccaatga accttctgtt gaagtaggat
     5281 aaagaaagga tagtttatgt ctcttcaaaa attagaaaat tctagtaata aaagtgttgt
     5341 gcaagaagaa gtcttgattc taacagaatt actggaagat attactaaaa atatgcttgc
     5401 cccagagacc tttgaaaaaa taatacagtt gaaagaatta tcaacgcagg aagattatca
     5461 aggtctaaac cgtctagtga ctagcttatc aaatgatgaa atggtctata tttcacgcta
     5521 tttctctatc ttgcctcttt tgattaatat ttcagaggat gtggatttag cttatgaaat
     5581 caatcatcaa aataatattg atcaggacta tttaggtaaa ttatctacaa cgattaaatt
     5641 ggtagcagaa aaggaaaatg ccgttgagat cctagaacac ttgaatgttg ttccggtttt
     5701 gacagcccat ccaacacaag tgcaacgcaa aagtatgttg gatttaacaa atcatattca
     5761 tagtcttttg cgtaaatacc gtgatgttaa gttggggttg atcaataaag ataaatggta
     5821 caatgatttg cgtcgttaca tcgaaattat catgcagaca gacatgattc gtgagaaaaa
     5881 attaaaagtg actaacgaaa tcacgaatgc tatggaatat tataacagct cctttttgaa
     5941 agctgtacct catttgacga cggagtataa gcgcttagcg caagcgcatg gtctgaattt
     6001 aaaacaggct aaaccaatca ccatgggtat gtggataggt ggtgaccgtg atggaaatcc
     6061 atttgttaca gcaaagacct tgaagcagtc tgcactcact caatgtgaag tcatcatgaa
     6121 ctactatgat aaaaagattt accaacttta tcgtgaattt tctctttcaa ctagcattgt
     6181 caacgtcagc aagcaagtca gagaaatggc tcgtcaatcc aaggataact cgatttaccg
     6241 cgaaaaagag ctttaccgtc gtgccttgtt tgatattcaa tcaaaaattc aggcaactaa
     6301 aacctatctg attgaggatg aagaagttgg gactcgttat gaaaccgcca atgatttcta
     6361 caaggatttg attgccattc gagattctct actagagaat aagggcgagt ccttgatttc
     6421 aggtgatttt gtggaattat tgcaggcagt agagatattt ggtttttacc tagcatcaat
     6481 tgatatgcga caagactcta gcgtctatga agcctgtgtg gcagaactct tgaaatcagc
     6541 aggaattcat tctcgttata gcgagttgag cgaagaagaa aagtgtgacc ttcttttgaa
     6601 agaattagaa gaagatcccc gaattctttc tgcgactcac gcagaaaaat cagaattatt
     6661 agcaaaagaa ctagctattt ttaagacggc tcgtgttttg aaagataagt tgggagatga
     6721 tgtcatccgt cagaccatca tttcacatgc aaccaacctt tctgatatgc tagaattagc
     6781 tattctgtta aaagaagtag gactggtgga tacggaaagg gcgcgtgttc agattgttcc
     6841 cctttttgaa acaattgaag acttggatca ttcagaggaa acaatgagaa aatatctttc
     6901 tcttagcctt gccaaaaaat ggattgactc acgaaataac taccaagaaa tcatgcttgg
     6961 ctactctgac agtaataaag atggcggtta cttgtcatca tgttggaccc tctacaaggc
     7021 tcaacaacaa ttgactgcta ttggagatga atttggcgtt aaggttacct tcttccatgg
     7081 tcgtggtggt actgtcggtc gtggtggtgg gccaacctat gaagccatta catctcaacc
     7141 gctcaagtct atcaaggatc gtatccgttt gacggagcag ggtgaagtaa ttgggaataa
     7201 atacggtaac aaagacgccg cttactataa ccttgaaatg ctagtatcgg cagctattaa
     7261 ccgtatgatt actcagaaga agagcgatac caatacccca aatcgttatg aagccattat
     7321 ggatcaagta gtggaccgta gttacgatat ctaccgtgat ttggtctttg gtaatgagca
     7381 tttctatgat tatttcttcg agtcaagtcc aatcaaggct atttcaagtt ttaatattgg
     7441 ttctcgtcca gccgctcgta agactattac tgaaatcggt ggtttgcgtg ccatcccttg
     7501 ggtattctca tggtcacaga gtcgtgttat gttccctgga tggtacgggg ttggttcaag
     7561 cttcaaggaa tttatcaata aaaatccaga gaatattgct atcttacgag atatgtacca
     7621 aaattggcct ttcttccaat cgcttctttc aaatgttgat atggttttgt caaaatcaaa
     7681 tatgaatatt gcttttgaat atgctaaact ttgtgaagac gagcaagtta aggccatcta
     7741 tgagattatt ttaaatgaat ggcaagttac taagaacgtt atcttggcta ttgaaggaca
     7801 tgacgaactc ttagctgaca atccatatct aaaagctagt ctggattacc gtatgcctta
     7861 ctttaatatt ctcaactata ttcagttaga gttgattaaa cgccaacgtc gtggagaatt
     7921 gtccagtgat caagaacgat tgattcatat caccatcaac ggaattgcga caggattgcg
     7981 taattcaggt tgataatttt caagagtgaa tgctaaaagt gaatatcaaa aaaattctaa
     8041 tagactattg acaagtagtt taaaaatgat ataatttaac cattcagaaa agtaatcata
     8101 caaacttttt agagagtctg tggtagctga aaacagataa gtggcgatga tgaaaattgg
     8161 gctgaatgct atttagaatt tgaaattata aaaattcggt aagcacacct tacagtgcat
     8221 ctcgttattg cgagactgag cgatagggaa attccctata attgaggtgg taccgcgcat
     8281 cgacgtcctc acacaagttt tttgtgtgag gatttttttg atggaggtta gtatggaaag
     8341 aaaacgatgg cgtcgcttgt ttagataagt gaaatatgtt aaaggaaata aaaaggagaa
     8401 acagaatgaa aaataaacgt ttaattggaa ttattgctgc attagcagtc ttagtagcag
     8461 gaagcttgat ttattcttca atgaataaat cagaagctca gaataataag gatgagaaga
     8521 aaataaccaa gattggtgtg cttcaatttg tgagccatcc atcccttgat ttgatttata
     8581 aagggatcca agatggactt gcagaagaag gatataaaga tgatcaagtt aaaattgatt
     8641 ttatgaactc agaaggtgat caaagtaagg ttgcgacaat gagtaaacaa ttggttgcaa
     8701 atgggaatga ccttgtggtt ggtattgcaa caccagcagc ccaagggttg gctagtgcaa
     8761 caaaagacct accagttatc atggccgcta ttacagaccc aattggtgct aacttggtta
     8821 aagatttgaa aaaaccaggt ggtaacgtta caggggtatc tgaccacaat ccagctcaac
     8881 aacaaattga actcatcaag gctctgacac cgaatgtgaa aacaatcgga gctctttact
     8941 caagtagcga agacaattca aaaacacagg ttgaagaatt taaggcttat gctgaaaaag
     9001 caggtctgac agtggaaaca tttgcagttc cttcaacaaa tgaaattgcc tcaactgtca
     9061 ctgttatgac tatcaaggta gatgctattt gggttccaat tgataacacc attgcatcag
     9121 gatttccaac ggttgtctct agcaatcaaa gttctaagaa accaatttat cccagtgcga
     9181 cagctatggt agaagtaggt ggtttggcat cagttgtaat tgaccaacat gaccttggtg
     9241 tggcaacagg taaaatgatt gtgcaagtct tgaaaggtgc aaaaccagcc gataccccag
     9301 tcaatgtctt ttcaactggt aagtcagtca tcaataaaaa aatagcacaa gaactaggta
     9361 ttactattcc tgagtctgtt ctcaaagaag caggacaagt catcgaataa ataaggtagg
     9421 gaggaggaga ctcttcccat tttttaaaaa gatgaatata gaaaggatta aataaaatat
     9481 gattgtttcc attatttctc aaggatttgt ctgggctatt ctaggtctgg gaatctttat
     9541 gacatttagg attttaaact ttccagatat gacgacagaa ggttccttcc ctcttggggg
     9601 agctgttgct gtcactttga taaccaaagg cgtgaaccca tttttagcga cacttgttgc
     9661 tgtaggagca ggttgtttgg ctggaatggc agcaggcctt ctttatacaa aagggaagat
     9721 cccaaccttg ctctcaggga ttttggtgat gacttcttgt cactcaatca tgctcttgat
     9781 tatgggacgt gcgaatttag gcctgcttgg aaccaagcaa attcaggatg ttttgccttt
     9841 tgattcggat ttgaatcaac tcttgacagg tctcatcttt gtgagcattg ttattgctct
     9901 catgctcttt ttcttggaca ctaaactcgg acaagcctat attgctacag gggataatcc
     9961 tgatatggct agaagtttcg ggattcatac tggacgcatg gagctcatgg gcttggtctt
    10021 atcaaatggt gtgattgccc ttgcaggtgc cctcattgct cagcaagaag gctatgccga
    10081 tgtgtctcga gggatcgggg ttatcgttgt ggggcttgca agtttgatta ttggagaagt
    10141 tattttcaag agtttgagct tggcagagcg tttggttact atcgttgtag gttctatcgc
    10201 ttatcaattt ttagtgtggg cagttatcgc acttggcttt aatacaagtt accttcgttt
    10261 atacagtgcc ttgattttag cagtctgcct catgattcca acatttaagc aaacaatctt
    10321 gaaaggagcc aagttaagca aatgacagca attgtagaat taaaaaatgc aaccaaaatt
    10381 gttaaaaatg gctttgatga agaaaagatt attttaaatg atgtttcctt agaaattttt
    10441 gaacgggact ttatcacgat tttgggcgga aatggtgctg gaaaatcaac cctctttaac
    10501 actatagcag ggaccttatc actaactagt ggaactatcc gtattttagg tgaagatctc
    10561 actaagtttt cacccgagaa gcgtgccaag tacctgtctc gtgtcttcca agatccaaag
    10621 atggggacag ctccccgtat gacggtcgct gaaaatcttt taatcgccaa gtttcgtggt
    10681 gagaagcgtg gattgttacc acgacgcttg actagctata aggatgaatt tcaggcaacc
    10741 attgaaaaag taggaaatgg tcttgagaaa cacttgaata caccgattga gttcttatca
    10801 ggtggacaaa gacaggcttt gagtctcttg atggcaacct tgaagcgacc tgaattactc
    10861 ctgttagatg agcatactgc tgccctggat ccaaagacta gtgttgcttt gatggaattg
    10921 acagatgaat ttgttaagaa agatcagcta acagccctta tgattactca tcatatggaa
    10981 gatgctctca aatacggcaa tcgcttgatt gtcatgaaag aaggacggat tatccaagat
    11041 ttaaaccaag aagaaaaagc aaaaatgaaa atctctgatt attatcaact ctttgaataa
    11101 agtagggaaa atgagtgaaa tggtttactt tttttctgaa ataaagtata ctatataaag
    11161 taaactatga taacatggag gtattgtgta tggttgacaa acaagtcatt gaagaaatca
    11221 aaaacaatgc caacattgtg gaagtcatag gagatgtgat ttctttacaa aaggcaggac
    11281 ggaactatct agggctctgt ccttttcatg gtgaaaaaac accttctttc aacgttgtag
    11341 aggacaagca gttttaccac tgttttggtt gtggtcgctc aggtgatgtc tttaaattca
    11401 tcgaggagta ccaaggggtt acctttatgg aggctgtcca aatcttaggt cagcgtgtcg
    11461 ggattgaggt tgaaaaaccg ctttatagtg aacagaagcc agcctcgcct caccaagctc
    11521 tttatgatat gcacgaagat gcggctaaat tttaccatgc tattctcatg acaacgacta
    11581 tgggcgaaga ggccagaaat tacctttatc agcggggttt gacagatgaa gtgcttaaac
    11641 atttttggat tggtttagca cctccagaac gaaactatct ctatcaacgt ttgtctgatc
    11701 agtatcgtga agaggattta ctggattcag gcctgtttta tctctcggat gccaatcaat
    11761 ttgtagacac ctttcacaat cgcattatgt ttcccctgac aaatgaccaa ggaaaggtca
    11821 ttgccttctc aggtcgtatc tggcaaaaaa cggattcaca aacttctaag tataaaaaca
    11881 gccgatcgac tgcaattttt aacaaaagtt acgaattata tcatatggat agggcaaaaa
    11941 aatcttctgg aaaaactagt gagatttacc tgatggaagg attcatggat gttattgcag
    12001 cctatcgggc tggaatcgaa aatgctgtgg cgtcgatggg aacggccttg agtcgagagc
    12061 atgttgagca tctgaaaagg ttaaccaaga aattggttct tgtttacgat ggagataagg
    12121 ctgggcaagc cgcgatattg aaagcattgg atgaaattgg tgatatgcct gtgcaaatcg
    12181 ttagcatgcc tgataacttg gatcctgatg agtatctaca aaaaaatggt ccagaagact
    12241 tggcctatct attaacaaaa actcgtatta gtccgattga gttctacatt catcagtaca
    12301 aacctgaaaa cagtgaaaat ctgcaggctc agattgagtt tattgaaaaa atagctccct
    12361 tgattgttca agaaaagtcc atcgctgctc aaaacagcta tattcatatt ttagctgaca
    12421 gtctggcgtc ctttgattat acccagattg agcagattgt taatgagagt cgtcaggtgc
    12481 aaaggcagaa tcgcatggaa agaatttcca gaccgacgcc aatcaccatg cctgtcacca
    12541 agcagttatc ggctattatg agggcagaag cccatctact ctatcggatg atggaatccc
    12601 ctcttgtttt gaacgattac cgtttgcgag aagactttgc atttgctaca cctgaatttc
    12661 aggtcttata tgacttgctt ggccagtatg gaaatcttcc tccagaagtt ttagcagagc
    12721 agacagagga agttgaaaga gcttggtacc aagttttagc tcaggatttg cctgctgaga
    12781 tatcgccgca ggaacttagt gaagtagaga tgactcgaaa caaggctctc ttgaatcagg
    12841 acaatatgag aatcaaaaag aaggtgcagg aagctagcca tgtaggagat acagatacag
    12901 ccctagaaga attggaacgt ttaatttccc aaaagagaag aatggagtaa taatggcaac
    12961 aaaacaaaaa gaagtaacaa catttgacgt acaggtagca gaatttatcc gtaatcataa
    13021 gcaaaaaggg acagcaacag atgatgaaat caatgcaagt ctggttattc cttttacctt
    13081 ggacgctgat gggattgaag atctcttgca acggattcag gatgcaggga tttctatcac
    13141 agataacgaa ggaaatccaa gtgcgcgtgt tcttagcaat gaagaagaac cagaactcag
    13201 cgatgaggac ttgattggat caacttctgc taaggtcaat gaccctgtcc gtatgtactt
    13261 gaaagaaata ggggtcgttc ctctcttgac caatgaagag gagaaagagt tggcactggc
    13321 tgttgaagct ggcgatatcg aagccaaaca acgtcttgcg gaagccaatc ttcgtttggt
    13381 tgtttccatt gccaaacgct atgtcggtcg tggcatgcag ttccttgact tgattcaaga
    13441 aggaaatatg ggcttgatga aggcggttga caagtttgac tattctaaag ggttcaagtt
    13501 ttcaacttat gcaacttggt ggattcgtca ggctatcact cgtgctattg cggaccaagc
    13561 tcgtaccatc cgtatcccag ttcacatggt tgaaactatc aataaattgg ttcgtgaaca
    13621 gcggaatctc cttcaagaat tggggcaaga tccgacacca gaacagattg ctgaacgaat
    13681 ggatatgaca cctgataagg ttcgtgaaat cttgaagatt gcccaagaac cagtatctct
    13741 tgaaactcct atcggtgaag aggacgatag ccaccttgga gactttatcg aagatgaagt
    13801 gattgaaaat ccagtggatt atacgactcg tatcgtcttg cgtgagcaat tggatgaaat
    13861 cttagatact cttacagacc gtgaagaaaa tgttctgcgt ctacgttttg gactagatga
    13921 tggaaaaatg cgcacacttg aagatgtggg gaaagtcttt aacgtaactc gtgagcgtat
    13981 ccgtcagatt gaagcaaagg ctttgagaaa actacgccaa ccaagtcgca gcaaaccgct
    14041 tcgtgatttt attgaagact aagagtgagg aaaatatggc ttatacagaa gagcaaattg
    14101 aaaacatcaa aacacggatt ttaacagcct tggaagaagt catcgaccct gagttgggaa
    14161 tcgatattgt caatcttggt ttgatctatg agattcgttt tgacggtgac acagggcaaa
    14221 cagagattga catgactttg acaactatgg gttgtccctt agcagacctt ttgacagacc
    14281 agatttatga tgctatgata gaggttccag aagtaacgga tactgaggtt aaattagtct
    14341 ggtatccagc ctggacagtt gaaaaaatga gtcgctatgc ccgcattgcc ctaggcatta
    14401 agtaaacata taaaagcatg tgagagacta gaggaaaagc gaggaaattt cccctctttt
    14461 cctctagtct ctcctttctt ttgctgattt tattcaaaga aaatgatata atagtagtta
    14521 tggagaaaaa gaaattacgc atcaatatgt tgagttcaag tgagaaagta gcaggacagg
    14581 gagtttcagg tgcttaccgt gaattagttc gtcttcttca ccgtgctgcc aaggaccaat
    14641 tgattgttac agaaaatctt ccaatcgagg cagatgtgac tcactttcat acgattgatt
    14701 ttccctatta tttatcaacc ttccaaaaga aacgctcagg gagaaagatt ggctatgtgc
    14761 atttcttgcc agctacactt gaaggaagtt tgaaaattcc atttttctta aagggaattg
    14821 tgaaacgcta tgtattttct ttttacaacc ggatggagca cttggttgtg gtcaatccta
    14881 tgtttattga ggatttggta gcagctggta ttccacgtga aaaagtgacc tatattccta
    14941 actttgtcaa caaggaaaaa tggcatcctc taccacaaga agaggtagtc agactgcgca
    15001 cagatcttgg tcttagtgac aatcagttta tcgtagtagg tgctgggcaa gttcagaaac
    15061 gtaaagggat tgatgacttt atccgtctgg ctgaggaatt gcctcagatt acctttatct
    15121 gggctggtgg cttctctttt ggtggtatga cagatggtta tgaacactat aagaaaatta
    15181 tggaaaatcc ccctaaaaat ttgatttttc caggcattgt atcgccagag cggatgcgcg
    15241 aattgtatgc tctagcggat cttttcttgt tgcctagtta caatgagctc tttcctatga
    15301 ctattttaga agctgcgagt tgtgaggctc ctattatgtt gcgtgattta gatctctata
    15361 aggtgatttt ggagggaaat tatcgggcga cagcgggtag agaagagatg aaagaggcta
    15421 ttttggaata tcaagcaaat cctgctgtct taaaagatct caaagaaaag gctaagaata
    15481 tttccagaga gtattctgaa gagcatctgt tacaaatctg gttggacttt tatgagaaac
    15541 aagccgcttt agggagaaag taaaaagtga ggtaatctat gcgaattggt ttatttacag
    15601 atacctattt tcctcaggtt tctggtgttg cgaccagtat tcgaaccttg aaaacagaac
    15661 ttgaaaagca gggacatgct gtttttatct ttacgacgac agataaggat gtcaatcgct
    15721 acgaagattg gcaaattatc cgcattccaa gtgttccttt ctttgctttt aaggatcgtc
    15781 gctttgccta ccgaggtttt agcaaggcac ttgaaattgc taaacagtat cagctagata
    15841 ttatccatac tcagacagaa ttttctcttg gcctgttggg gatttggatt gcgcgtgaat
    15901 tgaaaattcc agtcatccat acctatcaca cccagtatga agactatgtc cattatattg
    15961 ctaaggggat attgatccgg ccgagtatgg tcaagtatct ggttagaggt ttcctgcatg
    16021 atgtggatgg ggttatttgc cctagtgaga ttgtccgtga cttgctatct gattataagg
    16081 tcaaggttga aaaacgggtc attcctactg ggattgaatt agccaagttt gagcgtccgg
    16141 aaatcaagca ggaaaatttg aaagaactgc gtagtaaact agggattcaa gatggtgaaa
    16201 agacgttgct tagtctttcg agaatctcct atgaaaaaaa tattcaagca gttttagcag
    16261 cctttgctga tgttctgaaa gaggaagaca aggttaaact ggtagtagct ggggatggcc
    16321 cttatctgaa tgacctcaaa gagcaagccc agaacctaga gattcaagac tcagtcatct
    16381 ttacagggat gattgctcct agtgagacgg ctctttacta taaagcggcg gatttcttca
    16441 tttcggcatc gacaagcgaa acgcaaggtt tgacctactt ggaaagctta gccagtggaa
    16501 cacctgtcat tgctcacgga aatccttatt tgaacaacct catcagtgat aaaatgtttg
    16561 gagccttgta ctatggagaa catgatttgg ctggtgctat tttggaagcc ctgattgcaa
    16621 caccagacat gaatgagcat accttatcag agaaattgta tgagatttca gctgagaact
    16681 ttgggaaacg agtgcatgag ttttatctgg atgccattat ttcaaataac ttccagaaag
    16741 atttggctaa agatgatacg gtcagtcagc gtatctttag gacagttttg tatcttccgc
    16801 aacaggtggt tgctgtacct gtaaaaggat ctagacgcat gttgaaggct tcaaaaacac
    16861 agttgatcag tatgagagac tattggaaag accatgaaga atagaaagag gaacagctat
    16921 gaaaaaaaca attaatgaga agcggtcgtg ataaaaagat tgcgggtgtt tgtgctgggg
    16981 tggcccatta tctggatatg gatccgacta tcgttcaagt catttggggt gttcttactt
    17041 gctgttacgg agctggaatt gtagcttaca ttattttatg gattatcgca ccagtagcaa
    17101 ctgactattg aaaactagct caattcatgc taagataata gaaaatagaa tgtaacgaag
    17161 gagagaaaaa tggcatttgg agataatgga aatcgtaaaa aaactatgtt tgagaaaata
    17221 accttgttta tcgtgattat catgctagta gcaagtttat tgggaatttt tgcaactgca
    17281 attggtgccc tcagtaatct ataaaataga ttcaagaaaa tttagtgact gggatttccc
    17341 agcccttttt taaagtgaga agaaataatg agtatgtttt tagatacagc taagattaag
    17401 gtcaaggctg gtaatggtgg cgatggtatg gttgcctttc gtcgtgaaaa atatgtccct
    17461 aatggaggcc cttggggtgg tgatggtggt cgtggaggca atgtggtctt cgttgtagac
    17521 gaaggactac gtaccttgat ggatttccgc tacaatcgtc atttcaaggc tgattctggt
    17581 gaaaaaggga tgaccaaagg gatgcatggt cgtggtgctg aggaccttag agttcgagta
    17641 ccacaaggta cgactgttcg tgatgcggag actggcaagg ttttaacaga tttgattgaa
    17701 catgggcaag aatttatcgt tgcccacggt ggtcgtggtg gacgtggaaa tattcgtttc
    17761 gcgacaccaa aaaatcctgc accggaaatc tctgaaaatg gagaaccagg tcaggaacgt
    17821 gagttacaat tggaactaaa aatcttggca gatgtcggtt tagtaggatt cccatctgta
    17881 gggaagtcaa cacttttaag tgttattacc tcagctaagc ctaaaattgg tgcctaccac
    17941 tttaccacta ttgtaccaaa tttaggtatg gttcgcaccc aatcaggtga atcctttgca
    18001 gtagccgact tgccaggttt gattgaaggg gctagtcaag gtgttggttt gggaactcag
    18061 tttctccgtc acatcgagcg tacacgtgtt atccttcaca tcattgatat gtcagctagc
    18121 gaaggccgtg atccatatga ggattaccta gctatcaata aagagctgga gtcttacaat
    18181 cttcgcctca tggagcgtcc acagattatt gtagctaata agatggacat gcctgagagt
    18241 caggaaaatc ttgaagaatt taagaaaaaa ttgtctgaaa attatgatga atttgaagag
    18301 ttaccagcta tcttcccaat ttctggattg accaagcaag gtctggcaac acttttagat
    18361 gctacagctg aattgttaga caagacacca gaatttttgc tctacgacga gtccgatatg
    18421 gaagaagaag cttactatgg atttgacgaa gaagaaaaag cctttgaaat tagtcgtgat
    18481 gacgatgcga catgggtact ttctggtgaa aaactcatga aactctttaa tatgaccaac
    18541 tttgatcgtg atgaatctgt catgaaattt gcccgtcagc ttcgtggtat gggggttgat
    18601 gaagcccttc gtgcgcgtgg agctaaagat ggggatttgg tccgcattgg taaatttgag
    18661 tttgaatttg tagactagga gactggtatg ggagataaac cgatatcttt ccgagatgcg
    18721 gatggtaatt ttgtttccgc cgcagacgtt tggaatgaaa agaaattgga agaactattt
    18781 aatcgtctca atccaaatcg tgccttgaga ttggcacgaa ctaaaaagga aaatccatct
    18841 cagtaaagaa gctaaaaaat cccgtgcctc atcagacacg ggattttgtg gtacgacagg
    18901 catgtatagc aaactgaatc tggaatagca cagcatatct tctaaaatat tgttataaat
    18961 tgatttgaat ttcaaaatta aattgtttga ttcttatctc aatttgttat agtatatctg
    19021 atgtcaaagt tctcggcgag tcaaatagcg attcccaagc ctgactatcg tgaggtagcg
    19081 gattaaaatg gtctggggat agaccgtttt aagtctgacg ctggaaataa gaattgtcag
    19141 aagaagggat agcgaaatcg tggctctacg aacaggaacg tgataataag gcgtatatag
    19201 cggataagag ggcctcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgcgtaaa
    19261 tcacgagagt aattgaattc gtactaagat tttctatttt cactgtaacc ttttaacgtc
    19321 cttatatctt gtatacacga ggaaagatgt acgacttatc ccgtgaggtc tatcactata
    19381 aagagaaaac gacagataga agtgatcctg agtcacggtt atctttaaaa tagattgtct
    19441 gataggacgg tatgtataaa acgcttctgt gaactgagag aagggggaga agttcttgct
    19501 aaaatttagt tgaacagccg tattccgata cttagataag agatctagtc ttagctccta
    19561 ctcagtttta ggggataaaa aaggggaaat agcgattcga gaaagattat actcttcgaa
    19621 aatctcttca aatcacgtca atatcgcctt gccgtatgtg taggatactg actac
//
LOCUS       DAVBCR010000037        18363 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCR010000037 DAVBCR010000000
VERSION     DAVBCR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.037"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..1879)
                     /locus_tag="VQ508_001215"
     CDS             complement(<1..1879)
                     /locus_tag="VQ508_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2490219.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(1873..2742)
                     /locus_tag="VQ508_001216"
     CDS             complement(1873..2742)
                     /locus_tag="VQ508_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2490220.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(2826..5471)
                     /locus_tag="VQ508_001217"
     CDS             complement(2826..5471)
                     /locus_tag="VQ508_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2490221.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(5562..6842)
                     /locus_tag="VQ508_001218"
     CDS             complement(5562..6842)
                     /locus_tag="VQ508_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2490222.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7013..9097
                     /locus_tag="VQ508_001219"
     CDS             7013..9097
                     /locus_tag="VQ508_001219"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2490223.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAT
                     SQNYRQLFDTETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKVCQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9137..10816)
                     /locus_tag="VQ508_001220"
     CDS             complement(9137..10816)
                     /locus_tag="VQ508_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2490224.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            11572..12801
                     /gene="arcA"
                     /locus_tag="VQ508_001221"
     CDS             11572..12801
                     /gene="arcA"
                     /locus_tag="VQ508_001221"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2490225.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12859..13875
                     /gene="argF"
                     /locus_tag="VQ508_001222"
     CDS             12859..13875
                     /gene="argF"
                     /locus_tag="VQ508_001222"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2490226.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14040..14987
                     /gene="arcC"
                     /locus_tag="VQ508_001223"
     CDS             14040..14987
                     /gene="arcC"
                     /locus_tag="VQ508_001223"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2490227.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15196..16707
                     /locus_tag="VQ508_001224"
     CDS             15196..16707
                     /locus_tag="VQ508_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2490228.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            16729..18060
                     /locus_tag="VQ508_001225"
     CDS             16729..18060
                     /locus_tag="VQ508_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2490229.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKCVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5147 a         4163 c         3811 g         5242 t
ORIGIN      
        1 aagtcgtata aatcgtcgaa gctgtcgcaa tggtatgcca ctggttagct gtagttgctg
       61 cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat ttcaacttcc agctcgtcga
      121 tgcggtcaag ctggccagcc agataaacct cgatacggct ttctgctcgt ttgatgcgtt
      181 gcaagagtcc gcccatacgg atgtcaactg tatccaaacc aaagaccttg ttttctttca
      241 gccattggtg actaaagagg gcatggaagt cttcaatttg gcttctaagt tctggtaatt
      301 cttgtctggc gatttgttgt aaactttctt tatcatccgc ttggtaggcc tgacgaatgc
      361 gtcgtcccac atctactttg ctacttaaaa tagcattcaa ctgggcctga gtttcaaaga
      421 gataagcata gtttccagct ttttctttaa tgttagcaag cgtctcagca gcctgagcga
      481 agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag aatcggacaa agaatatcct
      541 gataaaaaac atagcggttg ggattgatac cgctgagatt gcctggtagg tctggtaaga
      601 ggttggcaag gtcaatctgc ataaaatcct caaccgttag accagtattg gtcttgaagt
      661 gcgcagacaa accatctagg tcattgcgat agctgagttc tgcccagatt tgcaagcttg
      721 gtaggataga gaactgggca gtttcaccac cattgtctcc ccaacccgtt acgatgactt
      781 ctttaatctg attggcacgg caggctttat tagcctcgat agccactaga cggctaaaat
      841 ggttgtgagg tgtaaagcca atccacttcc aagctccccc tgcaaatgca aggtcatggc
      901 taatcttgtg atgattgcgg aaattacggt tgtatttttc ctcgctatcc tgataataat
      961 cccagtaaac cagagtcaca cggtctttga gacggtctag gtagacacga gtttcctctg
     1021 gaatttccac atcacggtcg tactggccat ccgctgacat gagtttgaag aacatatcac
     1081 tccacatctg gcagtggaaa ccatatttgt cagcaatatc cagcacgcgc tccaagtgtt
     1141 ggcacatgag gagactacga tccacaacac cgttcaaaat caggtagcgt cccaaaccaa
     1201 ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt cttcagttta gacaacgtgg
     1261 caaacatgcc atcaatcagg tcataaacct tttcttcgcc aatgagaaga atgtcctcta
     1321 catcacggag ctcctgcact tccttgacac cccatttgac aaaggccgac aagtgggcca
     1381 aggtctggat gcatggtaca aaggtcatgt caaactgttg agcataggct tcgatttcct
     1441 gcaactcctc tgctgaataa gctccacgga aatagccaaa gtaaggctgc ccttcaatct
     1501 ggtaagtgtc ttccatgtaa agttcaaagg ttgagtagcc catgagagcc aagacctcaa
     1561 tcatctgctt ggcagaagcc acattcagca ccgcatttcg agaacagtca accatgtaag
     1621 ccaaatcttc gtaagctgct tgttcctcaa tctctacttt atcagcttct gctagaactg
     1681 ttaccaacaa ggataaggca cgataaagtt ggtgaggttt gcggtaggtc aattgatagt
     1741 gaccttcctc accctggata gagatagagg cttggtcaga ctgagtgaca gccacttcca
     1801 catctggtag agaaatatga ccttttaaaa cctcaatagc ttgcgtttgt ttgggactaa
     1861 gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag ttgacaagag caccgtagag
     1921 attggcatct gcgtgatagg tgcaggcctg gataactggt gcgaccgtgt attcttcgta
     1981 ggtatcgaca aagttatcaa cagccttctt gacatcttgg ataaaatctg gattttgact
     2041 gatagagcca cccagactga tgacatctgg atcgatcaga tactggatat tgagcaagcc
     2101 ttgcgccaga ttgcggttca tgcgctcaat ggcttcttga caaaggatat taccagctgc
     2161 ggcctcttgg taaatcttgc gaccgtccca atcagtatga ccagattttt caatcacgta
     2221 tcgtaccata ttcccagttg atgctagttg cgaccagtta ttaagttttt cagcaggggc
     2281 aagggttgtc atgtagccaa attctccacc caagccgtgg cgacctcgat gaagtctacc
     2341 attgataatc atggctccgc caatccctgt cccaatcacg acacaggctg cattttcaag
     2401 atctggatga gctagtagtt cactgagtcc aacgcagttg gcatcatttt ctaaatggac
     2461 aggtagctga taagagctaa gcgcctcata ccaagaaaag ccatggatgt agggcaccgc
     2521 actgaagcca tcaatcacac ctgtctcttg attgactgca cctggaacgc tcatagcaat
     2581 cccactgtaa tcctgttctg acaagcgttg atctagccac gctagtaaat cctccaagtt
     2641 ttcaggcgtt gaaatacttg tcttatccag tattttccca tcaggagtca gactggcaaa
     2701 cttaatccca gtccctccga tatcaatcgt tgcaatggtc attgttttta ccataaaaag
     2761 gggcgaatca gcagttagtt cttacttttt cgcccctcct ttcttttgat gtttactttt
     2821 tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa tctcttgtgg atccaatggt
     2881 cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa tggtttgttg atgttctgat
     2941 atgtgcacat tttcttgact catattatag taacggaaga catagccttc ttcattttcc
     3001 gccactttaa aggctgttgg acaaacttgc ggtatgctga gaacagaatg gctcaagagg
     3061 ctaccagtcg caaccacgct tccttcctgt ctagcaagct gaaggctggt aaacggtgtc
     3121 tgcaaggctt tggcacgacg gaaagctgaa aagcgttctt gggcttggtg gcattcaagt
     3181 gcaaactcga cttcaaactc acgcaagcat tgtgcttctg gcgttgggaa gtagccccag
     3241 tcacctagct cacctgatgc acgcaaaatg gtcacggcaa tggtgttatc cccaaggatt
     3301 tcgtattcat tcaatccctt attggataca gtcacccctt tttcatcgtc atacagactg
     3361 acaaaagctt gttggtgttg aggattttca ggattttccc atgaagcagc tggtttgttt
     3421 ggtcgtgtca ccacctcata gatactttca gaatcattgc ttggacgcgt gttatgagtc
     3481 ttgaccaaga gacggatacg gtgatccttg gcagtgttag taaagcgagt cttgaagcgg
     3541 atttgtggat tgtcaacaaa gacaatcaac tcagtttcca gaggaatgtt tgtcaattct
     3601 tctgaccgtc cagcctcacg cttcataaac tcgatgatac cttgttgctc ttcttctagc
     3661 ttttcatccg cactgacagg cacggtcaat tcatgtttga gcaagatttt agcatagcga
     3721 tctgtgtttt ccaagacctc gtggccctta agctctgcaa agattggctc tgttcctttt
     3781 ggttggaaat agatatactc gtttccgatg tccccacggt cttcgaagcg gataaagtct
     3841 tcataggctt cgtgagttgt cttgtcatat actgtgatat tgtcatccac actcaccgtt
     3901 acgaatggtg tatcaatcac tccgttttgg taaataccct cacggtgttc ttgttttcct
     3961 tccagcaatt ggaaggttgt ccaagaaagc ggcgctaggt gaactggaat ggtcacgcgc
     4021 acttgtcgag caatacgagc ttggcggaac ttgtcttttg gtaaatcgta ctcaaaatta
     4081 gctccgaggt cttcgattgt agcctctaca ggacgaccat ccaagtcctc cacacggtaa
     4141 cttggtaaga taagagcagc catctttttg tagccttctg ttgggtgcaa ttccttgaaa
     4201 tcacaagtcg ccacatcaat cactgtgctg acagtatcga ccttatcatg caagcctgtg
     4261 ttaatgacag taaagagata gtcactttga gccttatccg tagcaatttt acccttccac
     4321 tcgttgagca agttactttt aacaaagttt cctacttggt tgaccttggc aaaacgcgtt
     4381 tccatctcgc ggtgaacttc gtccacgcta cagccacaga tactatcatg tggcgcattc
     4441 tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt ggttgtgtcc accagtgata
     4501 atagtcaagg gttctaccac ttgctctagg aggttgctat tttcttggaa ggcttgtttt
     4561 aggtaaatgc gggatgaaga agtgttggca agtgtgtacc agccatctgt ttcctgactg
     4621 gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg caccttctac agcttgaaca
     4681 tattcatcaa aagaactatg aacaaagatt acatccggga agagttcatt tgccacacga
     4741 atggcttcgc tcagattttt ctgtacaggc tggtggtcac agccgttcat catcaaccat
     4801 tggttggtcg aagcgtaggc acgcacatct gacaattttt gtttccagaa ggtcaaggcc
     4861 tcatctttgt caactgggat ttcattccca ttactgtacc agttggcaaa gagaatacct
     4921 aaaacacgac taccatccac accctgccag tacatttctg aaaactggga tgtaaaccgc
     4981 tcatcttcaa ggacttggtt gtcaaatcca atcggcttca caccacgacc aaaggctgcc
     5041 acgtgaatac ctgatttttg aaggatttga ggagcttgtc ccatatttcc aaaggtatct
     5101 ggaaagtagc caatctgggt tgatttaccc catttggcag cttcttgttg accaatcaag
     5161 gtattgcgga cattggcttc actggagatc aagtagtcat cctgcaagat gtaaaaggga
     5221 ccaattttaa gtttgccctc gtcaatgtag cgttggacct tgtcgcgatt ttcagggcga
     5281 atttgtaagt agtcatcaag gacaatagtt tgtccatcca agtggaaact cttgaactca
     5341 gggtcatttt caaagagatc aaagagattg tcaaacaatt ccaccaactg catacgatgg
     5401 ctttcaaaag gcaagtacca ctcacgatcc cagtgactat gtgagataat atgtacaaca
     5461 acattttcca tgaagtaaaa cctcattcta aatttaaatt ttaacgtttt aaatgtaaac
     5521 ttgtgattct gataagaatc ggttgagcta aagcgagccc cttagcggat atccaagtaa
     5581 tccaagacca actcacagaa catcatgtta gcccaggaga accattcacg agagtagagg
     5641 gtcggatcat ctacgtggaa gctttcgtgc atgacacctg taccaccatc gcaggcaacc
     5701 agctgatcca gcaagaattt tttctctgcc ttatctcttg ttgtcaatcc ttggatagaa
     5761 agggcgattg gccagatata gcgatagaag gtatgagaac ttccgagacc gctagcgtat
     5821 tctccttggt agaagtatgg attttcagag ctcagaatgg tacggcgagt ggcttgatac
     5881 acttcatcat cgatcgaaca gtagcccaga tagggcgcag ctagtagact tggtacattt
     5941 ggatcatcca tgatgctggc atttcctagg ccatctactt caaaagcgta aatcttttcg
     6001 cccttgctgt tggtggtgta agcgtagttt tcgattcctt cttggatttc atcctgaaga
     6061 cgcttggcat cagcaataac actctggcta tcagctaggt ttaatgctgc gaagatttct
     6121 tgcacataac ccaagactac tacagcaaac atatttgacg gaatcaagta actatactgg
     6181 caacagtcat cactcggacg aaaagctgac caagtcatac ctgtcactgc aaagtcaggt
     6241 ccaaagccat catttaccaa ggtgtcttcc ttacggtccg tatctcggac aaaacgataa
     6301 ggagagttct tgtggtcttg ttcaactgtc catagatgga gaatttcctt agtcgctgcg
     6361 acaaaagttt catcaaactg actagtctcg ccagtctctt tccagaggag ataagccaac
     6421 tgcaaaggat agcaaagcga atccacctca tacttgcgct cccagatcca gccgttaagg
     6481 tctgtgtggt cagtctcgtg gtgccctttc cagttctcct caatgttgaa ggagttagca
     6541 tagggatcct tgagtaccaa ggtcatctga cgtttgacca aacctgcaat ggtctgacgc
     6601 aggagggcat ctcttttagc tacatgaagg tagggtctga gttgggctgt cgaatctcga
     6661 agccacatgg caggaatatc cccagtcaag acaaaagttg aaccatcttc taagatttca
     6721 accgtattgt ccaaggtatc ggtgtagcaa cgctcaaaga catcaaccca ctctggatgg
     6781 tccttggccc gctctgctac ttcatctagc cattctctaa caatttcttt cgaataaatc
     6841 atcgtgcaat tccctctttc aaacaagttt catgttcagt atatagcttt tgagacagaa
     6901 aatacataca caaatgatag tcatttttct acaaaatagt tatctttaaa actccttttc
     6961 taaaggccct ctttttctga tataatgtag aaaaacagct aaaggaaaga ctatgaaacc
     7021 actacttgaa accatcgata cccgctttgg tactgctagc aagcatgcct tctctcgggg
     7081 aaataccctg ccatacacag gcgtgccttt tgggatgaat tactttgtgc cccagaccag
     7141 tgaccaggat ggatcttggt tttttgatcc gcatctgcct atctttcagg ggattcgatt
     7201 aactcaccag cccagtcctt ggattgggga ctactcttgg ctccttctga cacctgtcac
     7261 cagtcaactg gggggagaca gcctctttca ccgccagtct tcttatgata tagataaagc
     7321 ttgcttccag cctcactatt tgaagctctt ttctctgcgt tatcagattg aaacccagct
     7381 cacaccgact tgctatggtg cttctattcg tttgaatcaa aagcaaggca aagccctctc
     7441 cctctatctt cacgcagcag atgaactgac agtagagcaa gtagataagc ggactcttgc
     7501 cctgcgacaa gaaggtaaaa ctgaaactaa caaaaattcg ctaacgatgt tcactgccct
     7561 gcaaatgaac acggatattc ttgctatcag ccaagaagct ggggactggc gaattgactt
     7621 agcaagtagt caaaccgaga tgcaactagc cacttctttc atctctcctt ctcaagctct
     7681 aatcaatcta cctcaagaag attttgatag ctgtaaaaca agtgcccaag cggattggga
     7741 aaatctcctc catcgttttg acattataga gataggagag gctgaccgaa ccttctttga
     7801 ccactgcctc tacagactct tcctcttccc acagactttt tatgagatta atgaatcagg
     7861 gcaagccatc cacatggatc tggctactgg tactgtcaag cctggtgtcc tctttagcaa
     7921 caatggtttc tgggatacct tccgcaccac cttccccctc tttgccctta tcataccgga
     7981 gcactatcaa cgctttttag aaggtttcct caatagctac cgcgatactg gtttccttcc
     8041 aaaatggctg gctccagatg aacggggtat gatgccaggt acacttttag acggtattat
     8101 cgcagatagc gcctgcaagg acatggcccc tgacctagaa ggagaactct tccaagccat
     8161 gctcgaaaca gccagcaaag ccgaccctct cggcatcaat ggccgccacg gactagccca
     8221 ataccaagaa ctaggttacc tctctaccga ccaccacgaa agtgttagcc atactctaga
     8281 ctatgcctat agtgactttt gtatcgccag ctgtgccaaa aaactagaga acatagaaat
     8341 cgctgaaacc tacaaggcta cttcacaaaa ttaccgccag ctatttgaca ctgagacagg
     8401 ttacatgcga gcacgagata atcaaggaaa ctttcaccct gacttctctc cttatagttg
     8461 ggggcgagac tacgctgaat gctctgccat tcaagctact ttaggcgttc tccacgacat
     8521 ccctggctta atccaactga tgggtggaaa agaaaccttt agcaactatc ttttgaaagt
     8581 ctgtcaagat gctcccctct ttgaaacaac aggctatggt tacgaaattc acgaaatgag
     8641 cgagatggct actgctcctt ttggacaaat agccatttcc aaccagccta gtttccacat
     8701 tccttatctc ttccgctaca gcgattaccc tgactacact gcccttctta tcaagacgct
     8761 ccgtcagaaa gcttttcacc caagttggga agcctatcct ggagatgaag acaatggtag
     8821 tctctctgct tggtacatct ggtcagctct cggattttat ccaacctgtc caggaaaacc
     8881 aagctatgac ctcggaatcc ctctctttga ccatctacga gtctacctag ctaaagaaga
     8941 taaatggctg gatatccata ctaaacaaaa ccacagccat tttaactttg tcaaagaatg
     9001 ccgactggac aaaacactcg tatcaactat tcaacaccaa gacctcctaa aagctgaaca
     9061 actaactttc accctcagct ggctaccaaa tcactaataa ccaaaagaac ctttgcatcg
     9121 cgcaaaggtt cttcttttat gaaacttgga cttgaagttg atccacaagc tgaccatcca
     9181 ccgttaggtt cagatagaaa tccaaggttt tatctcctgc gaaatacagg gttggtagat
     9241 gcaaatcttt tttgctttcc cctgcttgga attgaaggac ttgaatctca tcctgatagg
     9301 cgacaccatg gacacctgtc cccggttgaa tcgaaatctt agcctctaaa gaggagctac
     9361 tttcactacg ttcaatccta aaatgaagag tctctccctt tgccacagct aggctttttt
     9421 ctgcaaatgc tagttcctga acaacttctt tttttgataa cccaggagtt ttataaaggg
     9481 aaatcttggt cagtagaggc atagattgtg cttcggtaat catcacgcgc accttctgtg
     9541 cctctactag cggacctcgc aagaggcgct tatgaccaac tgtgaagccc ctgccaaatt
     9601 cctgccagac accatccacc tctacttgca catggaaagc agcgattcgt tgccctagct
     9661 tcaaatcttc tcttaactca attacatcaa aagttttagg agaacctaag tcgagttcta
     9721 actggatggg caagtctgca tcgcttgccc aagagctggt ctcaaggccg tctgtcaaat
     9781 ggcgacaagc aaagtctgcg gaaagagctg gaccagatac ctcagctccc agagccaaat
     9841 cttctttata gagctcattg cgataggtcg caaattcata aagtcgttca atatcctttg
     9901 catcaaagag cccagcttga ttcggcggaa tattaagcaa gagtggagtt cctcgcccta
     9961 ctgagtgaaa gtagatttcg accaactcct cgagagactt aggatcctga tcctcatggt
    10021 agaaccagcc tggacggatg gaaacatctg cctctccgat tgaaaaaatc gtgcccgagg
    10081 gatccccgtg ctgaagatag ttcagctctg cttctgttcc tagtttatca ggattcacct
    10141 tttgccacag tggatcacct gcataccctc gttcattgcc aatccagcgg atactggtgc
    10201 cttctgttga aaaaatcaag caagcgccct gcaggtcacg aatggtttca aaccattttt
    10261 caaattcata attaaccttt tgcgcgccct ctcctctggc accatccatc caaacctcag
    10321 cgaacttacc agcattccca tagttaggat ttgataagat ttccttcaac tgagccagat
    10381 aataggcatt gtagtccgct tctcggtcca catgatagag gggactatgg gcatcccacg
    10441 gtgacaggta gacccccata tccatatcaa actctgtggc agcttgggat acttcaagga
    10501 gcaagtcgcc ctttcctctc ctccaaggac tgaccttaac cgaataatct gtgtgagctg
    10561 tcggataaag gacaaagcca tcgtggtgct tgaccaccaa aatcaacttt ttgaagcccg
    10621 tttccttgag cacacgaacc cactcacgcg catccaactg actcgggtta aagcgctcag
    10681 gatcctcctg tccagtcccc cattcttggt cataaaaggt attaggaccg aagtggataa
    10741 aagctgctag ttcatctccc agataagcta gctgagtctg acttggtaac ggtccatgcg
    10801 gtttgatttt cttcatcgtt atatatctcc ttctttagca acttctcttg ttcacaaggt
    10861 tatccacagg ttgtggataa taaaccatga tctaacagcc agctctttcc aactccaact
    10921 ggtaggctca ggatttttct cctcaatatt ccctcttcaa acagctctgt catatggttc
    10981 tgctaacacc tctcagaatg ctactctcca taattattct caggcttctt tcaggtcatt
    11041 gtgtgaagac cattcactct ccagactttt ccacagattg tgaataagtg attattctaa
    11101 actatcacct cgtcgactat aacaaaaaat aaaaagaggg tcaataagat aattatctct
    11161 tcctaaatac tcagaaaaga ttctataatt tgtacaattg aacaagaaat gcttctccaa
    11221 aatctatctt caaaagaatg ttgcatcttt ttatcctttc acacaaacat ttctctttat
    11281 acagatccaa gtgataacca gatgataatt ggacactttt tggttatttg tgtatttttt
    11341 gaaaaatttt ttaaaaaaat tttatatcct ctttaataga agtttatgca tttgtatata
    11401 tgtattccat cctatgttgt gtataataac ataaaattgt gctaaatatc ataatttata
    11461 ttattttccg agaaattata gccattgaca ttacctagga attgcgttat aacatacatg
    11521 taagcggtac ccaaaatggt gccaagtcaa aatttttaag gaggaaaata catgtcttca
    11581 catccaattc aggtcttctc agaaattggg aaactgaaaa aagttatgtt gcaccgtcca
    11641 ggcaaggagt tagaaaactt gttgccggac tatcttgaaa ggcttctttt tgatgatatt
    11701 cctttcttgg aagatgctca aaaagaacat gatgcatttg cccaagctct tcgcgatgaa
    11761 ggaattgagg ttctctacct agaacaactc gctgctgaat cattgacctc tccagaaatc
    11821 cgcgatcaat ttatcgagga atacttagac gaagccaaca tccgtgatcg tcaaaccaag
    11881 gttgctattc gtgaattgct tcacggcatc aaggacaacc aagaattggt tgaaaaaaca
    11941 atggctggga ttcaaaaagt tgaattgcca gaaattcctg acgaagctaa agatctaact
    12001 gacttagttg aatcagatta tccatttgca attgacccga tgccaaacct ctatttcact
    12061 cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta accacatgtt tgcagacact
    12121 cgtaaccgtg aaacactcta cggtaagtat atcttcaaat accacccaat ctatggcgga
    12181 aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta tcgaaggtgg agacgagcta
    12241 gttctttcta aagacgtcct tgcagtaggt atctctcaac gtacagacgc agcttctatc
    12301 gaaaaacttt tggtcaacat cttcaagaaa aatgttggct tcaagaaagt tttggccttt
    12361 gaatttgcta acaaccgtaa attcatgcac ttggatactg tcttcactat ggtagactat
    12421 gacaagttca ctattcaccc agaaatcgaa ggcgaccttc acgtttactc agttacttac
    12481 gaaaacgaaa aacttaaaat cgttgaagag aaaggtgact tagctgaact tcttgctcaa
    12541 aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg gtggcaatat cgtagcagct
    12601 gcgcgtgaac aatggaacga cggttctaac actttgacca tcgcacctgg tgtggtagtt
    12661 gtttatgacc gcaataccgt gaccaataag attttggaag aatacgggct tcgcttgatt
    12721 aagattcgcg gaagtgaatt ggttcggggc cgtggtggac ctcgttgtat gtctatgcca
    12781 tttgaacgtg aagaagtgta atcgctgttc ggcattcgtc aatagaaaat gtaaaaaata
    12841 gaaagaggaa ataataaaat gacaaattca gtattccaag gacgcagctt cttagcagaa
    12901 aaagacttta cccgtgcaga gttagaatac cttattggtc tttcagctca cttgaaagat
    12961 ttgaaaaaac gcaatattca acaccactac cttgctggca agaatatcgc tctcctattt
    13021 gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg cggctatcga ccttggtgct
    13081 cacccagaat acctcggagc aaatgatatt cagttgggta aaaaagaatc tactgaagat
    13141 actgctaaag tattgggacg tatgtttgac gggattgaat tccgcggatt cagccaacgt
    13201 atggttgaag aattggcaga attctcaggc gttccagtat ggaacggtct aactgacgaa
    13261 tggcacccaa ctcaaatgct cgctgactac ttgactgttc aagaaaactt cggtcgcttg
    13321 gaaggcttga cattggtata ctgtggtgat ggacgtaaca acgttgccaa cagcttgctc
    13381 gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct caccaaaaga actcttccca
    13441 gaaaaagaaa tcgttgaatt ggcagaagga tttgctaaag aaagtggcgc acatgttctc
    13501 atcactgaag atgctgatga agcagttaaa gatgcagacg ttctttacac agacgtttgg
    13561 gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag ctcttcttaa accttaccaa
    13621 gtcaatatgg acttagttaa aaaagcaggc aatgaaaact tgatcttcct acactgcttg
    13681 ccagcattcc acgatactca cactgtttat ggtaaagacg ttgctgaaaa atttggtgta
    13741 gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt acgctcgcca cttcgatcaa
    13801 gcagaaaacc gtatgcacac tatcaaagct gttatggctg ctacacttgg taacctttat
    13861 attcctaaag tataatttta gataataaac cgtctaccaa cagctatgag ggctgcgact
    13921 aatagcttta gtccggtcct cttttatgta atggtaatct attatttctt ataaaatatg
    13981 tgaaaaatca ttaaattgaa atctaaacgc attctattga gtgtgataaa ggagaattta
    14041 tggcaaatcg taaaattgta gtagctttgg gaggaaatgc gattctttct tctgacccat
    14101 cagcaaaggc tcaacaagaa gctttagttg aaacagctaa gcatcttgta aaattgataa
    14161 aaaatggaga tgatctgatt atcactcacg gtaatggacc tcaagttggg aatctcttgc
    14221 tccaacattt ggcatcagac tctgaaaaga accctgcctt cccactcgac tcacttgtcg
    14281 ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc tttgcaaaat gctctcttgg
    14341 atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca agttgtcgta gataaaaatg
    14401 atccagcttt tgttaacttg agtaaaccaa tcggtccttt ctattcagaa gaagaagcaa
    14461 aagcagaagc cgaaaagagc ggagcgactt tcaaggaaga tgctggccgt ggctggcgta
    14521 aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat tgaaaccatc cgtactcttt
    14581 taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg tattcccgtc gtcaaagaaa
    14641 acaatggaca tttgactggt gtcgaagcgg ttattgataa agacttcgct tcccaacgtt
    14701 tggcagaatt ggttgatgca gacctcttca tcgttttgac aggtgtagat tatgtatttg
    14761 ttaactacaa caagccaaac caggaaaaat tggaacatgt gaatgttgcc cagctagaag
    14821 aatatatcaa acaagatcag tttgcaccag gtagcatgct tccaaaagta gaagcagcta
    14881 tcgcttttgt caatggtcgt ccagaaggaa aagcagttat tacttccctt gaaaatctag
    14941 gcgccttgat tgaatctgaa agcggaacaa ttattgaaaa aggataagtt gttttactaa
    15001 taagatgtat tctatttcta gtatctttat atcaaattag aaattattct tgaaaacatg
    15061 tacaatattt caaaagatac tagttttaga ctttaatatg gtaaaacaaa tataaataga
    15121 aagcgttttc ttaaatgttt atttaagaaa gtagttggtt ttttacactt tgttagacat
    15181 caggaggaaa aacaaatgag tgaaaaagct aaaaaagggt ttaagatgcc ttcatcttac
    15241 accgtattat tgataatcat tgctattatg gcagtgctaa cttggtttat ccctgcgggg
    15301 gcctttatag aaggtattta cgagactcag cctcaaaatc cacaagggat ttgggatgtc
    15361 ctcatggcac cgattcgggc tatgctaggt actcatccag aggaaggttc gctcattaaa
    15421 gaaacgagcg cagcgattga tgtagccttc ttcatcctta tggttggtgg tttccttggc
    15481 attgtcaaca aaactggtgc tcttgacgta gggattgcct ctatcgtgaa gaagtataag
    15541 ggccgcgaaa aaatgttaat tttggtactg atgcctttgt ttgccctcgg tggtacaact
    15601 tatggtatgg gtgaagaaac aatggccttc tatccactcc ttgtgccagt tatgatggcc
    15661 gttggttttg atagcctgac tggtgttgca attattttgc tcggttctca aatcggctgt
    15721 ttggcatcta ctctgaatcc atttgcgaca ggtattgctt cagcgactgc gggagttggt
    15781 acaggggacg gtatcgtact tcgtctgatc ttctgggtta ccttgactgc tcttagtact
    15841 tggtttgttt accgttatgc ggataagatt caaaaagatc cgactaagtc actggtttat
    15901 agtactcgca aagaagattt gaaacacttt aacgtagaag aatcttcatc tgtagaatct
    15961 acacttagca gcaaacaaaa atcagttctc ttcttatttg tgttgacatt catcttgatg
    16021 gtattgagct tcattccatg gacagacctt ggcgttacca tttttgatga ctttaatgct
    16081 tggttgactg gtcttccagt tattggtaat attgtcggtt catctacttc tgcactaggt
    16141 acttggtact tcccagaagg cgcaatgctc tttgccttta tgggtatcct gattggtgtt
    16201 atttatggtc ttaaagaaga taagattatc tcttccttca tgaatggtgc tgctgacttg
    16261 ctcagtgttg ccttgatcgt agcgattgct cgtggtattc aagttatcat gaacgacggt
    16321 atgattaccg atacaatcct caactggggt aaagaaggct tgagcggtct atcttcacaa
    16381 gtctttatcg ttgtaactta tatcttctat ctacctatgt cattcttgat cccatcttca
    16441 tctggtcttg ccagcgcaac tatgggtatc atggctccac ttggagaatt tgtaaatgtc
    16501 cgtcctagct tgattatcac tgcttaccaa tctgcttcag gtgtcttgaa cttgattgca
    16561 ccaacatctg gtattgtgat gggagctctt gcacttggac gtatcaacat tggtacttgg
    16621 tggaaattca tgggcaaact cgtagtcgct attattgtag tgaccatcgc ccttcttctc
    16681 cttggaacct tccttccatt cctataaaat agtgagtgag gtgattccat gaaaatagat
    16741 ataacaaatc aagttaaaga tgaatttctt atatcattaa aaaccttgat ttcctatcct
    16801 tcagtactca atgaaggaga aaatggaaca ccttttggac aagcaatcca agatgtccta
    16861 gaaaaaactt tagagatttg tcgagacata ggtttcacta cctatcttga ccctaaaggt
    16921 tattacggat atgcagaaat cggtcaggga gcagagcttc tggccattct ctgtcatttg
    16981 gatgttgttc catcaggtga tgaagcagat tggcagacac cgccatttga agcaactatc
    17041 aaagacggct gggtattcgg acgtggtgtc caagatgata aaggcccttc gctcgcagct
    17101 ctctatgcag taaaaagctt gctggaccaa ggtattcagt tcaaaaagtg cgtacgcttt
    17161 atctttggta ccgatgagga aaccctctgg cgctgcatgg cacgctacaa taccatcgaa
    17221 gaacaggcca gtatgggctt tgcacctgac tcatcttttc ctctgaccta tgctgaaaaa
    17281 gggcttctac aagtcaaact tcatggccct ggatcagatc aactagagct tgaagtagga
    17341 ggcgccttta acgttgtacc agacaaggcc aactaccaag gtcccctcta tgaacaggtt
    17401 tgtaacgatc tcaaagaagc tggttatgat taccaatcca ctgaacaaac cgtaacggtt
    17461 ctcggagtgc caaagcatgc taaggatgct agtcaaggta tcaatgctgt catccgacta
    17521 gctaccattc ttgctcctct ccaagaacac cctgctctca gctttcttgc aacacaagca
    17581 ggtcaagacg gcacaggaag acaaatcttt ggtgatatag cagatgaacc ttctggtcac
    17641 ctatccttta atgtcgcagg tctcatgatc aatcatgaac gttctgaaat ccgtattgac
    17701 attcggactc ctgtcttagc tgataaggaa gaactagtag agttgcttac aagatgtgca
    17761 caaaactacc aactccgcta cgaagagttt gactatctag cgcctctata cgtcgcaaaa
    17821 gacagtaaac tcgttagcac actgatgcaa atctaccaag aaaagactgg cgataacagt
    17881 cctgctattt catccggtgg tgccactttt gctcgcacca tgccaaattg tgtagccttc
    17941 ggcgccttat tcccaggagc gaagcagaca gaacatcagg caaatgaatg tgccgttcta
    18001 gaagatttgt accgtgctat ggatatttat gccgaagccg tctatcgact tgcaacttaa
    18061 tcaggcaact gtttctacca aagaaaatcg accgattaat gaactgcacc ccaaaagtta
    18121 gacagaataa atctaacttt tggggtgttt tattatgaaa ttgagttatg aagataaagt
    18181 tcagatctat gaactaagaa agcaaggaca aagcttcaaa cagctttcaa aaagatttgt
    18241 gtgggtgttt cgataaactc aaacttttta gccgtggtat tgcctgattt tatagtatat
    18301 tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat ttgactgtcc
    18361 tga
//
LOCUS       DAVBCR010000038        17251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCR010000038 DAVBCR010000000
VERSION     DAVBCR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.038"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ508_001226"
     CDS             complement(<1..692)
                     /locus_tag="VQ508_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2490230.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ508_001227"
     CDS             complement(803..1057)
                     /locus_tag="VQ508_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490231.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ508_001228"
     CDS             complement(1415..1948)
                     /locus_tag="VQ508_001228"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2490232.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2150)
                     /locus_tag="VQ508_001229"
     CDS             complement(1935..2150)
                     /locus_tag="VQ508_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490233.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(2117..2260)
                     /locus_tag="VQ508_001230"
     CDS             complement(2117..2260)
                     /locus_tag="VQ508_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2490234.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            2494..4212
                     /locus_tag="VQ508_001231"
     CDS             2494..4212
                     /locus_tag="VQ508_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2490235.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4323..4670
                     /locus_tag="VQ508_001232"
     CDS             4323..4670
                     /locus_tag="VQ508_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2490236.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ508_001233"
     CDS             complement(4949..5785)
                     /locus_tag="VQ508_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2490237.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ508_001234"
     CDS             complement(5798..6427)
                     /locus_tag="VQ508_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2490238.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ508_001235"
     CDS             complement(6437..7078)
                     /locus_tag="VQ508_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2490239.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ508_001236"
     CDS             complement(7224..8453)
                     /locus_tag="VQ508_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2490240.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ508_001237"
     CDS             complement(8443..9609)
                     /locus_tag="VQ508_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2490241.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ508_001238"
     CDS             9753..10766
                     /locus_tag="VQ508_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2490242.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ508_001239"
     CDS             complement(10811..11230)
                     /locus_tag="VQ508_001239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2490243.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ508_001240"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ508_001240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2490244.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMEF"
     gene            complement(12733..13611)
                     /locus_tag="VQ508_001241"
     CDS             complement(12733..13611)
                     /locus_tag="VQ508_001241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2490245.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ508_001242"
     CDS             complement(13627..15132)
                     /locus_tag="VQ508_001242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2490246.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQSVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ508_001243"
     CDS             complement(15147..15683)
                     /locus_tag="VQ508_001243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2490247.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ508_001244"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ508_001244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2490248.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ508_001245"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ508_001245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2490249.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ508_001246"
     CDS             complement(16942..17142)
                     /locus_tag="VQ508_001246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2490250.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5209 a         3781 c         3095 g         5166 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccat cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctggctcttg ttccttaaag ctcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcctctaaag gaatctttcg gtttttgacg
     2101 actcgctttt tcctctctat gtgtccttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttgctg cctcgacatg gctgtgcccc tctttgatgt
     2221 aatctaatgc tcgctgttta aaatcagtac tatatgccat agctttatta taatatgctt
     2281 gtttaaaact aagcaactat atcaacgttt tgttatgaga agacttactt tttttactga
     2341 aattgaattt ttcctagcct caacatcata taaccccaaa attcaatctg acctggtttg
     2401 aagagatttt cgaagcagat aaggttttac taaaactaaa aaaaatgata taatcaaact
     2461 gattaaatgt tttaaaacta aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa
     2521 tgggttgatt ttgtagagct tcctgactac cttcgtcaag atttggaaaa tatggacgaa
     2581 aaaactaagg aagatgcctt ctatacaaat cttgaatttg gtactgcagg tatgcgtggc
     2641 ttggttggtg ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc tactgaagga
     2701 ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc
     2761 tacgatagcc gtcacttctc acctgagttt gcctttgaat ctgcggcagt tcttgctaaa
     2821 cacggcatca aatcttacgt atttgaaagc cttcgtccaa ctccagaact atcatttgca
     2881 gttcgtcatc tcaactgttt cgcaggtatc atggtcacag ccagccacaa ccctgctcca
     2941 tttaacggtt acaaggttta cggtgaagac ggtggacaaa tgcctccaca cgatgcggac
     3001 gctttgacta cttatatccg tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg
     3061 gaaactgaaa aagcttctgg cttgattgaa gttatcggcg aagctgttga cgtagaatac
     3121 cttaaagagg ttaaggacgt aaacatcaac ccagccttga ttgaagaatt tggtaaagac
     3181 atgaagattg tctacacacc acttcatggt actggtgaaa tgttggctcg tcgtgctctt
     3241 gcccaagcag gatttgactc tgttcaagtt gttgaagcgc aagcaactgc tgacccagac
     3301 ttctcaactg taacatctcc aaacccagaa agccaagcag cctttgccct tgctgaagaa
     3361 cttggtcgtc aagttggtgc agatgttctt gtcgcaactg acccagacgc tgaccgtgtt
     3421 ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa ccaaatcggt
     3481 gctatcatgg ctaaatacat cttggaagct cacaaaaacg ctggaactct tcctgaaaat
     3541 gccgctctct gcaaatctat cgtttcaact gacttggtaa cgaagattgc tgaaagctac
     3601 ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa
     3661 ttcgaagaaa aacacaacca cacttacatg atgggatttg aagaaagctt cggttacttg
     3721 attaaaccat ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt tgctgaactt
     3781 gctgcctact accgttctcg tggtttgaca cttgctgacg gtatcgaaga aatctataaa
     3841 gaatatggct actacgcaga aaaaacaatc tctgttactc tttcaggtgt cgatggtgct
     3901 gaacaaatca aagcgattat ggctaaattc cgcaacaatg ctccaacaga atggaacgca
     3961 acagctatca ctgtcgtaga agacttcaag gcacaaactg ctactgttgc tgacggtact
     4021 gttacaaact tgacaactcc tccaagtgat gtgttgaaat acacacttgc tgacggttca
     4081 tggattgccg ttcgcccttc aggtacagaa ccaaaaatca agttctacat tgcagttgta
     4141 ggggaaacca acgaagaatc acaagctaag attgctaaca tcgaagcaga aatcaatgca
     4201 tttgtaaaat aaagacctat aaaagatgag accaaccaac ctcatctttt ttcgtatcaa
     4261 atctaagcaa ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc
     4321 tcatggaaca atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc
     4381 aagaagctct tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt
     4441 actgccgtaa atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt
     4501 acttcatcaa tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgtt
     4561 atggaatccc aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt
     4621 gcgactcttc aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct
     4681 aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg
     4741 ttttattgtt cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa
     4801 gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg gctattccat attcaattat
     4861 ccagattcaa tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaag ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa gacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag atctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaacggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata ggatggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagcc ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatgtcttta atttgcttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg tacgtatgca ggttatttca actttaccag
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cccccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacgccaccg tttactgcaa
    11041 tccagttcac gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat aggtcacctc ttaaaattcc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cgttgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgaccc
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 gataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctaacttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gacactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgatagttca cgataagcta ccgattgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcaacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccatga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcaaatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt t
//
LOCUS       DAVBCR010000039        16998 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCR010000039 DAVBCR010000000
VERSION     DAVBCR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.039"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            470..1591
                     /gene="mnmA"
                     /locus_tag="VQ508_001247"
     CDS             470..1591
                     /gene="mnmA"
                     /locus_tag="VQ508_001247"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2490251.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1732..2187
                     /locus_tag="VQ508_001248"
     CDS             1732..2187
                     /locus_tag="VQ508_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2490252.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2197..4110
                     /gene="mnmG"
                     /locus_tag="VQ508_001249"
     CDS             2197..4110
                     /gene="mnmG"
                     /locus_tag="VQ508_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2490253.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4534..6213)
                     /locus_tag="VQ508_001250"
     CDS             complement(4534..6213)
                     /locus_tag="VQ508_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2490254.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6215..6448)
                     /locus_tag="VQ508_001251"
     CDS             complement(6215..6448)
                     /locus_tag="VQ508_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2490255.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6830..7051
                     /locus_tag="VQ508_001252"
     CDS             6830..7051
                     /locus_tag="VQ508_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490256.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQ508_001253"
     CDS             complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQ508_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2490257.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQ508_001254"
     CDS             complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQ508_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2490258.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7781..8464
                     /gene="tsaB"
                     /locus_tag="VQ508_001255"
     CDS             7781..8464
                     /gene="tsaB"
                     /locus_tag="VQ508_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2490259.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8461..8898
                     /gene="rimI"
                     /locus_tag="VQ508_001256"
     CDS             8461..8898
                     /gene="rimI"
                     /locus_tag="VQ508_001256"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2490260.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8888..9898
                     /gene="tsaD"
                     /locus_tag="VQ508_001257"
     CDS             8888..9898
                     /gene="tsaD"
                     /locus_tag="VQ508_001257"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2490261.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9944..11244)
                     /locus_tag="VQ508_001258"
                     /pseudo
     CDS             complement(9944..11244)
                     /locus_tag="VQ508_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11520..11871)
                     /locus_tag="VQ508_001259"
                     /pseudo
     CDS             complement(11520..11871)
                     /locus_tag="VQ508_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11978..12270)
                     /locus_tag="VQ508_001260"
                     /pseudo
     CDS             complement(11978..12270)
                     /locus_tag="VQ508_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12513..13719
                     /locus_tag="VQ508_001261"
                     /pseudo
     CDS             12513..13719
                     /locus_tag="VQ508_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13712..14360
                     /locus_tag="VQ508_001262"
                     /pseudo
     CDS             13712..14360
                     /locus_tag="VQ508_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14370..15987
                     /locus_tag="VQ508_001263"
                     /pseudo
     CDS             14370..15987
                     /locus_tag="VQ508_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            16007..16851
                     /locus_tag="VQ508_001264"
                     /pseudo
     CDS             16007..16851
                     /locus_tag="VQ508_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5188 a         2939 c         3743 g         5128 t
ORIGIN      
        1 ttaggtgccc ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa
       61 tgggtttaag ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac
      121 aaagataagg ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat
      181 ttcaggaaat gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga
      241 aaatgggttg ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac
      301 atcattcaca taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc
      361 ttgtcttctc caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa
      421 gcacacacga ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa
      481 ctctaaaaca cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct
      541 tttgctcaag gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac
      601 agatgaaaac ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga
      661 ccagattggc attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt
      721 tgagtatttc ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa
      781 caaggaaatc aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt
      841 agcgactggg cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg
      901 tggcgtggac aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact
      961 ccaaaaaacc atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga
     1021 agaagcaggc ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga
     1081 aaagaacttt aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac
     1141 tgtggatggt cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg
     1201 tggcggactc ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg
     1261 aaaagatcta agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat
     1321 gtcaactagc ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac
     1381 gctagaatgt acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt
     1441 caaaggagac aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca
     1501 ggcagttgtc ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta
     1561 ccgcgatgga caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat
     1621 aataacagca atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc
     1681 acttctttgt agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa
     1741 caagactttc ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt
     1801 gtccaaaatc acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt
     1861 gcgattcaag tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg
     1921 gatgtcaaag ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac
     1981 ggcgtttcct atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg
     2041 ttgaccatgc aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc
     2101 cagaatatcc agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa
     2161 ctaagacaca ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa
     2221 tacgatatta ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc
     2281 cgtatgggct gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct
     2341 tgtaatccct ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc
     2401 ggtggcgaga tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca
     2461 gggaagggac cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag
     2521 gaaatgcgca agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat
     2581 gagattttgg tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat
     2641 gctgctaagg ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga
     2701 gacctcaagt actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat
     2761 ctcaaggaac tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag
     2821 gcttcttcta tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat
     2881 ttctcataca cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc
     2941 tataccaatg gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt
     3001 acaggtgtgg tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg
     3061 cgctttgcgg acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag
     3121 gaagtctatg ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt
     3181 cattccatca aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat
     3241 gatatggtct tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc
     3301 ttcactgctg gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt
     3361 atcgcgggta tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga
     3421 agtgacggtt atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct
     3481 taccgtctct tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat
     3541 atgcgcttga ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt
     3601 tttgaaatca agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc
     3661 aagccagtca aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat
     3721 gcggtgacag ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc
     3781 ttcatcggac cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa
     3841 atcaagtacg aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg
     3901 gaagaaaaac gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa
     3961 gctcgtcaga agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg
     4021 ggagtaaacc cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt
     4081 atttctaaaa ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca
     4141 aagcttggct ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc
     4201 aagttatgca ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa
     4261 agcatagaga aaaacagctc cgaagacggg gctgttttgt tgacttgtac tcaatgaaaa
     4321 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactgcttt gaggttgtag
     4381 atagaactga cgaagtcagc tcaaaacaca gttttgaggt tgtagataga actgacgaag
     4441 tcagctcaaa acacagtttt gaggttgtgg atagaactga cgaagtcagt aacatatata
     4501 cggcaaggcg aagctgacgt ggtttgaaga gtattattct tcatctggtg tgaggatcat
     4561 cgggatgatg atcggttcac gttcggtatt ttcatagagg aaggggcgaa tagcgttgac
     4621 aatggcacca ttgacagatt gcacgctagc atccttattt ttcagtgcga tacgaatggc
     4681 attgaagagg atacgctggc tttggcgaat caagtctcca gactctctca tgtagacaaa
     4741 gcctcggctg aggatatctg ggccagacag aatcatctgc gatttgaagt caacagttgc
     4801 gactgccaga acgacaccgt cttcagatag atcgcgacga tctttgagga cagctgcgcc
     4861 aatttcaccg atacgatttc catcgacata gatatcttgg gcgttgaaat gacctgcgat
     4921 acgagctgag tcagcagtaa gggcaagcac atcgccattg ctcatgataa agatattgtc
     4981 tttctcaaca ccagtatcca ctgctagtcc agcgtggact ttttgcatgc ggtattcacc
     5041 gtggacaggc atgaagtatt ttggcttaat caagcggagc atgagttttt gctcttgctg
     5101 accaccgtgt ccagatgtat ggatattgtt cactttaccg tggataactt cgacaccagc
     5161 ttcagaaatg atgttaatca gcttgttgac gctagtagtg tttccaggga tgggactaga
     5221 agagaagata acggtatcac ctggttgtaa ttgtacttga cggtgggttc cgttggcgat
     5281 acgagagagg gctgccatag gctcaccctg actacctgta cagaggataa gaacttctcc
     5341 tgcaggataa tctttgattt catttggctc gataaaggtt cccttaggag ctttgatgta
     5401 gccaagatcg attccgttga caatggcctt ttccatagaa cgaccaaaga ccgcaatctt
     5461 gcgtccagtc ttaacagcag cttctgttgc ctgctggaga cggaagatat ttgaggcaaa
     5521 ggatgcaaag atgatacgtc cttcaatacc ttggataatc ttcataatgg actgaccaac
     5581 gactttttca gagttggtaa aggttggtac ttccgcattt gtcgagtcag acaggagaca
     5641 gagcacgcct tcttcaccaa gcgcagccat acgatgcaag tccgcaggtt ctccaactgg
     5701 agtaaagtcg aacttaaagt cacccgtaca gacgattttc ccttgaggag tatgaatgac
     5761 aatccccaaa ggctctggaa tagagtgagt cgttctaaag aaagttgcct tgagattttt
     5821 aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg gcgttgcgca agaggccgtg
     5881 ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt ccagcgtaga tagggacatt
     5941 tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc tcgtgtccgt gtgtgattaa
     6001 aacagccttg acgcggtcga tattgtctac gatgtaagag tagtcaggaa tgacatagtc
     6061 gataccaagc aagtcatctt ctgggaattt aatcccagca tcgacgatga taatctcgtc
     6121 ttggtattca attccgtaag tgtttttccc gatttctcct agaccaccga tggcaaaaac
     6181 accgacttct tcaggtttaa gagtgtaggc catattagaa ctccgtaatt tcgaaggcgc
     6241 cagtttcttt ttcgtaatcg agcaatttgt cagacaagag ttcgatatat tcgatattgt
     6301 actctgggcg attttcttcg acaagttggc gagcagtgat acggccctca agttctgagc
     6361 tggcatcgat gtctaggtaa agcgcgcgtg ttgtttcacg gcgtgggcta cgttcttttg
     6421 tttcttgata aaaaactttg taaatcatat agttcctttc tatttacagg tcagcttatt
     6481 ccaaactttt agcagatatt tgcttgattt cattccattt tgtgtctaga ttgaccttga
     6541 ttcgttacaa ttatttcaat tataccacaa aatgaaaaat tagtaaaaga cggaaacgag
     6601 aaagttaggg gacgatttct tcggtaagcg tttgcaaaaa caaagaaaat gtgttatgtt
     6661 gataacggaa gtgaagttta ttacttgtca taattccata tagattttaa gtcaaggagg
     6721 ttgccctatg tataacttat ttgcttttgc tatcggagtt cgtttgctag cttttatcgt
     6781 ttttgttttg cgtttatgga ggaaatcttc tatttgccaa actggaacga tgacaaacca
     6841 agttcttgag gaaaattacc tttgtgactc tctactcgct ttttctcctc ctcgtgacct
     6901 atgtcgtttg gtttgtattt tactttggat taaatgctta gatgccctgc ttatgctggg
     6961 gttttttgtt tggggatgta gagatagtag atttacgaca cgaaaattcc accccatcta
     7021 tcaagatgag gtggtctttg catttttcta aaatgatatg acaatggaat tttcaaagct
     7081 tattattttt atgggagtca ttgttctgct caaaaataga gttgaagcat aaaaataaaa
     7141 caatacaaac agataaatcg aatggtaaag ggatggtagt aatgttcaaa tcattgagaa
     7201 tgctgagcaa actgtaagag atgaagacaa taaaatttat ccttctaaaa atgtgtttct
     7261 tatctagcat gacttaccaa actttttacg aaggtctttt ccaatggtat aacctgccat
     7321 aaataaagcg ataccaacac ctacaccaaa gccacccttg atttcttgca attccttatt
     7381 agaaatttca cgacagttgt tcaaatcttt catcatatta atctcctcct aaaaaataac
     7441 cagctgccaa accagaacct ataagtgctg ttccacctgc aatagctttc caagatactg
     7501 ctactccaaa gataaccaag ggagcgagac cgccatcaat atctgataat tcattttcag
     7561 ataaggataa aaattgattg tcaagaatgt caaaatttgt cattgaactt tcctcctttt
     7621 ttcttgttca cttttatatt cggaaaagaa aaggaaaagt gagcattttt ttaaattatt
     7681 tttagttaag tgagggcact cttgtctggg ggcaaagaaa attcccatgt gccatctttt
     7741 gtagtataat agtaagcaga caaaaagata ggaatgtgtt atgaaagtat tagcttttga
     7801 tacgtccagc aaggctcttt ctctcgctat tttagaggat aagcaggttc ttgccgagac
     7861 gacgattaat atcaagaaaa atcacagtat taccctcatg ccagccattg attttttgat
     7921 ggagagtttg gactggacgc ccaaggattt ggaccgaatc gtggtggcgg aagggccagg
     7981 tagctacaca ggcttgcgaa tcgcggtagc cactgccaag accttgactc atactctgaa
     8041 cattgagtta gttggtatgt ctagcctctt ggctctggtg ccatatcaac aagaaggctt
     8101 gttcgttcct ttgatggatg cgcgtcgcaa caatgtttat gcaggatttt atgaaaatgc
     8161 taaacctgtc atgccagaag cgcacctatc ttttgaagag gtgctagaaa aagtcaaggg
     8221 tgctagtcag gtaacctttg tcggagaagt tggccccttt gtggagcaga ttcaagaaca
     8281 cttgccaagg actaattata aagaaacctt gcctaatgca gccaatcttg ctcttttagc
     8341 ttgggacaag gaagcagact ccttgcatga ttttgtgcca aattacctca aacgagtcga
     8401 ggctgaggaa aactggctca agaaccatac cgagtctggt gagtcctaca ttaaacgcct
     8461 atgatagaaa tcaagcgaat tcaacaacag cctgacctag ctcaagccat ctacgctgtt
     8521 atggcagctg tttacctagt cagtccttgg actctggagc aaatccaagc agatctgtcc
     8581 caagaccaga cttggtatgc attggcttat gatggggcag aagtgattgg atttctagct
     8641 gtgcaggaga atctttttga agcagaagtc ctgcaaatcg ctgtcaaagg agcttatcag
     8701 ggtcagggaa ttgcgtcagc cttgtttgct caattgccga cagataagga gattttcctc
     8761 gaagtcagaa agtcaaatca acgagcgcaa gcattttaca agaaagaaaa aatggcggtc
     8821 atcgctgagc gaaaggccta ctaccatgac ccagtcgagg acgccattat catgaagaga
     8881 gaaatagatg aaggatagat atattttagc atttgagaca tcctgtgatg agaccagtgt
     8941 cgccgtcttg aaaaacgacg atgagctctt gtccaatgtc attgctagtc aaattgagag
     9001 tcacaaacgt tttggtggcg tagtgcccga agtagccagt cgtcaccatg tcgaggtcat
     9061 tacagcctgt atcgaggagg cattggcaga agcagggatt accgaagagg acgtgacagc
     9121 tgttgcggtt acctacggac caggcttggt cggagccttg ctagttggtt tgtcagctgc
     9181 caaggccttt gcttgggctc acggacttcc actgattcct gttaatcaca tggctgggca
     9241 cctcatggca gctcagagtg tggagccttt ggagtttccc ttgctagccc tcttggtcag
     9301 cggcggacac acagagttgg tttatgtttc ggaggcagga gattataaga ttgttgggga
     9361 aacccgtgat gatgcggttg gtgaggctta tgataaggtc ggccgtgtca tgggcttgac
     9421 ctatcctgca ggtcgtgaga ttgacgagct ggctcatcag gggcaggata tttatgattt
     9481 cccccgtgcc atgattaagg aagataatct ggagttctcc ttctcaggtt tgaaatctgc
     9541 ctttatcaat ctttatcaca atgccgagca aaagggagaa agcctgtcta cagaagattt
     9601 gtgtgcttcc ttccaagcag cagttatgga cattctcatg gcaaaaacca agaaggcttt
     9661 ggagaaatat cctgttaaaa ccctggttgt ggcaggtggt gtggcagcca ataaaggtct
     9721 cagagaacgc ctagcagccg aaatcacaga tgtcaaggtt atcatccccc ctctgcgact
     9781 ctgcggagac aatgcaggta tgattgccta tgccagcgtc agcgagtgga acaaagaaaa
     9841 cttcgcaggc tgggacctca atgccaaacc aagtcttgcc tttgatacca tggaataaag
     9901 agtggctctt tgtcaagtgt agtgggtagt cgaaaagctg caatctggag agtacgaaat
     9961 tcgtactctc ttttttgatg ttcaaagtca tcaaaatccg ttttttaaaa ttgttaaagt
    10021 tccggaaacc aaaggctttg cgcttgataa tcttaatcaa attattggtc gcttcaagtt
    10081 tagcgttgga ataaggcagc ttaagtgcgt tgataatctt atctctatcc cttaaaaaag
    10141 tccaaaagac cgtttgaaaa agcggatgaa ccgttggcag ttcttgctca atcaatccaa
    10201 agaaatggtc ggcattcttc tcttgaaaat gaaagagcag gagttgatag agttcgtagt
    10261 gaacctgtaa tccctcagag taagaaagca gcttatctcg tacttcccga tgggttaagt
    10321 gcattcgaaa cgtgggctga tagaatcgtt tctgacagag cttgaagcta tactggtgta
    10381 gtaaatcagt ccagtggact gatttagccc gagcctagaa acgaaaaagc gagggttcca
    10441 aaagcgcttg agcgcccgat actccaagga ttttcggtca aattggttca tgatttggat
    10501 tcgtactcgg ttcatagctc ggctcagatg ttggacaatg tggaagcggt caagaacaat
    10561 cttcgccttt ggaaagagtt gcttagcgag ccgataataa gggctgtaca tgtccatggt
    10621 gatgacctcg accagctccc gaacctctct ctgatagcgt tggaaatggt ttcgaatcac
    10681 cgcatgagtt cgcccgtcta aaattgcgag gatggatttg gactcaaaat cttgggcaat
    10741 gaagctcatt ttgctctttt tgaagctata ctcatcccaa ctcataactt ttggcaactt
    10801 ggtccaatcg gtttcaaact taaattccct cagttttcgg atgacggtgg aagttgagac
    10861 cgccaatctg tgggcaatct cagtcataga ctgcttttca aggaggagct gagcgatttt
    10921 ttgtctcacc atgttggaaa tctggcaatt tttcttgaca atggatgttt gagaaacgac
    10981 caccttaagg caattcttgc actgaaagcg gcgctttttg agatgcagta ccgtgggtaa
    11041 accctgacag tcgagaagcg gaattttaga ggctttctgg aagtcgtatt tgatcatctt
    11101 cccttgacaa tgaggacaag gaggagcggg ggaatccaac tttgcctgaa cgactagatg
    11161 ggtttggtat ttcagaacag acaagatttt gatatttttg tcttccaatc cgagcaaatc
    11221 tgtggtattc ttaagttgtt ccatatggac cctttctaat gagttggtta gacgcttttc
    11281 attataggtc atatggggct ttttttctac aagaaacgac cctataattc ctggggtggg
    11341 attacccact acagaaatta tagagccaaa gcattccaaa gtcttgtctg actctggaat
    11401 gttttctgtt cacagttgtt ttgtaactgc ctaataagta accgcgctat aacgaggtat
    11461 ctaccgagat tgtcaattct ccagtatact gttataaaaa acaaaactgg aggatttttt
    11521 tatgtcctcc catcgtccca tcgactgttc cgtagctgcc caataacgga gtatagtcgg
    11581 ctgaaacaag aataggacaa aagtgcctca agaaaagcat cgtaatttga caatattatt
    11641 ctgagatgtt tttgatgtaa gcccatattt tctcaatggg attatactcg ggtgaatagg
    11701 taggaagtgg taacagtcta tgccctgctc cttacacatg ttcattctgt gaaaccttgc
    11761 attgtccata atgataaggg atggtgtatc taaagtaggt agtaagaatt tttggaagca
    11821 agcttcgaaa aagtcactcg tcatcgtatc tttataggtc atcggagcca tagtatattg
    11881 aaacaaaaaa gggcattagt acttctataa cagtatcgca atctctggtg tgcgttgtct
    11941 cgatcttaat tcaaataatt atatcttctt ccagagagac taactgcctg ctcaaggctc
    12001 gatcatattt gagataaaaa taggtctcaa cccctgtctc ataaatataa ataggagtca
    12061 ggtagcttaa gtgattcaat tctttaagga accgatttac tttttctggg tcttgttcgc
    12121 agtaggtacg gctcttttta gactatatcc catagccttt agagcgtaat gaatagttgt
    12181 tggaggacag ttgaattcag cagctatttc agtcaaataa gcatcgggat gagtttcaat
    12241 atagatcttt aatttatctc tatcaacttt ttcaattaat agtggaagtt ttcttaaagt
    12301 tagtgtgttg aaacgttttt atacagtgct aaaatttgaa gtgtagtgca gatgtatttg
    12361 taatagtatt atagcaattc tttggagaag gtcgagacaa gagaaaatag gtatctttga
    12421 gatataagta tgatttacta taaaattatt gagttattca taaaaatttc tctatcaaat
    12481 atcataatgc gtcgtttaag gagatggtta caatgcaata tagttgtgga aaaataaata
    12541 ttaatatccc tgatggatat ggagacatta aggatatagt gttttcggct catattatag
    12601 ttaggtataa taatggacat tgtggaggta tagatccgca tataattgga ctttgtaaaa
    12661 agcagatcag aaggatgtct ccatatccta ttttgataat cgtatctaga gattcaaagg
    12721 ttattgatga ttataaaaat ttagatattg cctatgttga ttgtactcaa tgttcaaata
    12781 attttgaaac tgccttacac gttaaaaata ttttaaaatt attaaaaatc caacttatac
    12841 actgtcatgg ctactcaact aattattttt tatatatgtt aaaaaaactt gacaagaatg
    12901 gatttggaaa ggttaagaca gtgataacat gccatggatg ggttgaatat aacttaaaaa
    12961 agaaattctt gacttatttt gatttttgga catattctat gggagatgct tttatttgtg
    13021 tatctgaaac tatgaaaaaa agattggaga gtataataaa aaaataagaa gatagttgca
    13081 ataaataacg gaattaatgt atcaaatagc gatttagatg tagtaggtgt tcaagacttt
    13141 aaaaaagaat tttgtattcc aaataacaaa aaaatcattt gttatgttgg aaggttggat
    13201 ccagaaaaaa ggccggatag attccttgaa tttgcagaaa aattattttt agtcagagaa
    13261 gatgtgattt ttattatggc tggaaatggt agtatgtggg ctactttgaa agaaaaaatt
    13321 tgccatttga aatgtagaga taatttcagg ttacttggtg aaatctatcc agctactata
    13381 gtttaccaaa tatcggattt attgtatatt ccttctgata cagaaggaat acctatgtgt
    13441 gttttagagt caatgtcaca agaaactccg gttttagcta gtaatgttgg cgggttaagt
    13501 gaaattattg aacatagggt tgatggattt ttatttgaga aggaagatgt tgagggagtg
    13561 tgtgcttgtg ctaatttttt actcaatgat tctgagtatt tgaaatatat aggtgagaat
    13621 agtaaatcaa aaataagaaa acatttttct gtgcaaaaaa tgtttgtaga aaccatgaga
    13681 gtatatgatg aattattaga gaagagtagt catggatagg aatatagatc aagaattagt
    13741 aagtattata ataccaacac ataataggta tgaatctctg attagagctg tgaaaagttg
    13801 tttacatcaa agttataaaa atatcgaagt tataattatt gatgataatt atagtaatgt
    13861 aaatttaaga aataaaatta tccatcaatt tggctatacc agtcatagaa ttaagttaat
    13921 tttaagtaat gaagatttag gtgcaactaa tgccagaaac ataggtatca aaaattctag
    13981 aggtaagtat atatcatttt tagacgatga tgatgaatat atgccagatc gaattttaaa
    14041 gttgatggct tgttttaaaa agagtagaat gaagaattta gctttagttt atagttatgg
    14101 aataataatt tatccaatag gaaatatttt aatttattga gtttcaacga gagaatattg
    14161 gttacaaaga aatattattc tttaaacata aagcggttac tattaatagg agacaaatgc
    14221 aaggctttaa aagtaatcaa gaaggcaaga gaagaaaaaa tttttaacga atttcttttt
    14281 cgaaatatat gttattatat cattttggtt ttttctattg tatatatgac aactatgttc
    14341 aattaaaatt tagaaagtga gaaactattg tgtatactat tataaaatca aatataaaaa
    14401 aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt tatgcagcaa
    14461 ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg tttttgaaat
    14521 tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg gtagtgaaaa
    14581 attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga gttgccacag
    14641 acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca tatctttcgt
    14701 ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt tttttagtaa
    14761 taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat cattggtcat
    14821 tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa atctttgcat
    14881 cgaaaatgcg agaagttagt ctaaatttaa ctaaccaaaa tgaagctttt ttaaaatcta
    14941 gtgagactat attgaatgga tttgatgtgt tagcgtcctt gaatctttta tatgtattgc
    15001 ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga aagacaactg
    15061 tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag atatctctcg
    15121 tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact attgaagcaa
    15181 taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt caattatcct
    15241 ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca attgagtcaa
    15301 ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta tatgaagcaa
    15361 aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat ttttgttttc
    15421 aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct acattattaa
    15481 aattattgag tggctttttg agagattata gtggagaatt gcgattctgc ggggatgata
    15541 taaaaaaaaa cctcctattt aaatatggtt tcgaatgttc tatatgtaga tcaaaaagct
    15601 tatttgtttg aaggtacgat tagagataat attttattgg aagaaaatta tactgatgaa
    15661 gaaatactac agtctttaga gcaagttggt ttgagtgtaa aagattttcc taataacatt
    15721 ttagattatt atgttggtga tgatgggaga ttactgtcag gagggcagaa acaaaaaatt
    15781 actttagcta gagggctaat tagaaataag aaaatagtat taattgacga gggaacttct
    15841 gctatcgata ggagaacttc gttagcgatt gaacgtaaga tattagatag agaggatttg
    15901 actgtcatta ttgttaccca tgctccgcat ccggaactta aacaatattt tactaagata
    15961 tatcaatttc caaaggattt tatttaagaa tataggggag ggaaacatga gattctattg
    16021 tgaagcttat gaggtaatta ctgaagagaa aaaagtatac tttaatgata ttgagttaga
    16081 agttaagtat tcctctgtcg aagaattgtt tataatatgt gagaaattat tagataagaa
    16141 aaaagtatca tttttttatg tatatgagaa acccttaagg tatctgttat ttgactatat
    16201 atttctacta gttctagcta aaaaaaatat cccaatctta gatggagttt ccaataagca
    16261 agttgatcca acgttgctgc atcacttttc tttggaaatt gaaaagaatt ttatcgattt
    16321 ttgctataaa aatatggatc tcatcctaaa aactcaaagt atatctttgt gtcatagaga
    16381 agagcttatt attgtagatg tttcagatga ctctaaattt ggtgtttttt acaagcgttt
    16441 tagaacactg gttgataaaa ataacggaaa atatgtttgt gtttataatt tcagtcgtgt
    16501 ttcagatata ctgcaaaact ggaaaaatta ttgcaatcga aaattttcgg ttacgtttac
    16561 tgaaagccaa tttgaattat ttaagctttt atataatcaa aaaaatttta aaacaatttc
    16621 gttgctattt ggaaaaaaat tgttgctgga ggaattatct attatagcga tcttactaat
    16681 atagaatact tttgtatttt ttggtgggat agtatgtttg ggaaagattc tattggaaaa
    16741 tacgtatatg tagaagagat tagtagatgt cattttttag atagaaatta ttctttttgt
    16801 tatggtttac aagattataa aagtaaactt attaaatatt ttttagaata gttaatctat
    16861 tcgagagaga taaagaaata tggaaaatac tttattgaat atcacaggaa gcttcgatat
    16921 ggaaactagg aatctaatta gttattcttt aactgatatc tttgaaacag ataaaataag
    16981 aattgaactt cttggaga
//
LOCUS       DAVBCR010000040        16707 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCR010000040 DAVBCR010000000
VERSION     DAVBCR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.040"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ508_001265"
     CDS             63..527
                     /locus_tag="VQ508_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2490262.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ508_001266"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ508_001266"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2490263.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ508_001267"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ508_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2490264.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ508_001268"
     CDS             1667..2596
                     /locus_tag="VQ508_001268"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2490265.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ508_001269"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ508_001269"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2490266.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ508_001270"
     CDS             3812..4648
                     /locus_tag="VQ508_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2490267.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ508_001271"
     CDS             4684..5001
                     /locus_tag="VQ508_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2490268.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ508_001272"
     CDS             5003..6697
                     /locus_tag="VQ508_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2490269.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ508_001273"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ508_001273"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2490270.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ508_001274"
     CDS             8355..8537
                     /locus_tag="VQ508_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2490271.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ508_001275"
     CDS             complement(8815..9576)
                     /locus_tag="VQ508_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2490272.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ508_001276"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ508_001276"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2490273.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ508_001277"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ508_001277"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2490274.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ508_001278"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ508_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2490275.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ508_001279"
     CDS             13717..13980
                     /locus_tag="VQ508_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2490276.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ508_001280"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ508_001280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2490277.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..>16707
                     /gene="phtA"
                     /locus_tag="VQ508_001281"
     CDS             15974..>16707
                     /gene="phtA"
                     /locus_tag="VQ508_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2490278.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYR"
BASE COUNT         5236 a         2933 c         3475 g         5063 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
    16681 aaatctgtca aattcaagaa cctatcg
//
LOCUS       DAVBCR010000041        16656 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCR010000041 DAVBCR010000000
VERSION     DAVBCR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.041"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..425)
                     /locus_tag="VQ508_001282"
     CDS             complement(<1..425)
                     /locus_tag="VQ508_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490279.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEA"
     gene            complement(594..1907)
                     /locus_tag="VQ508_001283"
     CDS             complement(594..1907)
                     /locus_tag="VQ508_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490280.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            complement(1900..2448)
                     /locus_tag="VQ508_001284"
                     /pseudo
     CDS             complement(1900..2448)
                     /locus_tag="VQ508_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2529..2591)
                     /locus_tag="VQ508_001285"
     CDS             complement(2529..2591)
                     /locus_tag="VQ508_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2490281.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(2760..3748)
                     /locus_tag="VQ508_001286"
                     /pseudo
     CDS             complement(2760..3748)
                     /locus_tag="VQ508_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3697..4824)
                     /locus_tag="VQ508_001287"
                     /pseudo
     CDS             complement(3697..4824)
                     /locus_tag="VQ508_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(4987..5871)
                     /locus_tag="VQ508_001288"
     CDS             complement(4987..5871)
                     /locus_tag="VQ508_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2490282.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(5983..6837)
                     /locus_tag="VQ508_001289"
     CDS             complement(5983..6837)
                     /locus_tag="VQ508_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2490283.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(6895..7653)
                     /locus_tag="VQ508_001290"
     CDS             complement(6895..7653)
                     /locus_tag="VQ508_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2490284.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(7715..7903)
                     /locus_tag="VQ508_001291"
     CDS             complement(7715..7903)
                     /locus_tag="VQ508_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2490285.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(8225..8791)
                     /locus_tag="VQ508_001292"
     CDS             complement(8225..8791)
                     /locus_tag="VQ508_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490286.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(9059..10738)
                     /locus_tag="VQ508_001293"
     CDS             complement(9059..10738)
                     /locus_tag="VQ508_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2490287.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(10830..10991)
                     /locus_tag="VQ508_001294"
     CDS             complement(10830..10991)
                     /locus_tag="VQ508_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490288.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(11675..11989)
                     /locus_tag="VQ508_001295"
     CDS             complement(11675..11989)
                     /locus_tag="VQ508_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490289.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(11955..12344)
                     /locus_tag="VQ508_001296"
     CDS             complement(11955..12344)
                     /locus_tag="VQ508_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2490290.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(12732..13526)
                     /locus_tag="VQ508_001297"
     CDS             complement(12732..13526)
                     /locus_tag="VQ508_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490291.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(13526..14533)
                     /locus_tag="VQ508_001298"
     CDS             complement(13526..14533)
                     /locus_tag="VQ508_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2490292.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(14533..15540)
                     /locus_tag="VQ508_001299"
     CDS             complement(14533..15540)
                     /locus_tag="VQ508_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2490293.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(15530..16555)
                     /locus_tag="VQ508_001300"
     CDS             complement(15530..16555)
                     /locus_tag="VQ508_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2490294.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         5217 a         3142 c         2254 g         6043 t
ORIGIN      
        1 gcttcatcta aaattacaat actaggatta tttattaatg cacgtgccag tgctatccgt
       61 tgcctttgcc cacctgaaat atttgacccc atctctgaga tgatagtatt aaatttcatc
      121 ggcatagcca tgatttcatc atagatttga actgctttac aaacttcctc tatcttttgt
      181 gaagtaactt cgttctttaa agttatatta tcaagaatac ttcggttcaa taaaaatgaa
      241 tcctgtggaa ctactcctaa attttgactt aatatttttt tatctatttg attaatattt
      301 acaccatcaa ataaaatttt tcctgtatca atcttatata atcctgatag tatttttgat
      361 aacgtacttt ttcctgaccc cgattgaccg actagtgcaa ttctctgtcc tggctcaata
      421 gtcaaattta aattttctat tacaggagct gagtctttgg aataagagaa agataggtcc
      481 ttaatatcta ttctaccttc taaagaacac tttttaagac cattttcaac attctcttca
      541 ttttcaagcc agatatcatt tactctattt aagtatcctg ctgccaaaat atactacgta
      601 taagcattaa atattgagat ttctgaacta aataaaattg atgccatcgt ttgaaatgcg
      661 acaacctcac ccaataaaat ttttccattt aaaacattat caagtccaaa aatcaatata
      721 attataggag cgaacaattg aaatacatta gtagctgaat tgtataaaat atgcacaata
      781 cttcgttttt tatactttgt caatacgttt tttaaatact tagaccagtt ttcatatatt
      841 tcatcttcta atcccgatat ttttattgaa gaaatagtat ttaatgcttc tgtttctact
      901 gagtaaatca atgattgttc ttgtaattct ctattaaccg tatcatacat tatttttcta
      961 gtactaaaca gaaacaaaaa attgattaaa ctcaatataa gagctataat tgataatgtt
     1021 tttgaactaa gaaacatata tattacaaca cttagaatag tcccgatatc tactactccc
     1081 ccaacaactt ggttagtaaa tagctcccta aaaccactaa gactagaaat tctgtataaa
     1141 atatctcctt ttgccctgag ttcaaaaaaa ctatatggta gtttaagcaa atgcttaaat
     1201 gtaaaacttt caatatctct actaaaaaat atattagaaa atagaattcc catactccta
     1261 gccaagatag aaataaggta caaacaaagt agagaaaata aaattaaaaa cgaggtttga
     1321 aaagaaaatg tattccctaa tgcagaatta ataaactttg aagtcattac tggtacggaa
     1381 agtattatta agtaacttat tattgaaaat agtaacgtca aaatcaatcg accttttacc
     1441 tttgaaaaac tagagaatac tcttatccat ggagaaggaa actgcgactt caaagattga
     1501 taattttcgt taggaaatga tagcagtact atattactaa aatgttttga aaactcctta
     1561 aactcataat ttatatatcc tttagaagga tccattatat agaccttatt cttttttact
     1621 ttgtaaataa ctacataatg ttgattatcc cagaatgcta ttaatggtaa cggaagttca
     1681 ctaattttat ttacatcttt aacttgatat gagccagtat caaaatttcg tttattcaat
     1741 aactgtttca agtcacctat actataacca tctctccctt tttctatatc acgtcttaaa
     1801 tctagtaatg tctctttgct tttatagaaa tgtagtatgg acaagcagca acataaacca
     1861 cattctattt gagacatttg ctcaatatta ggtacttttc tagtcatcat gtcctcttaa
     1921 ctccagtaat aaaacattaa gtaatctatc atcttgtttt cgagatattt catagacaac
     1981 tccagatact cctagcataa aagaaaatgt attcatagta ttcaaatccg tacttaattc
     2041 tggtttgata acctgagaaa cgacattatc aaaataaaaa tctatagatt cttttattaa
     2101 taggttttct tcatctttca atattgcatc aagacaactg atagtaccta cagaaccgtg
     2161 acataaattc aattctctta ttttttctac attagttaat acattttcaa ttattgataa
     2221 atcattattc ccaatattag ttttatcgaa aatttctttt ctagctaata aaatgcccgg
     2281 agctccataa caccaaaaat taggaaagga atcattatga tttcttcata tgtctttcca
     2341 attttttaat atattattga aacatttatc ttctttatat agtaattcct ctatacattt
     2401 taatatatct gtactaggat taatttcata ataatatgat aattacattg ctactccaga
     2461 ataatcgtgt gctaaacctg ttactgttat attatctaat tgtgaaatac aataattaat
     2521 acactttttt aaatgatgat aatatccatc accaaaacca aaatcggtaa aaattttgaa
     2581 attttcttca tacttggact tcctttccgt ttttatttat tgtacaataa ataaaaacgg
     2641 atttaatttt tgttgggaaa atgggaaatt atgaaatcaa aacttggcat cacacttaga
     2701 aaagttcgta aaggaaaaca aattagtcta tgctctgtag cagatgaaca tctttcaaaa
     2761 tcacaaatat ctcgttttga acgtggggag tcagagatat cttgtattcg actaatcaat
     2821 attttagaca aattacatat tactttgtag gggagttttt ggctatcgtt gctcaattgt
     2881 ctgattaagg tcctaccctt gatgaaacaa ttattatcca tgttttcttt attatagaca
     2941 aagtaagaag acgtttctcg aatgtagact ttatattttt tatgattttc ttcttccata
     3001 atatccaatt gatagttggg aatgaaaata agaccgctct gtttgacacc gaaagacacc
     3061 ttgatataga cgcccttatc aactagcttc tctatttggt tctctgcaag ttccacttca
     3121 aattcacgaa cggtatctca tttttcctta aatgtcttaa aggcttcctc aatctcttca
     3181 gtggatactt tatccttatc tcgttcttct tggaaagcat ggtactgttc ctgtaaattc
     3241 tctaatcctt ctgaagcaac gacttcctta tttttaaaat aatcttgaaa aaatttgaca
     3301 tcatataatt tcttatcact tattttttga tgacccaaac ttatcttttg attattttct
     3361 tccaggataa aagttacatt ttttgtttta agtcaatggt tagattcaat tcttttgctt
     3421 ttgttattaa atcttctaaa gaattgacac ggtttaacaa aaattctaaa cgactttcaa
     3481 tctcttgctt agcaaaatgc gttctaaaaa attcttcatc atatagatct cgtttgctga
     3541 gttggcgccc tcgaattggt tttatcatcg ttctatctgt catcaaaaaa cggctatgct
     3601 tttgactaaa atcaatctga acatgcaact gctttgcttt ctctaaaaaa tcatcaaacg
     3661 atttagattg ctgaagcaaa aaataaagac gttgtttcaa ttcaaattta tgactagatt
     3721 ccttatattt tttataatct cgaaaagatc aggattttta gctaaaactg ccttacgaaa
     3781 atccttccag aactgatggt caatctcatt agccacatcc aaacgccaag catcgatatt
     3841 aaactcttca atccaataag tcgcaacctt taaaagataa ttcttgacct ctggattggc
     3901 tgtatttagc ttaggcatat agtcctcgaa accaaaaaca tgatagggta agtctctctt
     3961 attaactagc ttttcagttg tcaatgggaa ttgttgaata tggaaccaat ccttataagc
     4021 agactgttca ccatttttga cgacattttt tcattgaaga gattgcgaac caatatgatt
     4081 aaataccgca tccagcatga ctttcatgcc acgatgatgc gcttgatcca ccagttcccg
     4141 aaaggtctcc ttgtctccaa aataacggtc aatttcaaag taatctgtcg tattgtactt
     4201 gtgattactt gtagattcaa agatgggaca aagatatagt ccagtaatac ccaagtcttg
     4261 caagtaattc atatgatcaa taatcccctg taaatcacca ccaaagaaat catcgctctt
     4321 aggtgtgaca gatgaatccc agtctaaagt cccttctggg tttaatagag cattgccatt
     4381 ggcaaatctt tcaggaaata tctgatacca taccgtattt gaaacccagt caggaacctt
     4441 gcaggcatca atctcatgaa gataaggcaa cttaaatcca ttcccaattg catgaagatt
     4501 ttctagagaa ttttccacac accctttatc gccatacaaa atattttgac cttctgtatc
     4561 tctgagctca aagagatact ggatacgtgc aaagtcaact gacacttcaa cctgccaatg
     4621 gtcaaataag gtaccagaag ttatcttgac catttctttt gtatcctgat aaaactcttc
     4681 cataaagata aaagggtccc catagtgcaa gttgatgctt tcaatgtccc ctttcttagt
     4741 tcgaattcga atatggagtt tcttatcctt ataaagatag gcatactccg actcaggcct
     4801 atggtaaata gcactcaatt ccatcaattt gtctcctaaa gtattcattc aaattttacg
     4861 caaacgtttt caaactcaat aaatccagta tactattttt gtaaactatt tgcaaggaat
     4921 gtccgttgtt aaaggaacta tcttttaaag tatacaaaaa gccttaaaaa aggctttctc
     4981 tatatactaa tcctctttcg gttttgaaaa tacttctact cgctgagcta ggactttgat
     5041 ttctacaggt aggttatctg atttatctcc gtcaacatct gactccaatt cactatctga
     5101 agagatttta atattacgcg ctttgatata ctcgatatta tcatttgcga caacatcccc
     5161 ttttaataaa tcaggaatga cggataattt ggagaatata gaggcatctt tcagaatcaa
     5221 aatgttggca tagccgtcct tgttttcttc aaagattttc ttatcagcga agtaatttgt
     5281 caaaaggacc aaaacatggc tagcttcacc aacataattt ccattttctg tctcaacctt
     5341 aatgttaaag acctgatctg tcatgacaga cttcatggta tttacagcat aggttaaaat
     5401 accgaatttt gttttgtcct ctatttcaac attgtgaatc gcctcaggca gagaaccgat
     5461 actaaagata taaccaaaat agttgtcatt tgctttaccg atatcaatct tattggttaa
     5521 atcaaaatcc agttcgtcaa ttgcgccatc gatgtcttga ttgatttcca aaagttttgt
     5581 aatgaggtta cccgtaccgc ctgggataat ccctaactta ggaatgtagt ctctctcatc
     5641 aatacctgaa atgacttcat tgacagttcc atctccacca aacacaacca ctgcatcata
     5701 ctgctcacga gaagcttctt cagcaaaatg tgttgcatcc agcgcttttt cggtaatttt
     5761 ggtttcaaca tattcaaagt attcttttgc tttattctcc agcttttctt tgtaatccaa
     5821 agccttctcg ccaccagaag tagggttgat aattaccatt gcttttttca ttgattttat
     5881 ccttaatttt aaacagaaat gtttacattt cgtcgtatgc aagtaaatgt aatcctatta
     5941 tacaatgaaa atacagaaaa gagaaatctg acgtactgga gattaatacg cttttattct
     6001 attttcccat cgcctaacta catcctttaa gggttcatcc aagtaagaat aggccttatc
     6061 cttgatccaa tcaggaatac cgtaagctgc ctctgctatg ttacaagtga ttgctgcgag
     6121 agtatcactg tcgccaccaa gtgagatggc atttcttatc gcatcttcga agtctctact
     6181 ttcaagaaag gcgataatgg cttgagggac agtttcctga catgtttcgt taaaacgata
     6241 gttaggacgg atttcatcta aagtttgaga tagattgtaa tcgtattctt tttctatata
     6301 atccttaatt cgtcttttac actctgtaag attgtcgtta attgattgct cgtactctct
     6361 acaataacca ccaaagtaaa aacgacatag aaagatagca tcagctgtag ccatagcgcc
     6421 tttgacacct tctggatgat tatgagttac ctctgcagaa agactcgtaa gtcctctaga
     6481 tgatggccat ataccagttt tcgcataaaa accacagtcc atgatccaag cacatggaga
     6541 aatacgcata gctgatccat tcccaaagct attataaggc tcacggttat cgctgtttag
     6601 ccatgcatta aaccgagcac cgtaatcagc attcggatac attctgccat atttcttcat
     6661 cgcgtcaatg aaatcgtctt tttgtccacc attcataatt gcttctgcaa cagcacaggt
     6721 cataaccgtg tcatctgtaa aaaagcagtc cttccgaaat aaaggaaagt cctttgtttt
     6781 gatattgttc cattcgtaaa cagaaccgac aatatctcca ataattgctc caagcatcag
     6841 attcctcctt gttcattttg atgcttttta tattggttat ataccatatt tattttagaa
     6901 aacaacatcc tgttggattt taaaaatttc atttttttca aaatagggtt ttaccatttc
     6961 tttccaccta gctctatgaa aattgattga ttttaaagga gatagggcat aatttcccaa
     7021 tgcataacca tcatttactt caacaacaag tgttctgcca tcgcgagtaa caccgatatc
     7081 tagtccataa gctattggcg catctttcca acatgatatc gcttcatcaa ttacacttgc
     7141 atcaaattgt gcatgataat cacctgtata gggtcgaaca tctaatacgc gaccatctaa
     7201 cacaaaacaa cgccattcag ctatgaattc tacaacctca ctaatccata taggatagtc
     7261 gaaaggtaga ccaataccta ttaaatcatg ggttccatta acaactcttc cagtaaagac
     7321 ttttgaacca gctttaggct taataaattt tccccaatta tcaggtatat tcacaatctc
     7381 tcctaaaata ccagcataaa tctttcgacc ataaaactct ttaagctcaa taggatagtc
     7441 ataaaccgga acgtttaagc ccatcatttt tagtaatgct ctagtctcca ttatataatc
     7501 tacaactata tcttcacttg ttaactcttt tatttcagaa aaagattgat ataaaataac
     7561 ttcttctcct tgtaagtagg cacctacttg agcattgtat ttattaattg aaacctcact
     7621 tggtaattta ctttgtctaa tataaacaac catttcatca ctcctatatc actagtgtta
     7681 cactgatttg taaaaaataa tagcaatttt gctcttattt ttttgagtaa atagctccca
     7741 taatatcatc gaaataatca acggtattta ggagtaattc aataacctgg gactttgtta
     7801 gtcgcattcc ccttctatct ctagcatctt ctactaaatt ttcaagtttc tctagatttt
     7861 tatcatccaa gctaatcatt attctatttt tatcggttgc cattttcatc acctcaagtt
     7921 aattctatca caggtgtaac acttgtgtca actggctttt ataatacatt agtttaaaag
     7981 tggagaggat ttttaacaca gtaactttaa atctttggta ttaaaaaatt ttcacaatat
     8041 ttatagaaat aaaatctgtc tcaaatcagt tatcaaatct agtataaatt atgagcggct
     8101 actctaatac tttccctcta aacaagaaaa agacttacac tcaaggattt tcttcccccc
     8161 cttcgttata acgttttgac tcttttacta gcaaaggtat atactcacaa ggaactttgg
     8221 ttgactattg aatctctcca acttcttctt taacatatcc ttctacatct tcaatctcta
     8281 caaacattgg gtctaagtga cacaagaaat gccaaacttc gatccctttt tttctgtaaa
     8341 gaatcgcttc accgtcttca cttccgaaaa agcttctgtc gatttcatat ccgcggcttt
     8401 ctaagaagtc ttttgcttta cgatagttcg tttctcttgt ttcgacatag gttttaactt
     8461 catggttgtt aacgacatat gcatcaattt ttgaatatcc ttcgatcact ctatcatttt
     8521 tgagggataa atttgaaatc tctttccaaa taatgtttac attttcctca ggatcgaaca
     8581 taaatttaga taaaggaaca atatttccgt taaaaataat ttccatataa tccggtatgt
     8641 ttttaggatt aaaatactcc acttcaaaac catcttctgt ttccagagtg tatcccggga
     8701 tttgagctac aaaggctttc ccatcttcta tggaatcaaa tgctactaaa tctttagaat
     8761 aatcattttg gtacaattcc aatataacca tcgataatct ctccattttc attatcaggc
     8821 taatgtaaat aagcacgtca cctgaccaat tcaggctctc tgtatcatct catcatattt
     8881 cctacttact ttacgagtcc tatacccaga acacacctta tcgaccttcg gtctcacctc
     8941 gttgcattgg ctgaacatct acttttactt cgctgatgct tcaactcgta caagcagtga
     9001 taccgcctca gcgtgatgcg tcagtgggac tcaaaaggtt cggggaacct tttgaggatt
     9061 aactacgttt ctctaataaa cttacacatt caacttgttc atcattgtcc aaacctatgt
     9121 tgagattttc ttctataatt ggtagcttaa aagtaatgga ttttagccat tgtccgttag
     9181 attgtttttc ttcataaact tgaatttcag aaatcaaagc tgaaattaac tgcctacgct
     9241 ctacatcatt catgacttta tagagcttat caaaatagat cagaacctta tatatgttat
     9301 ctcctgtaag cttttcagct tcaatagtct gtttctttgc tttcgcatca attagtgatg
     9361 attctaattc atctactttg tcatacatac gatatagtct atcatctaaa tcctgtttcc
     9421 ttctcttata atgcttatct tcaacatcta aattatctat ttcctcaatt agcttaaact
     9481 ttgtagaatg actctttctc aattcctttt ggtaattatc tatttctttt tctatttcag
     9541 aggtatccac cttcatgttg attttttctt gcatcataga agcaaatttc ggattactta
     9601 ctatcttgac aatcacctct gcaacagcat catctaacaa ttcttctcta atttgcttac
     9661 tgaatgtaca cttatgacct cttatcatct gcctatggtt acaaccatag taataaaaat
     9721 ctttatactt tgtgccatct ttctttttct tgatacactt gttcccaaac attcccactc
     9781 cacatatcgg gcattttaca attccagaaa gcaagtgtgt gcgtgtatct tttcctttat
     9841 tcacatgctc atatttcttt gcttgagatt ttagcttaac ctgagcagct tgccaaactt
     9901 catcggaaac tatagcttca tgtatccctt cagatattag atattcatct tgttcaacct
     9961 gcttatattc atttcttgta ccatgaactt tttctaaagt tcttcttcca aatgctattt
    10021 tcccattata tacaggattc tttaatatct ttcttataag acctgcatca aacaaaggat
    10081 tcttaccatt ctgtcttggg atttttctaa ttccatgatt ctctaagtat ttagatatcc
    10141 cattggctcc tatcgtagta tttacatact ggtcgaaaat cgttcttatt gcaactgcct
    10201 cttcctcatt tataaacagc ttgccgtctt caagtttata tccatacgga gcaaagccac
    10261 cattccattt tccttccctt gctttttgaa tgcgaccttc cattgtttga atacggatgt
    10321 tttctctttc tatttcagcc acagctgata aaacagaaat cattagtttc ccagcatctt
    10381 tagatgaatc aatgccatct tcaacgcaga taagattaac tccataatcc tgcattatct
    10441 gaagtgtaga aagaacatca gcggcatttc ttgcaaatct tgataactta aacacaagaa
    10501 caaaagatac tccatctttt ccagatttta tatcttccat cattcgattg aactgtattc
    10561 taccttcaat agacttgcca gacttcccgg catcttcata ctctccaaca atttcataat
    10621 cgttgtaaat agcaaaagct ttcattcgtg atttttgtgc ctctaacgaa tacccctcta
    10681 tctgtattga cgtagatact cgtgtataga ggtatacttt tattttttct tttgacatag
    10741 tattaatctc aatataattt ttctatatca tatataattt ttttaattta agtttggact
    10801 atcatttcaa gtatattata acacttttat tagtccgtct caatttgtgt ttttgccatg
    10861 tcaaaactat ttttcatctc ttgatttttt gctggcgttg gatcgggtag attatctaaa
    10921 tctaaagcac cagcatattt tgcaatcaga tttgctatta aatcagccaa tccattccag
    10981 tcattgtcca atatatacct cctctaaagt tttatatcta ataattattt gtttaattaa
    11041 gttttttgac attgacaagt gctttggatt agcaacatag gaatctcacc tccgcctcta
    11101 ttccggatga gccggcttca accttagaag tatcattatc ctcattttct tcatagcgga
    11161 tagggtatcc ctccctatat tcaaactctt acttatcgct cactttcttt ttacttagca
    11221 gaactttttt tgccgaatta ttcagccgaa agatcttgac ggataggtta ttacgctcca
    11281 aaaataatta acgtcttgtc ttggtctatt caattgttaa ggttcaaaat ttatcgagag
    11341 ttattaatct ttttaaaatt tgaccatcag aaaatattta tcttaatgta acaaaattct
    11401 ataaattacc ctcttatact taacagtgaa aagaagtctt tcttggtaac caattttgaa
    11461 atagaatttg cttatataaa aaggtcaaat tcccactgca taagtagcag tgaaaattag
    11521 accctcttgg taactgtcat ctaaaagtct tctagcattt ctttaatttt ctaatcgcct
    11581 ttaaagtgtc atatttatat ttttttatct ctggcatact ttgatacctt tttagactta
    11641 aagtctttaa tagtgccttt ccacctcttt ttatctataa agattctcct acatcataat
    11701 tcattttttt atttaaacct ttctgtctta gtttgtcttt atcttcttca taccatttta
    11761 agattgtcac atagtggttt tgataggtct taccactgct ttccatgtat ctggatagtt
    11821 tatttatcat tatatctgtg tgtgagttta atttttcttt tagattttta tattcttctt
    11881 tgcttaacct tacatttttg aattctccat aaaaaatggg ggtggacttt ttatctatct
    11941 ctccctctct ctctttatct atctctatat ctttccatgt aattccaatc tggagtacct
    12001 ctactgtcta tcggtaattt aattttgata tctggcaata ctgtgctaga tatttgatct
    12061 ttatattcag tattttttaa agcttgccta ataattgaag ttaaatagaa tgctacttct
    12121 ttattcaatt ctttattttt taattttaaa caatgaattt tcatatctag gcttgcttta
    12181 tatttatgat aaaagactgc tcctaaaaat gaaacagata taaaattttc aaaaactcta
    12241 taatttttat catctatatc ttcgtagtaa cctaagatac cattgtcaat atttgtagca
    12301 ctaattctag gagtttttcc atcgagtaaa tatctttttg gaatagatga gcctgttggt
    12361 acttgactcg atttcccctt tttttcggta ataaatattt ctttttattt tgttgtctga
    12421 tatttttcct acctgtcctt tgtaggatga gtattttcta gattttcttg aataactttt
    12481 tacttgaagt tttagctttt gaactagtcg ttgtactttc tttttgttta ttatcagtcc
    12541 tgatcttttt aatattgctg ttattctcta tatcctattt ttcattcatg atattctttt
    12601 actaatttta tcttaaattc tgtgctgtat ttgccattaa aaaactgacc tcctttagtt
    12661 agttttttgg cctaactttt gagggtcagt tcaaaatttg cgacttttaa atgaattcca
    12721 atattcaatt attaagagct aacatggtgc ttgccaatag gaatcattag aggcgaattg
    12781 gaaatagggt cacgtataat ttttgcttca agattaaaga tatctttaac tagtttatca
    12841 tttagtatat cttcaggctt tccctctgca acaagtttac cttctttaat tgcaaatagg
    12901 taatcagcgt atcttgctgt tagatttata tcgtgcaaaa tcatgcaaat ggttgtctta
    12961 tatttttggt ttagatcagt caagaggtct aatagttcta tttgatatga gatatccaag
    13021 taagtagttg gctcatctaa aagtaggata cttgtatctt gggctagggc tagagctatc
    13081 catactcttt gcctttgacc cccagaaagt tcttcaacta ggttatttgc tagatcttca
    13141 acattggcct taaccattga tctgtttatt atttcaaggt catcttttcc aagactctta
    13201 aaaggctttc tgtaggggaa acgaccacgg cttacaagat cagctactgt tattgattca
    13261 gggattattg gagattgagg taatatagct atgtgttttg ctaaatcttt ttctttataa
    13321 gaattaattg atttattatc aagcaatact tctccctcta atggctttat aagtcgagac
    13381 aaggttttaa tgagtgttga tttcccacaa ccatttgacc caataataac tgatattttt
    13441 tcttcaggta tttttatatt tatattttcc aagattattt tttcatcata accgcaggta
    13501 agattatttg accacagacc tttcattata tattcctcct gttcattttt attagtaagt
    13561 atattaagta tggtgaacct aacaagccag ttacaacacc tactggatat ctagctggta
    13621 aaatattttg agagaatatg tctgataaca aaactagtaa aattccaacc aatccagcta
    13681 atattgggct tcttttcttg ccaatattta aggctatggg accagctaaa aaagatatac
    13741 aagctattgg tcctgtaatt gaagtagaaa aagcagttaa agatacagcg caaaaaatta
    13801 aaacaagcct tgaaagctcg ggatttgctc caagtccgat tgctatttct tcaccaagtt
    13861 caataatttc tagtctttta ttaaaaaata aaactaatat agtagcaata atacttacta
    13921 ttagaacaag aggtatgtca tctaactttg taaaagataa agagccactg agccatctca
    13981 taacttcttg taattcatat cttgctactt tcaacaataa aaatgaggtg cctgctcttg
    14041 tgacagcttg aaaaccaata cctaatatta tcagtcttgc tgctgaaaaa ccatcttttt
    14101 tagctagtaa aaataatatt aaagatgatg ttagtccaca agttattgaa ataattccag
    14161 tagttaaact atttgttttt aataccaata tgcaaaagac cgctgcaata gatgaagaac
    14221 ttgtgacacc gattatatca ggacttgcaa gaggatttct taacatagtt tgaaagataa
    14281 atcctgccaa tccaaaagac cagccagcta taattcctgc taataatttt ggtaatctaa
    14341 tttccataat cgaaaaacta gctccaggaa cagtttcact atttaagact ttaatcaaag
    14401 ttgaaaaaga ataactttca tctccgataa gtaaaatgaa aaatgataga ctgattatta
    14461 ttaataaaaa tagtgaggaa aatagtgtta ttctattttt tcttttttga atacctataa
    14521 ttaaattttg cattagttat taacccctct atttttcata gttacataaa taagtactgg
    14581 acccccgatt attgcagtaa ttatccctac ttcaatttca cctggtttac ctaacatacg
    14641 gccgattata tcacatataa gcaagagctc tgcacctata aaagatgaag aaatggtcat
    14701 tgtgcgtata tctttgctta taaataagcc acaaaagtga ggaactataa gacctacgaa
    14761 gccaataggt ccaccaattg cagtaatact tgaacataaa agcacacttg caattattgc
    14821 aagtgatctt atcctattaa cattaactcc aagaccaaca gccatttcat cacccatagc
    14881 taaagcgttt aaatctgatg aaataaatat agctatcaag tgacctaaaa ttataaaagg
    14941 tagtagtgta gatatagaag ataatgtagc tgctccaagg ctacctattt gccaaaatct
    15001 aaatttgtct aagacgttat tattcggtaa aattaaaaaa cttacaaaac tgcttaaagc
    15061 catactaaca caagttcctg ataaggctag ttttataggg gtaaggcctg cttttccgct
    15121 tacagcaatc gcgtatacaa aaattgcact tactaagcca ccaatgattg caaaacttat
    15181 atggcttatg cttgatgaaa ttcctaaaaa agaaagacca attactacac taagacttgc
    15241 tcctgtgttt ataccgagta tacctggatc agctattggg tttctagtaa ctgattgcat
    15301 caatacaccg cttatggcaa gactagaacc tgctaaaatt gcaaaaatag ttctaggtat
    15361 tcttttatat ataattgatt taataaaatc atcatttgta ttgcccattc caaaagttgc
    15421 taaaaaatct ctgatattaa tttcttttgt tccaagcttt aatgacaaaa atattgtaat
    15481 aagaagacct aggcagatta caaaaattat gcctaggttt atttgttttt tatttcgcat
    15541 ttttgcatgc atttcctaaa agatttaggt attcttcaat agtatagttt attgaaagtg
    15601 gtgttggagt gcatgaggct gctaacggtg tattatctgg aattacagca acggcaccat
    15661 ttttaattgc atttatttta cctaaaagag gatctttttg taaagcttca agagttttat
    15721 catcaccata agttatgatt acatcagcat catttatctt atttgcttct tctgcagaaa
    15781 tttcctttgc aaaactatct tcactctcaa attcttttaa tgattcaggg aaaactagac
    15841 ctaaatctgt taaataattt gctcttggat ctttgctagt ataaatccag aattttgatg
    15901 tatctgcagc attaatcata gtaaataata ctttttttcc tttgattttt cctttgattt
    15961 ctggatgttt ttctaattct ttggatatac gagcttcagt atttttgatt aactcatcac
    16021 cttctttttc catacctaag gcttttgaat caattttaat catatctctc cataaagttt
    16081 gccaaggttt agatttgtat gctgctacag gagcaatttt tgatagagtg tcataatctt
    16141 ctttagttat accagaataa cctgctaaga taacatctgg tttagaattt gatattgctt
    16201 caaagttaag tccatccaaa tcgtcaaata ggttagcttt accatttagt tctttgattt
    16261 tttcttctgt ccatggtaaa actcctttat cagcacttac accgtaattt gcttttgaaa
    16321 atccaacagg aactattcct aaagctaatg ctacatcatg atttccccaa gcaatagttg
    16381 caactctttc aggtttttta tctaatatag tttgaccgaa agcatgatct aaaactattt
    16441 tatcaggcgc atgctcttta gaagtatttt cttcattttt aacagaatta gaagaacaag
    16501 cgctaaaaac tatacacata gctaaaatag ctattagaaa aaatttgttt ttcataaaaa
    16561 ctccttaaac atatttcaag tctattgtat cgataataat tatcattgtc aatagataca
    16621 gttacaaatt taataatata attttttcaa aaaagg
//
LOCUS       DAVBCR010000042        16463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCR010000042 DAVBCR010000000
VERSION     DAVBCR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.042"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(46..2043)
                     /gene="metG"
                     /locus_tag="VQ508_001301"
     CDS             complement(46..2043)
                     /gene="metG"
                     /locus_tag="VQ508_001301"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2490295.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2167..3426)
                     /locus_tag="VQ508_001302"
     CDS             complement(2167..3426)
                     /locus_tag="VQ508_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490296.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3428..4129)
                     /locus_tag="VQ508_001303"
     CDS             complement(3428..4129)
                     /locus_tag="VQ508_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490297.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4113..5312)
                     /locus_tag="VQ508_001304"
     CDS             complement(4113..5312)
                     /locus_tag="VQ508_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2490298.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5598..6944
                     /gene="gor"
                     /locus_tag="VQ508_001305"
     CDS             5598..6944
                     /gene="gor"
                     /locus_tag="VQ508_001305"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2490299.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7141..7677
                     /locus_tag="VQ508_001306"
     CDS             7141..7677
                     /locus_tag="VQ508_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2490300.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7728..7814
                     /locus_tag="VQ508_001307"
                     /pseudo
     CDS             7728..7814
                     /locus_tag="VQ508_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7883..8098)
                     /locus_tag="VQ508_001308"
     CDS             complement(7883..8098)
                     /locus_tag="VQ508_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2490301.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8196..8516
                     /locus_tag="VQ508_001309"
     CDS             8196..8516
                     /locus_tag="VQ508_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490302.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8547..8885)
                     /locus_tag="VQ508_001310"
     CDS             complement(8547..8885)
                     /locus_tag="VQ508_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490303.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8897..9616)
                     /gene="trmD"
                     /locus_tag="VQ508_001311"
     CDS             complement(8897..9616)
                     /gene="trmD"
                     /locus_tag="VQ508_001311"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2490304.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9606..10124)
                     /gene="rimM"
                     /locus_tag="VQ508_001312"
     CDS             complement(9606..10124)
                     /gene="rimM"
                     /locus_tag="VQ508_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2490305.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10155..10943)
                     /locus_tag="VQ508_001313"
     CDS             complement(10155..10943)
                     /locus_tag="VQ508_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2490306.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(11520..11759)
                     /locus_tag="VQ508_001314"
     CDS             complement(11520..11759)
                     /locus_tag="VQ508_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2490307.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(11779..12051)
                     /gene="rpsP"
                     /locus_tag="VQ508_001315"
     CDS             complement(11779..12051)
                     /gene="rpsP"
                     /locus_tag="VQ508_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2490308.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            12221..12430
                     /locus_tag="VQ508_001316"
     CDS             12221..12430
                     /locus_tag="VQ508_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490309.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            12909..13130
                     /locus_tag="VQ508_001317"
     CDS             12909..13130
                     /locus_tag="VQ508_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490310.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            13213..14016
                     /locus_tag="VQ508_001318"
     CDS             13213..14016
                     /locus_tag="VQ508_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2490311.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(14072..15613)
                     /locus_tag="VQ508_001319"
     CDS             complement(14072..15613)
                     /locus_tag="VQ508_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2490312.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(15615..16097)
                     /locus_tag="VQ508_001320"
     CDS             complement(15615..16097)
                     /locus_tag="VQ508_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2490313.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(16097..>16463)
                     /locus_tag="VQ508_001321"
     CDS             complement(16097..>16463)
                     /locus_tag="VQ508_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2490314.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         4825 a         3577 c         3021 g         5040 t
ORIGIN      
        1 tataaataaa aaaacagaac tagtctgaac tagtcctgtc tacttttacc caatcacact
       61 tccatttggt acagctggat caactgtgag aagggttaat ttgccatcat attcagctga
      121 tagaatcata ccctggctga catatttttt catcatttta cgtggtttga ggttagcaac
      181 gatttgaact ttcttgccga ccaattcttg ttcatttgga tagtattttg caattcctga
      241 aagaatctga cgatcttctc catcaccagc atccaagcgg aattgaagca acttatctga
      301 accttctact ttagacactt ctttgacttc tgcgacacgg atttcaacct tgtcaaagtc
      361 ttcaaacttg atttcatcct tgtttagttt gagctcaact tcgtccggat tccattcttt
      421 ttcgactgct ggtttattgc cttccatttg ttccttgata taggcgattt cttcttccat
      481 atttagacgt ggaaagatag gtgttccttt ggcaactaca gtcacatctg ctgggaagtc
      541 agccaaactc aagttttcaa gactagaaac ttcttccaaa ccaagttgag tcaaaactgc
      601 acgactagtt tccatcataa atggttcaat caagtgagca actacacgaa tgctggctgc
      661 caagtggctc atgacacttg ccaattggtc acgaagagct tcatccttgg ccaagaccca
      721 tggtgcggtc tcatcgatgt atttattggt acgagagatc agagtccaga ctgcttcaag
      781 cgcacgtgga tagtcaactg cttccatgtg tgtatggaag tctgcgattg attgttctgc
      841 aacctcagca agaacatgat caaattcagt cacaccttct acataggcag ggatttgtcc
      901 atcaaagtac ttattaatca tggaaaccgt acggttaagg aggttcccaa ggtcattagc
      961 caattcatag ttgatacggc cgacatagtc ttcaggagta aaggttccgt ctgaaccaac
     1021 tggaaggtta cgcatgaggt agtaacgaag tggatctagt ccataacgct ctaccaacat
     1081 ttcagggtaa acgacattcc cttttgactt agacattttt ccgtctttca tgacaaacca
     1141 accatgggca atcaaacgat caggtaattt aacatccaac atcataagaa ggattggcca
     1201 gtagatagag tggaagcgaa ggatatcttt tcctaccata tggaagactg ttccattcca
     1261 gaacttgtca aagttaccat gttcgtcttg agcgtagcca agagctgtcg catagttaag
     1321 aagggcatca atccaaacgt agacaacgtg ttttggattt gatgggacag gcactcccca
     1381 tgtaaaggtt gtacgagata ccgccaaatc ttccaagcct ggctcgatga agttgcgtag
     1441 catttcatta aggcgaccat ctggcgtgat aaattcagga tgagctttga aaaattcgac
     1501 caaacggtct tggtatttgc taaggcgaag gaagtatgat tcttcagaaa cccattcaac
     1561 ctcatgacct gatggagcaa taccaccagt cacatttcca gcttcatcac ggaaaacttc
     1621 tgccagctgg ctttctgtaa agaattcttc gtctgatact gaataccaac cagagtattc
     1681 acccaagtag atatcatctt gagcaagtaa gcgttcaaag acctgtgcga caactttttc
     1741 atggtagtca tcggttgtac ggataaattt atcgtatgag atatctagta attgccagag
     1801 ttctttaact ccaaccgcca ttccatcaac ataggcttga ggtgtaatac cagcttcttc
     1861 cgctttctgc tggattttct gaccatgttc atcaagacct gtcagataaa atacatcgta
     1921 gcccatcagg cgtttgtaac gtgctaggac atcacatgcg atagttgtgt aggcagaacc
     1981 gatatgaagt ttcccagatg gatagtaaat cggcgttgta atataaaaat ttttttcaga
     2041 cataattttt cctttccagg caaatgaaac ctgtttttct aacacttcat tatatcacat
     2101 ttttaatgaa tttcgatagg gaaatccata caaaaacaag atagacgagt gtccatcttg
     2161 ttgatctcat tcataacgaa gggcttcaat tggatcaagt ttcgatgcct tgttggctgg
     2221 caagactcca aaaatcatac caacactagc cgaaactgca agactaaata gggcaactgg
     2281 gattgatact ccaacttcta taccttctat taaaccttgc agtaacaaac ctgctaaggc
     2341 agttaaacca cttgcaattg tcaagccaat taagccacct aacaaggtca aaatcatgga
     2401 ttcaatcaaa aactgaatta aaatattggc acgtgttgca cccaaagctt tacgaagacc
     2461 aatctcacga gtgcgctctg tcaccgaaac cagcatgatg ttcatgacac cagttcctcc
     2521 aacaaagaga gaaatccctg cgatggaact aataatcgtc gtcataaaac taaacgattg
     2581 ttgaatttct gcaaatacaa cggactcatc tgccacctgg tattctccct gttgtaagcc
     2641 tgcaagctct gtcatttttc gtgccagttc tggacccaga gttggggtta aactggtatc
     2701 attcactcga aagacaatac tagctatttc atctatatta aaattcgcag caagggagat
     2761 attggtagta ataggcaagc caccaaaccc atatattttt gactttttag cctccggact
     2821 agtataaacc ccaatgaccc ggtaactaaa tccattgact tctacaacct tgttaatagc
     2881 ctcttgagga gattcaaata aactaatgga caattcctca tctagcaaaa taacacttgc
     2941 aaactctttg aaatcttgct ctctcagact acgacctgca ataatttcat tcttaacagc
     3001 gtccatgtaa gttctgtttc cacctgtcaa attagcattc tcaacctttt tatcttgata
     3061 ggtcaagatg gcattcgttg aattggttac atagtaacta tccactccct tcagtttagc
     3121 tgcctcttga acccaggatt cttgcggttt tggcggttca acaggaactt cctcttcctt
     3181 tccagaaacc gtaaaagctg attgtttctg agtaaaagac ccatctttac tttttttagg
     3241 agagaaaaag acgctaatat ttttctgaga tttagtcata tctttattga cttgacgaga
     3301 tagggaatca cccaaagcca taatcacaac aactgatgaa acaccgataa taatcccaat
     3361 catagtaagc aaagaacgca tcttgtgagc catgatagat gaaaaggcaa atttcagatt
     3421 ctgcatctta gttttcctcc tttcctaact gagcactgtc agacgaaatg accccatccc
     3481 gaatgacaat ctgacgtttg gcataggcag caatctcagg ctcatgcgtt accatgataa
     3541 tggtttttcc ttctttattc aaatcaacca ataattgcat aatttggtta cctgttttgg
     3601 tatccaaggc tcctgtcggt tcatccgcta ggataataga aggattgttt accaaggcac
     3661 gcgcaatggc tacacgttgc ttttgaccac cagataattc tgaaggtaaa tggtgactac
     3721 gttctgtcaa ttcaaccttg tctaaatatt cctcagccaa cttgcgacgt tttgaagacg
     3781 aaactcctgc gtaaatcaag ggcaattcta cattttgcag agcattgagc ttcgatagaa
     3841 gaaagaactg ctgaaagaca aaaccgattt gttggttacg gaccttagct agttgttttt
     3901 caccaagccc agccacttct tgaccttcaa gataatattc tccactggtt ggtgtatcca
     3961 acatgccaat cgtattcatc agagtggact taccagaccc agatggtccc atgatggcta
     4021 caaattcacc ctcattcact tctagattga tatttttgag aacctgcagt tcttggtcac
     4081 cattacggta acttctgaag atatttttta gactaattag ttgcttcatc agccttcacc
     4141 tcttttcctt cttccaagga agatgttgga ttactgatga ccttagcacc gttcgttaaa
     4201 ccagaagtga tttcttgatt ttctgcgtca gcatttccca atgaaacctc aactttttta
     4261 gccttttgtt gttcatccac aatccagaca taatttttac tatcatccat tactagactg
     4321 ctaacaggaa taagaatagc cttagttttg cttttaacct caatgttgac agaaaaacct
     4381 tgtttcaaat caccaacctc gcctgtcaca tcaatagtat aagggtattt agaacctgta
     4441 ttattcccgg ctgctggact agctgcttca ccattgtttt taggatagtc agaaatatag
     4501 cttaatttcc cagtccattt tttatcagga tacactttag aagtaaagct tacttcttga
     4561 cctacagaaa ggttggctag attgtactca gacaattctc ccttgacttg taaattttca
     4621 ttgctgacaa tatgaaccat aacttgactc gcccctgttg gagatttaga aacattgcta
     4681 ttgacttcga ctacagttcc ctctagggta ctgagaacag ttgttgcatc caattgactt
     4741 tgagccttgc ttaattgcgc tgcagcatct gcacgcgcat cacgggcatc acccaattga
     4801 gcatcaatag aagcaacaga atttccagcc actggagttg ggctttgcac cgttgcatct
     4861 tctcctccta ctggcgctgg taactgtgga gcctgagctg aagcggcttc atttcgtgct
     4921 tgattgagtt cattgatatg acgatctgcc ttagctactg ctcgactagc tgaatcatag
     4981 gccgcctgcg cttctgaact actgtacttg actaaagcct gcccttcgct gaccttatcg
     5041 cccacagaaa caaggatttc atctaaatca cccttactag catcaaaata aacatattgt
     5101 tcattttttg ctgttactgt ccctgacaat aaaacagagg atgccacgct tccttccttg
     5161 gcaacaacaa gatgagtagg ctcatctttt acagcagtct gagaaggttg tctaaaaagt
     5221 aaaatccccc cggcacccaa tacaactaca ctcgcagcac cgattgctgc atacagttgc
     5281 cactttttag ctttaccatt ctttttcttc ataatgaaac tccttttctt ttttacaata
     5341 ctttgctatt ataccaaatt tccctccagc aaacaatgca gttcagatgg aaataatggc
     5401 agttcaggat taaacaatcg ttcggaattt tgcttttcta ttgtactaga tatataatac
     5461 actatcttga cttatttggc aagctttttt gcaatttttt gtgcgtagca gatttttgca
     5521 atcaaatcaa aaaaagaata aaatataact atcaaaaaag acactctact atttaaaaga
     5581 ttacaaagga gttttcaatg agagaatatg atatcattgc tatcggtgga ggtagcggag
     5641 gaatcgctac catgaaccgt gctggtgaac atggagccaa agcagccgtt attgaggaaa
     5701 agaaattagg tggaacctgt gtcaacgtcg gttgtgttcc taaaaaaatc atgtggtacg
     5761 gggcgcaaat cgctgagact ttccatcaat ttggagaaga ctacggcttt aagactactg
     5821 atcttaactt tgactttgca accctacgtc gcaatcgtga agcctacatt gatcgcgctc
     5881 gttcttctta tgatggtagt tttaaacgca acggtgtaga cttgattgaa ggtcatgctg
     5941 aatttgtaga ttctcatact gtaagcgtaa atggtgaact gattcgtgct aaacatatcg
     6001 tgattgctac aggtgcccat ccaagtattc ctaatattcc tggtgctgag ctaggtggct
     6061 cttctgatga tgtatttgcc tgggaagaac ttccagagtc aattgccatt ctaggcgctg
     6121 gttatatcgc cgttgaatta gctggcgtac tccacacttt tggtgtcaag acagatctct
     6181 ttgttcgccg cgatcgtcct ttacgtggtt ttgattccta catcgttgaa ggtttggtca
     6241 aggaaatgga aagaacaaac ttaccacttc acactcacaa agtccctgtc aagttagaaa
     6301 aaactactga cggcattacc attcatttcg aagatggtac tagtcacaca gctagccaag
     6361 ttatctgggc tacaggtcgc cgtccaaacg ttaagggctt gcaacttgaa aaagctggag
     6421 tgactctgaa cgaacgtggc tttatccaag tggatgaata ccaaaatact gttgttgagg
     6481 gaatctatgc tctaggtgat gtaacgggcg agaaagaact gactccagtt gcaatcaagg
     6541 ccggacgtac cctatctgaa cgtctcttta acggaaaaac tactgcaaaa atggattact
     6601 caactattcc aactgttgtc ttttcacacc ctgctatcgg aactgttggt ttgacagaag
     6661 agcaagctat taaagaatac ggtcaagacc aaatcaaggt ttataaatca agctttgcat
     6721 ctatgtactc tgcttgcact cgcaaccgtc aagaatcccg tttcaaactc ataacagctg
     6781 gttcagaaga aaaagttgtc ggacttcatg gaattggcta cggcgttgat gaaatgattc
     6841 agggatttgc cgttgctatc aaaatgggag caaccaaggc tgactttgat gcaactgtgg
     6901 caattcaccc aactgcatct gaagaatttg taaccatgcg ttaatcgaaa caaaagaagc
     6961 tgatataaat atgtctgctt cttttttaat gattttcgaa tcaacaaaaa aattatccaa
     7021 cttgactagt cacgaaaatc ggctgttaca gtttttgtta ccttgttttt aaaaaatggt
     7081 tgacatcgac tcctatatga gtataatagt tatcatacat tagaaaagag gttaactatt
     7141 ttgaaaaaag ctcacgttta tgctatccct gctattgggg ctgctctcat tgctgctttg
     7201 gcacaaatca gtcttccaat tggacctgtt cccttcactc tgcaaaactt tgcaatcggc
     7261 ttgattgcta ctgtctttag accgagagag gctgtacttt ctgctggact ctatcttctt
     7321 ctaggtgcta tcggtcttcc tgtctttgca ggaggtggag ctggttttca ggctttagtt
     7381 ggccctactg caggctatct ttggttttat ctcgtttact ctggacttac ttcctctcta
     7441 accaacagca agagtggtgt tgttaagatt tttcttgcaa acctcttggg tgatgccctt
     7501 gtctttgtcg gcgggattct cagcttgcat ttcctagctg gaatggcatt tgaaaaagct
     7561 cttgctgtgg gggttcttcc ctttatcatt ccagaccttg gcaaacttct ggctattagt
     7621 tttattagcc gtcccctact tcaacgcctt aaaaatcagg cttactttac taactaaaaa
     7681 aggatatcga gttatcatga ctcaatatcc ttttctttta ttttgaaaac ttatactcaa
     7741 tgaaaatcaa agagcaaact agaaagctag ccgcaggctg ctcaaaacac tgttttgagg
     7801 ttgtggatga aactgacgaa gtcagtaacc atacatacgg caaggcaaag ctgacgcggt
     7861 ttgaagagat tttcgaagag tattagttgc ctttaaaggc atccaccatc gtttgaaatt
     7921 cttcatttga gagagtaatc cctttgccca ttttagtatg gtctggactc caagcacgaa
     7981 tatcaaactt tgcaggggca ccattaaagc tcacacggtt aatttccttg gtccaacctt
     8041 tttcgttttc agaaagagtc aacaagtgct cttcgatttc aaatgtaaat tctgccattt
     8101 tcttctcctt ttttagtttc attagtttat tcgtaaaatc ttgtagattt taggaaaatt
     8161 ttatataata ttgatataaa agaagggagg ccaatatgag acataaattc cagcaagttc
     8221 taaataaaat acatgatttt ttaaatggat atgaccaacc tgaccagact gaaaccaact
     8281 cccttacagc cactattgaa gaggctatcc agaaacaaac cgctgttcac cttatcttgt
     8341 ctgagacaag ttttacaggt gatatcatca aatatgatca gcaaggccag caaattatcg
     8401 tgaaaaattt tgccaaaaat gtgagccgga ttatccgtat aagcgatatt caacgcctgc
     8461 gatttgtccc ctcaactgtc caaacagccc aaaaaaatag atttaagaaa gagtgagatg
     8521 tagttgcttc atcccactct tttttcttag cgaatttgtt caaaatgtaa atgaactgca
     8581 atatgatctc cataaccact tctttccaag tcacgttgta aacgatagga aatgtagtgt
     8641 tctgcaatgg taatgtaacc tgcgcccaat aaacgatgtt caaccataga ttgaatcata
     8701 ctgatagtcg cacgttccac cttggcttct tgtaaatcca aaactacctt cttagtgact
     8761 tgagcaagat tttgacgcaa atcatctgtc aaaacataaa cagtttgggc tgccttcaag
     8821 atggcttggt aaatcttatc tggattaaat tcagcaattt cgccattacg tttgattact
     8881 tgcataggtt tctcctttat tctttgtttt ctttgatttc tgccagcatt ttttcttctt
     8941 ctactgtcag ttgataatgt tcaagtaaat ccggtctgcg ctcgtaggtt ttctttaaac
     9001 tctcgtacaa tcgccactga cgaatctttt catggtggcc actcatcaat acatctggca
     9061 cgaccatgcc tcgataatca tagggacgtg tgtactgagg atattctaaa agacctgaag
     9121 aaaaactatc atcttggtgg ctagactcct tgccaatcac ttctggaatc aggcgaactg
     9181 tagcatcaat catggtcata gctgccaatt ctccaccagt gaggacatag tcgcctaggg
     9241 aaatctcatc tgttaccaag gtcttaatgc gctcatcata accctcatag tgcccacaga
     9301 taaagattag ctcttcctct tgagccaaat cttcagcata agcctgatca aactgctttc
     9361 cagcaggatc gaggagaata acgcgcggat ttttcttttc aatagcatca aaggaatcga
     9421 aaataggttg tgctctgagc aacatgccct gaccgcctcc gtagggctca tcatctacat
     9481 gacgggcctt ttcagcattt tctcgaaaat tatgatactg gatatccaag agcccttttt
     9541 ctcgagcctt tccaacgatt gagtgctcca gtggagaaaa catctctgga aagagggtta
     9601 aaatatcaat cttcatcgtc taacccttct aagatttcca catcgacccg tttatttgga
     9661 atatcaacat tgagaaccac tggtgggata taaggtaaaa gcaaatcacg tttgcctttt
     9721 cgtttgacca cccagacatc attagcacct ggttgcagga tttccttgat ggttccaacc
     9781 aagctatcac cctcatagac ttccaaaccg ataatctcgt gatagtaaaa ttcaccatcg
     9841 tctaggtcat tcaaatcttc ctcagcgacc ttgagactat atctcttgta cttttcgata
     9901 gtattgatat ggtacatatc tttgaattta ataatgtcaa agttcttctg tttacggtgg
     9961 ctagcgatgg tcactgtttg gacaaactga tctttttcat caaacaaagc cagctcagct
    10021 ccttttttaa accgttcttc tgcaaaatcc gtcacagaca agactcgcat ctcaccctgt
    10081 aatccctgcg tattaacgat tttcccaaca ttaaagtagt tcatcttgtc tcctgtaatc
    10141 tccttttttc catcttattc tgacaattct cgaataatag ccgcaatttt ttccgattct
    10201 gaccattgta aataatggtg attccctcct aaaatgagtt tagtattgga agtccaatat
    10261 tctgattctc tgtactcttt ttctctataa ggctgacaaa aaacaaatac aggaatatga
    10321 gcttctatag atacatcctc aaaatcttcc tcagtaatct ctccagatat ctgaaattct
    10381 ggatcttgat tttccaactc taagcctttt tcttgcatta attcccagat ttttttattc
    10441 gtttcaggac taaatgttgc ttgagttaag ttcttaaaat aaagttcagg accacactcg
    10501 tcaatcagcc tcatctgctc ttccatttct ggataaggat tttctgaaaa atcagcaaac
    10561 atgacttttt tagttgtcgg ttcaattgct actaaagtct gacgcttaat tggtttctcg
    10621 agtaatttgc aagctaaaat tccactccaa ctatgtgcac aaagtatata ttcagaaatt
    10681 cctaattctt caagtacttc ataaaccgca tctgcaagat tatctagatt ttttccagct
    10741 tggtcatgaa tcggactcct acctgtgttc ggaaaatcaa ttgtcaaata accaattgta
    10801 ggaggaggtt tttcaagtat aagtgaaaaa ttttcataac ttggtagcaa acctgctccg
    10861 tttagacaaa ctagcacttt cttttgcttt tgataagtaa cagagaggct accaatttct
    10921 gtagatactt caaacctctt cataaagaaa tccactcatt ctatataatg aattattaaa
    10981 aatccttatc ttttatttta tcacgttcca aggattttct caagttggag gaaggggaca
    11041 acatctctac tttcccttca aaaatccttc caaatcatgt ttatgttggt aattaacggc
    11101 tgcggttttg tctttctcaa agacagtctt ggtaaggtca atatgattaa tagctacgat
    11161 tgcgacggtg tagtaaatga tatcagccag ttctctggca agttcctcgt tcgaatccta
    11221 tcccttcttt tcgaccagag cgcctattca aaacctcggc tacttctccg acttcctcca
    11281 ctaacttcat aaagagacct tcatcagtcc gagactgctg ttaatgttcg attaagtagt
    11341 cttggaattg cctaaacgtt caatctttta tagtatattg aaactagaat agtacacctt
    11401 tacttctaaa acattgttag aaatcgattt gactgtcctg atcgatttgt cctgttcttg
    11461 tttcatttta ctatatcttc tattccacac aaaaaagcga gacatccgtc ccgcccttct
    11521 tatttttcgt caataacgat tcttactttt ttgtattcag ttgggacaga gtagacaatc
    11581 gttcttatcg cagaaatagt gcgaccctta cgaccgatta cacgacccac atcgctttga
    11641 tcaagattca aatgatattc caaaaattct ggtgtatcct caatcttgat agttaaggca
    11701 tctggttgtg aaattaaggg tttcacaatc gcaataatga gattttcaat cgtatccatc
    11761 tgtcaaccta ctttaaactt attttgaaaa tttagaatcg tggaattttt tcaatacgcc
    11821 ttcttttgaa aggatgttac gtactgtgtc tgaaggttga gctccattag ccaaccatgc
    11881 aagaacgcgg tcttctttca aagttacttg gttttcagca acaagtgggt tgtaagttcc
    11941 aactgtttcg atgaaacgtc cgtcacgtgg tgaacgtgaa tctgctacgt tgatacggta
    12001 gaaaggtttt ttcttagaac ccatacgagt caaacggatt ttaactgcca tttttaaagt
    12061 ctcatttctt taatttttta tttcggtgaa atagctgagc tatttagcac atgttctatt
    12121 atagcagatt tctggcatgt gtcaagaaaa aaacttgaca gactataaat ttcttaaagt
    12181 atttagaaat ttgttagaat aggaaataaa tgtttgaaag atgctatccg tgaatactat
    12241 tttagaaaaa ttttataaag aacaccaagt caaacccttc atatcgcctg aacgagagtt
    12301 ggatacttgg cttctaagtc ccaagcccgt tcccaagcga aatatggact tattagtaga
    12361 tgattcacta gcaggagata tcattttact atggagaatc cagtttggta cttttaccac
    12421 tgaaacatga ttttaaaaac tcccattcga atatgattca aaattcgctt agaatcaatg
    12481 ttttcccgct ttttcaaaaa ataaattttc atctaattta aataactttc aattaagtgt
    12541 gaattttgct taaaatcatt aaaaagcacc ccatggtgtg aaccatgaag tgttgtaaat
    12601 gctgtgttgt agctagttct tagcgtttag agaactggct agctttacga gctttcttaa
    12661 gatctggttt ccaaaatact ttgagtacac ttatggtatt gatgccccta aacatctaaa
    12721 aactctggta gaaaaagggt atgctcttgt cgaaacttct tttgactcac ttgaccatct
    12781 aaataatacc atgaaaaaga atatcctgaa gaagaaggga gttactggac tttctaaaat
    12841 gaaatcttgc gacctggatc aagcccttca tgagcatttt tcagaagaag aattagctgg
    12901 tcactttcat gtccttctat ggactttttt acaatggcat tgctatcaca cccaatacct
    12961 atctaagcgc ctggttcgta aactttattg cagctcttcc tctaaatttc ctaattgttg
    13021 aaccaattgc ccgttttata ctaagttctt ttcagaaacc atttactggg gaagaagttg
    13081 aagaagttga agattttcaa gatgatgatg aaatcccaac tattatctaa gccagttctg
    13141 taaactacta atatttgaaa tccacttctt tttagggtac aatggtataa atgaattttt
    13201 gagaggatca gaatgaaaaa actagcaacc cttcttttac tgtctactgt agccctagct
    13261 gggtgtagca gcgtccaacg cagtctgcgt ggtgatgatt atgttgattc cagtcttgct
    13321 gctgaagaaa gttccaaagt agctgcccaa tctgccaagg agttaaacga tgctttaaca
    13381 aacgaaaacg ccaatttccc acaactatct aaggaagttg ctgaagatga agccgaagtg
    13441 attctccaca caagccaagg tgatattcgc attaaactct tccctaaact cgctcctcta
    13501 gcggttgaaa atttcctcac tcacgccaaa gaaggctact ataacggtat taccttccac
    13561 cgtgtcatcg atggctttat ggtccaaact ggagatccaa aaggggacgg tacaggtggt
    13621 cagtccatct ggcatgacaa ggataagact aaagacaaag gaactggttt caagaacgag
    13681 attactcctt atttgtataa catccgtggt gctcttgcta tggctaatac tggtcaacca
    13741 aacaccaatg gcagccagtt cttcatcaac caaagctcta cagatacctc ttctaaactc
    13801 cctacaagca agtatccaca gaaaattatc gaagcctaca aagaaggtgg aaaccctagt
    13861 ctagatggca aacacccagt ctttggtcaa gtgattgacg gtatggatgt tgtggataag
    13921 attgctaagg ccgaaaaaga tgaaaaagac aagccaacta ctgctatcac aatcgacagc
    13981 atcgaagtgg tgaaagacta cgattttaaa tcttaaaaac caaaaaaata cagtatccac
    14041 attcggtact gtatttcttt tactctcatt cttaagttaa attattaaaa tcccatattt
    14101 ggtctatcca gccttcataa aagtctggct cgtggcagac cataaggata gatcccctat
    14161 attctttgag agcgcgtttg agctcatcct ttgcatccac atccaaatgg ttggtcggct
    14221 cgtccagcac taaaacgttg ttttcacgat tcatcaagag acagaaacga accttggctt
    14281 gctctccccc tgataatact tgaatctggc tttcaatatg tttggttgtc aaaccacaac
    14341 gggcaagggc tgcacggact tctgcttgat taagggcagg aaaggcattc cagacagctt
    14401 caagaggagt ttggcgatta ccgccttcta cttcctgctc aaaataacca agttctaaat
    14461 aatctccacg ctccacttcc ccagcgattg gcgagataat gcccaagaga ctcttcaaga
    14521 gagttgtttt tccaatacca ttagcaccaa taatcgcaac cttttgattg cgttcgaagg
    14581 taagatttaa aggcttagta agaggaaggt cgtaaccaat ttgcaagttc ttggcttgga
    14641 agataaagcg ccctggtgta cgagctggtt tgaaatcaaa ggatggtttt ggtttctcac
    14701 tttggagttc gataatatcc atcttatcca atttcttttg acgagacata gccatattac
    14761 gagttgcaac acgggcttta ttacgagcca caaagtcctt gaggtctgca atctctttct
    14821 gctggcgttc gtaggctgcc tctagctgag atttcttcat agcataaact tcttggaact
    14881 ggtagtagtc accagagtaa cgcgtcagct gttgattttc cacatgatag acaatattga
    14941 taacgtcatt gaggaatgga atatcgtgcg aaatgagaac aaaggcattc tcatagtttt
    15001 ggagatagcg cttgagccaa tcaatatgct cagcatccaa gtagttggtc ggctcgtcca
    15061 acagcaagat atcaggcttt tcaaggagaa gttttgccaa aagcaccttg gttctttgcc
    15121 cacctgacaa agaagttaca tccgtatcca tgccaaagtc cataacacca agagcacgcg
    15181 ctacttcgtc aatcttagca tccaaggtat agaaatcacg actctccaga cggtcttgaa
    15241 gttctcctac ttcttccatg agagcatcaa catccgcacc gtcttcagcc atttccatat
    15301 agaggtcatt gatacgagct tcagctttga aaagctcatc aaaagccgta cggagaacat
    15361 cacgcaccga ctgtctttca gcaaggacag agtgctgatc caagtaacca gccgtcacat
    15421 atttggacca ctcaaccttt ccttcatctg gcagcatttt accagtcacg atactcataa
    15481 aggttgattt tccttcacca ttggcaccga ccaggccgat atgttctccc ttgaggagac
    15541 ggaaggacac atcttcaaaa attgcacggt caccaaaacc gtgactcaga tttttaactt
    15601 ctaaaatact cattttaatt ccttatcttg tttttatgta atcgtttata aaggagccaa
    15661 gccagatagc cacccaaagt gttggtccac aaatcatcaa tctcaaagac gcgattgaaa
    15721 tcaaagaaaa agtccaagat taattgcgta cactcgattc caagactcac aagaaaacta
    15781 aaaagaagga ccttttttgt tttccgcaaa tttggaaata gataaaggag ttggaaaatc
    15841 agaggaaaaa acaagaagac attgaggata ttttgtaaaa aaatccaaca taattgtcca
    15901 atgtcactca cttcgcccag tttccagaga gaattgaaag gagtcaaaag aaaaaccagg
    15961 cgtccaagat gctgaatatc tggagttccc actcccacgg tagattgttc ttgaggagta
    16021 aagcaaaaat agacaatgca aatgctatag aaaatgactc cccagaccaa aatatgatta
    16081 taagtcttct tcatcattaa ggatttaccg ctgcgactgc cttctggcgg tcacgtttca
    16141 ttgtgttaaa gagcaattgt ccacaagctg cgtcaatatc tgtaccatgc tcttgacgaa
    16201 ccacacagtt gccccctttt ttcttaagcg tatcatagaa agccaacacg cgctctttgg
    16261 gactacggct atattggtca tgctcactaa ctgggttata aggaatcaag tttacataag
    16321 acgatttctt gatgttcttg agcaattcag ccaattccaa ggcttgttcc acaccgtcgt
    16381 tgacttcatt aagcatgata tattcaaagg ttacacgacg gtttgttgtc tcaatgtagt
    16441 attcaatagc agcaaagagt ttt
//
LOCUS       DAVBCR010000043        16141 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCR010000043 DAVBCR010000000
VERSION     DAVBCR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.043"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            145..6840
                     /locus_tag="VQ508_001322"
     CDS             145..6840
                     /locus_tag="VQ508_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2490315.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASEAS
                     QTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7088..7944)
                     /locus_tag="VQ508_001323"
                     /pseudo
     CDS             complement(7088..7944)
                     /locus_tag="VQ508_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8155..8514)
                     /locus_tag="VQ508_001324"
     CDS             complement(8155..8514)
                     /locus_tag="VQ508_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2490316.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8606..9562
                     /locus_tag="VQ508_001325"
                     /pseudo
     CDS             8606..9562
                     /locus_tag="VQ508_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9546..10103
                     /locus_tag="VQ508_001326"
     CDS             9546..10103
                     /locus_tag="VQ508_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2490317.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10096..10350
                     /locus_tag="VQ508_001327"
     CDS             10096..10350
                     /locus_tag="VQ508_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490318.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10362..12416
                     /locus_tag="VQ508_001328"
     CDS             10362..12416
                     /locus_tag="VQ508_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2490319.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12595..13455
                     /locus_tag="VQ508_001329"
     CDS             12595..13455
                     /locus_tag="VQ508_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2490320.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13666..14376
                     /gene="ccdA2"
                     /locus_tag="VQ508_001330"
     CDS             13666..14376
                     /gene="ccdA2"
                     /locus_tag="VQ508_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2490321.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14396..14962
                     /locus_tag="VQ508_001331"
     CDS             14396..14962
                     /locus_tag="VQ508_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2490322.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14973..16085
                     /gene="msrB"
                     /locus_tag="VQ508_001332"
     CDS             14973..16085
                     /gene="msrB"
                     /locus_tag="VQ508_001332"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2490323.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4904 a         3053 c         3568 g         4616 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtgaagc tagtcaaact
     6721 aataaagccc agttaccaaa tacaggtagt gcggcaagcc aagcagcagt agcagcaggt
     6781 ttagctcttc taggtttgag tgcaggatta gtagttacta aaggtaaaaa agaagactag
     6841 aaattttgat accttcttta tcattaaaga ccaaagaaaa actcttgtcc taaatagaag
     6901 ataaagagtc tggaacaaaa tagttctcaa ccacaaaaaa gctagatatt tccaattgcg
     6961 gaactctagc tttttaattt tgagtcgttc tcttattgta tagtagattg aaactagaat
     7021 agtacacttc tacttctaaa acattgttag aaatcaattt gactgtcctt atcgatttgt
     7081 cctgttctta tttcatttca ctatgtttta ggaggttatt ggccataagt accaatccca
     7141 tgtcaattct cacttgacgc ttccctctca gattacatct cttgtaaccc aaacaagcct
     7201 ttatctgccc aaagacaggt tccacatcaa tcttgcgttg agcgaaaatc tgtctacctt
     7261 ggggagataa aagcgcctga cattctttag ctttcaagtt ttgatagcgt tcgttcatat
     7321 acagtccctt ttgagggact gattcaggtt catcagcgta gtaaaccttg atttcctgtt
     7381 gaaagtctgt ctgtgttttc tggtacttga tatggtgaaa acgatagcac cagccatcag
     7441 gatgtgtgta gctatcctcc ttgtcattat agtgccaatt cgctaagttt ttagctgact
     7501 gtttataccc tctcttttgt tccttatcaa acatggcata tttaatcaga aggggagtga
     7561 tttcttcttg aaaatagacc tgtatctgtt cttgaagttt ggcggagaat ttctctgtcg
     7621 ttttcttcca cacaaaacta tacttgttgg cattggcttc aatcttagtt ccgtcaataa
     7681 acaaacaatc taaggtcact aactcttcca tttttcaatt tttcgaccag agaaaatccc
     7741 ttgtgaatag gcaaatagaa gagtagatac aagcatttta gggtgataag acgggcgatc
     7801 aaaggcatga tagaaggcgt ggaagtgaca atcctccaag gtattcacca ctttttcaat
     7861 agtaaaaacg agatgatctt gtggcaagaa ggaactgatt tctagtggta aagttgtttg
     7921 atttgtgtta tagtgaatat gcatgggata gcctttctaa atggtttatt aagcaattct
     7981 attttaccaa gactatcgca accatgcaaa aaagcaacgg caaatccgtt gccttttttg
     8041 gagataagag actattgtcc cagactcttt tctttgtata aaattgtata gtgttattgt
     8101 ctatactatt ttgtagagaa agcaaaaatc cctagaaaat gcttagggat tcgactattt
     8161 cattaagaac aattcaacaa tcatttctat atacatgata agggttaata agaatcctgc
     8221 acgccagaag aatttgataa atttaggata gtaaaagctg cgttttttca aaaggaagaa
     8281 aaagacgaga ataatggcta ggatcgagag ggctagcccc agtctaggga ggaaattatg
     8341 ggtaaaggtt ttagctgtaa tcaagtaata ctcaaatacc aagactggaa aagccaaatc
     8401 cgcaaagttt cgtcctagtt tttttaatct aaaaagtttg gttaggataa tgcagacgac
     8461 taaggtcagt atcaataata aaatagatgc taatttcatt aaaatcatac ccatattgta
     8521 tcataaaaaa aggctaatgg aaacgagaaa cagaggaatt tataggaaag tcaggctttt
     8581 gagattgtgg gtgatttttg ttataatgaa agttataaaa tcttataata ccttgaatga
     8641 ttattatcga aaactctttg gagaaaagac ttttaaagtc cctattgatg cgggatttga
     8701 ctgtcccaat cgtgatggaa ctgtggctca tggaggctgt actttttgta cggtttccgg
     8761 ttctggagat gccattgtgg caccagatgc gcctatccgt gagcaatttt ataaggaaat
     8821 tgactttatt catcgtaagt ggccagatgt tcagaagtat ctggtttatt ttcaaaattt
     8881 taccaacacc catgaaaagg tggaagttat tcgagagcgt tatgaacagg ctatcaatga
     8941 gccaggtgtg gtaggaatca atattggaac gcgcccagac tgtttaccag acgaaaccat
     9001 cgaatatttg gctgagttat cggagtgcat gcatgtgacg gttgaattgg gcttgcaaac
     9061 tacttatgaa gcaacctctg acctgattaa ccgtgcgcat tcttatgaat tgtaggtgga
     9121 aacggtcaag cgtttgagaa agtatccaaa aattgagatt gtttcccatt tgatcaatgg
     9181 tttgcctggt gaaacccatg agatgatggt tgaaaatgtc cgccgttgtg tcacggataa
     9241 cgatattcaa gggattaaac tgcatttgct ccatcttatg acaaatactc gtatgcaacg
     9301 agattaccat gaggggcgtt tacaattgat gagtcaggac gaatatgtca gggtcatctg
     9361 tgaccaactg gaaattattc ccaaacatat cgtcatccat cgaattacag gagatgcgcc
     9421 tagggatatg ctgcttgggc ctatgtggag cctcaataaa tgggaagctc tcaacagcat
     9481 tgagatggag atgcgacgtc gtggaagtgt tcaaggatgc aaggctgtaa aacaggagtt
     9541 tgaaaatgaa aagaccactt gagatggcac atgatttttt ggctgaggtc gtgacaaaag
     9601 aggatgtcgt agtggatgcg actatgggaa atggtcatga cacgcttttt ttagccaagc
     9661 tagccaagca agtctatgcc tttgatattc agaagcaagc cttggaaaag acccaagagc
     9721 gtttgcatca ggctgacttg acaaatgccc agttaatctt gcaaggccat gagacactgg
     9781 accagtttgt gataaaagct aaggcaggga tttttaatct gggctatttg ccgtcagctg
     9841 ataagtctgt catcacccga ccgcagacaa cgattgaggc attagaaaag ctatgtggct
     9901 tacttgtcaa aggtggacga attgctatta tgatttacta tggtcatgaa ggaggcgatc
     9961 tcgagagaga tgctgtcttg gattttgtga tccagttgaa ccaacaagag tacacagctg
    10021 ccatttaccg aactttaaac caagtcaaca acccgccgtt tttagtgatg attgaaaaat
    10081 tagagagata cagacatgga taaacaatac ctacacgaaa agctggatgc catgcgccag
    10141 aattttgttg aatcaaccca ccacgaacga tcggtgggcg tgctagacca agcgcatatg
    10201 agcaaaaaaa tgcttaaaat caagaaaaaa ttagttgctc ttgaaatgga acggtgccag
    10261 agaaaaattg agcacaaaga ctgttccaag attgaccaaa aaatcaaaga gcagaaggag
    10321 atatttgaat cctgttgtaa aaaagattaa ggaggacgtg cgtggaatta ctgatttacc
    10381 tcatcctatt tttactggtt ttgattgtct caagtacaac caataagctc ctgccctttt
    10441 tgcctctccc tttggtgcag attcttttgg gaattgtgat tggtctcttt ttacccaata
    10501 ctgactttca ccttaatacg gagttgtttt tggcactggt tatcggaccc ttgcttttcc
    10561 gagaggctga agaagcagat gttacggcta ttttaaaaca ctggcgaatc attgtttatc
    10621 tcatatttcc agtgattttt atctcgacct tgagtttggg tggcttggcc catcttcttt
    10681 ggttcagcct tcccttggca gcttgcttgg ctgttggggc agcccttggt cctacggact
    10741 tggtggcctt tgcctctctt tcggagcgtt ttagctttcc taagcgcgtg tccaatattc
    10801 ttaagggcga aggactcttg aatgatgctt ctggtttggt ggcttttcag gtagctttga
    10861 cagcttggac aactggagct ttttctctgg ggcaagctag cagttcgctc atcttttcaa
    10921 tcctaggcgg ttttttaatt ggatttttaa cggccatgac caaccgcttc ctccatacct
    10981 tcttgctaag tgtgcgcgca acggatattg ccagtgaact tttattagaa ttgagtttgc
    11041 ctctagtgac cttctttctg gcagaagaag ttcatgtttc aggtattatt gccgtcgtag
    11101 ttgctggaat tttaaaggca agtcgcttca agaaaatcac gctcctcgaa gcccaagtgg
    11161 atacggtgac cgagacggtc tggcatacag tgacctttat gctcaacggt tctgtctttg
    11221 tgattttagg gatggagttg gaaatgatag cagaacctat cttgaccaat ccaatctata
    11281 atcctctact tttattgcta tctctcatcg cccttacctt tgtcctcttt gtcattcgtt
    11341 ttattatgat ctatggctat tatgcctata gaacccgacg cctcaagaaa aagctaaata
    11401 agtatatgaa ggacatgttt ctcttgacct tttcaggtgt taagggaacg gtgtcgattg
    11461 ctacgattct cttgatacca agtaatctag aacaggagta tcctcttttg cttttccttg
    11521 ttgcaggtgt gacgcttgtc agctttttaa caggtctctt ggtcttgcct catctttctg
    11581 atgaagagga agaaagcaag gattatctca tgcatatcgc cattttgaat gaagtaacgc
    11641 tagagttgga aaaagagttg gaagacacca gaaataaact tcccctctat gcggctattg
    11701 acaattatca tggacgtatt gaaaatctca ttttaagcca agaaaaccag gatgatcaag
    11761 aagattgggc tgctttgaaa ctcttgattc ttagtattga aagtgatggt ttggaacagg
    11821 cctatgaaga ggggaacatt agcaatcgtg cttaccgagt ttaccaacgt tatctgaaaa
    11881 atatagaaca aggaatcaat cgtaaacttg cctcaagact gacctattat tttcttgttt
    11941 ccttgaggat tttacgtttt ctccttcatg aagtttttac tcttggaaag accttccgta
    12001 gctggaagga caaggagcaa agccgtctcc gtgctcttga ttatgaccaa attgcagagc
    12061 tctatcttgc caatacagag atgattattg aaagtttgga aaacctgaag ggagtctaca
    12121 gacgctcttt gattagtttt atgcaggagt ctcgtcttcg agaaacagct attatcagca
    12181 gtggtgcctt tgtcgaacgg gttatcaatc gtgtcaaacc caacaatatc gatgaaatgc
    12241 tgagaggcta ttatctggag cgcaagttga ttttcgaata cgaagaaaaa cgattgatta
    12301 cgactaagta tgccaagaaa ttacgacaaa atgtaaataa cttagagaac tattccttga
    12361 aggaagctgc caataccctg ccgtatgata tggtggaatt ggtaagaaga aattagttaa
    12421 tactcttcga aaatctcttc aaaccacgtc agcgtcgcct tggattatat atgtgactga
    12481 cttcgtcagt ttcatctaca acctcaaagc agggctttga gcaacctgcg gctagcttcc
    12541 tagtttgctc tttgattttc attgagtata agattataaa tgaaggagta tgacatgaaa
    12601 aagtggtgga aagagctgat agacaagcct ttattaaaag cttttttgca ttattatcaa
    12661 gcatcagata gtgagttgac cagtgtcgca gtagcctact attggttgat ttcgattttc
    12721 cccctgcttt tggtggtggt caatatcctc ccctattttc agattcctgt gggagaattt
    12781 ctgtgcttta tgaaagacgt tctgccccca agcctctatg aaggtgtgga aaaaatagcc
    12841 agagaagttt tgacgcaacc ttcaacaggt ttattgagtt tttctgtttt atcggcgtta
    12901 tggagttttt caaaatctat gaatttcttg caaaaagctt ttaacaaggc ttatggcgtc
    12961 gaaaagagtc gtggacttat ttcccatcag atgctaagtc tcctagtcag ttttggcttg
    13021 cagctcctct ttgcgtttgc cttgtttttg atcttatttg gtcagatgat tttggcttta
    13081 cttgctcatt attggacaaa agatggcatt atctatcagg ctttacaagg gttggcaggt
    13141 cctctgattt acgccctcct ctttgctatc ttggtcatgc tttattattt tcttcccaat
    13201 ctatctaaca ggaaaattag ctatacccta ccaggcagtg cctttgttct cttagtgatt
    13261 ttaggtttac taactctgtt ctctagttat ctcaattact atgtccatca tttggtggat
    13321 gtccgaattt taggttccgt tcttcttgtt gttatgatgt tttggtttat cttgattgct
    13381 aaaattgtta tcctaggcgc ggttatcaat gccagtatgc agagtttgaa agatccggtc
    13441 tttaaaaaag actaactatt tatccattac aaaagcgcat agtatcaaaa ctttatagaa
    13501 ccttgatact atgcgctttt gtgctttgac agttttttgt atagtttttg tccaagattt
    13561 ctaaatcatc tgattaaatt cctattttcc cctatcattg tccctgtttt ttcgaaaatg
    13621 gaaggagtat aataaatcta tcaataaaat gataggagga actttatggg tcatattttc
    13681 ttttttctaa gtgtcttttt ggcagggatt ctatccttct tttctccttg tatcttacct
    13741 ttgttaccgg tctatacagg agtgttacta gatgataagg atggtgctca ggcttctagc
    13801 ggcaaatttt caatctcagt tactagttta ttacgaactc tggcctttat agcaggaatt
    13861 tcctttatat ttattttgtt gggctatgga gctggttttt taggcgattt gctttatgct
    13921 tcttggttcc aatatcttac tggggcaatt attatccttc ttggtttgca ccaaatggag
    13981 attctacact ttaaggggct ttataaggaa aagaggctac aactgcaagg acaggggcaa
    14041 aatggtaagg gctatagtca ggcattttta ttgggcttga cctttagttt tgcttggacg
    14101 ccttgcgtgg ggccggttct ggggtctgtt ttggccttgg cggcttcagg tggttcagga
    14161 gcttggcagg gagctggtct catgttggtg tatacgctgg gcttggcgct accattcttg
    14221 cttctagctc tgacctctag ttatgttttg aaacatttcc gaaaacttca tccctatctc
    14281 ggaatcctca aaaaagtggg tggttttctc attattgtga tgggactctt ggttctgttt
    14341 ggaaatgctt caattttaag tcaattattt gaataaaatg gaaaggaata tcaatatgaa
    14401 aaaatggcaa acatgtgttc ttggagcagg ttcgctcctt tgtttgacgg cttgttcagg
    14461 caagtccgtg actagtgaac accaaacgaa agatgaaatg aagacggagc agacagctag
    14521 taaaacaagc gcagctaaag ggaaagaggt ggctgatttt gaattgatgg gagtagatgg
    14581 caagacctac cgtttatctg attacaaggg caagaaagtc tatctcaaat tctgggcttc
    14641 ttggtgttcc atctgtctgg ctagtcttcc agatacggat gagattgcta aagaagctgg
    14701 tgatgactat gtggtcttga cagtagtgtc accaggacat aagggagagc aatctgaagc
    14761 ggactttaag aattggtata agggattgga ttataaaaat ctcccagtcc tagttgaccc
    14821 atcaggcaaa cttttggaaa cttatggtgt ccgttcttac ccaacccaag cctttataga
    14881 caaagaaggc aagctggtca aaacacatcc aggattcatg gaaaaagatg caattttgca
    14941 aactttgaag gaattagcct aggaggtgtc ttatgaatga taagttaaaa atcttcttgt
    15001 tgctaggagt attttttcta gccataaccg gtttctatgt tctattgata cgaaatgcag
    15061 ggcagacaga tgcctcgcaa attgaaaagg cggcagttag ccaaggagga aaagcagtga
    15121 aaaaaacaga aattagtaaa gacgcagact tgcacgaaat ttatctagct ggaggttgtt
    15181 tctggggagt ggaggaatat ttctcacgcg ttcccggggt gacggatgcc gtttcaggct
    15241 atgcaaatgg tagaggagaa acaaccaagt acgaattgat taaccaaaca ggtcatgcag
    15301 aaaccgtcca tgtcacctat gatgccaagc aaatttctct caaggaaatc ctgcttcact
    15361 atttccgcat tatcaatcca accagcaaaa ataaacaagg aaatgatgtg gggacccagt
    15421 accgtactgg tgtttattac acagatgaca aggatttgga agtgattaac caagtctttg
    15481 atgaggtggc taagaaatac gatcaacctc tagcagttga aaaggaaaac ttgaagaatt
    15541 ttgtggtggc tgaggattac catcaagact atctcaagaa aaatccaaat ggctactgcc
    15601 atatcaatgt taatcaggcg gcctatcctg tcattgatgc cagcaaatat ccaaaaccaa
    15661 gtgatgagga attgaaaaag accctgtcac ctgaggagta tgcagttacc caggaaaatc
    15721 aaacagaacg agctttctca aaccgttact gggataaatt tgaatccggt atctatgtgg
    15781 atatagcaac tggggaacct ctcttttcat caaaagacaa atttgagtct ggttgtggct
    15841 ggcctagttt tacccaaccc atcagtccag atgttgtcac ctacaaggaa gataagtcct
    15901 acaatatgac gcgtatggaa gtgcggagcc gagtaggaga ttctcacctt gggcatgtct
    15961 ttacggatgg tccacaggac aagggcggct tacgttactg tatcaatagc ctctctatcc
    16021 gctttattcc caaagaccaa atggaagaaa aaggctacgc ttatttacta gattatgttg
    16081 attaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tatgtagggt
    16141 t
//
LOCUS       DAVBCR010000044        16078 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCR010000044 DAVBCR010000000
VERSION     DAVBCR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.044"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(105..1241)
                     /gene="lctO"
                     /locus_tag="VQ508_001333"
     CDS             complement(105..1241)
                     /gene="lctO"
                     /locus_tag="VQ508_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2490324.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1866..3356)
                     /gene="lysS"
                     /locus_tag="VQ508_001334"
     CDS             complement(1866..3356)
                     /gene="lysS"
                     /locus_tag="VQ508_001334"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2490325.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3484..3630)
                     /locus_tag="VQ508_001335"
     CDS             complement(3484..3630)
                     /locus_tag="VQ508_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2490326.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            3856..4524
                     /locus_tag="VQ508_001336"
     CDS             3856..4524
                     /locus_tag="VQ508_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2490327.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            4484..5161
                     /locus_tag="VQ508_001337"
     CDS             4484..5161
                     /locus_tag="VQ508_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2490328.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            5162..5919
                     /locus_tag="VQ508_001338"
                     /pseudo
     CDS             5162..5919
                     /locus_tag="VQ508_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5931..6792
                     /locus_tag="VQ508_001339"
                     /pseudo
     CDS             5931..6792
                     /locus_tag="VQ508_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(6872..7519)
                     /locus_tag="VQ508_001340"
     CDS             complement(6872..7519)
                     /locus_tag="VQ508_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490329.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7516..8214)
                     /locus_tag="VQ508_001341"
     CDS             complement(7516..8214)
                     /locus_tag="VQ508_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490330.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(8224..8976)
                     /locus_tag="VQ508_001342"
     CDS             complement(8224..8976)
                     /locus_tag="VQ508_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490331.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(8976..9740)
                     /locus_tag="VQ508_001343"
     CDS             complement(8976..9740)
                     /locus_tag="VQ508_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490332.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(9836..10239)
                     /locus_tag="VQ508_001344"
                     /pseudo
     CDS             complement(9836..10239)
                     /locus_tag="VQ508_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10419..11051)
                     /locus_tag="VQ508_001345"
     CDS             complement(10419..11051)
                     /locus_tag="VQ508_001345"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2490333.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(11085..11786)
                     /gene="pyrF"
                     /locus_tag="VQ508_001346"
     CDS             complement(11085..11786)
                     /gene="pyrF"
                     /locus_tag="VQ508_001346"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2490334.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            12107..12199
                     /locus_tag="VQ508_001347"
                     /pseudo
     CDS             12107..12199
                     /locus_tag="VQ508_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12521..13234)
                     /locus_tag="VQ508_001348"
     CDS             complement(12521..13234)
                     /locus_tag="VQ508_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490335.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(13227..13922)
                     /locus_tag="VQ508_001349"
     CDS             complement(13227..13922)
                     /locus_tag="VQ508_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490336.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(14262..15594)
                     /locus_tag="VQ508_001350"
                     /pseudo
     CDS             complement(14262..15594)
                     /locus_tag="VQ508_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
BASE COUNT         5235 a         3266 c         2650 g         4927 t
ORIGIN      
        1 gatttgtcct attcttattt cattttacta tagttttcga tagcaattta ttcttccaat
       61 acacgaagaa aaacctccac attcagtgga ggcaatctgt tttatcaata caattttaag
      121 tcacgagggt caactgggaa ggttgggttg tatggattgt gacggagctt aaagtgtttg
      181 acatcttcaa tggtctgagc tccagacaat tgcataactg tcttcaattc cgcattcaag
      241 tgttcaaaga cttgacgcac accgacacta ccaccgagag ccaagccata aataacaggg
      301 cgaccaatag ctaccaagtc tgctcctgaa gccaaggctt taaagacgtg ttgaccacga
      361 cgaacaccag agtcaaagac aatcggcaca cgtctatcaa ctgcttctgc cacttcttga
      421 agcgagtcaa aggcagctgg tccaccgtcg atttgacgac caccgtggtt ggttacccag
      481 ataccagaag ctcctgcagc aagtgaacgt tcaacgtcct cacggcattg tggtcccttg
      541 acatacacag gaagaccaga gtattcagcg ataaattcta catcgcgtgg agacaagcgt
      601 tgtttagctg atttgtaaac aaagtccatt gatttaccag caccttctgg caggtattct
      661 tcaacaatcg gcatgccaac tgggaagaca aaaccattac gcttatccac ttcacgattc
      721 ccccctacag tagcatctgc cgtcaagaca atcgctttat aaccttcagc cttcacacgg
      781 tccatgatgt ggcggttgat accgtcatcc ttactaaagt aaaattggaa ccaatggggt
      841 gtcccttgaa gggcttcaga aatctctgga aggtcaacag tagagtaaga gctggttgta
      901 taaagagaac caaactcatg cacaccacgc gcagtcgcca cttccccctg ttcatttgcc
      961 aatttatgag ccgcaacagg cgccataatg attggtgaag atagtttttc acctgcaaat
     1021 tcaatctctg tacttggatt ttcaacatcg caaagtgtat gaggaacgat gagtttgtgg
     1081 ttaaaggcgc ggatattctc tcttaaagtg aaagtatctt ccgccccact agcgatatag
     1141 ccaaatgctg ctttaggaat aacttgttgc gccattggct ccaaatcata ggtattgatg
     1201 aagtctacat gaccttctgc attgcttgtt ttgtatgaca taaaatgccc tccttgatta
     1261 agtaagcgtt tgctttttac tacacaacta taataactct tttttcaatc attttcaaga
     1321 gttttgctta aaaaccatct tattttttca tacaatatac tagccctact ttccttaaac
     1381 tctaaacttt agtctatatt aagtgaatcg ttgagatttt ttgctgccag gcatttgaac
     1441 tactttttta tttgcggatt ctatctacta atactataaa aaaacaagtg aataagacac
     1501 cagaacatcc tctaatagga cctatggact tatatcagtc ccatatatac tgtttaagca
     1561 agatactatc ctagttgacg ctaacttcct atcccactta ttaatttttt atttatagtg
     1621 aatctgaaca aatcaattgg ggaatccaag tcaatttcta gtactttttg aacagtttca
     1681 gcgtactatc gtaaaatgaa ataaaacatg cacaaattgc ttagggaatt taaaccaatt
     1741 tctaacaatg tcttagaaat caaagtgtac tatttcaact tcaatatact acacttcatt
     1801 ttctccttaa attcagtctc tttttgaatc aaaaaatccc ctggtaagag ccagaggata
     1861 agaatttatt tcattgttgg gaagagcaat acatcacgga tagttgttgt atcagtgagg
     1921 agcatgcaga gacggtcgat accgattccc aaaccacctg ttggtggcat accgtattca
     1981 agagcttcaa tgtagtcata gtcgatgcct gtcgcttcat catcaccaag ttctttggct
     2041 ttagcttggg cttcaaaacg gctaagttgg tcgattgggt cgttcaactc agtaaaggca
     2101 ttaccgtact ccttagtcat gataaagagc tcgaaacggt cagtaaagcg ttggtcttca
     2161 ggatttttct tagcgagtgg agatacagct actggatgtc catagacaaa ggttggttgg
     2221 attaaagttt cttcaacaaa ctcttcaaag aaggcattga tgatgtgacc aacctcagtg
     2281 tagtgtttct caactggaac tttcttctca gcagcgatag ttttagcttc ttccaaagtc
     2341 atgtcttgcc agaaatcgac accagtaatt tctctgatag catccaccat atgaacacgc
     2401 ttaaatggtt cgttaatctt gatttcagta ccttggtagt tgactgggcc atcacctttg
     2461 actgatttag cagcgtgttg gataatgcct tcagtcaagt ccatgatgtc ttggaagtct
     2521 gcataagctt ggtaaacttc gatagaagtg aactcagggt tatgagtagc gtccattcct
     2581 tcgttacgga agatacggcc aatttcatag acacgttcca taccacccac gataaggcgt
     2641 ttcaagtgaa gctcagtcgc gatacgaagc accatgtcaa tgttttgggc attgtggtgg
     2701 gtgataaatg gacgggcagc agcaccaccg gcttcattat gaagaacagg tgtttccact
     2761 tcaaggaatc ctttttggtc aaggtaacga cggatttcag agatgatttt tgaacgagtg
     2821 acaaagcgtt caaagctttc acgattagaa atcaagtcaa ggtaacgttt acggtaaatt
     2881 gtttcaacgt ctgtcaaacc atggaatttc tcaggaagag gacgaagagc cttagacaag
     2941 tgtgtgatgt gggttgcctt gatagagagt tctcccatat ccgtacgcat cacttcacct
     3001 tcgacaccaa ggaagtcacc aaggtctgct tttttgaaga tttcgtagtt ttcttcaccg
     3061 acagcatcct tacgaacgta gatctgaatc tggccttcgc ggtcttgaag gtgggcaaaa
     3121 ccaacttttc ctttaccacg tttggttatc aagcgtcctg cgatagtagc tgtttcgttt
     3181 ttatcgtgta attgttcttt atcgaggttg gcatatttat cttttaattc ttgtgaattt
     3241 gcagtacgtt caaaacgttt tccgaaagga tcgattcctt gttcgcggag cgcagccatt
     3301 ttttcacggc gaacgatctg ctggtcattt agttcttcca tatgttctgt agacatggga
     3361 tcctcctagt tttactcaaa attgaatacg atgagtcatt ttttgcgata ctcccatttt
     3421 atcataaata agcaagaaat tcacggataa tctttaaaaa ctaaaaagaa cctgacgcta
     3481 aaatcactgg aaagaagtga aagtatctcc cgccccacta gcgatatagc caaatgctgc
     3541 tttaggaata acttgttgcg ccattggctc caaatcatag gtattgatga aatctacatg
     3601 accttctgca ttgcttgttt tgtatgacat aaaatgtcct ccttaataag taagcgttta
     3661 ctttgtgtat tacaaaaata tcttaactct ttttcaaaac ttttaaaata ttttgtttgg
     3721 aaatttcaga aattttatgt ctatgataaa aatccttata acggcaataa aaaatagata
     3781 ttatccaaag aagattttaa gtgctacaat aactgtatta tttctagatg ggaggttcta
     3841 tttttggatt gatccattgt tgaacaatat ctaccactat atcaaaaggc attctttctg
     3901 accttgcata ttgcagtttg gggaattttg ggatcctttc tgctcggttt aatcgttagt
     3961 atcatccgac attatcgaat ccttgttttg gcgcaagtag cgacagccta cattgaattg
     4021 tcacgtaata cgcccctttt gattcaactc ttctttctct acttcggtct tccccgaatc
     4081 gggattgtcc tatcttcaga agtctgtgca acgcttgggc ttgtcttttt aggaggctcc
     4141 tatatggcag aatctttccg aagtgggctg gaagccatca gtcaaaccca gcaggagatt
     4201 ggcctcgcta ttggtctgac acctctacag gtcttttact atgtggttct tccgcaagca
     4261 acagcggtgg cactcccctc ctttagtgcc aatgtcattt tccttatcaa ggaaacctct
     4321 gttttctcag cagtggcttt ggccgacctc atgtacgtcg ccaaggattt gattggtctc
     4381 tactatgaga cagacattgc gctagctatg ttggtagttg cttatctgat catgctgcta
     4441 cccatctcac tggtctttag ctggatagaa aggaggctcc gccatgcagg attcgggaat
     4501 ccaagtactc tttcaaggaa ataatctcct gagaatctta cagggattgg gcgttacgat
     4561 tgagatatcc atcctgtctg tcctcttatc catgatgttc ggaacagtca tgggaatcat
     4621 catgacctcc cattctagaa tcatacgatt tttaacacga ttgtatctgg actttacccg
     4681 tatcatgccc cagctggtgc tactcttcat cgtttacttt ggcttagctc gaaactttaa
     4741 tatcaatatc tcaggtgaga cttcagctat tatcgttttt accctctggg gagcagctga
     4801 aatgggagac ttggtacgtg gagctatcac ttctctccct aaacatcagt ttgaaagtgg
     4861 acaggcactc ggcttgacta atgttcaact ttactaccac atcatcatcc cacaagtctt
     4921 aagaagactg ctaccgcagg ctatcaatct tgtcactcgg atgattaaaa ccacttcatt
     4981 agttgttttg attggggttg tggaagtgac caaagttgga caacaaatca tcgatagcaa
     5041 tcgcctgacc atcccaactg cttcattttg gatttatgga accattctag tcttatattt
     5101 cgcagtttgc taccctattt ccaaactatc cactcactta gaaaaacatt ggagaaacta
     5161 aatgtctgaa actatcttag aaatcaagga actaaaaaaa tccttcggag acaatcccat
     5221 cctccaagga ctttctctag aaatcaaaaa agggaagttg ttgtcatcct agggccatct
     5281 ggttgtggga aaagtaccct ccttcgttgc ctcaacggct tagaaagtat tcaaggtgga
     5341 gatattcttc tggatggtca gtctatcgtt gaaaataaaa aagattttca cctagttcgc
     5401 caaaagattg gcatggtctt tcaaagttat gaactctttc cccatctgga tgtcttacaa
     5461 aacctcatcc taggccctat caaagctcaa ggaagggaca agaaagaagt aacggaagaa
     5521 gctttgcaat tactagagcg tgtcggtttg ctggataaac aacatagctt tgcccgtcaa
     5581 ttatctggtg gacagaagca acgtgttgca attgtccgtg ccctcctaat gcatccagaa
     5641 atcatccttt ttgacgaggt gactgcttcg ctggatccag aaatggtgcg tgaggtgctg
     5701 gaacttatca atgatttggc ccaagaaggc cgtaccatga ttttagtaac ccacgaaatg
     5761 cagtttgccc aagccattgc tgaccggatt atcttcctcg accaagggaa aatcgctgaa
     5821 gaaggaacag ctcaagcctt ctttaccaat ccgcaaacca aacgagccca ggaattttta
     5881 aacgtctttg actttagcca attcggctca tatctataaa ggagattctt atgaaactat
     5941 tcaaaccact cttaactgtt ttagcacttg cctttgccct tatctttatc actgcttgta
     6001 gctcaggtgg aaacgctggt tcatcctctg gaaaaacaac tgccaaagct cgcactatcg
     6061 atgaaatcaa aaaaaaagcg gtgaactgcg aatcgccgtg tttggagata aaaaaccgtt
     6121 tggctacgtt gacaatgatg gttcttacca aggctacgat attgaactag ggaaccaact
     6181 agctcaagac cttggtgtca aggttaaata catttcagtc gatgctgcca accgtgcgga
     6241 atacttgatt tcaaacaagg tagatattac tcttgctaac tttacagtaa ctgacgaacg
     6301 taagaaacaa gttgattttg cccttccata tatgaaagtt tctctgggtg tcgtatcacc
     6361 taagactggt ctcattacag acgtcaaaca acttgaaggt aaaaccttaa ttgtcacaaa
     6421 aggaacgact gctgagactt attttgaaaa gaatcatcca gaaatcaaac tccaaaaata
     6481 cgaccaatac agtgactctt accaagctct tcttgacgga cgtggagatg ccttttcaac
     6541 tgacaatacg gaagttctag cttgggcgct tgaaaataaa ggatttgaag taggaattac
     6601 ttccctcggt gatcccgata ccattgcggc agcagttcaa aaaggcaacc aagaattgct
     6661 agacttcatc aataaagata ttgaaaaatt agcaaggaaa acttcttcca caaggcctat
     6721 gaaaaaacac ttcacccaac ctacggtgac gctgctaaag cagatgacct ggttgttgaa
     6781 ggtggtcatt aactcttaaa agggactgga ttttaagctc caatcccttt ttaagatttt
     6841 acctataaca tcctgagtct atctaagatg ttcaatctga acacagtgta catactttat
     6901 cttctattgc atatacttta tcacataaga tacgaatatc ctcttcacta tgactagcaa
     6961 tcaaaattgt tgtccctttt tcactagaga gctttctaaa caatgttctc atattttcta
     7021 cacttgattt atccaaggca ttcataggtt catctagtaa aagaatagag ggattctcca
     7081 tgattgcttg agcaatccct agctttttcc tcatacctag cgaataagtt ttaactttct
     7141 ggtctttttg ctcatataga ccaactattt tcagtgtatc attgatttcc tgattaccaa
     7201 ctactcctcg tatgcttgcc aaatattgta aattcttaaa gccactataa taatttataa
     7261 aaccaggttc ttcaatcaaa gctcccaaat tagctggaat ttttctctca ggaacaatat
     7321 tttccccatt gattaacact tctccataag acggactata taaaccagct attaatttaa
     7381 acaatacact tttccctgag ccattcgcac cagtaattcc tataatttcc ccctgtttac
     7441 aactaaagtt aaggttttga aaaacacatg tcttttttaa tttcaactca atatttttta
     7501 atataattat ttcattcatt ctataaacct cctcttttga cgagtgaaat agaaaatgct
     7561 ttgaaaaaga aagactaaaa atagcaactg aagaaacaaa tctcccccta catccccatt
     7621 ccctcgattc aaaatataaa atagataatt agttcgattt cctacaaata gaccaccaaa
     7681 cacaatcatg agtaaaaaga aactaacgca agcaaagttc atttgtacat tcgctcctag
     7741 tgcgtataac ataaagcaaa acaagactaa cacacaagca ttcaaagaat caactataaa
     7801 caaaactcct atatcccagt ttggtaactg taacataaat tgaatcacta gaacttttac
     7861 catgattaaa atgagtccat aaagaaaaca ggtccaaatc tgatacctaa tccagagctt
     7921 aatagaagaa aatcgaatca cttccataaa gaaatcggat gctcttctac gatatacaac
     7981 tgctagaaaa aatgtatgaa taataaacca atgaagcaac catatagata agactaaaaa
     8041 agagttttga ctcagattag gcccttttaa aatttctata acaagtctat catttccttc
     8101 taaccaatac cataatccta aacaaatcaa tatccaaaaa agaacataac cccaaaagta
     8161 cttggtatag attatcttat tcaacctcca catgtgacta cttaactttc ccatttccaa
     8221 cttttacact ctcctccagt ttctatgaac agaaaataca gtcaatggaa ttatgataga
     8281 tagtaatgta agccaataaa tcaatgcata cggattggaa tttaccatat actgaaaaat
     8341 tggcaataat ccaattatcc cattgttcaa ctgataaatc cagaataagg attctacaaa
     8401 taaaaaagta aacagaaata ctaagctcat gtttgatttt ttaaaccaag aaaaactgct
     8461 aatagtaagt aaggataaaa agaaatagta tgctatataa gagaaaaaaa atcctataaa
     8521 gaaactagca ttgtttgcaa tacttatacc ataaaattct cttaaaaaat caacctcctt
     8581 tatctccaaa gagaagctaa aaccattact aaatgcaatt agaaaaatca ataaaaataa
     8641 agtcgccgtc caaatccccg tactaagagc tgctaatttg aaactaaaac tggtaaagtg
     8701 cttaattgat ttcagacgaa tgcgacactc caacctatta aaatagttat tcatcaaata
     8761 aaaaaagaac aatatatatg tgaacggaaa gcaatatact ccagtcgtca tatcttgaag
     8821 taaaactaaa atccattcta atacatttgg atggattgaa tattggcgac agcgcaataa
     8881 atatactgta ctagataaaa cacaggatag cagtaatata aaataaacca atactgataa
     8941 aaaatctttt ttgtaaaatt gaacaaattg tttcattata catagtcctc tgaatgtaga
     9001 aaaaatgtac cataaacaac caaacaacta acaaataaaa taaaagcaag atgcccacta
     9061 actaaggaaa gactgatatc tttctgatat cccaaagcta atgttgtcac aggttctaag
     9121 taagatagcc ctaaaatagc ccaaaaaata ccaccaacca tcatataggc aactgggatg
     9181 aaaatagctc ctattttttt cttcactagc aaagcactag ctagtccaaa aatagagaac
     9241 acagcgcccc aaactccata ccagagagtc gtcacaagac tatagagcaa ctgattagaa
     9301 tcaaataatt cttttaaggc accactataa tctccaatat aaatttcctg ataaggagtc
     9361 actaaaagat taattcctaa taataataaa taggggagaa aaaagactag aaaagaagaa
     9421 ataattgcag tactacctac aatagccaga tacttctttt tagaaattcg gcacaattgt
     9481 gctgttagaa aatgactctc agcatcctct attatctgac tagaataggg cagtgtacag
     9541 ataagtgcag ctactaggct aatcggtgaa aatcctcgaa tagaagaagc ggcaaaaaaa
     9601 atcgataaac cttcaatttt ataaatacca ttgaaagcaa ggaaatttcc taaactcatg
     9661 caaagaaggg tcaaaaataa agacatataa aatcgaggtg attgaacgac tcgtacaaga
     9721 ttacaaatga aaaattccat ccttactcct ccttataata aaaatagggt gtagcattct
     9781 tttttcatgc tacacccaca atcaaccatc tttaaggctt actctgacaa gtaagttaat
     9841 aagaatctgg actccaagaa cctgaagtat gaattcttac atgatttcca aattgtggcg
     9901 ccatagctaa tctagtacca gaaccaatat aattgtcacc acctccatta tagtacatga
     9961 caatcctaga gccagacccc aatgaatata ccggggtaat atctgaccca ctataggcgc
    10021 tacgaataga ggtatttaac cttttaccgc caccagtgct gtcactatta ttaattccag
    10081 cagaggcgtt ttctttctca agctcactag ttgtatccga accccaacgc ggcaacgtta
    10141 aatcataatt tccaatctgt ctagctaaaa ctgtagggag aaacaaacta gatgcaattg
    10201 taactacgca aaaaaggaat atatttttct tcttttcatg acatcgtcct cttaagaagt
    10261 gataaacaag atagttgatt gatcaatttg atacatatta cattttcatt atatcaataa
    10321 aagaaagcgt tgtcaattat tttatttaaa atattgtcat attttaaata aaatggtaaa
    10381 agagactcgg tagcctcgtc tctctttatc ttactgacct aaccttcttg ccaattttct
    10441 tggtcttctt taaagcgttt tagaagatca aggccctctg gcgtgatgta accttcttct
    10501 tgggctagat ggataagctc tctatagttt gaaagcgtca caagtttaac accagcatct
    10561 gcaaagttct tatctgcttt tggcaattgg tagctgaaaa tcgctacaac tccaagtaca
    10621 tctgctcctt ctcgcttggc tgctgctaca gcttcaagaa ctgaaccacc cgttgaaata
    10681 aggtcttcaa ccactaccat tttttgacct tgagctacgc gaccttcgat ttgattacca
    10741 gctccgtggt cttttggttt gctacggatg taggcaaaag gcaagtccat cttatcagca
    10801 ataatggctc cgtgtggaat ccctgctgtt gcagttcctg caatcacttc tacttcagga
    10861 aaggcttctt taatagcttc cacaaaacca ttttcaatta gggtacgagt ttctggatag
    10921 gctagtgtca cacgattatc agtgtaaatc ggtgacttga taccagatgc ccaagtgaag
    10981 ggttcctctg gtttgaggta aacggcttgg attttcaaga ggtggctagc gatatcttta
    11041 gcaagtgtca tggtattctc cttttatttt tctaatctag ttctttaatt ccagttctgt
    11101 gtccattcat ccttgatggc atgataagct gcaacaggat cctcagcttg ggtaatggga
    11161 cgtcccacta cgatatagtc actgccgatt tgataggcat cagcaggtgt catgactcgt
    11221 ttttgatctc caactgcaac accagctgga cgaatccctg gtgtcagaca gataaaatct
    11281 ggattggtag cctgcttgat gacttgtact tcctgagccg agcaaacaac accatccaag
    11341 ccagcttcag ctgtcttctt ggcatagtga atcacagact cttgcagact ggtttggata
    11401 ttttgaaact cctgcatctg agcttctgac gttgatgtga gctgagttac agcgatcaat
    11461 ttggcttgac tcccaagacc ttcacgcgcc gccttcatca tctctacacc accagccgca
    11521 tggacattag tcatatcgac accaagctga gacaagacct tcatggctga cttgactgta
    11581 ttaggaatgt catgaagttt gagatccaaa aagacactat gacccaaacc ttttaagtag
    11641 gacacaatct caggccccgc tgcgtaataa agctccatcc ctaccttgag ataaaggctt
    11701 tcttctgctg ggaaaagagc taaaaattcc ttgaccgcct caaaactagg aaaatcaaga
    11761 gcaatgattg gacgatgttc tcgcataggt ttctcctttt acctttacta gtgagagagt
    11821 ctggccaaca gaagccacga aaaaaggaac ctgccaacag caaggttcca cgaaaaatgg
    11881 gtagtctcca ccctttcacc gtctaacctt agctgcctct ctggactgct ttaaaggttt
    11941 tctattattc tattatttct actagcactt gtcaagtaaa aacttagtca cgtatgtgtt
    12001 gactgctatt gaacacctca caagactctt tccaaaagaa tagttaaaga ctttcactta
    12061 ggacagagaa aaagagtttg cttgttaccc tttaattgaa caaatgggaa ttctgtttta
    12121 ttttgcggat gcctattcat cttggcaaag gggaagtaat gagaatgcga atggacttct
    12181 tcgtgagtat tttcttaaac cgatttggca acaattaccg aaaaaatcct taatggtgct
    12241 ttgctagctt attaatcacc gtccccaaaa atacttggat tacaagacag gttttgaagc
    12301 acttatagag gaaatttaaa aatgttgcat tttttattgc aatttatcag ttatcttatc
    12361 acccaaaact tctgcaatta gtatgagcac tattgttatt tttctttttt cgtacactca
    12421 aatcaaaata tagcaataag actattctgt agaattgctg tcaaaaagat cagttcattt
    12481 ttccttccca cttgtgttta taatcattaa aataactcaa ttaagaaatt ttctttttcc
    12541 taatagttat cgtaattaaa gttaatagaa gaacaatcca aataagtagt gttagattag
    12601 gaattagagt tttattccat gaaaatccct cccattttgt gaaaaactta gtaaacagtc
    12661 ccataaaact ataatctgtt atcttcgcaa ctgtcttatc caaaccagat agcatcggta
    12721 agaaagcaac taaaatcata gcaggaagac tgataacctg agctactact tggctcttcg
    12781 cggttaaacc tatcaataaa tagaataaaa tgatggataa agaggttaga agaacgattg
    12841 taatataatt aaaagtatgt acaattgtaa ctcctaaaat aagaggagta gttcccataa
    12901 tcacaaaagt tagcaaaaaa ggaagaaaca tcgttgataa aatgtattcg gatcctttaa
    12961 caccactcaa cagaagagtt tgtaaattgt acttttcttt ttcttcagac aagataatag
    13021 ttataggact tccaacagcc aaagccaaag aaaaaggtaa acacatcatc aaaagaacta
    13081 atgcctgttg atcgttgacc ttcccctgtg tttccataag atatttataa aaatatgtga
    13141 aagcaaaagg cactaaaact tgcaataaaa tactcttatt ggaaaggata atttgacaac
    13201 gcaaccatac caaagccaga aaccgtttaa gcatttagct ttcctcctgt taatgtgata
    13261 aaaatatctt ctaaagtagg ctcacatgaa tgaattgaaa aaatattttc cagatcagcc
    13321 tgagatacct gttctagtga tgtaaaatca aaagttatct gattcccatt ataatctgta
    13381 accttaatct ttttatcttt attatatctt tgaatgagtt cagaaggagc tccttgctca
    13441 actaatttcc ctttatttaa taaggcaaca taatcacata aaagagttgc ttcattcata
    13501 tcatgagtcg ttagaaaaat cgttgtccct gctgttttca attctaaaat taactcatga
    13561 attgttcgag aagttgtggg atctagacct gaggtcggtt catccagaaa gagtacagcg
    13621 ggtttgttga taagagctcg tgctaaaagc attcgttgcc tcattccagt ggataatttt
    13681 cctgctacca tcttgcgact atcatataat cctactcgct ttaacaaatt atcaacacgt
    13741 gatttactaa tattataaaa tttactataa aaaagaagat tgttatacag agacattttc
    13801 tcataaaatc cacttgtatc gctaaccaat ccaattctct ttaattcacc gcttgttaaa
    13861 ttttgagatt tttgtcccaa aataatagat tgtcctttat cggcaaggaa ctgcccagtc
    13921 agaatattaa tcgttgtggt ctttccagaa ccagatggtc ctaaaaatct aaccttcttt
    13981 aacagtgaag cttatattat ttaaagctat tttatcacca aaactttttg ataaatggct
    14041 aacttcaatc atactcattg cacctccttt atattctaca tttttactta acatctattt
    14101 acttatcttt atcaaataaa cgtcttaaaa aaaacaataa cattaagcat ataaccgtta
    14161 gcaagattgt ttcaatatta ttgataatac gatacattct caagcagtat aaaataatta
    14221 tatatatgca aatgctcatt atacctattt ttttaaacat actaagcctc cttgctaaat
    14281 aataaagtta tataataact aagacacact atacctaaca taataaatat ccactggtaa
    14341 ctgaataaat gttctaaaat taacaagcaa aaataaaatc tcaaacgatg aaaaaagcca
    14401 tactatattt tccaatgatg tttgaatatt tttattgtag taagcataat atatagcaaa
    14461 aataataccc aagaacaata ctattaattg tgtttctata acagtgaact gcccaatagc
    14521 agataaaaaa gctactcctc ctgataataa gcaaaaacaa actgaacgaa taaatgtttt
    14581 cgctctaata ttgttatctt tcactttatt atttttttac aaattggaca ttctccgttt
    14641 tccagaacat atttttcttg acttagagca tgatttctca tattataaac gtaagcgtta
    14701 tctattagga tttcattata acatcccaga aatattttaa ttattaaatt agtcattatc
    14761 cctcccaaaa tagcaggcat ctctggtgta gtcggccact ttgtcttatc agataaagtg
    14821 tacttattta tatctttgtt tatattccga caaccaaagc aagaaccgat ggtagggata
    14881 tatattatca gtaataagat agcatccatt aaaccctcct gatatgtagg atatcttatg
    14941 cgatacagca aattgattga caatttttat aatatcaatg ggcgtatcaa ttgctttaac
    15001 gataaaatct atactcccat attccgcaac aattttttct aattcttcta ctgaagaaac
    15061 tttaatagaa ataggtacta cctgaacatc tgaattatac ttgtgtattt tttcagaaag
    15121 aacattaatc ttctccttac caacatccga ctctgtataa agaatttgcc tatttaaatt
    15181 tgatggttct attatatcgt aatcagcgac aataagtttc ctcactccat acatacttag
    15241 acttagagat atataacatc ctaaggctcc tgctcctaat actaatactg ttttattctg
    15301 tattttatta tactcttcat tataaaataa cgaatagtaa cctttagttc tagaatatct
    15361 actatcaata tctatctcat ccgaaattaa aatttgttca tttaatagtt caaatgttag
    15421 tttactgcct aatacttctt ctatctctaa tagttcctta caactattaa atagatcgtt
    15481 gataaattct tgcgaaattt gaggttctat agctatttcc cgcatactcc gaccacgcaa
    15541 atacattata ccttcttttt tataagcaaa aacataacta tttacgtaga acatatttcg
    15601 tccttcctct catatataat atagaaagca gtatagaaac aaccacccct cctatactac
    15661 gaacaataat attaggtgtc aaaaactgta atagttgact caaagggaaa taaataataa
    15721 caaatgctat taaaagttga actatatatt gaaaattcaa gttattattg ttagaattca
    15781 agtatcttaa aataatacca ctatcatact gaaaaaaagg aattgaatta ataaaaatca
    15841 tacctaagtt aatgataaaa aataagagtc caagcaattt atacgatgtg aacaataaaa
    15901 caagtaaacc tgtaagtgat aaaatcgaat ttgaagcaat tcctgctaac ataattttca
    15961 aaaattgttt tttatttttt aagaattgca ccccacttaa atctacaaaa aaaagccaaa
    16021 ttaaacaaaa gtagcattat tccacaattt gggatctcaa tttgataatt attagcaa
//
LOCUS       DAVBCR010000045        16069 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCR010000045 DAVBCR010000000
VERSION     DAVBCR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.045"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(365..1138)
                     /locus_tag="VQ508_001351"
     CDS             complement(365..1138)
                     /locus_tag="VQ508_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2490337.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            1314..2717
                     /locus_tag="VQ508_001352"
     CDS             1314..2717
                     /locus_tag="VQ508_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2490338.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2832..3470
                     /locus_tag="VQ508_001353"
     CDS             2832..3470
                     /locus_tag="VQ508_001353"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2490339.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3482..3922
                     /locus_tag="VQ508_001354"
     CDS             3482..3922
                     /locus_tag="VQ508_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2490340.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3915..4346
                     /locus_tag="VQ508_001355"
     CDS             3915..4346
                     /locus_tag="VQ508_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2490341.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            4337..4807
                     /locus_tag="VQ508_001356"
     CDS             4337..4807
                     /locus_tag="VQ508_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2490342.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4832..5605
                     /locus_tag="VQ508_001357"
     CDS             4832..5605
                     /locus_tag="VQ508_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2490343.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5602..6402
                     /locus_tag="VQ508_001358"
     CDS             5602..6402
                     /locus_tag="VQ508_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2490344.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6461..8755
                     /locus_tag="VQ508_001359"
     CDS             6461..8755
                     /locus_tag="VQ508_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2490345.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8765..11881
                     /locus_tag="VQ508_001360"
     CDS             8765..11881
                     /locus_tag="VQ508_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2490346.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDSNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            11938..13704
                     /locus_tag="VQ508_001361"
     CDS             11938..13704
                     /locus_tag="VQ508_001361"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2490347.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            13850..15001
                     /locus_tag="VQ508_001362"
     CDS             13850..15001
                     /locus_tag="VQ508_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2490348.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            15146..16030
                     /locus_tag="VQ508_001363"
     CDS             15146..16030
                     /locus_tag="VQ508_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2490349.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         5288 a         2368 c         3433 g         4980 t
ORIGIN      
        1 tatatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc gacaaagtct
       61 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
      121 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
      181 aattgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
      241 aatactcttc gaaaatctct tcacatcatg tcagcttcgt cttgccgtgt ccatggggga
      301 cttgacttga tcagttttca aatctgtact ttgagcaagc tgagactagc ttcctatttg
      361 attttcattg aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt
      421 tttggtctgg caaggtgtca gaaataaagg ttgtgtattt ggagagggga ttaattttaa
      481 aaaatccagt cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt
      541 caataatatt cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat
      601 caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt
      661 ccttatcata gttgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga
      721 ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa
      781 aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca gtagtactag
      841 aatctagata gatacacata ccagaccgaa taaagtcttt agcgagacta gcgattagtc
      901 ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt gtgttcatag
      961 aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta
     1021 aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa
     1081 ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct tttatcataa
     1141 gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat
     1201 ttttatgaat attgggttaa catttgaaca ttattcaagt aagcgttcac atattgaaaa
     1261 aataaaacgt ggggattata ataaagttaa tcaaggacga agagagaaga aaaatggaag
     1321 cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg
     1381 aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag
     1441 ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg
     1501 ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac
     1561 tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat gaaagaacaa
     1621 agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag
     1681 gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc
     1741 ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct
     1801 tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta
     1861 ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca tctttacttc
     1921 ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg
     1981 atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta
     2041 agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta
     2101 cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag
     2161 atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag gaactaagac
     2221 gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga
     2281 aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg
     2341 atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag
     2401 atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag
     2461 agttattaga agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg
     2521 ctagagccag ttattttaac caaatgattg ctgatagaac tggtaaagag gttcttacag
     2581 gtttgactga ggctacagct gtggggaata ttgttgtgca gctcatagct atgggacaat
     2641 taaaagggat ggaagaggct caccatgtta ttgaggagtt tctacaatta gagagttatt
     2701 actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttag
     2761 cttttgtgca ggaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga
     2821 ggaggacaag gatgtcagat gtaaaacaag aattaattaa atatggtaag aagctagtag
     2881 aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat
     2941 tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag
     3001 tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata
     3061 tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa
     3121 cttatgcaac agtattagct tgtctcagag aaccacttcc agcgagtcat tatatgattg
     3181 cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggcacg aaagaattgg
     3241 ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa
     3301 ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt
     3361 gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg
     3421 agatggaatt gatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt
     3481 aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt taatggaaat
     3541 gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa
     3601 taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt
     3661 aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga
     3721 tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct
     3781 taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc
     3841 tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt
     3901 tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt gattttgcta
     3961 gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga
     4021 cattaggttt gatgcctgga gagaatattg tagagtttga gcattatttt aaaaatcaag
     4081 tggatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa
     4141 gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag
     4201 ggtttaatat tcctctccta gtggaattaa tatcaaatta tgattcaaaa atcaatttag
     4261 aagaaattgt tcacaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag
     4321 aggaggaaga agatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg
     4381 tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga
     4441 tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac cagtaggttt
     4501 aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa
     4561 aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa
     4621 tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt
     4681 aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa
     4741 gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt
     4801 tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac
     4861 attagttggg attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc
     4921 gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat
     4981 tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc
     5041 agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga
     5101 aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct
     5161 tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga
     5221 ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat
     5281 tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa
     5341 ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg
     5401 ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg
     5461 attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc
     5521 ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga
     5581 tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa
     5641 gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta
     5701 ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa agattcagct
     5761 tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt
     5821 attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt
     5881 actggtatta aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg
     5941 ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct
     6001 ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc
     6061 ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga
     6121 acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg
     6181 attccatcaa tggttggtat taattttgga ttagaattta agcaggggga acttgttatt
     6241 tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta
     6301 ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg
     6361 gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg
     6421 tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa
     6481 agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat
     6541 ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg
     6601 aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct
     6661 tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc
     6721 aagagatcaa agccactatg aattacttgg ggaactttac attgagcata tagatattca
     6781 gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt
     6841 tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta cgagttttaa
     6901 taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat
     6961 taatttgggt agaaataaac ggtttaatga cgaagtatct aaactggatt caagtacaat
     7021 tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc
     7081 taaggttacg gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac
     7141 agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc
     7201 tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa
     7261 atatcaggag caatttaata gagttgattt taaactagac tatagtaaag attgtcttag
     7321 cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt
     7381 gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgc
     7441 caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat
     7501 taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga
     7561 atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa
     7621 aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc
     7681 tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac
     7741 tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga
     7801 aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt
     7861 gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg
     7921 aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg
     7981 cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt
     8041 aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag aatggttaga
     8101 agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg ggctttatcc
     8161 ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta aaatcactat
     8221 caataggaga ttatcaaacg ctaacttttt atcttcacag gagagggagc aagcgattaa
     8281 taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct
     8341 gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt
     8401 gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg
     8461 taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt
     8521 atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt
     8581 aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt
     8641 ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt
     8701 acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata
     8761 agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg
     8821 gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac
     8881 gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa
     8941 cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa
     9001 accatatgca gcgattgaac ttcatcctgc aaaagtttgt aaaccaacaa gttgtattcc
     9061 acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct
     9121 aaacattcca gttttcttgg ttattatgtc ggctggggag cgtaatacag ttcctccaga
     9181 gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa
     9241 ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc
     9301 caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat
     9361 gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac
     9421 taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt
     9481 gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca
     9541 ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc
     9601 agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa
     9661 tttcgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa
     9721 aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt
     9781 tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg
     9841 attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata
     9901 tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc
     9961 taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa
    10021 ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag gtaattcctg
    10081 gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta
    10141 tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa
    10201 agaaaatcca aataatttac atattttatt gaataattac aggacagata agacagctat
    10261 gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg aagaaagaag aattagagtt
    10321 agcgaactgg ataagcaaaa attattccat caatcctgta gataatgact ttaggacaac
    10381 aacacttaca ttaaaagggc atactggtca taaacctcag ataaatataa gtggcgataa
    10441 aaatcattat acttatacag aaaattggga tgagaatacc catgtttata ccattacggt
    10501 taatcataat ggaatggtag agatgtctat aaatactgaa gggacaggcc cagtcccttt
    10561 cccaacacca gataaattta atgatggtaa tttgaatata gcatatgcaa aaccaacaac
    10621 acaaagttct gtagattaca atggagactc taatagagct gtggatggta acagaaatgg
    10681 taattttaac tctggttcgg taacacacac tagggcagat aatccctctt ggtgggaagt
    10741 cgatttgaaa aaaatggata aagttgggct tgttaaaatt tataatcgca cagatgctga
    10801 gactcaacgt ctatctaatt ttgatgtgat tctatatgac aataatagaa acgaagttgc
    10861 taagaaacat gttaataatt tgtcgggtga atctgttagt ctagatttca aagaaaaagg
    10921 agcaaggtat attaaagtta aattactaac gagtggagtg cctttgagtt tagcagaagt
    10981 agaggttttt agagaatcag atggtaagca atctgaagag gatatagata aaataacaga
    11041 agataaagta gtctctacaa ataaggtagc tactcaaagt tcaaccaatt atgagggtgt
    11101 agctgcttta gcagttgatg gtaataaaga tggagattac ggacatcatt cggtgactca
    11161 tactaaggca gattctaacg cttggtggca ggtcgatctg ggagaagagt ttacggtttc
    11221 taaagttgat atttataata gaacagatgc cgaacctcag cgtttatcta attttgatgt
    11281 tatttttcta tcttcatcag gagaagaagt ttttagaaga cattttgata aagtagttga
    11341 tggtttgtta tctttaaaag taccttctgt aggggctaag ctagtcaaaa tagaattaaa
    11401 atcagcagct attccgttaa gtttagcgga agttgaagtc tatggttcaa agagaactcc
    11461 gaagaaactt tctaatattg cattaaccaa agaaactcga cagagttcaa cggattacaa
    11521 tggtttttct cgtctagcag ttgatggaaa taaaaacgga gattatggtc atcattcagt
    11581 gactcatacc aaaggagatt ctccttcatg gtgggagata gatttagcac aaaccgaaga
    11641 attagaaaag ttaattattt ataatagaac agatgctgaa attcagagat tatcaaattt
    11701 tgatattatt atatatgatt caaataatca tgaagttttt aaacaacata ttgacagttt
    11761 agaaagcaat aatctatcca tagacttaaa agggctgaag ggaaaaaagg ttagaatttc
    11821 tttgagaaac gcaggaattc ctttaagttt ggcagaggta gaggtttata cttataagta
    11881 attttaaaaa ttatcatcgg tagtatgaaa gaaacagaaa aataagagga aaatagtatg
    11941 attcaacatc cacgtattgg tattcgtccg actattgatg gtcgtcgtca aggtgtacgc
    12001 gaatcacttg aagtacaaac aatgaacatg gctaaaagtg tggcagattt gatttcaagc
    12061 acattgaaat atccagatgg ggaacctgtg gaatgtgtga tttctccatc taccattggt
    12121 cgtgttccag aggctgcagc ttcccatgag ttgtttaaaa aatcaaatgt ttgcgcaaca
    12181 attacagtta caccatgctg gtgttatggt agtgaaacta tggatatgtc tccagatatt
    12241 cctcatgcta tttggggatt taatgggaca gaacgcccag gagctgtcta tcttgcagct
    12301 gtactagctt cacatgctca aaaagggatt ccagcctttg ggatttatgg tagagatgtt
    12361 caggaagcta atgatacaga tattccagaa gatgtcaaag aaaaactttt acgttatgcg
    12421 cgtgcagctc ttgcaactgg cttgatgaga gacactgctt acctatcaat gggtagtgtt
    12481 tcgatgggga ttggtggttc tattgtaaat ccggatttct tccaagaata cttaggaatg
    12541 cgaaatgaat cggtagatat gacggagttc acacgccgta tggaccgtgg tatttacgac
    12601 cctgaagagt ttgaacgtgc tatggtttgg gtaaaagaac atatcaaaga aggagttgac
    12661 cgaaatcgtg aggatttgat tctttctaaa gaagagaaag aaaaacaatg ggaatttgtt
    12721 attaagatgt tcatgattgg acgtgactta atggttggta acccaagact tgctgaactt
    12781 ggttttgagg aagaagcagt tggtcaccat gctttagtag ctggtttcca aggtcaacgt
    12841 cagtggacag accattttcc aaatggagat tttatggaaa ctttcctcaa tactcagttt
    12901 gactggaatg gtattcgaaa accatttgta tttgcgacag agaatgattc actaaatggt
    12961 gtgtctatgc tctttaatta tctattaaca aatactccac aaatctttgc tgatgtgcgt
    13021 acttattgga gtccagaggc tgttgaacgt gtaacaggac atactttaga gggtcgtgct
    13081 gcagctggct tcttacatct aatcaactct ggatcttgta cattggatgg tacaggtcaa
    13141 gctactcgag atggcaaacc tgttatgaaa ccattctggg agttggaaga aagtgaagtg
    13201 caggctatgc ttgaaaatac agacttccca ccagcaaacc gcgaatactt ccgtggagga
    13261 ggattctcaa ctcgtttctt gacgaagggg gatatgccag taacaatggt acgtctcaat
    13321 cttctaaaag gggttggtcc agtgctacaa attgcagaag gttacacact tgaacttcct
    13381 gaagatgttc accatacttt agataatcgt acagatccag gatggccaac tacttggttt
    13441 gctccacgtt tgacaggaaa aggtgctttc aagtctgtct atgacgtcat gaataattgg
    13501 ggagctaatc acggagccat aacatatggc cacattggag cagacttgat taccttggct
    13561 tctatgttga gaattcctgt caatatgcat aatgtacctg aggaagatat ctttagacct
    13621 aaaaattggt ccttatttgg aacagaagat ctagaatcag cagactatcg tgcatgtcag
    13681 ttgttgggac cactacataa ataaaacttg tttatatagg aggtgaactt acgtccctcc
    13741 tatcctttta aaaagatttg ttaaacaatt cacaaataat tgaaaacgaa tacaaaaagt
    13801 aatataatga tgttaaatag atagcgcaaa ggcgcaggag gaaaattata tggctacatt
    13861 ttatgttccg gcagtcaacc ttattggtaa aggtgttgta aatgaagtgg gtccttatat
    13921 caaggaactt ggctataaaa aggcactttt ggttacagat aagtacatcg aaggcagtga
    13981 cattttacct aaggttttaa aaccactgga tacagaagga atcgaatatg tcatctttag
    14041 cgatgtagag ccaaacccta cttgtaagaa tgtcacagat ggggtagctg ctttgcaaga
    14101 acatggatgt gattttatca tcagtcttgg tgggggttct ccacaggatg cagctagttg
    14161 tatttctatc atggctacaa atggtggaag accacaggat tatgaagggc ttcataagtc
    14221 tgctaaaaaa ggcttgccag ttgtggctat caatacaacg gcaggtacat cagcagaaat
    14281 taccattaac tatgtgatta ctgatgaaga acgcaaggtt aagatggtaa tggttgacaa
    14341 gaatagcctt gccttgattt ctgtcaatga ccctgaactc atgctttcca aacctaaagg
    14401 cctgactgct gctactggta tggatgctct gactcatgct gttgaagctt tggtaacacc
    14461 tggtgcttat gatgtaacca agaaactgtc tattggtgct attgagctta tcaaggaata
    14521 tcttcctcgt gctgtagaaa atggacatga tattgaagcg cgtgaaggta tggtcaatgc
    14581 catcttcctt ggtggtatga gctttaataa tgctggtctt ggctatgttc actcaatggc
    14641 tcaccaactc ggtgcagtat ataatttgcc acatggcgtg tgctgtgcca tgttgctacc
    14701 agttatagaa cgtgaaaatg ctaaacgtgt accagaagct ttccgcaatg ttgccaaagc
    14761 cttgggactt catgtagaag gtaaatcaga tcaagaatgt gccgattatg cgattgctga
    14821 gattgagaag ctttctgaga cagtaggtat tcctaagaaa ctgactgaac ttggtattga
    14881 agaaaaagat ttcgactttg aatacctttc taagaatgcc ttgattgatg cctgcgcacc
    14941 aggaaatcca tttatgccaa ccttagaaga aacgattgcc ttttataaag agttatttta
    15001 gcaagagatt ggacgacaat aggacaattc acctctaatt attgacattt caggaaaaat
    15061 cgcagtaaaa acggcacagc cttctttaaa tgtgctataa tactagaaaa atacttgtgg
    15121 aggttccatt atggcaatat ttttcatgat ttttctgatt gtttgtgtgc tcctattggt
    15181 gatagtcaca ctgagtacag tttatgtggt tcgtcagcag tcggtggcga ttattgaacg
    15241 ctttgggaaa taccaaaagg ttgccaatag cggtattcat attcgcttgc cttttgggat
    15301 tgactcgatt gcagcacgga ttcagttgcg cttgttgcaa agtgatattg tggttgagac
    15361 taagaccaag gacaatgtgt tcgttatgat gaatgtagcg actcagtacc gtgtcaacga
    15421 gcagagcgtg acagatgctt actataaact catacgtcca gaatctcaga ttaaatctta
    15481 tatcgaagat gctcttcgtt cttctgttcc aaaattaacc ttggatgaat tgtttgagaa
    15541 aaaagatgag attgcccttg aagttcaaca ccaagtagca gaagaaatga ccacttacgg
    15601 ctacattatc gtgaaaacct tgattaccaa ggtcgaacca gatgcagaag ttaagcaatc
    15661 tatgaatgaa atcaatgcgg cgcaacgtaa gcgggtcgca gcacaagaat tggcggaagc
    15721 tgacaagatt aaaattgtca ctgcagctga agccgaagca gaaaaagacc gccttcatgg
    15781 tgtggggatt gcccaacaac gtaaggcgat tgtggatgga ttggcagagt ctatcaccga
    15841 actcaaggaa gccaatgttg gaatgacaga agaacaaatc atgtctatcc tcttgaccaa
    15901 ccagtatttg gataccttga atacctttgc ctctaaagga aatcaaacca tctttttacc
    15961 aaatactcca aatggtgtgg atgatatccg aacacaaatc ttgtcagccc ttcgcgctga
    16021 gaagaaataa tagactaata ctcttcgaaa atctcttcaa accacgtca
//
LOCUS       DAVBCR010000046        15641 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCR010000046 DAVBCR010000000
VERSION     DAVBCR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.046"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(48..797)
                     /locus_tag="VQ508_001364"
     CDS             complement(48..797)
                     /locus_tag="VQ508_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2490350.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            930..3266
                     /locus_tag="VQ508_001365"
     CDS             930..3266
                     /locus_tag="VQ508_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2490351.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3368..4045
                     /locus_tag="VQ508_001366"
     CDS             3368..4045
                     /locus_tag="VQ508_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2490352.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4032..4829
                     /locus_tag="VQ508_001367"
     CDS             4032..4829
                     /locus_tag="VQ508_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2490353.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4846..5949
                     /locus_tag="VQ508_001368"
     CDS             4846..5949
                     /locus_tag="VQ508_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2490354.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            5956..6147
                     /locus_tag="VQ508_001369"
     CDS             5956..6147
                     /locus_tag="VQ508_001369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490355.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            6250..7269
                     /gene="galE"
                     /locus_tag="VQ508_001370"
     CDS             6250..7269
                     /gene="galE"
                     /locus_tag="VQ508_001370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2490356.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7271..8224
                     /locus_tag="VQ508_001371"
     CDS             7271..8224
                     /locus_tag="VQ508_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2490357.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            8376..9041
                     /locus_tag="VQ508_001372"
     CDS             8376..9041
                     /locus_tag="VQ508_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490358.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            9080..9181
                     /locus_tag="VQ508_001373"
                     /pseudo
     CDS             9080..9181
                     /locus_tag="VQ508_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(9232..9438)
                     /locus_tag="VQ508_001374"
     CDS             complement(9232..9438)
                     /locus_tag="VQ508_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2490359.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            9490..9966
                     /locus_tag="VQ508_001375"
     CDS             9490..9966
                     /locus_tag="VQ508_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2490360.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9975..10646
                     /locus_tag="VQ508_001376"
     CDS             9975..10646
                     /locus_tag="VQ508_001376"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2490361.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            10777..11115
                     /locus_tag="VQ508_001377"
     CDS             10777..11115
                     /locus_tag="VQ508_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2490362.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            11118..11747
                     /locus_tag="VQ508_001378"
     CDS             11118..11747
                     /locus_tag="VQ508_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2490363.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11987..13138
                     /locus_tag="VQ508_001379"
     CDS             11987..13138
                     /locus_tag="VQ508_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490364.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            13451..14200
                     /gene="truA"
                     /locus_tag="VQ508_001380"
     CDS             13451..14200
                     /gene="truA"
                     /locus_tag="VQ508_001380"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2490365.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            14190..14963
                     /locus_tag="VQ508_001381"
     CDS             14190..14963
                     /locus_tag="VQ508_001381"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2490366.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14941..15402
                     /locus_tag="VQ508_001382"
     CDS             14941..15402
                     /locus_tag="VQ508_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2490367.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4473 a         2906 c         3613 g         4649 t
ORIGIN      
        1 tgatatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
       61 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
      121 atagcgagaa taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
      181 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
      241 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
      301 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaagtcatg
      361 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
      421 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
      481 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
      541 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
      601 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
      661 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
      721 gtattatgat agtgagcatt gccattgatg gaccataaga gcaatacaac taatccacgc
      781 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
      841 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
      901 ctgttaaaaa aaatgatata atagaattta tggataaaaa taagattatg ggattaaccc
      961 aaagagaaat caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
     1021 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
     1081 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
     1141 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
     1201 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
     1261 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
     1321 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
     1381 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
     1441 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
     1501 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
     1561 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
     1621 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
     1681 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
     1741 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
     1801 gcgtggatat gctctgtctg gacaagacgg gtaccatcac ccaaggaaag atgcaggtgg
     1861 aggctgttct tccactgacg gaaacttatg gtgaagaggc tgttgccagc atcctgacca
     1921 gctatatggc ccatagtgag gataaaaatc caacagctca agccattcgc cagagattcc
     1981 aaggtcaagt agtctatcct atgctctcta atcttccctt ctccagcgac cgcaagtggg
     2041 gagccatgga gttagaaggt ttgggaatag ttttcttagg ggcacctgag atgttgctgg
     2101 attctgaggt cccagaagcc agagaggcct tggagagagg atcacgtgtc ttggtcttag
     2161 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
     2221 tagccttgct ggaaatcttg gatcctattc gagagggagc ggcagagacg ctgaactatc
     2281 tccgttctca ggaagtgggg ctcaagatta tctctggtga caatccagtt acggtatcca
     2341 gcattgccca gaaggctggt tttgcggact atcacagcta tgtcgattgt tcaaaaatca
     2401 cggatgagga attgattgct ctggcggagg agacagcgat tttcggacgt gtttcccctc
     2461 atcaaaagaa actcattatc caaacgctga aaaaagcggg tcatacaaca gctatgactg
     2521 gggatggagt taatgatatc ctggctcttc gtgaggcgga ttgttctatc gtaatggcgg
     2581 agggagaccc ggcaacccgt cagattgcca acctggttct cttgaactca gactttaatg
     2641 atgttcctga gattcttttt gaaggtcgtc gtgtggtcaa taacattgcc cacatcgctc
     2701 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
     2761 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
     2821 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
     2881 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agtgccctca
     2941 tggtcgtgtt tagcgttctt tttgtgaaaa tctttggaac gagtcaaggt tggtctgagt
     3001 tagaaatctc aactctcctc tattatctct tggggtcaat tggtttctta tccgtattta
     3061 gagcctgcat gccattcact ctatggcgag tcctcttgat tgtctggtca gtaggaggct
     3121 tcctagccac agctctcttc ccaagaatcc aaaaactgct tgaaatttca accttgacag
     3181 aacaaacgtt gcctgtttat ggtgtcatga tgttggtctt tacagtgatt ttcatcctga
     3241 ctagtcgcta tcaagctaga aaataaagaa agactgcaat ctgtggattg cagttttttt
     3301 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt ttggaaagtg
     3361 agagaagatg atttcaaaga gattagaatt ggtagcttcc tttgtgtcac agggggctat
     3421 tttactagat gtgggaagtg accatgctta tctgcctatc gagttggttg agagaggcca
     3481 aatcaaaagc gctattgcag gtgaggtggt ggaaggtccc tatcagtctg cggttaaaaa
     3541 tgttgaggct cacggcctaa aggagaaaat ccaagtccgt ttagccaatg gcttggcagc
     3601 ttttgaagag actgaccaag tgtctgtcat taccattgct ggcatgggtg gtcgtttgat
     3661 tgctaggatt ttagaagaag gtttggggaa gttagctaat gtagagcgtt tgatcctcca
     3721 gcccaataat cgtgaagacg acttgcgtat ctggctacag gatcatggat tccagattgt
     3781 agcagaaagc atcttagaag aagctggaaa gttttatgag attttggtgg tggaagcagg
     3841 acaaatgaag ctatcagcca gtgatgttcg ctttggtccc ttcttgtcca aagaagtcag
     3901 tccagtattt gtccaaaaat ggcaaaaaga agctgagaag ctagagttcg ctctcggaca
     3961 aatcccagaa aaaaatctgg aagaacgtca agttctagta gataagattc aagctatcaa
     4021 ggaggtgctc catgttagca agtgaagtga ttcatgctta tgaagccttt tgccctcagg
     4081 aattttctat ggagggagac agccgtggtc tgcaaattgg cactttagac aagggtatcc
     4141 aaagggtcat ggttgccctc gatattcgtg aagagacagt ggcagaggcc attgaaaagg
     4201 gtgtggactt gattatcgtc aagcacgcgc ctatctttcg tcctatcaag gacttgcttg
     4261 ccagccgtcc gcaaaatcag atttacatcg acctgattaa gcatgacatc gcagtttatg
     4321 tcagccatac caatattgat atcgttgaaa atgggctcaa tgactggttt tgtcagatgc
     4381 taggaatcga ggagacgact tatctgcagg aaacaggtcc agaacgtgga attggacgta
     4441 ttgggaatat tcagcctcag acattttggg aattggccca acaggtcaag caagtctttg
     4501 acctagatag ccttcgaatg gtgcattatc aagagaatga tttgcagaag cctatttcaa
     4561 gagtagcaat ttgtggtgga agcgggcagt ctttctataa ggatgctttg gcaaaggggg
     4621 cagatgtcta tatcactggt gatatctact accatactgc tcaggatatg ctgtctgatg
     4681 gcttgttagc attggatcca ggtcactata tcgaagtgct ttttgtggaa aaaatcgcag
     4741 cactccttac tcaatggaaa gagaaaaaag gctgggaact agagatttta cctagtcaag
     4801 catcgaccaa tcctttccgt catatctagt tagaaagtga agacaatgaa aaaagttgcc
     4861 attattggag caggaattgt gggagcaaca gctgcctact acctctcgag agaaagtgac
     4921 ctagaggtga ccgtttttga ccatgagcaa ggtcaagcca ccaaggccgc agcaggaatt
     4981 atcagtcctt ggttttccaa acgccgtaat aaagcctggt acaagatggc gcgcttgggg
     5041 gctgattttt atgtggattt attagctgat ttagagaaat caggacaaaa aatcgacttt
     5101 taccagcgtt cgggagtctt tctcttgaaa aaggatgaat ccaagttgga agaactttat
     5161 caactggccc tccagcgcag agaagaatct cccttgatag ggcaattagc cattctggac
     5221 caagcctcag ctaatgaatt attccctggt ttgcagggat ttgaccgcct gctctatgct
     5281 tctggtggag cgagagtaga tggccaactc ttggtaactc gtctgctaga agccagtcat
     5341 gtcaagctgg tcaaagaaaa agtgactctg acaccgttag catcaggcta ccagattggt
     5401 gaagaggagt ttgagcaggt tattttggcg acgggagctt ggttggggga catgttagaa
     5461 cccttgggtt atgaagtaga tgttcgtcct caaaaaggac aactacgaga ttatcagctg
     5521 gtccaagaca tggaagctta ccctgttgtc atgccagaag gggagtggga tttgattcct
     5581 tttgcaggtg ggaaattgtc cttaggcgct acccacgaaa atgacatggg atttgatttg
     5641 acggtagatg aaaccttgct ccaacaaatg gaggaggcca ccttgactca ctatctgatt
     5701 ttggctgaag ctacttcaaa atctgagcgt gttggaatcc gtgcctacac cagtgatttc
     5761 tctcctttct ttgggcaggt gcctgactta actggtgtct atgcagccag tggactaggt
     5821 tcatcaggcc tcacaactgg tcctattatt ggttaccatt tagctcaact gatccaagac
     5881 aaggagttga ccttggaccc tgaaaactac ccaattgaaa actatgtcaa acgagtaaaa
     5941 agcgaataat actcaatgaa aatcaaagaa caaactagga aactagccgc gggttactca
     6001 aagcactgct ttgaggttgt ggatagaact gacgaagtca gctcaaaaca ctgttttgag
     6061 gttgtagata gaactgacga agtcagtaac catacccatg gcaaggcgac gttgacgtgg
     6121 tttgaattgg attttcgaag agtataagca ttttactgaa attttatcag gtagtttagg
     6181 atggcaaatg acattccctg tcaaaaatgg taaaataaga aaaaataatc tgagaatcga
     6241 ggaaaaaaga tgcaagaaaa gattttggta actggtggtg ccggttttat tggaacccac
     6301 actgttattg aattgatcca agcaggccat caagttgttg tggtggataa tcttgtcaat
     6361 agcaatcgta agagtttaga agttgttgaa agaatcacag gagttgagat tcctttctat
     6421 gaggcagata ttcgtgacac agctaccctc agagatattt tcaagcagga agaaccgact
     6481 ggtgtcattc actttgctgg tttgaaggct gttggcgaat caacacgtat ccctcttgcc
     6541 tactatgaca acaatatcgc tggaactgtc agccttttga aagccataga agaaaacaac
     6601 tgtaaaaaca tcatcttcag ttcttctgcg acagtttacg gggatccgca cacagtgccc
     6661 atcttggaag atttcccact ttcagtgacc aacccatacg gtcgtactaa gctcatgcta
     6721 gaggaaattt tgactgatat ttacaaagca gactcagaat ggaatgttgt cttgcttcgt
     6781 tactttaacc caatcggagc ccatgagagt ggtgatttgg gagaaaatcc aaacggtatt
     6841 ccaaacaatc tcttgccata tgtgactcaa gtagccgttg gaaaattaga gcaagtgcaa
     6901 gtgtttggag acgattacga tacggaagat ggaacaggtg ttcgtgacta tatccacgtt
     6961 gtcgatttgg ctaagggtca cgttgcagct ttgaaaaaaa tccaaaaagg ttcaggacta
     7021 aacgtttata accttggaac tggtaaaggt tactcagttc ttgaaattat ccaaaacatg
     7081 gaaaaagcgg tgggacgtcc tattccttac cgtatcgtag aacgtcgccc aggtgatatc
     7141 gctgcctgct actcagaccc agcaaaagct aaagcagaac tcggttggga agcagaactc
     7201 gacatcaccc aaatgtgtga agacgcatgg cgttggcaaa gcaagcatcc aaatggattt
     7261 gaagactaag atgatgattt caatcatcgt cccttgttta aacgaagagg aagtacttcc
     7321 tcttttttat caggctctgg aagctttact tccagatttg gaaacagaaa tcgagtatgt
     7381 ctttgtcgat gatggatcaa gtgatgggac cttggaactc ttaaaggcct atcgggagca
     7441 aaatccggca gtccattata tttctttctc tcgaaatttt ggcaaagaag cagcccttta
     7501 tgcaggcttg caatatgcga caggagattt ggtggtggtg atggatgcag acctccaaga
     7561 tcctcctagt atgttgtttg agatgaaaaa tgtactagac aaaaatgtag acttggactg
     7621 cgttgggaca cggagaacta gtcgggaggg agaacccttc tttcgcagtt tctgtgctgt
     7681 tctcttttat cgcctcatgc aaaaaattag cccagtggct ctgccgtcgg gtgtccgtga
     7741 ttttcgtatg atgagaaggt ctgtagtcga tgccatttta agcttgaccg agtccaatcg
     7801 tttttcaaag gggctcttcg cctgggtggg tttcaaaacc tactatttgg actatccaaa
     7861 tgtcgaaagg caggctggca agaccagttg gagttttagg aaactctttt tctactccat
     7921 tgaagggatt attaattttt cagattttcc cttgattata gcctttgtag ctggtctcct
     7981 atcttgtttt ctttctctgc tgatgacctt ttttgttgtg gttcggaccc tcattttggg
     8041 caatccgaca tctggttgga cctctctgat ggctgttatt ctctttcttg gaggcattca
     8101 actcttgacc attgggattc tcggcaagta tatcagtaag atttatttag agactaaaaa
     8161 aagaccactt tatcttatca aagaaaaaag tgatgatagg ctaaaaaaga tagaatttat
     8221 ctgacatagt cactttttgt tatttgtaat tcttttagtt cggagtttgg gtgattttta
     8281 aaagaattag ggatttgaaa tgatttaaaa aatctatgta gactaaattt aaattgtagt
     8341 gacttaataa ataaaaaatt taggagtatt atgttatgct aagtaaagag gattatactg
     8401 aagaaatcgg gctaataaag aaacaaaatt atgtagaggc tgaattgtat cctatagttg
     8461 ctgatataat taagccaact ttaaaagata gtttatcaaa aagatatgtc ttcggaagac
     8521 aaagaagggg tttgggacaa atttattatg gtctaagtaa ttttcctgat atagttattt
     8581 tagataagac ttatgaaaac aaatctagga aaagtataaa aattgaagaa tggaagaaac
     8641 ttcgaggatg cgtggaagtt aagaatttaa attatagttt aattacagag gaaaaaatca
     8701 aatcaaccat atcgaacagt tttgaacata taactggaga gatggaacaa ctaactggag
     8761 agatgggaca acttattgga gaccttttat ggtataaaaa agtaatttat acaaatggaa
     8821 ttgaatggag atttctaagt ttagatgaca aggaagagat agataataca attgttgaag
     8881 tagttaacaa aagaatagaa acagaagaag cgggaaattc ttttgattgg tggaagaata
     8941 ttaaagattt atcgttcaat tatacggata tatgcctatc taaagattgt agacaagagt
     9001 ggaatgaatt tgtaaaaagg gtaaaggaaa tagaatggta gaagtctatt ctcattgaga
     9061 aatttagatt ttaagttaca gtaagattta tttagagact aaaaaaagac cactttatct
     9121 tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa aataaagtga aaagactata
     9181 attttacatg gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc
     9241 ctggtttcca atcagggcgc gagttgggca atttttaaca gcctctaaga catcctgact
     9301 aggagaaatt tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc
     9361 gtggtaatca aataaatcag aataagtttg gcaaagccca caggcgatac atcgttcagg
     9421 tataagtgtg attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag
     9481 gagtaaggta tggcaaaaga accgtggcaa gaagatatct atgatcaaga agaatcaaga
     9541 gcagagcgtc ggcatcgaaa ccacggaggg gctgatagga tggctaatcg tattttgacg
     9601 atcctagcta gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta
     9661 tcatcggggg gaagtaatcg cacagcagcc ttaaaagact ttcatgattc tgatgcaagt
     9721 gtagtacaaa tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct
     9781 acttctagta gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga
     9841 gaaggggaag cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg
     9901 aatcctgggc acatggctac aggatcttgg tttgctaatc caggtgatgt tataaaaata
     9961 aaataggagc cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta
    10021 agagtacggt cgcaaagatt attgctaagg attttggatt cacctacctt gatacaggag
    10081 ctatgtatcg tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg
    10141 aagcccttct agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag
    10201 accagcttgt ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga
    10261 ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc
    10321 aacaagaaat tgcccagcaa ggcgggattg tcatggatgg tcgcgatatt ggaactgttg
    10381 tattgccaca agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc
    10441 gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga ctttgaaacc ctaaaaaagg
    10501 aaattgctgc gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag
    10561 aggatgctgt ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca
    10621 aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt
    10681 ttctaaattt gtcaaaatgc tgattttagg gtataatagt tgctgttcga gaaattttga
    10741 ttttcaaaga atagtgaatg atgataagga gaaaccatga acaatttacc aaattgtcca
    10801 aaatgtaact cagagtatgt ctacgaagac ggtgccctac tggtttgccc agagtgtgct
    10861 catgagtgga atcctgctga agttgcagaa gtagaagaag gtcttgtcgc tatcgatgcc
    10921 aacggaaata aattggctga tggtgataca gtaactctca tcaaggactt gaaagtaaaa
    10981 ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa atatccgcat cgtagaaggc
    11041 gaccacaata tcgactgtaa gattgatggt tttggtgcca tgaaacttaa atcagagttt
    11101 gtgaggaaga tttaagcatg tcaaaacact ataaacttgt attttatagc cgtatcttct
    11161 tgtttctagc ggcttttacg ggagtttatc ttgaaattac taagcatggt ggttttggga
    11221 tgcttctcta ttacacggtt ctgtccaacc tcttggtaac tatttttacc ctttatcttc
    11281 taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc aagtctcttg cgcttaaaag
    11341 gtggggtcac catgagtatc atgattacct gtgtgattta ccatttcctc ttggcgccca
    11401 ttgcgactaa tttctatacc ctagaaaatt tcctttgcca ctatatcgtt cccatctggt
    11461 ttttagcgga taccctcttt tttgacaaac agggtcaata caagatttgg gatccagcag
    11521 tgtggaccat tttacccttt ctgtatatga tgtttgctct ttttaatggc ttggttctaa
    11581 aactcaatat tccaaatgcc aaggataatc ctttccctta cttctttttg aatgtgaaca
    11641 agggttggaa tgttgtgttt aagtggtgtc tgattatctt tgttgcctat atggtagcag
    11701 gatttatttt ctactttatc aagcaaatca agagaaagtc atcctaatac tcttcgaaaa
    11761 tctcttcaaa ccacgtcagt ttccatctgc aacctcaaaa cactgttttg aacaacctgc
    11821 ggctagcttc ctagtttgct ctttgatttt cattgagtat aagatacaag ggattcattt
    11881 tcgaatttaa gaaggagtct acaaaccaat gaggcttctt cttttcttgc ttatttgata
    11941 actatcaaaa aagtagaaga ttaagagaaa tatagaaaga gatttcatga aacaattttt
    12001 agaacgggcc agcattttgg ctctctccct cgttttgatt acctcctttt ccatttcgag
    12061 tgccctacca gccatgtttg actattatca gggttattct aaggaacaaa ttgagctctt
    12121 ggtgagcttg ccttcctttg gaatcatgat gatgttactg ctaaatggtt tcttagaaaa
    12181 aatatttcct gagcgcttac agattagttt gggcttgctg attttatcat tgagcggtac
    12241 agctcccttc tggtaccaag cctatccctt tgtctttgga acacggcttc tctttggttt
    12301 gggtcttggg atgatcaatg ccaaggccat ttctattatc agtgaacgct accaaggaaa
    12361 aaggcgaatt cagatgttag ggctacgcgc ttctgcagag gtcgttggag cttctctcat
    12421 taccttggcc gtcggtcagt tgttggcctt tggttggaca gctatctttc tagcctatag
    12481 tgctggattt ttggtgctgc ccctttatct gctctttgtc ccttatggaa aatcaaagaa
    12541 agaagtcaag aaaagagcga aggaagcaag tcgtttaact cgagaaatga aaggcttgat
    12601 ttttacctta gctatcgaag cggcagttgt agtttgtacc aatacagcta ttaccatccg
    12661 tattccaagt ttgatggtgg aaagaggatt gggggatgcc cagttatcta gttttgttct
    12721 tagtatcatg cagttgatcg ggattgtggc tggggtgagt ttttctttct tgatttctat
    12781 ctttaaagag aaactgctcc tctggtctgg tattaccttt ggcttggggc aaatcgtgat
    12841 tgccttgtct tcatccttgt gggtggtagt agcaggaagt gttctggctg gatttgccta
    12901 tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa cgaattccag ctaaactcct
    12961 caatcaagca acttcatttg ctgtattagg ctgtagtttc ggagccttta cgaccccatt
    13021 tgttctaggt gcaattggct tactaactca caatgggatg ttggtcttta gtatcttagg
    13081 aggttggttg attgtaatct ctatctttgt catgtaccta cttcagaaga gagcttagga
    13141 ttgattccta agtttttctt ttgatactca atgaaaatca aagagcatag ttgattgagt
    13201 ttggaatagt acgctgtagc tgctaaaata tttctagaaa ttaatttgac ttttctaata
    13261 gagttattca tatcttattt caatctacta tagaaagcta gccgcaggtt gctcaaaata
    13321 gtgttttgag gttgcagatg gaaactgacg tggtttgaag agattttcga agagtatgag
    13381 atttttatac ccatacaata tttgttttag gtccttgttt acagactaat ttcttgataa
    13441 aatagaggtt atgacgagat ataaagcaac tatttcctat gatggttatg cctttgctgg
    13501 ctttcagcgc cagcctcatg cgcgtagcgt tcaggaagaa attgaaaaaa ccttgaccag
    13561 attaaataaa gggcaagcca ttactgttca cggtgctggt aggacagata gtggggttca
    13621 tgccctggga caggtcattc attttgacct gccttatcag atggatgagg agaaactccg
    13681 ttttgccttg gatacccagt ctcctgaaga tattgatgtg atttcgattg agcttgtggc
    13741 agatgatttt cattgccgtt atgccaagca tagcaagacc tatgagttta ctgtggatag
    13801 aggacgtccc aaaaatccga tgcgccgtca ctatgccacc cactttccct acccactcga
    13861 tgtggaacga atgcagattg caatcaaaaa gctagaggga acccatgatt ttaccggttt
    13921 tacagcctct gggactagtg tagaggataa ggttcgcacc atcacagaag ctagtttaat
    13981 agtcgatgag acaggacaat ttttgacctt taccttttca ggaaatggtt tcttgtataa
    14041 acagattcgc aatatggtgg ggactcttct caaaatcggt aacaaccgca tgccagtaga
    14101 gcagattgat ctcatcttgg agaagaagga caggcaactt gcaggtccca ctgcagcacc
    14161 aaatggtttg tatttaaagg agattcgtta tgaagaataa tcgtatttta gcactttctg
    14221 gaaatgatat ttttagtggt ggtggactgt cagctgattt ggctacctat accttgaacg
    14281 gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt gacagaaaaa ggatttgaag
    14341 tctttccaac tgatgatacc atttttcaac atgaattaga tagcttgcgt gatgtggaat
    14401 ttgggggaat taagattggt cttctcccta ctgtcagtgt ggctgagaag gccttggact
    14461 ttatcaaaca acgcccagga gtacctgtgg tgttggatcc tgtcttggtc tgcaaggaaa
    14521 cgcatgatgt agctgtcagt gagctctgcc aagagttgat tcgctttttc ccttatgtca
    14581 gtgtgattac gcctaatctc ccagaagcag aattattatc cggtcaggaa attaaaacct
    14641 tggaagacat gaaaactgca gcgcagaaat tgcatgattt aggagcgcca gcagtcatta
    14701 tcaagggagg caatcgtctt agtcaggaca aggctgtgga tgtcttttat gatggacaga
    14761 cctttactat cctagaaaat ccagttatcc aaggccaaaa tgctggtgca ggttgtacct
    14821 ttgcctctag cattgccagt cacttgatta aaggtgataa acttttgcca gcagtagaaa
    14881 gctctaaggc tttcgtttat cgtgctattg cacaagcaga tcagtatgga gtaagacaat
    14941 atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc tattaaccgc cctttctgtg
    15001 gttctaggtt atttcttaaa aatcccaaca cctacaggaa ttctaactct tttagatgct
    15061 ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag gagcggtagt cggaggacta
    15121 gcaagtttct tgattgacct cttatcaggc taccctcagt ggatgttctt tagcttggtc
    15181 aaccatggct tgcagggatt tttcgcagga tttaaaggaa aaagtcagtg gttaggcctt
    15241 attttagcaa ctattgccat ggtaggaggc tacgccttgg gttctacttt gatgaatggc
    15301 tgggcagcag ccctcccaga aattctacca aatttcatgc aaaatatggt agggatgatt
    15361 gtaggattta ttcttagtca aagtatcaag aagattaagt aatactcttc gaaaatctct
    15421 tcaaaccacg tcagcttcca tctgcaacct caaaacactg ttttgagcag cctgaggcta
    15481 gctttctagt ttgcactttg attttcattg agtataaaga ggttgagtaa aaagtatttc
    15541 aaataagaaa aacgcatagt atcaggtgtt gaatgaactg ccccccaaaa agttagacag
    15601 aaaaaatcta actttttggg gtgtttttat tatgaaatta a
//
LOCUS       DAVBCR010000047        15592 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCR010000047 DAVBCR010000000
VERSION     DAVBCR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.047"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..741)
                     /locus_tag="VQ508_001383"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="VQ508_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ508_001384"
     CDS             complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ508_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2490368.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ508_001385"
     CDS             complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ508_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2490369.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3071..3466)
                     /locus_tag="VQ508_001386"
     CDS             complement(3071..3466)
                     /locus_tag="VQ508_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2490370.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3544..4305)
                     /locus_tag="VQ508_001387"
     CDS             complement(3544..4305)
                     /locus_tag="VQ508_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2490371.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4338..4964)
                     /locus_tag="VQ508_001388"
     CDS             complement(4338..4964)
                     /locus_tag="VQ508_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2490372.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4980..5297)
                     /locus_tag="VQ508_001389"
     CDS             complement(4980..5297)
                     /locus_tag="VQ508_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2490373.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5294..5593)
                     /locus_tag="VQ508_001390"
     CDS             complement(5294..5593)
                     /locus_tag="VQ508_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2490374.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5878..6069)
                     /locus_tag="VQ508_001391"
     CDS             complement(5878..6069)
                     /locus_tag="VQ508_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2490375.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFSFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6230..6904)
                     /locus_tag="VQ508_001392"
     CDS             complement(6230..6904)
                     /locus_tag="VQ508_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490376.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            complement(6910..7356)
                     /locus_tag="VQ508_001393"
     CDS             complement(6910..7356)
                     /locus_tag="VQ508_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490377.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7470..7973)
                     /locus_tag="VQ508_001394"
     CDS             complement(7470..7973)
                     /locus_tag="VQ508_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2490378.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7970..8332)
                     /locus_tag="VQ508_001395"
     CDS             complement(7970..8332)
                     /locus_tag="VQ508_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET2490379.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8398..10257)
                     /locus_tag="VQ508_001396"
                     /pseudo
     CDS             complement(8398..10257)
                     /locus_tag="VQ508_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10274..10388)
                     /locus_tag="VQ508_001397"
                     /pseudo
     CDS             complement(10274..10388)
                     /locus_tag="VQ508_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            complement(10515..10745)
                     /locus_tag="VQ508_001398"
     CDS             complement(10515..10745)
                     /locus_tag="VQ508_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2490380.1"
                     /translation="MDKPMLVFKRFGHQTYLMVQKEAKRCGIEFMGGPQGQVVRFWDN
                     REKNQDLVLIKDIEQELKITKSVASNLVKRIV"
     gene            complement(10869..11585)
                     /locus_tag="VQ508_001399"
     CDS             complement(10869..11585)
                     /locus_tag="VQ508_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2490381.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ508_001400"
     CDS             complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ508_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2490382.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13892..14224)
                     /locus_tag="VQ508_001401"
     CDS             complement(13892..14224)
                     /locus_tag="VQ508_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490383.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14295..14903)
                     /locus_tag="VQ508_001402"
     CDS             complement(14295..14903)
                     /locus_tag="VQ508_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490384.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14916..15374)
                     /locus_tag="VQ508_001403"
     CDS             complement(14916..15374)
                     /locus_tag="VQ508_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2490385.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15499..>15592
                     /locus_tag="VQ508_001404"
     CDS             15499..>15592
                     /locus_tag="VQ508_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2490386.1"
                     /translation="MNYEASKQLTDTRFKLLVGVQRTTFKEMLAG"
BASE COUNT         4489 a         3598 c         2857 g         4648 t
ORIGIN      
        1 ttgatatgag aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc
       61 ttaacttgaa ccaggtcaat attctgggct tgggctgcca tagacgcagc cacaataaca
      121 ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt
      181 cttgaaatcg ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct
      241 attgatggtt tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa
      301 gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca
      361 acggtcgcca ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg
      421 ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgatcattaa aatcactaac
      481 aaatttcttg atgacataga cccgcttatg gtattctact ttgggatgga tattagaatt
      541 ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca
      601 aggggcacag caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg
      661 taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag
      721 aatttcttct acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt
      781 tagaagagct caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct
      841 ccttttgtgt tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcata
      901 cttggatcca ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct
      961 gttgtcaaaa tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta
     1021 actggatcaa tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat
     1081 cctataccaa gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca
     1141 ttgtgagcaa tttgacgaac gggttcttcc aaagcacgga gaacaatatt acgtcctgtt
     1201 gcttcatctc ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt
     1261 ccaccacctg caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca
     1321 atgcggagtt tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct
     1381 acaccacctg acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt
     1441 gtagtttcga tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct
     1501 gcaccttcta caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga
     1561 gcttcaattg tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct
     1621 gttaagatgg cgatatcttc aagcatggct ttgcgacggt caccaaaacc aggtgccttg
     1681 actgctacta cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg
     1741 ccatccacat catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa
     1801 agtggcaaga tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt
     1861 tcaaggtcag ccaccatttt ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg
     1921 tcaaactgca ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg
     1981 gtgatgacac cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt
     2041 tcagaacgag aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg
     2101 gcgttgtttt tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata
     2161 ccgattggat ttgcacctgc tgtgacgttt ttgattcctt cacggacgat tgcttgggtc
     2221 aaaacagttg cagttgtagt tccatcacct gcgatatcat tggtttttga agccacttct
     2281 gataccaatt tggcacccat attttcaaaa tggtcttcta attcaatttc tttggcaata
     2341 gtcacaccgt cattggtaat caatggtgag ccaaatgatt tttcaagaac gacattgcga
     2401 ccttttggtc ccaaggttac tttaacagtg tctgcaagga tatcgacacc acggaccata
     2461 gctgaacggg catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat
     2521 tcttcaatga ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct
     2581 ttgacatcaa gacctgcgtg ggcttcaact aagacacggt ctccagtttt aacacttgga
     2641 gcaaccaagt caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgggctgtt
     2701 ttggtttttt cttgggctga acctgcgaga acaaagcccc caacggtttg ttctttttct
     2761 tctattttta agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga
     2821 gatagatatt attagcgctc tttatacctg agtgctaatt catagttcta ttgtatcact
     2881 tagtcagaaa taatcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac
     2941 tcagactttt taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc
     3001 aagctgctca aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt
     3061 tcgaagagta ttaaaatggc aattcttcct cttccaagac taaatctgcc aaatcttggc
     3121 ctgcattatt ttcacgcatg gcacgttgag cacgactttc caagagttgg aatcctgtta
     3181 caagtacttc ggtcacgtag ttcatttggc catttttctc aaagcgacgg gtacgcaatt
     3241 ctccatcaac ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc
     3301 tgccccatag gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac
     3361 gacggttcac agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt
     3421 ctggtgtaga cgttaaacgc ccaatcatga taactttatt atacatattt tcttcctcct
     3481 acttatctat tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgaggaaat
     3541 ttcttatttt ttatgaatca tgaaacctgt cgcctgttga ttggccataa tggtcatatc
     3601 tgtaatctga acacggcgag gctgactggt cacatagact actgtgtctg caatatcctg
     3661 agcttgcaag gcttctattc cttggtaaac ggacgcagct cgctctttat caccatgaaa
     3721 acgaacagtt gagaaatctg tttcgacaat cccaggctga atggttgtca ccttgatatc
     3781 cgttgcgatg gtatcaattc gcagtccatc cgaaaaggtc ttaaccgcag ccttggtagc
     3841 tgagtaaaca gcggcaccag cataggcgta gattcctgcg gttgacccca tattgataat
     3901 atgaccttga ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc
     3961 tttaacattg gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc
     4021 caagtcaaga gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag
     4081 aatatcagag caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca
     4141 gacggtttga tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat
     4201 ccgtcgtcct gttagaacga catcctcacc ctgctccaga taagcacgcg caatcgcttc
     4261 accgattcct gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat
     4321 cagatgctga caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca
     4381 acttggtctt ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat
     4441 tcacgaggac tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg
     4501 aaattccctt ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc
     4561 gcattaggtg ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag
     4621 atgttgaggt ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac
     4681 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc
     4741 aaacgagcga cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag
     4801 ctcgaaacct caaaaatgtt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4861 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4921 ataaggacat cactgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4981 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     5041 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     5101 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     5161 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     5221 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     5281 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     5341 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     5401 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     5461 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     5521 caagagcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     5581 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     5641 caagtaaaaa gcctcttcct ttaaggaaaa ggacttctta tactaagagc aaactagaaa
     5701 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5761 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5821 gtaaccatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5881 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5941 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaagga
     6001 aaaaaaactg tccaatatca ttcgtttttt aaaagcattc gacactacat catctacgct
     6061 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     6121 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     6181 atttcctact cttagtacgc ctattttgat ggttgaagtg aagatttttt tatttagcgt
     6241 agacaatttc caacttccca aaagccacat ctccacggat aatcaaggtt ttgctggttg
     6301 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     6361 gccagtgttg tggaacatag acggttgcat tcccgaaggc cacttcaata tttaaagttg
     6421 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     6481 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     6541 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     6601 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     6661 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6721 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6781 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6841 tcccttgcag caagatccaa gctgctaaaa ccaataaaac aataccaaat gctttctttt
     6901 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6961 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     7021 gtccactgag taaatctgac ggatgtttgc gatcgttgac ttggaaactc gattaaaata
     7081 gcgaggcaag atagactcca actcatagag cttgaggcga acctcataag cttccttctg
     7141 gttatgagcg tagatcttgc tcccttctgt ttcaaagaag agaatttctg acaggtctag
     7201 ataatattca cctgtcccct tgtaaaaagt caacctcgga gcctttgact cttgcaagag
     7261 ccgttgcaag tctgcaattt catctgtcaa agtgggggtc ttgatgacaa tttcagtctc
     7321 ctctaaattg ccgtcaatct caattcgtaa cttcatcaca tctcctttct gactctacta
     7381 tatcaaagct aagttaggaa tacaagggca aaaaagcaag tggtcaattt gaagcagtaa
     7441 gtggttgtag gactagctta acttacatgt cagaatagga agaaaaggaa tccccacacc
     7501 aaaccacaag catagccaac caagacatcc ttgggataat gaacaccccc taaaaccctc
     7561 accaacccca gcaaagctga aaagatcaag catactaagc caataggtag actagcatgc
     7621 agacaggcca tggagatgat agttgccgaa aagacatggc gactgggcat cgaccgtccc
     7681 gaactatcct tatcaagaag gggacagata tcccaagttt cataaggtct cgggtgattg
     7741 attttcttac gaaacaggga caaggccaca aaacctgatg caggaataag cacataaacc
     7801 ccaacttgct gaccaagtcc tagttgcagg taagtggtga ctagcaagat cagatagacc
     7861 aggggcatgg cgactgtcat caaacgattg aaactgcgta ataggaataa aagaattgga
     7921 cgactagtta gtctggagct gatatttcga taccattctt gataattttt catttatgct
     7981 tctcatactt ttctagaact tgttgagatg ttttcccctt ggcaagccaa tccactagct
     8041 tgtccaagcg acgtatttcc tgcatcagag gatcatcaat ttcctcaata cggacaccgc
     8101 aaatcttccc tgtaattgca attcgttcag ggacataata tggtgcttgt ctaaaaaaat
     8161 ctccgtaagt aacatcccta tccagacaag caagaacatc actcacttca taccctgtca
     8221 accaactggt cacttggtga actgactctg cctttccacc ctttcgctca acttttgcaa
     8281 tcaaggctaa gtagacagca gcaaatttca tattgtataa tttctgactc atacttctct
     8341 ctttctatgt gtactagtat aaataagaaa aagaaggccg tcaagccttc ttttgattta
     8401 ttcttctgct tcatcttctg taaattgact attgtacaag tcagcgtaga agctaccttg
     8461 cgccatcagt tcctcatagt tgccttgctc gatgatattt ccatctttca tgaccaagat
     8521 caagtctgca tttcggatgg ttgacaagcg gtgggcaatg acaaaggatg tgcgtccttc
     8581 catcaaacgg tccatggctt tttggatcaa ttcctctgtc cgtgtgtcaa cagaagaagt
     8641 cgcctcatcc aaaatcaaaa gcggtgcatc cttaagaagg gcacgagcaa tagtcaatag
     8701 ttgtttttgt cctacagaca aggtcacggt gtcatccaag atggtatcat agccatctgg
     8761 caaggtcata ataaagtggt gaattcccac agccttacta gcttccatca ttcgttcatc
     8821 actaatccct atttgattat agatgagatt gtctcgaata gttccttcaa agagccaggt
     8881 atcctgcaag accattgaaa aggcatcatg cacttctgaa cgcgtcatag ccttggtatc
     8941 cacaccatca atgtgaatac ttcccttatc aatctcatag aatttcatca aaagattgac
     9001 aatggttgtc ttaccagtcc cagtcggccc aacaatggca accttttgac cagcatgagc
     9061 tgtcgcagag aagtcatagt cttgaacatt gacaccgtcc accagaattt ctcctgctga
     9121 cacgtcgtag aaacgtggaa tcagattgac cagagttgat ttaccagaac ctgttgaccc
     9181 aataaaggcc actgtttgac cagtttctgc tttaaagcta acatgttcaa taactgcctc
     9241 cgaatttgcc gcatagcgga aggtcacatc cttaaactcg acctgacctt tgaagttttc
     9301 atcagtcagc tgcacttgaa cagggttttg gatagaagaa tgcaaatata aaacttgatt
     9361 aatccgctta gcagagacca tagttcgggg aagaacgatg aagagtgctc ccatgagaag
     9421 gaagcccatg acaacctaca tggcataaga catgaaaaca atcatgtcac taaagagagg
     9481 cagacgcgct atcggagcag cgtcgttaat cacataggcc ccaatccagt aaatcgccac
     9541 actcaaacca cttgaaatcc ccatcatgat aggattcaaa atagccataa gacggttgac
     9601 aaacaaattc aaacgggtca attcatcatt tactgctgca aatttttcat tttgataatc
     9661 ctctgcattg taggcacgaa cgacacgaat acctgttaaa ctctcacgag tgatactgtt
     9721 cagtttatct gtcagcccct gaatcaagga ctgttttgga aaggctagcg tcatcaaaac
     9781 ggtcgtcatc aggacgttga caatcactgc cacaagtacg gcccagagcc agtattctga
     9841 atgacctaaa atcttcccaa tagcccagat agccataatt ggaccacgcg ttaccacttg
     9901 caagcccata gtaatcaaca tttgaacttg agtaatgtca ttggtagtac gcgtcaagag
     9961 gctaggaatt gaaaatttct taatctctgt ctgcgagtaa tccaaaactc ggttaaaaat
    10021 atcacttctc agcctactaa tataaaaagc cgccactcgg gatgcaaaaa atccaactgc
    10081 aactacggac aagaaggcaa gaaagggcat tcccatcatc atgcttgccg gctgccacaa
    10141 ctcatctaaa ttagttcctt gactacctag caaatccgta attttcgaga tataggtcgg
    10201 cacttccaac tctagataga ccgaaaagca agtaaagaga atggctagta aaatcatccc
    10261 ccattctttt ctactaattc ttttggctaa tttctttatt ctctcctcct attcccttga
    10321 tattttgcct gtagttgacc gagaactttc tcaaaaatca gtaattcatc ttcatcaatg
    10381 tcttccatca actgcttgtc tatgcgttca aaaaaagcct taacctgttg catctgagaa
    10441 cgtgctttgt ccgtcagacg aacaaactta gcccgcttat caacaggact cgcctccaat
    10501 tccaccaaac cattttacac tatacgctta accagattac tagcaacaga cttggtaatt
    10561 ttgagttcct gctcgatatc tttaatcaag accaagtctt ggtttttctc gcgattatcc
    10621 caaaaacgca caacctgacc ttgcggccca cccataaatt caatgccgca acgtttggct
    10681 tccttttgca ccatcaggta agtttgatga ccaaaacgct taaagactaa catcggttta
    10741 tccataatct cccccttcta aataaaaata gttctctgga gaataattaa atttctatga
    10801 gaactatttt cttgattaaa aaaatcccaa gtgattttct cacttaggat catgttctat
    10861 aggttaaatt aaaacccatc tacgttcgta taaatctttt ggacgtcttc gtcgtcttca
    10921 agaacgctgt aaagtttttc aaaagtttca aggtcttcgc ctgacaattc cacttctgac
    10981 tgaggaatca tttccaattc agtcacttgg aattcttcaa taccagactc acggagggca
    11041 acgatagcct tgtgaaggtc agttggcgct gtgtaaactg tgattgtacc ttcttgtgct
    11101 tctacgtcat ccacatccac atccgcttcg agcaattgct caaagactgc gtctgcatct
    11161 tcacctccaa atacaataac acctttgttg tcaaagaggt aagaaacaga acctgaagcg
    11221 cccatgtttc cgccgttttt accaaaggct gcacggacat tggctgctgt acggttaacg
    11281 ttagaagtca aagtatccac aattagcata gagccatttg gcccaaaacc ttcgtaacgt
    11341 ccttctgtaa aggtttcgtc tgtgtttcct ttggctttat caatcgcttt atcgataacg
    11401 tgttttggca cttgggcttg tttagcacgg tcgataacga atttcaaagc tgagtttgat
    11461 tctggatctg gatcaccttt tttagctgct acatagattt ctacaccaaa ttttgcatat
    11521 actttagagt tggctccatc tttagccgtt ttcttggcta cgatattggc ccatttacgt
    11581 cccattagga atctcctttt ttcacatttt aatctttctt attataacac aagttttttt
    11641 gattttcact agaggaaatg gattttatta gcaaatcaag ctaggatagt actttacctg
    11701 ctaagatggt cttgcctttc tatctttatc aacagacact catccacatt caaaaaacaa
    11761 actagaccat tatctgcaaa tagaaagttt cagccaagtt tgacaaagtc agctcaaatt
    11821 actgtttgaa gtttgtagat ataagcgaca aaaacaatca tactgcacct tttgttgaca
    11881 gtctactcca gacatatcat agttccagta aatactttgc aatccaacag ttcttatagg
    11941 cgctattgta ttctaagaaa ttaatagacg agtttctaag caaacctcta atactcaata
    12001 aaaatcaaag agcaaactag aaagctagcc gcaggttgct caaaacactg ttttgaggtt
    12061 gcggataggg ctgacctggt ttgaagagat tttcgaagag tataatttac gtgttcccaa
    12121 gatggagaag ttagactagt acactggcac ttctaaacca ttgctagcaa ttgatttgtt
    12181 catatttaat ttcatttctt ccataaatgg gtattagata taaacagcaa aatatttccg
    12241 atacgtgtcg ttcttgaatt tccaatcatc taaaacaagt aaaggataat caatcccctg
    12301 tatatcaagg aattggctac cctttttact tttttacaca ttctgtttga tagattcatt
    12361 ttaacatcac gagcatactc caatggaaat cgctaggcaa gagataaact ttcagatatc
    12421 cgcagagaga tcatcgcctc tttttgtcgc aagcattctc ctctcctagt cattttctac
    12481 cttatcttct acctgaggat agagagttgt tccccaaata gaaatcgtcc gcttacgcac
    12541 tagtggcaaa tcggtttttt cataaaccgt acgccaccat tcccaggcaa gcccggtaca
    12601 ctctctaatt ttgacagaga gattacgaac attccctttt aaaggaatac tagtggtaaa
    12661 gtgagccgtt aaatcctgcc catttctgtc ccaagcctta ggagtcaaga cttccttacc
    12721 ttgatgatca taggataatt cattccaagt aatataatat tgggcaacat aggcaccact
    12781 atgatccagc agtaaatctc cgtttctgta agctgtaacc ttagtctcaa cataatctgt
    12841 actattttga aaggtcgcaa ctacattgtc acgtaaaaaa gaagttgtat aggaaatcgg
    12901 caagcctgga tgatctgctg taaagcgact gccttcttga atcaagtcct ctaccatatc
    12961 caccttgcct gttacaactc gggcacccga acttgggtcg ccccctaaaa taaccgcctt
    13021 cacttctgta ttgtccaaaa tctgcttcca ctctgtctga ggagctacct tgactccttt
    13081 tatcaaagct tcaaaagcag cctctacttc atcactctta ctcgtggttt ccaacttgag
    13141 atagacttgg cgcccataag caacactcga aatatagacc aaaggacgct ctgcagaaat
    13201 tcctctctgt tttaaatcct ctaccgttac agtatcttga aacacatctc ctggattttt
    13261 aacagcgtct acgctgactg tataataaat ctgcttaaaa ttaacaatct gaatctgctt
    13321 ttcgcctgaa tggacagagt taaaatcaat atcaagagaa ttccctgtct tttcaaagtc
    13381 agaaccaaac ttgaccttga gttgttccat gctgtgagcc gtgatttttt catactgcat
    13441 tctagctggg acattattga cctgaccata atcctgatgc cacttagcca acaaatcgtt
    13501 taccgctccg cgaacacttg aattgctggg gtcttccact tggagaaagc tatcgctact
    13561 tgccaaacca ggcaaatcaa tactataagt catcggagca cgatcgaccg caagaagagt
    13621 gggattattc tctaacaagg tctcatccac tacgagaagt gctccaggat agaggcgact
    13681 gtcgttggta gctgttacag aaatatcact tgtatttgtc gacaagctcc gcttctttct
    13741 ttcgataaca acaaactcat cgggtagctg attaccctct ttgatgaaac gattttcaat
    13801 actttctccc tgatgggtca agagtttctt tttatcgtaa ttcatagcta gtataaagtc
    13861 atttactgct ttatttgcca tcttctacct cctaataagt tcctggattg agttgcataa
    13921 actcagactt gttcagcgaa atcagccgtg gttggactaa gtaatccaaa atttcctcgt
    13981 acaattcttc tgagacattg cgtcgccgtc tggctagata agaagtcgga atgaccgtat
    14041 tatccaacat aaatacctta tctaagtcaa tcaaggttgg tcttgtaaaa ggattacgag
    14101 ctagatctgg ctcttctatc ataaagttct tgaccaaacg tctggtcaag agagctggtt
    14161 tgaaggtctg atttttaacc aactctttgt ttttagtcat gctgttgtca atacagatat
    14221 acatatgatt cttcacagcc aaatcgctac taatagtcgg aaaaggcaaa taaagagcta
    14281 caacatctcc tctcttaatc aagcaagagc accccctttt ctcctaatgt aacatagaca
    14341 ggattgacca agtcttctga ttgactcaga atttccaaag tttgagtttg gcgcgctgtc
    14401 aatttagtag catcttgtct cttcaataca aaatgcttgt cgccaataac cttgacaata
    14461 taatccttct ccaaagctga ctggtaaatc cacatcagat gttgtctgtc ctgagaactc
    14521 aagagagaag gattttcaag cctcccgata gtctgataaa aatcaaaaac aggagctaac
    14581 tcctgccaat ctgattggct agttgtcaag gctagaaaaa gggctttgcg agctgatact
    14641 tcttggttag ccttgagagt tactttcccc tccaagtttt ttagaaatcg ggaaactcca
    14701 gaaagcaaat ttttctctaa ctgcgagaaa taaaaacctt tcgttcccag acataagtct
    14761 ttcatgtcgc tttctctagc aaataagagc tcaaacattt gatagtaaaa gaaaaatatc
    14821 tggcactggg tcgcgctcat cttttcctta tcggcttctt tttttaacca gagcaagggc
    14881 gacaggtagc tggattgaga catttcctct acctcctact cttttttaac tggagcatct
    14941 gcactagctg ccacttcttt tgactggata ctttcccact ggttaatctc ctctgagata
    15001 agaccttcgc atgtcttgac aaatagggca aaagccttgg tctttcctgc atatttctcc
    15061 gtttggcatt gatagaggaa tttttctttc tccaggagtt gcgcagtttt ttggtaagaa
    15121 atccaatttt cctttgcatt atacaaattg ataatcccct cacacagcaa gccgagactg
    15181 gataaggcaa ccgaaatcaa acggtagcga tcacctggca taggaatagc acaaaagaca
    15241 gctatgagga aacctgccac gatttctgtt atttttaata ccttatagcg cctacgatgt
    15301 tgaacgcttt tctttaaaaa atgagctatc tgtacgtcta atcgctctgt caggtacatt
    15361 tcttctggcg tcatattcgt aactcctttc atttactttg ataatcatag tataacatat
    15421 ttgaatatca aagtatagta aaaactggac tataacttat tagacttcct gcgaaacaaa
    15481 atatggtata gtagttctat gaattatgaa gcaagtaaac aattaactga tacacgattt
    15541 aagctccttg ttggtgttca gcgtaccact tttaaagaga tgttagctgg at
//
LOCUS       DAVBCR010000048        15550 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCR010000048 DAVBCR010000000
VERSION     DAVBCR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.048"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     regulatory      79..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            382..1050
                     /locus_tag="VQ508_001405"
     CDS             382..1050
                     /locus_tag="VQ508_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2490387.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            1293..2075
                     /gene="thiM"
                     /locus_tag="VQ508_001406"
     CDS             1293..2075
                     /gene="thiM"
                     /locus_tag="VQ508_001406"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2490388.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            2077..2706
                     /locus_tag="VQ508_001407"
     CDS             2077..2706
                     /locus_tag="VQ508_001407"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2490389.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2915..3003
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3265..3825
                     /locus_tag="VQ508_001408"
     CDS             3265..3825
                     /locus_tag="VQ508_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2490390.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3826..5211
                     /locus_tag="VQ508_001409"
     CDS             3826..5211
                     /locus_tag="VQ508_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490391.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5213..5863
                     /locus_tag="VQ508_001410"
     CDS             5213..5863
                     /locus_tag="VQ508_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2490392.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5874..6566
                     /gene="tenA"
                     /locus_tag="VQ508_001411"
     CDS             5874..6566
                     /gene="tenA"
                     /locus_tag="VQ508_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2490393.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6571..7095
                     /gene="thiW"
                     /locus_tag="VQ508_001412"
     CDS             6571..7095
                     /gene="thiW"
                     /locus_tag="VQ508_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2490394.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            7095..7898
                     /locus_tag="VQ508_001413"
     CDS             7095..7898
                     /locus_tag="VQ508_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2490395.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            7891..8523
                     /locus_tag="VQ508_001414"
     CDS             7891..8523
                     /locus_tag="VQ508_001414"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2490396.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8732..9523)
                     /gene="thiD"
                     /locus_tag="VQ508_001415"
     CDS             complement(8732..9523)
                     /gene="thiD"
                     /locus_tag="VQ508_001415"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2490397.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9607..9705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9889..10284
                     /locus_tag="VQ508_001416"
     CDS             9889..10284
                     /locus_tag="VQ508_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2490398.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10295..10666
                     /locus_tag="VQ508_001417"
     CDS             10295..10666
                     /locus_tag="VQ508_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2490399.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            10676..12919
                     /locus_tag="VQ508_001418"
     CDS             10676..12919
                     /locus_tag="VQ508_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2490400.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13126..14901
                     /gene="spxB"
                     /locus_tag="VQ508_001419"
     CDS             13126..14901
                     /gene="spxB"
                     /locus_tag="VQ508_001419"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2490401.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            15012..15359
                     /locus_tag="VQ508_001420"
     CDS             15012..15359
                     /locus_tag="VQ508_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2490402.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         4586 a         2827 c         3432 g         4705 t
ORIGIN      
        1 tatatataaa gatatttttc ggtcaaatat cttgctaggg ctttcatttt ttgctatact
       61 ggttcagtaa ttaaataata gagacatttg gggtgcttta ggctgagata atacccattg
      121 aacctgatac agttaagact ggcgaaggga aatgtgaaac gttttttcgt atggcgaaat
      181 gaacgatcta atcttgtaag tcaactctaa ttcttgattg taattggagt tttttgttta
      241 tataaactgg ataggcttgt ttagttagct ctctgcaaat gctctcgatg tgctttaaaa
      301 ggatcaagtg gaattacgat tttgcttgat atttcttggt gttttattaa ttactaacaa
      361 gtaaagagag gagaagtaaa tatggaaaca caagactatg catttcagcc aggtttgact
      421 gttggagaat tattaaaaag cagtcagaag gattggcagg ctgcaatcaa tcatcgtttt
      481 gttaaggaac tttttgcggg gacaattgag aataaggtct taaaggacta tctgattcaa
      541 gattatcact tctttgatgc cttcttatcc atgctgggtg cctgcgtagc tcacgcagat
      601 aaacttgaat ccaagcttcg ttttgccaag caactaggct ttcttgaagc agatgaagat
      661 ggttatttcc aaaaggcttt taaagagtta aaagtagcag agaatgacta tctagaagtg
      721 actttgcacc ctgtaacaaa agcctttcag gatctaatgt attcggcggt ggcttcatca
      781 gactatgccc atcttttggt catgctggtc attgcagaag gtctctattt agactggggt
      841 tctaaagatt tggctttacc tgaagtctat attcattcgg aatggatcaa tctccacaga
      901 ggtcctttct ttgcagagtg ggttcaattt ctggttgacg aactcaatcg tgtcgataaa
      961 aatcgagaag atttgacaga acttcagcaa cgctggaatc aagcagtcgc tttagaatta
     1021 gctttttttg atattggtta cgatgtctag agaaggctca tgatatttac aaatttgatg
     1081 aggtaagact cttcgaaaat ctcttcaaac cacgtcagtt ctatccacaa cctcaaaaca
     1141 gtgttttgag caacctgcgg ttagcctcct agtttgctct ttgattttca ttgagtataa
     1201 atcattcaat gtaaatgaaa aaaattctta acgaaagata gaaataaatc gaaatcagta
     1261 gatcgtataa agtgaaaagg aaggatttca atatgacaag tttaaaatta ttaaaagaaa
     1321 aagcaccatt ggtcatttgt ataaccaatg atgtagtaaa aaatttcaca gcaaatggat
     1381 tagtagcact gggcgcctca ccagccatga gtgagtttcc agcagattta gaggatttgt
     1441 taaagtatgc tggtggttta ttaataaaca taggaacatt gacagatgaa aattggaaat
     1501 tataccaagc tgctctgaaa attgcagaga aatataatgt cccagcagtt ttagatcctg
     1561 tagcctgtgg agcaggagaa tatagaaaaa aagtagcaga tgatctaatc aacaattata
     1621 aactagcagc gattagagga aatgctggcg agattgcctc tttagtagga atagatgtgg
     1681 catctaaagg agtagatagt gcgggcgtag ataatattga cgaaattgct ctagcagcaa
     1741 atgagaagtt caatattcca atagtagtaa caggtgaagt ggatgccatt gctgttaatg
     1801 gagaagtggt aatgattcat aatggtagtg ctatgatgcc gaaagtcatt gggacaggat
     1861 gcttattagg agctgtagta gcaagcttta tcggactaga aaaaggtcaa gaattgaaat
     1921 cattagaaac agcaatgttg gtttacaata tcgctggaga aatggcagaa aaacgtccaa
     1981 atggacatct tcctgggaca tttaaagttg aatttataaa tgccttatac gagattacag
     2041 atgaagatgt aaaggaattc aaaagagtga agtaagatgt ttcataaaga attactaaaa
     2101 ctatatttta tttgtggaac gactacttgc caaggaaaaa atctatatac agtcgttgag
     2161 gaagccttaa aaggtggtat aaccttattt caatttcgag aaaaaggtga gggtgcttta
     2221 gaaggcttag aaaaacttga gctagctatc caaattaaag aactttgtaa aaaatacaat
     2281 gttccattta tcgttaatga cgatatagat ttagcaatgg aaattgacgc cgatggcgta
     2341 catgtaggtc aagatgatat tggtgttgat gaaattagaa aattgatgcc agataaaata
     2401 attggtcttt ctataagaaa cgaagaagaa tttcaacaat caaaagttga atatgtagat
     2461 tatgtaggtg ttgggcctgt atttggtacc cagtcaaagg atgatgctgg tggtactata
     2521 ggttatgaag gtcttgaatt gatgagaaaa ctattgccac aaatgccttt agttgcaatt
     2581 ggtgggatac agacgaaaca tattaaagac attataaaga ccaatatgga tggcgtttca
     2641 atcatttcgg caatatcgta tgcaaaaaat atagaaaaaa ctgttcggga aatgagtgaa
     2701 caatagatat ctatccttgg attctaagta agtaggattt tcctttttta aaaagctatg
     2761 tccagattca gcttgaatgg ctaattcatc aaacaggtca tttgtttttt gcaaaagaaa
     2821 gatctgctgt ctttgtgagt gcaaggctgt atttccagtt caatttcttg aactagtata
     2881 aaatgtgtgt tataataatc acataattaa ataataaaga catttggggt gctttaggct
     2941 gagatgatac ccattgaacc tgatacagtt aagactggcg aagggaaatg tgaaatgttt
     3001 ttttcgtatg tcgaaaagaa ctgtctaatc ttgtaagctg aactctaatt tgtatctgta
     3061 attagagttt tttcttttac gaaactggat gaagatgata tagtagattg aaataagatg
     3121 cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa tattttagtg ttcttgtgta
     3181 ctgttctaaa ttcagtctgc tatacaaagt atttaggtcg gtcttcttga gtgccccact
     3241 tttctttaaa aaaaggagtt ttttatgttg aaaaaatggc agttaaaaga tgttatcttg
     3301 cttgctttct tgtctatctt ttttggtggg gttttcgttg gttcaggata tgtgtataat
     3361 attctcagtc tactcttaac acctcttggt ttgcaggcct ttgccaatga aatcctcttc
     3421 ggtctctggt gtatggctgc gcccattgct gccatctttg ttccgagagt cggaagtgca
     3481 acgattggag aagtgctagc tgcgcttgct gaagtccttt atggtagcca atttggtcta
     3541 ggagctcttt tgtctggctt tgttcaaggt ttgggaagtg aatttggttt tatcgtaact
     3601 aagaatcgct atgaaagttg gctctctcta actgctaata gtattgggat tacgcttgtt
     3661 agctttgtct atgaatacat taagttaggt tactacgcct tttcccttcc gtttgtcctt
     3721 tccttgcttg tggtacgttt tatttctgtt tatttcttct gtaccatctt ggttcgtgcc
     3781 attgtcaaac tctatcatca gtttgcaact ggaggaaaag catagatggg tctggaacta
     3841 cgagcgattc agtccccaat cttctctgag ccgtttgatt ttacttttca tgcgcaagcc
     3901 tttaccttgt tagttgggag tagtgggtct ggaaaatcca gcctctttca aatgattgcc
     3961 caagttagtt ctcttcccta tagcggtcaa gtcctgatag atgggagcga ggtcagtcag
     4021 ctttctatcg tcgaacgtgt ccagacggtt ggtattctct tgcaaaatcc taatcatcaa
     4081 tttaccatgg agagcttgtt tgaggagttg gtttttacca tggaaaatat cggctatcac
     4141 cttcaggaaa ttgattctaa aatagcagag gttgtccagc aatgtcgttg caaggacatc
     4201 ttgcaccgtc tcatccatca cttatcaggt ggggaaaagc aaaaagcagc gctggctgtc
     4261 ctctttgcca tgaatcctag ggtctatctc ttggatgagc ccttcgcttc cattgaccgc
     4321 aagagcagaa tcgagatatt ggagattcta aaagagttgg tctatgatgg gaagacagtt
     4381 attttgtgcg accatgattt atctgactat aaagcctata tcgaccatat ggtggagcta
     4441 agagacggaa aactaaggga agtgtttcaa atcccttcct atgagatgac acaggttgct
     4501 tcaaaggaag ttgcttctag cccggaacta ttccatatga accgtgtgac tggtgagctt
     4561 ggtaatcgcc ccctcttttc aattgctgat ttcacattct atcaagggat ttcctgtatc
     4621 ctgggtgaca atggtgtcgg gaaatcaacc ctctttcggt ctattcttca atttcaaaag
     4681 tataagggga gcattacttg gaagggttcg gtcctgaaaa agaaaaagag tttgtatcgt
     4741 gatctgactg gtgttgttca ggaagctgag aagcagttta tccgagtcag tctgcgagag
     4801 gagcttcaat tagatggacc tgattctgaa agaaatcagc ggatttttca agctttacga
     4861 tattttgatt tggagcaggc agtcgataag agtccctatc aattaagtgg tggtcagcaa
     4921 aaaattcttc agctcctgac catcttgacc agtaaggctt ccgtgatctt gctagatgaa
     4981 ccttttgcag gtttggatga tagagcctgc cattattttt gcaagtggat tgtggaggag
     5041 aggaatcaag gaagaagttt tctgctcatt agtcatcgtt tagacccttt gatttctgtg
     5101 gttgattatt ggattgagat gactagtcag ggtcttcggc atgtgaaaga agtgaccatt
     5161 accaaaccac ttacatctca gagtagcaat acccaagggg aggtgagata gtatggtcaa
     5221 agtagcaacc cagacaccga ttatcagtct cttcttgctg attttatcct tggaaacatc
     5281 tttcattcct tcgattgctc tgaatctttc ggtagtcgca ttttgtattc tctttatgct
     5341 ctattaccgt cgatttaaaa tgttagcttg gatgatcata cttgccattt tcccatcttt
     5401 tgccaactac tgggcagttc agttacacgg agatgcttca caggcagtca tgcttggaac
     5461 gagggccttt gtgacagttt gtatcggcct tgtctttgtt tcctctattt cactaaaaga
     5521 gcttctcttg tacttggctc aaaaggggct atcacgctct tggtcctatg ccttgattgt
     5581 ggtattcaat tcttttcctc tcattcagca agaaatcaag tccctcaaag aagcttgcct
     5641 attacgtggt caaaaactac atttttggtc gcccttgatt tacagtaagg ttctgatgac
     5701 agtctttagg tggcgccatc tttacctgag agctctatct gcttacggat atgacgaaca
     5761 tgcacagttg aagaatagct atcggacttt ttatattcct aaaaaaacaa aattaatcta
     5821 cctgcttttc tttttattgc ttcaaaccag tctattttta taaaggagtt attatggaat
     5881 ttacagatat tgcgatggaa ttatccaaga aagcttggca ggcttccttt catcatccct
     5941 ttattttaca attacaagag gggaatttag aacctgccat tttccgctat tacctgattc
     6001 aggatgccta ctatctgaag gctttctcag aaatctatca tcttttggct gataagactt
     6061 caaaccaaga gatgaaaaga ctcttgaaac aaaatgctca gggtttagtg gagggtgagt
     6121 tatttattcg ccaacaattt ttcaaggaat tggaaatcaa cgaccaggaa atggagcaac
     6181 atccaatcgc tccaacctgt tatcattaca tttctcatat ttataggcaa tttgcagaac
     6241 cgaacttagc catagctttt gcaagcttgc ttccttgtcc ttggttatac catgatatag
     6301 gcaaatcact taatcttaaa ccatcaccaa atcctctcta tcaacaatgg attgaaactt
     6361 atattacgga tgagttagag caacagatca gagaggaggg agcactggtc aatcagcttt
     6421 atcgagaaag tgatgagaca gataagcaaa aaatgctaga tgccttccac atcagtgttc
     6481 atatggaagc caagttttgg gagatggctt accaacacca gacatggaag agcgatttac
     6541 agtctttaga aaaaggagaa gaatagaaac atgagaaagc accaactaca agttcacaaa
     6601 ttgaccattt tatctatgat gattgccctt gatgtagtcc ttacacctat ctttcggatt
     6661 gagggaatgg caccgatgtc cagtgtagtc aatattctag cgggaatcat gatgggacct
     6721 gtttatgcct tggctatggc tacagtgaca gcctttatcc gtatgacgac tcaagggatt
     6781 ccgcctttag ctctcacagg agcgactttt ggagcccttc tagcaggtct cttttataag
     6841 tacggtcgaa aattttactt ttctgccttg ggagaaattg tgggaacagg tattattggt
     6901 tctattgttt cctatcctgt tatggtgctc tttacaggat cggctgctaa gcttagttgg
     6961 tttatctaca ctcctcgatt tttcggagca accttgattg gtacagcgat ttcctttatt
     7021 gcatttagat ttttaatcaa gcaggaattc tttaaaaaag tgcaaggata tttctttgct
     7081 gaaaggatag actgatgcag gcattgacaa atccttttcc tataggctct agttccctca
     7141 ttcactgtat gaccaatgag atttcttgtg agatgctggc aaatgggatt ttggctctgg
     7201 gatgcaaacc tgtcatggca gatgatcccc gtgaagttct tgattttact aagcaaagtc
     7261 aggctctctt catcaatttg gggcatttgt cagctgagaa ggaaaaagca atccgcatgg
     7321 cagcttcgta tgcaaaccaa tcttctctcc cgatggtagt agatgcggtt ggcgtaacga
     7381 cttcatccat tcgtaagagc ttagttaaag accttttaga ctatagacct acggtcctta
     7441 aaggaaacat gtcagaaatt cgaagtcttg ttggattaaa gcaccacggc gttggggtcg
     7501 atgcgagtgc taaagatcaa gaaacggagg atttgcttca agtcttgaaa gactggtgtc
     7561 agacctatcc tggtatgtct ttcttagtta ctggccccaa ggaccttatt gtttcggaga
     7621 atcaagttgc tgtactggag aatggctgta ctgaattaga ctggataaca gggacaggag
     7681 acttggttgg agccttaaca gccgtttttc tcagccaagg aaagactggt tttgaagctt
     7741 cttgcttagc agtctcttat ctcaatatcg ctgctgagaa aatagttgtt caaggaatgg
     7801 gattggaaga atttcgttac caagtactca atcagctttc gctcctaaga agagatgaaa
     7861 attggctaga taccatcaaa ggagaggttt atgaatagag aagcacttag actatatctg
     7921 gtaaccaatc gctatcaaga ttccgtggaa agctttcttg caaaagttga gacggcctgc
     7981 cgttcagggg ttaccatagt ccaattgcga gaaaaaaatc tcacaaccaa tcaatattat
     8041 caactggcaa aacaagtcaa ggaaataaca gatgcctatc aggtcccctt gataatcgat
     8101 gatcggttgg atgtttgtct tgcggttgat gcagcaggtc tgcatatcgg agatgatgaa
     8161 ttaccagttt cggtagctcg caaagtcttg ggtcctgaaa aaatcctcgg tgtcaccgct
     8221 aaaacggtta aaagagctct ggaagcggag aaatcaggtg cagattactt gggtacagga
     8281 gccattttcc caaccaccac caaggaaaat gcgcccatta ctctgatatc gaccttgaaa
     8341 acaatttgcc aaacggttgc cattccagta gttgctattg gaggcttgac ttcggagaat
     8401 attgatcagc ttatgggcac tggcatagct ggtgtagctg ttgtacgtga tttgatgcaa
     8461 gcagaagata ttgaggcaaa aacgcaagcc tttttaacaa agttgcatga cattctttcc
     8521 taattaatac tcaatgaaaa tcaaagagta aactagaaag ctagtcgcag gttgctcaaa
     8581 gtactgcctt gaggttttag ataagactga cgaagtcagc tcaaaacagt gttttgaggt
     8641 tgcagatgga agctgacgta atttgaagag attttcgaag agtataaaaa ctctctaatt
     8701 cccttaaagt gggaactaga gagttttttt cttaatcttt aaaagttgta tggttgactg
     8761 gaccagaacc atgaccgagt tgaggggcat cttgaatagc ttttgtgata aaggccttgg
     8821 ccttatcaac tgcctggtaa agactcttgc ccttggctag ttcagcagta atcactgcag
     8881 caaaggtaca tccagtacca tgggtgtgac aggtttgaat tcgtgggctt tcccagacaa
     8941 attgttcatt cttggtaaag aggaaatctt tagcaccacc tttgagatgt ccgcctttga
     9001 taaccacaga ctgaggacca aattctttta aaatcaggcg accagcacgc tgcatgcctt
     9061 cggggtcatg gattgaaaaa ccaacaatct cttctgcttc aggaagattt ggcgtaataa
     9121 tagttgctag aggtagtaag tttgttttga gatagtctct agcacttgag tcaatcaagg
     9181 catctccact tgtagcaacc ataacaggat caaggacata gggacaatcc agttttttaa
     9241 gatagggttg gatgatttcc atgatttcag tagtagccaa cattccagtt tttacagcct
     9301 gaggtggaat atcagaaaag acactctcca attgggcttt caacatttga ggagaaacgt
     9361 gctcgattag ctgaacacct ctggtatttt gagcgacaag actggttaca acagccattc
     9421 catagacatc tctcgcttgg aatgacttta aatctgccat aatgccagca ccaccactag
     9481 ggtcagtccc tgcaatggtc aaagcaacgg gtaaataagt catctaaaac ctcccaacca
     9541 actgaagttt gtattcttaa gaacaagcta gtctgtttta gaaaggcaat agaagactgc
     9601 tagtccccat tatcatttcc acttccatca gctagcatta cctagattga gtcagaggga
     9661 tctaacagtc gttaatctca gctttacgca cccctagtgg ttgttttctt ttttctttag
     9721 tatagcatat ttttattgtt tatatagtaa tatttgactg caaatcgttt ttcaaatgat
     9781 taaaattcta tttttcgaag aaaatattat ttctataatt gacaaatgta gattttaaga
     9841 gtatactgat gagtgtaatt gacaaatgta gattttggag gtatggtaat gcagatttca
     9901 gatgcagaat ggcaggtcat gaagattatt tggatgcagg gggagcagac cagtacagat
     9961 ttgattaggg ttttggcaga gcggttcgac tggtccaagt cgaccattca aactcttttg
    10021 gctcgtttgg ttgagaaaga gtgtctgact cggaaaaaag aaggcaagtc ctttgtttat
    10081 tcagcccttt taactctaga ccaaagtcgg gatttacttg tccaagatat caaagacaag
    10141 gtttgttccc gtaggattag gaatttgttg gctgacttga ttgtagaatg tgaatttact
    10201 cagactgact tggaagactt ggaagctgtg atttcagaga agaaatcaag tgctgtaaca
    10261 gaagtaagat gtaattgtat gtaaaggaga cgtcatgtta aatagtattg taaccattat
    10321 ttgtattgcc cttatcgcgt ttatcttgtt ttggtttttc aaaaagcctg aaaaatctgg
    10381 acaaaaagcc cagcaaaaaa acggatacca agagattcga gtggaagtca tgggaggcta
    10441 tactcctgag ttgattgtcc tcaagaaatc agtgccagca cgcattgtct ttgaccgcaa
    10501 ggatccttca ccatgtctgg atcaaattgt ttttccagat tttggtgtac atgcgaacct
    10561 gccaatgggg gaagagtatg tagtggaaat cacgcctgag caggttggag agtttggctt
    10621 tgcttgtggt atgaacatga tgcacggcaa gatgattgta gagtaggtgg agactatgac
    10681 agaaattgtg aaagcaagct tagaaaatgg cattcaaaaa atccgtatcc gagctgaaaa
    10741 aggctatcat ccagcccata tccagcttca aaagggaatt ccagctgaga ttacctttca
    10801 tcgtgctact ccttcaaact gttataagga aattctgttt gaagaagaag gtatcttgga
    10861 accaatcggc gtagatgagg agaaagtcat tcgttttaca cctcaagaat tagggcgaca
    10921 tgaattttct tgtggcatga agatgcaaaa gggaagctat atagtcgttg agaagactcg
    10981 aaaatctcta tctctcctgc aacgtttttg gattactagt atctttactg tgcctcttgt
    11041 gattctcatg attgggatgt tgacaggtag cattagtcat caagtcatgc attggggaac
    11101 ctttctagca acaacgccta ttatgttagt tgcaggtaag ccatatatcc agagtgcttg
    11161 ggccagtttt aaaaagcaca atgccaacat ggataccttg gttgcgctgg gaactctagt
    11221 ggcttatttc tatagcctag ttgctctctt tgctggtctc cctgtttact tcgaaagtgc
    11281 tggatttatc ctctttttcg ttcttttggg agcagttttt gaggaaaaaa tgagaaaaaa
    11341 tacgtcccaa gctgtggaga aattactgga cttgcaagct aaaaccgcag aagtcttgag
    11401 tgatgatagt tatgtccaag ttcctttgga acaagtcaag gtaggcgacc tgattcgagt
    11461 gcgtcccggt gaaaagattg ctgttgatgg tgtcgtagta gaaggtgtct ctagtattga
    11521 cgaatccatg gtgacaggtg agagtctgcc tgtggacaag acagttggag atactgtcat
    11581 tggctcaacc atcaatcata gtggaacgct tgtctttaga gcagaaaaag ttggctcaga
    11641 gactgttttg gctcagattg tggattttgt gaagaaagct cagacaagtc gtgcgccgat
    11701 tcaggacttg acggataaga tttcagggat ttttgtccca gtagttgtca ttttaggaat
    11761 catgaccttt tgggtttggt tcgtcttgct cagggatagt gtggtcgtgc ttggagctag
    11821 ctttgtgtcc tctcttctct acggagtggc ggttttgatt atcgcctgtc cttgtgcctt
    11881 gggacttgca acaccgacag cccttatggt ggggacagga cgtagtgcca agatgggggt
    11941 tctcctcaaa aatggaactg tcttacagga aatccagaaa gttcaaactc ttgtctttga
    12001 taagaccggg actttgacgg aagggaaacc tgtggtaaca gatatcatcg gcgacgaagt
    12061 agaagtgttt ggattggcag cctccttgga agatgcttct caacacccac tggctgaggc
    12121 tatcgttaag cgagcgagtg aagctggact tgagtttcaa actgttgaaa attttcagtc
    12181 cttgcacggg aaaggtgttt cagggcgaat caatggaaaa caagttttac ttggaaatgc
    12241 taaaatgctg gatggtatgg atatttctaa tacttatcaa gataaactag aagaactaga
    12301 aaaagaagct aagacagttg tgttcttagc tgttgacaat gaaatcaaag gcttgcttgc
    12361 tttgcaagat attcctaagg aaaatgctaa gctagccatc agtcagctaa aaaaacgtgg
    12421 tctccgaaca gtcatgctga caggagacaa tgctggtgtg gcgcgtgcta ttgcagatca
    12481 aatcggaatt gaagaggtca ttgcaggcgt cttgccagaa gaaaaagccc atgaaatcca
    12541 taaactgcaa cagtcaggga aagtagcctt tgttggggac ggtatcaatg acgctcctgc
    12601 ccttagtgta gcagatgtgg ggattgctat gggagctgga acagatatcg ccatcgagtc
    12661 agcagatttg gtgttgacaa ccaataatct tttaggagtg gttcgtgcct ttgatatgag
    12721 taagaaaacc tttcatcgaa ttctactcaa tcttttctgg gcttttatct acaatgttgt
    12781 cggaattccg attgcagcag gagtcttttc aggtgttgga ctggctctca acccagaatt
    12841 ggcaggtcta gccatggcct ttagttctgt atctgttctg accagttccc ttctgttaaa
    12901 ttttagtaaa atagactaaa agtaatgact agatttcttt gttataaaac agaaatgaca
    12961 gaactttcaa aactgtatta gaatagagaa tagagagttt tgagcagatt tttagaaaag
    13021 tcagcataaa tatgatacag tggaatagta aaaatttgga gaacgtttcc aattctatgt
    13081 aatcgtattc tccaaatcta aaaaaattga aggagagtta tcattatgac tcaagggaaa
    13141 attactgcat ctgcagcaat gcttaacgta ttgaaaacat ggggcgtaga tacaatctac
    13201 ggtatcccat caggaacact cagctcattg atggacgctt tggctgaaga caaagatatc
    13261 cgcttcttac aagttcgcca cgaagagaca ggtgctcttg cagcggttat gcaagctaaa
    13321 ttcggcggct caatcggggt tgcagttggt tcaggtggtc caggtgcgac tcacttgatt
    13381 aacggtgttt acgatgcagc tatggataac actccattcc tagcgatcct tggatcacgt
    13441 ccagttaacg aattgaacat ggatgctttc caagagctta accaaaaccc aatgtacaac
    13501 ggtatcgctg tttacaacaa acgtgtagct tacgctgagc aattgccaaa agtaattgac
    13561 gaagcctgcc gtgctgcaat ttctaaaaaa ggtccagctg ttgttgaaat tccagtaaac
    13621 ttcggtttcc aagaaatcga cgaaaactca tactacggtt caggttcata cgaacgctca
    13681 ttcatcgctc ctgctttgaa cgaagttgaa atcgacaaag ctgttgaaat cttgaacaat
    13741 gctgaacgcc cagttatcta tgctggattt ggtggtgtta aagctggtga agtgattact
    13801 gaattgtcac gtaaaatcaa agcaccaatc atcacaactg gtaaaaactt tgaagctttc
    13861 gaatggaact atgaaggttt gacaggttct gcttaccgtg ttggttggaa accagccaac
    13921 gaagtggtct ttgaagcaga cacagttctt ttccttggtt caaacttccc atttgctgaa
    13981 gtttacgaag cattcaagaa cactgaaaaa ttcatccaag tcgatatcga cccttacaaa
    14041 cttggtaaac gtcatgccct tgacgcttca atccttggtg atgctggtca agcagctaaa
    14101 gctatccttg acaaagtaaa cccagttgaa tcaactccat ggtggcgtgc aaacgttaag
    14161 aacaaccaaa actggcgtga ttacatgaac aaactcgaag gtaaaactga gggtgaattg
    14221 caattgtatc aagtttacaa tgcaatcaac aaacatgctg atcaagacgc tatctactca
    14281 atcgacgtag gtaacactac tcaaacatct actcgtcacc ttcacatgac acctaagaat
    14341 atgtggcgta catctccgct ctttgcgaca atgggtattg cccttcctgg tggtatcgct
    14401 gctaagaaag acaatccaga tcgccaagta tggaacatca tgggtgatgg agcattcaac
    14461 atgtgctacc cagacgttat cacaaacgtt caatacgacc ttccagttat caaccttgtc
    14521 ttctcaaatg ctgagtacgg cttcatcaag aacaaatacg aagatacaaa caaacacttg
    14581 tttggtgtag acttcacaaa cgctgactac gctaaaattg cggaagctca aggagctgtt
    14641 ggattcacag ttgaccgtat cgaagacatc gatgcagttg ttgcagaagc tgttaaattg
    14701 aacaaagaag gtaaaactgt tgtcatcgat gctcgcatca ctcaacaccg tccacttcca
    14761 gtagaagtac ttgaattgga tccaaaactt cactcagaag aagctatcaa agccttcaag
    14821 gaaaaatacg aagcagaaga actcgtacca ttccgtctct tcttggaaga agaaggattg
    14881 caatcacgcg caattaaata attcctctcg ccgaaaatca aatatgaaac ttgtagactt
    14941 tattctttga ttttcataga gcagaacttt aaaagatcgg agcaattcgg tctttttatg
    15001 gaggagagta aatgaattta aatcaattag atattatcgt ttcaaatgtt ccccaagtct
    15061 gtgctgactt ggagcatatt ttggataaaa aggcagatta tgctgatgat ggttttgctc
    15121 agtttacgat tggtagtcac tgccttatgt tgtcacaaaa tcatttggtt cctttggaaa
    15181 actttcagtc aggaatcatt attcatatcg aggttgagga tgtagaccag aactacaaac
    15241 ggttgaatga gcttggtata aaggttttac acggaccaac tgtaaccgat tggggaacag
    15301 agtccttatt agttcaagga cctgctggtc tagtgcttga tttttatcgt atgaaatagg
    15361 tcgcgtagtc atttgacgtt aaccttagct atttttaaaa tagaaattga gaatatgaac
    15421 tattgaaaga tcggagcaat ccggtctttt tatataaaga aattgcatat aatactcatc
    15481 tgctttcaaa aagcattcta ttccatctcc gattaacgat ggactttatc acctccttct
    15541 ccagtccttg
//
LOCUS       DAVBCR010000049        15217 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCR010000049 DAVBCR010000000
VERSION     DAVBCR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.049"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            225..1907
                     /locus_tag="VQ508_001421"
     CDS             225..1907
                     /locus_tag="VQ508_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490403.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1892..2722
                     /locus_tag="VQ508_001422"
     CDS             1892..2722
                     /locus_tag="VQ508_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2490404.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2915..3418
                     /locus_tag="VQ508_001423"
     CDS             2915..3418
                     /locus_tag="VQ508_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2490405.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3472..3706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3871..4434
                     /locus_tag="VQ508_001424"
     CDS             3871..4434
                     /locus_tag="VQ508_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2490406.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4448..5074
                     /locus_tag="VQ508_001425"
     CDS             4448..5074
                     /locus_tag="VQ508_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2490407.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5186..6058
                     /locus_tag="VQ508_001426"
     CDS             5186..6058
                     /locus_tag="VQ508_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490408.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6188..6325
                     /locus_tag="VQ508_001427"
     CDS             6188..6325
                     /locus_tag="VQ508_001427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490409.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6749..7663
                     /locus_tag="VQ508_001428"
     CDS             6749..7663
                     /locus_tag="VQ508_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2490410.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7656..8669
                     /locus_tag="VQ508_001429"
     CDS             7656..8669
                     /locus_tag="VQ508_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490411.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8653..10245
                     /locus_tag="VQ508_001430"
                     /pseudo
     CDS             8653..10245
                     /locus_tag="VQ508_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10437..11340
                     /locus_tag="VQ508_001431"
                     /pseudo
     CDS             10437..11340
                     /locus_tag="VQ508_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11358..11495
                     /locus_tag="VQ508_001432"
                     /pseudo
     CDS             11358..11495
                     /locus_tag="VQ508_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11523..11864
                     /locus_tag="VQ508_001433"
     CDS             11523..11864
                     /locus_tag="VQ508_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490412.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11837..12148
                     /locus_tag="VQ508_001434"
                     /pseudo
     CDS             11837..12148
                     /locus_tag="VQ508_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12276..12863
                     /locus_tag="VQ508_001435"
     CDS             12276..12863
                     /locus_tag="VQ508_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2490413.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13253..14860
                     /locus_tag="VQ508_001436"
     CDS             13253..14860
                     /locus_tag="VQ508_001436"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2490414.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         4711 a         2374 c         3207 g         4925 t
ORIGIN      
        1 catacctacg gcaaggcgaa actgacgtgg tttgaagaga ttttcgaaga gtataaaatg
       61 atggggaagg ctgggcaact agtctttttc gatgtttata gattagttag gcaagtaagc
      121 taggatagga atctttttgg tgctaagatt ttaagagaag agactgcaag aagggcttga
      181 tttatgataa aatagaagtc taagaagcga atgaagagag taagatgaaa gaagctataa
      241 ttgagtggaa ggatttctct ttccggtatg aaacacaaca agaaccgacc ttgcaaggga
      301 tagacttgac catttacaag ggagagaaag tcttaattgt tggaccatct ggatcaggta
      361 aatctacctt gggtcagtgt ttgaatggga ttattcccaa tatttacaag ggtcagacat
      421 atggagaatt tttgataaag ggtcaaacag cctttgatat gagcatctat gataagtctc
      481 atctggttag cacagttttg caggatacag atgggcagtt tattggcttg tctgtggcag
      541 aagatttggc gtttgctctg gaaaatgatg tgacagccct agatgagatg aaaggtcgtg
      601 tttataaatg ggctgaaaag ctggaccttc ttcctttact ggatcagcgt cctcaggatt
      661 tgtcaggtgg acaaaagcag cgagtcagtc tggctggtgt cttgattgat gaaagtccga
      721 ttctcttgtt tgatgagcca ctcgccaatc tagatcccaa gtcaggtcag gatattatcg
      781 aattgattga ccagattcat aaggaagagg ggacgacgac tcttattatc gagcaccgtt
      841 tggaggatgt tctgcatcgc cctgtggatc ggattgtctt gataaacgat ggtcgtatcc
      901 tttttaatgg gagccctgac cagttgcttg cgactgattt attgactcaa aatggaattc
      961 gagaacccct ttatctaacc actctccgtc aattaggtgt ggacttagtc aaggaagaac
     1021 agttagcgaa tctggataac atgtctatct caaaaggtca ggttcagctg cagaatgaac
     1081 tggtaaaaga aactccagaa ttgcagtcac tctttaaatt agaggacgtg tctttctctt
     1141 atgatgatag accgatttta aaatccctac atttagatat taaaaagggt gaaaagattg
     1201 ctattgtcgg aaaaaatgga gcagggaaat caactctagc caaggctata agtagcttta
     1261 ttcagacgga aggacgctat ctttgggaaa aacaggatat aaaaggcgat tctgttgcag
     1321 agcgggcgga acgagtaggc tatgtgctac aaaatcctaa tcaaatgatt tcaaccaata
     1381 tgatttttga tgaggtggct ctagggctcc gtttgcgagg tgtggatgag aaggaaattg
     1441 aaacgagagt ctatgaaacc ttgaaaatct gtggacttta tgaattccgt aattggccta
     1501 tttctgccct gtcatttggt cagaaaaaac gtgtcaccat tgcttcaatt ttggtcttag
     1561 gagctgaaat tattctccta gatgaaccga ctgcaggtca agatcagaag aactatactg
     1621 agattatgga atttctcgaa gagttacatc gaaaagggca taccattgtc atgattaccc
     1681 atgatatgca attgatgctg gattattcag accgagtcct tgtcatggtg gatggagaat
     1741 tgattgccga tactgttcca gccagtctgt tgagcgatcc tgagctgtta gtaaaagcca
     1801 atctaaaaga aacctccatc tttaacttgg ctaagaaact agatgtggat ccactggatt
     1861 taacggcatt ttacaaagaa aggagagagg gatgcaagct aaattaatcg gttaccagca
     1921 tagagatact gtgattcatc gcttgtcagg agctgggaaa cttctctttt tcattctggt
     1981 atcattggcg gccatgatta gctatgatac cagaatgctc ttgctgattg ccatcttttc
     2041 agtctttctc ctctatttat cagaaattcg ctttaaagat gtttcctttg tagccgtttt
     2101 tgcgacggta tttgccgttt taaacgtttt gatggtctat ctcttttctc ccgagtatgg
     2161 ggttggactt tatggagaga gaagtgtgat ttggcaggga atcggtgtct acactctaac
     2221 cagccaggag ctcttttatc tgctaaattt ggccattaag tatctttgca ccattcctct
     2281 ggctattatc tttttgatga caacccatcc tagtcagttt gcttccagtt taaatcaaat
     2341 tggtgtgccc tataagattg cttattctgt cagcctgacc ttgcgctata ttccagattt
     2401 gcaggaagaa ttctttacta tcaagatgtc tcaggaggcg cgtgggatgg aattatccaa
     2461 gaaagcttct cttatgcaac gaatcaaagg caatctgctc attattacgc ccttgatttt
     2521 tagctcgcta gaacgcattg ataccattgc gactgccatg gagcttcgcc gctttgggaa
     2581 agaaaaaaaa cgcacatggt atagttatca ggccttgaaa aaaggagact atattacctt
     2641 gctcttggca accttgtttt tagtagctag tttactactt agcttgcaga atcagggacg
     2701 attttacaac ccttggaaat agcttgaaaa aattgaaaaa atcaagtcgt ttctattgac
     2761 aatgattctg aaagtgttat actaagaaag tagtttcgct gatttacttc aaacctgttg
     2821 tgaggtaagt taacgatgcc ttaaccacgc tgtttgctga gcttgactcc gggcagtgtg
     2881 gctatttttt tgcaatggtg aaaggaagca agtcatgaca aatcacattg tattatttga
     2941 acctcaaatt ccacaaaata cgggcaatat cgcgcgtact tgcgctgcga ccaattctcc
     3001 cctccacatc atcaagccta tgggctttcc tattgatgac cgcaagatga agcgggctgg
     3061 attggattac tgggataagc tagagattta tttttacgag agtttggaag atttcatatc
     3121 tcagatgaag ggaaaactct atctgatttc gaaattcgcg gaaaaagtct attctgaggt
     3181 ggatttatcg actgacgagg accattattt tctctttgga cgtgaagaca agggcttacc
     3241 tgaagatttt atgcgagaac atcctgagaa ggctctccgt attcccatga atgatgaaca
     3301 tgtccgcagt ctcaatgtgt ctaataccgt ctgcatgatt gtctatgagg ctcttcgcca
     3361 gcagaacttt gcaggtcttg aactcgtcca tacctatgaa gtggataaat tgaaataaaa
     3421 aatgaaaatg aacaaaatgc ttgcgcttgc aagcgttttt tgttatgata aaagagtctt
     3481 cagggcaggg tgtgattccc gaccggcggt gactttaact aggaaatgat cttttccttt
     3541 tatactttgt tgacaagctt tgcctaacca gaagttatgc ctacagcttg tcgcctagtc
     3601 taaaagaaaa atctctattt ccttctcttt aaagaagtcc gcgagcgcaa gctgatgtgg
     3661 tgagattcca caaccgacag tatagtctgg atgggagaag acgaaagaat agctttgtct
     3721 gttttgatag atttatagcc ggattgtaac cacttgcttt gtttttcttt aatagagtag
     3781 gagggaactt ttgggatata aaaagtgaga atagatagag gaatcctttc caacttcttc
     3841 tgattttata gaaaattgga ggaacctgtt atgacaaaca cacgtcgact ttcgaccatt
     3901 gcaattttat cagccatctc atttgtgctg atgtactttg actttccgct tttaccagcg
     3961 gcatctttcc tcaagatcga atttagtatc ttgccagtcc ttgtgggtct ggtggtcatg
     4021 gatttgcctg ctgctctagg agtgctcttg cttcgctcac tcttgaaatt gcttcttaac
     4081 agccaaggag tgaatactta cattggtttg ccgatgaata tcgtagcttt gggagttttt
     4141 gtcatcgtat ttgctttgat ttggaaaaag gaacggacaa cccttcgttt cctactaggc
     4201 tctctagctg ggactattgg tttaaccgtg gctatgttag ttctcaacta tgtttatgct
     4261 gttcctttgt acgctaagtt tgctaacttt gatattggaa aaattttggg actttccaac
     4321 tacctaatga ccatggtatt accttttaac ttgattgaag gtgtaatctt ttccgtttca
     4381 ttctggttgt tgtacattct cttgaaacca accttaaaac attatgaaag ataaacaaac
     4441 atttttaatg aagggcagtt ttgccctttt acttttcgtt attcttggct atatggtcaa
     4501 attttaccct gaaatgttgg tcaattttga ccaatcgatt cagactgcca ttcgaggaga
     4561 cttaccagat tatttgacta ttctttttcg atccctcaca cgtctgattg atattccagt
     4621 gattatcact tgggttgtca ttatagcttt tgtcttttat cgtaagcgat ggaagataga
     4681 aagtttcttc atgctgggaa atctgacttt ggcaggtctt ttaatcgtga cctttaaaaa
     4741 tatctaccag cgcccacgac cagctatctt acatctggtg gaggagaagg gattttcctt
     4801 cccaagtggg cattctctgg ctgtaacgct gatggtaggg actttgattg ttattcttag
     4861 tcagcggatt aaagatcagg tctggagaaa aatcgtacaa atcgtccttg gcctctacct
     4921 agtcagtgtg ctggtatcaa gagtctatct gggagttcat tacccatcag atgttcttgc
     4981 cagtctctgt gtgggcttgg gagccctgtt tatcgagttt cccttttatg ataagctccg
     5041 cttccaatgg cgatttaaag gcaaacagaa atgatggaaa agataaaaaa ttgtacaaat
     5101 acaaatagaa ccatatgaaa tatatacttg aaacatgttt tggagtttga tatattttaa
     5161 taaataaagc aaggaggaag aaagtgtggg tatgttatta gcgacacgct taaaaaataa
     5221 aagaaaggaa ttaaaattat ctcaaaaaga attagctgag gggatttgtg agcaagggca
     5281 gattagccgt atggagcaag gtaaatactc acctgggtca gaattactct ttcaactatc
     5341 taagcgattg aaggtgagca tggattattt ctttgaagat actgaggttt ctagtttaga
     5401 aaatattgat aagtttaaag aattatctaa gaaatttttg gatgagaggg aatatgaatc
     5461 tctccattac atctatgaac tggaaaaatc taaacgtgct cgtctatctg ctaaagacca
     5521 gatttatttg gattggattg atacattagt tcaattctgt tatttaaatc atcaaacagg
     5581 ggcagttgcg aaactggaaa aaatactgtt atctctgaaa aaatctgatt tttactatct
     5641 acaactggga aataccttgg ctaattttta ttttgatatg gggcaggaag acacatatca
     5701 ggaactctat caacttttaa aagagaacct ccagtcacta tctgttcatc atttggaaca
     5761 gttagaattg atgattaaat tccgatataa ctattgtaga tatttgtggt tgaatgaacg
     5821 aacatctgaa gctatagaag aaattacaga aacgatagct atttgtaaaa gatataatag
     5881 tagttatcga ttagcagatt tgtattgttt attgggaagt ataagcgagg gctttgctga
     5941 aagggatagg atactaggtt tttatcaagt atcttaccag ctttacaagc acgaagagaa
     6001 ccagagaatg atgctagaaa ttgagaagga aatgaaatta ttggaaagtg aagattaagc
     6061 aaaaagattt gtattcttag ataactttaa tacatattat aaaaaaaata aaaaaatatc
     6121 ataaaaatta ttgacaaacc aaaattgatg tgttagaata tcaccaatag ttaaggaggt
     6181 ttagaagatg aaaagatctc aagttgtcaa acgatgtata ttaattgctt tattgcttct
     6241 atttctactt aatgtgttaa gtggttcttt atctgtaaca actagaggag ttgatccaag
     6301 accagcatat ggagtttcgc tttaaagttg tgcatagttc ttcttttata gacggagagt
     6361 ttttctcaat agtaagtgat tcaaattttg atggatgatt ttccattaaa ttaagtgatt
     6421 gcgaaaactt tttgaaatac attctctttg gatattgaaa gtttgttttg gattttttta
     6481 tgaagcttat cttagctgtt agattggggt tttatgggat ttgtcctcac cgggaaaaaa
     6541 tcttaaatct ttgaagaaag aggtttttat cttatggtta gagatacatc agaagaaatt
     6601 gaagttattg acttgtcttc tatgaccgtt tcctactcat ctgttatgct aggaaactct
     6661 tggatgacgt ggaattgatt tattaagata gattatttgg tttttaccaa aaatctgtct
     6721 ttttaatttt taaaggacta atggtgatat gaaactatat caaataaatg atgggataca
     6781 aatttttgaa agtagtaatc ttgaaaaaat agtaattaga aattatagga atggtaagaa
     6841 aataactatc gatcaccaac cattattaaa aaaattacta ctattccttg aagttcctaa
     6901 acgtgaagaa gaaattctat tatatcttcc agaattatca aataaaacat tacgaagtgt
     6961 tttgacgaat tttatgagtt taaatataat tcattgtcaa gatacattaa taattcacaa
     7021 aaaaataaac atattgataa taggtttagg gactacgggt ggatatctgg cagatgggtt
     7081 atgtcgtagt aatattaatt cgaagctatt tcttttagat aaggatcgtg tagatgagac
     7141 taatgtaggt agacagattt atttaagaga tgatattggt caatataaag ttgatgtatt
     7201 taaacaaaag tttagtgata aagaaataaa ggtattcaaa atttttatta atagtaagaa
     7261 agatttggaa aagatttgca aaagggaggg tatcgatttg gttatacaat gtggtgactc
     7321 cccttcacct cgagaattag gaaaaattgt gaatacagta tgtgatactc tccagatacc
     7381 ttatatagtt aacactggct acatgagtaa tgtagttcct ttgccagaat tttattatcc
     7441 aaataataga tacaatttta attataagca tcaattttca gatgaaaagt tactgttctc
     7501 acaagttttg tctaaagctg attatatggt ggcaattcaa tcatcatttg taatgttaat
     7561 gcaaataaaa aattttatat ttaatgaaga acctatttat tatggttata ggggatacta
     7621 taattctgta acattagcat gggaggtgga aaaaattgac taaatattta gaattggatt
     7681 attctcattc atatattttg gaaggtttta gaaaaaatga tgcgtttatt gaaaatcaca
     7741 aagaatgcct tgatatgtta tcagataaga tttggcgaga taataaatat atgaatacag
     7801 aaaaaaacat atcatcaccg acacgaacaa tcttatcacg ctatacttgt aatattttat
     7861 cgtctttgtt tattgatatt agcaaaaata gcatagaaaa attgatagtg acatgtgaaa
     7921 atagcgatct taattggatt tataattatt ataaatcggt ttacaatgga gtattttgtg
     7981 aattgcgaca acttatacaa cagtactgta aggtgaagga taagtatgaa aagtggcttt
     8041 ttataaaagc tattataaat caaggtattc gtcaaaatga gaaagaagtt gttgaattct
     8101 ttttaaaaca attgaaggat aataatcaag gtatttttga ttacatcaat agttttagtt
     8161 tttatctatt aaagttttat tcaaaagaca aatctcgcat ttttttagaa gagcagctag
     8221 atatagatga ttttttggga tcagataaag aagattatgc taggtcttta gtttttaaga
     8281 attatgcttc tctattgcct gatacttctg aattaaaaag aaatattatg gaaaaatgtt
     8341 taagtcaaac acctcaagat acagatttat ggaaagaatg gtttgaaact tatgccagtc
     8401 agaaagagat tagacaaaaa gcaacggaaa tatttaagca tggttattca gatatttcgt
     8461 tactaaaatt agttaaaatt gagcctgatg acactgagag tttaatcaga atgattatct
     8521 tatgtaccag tgacctaaat agtaatattg caaggtattt gatttcattt ttgagcgatg
     8581 ggcgattaaa agaatttttg gagttatttg ttgaaaattt tgactttttg aatattattg
     8641 agggaaaaac tagtgagatt aatttttaga ataattagaa aagatattat attatattct
     8701 atattaacat ttttgctatc aatagcgatg gtaagcgaag catttttgat gcaatttatt
     8761 gttgattcaa ttaaatttgg tgaaaagaag tatgttttga tttctatttt tattgtaatt
     8821 tttttgattc ttcagacttt attgtattac tatcaacaat ttcttacagc tgtattaagt
     8881 aaaaaatctg catatgagta ccgtaaatta atttttaaaa atattcagca gacttctctt
     8941 aggcttgtta caggtgaaaa aaatgatagt attctagctt ctttgacaac gcaaatcgat
     9001 caaatagaat ataattattt ttattctgtt tattggggtg gatatttgct ttgccaatta
     9061 gtggtagcta tagttatgtc gctttatcta aatccattga tgtctgtatt aaccatttgc
     9121 ttaagtctcc ctaatttatt tgtagcactt ctttttaaaa actccttgga acaaaagcaa
     9181 gaagagctaa ttaatgagac aaattcctca atagcagtta ctcaagattt aattgaggga
     9241 attactgatt ggaaaattgc aaatagagaa cctaatatat ttagagtgtt tgaaagcaaa
     9301 acattgaagc ttttagagaa gcaagtgaag gtagaaaaat ttcaatacat tgttgttagt
     9361 ttaaatcaat tgttttcaaa tgttttatat tttggtagtt ggatagtaag tggattcttg
     9421 attatacggg ggcaactttc attagggagt ttgatagcgt tctctcaatt attagcaaga
     9481 atttcttacc ctgtttacgc ttcttaggat ttattgtcaa aatatattag cggtaaaaaa
     9541 attgtagaaa ttctttctaa agaatttata ttttcagacg aaagcagaaa ttatccagat
     9601 gagtttaatg ttatttcatt tgaacattat tttacaaaca ttgacgaaaa ggatatgcct
     9661 tttgataaag aattttacaa aaataaaaaa tatttgttga aagggaaaag tggtagtggg
     9721 aaaacaacaa ttttaaaatc gattttacga gagtatgatg attatagtgg aattgtaaaa
     9781 attaatgggg tagatgttag ggatataaaa gagagtgttc tttttgacaa tatagcttat
     9841 gtttcccaaa aacctcactt gttcagcgca agtcttagag acaacctgac attattttct
     9901 gaagacttca ctgataatga gatagttgag gttttaaaat ttgttgaatt atctaaatgg
     9961 gctaataaag attgcttgga tatgatgctt tcaaatgatg atataaaatt atcaggaggg
    10021 gaggtcaaaa gggtttcttt agctcgagcc ttattgatga ataagaatat tctgttattg
    10081 gatgagttct ctgctgggat tgattatgaa acattgttaa aaatagagaa gaaactcatt
    10141 gatttggata agatgattat ttacgtgacg catgtggata tagatagatc cggcaaacag
    10201 tttgatgatg tcattgattt ggattgtctt tttatatcct cgtgacatga gagttgagtc
    10261 agttgaatta ttttgtcact ctgtttagtt cttgttggta gtgtcaggta atctttgtta
    10321 atttttcagg aagtctattg aaatatcctc ttggttatat attacgagga gtaattaatg
    10381 gatattctcg agaattcaat acaacaattt gattctaaag ttaagtatgt ttctttattt
    10441 gcggaatcaa tcttttatca gataccatga gctattttgc aggtgctatg atttctcctt
    10501 tgtatgtgag gattatcgga gatgatatga cagatgctat gggggttaga caggcaactg
    10561 ctggtgtagt taatattctc agcaacctta tagggggagt tttgattggg ggtcatcagt
    10621 cttcagatgt ttgtaggttt gaatgctttg acctttcttt ttgcactgtt gggtgtggtg
    10681 tttattcgta cttctttgag agatattgag gaacagatgg aggttagtaa aagattagcc
    10741 gcaaaatctt ttggagagca tttcctccat tcaatgaaga tattgtcaag ttttccagcc
    10801 atcatcaaat taatattcat tgcagctatg aaccaagtga ttttaagcat tattatccct
    10861 ttaacgactt tgatgttaat ccatcatcca tttatcttct ttgacactgg gaaatccatt
    10921 gcagcattgt cagtggttat tttttcagga acgattgtgg gaagtttttt gagtggaggt
    10981 atcctaaaaa atattcccat aaaaatgatt gtctactttg aagaaattat gcatctctgt
    11041 attctctttt cttggttgac caatcatttc ctccttttat tggtagttgt cttcttatgc
    11101 gcagttgcag taggaatgat ggggcctcgg attttcaacc ttgtattctc gatggttcca
    11161 gaagaagtca tgggaaccat ccagtcagtg cttggagtat tcaatgttgt tgtaccagga
    11221 attcttagca tgctcatcgt tgccttagcg agtgctacaa acctaaaaat cacagtgctt
    11281 ccacttttta tttttgtatt gactgcctta gcaattgtga aagcaatgaa aatagagtaa
    11341 tagtagtatg aactactgtc tctttttggg tgggagttca ttacccatca gacgtccttg
    11401 ccagtctctg tgtgggcttg ggagttttgt ttatcgagtt tcccttctat gacaagctcc
    11461 gcttccaatg gcgatttaaa ggcaaacaga agtgagtatc gaattccctt gaggagaaag
    11521 aaatgaaagt caatatagca gatcttcatc cgactcaact atatttatca gaaaagaagt
    11581 tgcaagatat tcagatgctt taccagtcgg cagaaacaat ccaagtcgat ccaatcagta
    11641 ttcttgcctt tggagattgt ttgctgatta cagatgggca tcacagggct tatcaggctt
    11701 tattggcagg tcgggatacg atttctgctg agtgggatag agatggtggt gatgaactat
    11761 atcatctcta tgcgcaagct tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa
    11821 atcatatctt acctctttgg atcggcatgg tgagcttttt ttgattgaag tcaaggagaa
    11881 gggagaatgg tttccaattg gggatattac actatctcag gataatctcc ccattgtgat
    11941 tgggaattcc gcttaccaac atcgaggact tggaaaaaag attctaagtg ctttgattga
    12001 attggctcga gtaaaaggat ggaaagaatt gagagtcaag gaaatctaca cctacaatca
    12061 tgcttctagg aggtgtttca agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg
    12121 aaggagtttt atattggaat tagtctaatc ctgccacttt ctccccctta acctttgact
    12181 attccgcaaa attatcgtaa aataaagagt aaatgataaa atgaggtcag agtctgttcg
    12241 ctctgacgat agtagtataa atgaggagaa acgctttgga attagaagta tttgctgggc
    12301 aagaaaaaag tgaactatct atgattgagg tagcgcgtgc tatattagaa cttcgtggtc
    12361 gcgatcatga gatgcatttt agcgatcttg taaacgaaat tcaaaactac cttggaacat
    12421 caaacagcga tatccgcgaa gctttgcctc tgttctacac agagttgaac tttgacggta
    12481 gcttcatctc acttggggac aacaaatggg gtcttcgttc atggtatggt gtggacgaaa
    12541 tcgacgaaga aatcatcgct cttgaagaaa atgacgacga tgaagtagca ccaaaagcta
    12601 agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga tgccattgac tacaatgcag
    12661 atgatccaga agacgaagat gcatacgaag cagatccagc tctttcatac gatgatgaaa
    12721 atccagatga tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac gaaatcgctc
    12781 cagatgactt gggagaagat gtggatctca acgaagacga cgacgagttt tcagatgacg
    12841 acgctgaaac cagcgaggaa taagagttag ctattgacaa ttatcctatt tttaggtatc
    12901 atattgtttg ggcacctctt ttagaggtcg gggctcccta gttcttaggg agctattttt
    12961 gttttttcaa gaagttatct tcttgtattt tatactcaat gaaaatcaaa gagcaagcta
    13021 ggaaactggc cgtaggctgc tcaaaacact gttttgaggt tgtagataag actgacaaag
    13081 tcaggaacac atatctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag
    13141 tattagttgt gaatctggtg cagtcgtccc agattattct tattagtagg gtcttgtttt
    13201 ctatatcccc tcgtagttaa caagaccttg agcattttag aaagaggaat ctatgtctac
    13261 gaaatatatt tttgtaactg gtggtgtggt atcgtctatt gggaaaggga ttgtggcagc
    13321 gagtctaggc cgtctcttga aaaatcgtgg tctcaaagta accattcaaa agtttgaccc
    13381 ttatatcaat attgatccgg gaaccatgag tccttaccag cacggggaag tttttgtgac
    13441 agatgacgga gctgagacag atttggactt gggtcactat gaacgtttca tcgatatcaa
    13501 tctcaacaaa tattccaacg tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga
    13561 acgccgtgga gaataccttg gggcaactgt tcaagtcatt cctcatatca cagatgcttt
    13621 gaaagaaaaa atcaagcgtg ccgctctaac gaccgactct gatgtcatta tcacagaggt
    13681 tggtggaaca gtaggagata tcgagtcctt gccattccta gaggctcttc gtcagatgaa
    13741 ggcagatgtg ggtgcggata atgtcatgta tatccataca accttgcttc cttacctcaa
    13801 ggctgctggt gaaatgaaaa ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt
    13861 gggaatccaa ccaaatatgt tggttattcg tacagaagag ccagctggtc aaggaattaa
    13921 aaataaactg gcccagttct gtgatgtggc accagaagcc gttatcgaat cgttggatgt
    13981 tgaacacctt taccaaattc cactgaactt gcaggcacaa gggatggacc aaattgtttg
    14041 tgatcatttg aaattagacg caccagcagc ggatatgaca gaatggtcag ccatggtgga
    14101 caaggtcatg aacctcaaga aacaagttaa gatttccctt gttggtaagt atgtggagtt
    14161 gcaagatgcc tatatctcag tggtcgaagc cttgaaacac tctggctatg tcaatgatgt
    14221 agaagttaaa atcaattggg tcaatgccaa tgatgtgaca gcagagaatg tagcagaact
    14281 cttgtctgat gcggacggga tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg
    14341 gaaaatccaa gccatccgct atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt
    14401 gggaatgcag ttgacatgta tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa
    14461 ttctgcagag cttgcaccag aaacaaaata ccctatcatt gatatcatgc gtgatcagat
    14521 tgatattgag gatatgggtg gaacccttcg tttgggactt tatccgtcta agttgaaacg
    14581 tggctctaag gctgctgctg cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca
    14641 ccgttatgag tttaataatg ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc
    14701 aggagtttct ccagacaatc gtttggtaga aatcgtggaa attcctgaaa ataaattctt
    14761 tgtagcttgt cagtatcacc ctgaactgtc aagccgtcca aaccgaccag aagaactcta
    14821 cactgccttt gttactgcag cggttgagaa cagcaattag caaaatcaga acctttgaga
    14881 aaaatctcag aggttttttg catacgatga tattgcagta tatctgaggt agggggcctc
    14941 tgtatgtacc tgctaccgtt gaaatcaata gcgactccct cttgccctgt gctagtgaat
    15001 ggatttatca gtatattgaa atgaaataaa atttgaacaa attaattcgg aaagccaaat
    15061 caatttctag caaagtttta ggaactggat tgtatagtga attgaaataa gatgtgaaca
    15121 tctctatcag aaaagtcaaa ttaatttata gaaatatttt agcagtcaag atgtactgtt
    15181 atagattcaa tacattatac ttttttaatt taatcca
//
LOCUS       DAVBCR010000050        15053 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCR010000050 DAVBCR010000000
VERSION     DAVBCR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.050"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(72..620)
                     /locus_tag="VQ508_001437"
     CDS             complement(72..620)
                     /locus_tag="VQ508_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2490415.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(635..1483)
                     /locus_tag="VQ508_001438"
     CDS             complement(635..1483)
                     /locus_tag="VQ508_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2490416.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1671..3020
                     /gene="trkA"
                     /locus_tag="VQ508_001439"
     CDS             1671..3020
                     /gene="trkA"
                     /locus_tag="VQ508_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2490417.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3024..4463
                     /locus_tag="VQ508_001440"
     CDS             3024..4463
                     /locus_tag="VQ508_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2490418.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVVVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4589..6268)
                     /locus_tag="VQ508_001441"
     CDS             complement(4589..6268)
                     /locus_tag="VQ508_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2490419.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6338..7750)
                     /gene="lacG"
                     /locus_tag="VQ508_001442"
     CDS             complement(6338..7750)
                     /gene="lacG"
                     /locus_tag="VQ508_001442"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2490420.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7760..8104)
                     /locus_tag="VQ508_001443"
     CDS             complement(7760..8104)
                     /locus_tag="VQ508_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2490421.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8272..10158)
                     /locus_tag="VQ508_001444"
     CDS             complement(8272..10158)
                     /locus_tag="VQ508_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2490422.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10160..11482)
                     /locus_tag="VQ508_001445"
     CDS             complement(10160..11482)
                     /locus_tag="VQ508_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2490423.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11650..12873
                     /locus_tag="VQ508_001446"
     CDS             11650..12873
                     /locus_tag="VQ508_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2490424.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13172..13342
                     /locus_tag="VQ508_001447"
     CDS             13172..13342
                     /locus_tag="VQ508_001447"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490425.1"
                     /translation="MTKQIPKFTKDTAQLYTCKWLLYNKVTKMYDHTIVNHSVREYIT
                     DSISTNTIEEAE"
     gene            13954..14238
                     /locus_tag="VQ508_001448"
     CDS             13954..14238
                     /locus_tag="VQ508_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490426.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14231..14469
                     /locus_tag="VQ508_001449"
                     /pseudo
     CDS             14231..14469
                     /locus_tag="VQ508_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14565..14783
                     /locus_tag="VQ508_001450"
     CDS             14565..14783
                     /locus_tag="VQ508_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490427.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         4947 a         3112 c         2406 g         4588 t
ORIGIN      
        1 acaatttcaa aacactgttt tgagcaacct gcggctaact tcctagtttg ctctttgatt
       61 ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgggttcc tgcataggca
      121 agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc cgcaacaatt
      181 ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc aagtggtgcc
      241 aagacacccc aaacaagggc atttgcaagt agttgaatga ggttaaaaat aagaatatct
      301 ttccagtcaa aaacaccatt gatcacgcga acgtactttc taaaaagtcc cacaactaaa
      361 ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccaac aagagagtcc
      421 ttgattgcat gaccaatcaa cccaacaagc aaaccgataa tcggtccaaa aataatagaa
      481 agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg ggttggaatg
      541 ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac aacttgttta
      601 attgtaaatt tgatttccat actattctcc tattttatcc ttctattttc tttatttcaa
      661 tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct tggttgatag
      721 ccaaacctaa acgatagaga gagttgatgt aaaggatggg ttgcccaatt ctcacatctg
      781 caaatgattt gccatagaca acctgatttt gatagaccag catatcagca tgatagatgg
      841 tcacttcaaa acgatcacca aattctggtt ccaacttgta aaattcttcc cgtgtgatag
      901 aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc accagatgat
      961 cttctatgat ggtcgctacg actggaagct ctacaatctg ttccacactg agctctggcc
     1021 ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtatag gcatagacat
     1081 cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc acctcagaaa
     1141 tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc ccattatctg
     1201 gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt ttcgaaccga
     1261 cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc accgtctgaa
     1321 agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc aagtgatgga
     1381 tttttaaggt tggagactct tctaaagcca ctccaatcat agccgatacc gcaccatcaa
     1441 ccagaccaaa gtctgattgt aataccagta aattattatt cattttatct ccttgtatat
     1501 cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg gttagggtat
     1561 agttttatct tatagcgaat tttcactaaa agctcttgtt attagctagt aggtgcattt
     1621 tttcttgaaa aagggtatga tagttacatc atgaaaaagt aggttttatt atgaaaatta
     1681 tccttgtcgg agggggaaaa gttggttttg ccctctgtcg ctccttggtt gcagaaaagc
     1741 atgatgtttt gctgattgag caagacgaag ctgttctcaa tcatattgtc agtcgctttg
     1801 atatcattgg tatccttggt aacggggccg attttgccat tcttgagcaa gccagcgtcc
     1861 aagattgtga tatctttatc gccctgactg agcacgatga agtcaacatg attgcagcag
     1921 ttctagccaa gaaaatggga gctaaagaaa ctatcgttcg ggtgcggaac cctgaatatt
     1981 ctaactctta tttcaaggaa aagaatattc tcggtttttc tcttatcgtt aatcctgagc
     2041 tcttggctgc ccgtgctatc gcgaatatca ttgacttccc caacgccctg tctgtcgaac
     2101 gctttgctgg tggacgcgtt agcctcatgg aatttgtcgt caagtccacc agcggtcttt
     2161 gccaaatgcc catttctgat tttcgtaaaa aatttggtaa tgtcattgtc tgtgcgatag
     2221 agagggatca tcaaattatc attccaagcg gtgacatgac tgtacaggat aaagatagaa
     2281 tctttgtcac tggtaaccgt gtcgatatga tactcttcca taattatttt aaatcacgcg
     2341 ccgtgaagag ccttctcatc gttggggcag gtagaattgc ctattatcta cttggtattc
     2401 tcaaagatag tcgtatcgat acaaaagtca ttgaaatcaa tcctgaaatc gccagcttct
     2461 ttagcgagaa attcccaaat ctctacatcg ttcaaggaga tggaaccgca aaagatatcc
     2521 tgctggaaga aagtgctcaa cactatgatg ccgttgcgac tctaacagga gtcgatgagg
     2581 aaaatctgat tacatctatg ttccttgaca gggtaggtgt acagaaaaat attactaagg
     2641 tcaatcgtac cagtctcctc gagattatca atgcgcctga tttttcaagt atcatcacac
     2701 ctaaaagcat cgctgtagat acgattatgc actttattcg tggtcgagtt aatgcccagt
     2761 attcagacct tcaagccatg caccatctag ccaatggcca aatcgaaacc ctgcaattcc
     2821 atatcaagga agccaataaa atgactgcca aacctctttc tcaactgaaa ttgaaaaaag
     2881 gggttcttat tgcagccatt attcgaaagg gcaagactat tttcccaact ggggaggata
     2941 tgttggaagt tggagacaag ctcctagtaa caaccttgtt gccaaacatc accaagattt
     3001 atgacttgat tgcgaggtaa gaaatgaata aaagtatgat tcgttacctc ctttcaaagt
     3061 tacttttgat tgaagctgtt ctcctcttgg ttcctgtggc tatcgctgtc tattaccgtg
     3121 aatcgagcca agtctttaca gccctctttt cgaccatagg gattctcgta ttgttaggcg
     3181 gttcagggag tttacagaag ccaaaaaatc aacggattta tgccaaggag ggagtcttga
     3241 tcgttgccct ctgttggatc ctttggtctt tctttggtgg tctccccttt gtcttttctg
     3301 ggcaaatccc cagccttatt gatgcctttt ttgagattag ttccggcttt acgactactg
     3361 gagcaagtat tttgaacgac gtttcagttc tcagtcgttc cctcctcttc tggcgaagtt
     3421 ttacccactt gattggaggg atgggagtgc ttgtttttgc acttgctatt atggacaatg
     3481 ccaaaaatag ccacctagag gtgatgaagg ctgaggttcc aggtcctgtt tttggcaagg
     3541 ttgtatccaa actaaaaaac actgcccaga ttctctatct cctttatcta gctctcttct
     3601 ccctctttgt catcatctat tatctagctg gtatgcctct ctacgatagt tttgtcattg
     3661 ctatggggac agcgggaact ggaggcttta ccgtctataa cgacggaatt gcccactatg
     3721 gcagctcact gattacctat ctggtcagta tcggagttct ggtttttgga gtaaatttca
     3781 acctctacta ctacctcatg ctccgtcgca tcaaggcctt ctttggagat gaagagctta
     3841 gggcttactt ggtcattgta gtggtttcta caggcttgat tagcctcaac accctctacc
     3901 tctacccagg gttttcaaag agctttgaaa tgaccttctt ccaggtttcc aacatcatta
     3961 caacaactgg ttttggatat ggagatatta ccaactggcc cctcttctcc cagtttatcc
     4021 tccttttcct catggcaatc ggtggctctg ctggatcaac tgcaggtgga ctcaagatta
     4081 ttcgaggcct catcctttca aaaattgcca aaaaccaaat tttgtcaatc ctatcgcccc
     4141 accgtgtttt gaccctccat gttaataaaa cggtgattga caaagatacc cagcataaga
     4201 ttctcaagta ctttgtcatc tatgctatga ttttgctagc gcttatcttt attgtcagcc
     4261 tagatagcaa tgattttctg gtcgtgacca gcgctgtctt tagctgtttc aataatatcg
     4321 ggcctattct aggaaccact tctagtttct caatctttag tcccatctca aaaattctcc
     4381 tatcctttgc aatgattgca gggcgcttgg agatttaccc aatcctcctt ctctttatga
     4441 agagaacttg gtctaagaga taaaatacaa aatccctctt tttacaattg tgaaagaggg
     4501 attcttatat taaaaaaggg ggaacgtttt taaatccaag cctatctgta agcaaaatcc
     4561 ttactcaaat aaaagatgga aaaacttact acacagcttg gtaatgctcc aatacaaatt
     4621 gtaaggattt acttggactc tttgttaaat gaatatattc cattcctctg gtagcaacta
     4681 tctgaatacc taatctttct gcatccacct tcatctctct ataataactc cgaacttgtg
     4741 gagccagaat aattaaatca taaacaccca taatatcata atgagctccg tacgctcctg
     4801 aattcgcaat cactctaact tctgctaatt gagcgccctc gttaattgca ttggctaatt
     4861 gcgcacttgt tccagaccct gcacaaagaa ccagtacttt caactcctta ctttttattt
     4921 catctaactc accaggcatt atttcacttg tctcctcctc taaaataata tcatttgagc
     4981 ttgcaacatc ttctttcatc agtaactgtc tatcatacgc tctacagaat ggcaaataaa
     5041 tcaatatgtc gacaactaaa atcaaagata aaaatacaaa ggagatagat tgaaaattcg
     5101 ttccaattaa caatcccaag ggaccaggaa aggcccaagg taactggata taaaatccat
     5161 tcattccaaa gaaatcaata aagacctttc ctagaaatac attcactggt ggagtcatca
     5221 aaaaaggtac aaaaagatag ggattcaaaa taactggcat accaaataga agaggttcat
     5281 tcaccgcaaa taaaacagga gtaattgtag ctttacctac cgattttaat tgtttagacc
     5341 gcataaagaa aatcaaaata aatggtacaa caaaggtagc ccccgttcct ccgatagcag
     5401 cgatataatt tccgaaatta ggcgttaaag aatgataagg aaactgccca tttgcaagaa
     5461 gttgagcatt ctcttccata ttgctcagtt gtaaagctgt aacagcaggt aagacaatag
     5521 atggtccatg aattccaaca aaccaaagca aagcgcacat aaaccaaatc aacatcatag
     5581 caggatatga ttctgccccc ttaaaaatag gagtcaatag ttgttgaaat acttgggcaa
     5641 aaggaacatc taaactaaac cgagatacaa tatctaacaa accactaata agtaaaacaa
     5701 aagaaaaagg aaaaatatct ctaaaagctt gtgatatagc cccaggaact tccttaggta
     5761 aatgaatagt aatgtctcgc ttaatacaga atcggtaaac atttacagta ataaaggtac
     5821 tgacaaacga agttatcaat ccttgagatc ctaaatagtt tgtcgacaaa cttgtagatc
     5881 ccgttttctc atcaactaca agcgttacag ttaatacaag atagccacat atggccgcca
     5941 acattgcaga agtatcattt attacctttc caggaggcat ttttctatta acatttccag
     6001 ccaatgactt tgcaactgtc cctgaaacaa taacacccaa aactcccatg gttagcatat
     6061 acactttcct catccaggca atagctggac tcggtaactc tactccgaaa tttttaggaa
     6121 tcataataat aatcatcaaa aagcttgaaa acataattaa aggcatagta gcaataaatc
     6181 catctttaat agccatcata taattattag tagaaatttt ttcaaatttc ccttgttgct
     6241 tttcaatggc agcgactaac ttatccataa aaactcctcc tgtaaaaaat ttaagctcat
     6301 agttaaaata tattaaacat cagtcaaatc gctaacctta attccgtttt aatcctttta
     6361 cccaaagagc acttttctta atatatctct tctgtgtttc ataatcaaca aagaaaagac
     6421 catatcgctt attgtagcca ttcgcccaag aaaattgatc ttgcaaagac catataaaat
     6481 agccttgaat attgactccc ctatctcgag cctctaaaac tttgtgtaaa tgttgctcaa
     6541 tataacggat tcttttactg tcatcaataa tatcattctc gccctctggc ttaacctctt
     6601 taagggctgt accattttca gttaaataaa tgaccggatg ttgaggatat tcttctttga
     6661 tacgaagcaa catatcaaat aacccttgag gataaatatt ccaatcccaa tctgttgtcg
     6721 gaataccagg cttttttaca aattcaccta gagcatttaa tttaaaagaa gactgtcctt
     6781 ttattcctgt tgaattaaat tcttgtctat tttcaccttt gtattcacga ataaattctg
     6841 aacgataata attcatccca aacatcgtat taagaggtgc agctcttttc ataatttcta
     6901 aatcaccatc ttcaataata aagctagaat tattagcttc caatatttga ttcaagtgaa
     6961 aaagcgtgtc atcactgtag tagcccaaaa aagttccatc taatagaaat ttattattat
     7021 aaacatcata ccgtttagct gccaaaatat tttctttttg cccatcaata ggatagcctg
     7081 gctttaaagc atgaatacaa cctacctttc cctcaatccc taattgatga aattcgagga
     7141 ctgcaagagc atgcgccaac aacatgttat gatttgcttg aattgcttca gataattgaa
     7201 aatggtgatt cggaggaaac tgacctccta tgtattggcc tgcagcaaga gacatcagtt
     7261 cattgattgt aaaccaatgc ttgacttctg taaattcttg gaaacaaaag cgagcatatc
     7321 gtatgaaacg atcaatattc tctctattca accaatcccc tgtttctaac attttctgag
     7381 gcgaatcaaa atgatgtaaa gaaacaaacg gaatcacatt atattttaag caggactgaa
     7441 agactctatg gtaataacga acagccaata tattcggctc cccatctata tcaggaaata
     7501 tacgaaccca agaaatagat aaacgcaaag cctgcaaacc atgttctgcc gccaaagcta
     7561 tatcctcttc gtaacgataa taaaaatcac tagctggatc tggtaagaac ggactatttt
     7621 cttgcaaata aacatcccac atatttattc ctttgccatc taccctagtt gcaccctcta
     7681 cttgataagc agcggtagca gctcctaata aaaactcctt cggtaaggct ttaacctgta
     7741 aattttccat gactaatcct cattgcaaac caatatgttc ttcaatcttt cgaattcgtt
     7801 catattcatc aataaaaaac tttacctgat catacaatag catcgttgtc atcaaagtat
     7861 cttgaccatg aatcatgata aaactcactt caaaattatc tccagatgcc tcctccgcta
     7921 ataaattagt ctgttctcga tgggcagaca ctattgagtc gtttgcagac tcaaccaatt
     7981 ctcttgcctg ttcaaaatat ccatctctcg caaacgccaa agcatccatc aaatctgacc
     8041 ttgcatctcc tgcgtaagcg acaattgtaa aaccaagcat atttatttct tcttttgatt
     8101 tcatctttct tcctcctcaa tcttgaacca tcttttacac atcattatta aaatgacttc
     8161 gctacgatac aaatagaaag tcgaccctaa atatcttaaa aatccttaaa tttcataata
     8221 tttaggatca atcttctctg aataccttgg ttcattttct agacttatga tttataagtg
     8281 gaaaccagcg taattttttg ctacttcaat tacacgataa acatccaaat tagattggaa
     8341 tagcagttcg tgcccacgga tataattcaa cacatttctc cattcatctt gagcttgcct
     8401 tgtttttcca gttgcttgaa gatataaagc cttcgccatc ttaacaacat attcacgatg
     8461 ataactgagc tgtttccatt catccttttg actatttaat aacttagaaa tattttcctc
     8521 aataattggt aaaaaattat cagctagatt gtaagctata taataatgat tcgctaaaac
     8581 atcattttga cgactgccaa ttgcattaaa atagtcacaa gaggaataaa tggataattt
     8641 ttctaaatat tcaacaacag actgccaatt ttccccatac aaagcaaaaa agtattcttc
     8701 aatcaattct tcataacttc ttgtcttctt ccagagcatt tgccccatga cataattagg
     8761 aaaattatga gggaatccgg cacgtaattc ttgacacgaa atgtacccat tcaaatgtag
     8821 gtttgaaaga taagatacat ctctgtaaat agtttgacta attttcatat agcctaaatc
     8881 gccataatga gcacgcccta aaggatagtc atatacgaaa ctatctcctt taaatgtttt
     8941 ttgccactca aaaagataag ataaattttc ctcaagagaa ttcggaagta taattttatt
     9001 acgcatataa ggtttaggcg taggtatgga attgtcaaaa tctacatctg cataactcat
     9061 ttcaaatgtt cttgtaatcg gtgcaaacat catggtaaag cgttcaggat tatctaattt
     9121 ttctttctga ggcgcccata acaactcatg gtaaagcaga aaacaaatct ttgtatctaa
     9181 tccctcactc gttaaagccc tatccaattg attgagaata cgaatatact gatccgaaac
     9241 caattcttgt ctacagtttt cgcattcaca aatattatta cgagcatccg acaaccatac
     9301 atgtaagtag ttaacatcag gtcttttctt ggcataatcc ttgataattt ctaccatctt
     9361 atcagctaca tctggatttg aaaaatccag gctggttaaa atcggagccg tattaaacaa
     9421 ttctcgtttc ccgtttattt cagcgacata gggtttcttc tcctctgaaa tactaagacc
     9481 tgattcccag ccaaattttg aagagtaacc taaaacctca cctgtccatc catgaccaac
     9541 acgatgatga ataagacctc ttttttgcaa ttctttatcc aacctatcac tcaattcttg
     9601 tactaattca tttgagaatt tttctttatt taggtatgga ttaaactcat gttcatacca
     9661 acgtttcaaa aaagagtaag gattttcaaa ctggatgaaa aaactgttca tcccaatctt
     9721 aggtagccaa tcaatgaaat ctagtatatt ttcaaatgaa tccgctccct ctatacatac
     9781 tcccctatga tagtaactgg ctgtttcatc tatagatagc tgtttatcta aaaaatcttc
     9841 aaatcgtaac tctggaacaa agtcatgtct gcgccctggt ctagtataca caaccccaaa
     9901 ttcatgaaac attcggtaaa ttccaataag taaacctaca attgtattag actcaattct
     9961 ccccttaccc ttgtctaatg agataagaac atggtctttt ctgtcctctt tattgactaa
    10021 tcctacaaaa atatccgctt ccttctcctc gacaagctct atgttagtct taaagacccg
    10081 attcatatag taacttaatt cctctaaggc aaagctagaa taagtatttt tctgttgatc
    10141 aaatactctg ataaccatct tatttccctc caacagtagc tttttctttt tcaagattgc
    10201 gtttatctgc aattttgaaa aatggataat aaattgctac agaaaccaaa atcaaaacaa
    10261 cttgtaatag agctcctcgc caactagctc ctgtcgcaag gaagcctcct atgatcggtg
    10321 gcattgtcca cggaaggatt acacctgttg tatagggtac taatcctaca gccattgata
    10381 cataggtaat caaggcattg attgtaggag tagcaataaa cggaatcagc ataatcggat
    10441 ttaaaactgt tggaaacgta aatagaatag ctgtattgat attaaaaata gacggtataa
    10501 tagctagctt acctaatgtt ttattcgcac gactcttact aaataggaag agacaaatcg
    10561 caagaccaat agtcgctcca ccgccaccaa taaatacaaa taaatcttta aacggtaatg
    10621 taataatgtg ttggagtgtt tgtcctgcag ccacagcttc ttggttttct gtagtaaatt
    10681 gtaaccaaac tgggtctaca aaagcattta aaacttgtcc cccattaact ccacagaacc
    10741 aaaagaatga gtttacaata acacatagaa tcatacctgg aagcgttcct gcaattaact
    10801 taagcggtgt tccaacaatt gcacctagga gtccgttgag acctcctgca actcctgccg
    10861 cttcaagacc ttttaagacc agagcccaca aaacaaaagt agtaaagcca ggtaaaagag
    10921 ctgaaaatga tttacttacc acatctggga caccacttgg catttttatc gtaattccgc
    10981 gctggataaa catacgataa atctctgctg taaccaatcc aatagtaatc gccataaaca
    11041 aagaagatgc attcaggcta gaaaatggta ttgcgccgcc aaataactgc ttgacctgct
    11101 ccccattttt atcataaacc atactcgaaa aacgaggtgc catcaataca aaactggata
    11161 aggctatgat ccctgccgac ggaccatctg taccatatcc ttccgacaac ctgtaggcaa
    11221 taccgaaaca agcgaccaag gagataatcg ctactgttga ctgattcata gttgtcaaga
    11281 tacttagcaa tccaacacta ttcagccaat ttgtaaaagc ctcttgaggc caactgatta
    11341 gaataagaaa taaagagccg actaaaatca aaggcattgc catcataaat ccttcacgaa
    11401 tagctttcaa atgccgttgg ttgccaactt tttcaccaaa cggagcgact tttgtttcta
    11461 aaaacgctaa aatattgttc atcaccggaa ctcatttttt tattttttat aaattaaata
    11521 taccactaac agaaagcgtt gtcaatatat tttttgttgt ttttacaata tttttttctt
    11581 tgtatagaat aataatttta attgattttt tcttatgttt aatataaaat aaaccaaagg
    11641 aggaaacaaa tgattacaaa acaatcagcc tttttacaaa atagagccac tatactggag
    11701 tttctatatc gaaatccagc aacatcccgt acagatattg ttaatgaaac aggacttact
    11761 ccagcaacaa cgactaacat cataaaagag ctaagcgaac agtccctcat atacgaaact
    11821 ggtgatgaat ttagcgagtt ttccggatct ggaagacgta gaaaaactat ttctatcaca
    11881 gataatattc cgtatgttgt tggaggtatt gaaataaacg tcctaggtat ctttctcagt
    11941 ctatgtgact tacaagggaa aactcttttc gagacagaaa ttttgaatga agattatcct
    12001 atttcagaaa tcaattccac cattaccaat atgataaaaa cagctataga gtacgtccct
    12061 ttggaaacaa aattacttgg atttggctta tcaatacctg gacattataa caaagactcc
    12121 ggaagtatca ttacaaacaa ccccatatgg gaatctttta atttattaaa tgtaattaaa
    12181 agattcaatt ttccttttat tgtaaaaaat aatatcgatt gtatggctat aggacaatac
    12241 ctttttaatc cacacaatac ccccgataac tttattttcc tacacgctgg attaggtatt
    12301 tacacttcct ttttcacaaa agaaaaaata ggagcctcta aaaatcctta tatcggagaa
    12361 attggacaca ccattgtcga attgaatggg caatattgtg aatgcggaaa aaaaggttgt
    12421 ttacaaacat atatttcgga tgcttggtta atcaaacatg cccaattatt atttaaaaat
    12481 tctcaactaa ctgtactaaa aagccttgta aagactgaaa aagacattca tttagatacc
    12541 cttttaacag cttacaactt aggcgactct gctttacgcc aacaaattga taaaggagtc
    12601 aatttattgg ccacttctat tgcaaatctt ctcctcatca atcctgctga taaaatctat
    12661 atcaacagtc aattgcttaa ttatcaacct ttcactcatg aagtcaggga taaaatccaa
    12721 gatcagctcc acttcgttcc ctttactcgt aatatagaaa ttgaaatttt accttacaac
    12781 aaacatcgtg gaagtatagg agcttgtgca ttagctatcg ttgctttttt catagagcat
    12841 agcaatgtat tacaagatat tatttcacct taatatatat tagaaatcta tagacctgtt
    12901 taaatcaact ataacctgta gtaaatatct cgtatttaga caatatgaaa acaagacgac
    12961 ttccatatag gaaaccgcct tctcgctatg ctgcatgaaa ctgtaaaaat agatgaaatt
    13021 gatagcggta gtcagatcaa gaaacatcgt aactctaaaa agtcacaata tataagttca
    13081 tcctcggaaa aatatcattc taattattga aatgcctaca tgaaaagaaa cgttaaatgc
    13141 tcataaaaca acgaatacag gtatcaaaac tatgacaaaa caaatcccta aatttactaa
    13201 agacactgct caactttaca cctgtaaatg gttgttgtat aataaagtta caaagatgta
    13261 cgaccacact attgtaaatc atagtgttcg cgaatatatt actgatagca tttctacaaa
    13321 tacaattgaa gaggctgaat aggctttaaa cgaaatatgt cagtgttttg gcattcctag
    13381 ccttcatatc atttaaagaa ttgtatagat aaaatttttt ccaatacaga cactcgtaac
    13441 aactgcttca tttttctacc aacatattta ggaacaggat aagatacaag aggattaatc
    13501 catagctcag ttctatacca atctaagaca aataagctaa aaaaacgatt gataataagc
    13561 aaatagattc caaattttct ctatctgctc attttaataa acaatactag tgtaactatc
    13621 cttccagtca gaagcttgtc aaatcacacc gaaaattctt ctaaaattta tctcgttagg
    13681 caatcaagca aaaactcgac gatagtacaa acattatcat acagaattga cttcctaaat
    13741 tatatacttt agtaaggttt tcggataaga aaaaaggttc attttacatt tctaaacatt
    13801 cttttctaag atgaaaaaca gaatttttcg attgtaattt aaagcaacaa gaagattttc
    13861 agtatcatcc tatagatacg agctaattaa gaaaaactac atttttgaat ataaactaca
    13921 ataatataaa ctaaattcca taggaggaag acaatggatt ggtacgatta tatgatacag
    13981 gcatccaaac aatcacaatt caacgcaagc cattggtttc gctatttgcg aaaagttatt
    14041 tttgaagact attcttattt aacaaaccaa gatgtagaaa agttgctaga ctccaaagaa
    14101 ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc aagagcatac tccaactcat
    14161 aaatatgtga tttcattaaa taaacctgct aagttaacca atgttcaaaa attgatggag
    14221 aaatacaaac atggataaaa tgaaaccggt cttccaagcc ctaaataaga aattaattca
    14281 ggaaaatctg actttaacaa ttatggttat gtcttagaat atcatggttt acgtgccaca
    14341 caagatgttg atgcttttta tgaccaaaat cagaaaatca atgaaattat tgctcgtgta
    14401 ggaaaacagt ttaatcttaa catccacgaa gagttatggc tcaacaatca tgttgcaaag
    14461 caaatatgaa caaacaacct cccttaagtt tatgcgaatc cttgtactca tttgagaatc
    14521 tgaccgtttt agtagtccct attgagtatg tgttaggaat gaagatgatg agtatcagag
    14581 agcaggattt gaaagatatt ggtgcaatta ttaaatacaa aaactttcat tcaccattcg
    14641 atacctttaa atatttaaaa gacatgggat ttgatactat tgacctctct gttctattag
    14701 aaggttttag ttatgcttat ggtatggatt ggttagaaaa attttttaaa gaaaatcaag
    14761 ataaacttcg cgaattttat taacagtatc aaacaagatg attgtcaatt agccagtcgt
    14821 cttgttttta tgatggaatg aaataaaatc ggaacaaatc acttagagaa ttccagacaa
    14881 tttctagcac tgtttgagac gtttcagcgg actatagcaa aatgaaataa gaataagaca
    14941 aatcgatcag gacagtcaaa tcgatttcta agaatgtttt agaagtaaag gtgtactatt
    15001 ctaatttcaa tctactatat ttcagattcg gtccactatt ccttcccaaa aac
//
LOCUS       DAVBCR010000051        14733 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCR010000051 DAVBCR010000000
VERSION     DAVBCR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.051"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            244..1110
                     /locus_tag="VQ508_001451"
     CDS             244..1110
                     /locus_tag="VQ508_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490428.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            1447..2004
                     /locus_tag="VQ508_001452"
     CDS             1447..2004
                     /locus_tag="VQ508_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2490429.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            2225..3331
                     /locus_tag="VQ508_001453"
     CDS             2225..3331
                     /locus_tag="VQ508_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2490430.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            3307..4698
                     /locus_tag="VQ508_001454"
     CDS             3307..4698
                     /locus_tag="VQ508_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490431.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            4695..5873
                     /locus_tag="VQ508_001455"
     CDS             4695..5873
                     /locus_tag="VQ508_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490432.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            6298..6531
                     /locus_tag="VQ508_001456"
     CDS             6298..6531
                     /locus_tag="VQ508_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2490433.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            6814..8763
                     /gene="mutL"
                     /locus_tag="VQ508_001457"
     CDS             6814..8763
                     /gene="mutL"
                     /locus_tag="VQ508_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2490434.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(9088..9555)
                     /locus_tag="VQ508_001458"
     CDS             complement(9088..9555)
                     /locus_tag="VQ508_001458"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2490435.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(9556..10761)
                     /locus_tag="VQ508_001459"
     CDS             complement(9556..10761)
                     /locus_tag="VQ508_001459"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2490436.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ508_001460"
     CDS             complement(10781..11416)
                     /gene="ribE"
                     /locus_tag="VQ508_001460"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2490437.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ508_001461"
     CDS             complement(11401..12501)
                     /gene="ribD"
                     /locus_tag="VQ508_001461"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2490438.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(12636..12750)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ508_001462"
     CDS             12907..13500
                     /gene="ruvA"
                     /locus_tag="VQ508_001462"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2490439.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            13510..14073
                     /locus_tag="VQ508_001463"
     CDS             13510..14073
                     /locus_tag="VQ508_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2490440.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            14070..>14733
                     /locus_tag="VQ508_001464"
     CDS             14070..>14733
                     /locus_tag="VQ508_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490441.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAI"
BASE COUNT         4636 a         2397 c         2761 g         4939 t
ORIGIN      
        1 gaaaggggct gaaagaacta ggggaacagt tgtaattaga aggtgatagg tatgaaaagt
       61 ggcgttgaag ctggataagt ttataaagag tttgaatgaa gaaatgaaaa gtaaataagt
      121 tgatgatgaa ttgcaaaaat agattttata tacaatttgt atctttcaaa tcattgattt
      181 gaattttaga tttaatcatg ttacaattaa aaatgaaatc aaatagaaga ggaagatgca
      241 aacgtgggca cactattagc aacaagatta aaaaatagac gaaaagaatt aaaaatgtct
      301 cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt agagagcgga
      361 gagtttactc ctggagcaga tttcttgcat gcccttgcta agaagttaaa agtcagtatg
      421 gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga atttaagaaa
      481 ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat atatgaattg
      541 gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga atggataaaa
      601 tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg attagagggg
      661 gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa caccctattc
      721 aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga aaaactagag
      781 tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc tattaagttt
      841 aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc tattactaaa
      901 attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt ggctgatttg
      961 tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt gaaagaatat
     1021 tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac tctgaaagtt
     1081 gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga gcatcgtttt
     1141 tcaaacctag ttcagataca aaaaagtatc aaataaaaaa aaataaaaaa acattataaa
     1201 aacaattgac aagctaattt tgaagtgata aaataaatgc aagaaaacaa aaggagaggt
     1261 atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta attctgttag
     1321 gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct ggtcctgcga
     1381 attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa tgggtaaaaa
     1441 gattgtatgg ataagaaaaa tttacttgta ttggttactg gttcggtagg tgccagtaat
     1501 gtagatacat acttatatta tataaaaaaa ttctataatg ttaaagtgat actttctgaa
     1561 aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa agtttatgat
     1621 gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc tgacttactt
     1681 cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat tgctgatgat
     1741 ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc caatatgaac
     1801 tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa ggagttggga
     1861 catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa agcaatagat
     1921 tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa attagaaaat
     1981 attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg aaaaaatgga
     2041 aaattcatca atcgatgtag atatgctgtt ggaagaattg acacaagaag caatggtcgt
     2101 tgttgctgtt gataaggact gttaatttaa acttatggca atatatgaaa ggttactgga
     2161 tgttttaaat tatgcaggca gtagcctttt attatataca aatggataaa gtaaggataa
     2221 tacaatgatt aataaaaaaa tacaacaagt tgttttggaa tcattacaga attttttgaa
     2281 tgggaacttc atttcgcctt gtgtagtcta tgattttggc ttgctggaaa ctgtacttga
     2341 tgaatttaaa aatcaaattc ctgtaacatt caattaccaa cttttttatg ccgttaaagc
     2401 aaattcaaat gagaagatac ttgaattctt agtagataaa attgatggag ttgatgtggc
     2461 gtcattatct gaattagatg tggctaaaaa atttttccca ccaactcaaa tttctgttaa
     2521 tggtcccgca ttttcttatg aaactttata taatctgatt aaaaaacaat ataaagttga
     2581 tattaacttt ttggaacatc ttcaacaatt ttccccaaaa gaatctgttg gaataagagt
     2641 aacggagcca gatgaactta ataatcgtat gagtcgattt ggaataaata tttgcagtga
     2701 taattggact agtaatttac aaaatccttt aattacacga ctgcattttc attttggaga
     2761 aaaagatgat aaatttattg ttaagttaga taaaatatta tttaagttac aagaaattaa
     2821 taaacttaga gaggttagag aaataaatct tggaggcggt tttatgaaat tatttatgga
     2881 aaatcgtttg aaagaatttt ttctatcact tatggaaatc tataaaaagt acgatattga
     2941 tagtactgtg actacaataa tagaaccagg tagtgcaatt acttcatttt ctgcctatat
     3001 gattactagc ccagttaatg ttagtgaggt gaatgagcag caggttatca cgttagacac
     3061 atcaatatac accaatacat tatggtttgt tccgcatatt attacaacgt taaattcaag
     3121 tagtaaagag cgttatagta ctattctcta tggtaatacc tgttatgaac atgacaagta
     3181 taaaatgaaa gtttcgcttc caaggttaac tcaaaatagc agtatagtgt tttttcctgt
     3241 aggagcttat ataaaaagca atcattcaaa tttacatcgt aatgatttta tgcgggaggt
     3301 atatttgtgg acaaaaaact tgacatatta gataaagtta aggaatattt aggaaataaa
     3361 actactcaaa ttctggataa tcaatataaa gaatttttga aacttaatga tataaggcga
     3421 gcgtttggta tttcagaaaa agtattaaac aattctttta attttacgag taaagaattt
     3481 aatgatttaa ttaataacga aaattattta ttcgaatatg catgtagaat tagagaggaa
     3541 tggagaaaaa aatgctttaa tcattcttat cgttttctat gctcacctat aattacagat
     3601 gattttctta acacgaagac attgagaagt agccaaattg aatataaata tgagcgatat
     3661 ttatcgaaaa gttcgatagg cgatagagcg gttgatggct ttgtttcctt caatacttta
     3721 acagctaatg gtatgtctgc tattaaacta tgtcttgaga tattaaactc tattttcttc
     3781 aagaagaaga ttgatttatt atattcaacc ggatattatg aaacaagatt tttattaaat
     3841 aatcttgcta aatcaggtat tagttgctat gaggtaagta attgtgaatt ggataaagat
     3901 aaattttata atgtattcat gatggaaccc aatcgagccg atttaacatt acaaaaaact
     3961 gatttcaaga tagtagaata ttttgttaag tataaaaata attcaataaa agtcgttatt
     4021 ttagatattt catatcaagg ttctaatttt aaattagtag aatttttaga gaaatttaaa
     4081 tttgcgaatg taattatttt tgtggtacga tctttgataa aattagatca aatgggatta
     4141 gaattgacaa atgggggaat aatagaagtg tttattccta atcatttgag aaagttgaaa
     4201 aattttattg aagaggaatt caataaattt agaaattctc acggagctaa tctaagcctc
     4261 tatgaatact gtttgcttga taattcttta accttaaaaa atgattggaa ctattctgat
     4321 ttagttatga aatttacgag taatttttat gctgatataa aagacttgtt catggaaaat
     4381 tctgatattg aaatcatcca tgaagaggga gtaccttttg tatttttaga tttaataggt
     4441 gaaggtaaaa aagaatatga aatgtttttt caatggttaa acttctttta caaacagctt
     4501 ggaatcacat tgtatgctag aaatagtttt gggtttcgga atctaacagt agagtatttt
     4561 ggaattattg ggacagaaag atatatattt aagatttgtc caggtgttta taaagggtta
     4621 agttattatt tgatgaaatt tttattaaaa tctttttcaa atgaatattt aaaaactact
     4681 gatgaggtta atagatgaaa aatttgataa agttgctaat aattagattg attgttaact
     4741 tagcagacag tgtattttat atagtagcat tgtggcacgt tagcaataat tattcttcga
     4801 gcatgttctt aggaatattt attgcagtaa attatctacc ggatttgtta ctaatctttt
     4861 ttggaccagt tattgacaga gtaaatccgc aaaaaattct tataatatca attttggttc
     4921 aattagcagt ggctgtaata tttttattat tattaaacca aatatcattt tgggtgataa
     4981 tgagtctagt gtttatttca gtaatggcta gctccataag ttacgtgata gaagatgtgt
     5041 tgattcctca agtggtagaa tatgataaga ttgtatttgc aaattctctt tttagtattt
     5101 cgtataaagt attagattct atttttaatt cattcgcatc atttttacag gtggcagtag
     5161 gatttatttt attggttaag atagatatag gcatattttt acttgctcta tttatattgt
     5221 tgttgttaaa atttagaact agcaatgcga atatagaaaa cttctctttc aaatattaca
     5281 agagagaagt gttgcaaggt acaaagttta ttttaaataa taaattatta tttaaaacca
     5341 gtatttcttt aacgcttata aacttttttt attcatttca gacagtagtt gtaccgattt
     5401 tttctattcg atattttgat ggtccgattt tttatggtat ttttttaact attgctggtt
     5461 tgggtggtat attgggaaat atgctagcgc caatcgtaat aaaatattta aaatcgaatc
     5521 aaattgttgg tgtatttctt tttttgaacg gctcaagttg gttagtagca attgttataa
     5581 aagactatac tttatcactt attttatttt tcgtttgttt tatgtctaaa ggagtcttca
     5641 atattatttt taattcgttg taccaacaaa tacctccaca tcaacttctt ggtagggtaa
     5701 atactaccat tgattctatt atttcttttg gaatgccaat tggtagttta gttgcaggaa
     5761 cgcttattga tttgaatatt gaattagtgt taattgctat tagcatacct tattttttgt
     5821 tttcttatat tttttatacg gataatggat tgaaagaatt tagtatatat tagaaatgtt
     5881 tatgttcatt caaaagcata atgactataa ctgaaaaaga aaagtgatat ctttaaggtt
     5941 gttcttcttg gtggtgagat tcgtgagaca acccaagctt ttgtcggaaa gattaccaat
     6001 gctttgatgg ataggatgta ctttagcaag atgtttttag tggtaacggt atcgtggatg
     6061 gacgtgtaat aacctcttct ttcgaggagt attttactaa aaaactagcc ttggagcgtt
     6121 ccccagaaac ggacttactc attgactctt caaagatttg gggagaagat tttgcttcat
     6181 ctgttccttg aaaaaagtca cagcagtcat cacagacgat agtactgaac aaaactatga
     6241 agagttagaa atttatacgc aggtgattgt ataaaggatc tggaaataga taagaagttg
     6301 attagtattg acctaggtgg tacaaatatt aagattactg ttctttcaaa tgacggtgag
     6361 attgaaactt tgtggagtat tacaacagat acaagtgaga aaggttctca aattatatcg
     6421 gacatcatca gttctattaa aaataaattg accgaacgga atattcctga tagcgacctt
     6481 cttggaatcg gtatgggaag ttgctcatca tactttcctt gtaaatcata ggggctataa
     6541 actctccgtc tacttgtcct gcaacaattg aagtctgctc aaaacgccgt ccgctaatct
     6601 tttcatagac tttctccctt ttaggagcct agctttctag tttgttcttt gatttttatt
     6661 gagtatacca ctattttact ccctctggca agggactttg tctatgtgga gggattgggc
     6721 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaatcaatt
     6781 tctaggaaaa tagatacgga aaggggctga aagatgtctc atattattga attgccagag
     6841 atgctggcaa accaaatcgc agctggagag gtcattgaac gtcctgccag cgtggtcaaa
     6901 gaattggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
     6961 gctggtctca agaaaatcca aatcacagat aacggttatg gaattgccca cgatgaggtg
     7021 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
     7081 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
     7141 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
     7201 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
     7261 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
     7321 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
     7381 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc
     7441 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
     7501 gatttcgaaa tttcaggttt tgtgtccttg cctgagttga ctcgggctaa ccgcaattat
     7561 atcagcctct tcatcaatgg tcgttatatc aagaacttcc tgctcaatcg tgctattttg
     7621 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
     7681 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
     7741 aaggaaaaag aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa
     7801 accttgattc cagatgcctt ggaaaatctt gccaaatcga ctgtgcgcaa tcgtgagaag
     7861 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
     7921 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
     7981 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
     8041 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
     8101 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
     8161 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
     8221 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
     8281 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
     8341 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
     8401 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
     8461 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
     8521 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
     8581 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
     8641 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
     8701 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
     8761 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt
     8821 gttcaaaaac cttgatttta tgcgttttat catggaaata gttacttcat gttttcctaa
     8881 ttcttttcga aactcttttt aaaccacgtc agttttatca gtaatctcaa aacagtgttt
     8941 tgagctaatt ttgccagttt tatctgtaac atcgaagttg tgtttgacca ctctgcgact
     9001 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
     9061 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
     9121 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttggtac cagctcgttc
     9181 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
     9241 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
     9301 attccctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
     9361 ttttgatgca atcagtggta tttcaaaagc tcctggaacc caggctacct ctatatcttt
     9421 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
     9481 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
     9541 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
     9601 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
     9661 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
     9721 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
     9781 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
     9841 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
     9901 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
     9961 gcaacgtaaa gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac
    10021 attggcttca tcctctatat ttcccataat aagagcaaga tgatgttccc catttagttt
    10081 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    10141 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    10201 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
    10261 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    10321 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
    10381 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    10441 tgcggtcagt cctctctcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    10501 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
    10561 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    10621 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    10681 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
    10741 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    10801 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
    10861 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
    10921 gttccaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    10981 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    11041 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    11101 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
    11161 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    11221 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    11281 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
    11341 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    11401 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    11461 agaaaaagca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    11521 gacaggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    11581 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    11641 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    11701 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
    11761 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    11821 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    11881 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
    11941 aacatggtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    12001 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    12061 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    12121 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    12181 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    12241 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    12301 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    12361 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    12421 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    12481 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
    12541 tactcaaggc atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt
    12601 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
    12661 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
    12721 agggaattac accctgccct gaagatagtt attcaattac agatgattat agtacttaat
    12781 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
    12841 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
    12901 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
    12961 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
    13021 caggttaatc aggaggctca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
    13081 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
    13141 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
    13201 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
    13261 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatggcctt
    13321 cctgccaagg tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa
    13381 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
    13441 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
    13501 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccact ctacatcgcc
    13561 tatcatgatg aggagtgggg ccagcccctc catgatgacc aagcgttgtt tgagttattg
    13621 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
    13681 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgacactgaa
    13741 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagat ttttgctaca
    13801 cgcgctaacg cccaagcttt tctacagtta caggcagagt acggctcttt tgatgcctat
    13861 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
    13921 ccagctaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg agacttcaag
    13981 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
    14041 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag ggaattctga
    14101 tttttgcgat tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt
    14161 taatgccatc tgccattgca aattatcttg tttatgtagt tttagctcta tatggctcct
    14221 tcttattcaa ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat
    14281 tcttctttgg cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
    14341 ttgtatcaga gatgttgaag cagtttgtgg ggctagtcgg acaaggtcta aatcagtcta
    14401 atattcaaag cacttttcaa gagcaaccac tactgatagc tgtttttgct tgtgtcattg
    14461 gacctctggt agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
    14521 tgccaggttt actaagcatt attctggtag gacttgtttt tgctctgact catatgcaca
    14581 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
    14641 ctattattta tgtgaaagaa aaagagaata tctactatcc cctacttgtt cacatgttaa
    14701 gcaacagcct ctccttaatc attttagcta tca
//
LOCUS       DAVBCR010000052        14423 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCR010000052 DAVBCR010000000
VERSION     DAVBCR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.052"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            81..878
                     /locus_tag="VQ508_001465"
     CDS             81..878
                     /locus_tag="VQ508_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2490442.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VQ508_001466"
     CDS             1026..1148
                     /locus_tag="VQ508_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2490443.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            1170..2210
                     /locus_tag="VQ508_001467"
     CDS             1170..2210
                     /locus_tag="VQ508_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2490444.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2381..3655
                     /locus_tag="VQ508_001468"
     CDS             2381..3655
                     /locus_tag="VQ508_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2490445.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VQ508_001469"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VQ508_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2490446.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4811..5086
                     /locus_tag="VQ508_001470"
     CDS             4811..5086
                     /locus_tag="VQ508_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2490447.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VQ508_001471"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VQ508_001471"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2490448.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VQ508_001472"
     CDS             5960..7300
                     /locus_tag="VQ508_001472"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2490449.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VQ508_001473"
     CDS             7278..7490
                     /locus_tag="VQ508_001473"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2490450.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VQ508_001474"
     CDS             7487..8362
                     /locus_tag="VQ508_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2490451.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VQ508_001475"
     CDS             8355..9170
                     /locus_tag="VQ508_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2490452.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VQ508_001476"
     CDS             9163..9594
                     /locus_tag="VQ508_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2490453.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VQ508_001477"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VQ508_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2490454.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VQ508_001478"
     CDS             11270..11998
                     /locus_tag="VQ508_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2490455.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..13900
                     /gene="lepA"
                     /locus_tag="VQ508_001479"
     CDS             12077..13900
                     /gene="lepA"
                     /locus_tag="VQ508_001479"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2490456.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14059..14139)
                     /locus_tag="VQ508_001480"
                     /pseudo
     CDS             complement(14059..14139)
                     /locus_tag="VQ508_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         4292 a         2618 c         3418 g         4095 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt atttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaatacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ttatacttgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaagatgt cggttcaatc tttaccttcc
     2041 atgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga gagtcagtat gccgaactct tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc
     2521 tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2641 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2701 tcagttggct accttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2821 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2881 cgagttttat aaggatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcagacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcattctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttacc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa ccccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg tagatactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa cttaaaattc tttcagataa atatttagag tccatgacag
     4741 gttctggtgg gaacttaggt ctaggatttg gtgtgataat tccatgatgt gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5521 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggaa
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccataa aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaaatatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6361 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatcctacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aatccttgca ggttaccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg tctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 ttcgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcacag tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attgccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgaactatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gatctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagcc atgaatgaca tggaaagtgt cgaagagtat
    10381 gaccctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg
    10501 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10801 ggaaaattca gtcgtgaggg aaataaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagcaag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc tcaaaacctg
    11341 ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtccgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tagtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat
    11701 tgatttgacc ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggagct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12301 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12361 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12421 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12481 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg tgcgtacaga
    12541 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12601 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12661 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12721 tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12781 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg
    12841 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12901 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12961 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    13021 gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13081 tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc ttggacttct
    13141 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgacc tcatcatgac
    13201 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13261 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13321 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13381 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13441 tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13501 tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat
    13561 tcttctcaat ggagataagg tggatgccct cagctttatc gttcacaagg actttgccta
    13621 cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga
    13681 agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13741 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13801 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13861 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13921 ttgttcacag gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt
    13981 ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt
    14041 atggttactg acttcgtcag tttcatctac aacctcaaaa ctgtgttttg aacaacctgc
    14101 ggctagcttc ctagtttgct ctttgatttt cattgagtat tagaacagaa aaaatgcttg
    14161 gagtatttgt ttgtgtgttt acttttatat aacaaactat aaacacaaca aaaatataaa
    14221 aaaagagaca aaaaagaaca gaaagtaatt aacaacgatt tcatatagtg ttatacttat
    14281 agcataaagt taatggaaag aagggaaaac cttatggtat tagattattt ctttgacaaa
    14341 aaccttgtgt tttgcttaga agcggataat caagaataac tctttgatca ggtagcaagc
    14401 ttgttggagg aacgagaaat tgt
//
LOCUS       DAVBCR010000053        14384 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCR010000053 DAVBCR010000000
VERSION     DAVBCR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.053"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(54..1343)
                     /locus_tag="VQ508_001481"
     CDS             complement(54..1343)
                     /locus_tag="VQ508_001481"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2490457.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQTLRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1421..2089)
                     /locus_tag="VQ508_001482"
     CDS             complement(1421..2089)
                     /locus_tag="VQ508_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490458.1"
                     /translation="MSLSMSSADGENLKNEKLSIKDQFLTIYRNLRLVFLKSGQKNFG
                     FMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQFCVLVAVIISSLTGND
                     YFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYVSGKVQPKISAMLMKN
                     LAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLTWMLFVGLSLLAIFLTILNL
                     KNDI"
     gene            complement(2401..2667)
                     /locus_tag="VQ508_001483"
     CDS             complement(2401..2667)
                     /locus_tag="VQ508_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490459.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFF"
     gene            complement(2857..3708)
                     /gene="rgg"
                     /locus_tag="VQ508_001484"
     CDS             complement(2857..3708)
                     /gene="rgg"
                     /locus_tag="VQ508_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2490460.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSYNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEKFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3832..4173
                     /locus_tag="VQ508_001485"
     CDS             3832..4173
                     /locus_tag="VQ508_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2490461.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4382..6085)
                     /gene="ilvD"
                     /locus_tag="VQ508_001486"
     CDS             complement(4382..6085)
                     /gene="ilvD"
                     /locus_tag="VQ508_001486"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2490462.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6318..7250)
                     /locus_tag="VQ508_001487"
     CDS             complement(6318..7250)
                     /locus_tag="VQ508_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2490463.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7247..8104)
                     /locus_tag="VQ508_001488"
     CDS             complement(7247..8104)
                     /locus_tag="VQ508_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2490464.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(8108..9454)
                     /locus_tag="VQ508_001489"
     CDS             complement(8108..9454)
                     /locus_tag="VQ508_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2490465.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9467..9751)
                     /locus_tag="VQ508_001490"
     CDS             complement(9467..9751)
                     /locus_tag="VQ508_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2490466.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9755..11785)
                     /locus_tag="VQ508_001491"
     CDS             complement(9755..11785)
                     /locus_tag="VQ508_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2490467.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            11977..12984
                     /locus_tag="VQ508_001492"
     CDS             11977..12984
                     /locus_tag="VQ508_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2490468.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            12987..13187
                     /locus_tag="VQ508_001493"
     CDS             12987..13187
                     /locus_tag="VQ508_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2490469.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13339..13521
                     /gene="rpmF"
                     /locus_tag="VQ508_001494"
     CDS             13339..13521
                     /gene="rpmF"
                     /locus_tag="VQ508_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2490470.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13537..13686
                     /gene="rpmG"
                     /locus_tag="VQ508_001495"
     CDS             13537..13686
                     /gene="rpmG"
                     /locus_tag="VQ508_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2490471.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14178..>14384)
                     /locus_tag="VQ508_001496"
     CDS             complement(14178..>14384)
                     /locus_tag="VQ508_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2490472.1"
                     /translation="YLNESGSMATGWVKDKGLWYYLNESGSMATGWVTVSGKWYYTYN
                     SGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         4625 a         3048 c         2476 g         4235 t
ORIGIN      
        1 aaaacagtgt tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga
       61 gtataaaatc ctagtttttc aaagatttct gagaagtttt ggctgattgt ctcaagtgac
      121 acttgcactt cttctcgggt ttggttgttc ttgaccgtca cttgtccgct ttcgacttcg
      181 ctctctccta gggtgatgag ggtcttagcc gcaaagacat ctgctgactt gaactgagct
      241 ttgagcttac ggttgaggta atcacgctct gctttgaaac cttgttggcg aagagtttgg
      301 actagttcta gggctttgac atttgctcca tcacccaaga ctgcgatata gacatctagg
      361 gcgttttcga tagggagggc cacgccttgt ttttcaagga tgagaagcag gcgctctaca
      421 ccaagcccaa aaccaaatcc agcagtttca gggcctccaa agtaagcaac caaaccatcg
      481 tagcgaccac ccgcacagac tgtcaagtca ttgccctcaa tctctgtgat aaactcgaaa
      541 atggtgtggt tgtagtagtc cagaccacgc accatattgg tatcgatgat gtaatctact
      601 ccaagatttt ccaacatctg acgcacagca tcaaaatgag cttggctttc ttcatcaaga
      661 aagtccaaga tagacggcgc attctctact gccaccttgt cttctttttc cttagagtcc
      721 aagacacgaa gaggattttc ctccaagcga cgttggctat ccttagacaa ggtctccttg
      781 agcggtgtca aatagtcaat caaggcttgg cggtaggctg cacggctctc aggatttcca
      841 agagtgttga ggtgcaattt gacaccttga ataccgattt ccttcaaaaa atgggctgcc
      901 ataacgattg tttccacatc ggtagctgga ttgctagagc caaaacactc aacaccaatc
      961 tggtggaatt ggcgcaagcg ccctgcctgt ggacgctcat aacggaacat aggtcccatg
     1021 tagtagaact tgcttggctt ttgcacttct ggggcgaaga gtttattttc cacataggaa
     1081 cggacaacgg gcgcagttcc ttctggacgg agggtaatat gacggtcacc cttgtcataa
     1141 aaatcgtaca tttccttggt tacgatatcc gttgtatctc cgacagagcg actgataacc
     1201 tcgtaatgct caaaaatagg cgtgcgcact tctgcgtagt tgtagcgttt gaaaatttca
     1261 cgggcaaagc cctcaacgta ctgccacttg gcagattcag caggtaaaat atcctgcgtt
     1321 ccttttggtt tttgtaattt catagggaat cctctttaaa cttaatagtc ttattttacc
     1381 ataaatagag ggattaaaac agtgagaaaa aaattaggat ttagatatca tttttgagat
     1441 taagaattgt taaaaaaata gctagcaagg aaagaccaac aaatagcatc caagtcaact
     1501 gcatgttcca tacggctact agtgaaaaac aagctgttcc cacaggtatg gataaggtaa
     1561 acaatagacc taaaaaatta ctagtacgag ctagaacctc tggagctaga tttttcatga
     1621 gcatggcact aatctttggt tgaactttac cagacacata cagagtaaag aagagaaata
     1681 gcaaaccaag cacgacttga ttgaataaat tagccaaacc aactagacta agtcctacgg
     1741 tcgcccacat catcaatcta ggcaaggact gcttcccaaa ataatcattg cccgtaaggc
     1801 tactgatgat gactgctact aaaacacaga attgattgat aaatagtgcc tctgtataag
     1861 aaaaattcaa gagagaatgg ctcaaaaaga agatattata aattccaccc aaagcgccac
     1921 ccaaggaatt aataagcaag acagcaaaga gcataaaacc aaagtttttc tgtccacttt
     1981 taagaaaaac gagacgtaaa tttcggtaaa ttgttaggaa ctggtctttg atagaaagct
     2041 tctcattttt taagttttca ccatcagcag atgacattga caggctcaat ttgctttttc
     2101 ctaaaaagag gatagtggct gatacgagga aaaagcaagc attaattccc gcaacgaggg
     2161 aaaaattgtt gaccgataga gctaagagcc agactccgaa agcttgacca ccaatagctg
     2221 aaatataggt gatgaactgt gaaaaagaat aagcctccat cagatcatct tcagctactt
     2281 tttccttaat aagaggcata cgcaggccac ctgcaaaatc actgatgata tcactaatga
     2341 cattgatcaa acacaggcta gaaaaggcaa agagactagc ttgctgaaca actagggctg
     2401 ctagaaaaat agaaccgcct gaaacaaacc gctatagacc atccatttga ccttgtccct
     2461 cgtgtaatct gcccgaatcc ctgcaaaaac tgtaaagaag gtcggaagaa tcatgacaat
     2521 attcgccata gcaacagcaa aagatgcttg tgacaaggtc gatgcataga cgataaagac
     2581 caggttgaaa atcgaagcac caaaagcatt gaagaagcgt gacaaagtca ataggcgata
     2641 agcttgattt ctaaacaata gtttcataga taatctccct ccttgttcta agttctacat
     2701 caagaatagc tttagtatag cacttatgaa tttgcgaaac gattcttttg aatatatgca
     2761 acaaataaaa tgagcaaaaa acgatagaac ttttgtaatg acctgagaaa gggcaacagt
     2821 tctatcgctt caaatttttt cttagtttgc agatattcaa cattcggtcg tatttctata
     2881 gtattcggca gatttattac agccaagcat ctcaaaaata cggacagcat cctccatctt
     2941 tttctggcct tccttgactc taccttgctt gctatcaagg agaccttctg cccacagata
     3001 aacaattcgg aaataggtct cattttcctt gtagaaatgc tcttcgataa cacgtttaaa
     3061 ataataggca ttggtaaatt tttcacactc aatactagct aaaaagccat tcaatagtat
     3121 agtatgaaaa aagtttcgat taccagacat ttccattaga aaatcagatt tacgtaccat
     3181 ttctcgtaca tatctagtaa aaagagaaac agataaaaat ggagaactga ctgaaaataa
     3241 attgagttca tagattcccc agatctcggt agaaaacaaa taatcatgaa ggacttttcc
     3301 ttcctctgct gttaactcta ccctttcatc tatgctcttc atataagact tgataataat
     3361 ggcatttaga atatgtttct gtttgttgta agaatgggca tgcttttgat actccctgcg
     3421 atataagtcc tcaagaggtg ctatattctt tggttccaag acatctgtaa tttcttttct
     3481 caactcagaa tctgtatcat actggaaacc tctcgccaga aagaggatct cctccacact
     3541 ggcagatata ttttccagag caaatagaaa cttttccacc aaaagctcac tctgacctgt
     3601 ttcaaaacgg gacaacatag acggcgaaaa ttgtcctccg gttgcttgtc tcagtgagat
     3661 atttcttgac tctcgtaatt gtctaaagac ttttccaatc tgctccatag acttcccctt
     3721 gattccgtat tttcttcatt ttatcatatt tttcagaaaa ttcatcaaaa acttgccaaa
     3781 ttgtcagaat tatgagaaaa tagaggatat ttatcacgtg gagggactgc tatgagagac
     3841 gatatcaaaa tcaatgaccg tgctttggcc ttgcaagacc aaattatcga aaaactagag
     3901 aaagtttttg atacagatgt ggaattggat gtttacaatc taggtctgat ttatgaaatc
     3961 aatctggatg aaacggggct ctgcaagatt gtcatgacct tcaccgatac tgcctgtgat
     4021 tgcgccgaaa gcctgcctat tgaaatcgtg gcaggtctga aacaaatcga gggtatcgaa
     4081 gatatcaagg ttgaagttac ctggtcgcct gcttggaaaa tcacacgaat cagtcgctat
     4141 ggccgtattg cccttggact accacctcgt taagcaggcc aatcactttt aaagatgaaa
     4201 aaagcaagcc gaaaatggct tgctttttga tactcaatga aaatcaaagg gcaaactaga
     4261 aaactagccg caggttgctc aaaacactgt tttgaagtta tggatagaac tgacgaagtc
     4321 agtaaccata cctacggcaa ggcgacgttg acgtgatttg aagagatttt cgagtatgag
     4381 tttatttttt acctgacttg tccatattcc agaagtctgt cacggctccg cgtgaagcag
     4441 atgatacgat gtgggcatat ttaccgagga caccacggct gtaaagtggt ggcaaggttg
     4501 tttctgcctt gcgtttttca agttcttctt cggatacggc catggaaatt tctttggtat
     4561 cttggtcaac cgtaacgata tcgccggtac ggagataggc aattggtcca ccatcctgag
     4621 cttcaggagc gatatgtcca acaaccagac cataagtacc accagagaaa cggccgtccg
     4681 tcaagagggc caccttatct ccctgacctt taccaacaat cattgaagaa agtgacagca
     4741 tctcaggcat accaggacca cctttaggtc caacaaaacg aacaacgact acatcgccat
     4801 caacgatttc atctgtcaga acggcctgaa tcgcatcttc ttctgagtca aagaccttag
     4861 ctggcccaac gtgacgacgc actttaacac ctgatacctt ggcaactgca ccgtcaggag
     4921 caaggttccc gttcaagatg ataagcggac catccgcacg ttttggattt tcaagtggca
     4981 tgataacttt ttggcctgga gtcaagtctg caaagtcagc caagttttca gctacagtct
     5041 taccagtaca tgtgatgcga tctccgtgaa ggaaaccatt tgccaacaag tacttcataa
     5101 ccgcagggac accaccgact tcgtagaggt cttggaagac atactgacca gatggtttca
     5161 agtcggccaa gtgaggcaca cgttcttgaa tcgtattgaa gtcctcaagt gacaagtcaa
     5221 catttgcggc atgggcaatg gcgagcaagt gaagagtggc gtttgtagaa ccaccgagag
     5281 ccatcgttac agtgatagca tcttcaaagg cttcacgagt caagatatct gatggtttga
     5341 gaccaagttc caacatctta acaacagcac ggcctgctgc ttcgatatct tctttcttat
     5401 cagctgattc agctgggtga gaggatgacc ctggcaaact catccctaga acttcgatag
     5461 cagttgccat ggtattagca gtatacatac caccacagcc accagggcca gggcaggcat
     5521 tacattcaag acgtttcacg tcctcagctg tcatgtcacc gtggttccat tttccgatac
     5581 cttcaaagac agaaaccaag tcgatatctt taccatcaag atttcccggt gcaatagttc
     5641 caccataggc gaaaatagct gggatatcca tattagcaat agcaatcata gatccaggca
     5701 tgttcttgtc acagccaccg atagcgacga aggcatccac gttgtgacca ctcatagccg
     5761 cctcgatgga gtccgcgatg atgtcacgag atgttagaga gaaacgcata ccaggcgttc
     5821 ccatagcgat cccgtccgct acggtaatgg ttccaaactg tacaggccaa gcgcctgcag
     5881 atttgacacc ttctttagcc agtttcccga aatcatgcaa gtgaatgtta catggtgtat
     5941 tttccgccca agtcgaaatc actcccacaa tcgatgtttc aaagtcctta tctgtcatac
     6001 cagtcgcacg aagcatagca cggttaggtg atttaaccat gctgtcatag atgctactgc
     6061 ggtgacgttt atctaattca gtcatcttat ccctcccatt tcagttttta ctattatagc
     6121 acaattttcg catgaagaac agaataaaat tcttgaattt tcagaaaatt ctatacacat
     6181 gtgaaatatt taaaattaaa aacaacaaag cggattagtg cactttctga tgaccagaat
     6241 atgcttttta atccgctttc tttaaataac gtactgtaat ttttacagaa attctttcaa
     6301 ataagtgtat ttaacatcta tcttgcatta taaatttcta gaaccttctc ttttatattc
     6361 gattcactca aaccatactc attaagaaga taatccattt tccctacttg accgaatctt
     6421 tcttgaacac ccatccgatg aatttttgtt attccatcat cagagaataa ttcacataaa
     6481 gcactgccaa ttccacctat ctgattgtgg ttttctacag taaatatagt ttttccactt
     6541 aacattgttt ttatctgttc tggtatcggt ttgattctaa ataaatctat cacacctact
     6601 gaataaccta atttagacag ttcatctgca actcgaatac ttggagcaac cattatgcca
     6661 gaagcaacga ttacaagatc ttcaccatgc cttaactcaa tgtagccttt agaaaaatct
     6721 tctccacctt gatacacagg aactggagct tttctaattg ttcgaatata ttttagtcct
     6781 tttaagtcta atgtctggtt caatatttca cgaaattgga tatcatcagt tgcttcgaaa
     6841 atgattgatt taggaattaa acgtaacaat ccaatttctt caaatggcat atgtgttcca
     6901 ccattcatct ctgccgttac tcctgcatct gatccaatca cagtggcatc caattgtgcg
     6961 tatccaagag aaataaataa ttgatcaaat actcttcgtg aagcaaaagg accaaatgta
     7021 tgaagataag gtctaaaccc ctgaatagac aagcctgctg caaggccgac catttctgct
     7081 tccataatcc caacattcac ataacggtct ccaaagtcct tttcaagatt attagtagcc
     7141 atcgaacttg acaaatcggc ttctaagact actatatcag aatcactttg attagcctct
     7201 aaaaggaagt ctctatatac atgccgtaat tctttcgtac ttctcatcat tctgtttcct
     7261 ccaattcctg acttaatctt tctacaactg aagttaacat ttgtttctcc tctacagtag
     7321 ggcgaagatg atgattggat ttcatttctt ccagctcttg aaccccttga cctttaatag
     7381 tatctaatac aatgcactta ggtgatgaat tatttgactg ttttaattgg acaatccctt
     7441 cataaatttc tctaatatct gaacccttga ccctaatgga ttcaaatcca aatgctgaaa
     7501 atttttctac gaaatcacct ggattacaaa tatcctttgt aaaaccatct aattgttttt
     7561 tgttatcatc aacaaataca attaagttgg ataactgttg atgagaagca aactgtatag
     7621 cctcccaaca ttgtccctca tttaactcac catctccaac aatagcgtaa gtataaaagg
     7681 gactctttct tattctctga ccatatgcaa gtccagttgc aacactaatt ccttgtccta
     7741 aagagcccgt tgtcatatct atgcctggcg ttagatttct atcaggatga gacggtaatt
     7801 tggttccatt tgtatttaaa gaatataaga attctttgtc aaagaaacca ttcaaataga
     7861 gtgtactgta tagagctggt cctccgtgac cttttgataa tatgaaataa tctctatctc
     7921 gtgctgcaaa tatttctgga gtcattggca ttatttcacc ataaagcacc gctaaaactt
     7981 ctacgataga cagacttcct ccgtaatgtc cgaatccaag atgattcaat gttctaagag
     8041 tatttaatcg gatgttagtc gcaaattttc ttaacccatc ttctctattt ttacttaaaa
     8101 tcatccctta ttcctccgtt gcagatggct ttttaataaa ggatactcca aacataactg
     8161 ctagaataag aacaagacca atcacaatgc ctgcttgtga gccaaattga tttaacattc
     8221 ctaaaataat tcctgataga ccaaaatctg catctgagaa agttgatcct tggaaaccaa
     8281 gtcctcccaa aactggcatt aaaaagactg gaagaaaact gattaaaata ccttgtaaaa
     8341 atgctccaat agtggctcca cgaacaccac cagatgcatt cccaatgaca cctgcagtcg
     8401 ctccacagaa gaaatgaggc acaacacctg gtaagataac aaccgttcct gaagcaatca
     8461 taattaccat acttactaaa ccaccaacaa aactagagat aaatccaatt agaactgcat
     8521 tgggtgcata agtataaaca atcggacaat ccaaagcagg ttttgaatta ggtacaagac
     8581 gctctgaaat acctttaaag gctggaacaa tttcgcccaa aataaggcga acacctgcta
     8641 aaataacaaa tacccctgct gcaaattgac ctgctaattg taaagcataa actagaccac
     8701 ttgtaccact actgatttct ttttctatat attctgaccc tgcaaagata gctacaataa
     8761 tgtaaataac tgccatggat aaagtaatac taacagtact atcacgtaaa aaagctaaac
     8821 tctttggaaa tttaatgtcc tctgttgatt ttgatttgtc accgataagg ctaccagtaa
     8881 aaccactcaa ccaatatccc aaagaactga aatgacctaa agctaccttg tcatttccag
     8941 ttaattgaac catatatttt tgcacaaatg ctggggaaat actcataata ataccgagtg
     9001 ctaatcctcc tagtaagatg agaggcaagc tagtaaagcc agcaactgat aaaatgaccg
     9061 caatcataca tgccatatat agagtgtggt gccctgttaa aaaaatatat ttaaatcgag
     9121 taaaacgagc gattaagata ttgaacacca tgcctgcaaa cataatcatt gcagtagctg
     9181 agccatatgt tgttaaagct acagctacaa ttgcttcatt attcggcaca acgccagata
     9241 aatgaaaagc atgctcaaac atggtaccaa atggatttaa agaattttgt acaattcctg
     9301 caccaccaga tacaactaag aaaccaacaa aggtcttaat tccaccttta ataatatcag
     9361 gtaatttctt cttctgaaga actaatccta agattgcaat taaagctact aaaatagctg
     9421 gtgtactaac aatatccaat atgaacttca tcatgacgct agcctcctat ataagtcctt
     9481 tttcttcaca aagtttagta attaattctc gtagttcatc catatcaata atactattta
     9541 agatacgaac atctccaaga tgactagctg aatcagctag atcacgacca acaatccaaa
     9601 tatcagctgc atttggatct gctccaccta aatcataatg ttcaacttct acatccgaaa
     9661 cattcaaatc actcaataca gattcaatat tcatctgtac cataaaactt gaacctaatc
     9721 ctgaaccaca agctgtacca atttttaaca ttatctaatc ctcctgttta attatcattt
     9781 taatgtcatc atagtttttt gatgatatta aagtttgaac atgattttta tctcttaaaa
     9841 ttgttgttaa atgtgacaaa gcctttaaat gactctcatt atcaatggct gcaatacaaa
     9901 tcaacaatct tacctcttgt tctggattat ccaataaata aatcggttct tccaaaacta
     9961 acattgacat tcctatttca ttcacacctt catctggccg agcgtgagga attgctactc
    10021 ccttccctaa attaataaaa ggtccaaact cttctacttt ttgaatcatt gcctcagggt
    10081 agttctcagt tatcttatct tgatccaaaa gcggtttagc tgctaaacga atcgcctcct
    10141 tccatcctaa tttttgcgaa ctaacctgat aggtttcttt ggtaataagt tgttctagca
    10201 ctggtacaat ttcctttcta tcattttttt ggtaaagata attctttaac gccaatctta
    10261 attccaattc ttgtgtaata attccatatc ttttgacaat attcaggatt tgttcaatct
    10321 caaaatctcc atactctaaa ttcggaaaat cttttaacac tagttctact agttgtattg
    10381 cttgctcttc agtcatcata accgaaacta gataatttgg cttttctgtc tccaccttta
    10441 tggtagaaaa aaccatatca tagtcactac tagctttcac ctgtaaatca tcaatctttg
    10501 aggttcctat aaactcaatt tgaggaaata atgctaatag attctctttt aacatcaatg
    10561 aagaactaac accattagga caaatgattg ctgctttata ccatttttga ggcaaagtat
    10621 ctgctttctt taaataacct ccgaaatgga taacaaaata tgctgtttca ctatcaggta
    10681 tgggattgtc aatagcgtcc atcaagggca tcaaagaatc tttgactaat tcaaataaat
    10741 caggataatg ttctttaaca tgcaatacat attcatttga actaggtagg ccgaacttta
    10801 atctatagta agccggtata aggtggcggc gaagattttc tctcaatcct tccctttgtt
    10861 taaaatgtaa caaagaaata tcttccattc tacttataat agcctctgtt aattgattaa
    10921 agtaaaccgg agcaacatct acttcacctt caaagcaact tgataataaa acggtgatat
    10981 agcgataatc atcctcagaa aacaccgtat ctataattcc caaatcaacc actgtatcca
    11041 ataaaatagt ggttatatct tgaataacag gagatactaa tgtctctgaa agacatactc
    11101 tttcaacatc cctttgatac ctacacagaa tgaatactaa accgaaaagg taaactttta
    11161 attgattaac aataggtact agctgtagct tctcataata atctttaact acctgatcaa
    11221 tcaaatcata agttaatgaa tacccccaac tggataaaac ataatccaaa ccccaaatcc
    11281 ctatggagga ttccagcaac tcactaacca tttgaaaagc taagcggtgc ttattccact
    11341 ctgaaccgtg taaagtataa ccttttgctc tactgtaccc tagctccaaa tcattatcta
    11401 acataatctt tcttaatgat tgaatatcag ataaggttgt attcttactt actttcaaaa
    11461 agtcttggta atgactattc gatataaaat ctaatcggca aaaagtgtaa agatagatta
    11521 aagctaagcg agtcgacttt ggtaaaacca attcatccga cttaataata tctgtcaaag
    11581 actgcttcgt acgatttgat aaactatagc gaccttgctt tttatccagc actatccctt
    11641 tattagctag ataaggcact aaataatcta ttccttcttt gacttccttt ataggtaagc
    11701 tcaccttaac agataattca tataatgata gctcacaatg atccatcaaa gtcatcaaaa
    11761 taactagtgc tctataatca aacatttctc ttcctttcta actacaatta tagtgtaaaa
    11821 attataagaa taaaagagta tttgagcaca atataatctc accttttaaa agaaattatt
    11881 gggctgaaag tttttagtga ttatttttca actttctatt gactttataa attaaagtga
    11941 tatcatttag atatcaaata acaggaggtc attcttatga ctgaaaaact aatcaattca
    12001 aaaccaaatg gtgtattcgc attgattctc attgagttga caatcgtact tggtatcttt
    12061 atatttataa tgggggttgg ttcggaaaac atttttggaa ttattatcgg acctttacta
    12121 atcgtaattg caggtctagc tcatgctggt ttaaaagttg tcaaacctca agaagctctg
    12181 gttctgacac tctttggtaa ctatacaggt accatcaaag aacctggctt ttactttgtc
    12241 aatcccttca gcgtagcagt caaccctgca aaccacactc gacttggaca aagtggtgat
    12301 gttagcacaa aatctccttt tttaggagct aaatcatcaa atgacaatga tgtaaatctt
    12361 gaaattggca agaaacagat ttccctcaaa gtcatgacct tgagcaattc tcgtcaaaaa
    12421 atcaatgatt gcttaggaaa ccctgtagaa atcggtatcg cggtaacttg gagagttgtc
    12481 gataccgcta aggcagtctt caacgttgat aactacaaag aatatctttc attgcaatgt
    12541 gatagcgccc tccgtaatat tgtccgcatc tatccttacg atgtgtctcc taatgtggat
    12601 actacgggtg atgggcaagc agatgaaggt agtctccgtg gctctagcga aattgttgct
    12661 aaccgtattc gtgaagaaat ccaaagtcgt gttgaggatg ctggcttgga aatccttgaa
    12721 gcacgtatca cttacctagc ttatgctcca gaaattgctg ccgttatgct tcaacgccaa
    12781 caagcatctg ccattattga tgcacggaag atgattgtag atggtgctgt aggaatggtt
    12841 gaaatggcac tagaacggct caatgaaggg gaattggtag aacttgacga agaacgaaaa
    12901 gctgccatgg tttcaaatct ccttgtcgtt ctttgtggca atcatgatgc acaaccaatt
    12961 gtcaacacag gaagccttta ctaaagatgg ctgaagctaa aaaaaagcag atcccccttc
    13021 gactctcaac aaagttatac gctgcactcg catcatgggc agaagacgac tttcgttcag
    13081 tcaatgggca aatcgaatat ctcctcacag agtgtgtcaa gcaacggaag aaagacggaa
    13141 aatacgtatc agaaaccatt gacgaaccat ttgagattga tatttaacac cttctcctgt
    13201 ctgccgtgtc ggataggaga attttttcat ctttttttgt caagtaactt tactttacaa
    13261 aaaaaatgtg ttatcctagt atggttgatg aaaatcagta gattgaatcg aatataaata
    13321 cctaaaggag aaatcaaaat ggcagtacct gcacgtcgca cttcaaaagc gaagaaaaac
    13381 aaacgtcgta cacactacaa agtaacagct ccatctgtaa actttgacga aactactgga
    13441 gattactcac gttctcaccg tgtatcactt aaaggatact acaaaggacg taaaatcact
    13501 aaagctgcat cagctgaata atagaaggga gataccatgc gcgtaaatat tacacttgaa
    13561 cacaaagaat ctggtgaacg cttgtacctt acttctaaaa acaaacgtaa cactccagac
    13621 cgccttcaat tgaagaaata ctcaccaaaa cttcgcaaac acgttgtgtt tacagaggtt
    13681 aaataagcat tcaatacgaa tcaatacaga agaaaaacgc ttgatcagcg ttttttcttt
    13741 ttgttaattt cactacattg taagggaaat cattcgaatg actacatttt tcactacctt
    13801 ttttgaaata agaatacgga ttcggacaat taaaagcaag aggaactcaa atgtaagttg
    13861 gggttccttt tttgtatatt tttcaaataa cgcctctaca ctatttgtag caaattcacc
    13921 aactacagtt gtatcttagt taaaataagt tagaatatgt aagtgagtac cagatatacc
    13981 aagacatcgt caccatctaa ggtatattca aaatacaaaa gttgaccaac tagatttctg
    14041 aatatcctta tatatccatt cttaaaattg gtttaaatag cgtagtcttt taaactagtt
    14101 ttgagaatcc aaaaaatctt cctacatatg taagaagatt ttttagttca gaatgattag
    14161 atttagctaa tggataccta tcctacccac tcaccgttag cattgactcg atagccatcg
    14221 ggtgtagtcg tgtttactaa taaatctcct gaattatagg tatagtacca tttaccagaa
    14281 actgtaaccc aaccagtagc cattgaacct gattcattta agtaatacca taagcctttg
    14341 tctttaaccc aaccagtagc cattgaacct gattcattta agta
//
LOCUS       DAVBCR010000054        14321 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCR010000054 DAVBCR010000000
VERSION     DAVBCR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.054"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(114..1964)
                     /locus_tag="VQ508_001497"
     CDS             complement(114..1964)
                     /locus_tag="VQ508_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2490473.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2032..3171)
                     /locus_tag="VQ508_001498"
     CDS             complement(2032..3171)
                     /locus_tag="VQ508_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2490474.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3229..4395
                     /locus_tag="VQ508_001499"
     CDS             3229..4395
                     /locus_tag="VQ508_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2490475.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VQ508_001500"
     CDS             4534..4860
                     /locus_tag="VQ508_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2490476.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VQ508_001501"
     CDS             4847..5440
                     /locus_tag="VQ508_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2490477.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VQ508_001502"
     CDS             5433..6344
                     /locus_tag="VQ508_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2490478.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="VQ508_001503"
     CDS             6407..7471
                     /locus_tag="VQ508_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490479.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7543..8403)
                     /locus_tag="VQ508_001504"
     CDS             complement(7543..8403)
                     /locus_tag="VQ508_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2490480.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8525..9511)
                     /locus_tag="VQ508_001505"
     CDS             complement(8525..9511)
                     /locus_tag="VQ508_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2490481.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9783..11258)
                     /locus_tag="VQ508_001506"
     CDS             complement(9783..11258)
                     /locus_tag="VQ508_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2490482.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11362..12285)
                     /locus_tag="VQ508_001507"
     CDS             complement(11362..12285)
                     /locus_tag="VQ508_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2490483.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12299..13228)
                     /locus_tag="VQ508_001508"
     CDS             complement(12299..13228)
                     /locus_tag="VQ508_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2490484.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13547..13897)
                     /locus_tag="VQ508_001509"
     CDS             complement(13547..13897)
                     /locus_tag="VQ508_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490485.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         4309 a         3070 c         2629 g         4313 t
ORIGIN      
        1 acggcaaggc gacgttgacg tgatttgaag agattttcga agagtataag tctctacata
       61 ataaaacgca tattaccaag gttatttaac ttgataatat gcgtttttct tttttattca
      121 atgtgggttg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
      181 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
      241 acgaaaatct tatccataac ttgattatca acgagttcgg ttgataccaa gagttcttcg
      301 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtgtgg
      361 ccacttagaa gcaccatctt cttggccaag tcataaatct tgactggttt gcccatatca
      421 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
      481 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
      541 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
      601 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
      661 gccacgcgct tggttgctcc cataacattt ggtggattga ctgccttatc tgtcgaaatc
      721 ataaccatct taggcacctt ggcttcatca acggctctag caacattgta agttccacgg
      781 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggct
      841 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
      901 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
      961 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     1021 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     1081 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     1141 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     1201 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     1261 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     1321 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     1381 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     1441 gttggatgtt ggtaactatc cataaagaga gccccaccat caccggcacc aatcaagaag
     1501 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     1561 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gataaaacgg
     1621 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     1681 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat actcgcgctc
     1741 aagccccaaa aatcaaccat caattgatag aacaggaagg ccaaactcgt atagataatg
     1801 tagtcaacag gtgctggatt aatcagccca taaaataaaa tataagatac aatgatggaa
     1861 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     1921 atttccacca gatcaatcac ataatctgtg agttttttat tcatagaaat ttactcctcc
     1981 ttaaaatagt tctactatat tgttattata gcacatttta atttatttgg cttatttttc
     2041 caaataaatt actgttccct tgtactcctt cttccattta aatcctctaa ttaaacctac
     2101 aatcgtccca aggccataag caaagtgaat ggaaaataaa agaaagggca tcacaattag
     2161 aaatccattt ttatgtttta ataaagtcag caaagtgagt aatgacagta atgataaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacataa tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct ttgacaatcg tcggtctagg
     2701 ccctccacag acaaattcac cttgttgaat aacagccaca ttgttcatta caaaactctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggccc cctacagaat gcttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgcatct tcttttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaattttccc ttccaccatt ctatcataat
     3121 tttcaagact tttccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga gatattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccga
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat cctcctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgccat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctattcc actcttgggt accagctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatggaca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagtggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaatcctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg taaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttggaaaaaa
     6541 tactcaagta gaggaggaag tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga
     6601 gaaaattgaa actcctgaag gcggtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttacg gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aaattcaatc ctgtcatcct tggcaggatt
     7501 ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt cttgtgctaa
     7561 tgttttaaaa tctttgtcta atatgggttg cacttctatt tgattggcgt ctagttggtg
     7621 gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag caatggcttc
     7681 atagtcttct ggtttagagc catctcctgt gatttgaacc aagtcaatct cccactgcac
     7741 ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc tcggtaaaac
     7801 tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc catcctgata
     7861 gaaggaaact ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa
     7921 ctgagccaaa ccatctgcct ctaaaaagct tttcaagttc tcacgactaa agaaaatagg
     7981 attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat aggcttgaat
     8041 acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat cagtccctgt
     8101 ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct ctgttagtag
     8161 gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct taaaaagaaa
     8221 ttgtgattgc cccactatcg cacactcaat atactcgaga cgattaagag gctgacggag
     8281 atagactaca tcatagatat gtgactccaa accgtctagc aatgccaaga tcgacttggc
     8341 attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc
     8401 cataactgga ctcctttgaa atcgcttaca tacagtatac cttatttttc tgaaagatac
     8461 agaaacaaac tttagatttt tgagtacaaa gcatagaaaa acagcccctg aggactgttt
     8521 gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg cgctcgatca
     8581 cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga acacggcact
     8641 cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag atacgacggt
     8701 tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca aaccagtctt
     8761 tccctacgat ggttgggttg acatggttga catcaacggc gatacgctcg tcaaatacgt
     8821 acatttttcc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc
     8881 ctccgtgcaa ttggccgaca tctttgtagc cttcacggac catccagcct gagaatttct
     8941 cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat ttttccttgt
     9001 tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga atagctccac
     9061 ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg gtatctttat
     9121 caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt tcaagtgggt
     9181 tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc
     9241 gaacaaacat cttcttgaag gcttgttcat tttcttcgtc aatcttgaac cagagttctt
     9301 ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca tagtcacctg
     9361 aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaaga ccgattgatt
     9421 tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga gtataaaggt
     9481 agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct attcttaaat
     9541 tttctaaatt ttttatttat ccatactagt atacctgctt gagaaaacga atgcaattta
     9601 tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat aaggacaaga
     9661 gaaaacatgg atatctatat ccttgtgcca aaaaaaccac tgccctcccc agaccaacct
     9721 gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat caatttagct
     9781 gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt caatactttt
     9841 tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt atctgggtct
     9901 acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat agctgagatt
     9961 tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc tttagcttct
    10021 gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg
    10081 atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta gccatcaaga
    10141 acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc tggaccgtaa
    10201 acaagaccgt tcaaaagttc tgggttcgta ttcaagaggt tcaagatttc cattgatttt
    10261 tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt ttggtttttc
    10321 ttgatgaagt tagtgattgg tttgatttgg atatctttgt tggcaacacg tgaaagcaag
    10381 ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt atcttgttga
    10441 aggtcaaatg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc ttcatagaat
    10501 ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt cgtaacggtt tacaacttta
    10561 gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa
    10621 ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg agctttttct
    10681 ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga aatatcgata
    10741 ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc tacgttggct
    10801 gcaactggta cagcataaat cttaccattt acagtattac ccttgatata agctggatca
    10861 agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc agcataagca
    10921 cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt ttcaccagat
    10981 gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg gatatccaat
    11041 ttggcaccaa ctttttcttc tatgattttg ttggcatttg ctaacaattc atccaagtta
    11101 tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga atcagcagct
    11161 tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccag agcgactaca
    11221 ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc tttttttatt
    11281 tttaaactta taaacaatgt aatgatctta acttcacaca agggaagttg ggaactgaga
    11341 aatgttcttt ttcaataagc actattcttt cacaccaccg atagtcaaac cttttacaaa
    11401 gtagcgttgg aagaatggat acaaaatcgc gattggaagg gttgctacca caaccatggc
    11461 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
    11521 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
    11581 atcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
    11641 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
    11701 agcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
    11761 gaaaaaagaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
    11821 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
    11881 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
    11941 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
    12001 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
    12061 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
    12121 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
    12181 ggcaatcaaa cccgaaatga tactgaagaa gatatctgct ttcttactga aggagtgaat
    12241 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
    12301 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
    12361 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
    12421 gagtcaaacc attaaagaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
    12481 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
    12541 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
    12601 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
    12661 tcgtcgcata gtacatgaca ctgctatatc caaagccttt ccaaatacct aggaaaagta
    12721 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
    12781 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactga
    12841 tgataaccca agacaagaag taagggaaca acatagaagt ttgaaaaatc ttcaccattc
    12901 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
    12961 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
    13021 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
    13081 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    13141 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    13201 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    13261 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    13321 ttaggttaaa ctcctaatta tagaaaaaac tccacaaatt atgtagcaga tttaaaactt
    13381 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    13441 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    13501 agggaactca gcgatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    13561 tcaacttagc aataacctct ttaagattat caatcaactc tgcttcagta tgctcagagc
    13621 ctttttcatc tgccaagaac aaaactgctt tttgaagttc ttcttgagag ttttcaagga
    13681 catccttatc tgctgtttct ttgcccgctg tttgtcctcc atccttagtt gctgactggc
    13741 tttccttaat ggactctagg gaagcaatgg catctttgac tgtttgcaag atatcacgta
    13801 aaccttgctc tgtcaaacta tcatctgcaa aagctttctt agcctctgcc aaaaccagac
    13861 gtgctgaatc tgtggtagga ttcgatacac ctgtcaatga tctcaaaaga ttttctaaag
    13921 tttgagtctg cttactaata ctagactaaa atcaaaaagc attatataac agtgatatga
    13981 aatcaactaa agaagaaatc caaactatca aaacactttt aaaagactct cgtacagcta
    14041 aatatcataa acgccttcaa atcgttctat agtaaaatga aacaagaaca ggacaaatcg
    14101 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
    14161 ttcaatccat tatatttcgt ctgatgggca aatcttataa agagattata gaacttttat
    14221 agtagtttga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaat
    14281 attttagcag ctaaggtgta ctgttataga ttcaatacac t
//
LOCUS       DAVBCR010000055        13901 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCR010000055 DAVBCR010000000
VERSION     DAVBCR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.055"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..88
                     /locus_tag="VQ508_001510"
     CDS             <1..88
                     /locus_tag="VQ508_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2490486.1"
                     /translation="STTYRNHRKRFGLRMNLIVGIINHELGF"
     gene            239..715
                     /locus_tag="VQ508_001511"
     CDS             239..715
                     /locus_tag="VQ508_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490487.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1389..1781)
                     /locus_tag="VQ508_001512"
     CDS             complement(1389..1781)
                     /locus_tag="VQ508_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2490488.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1759..2220)
                     /locus_tag="VQ508_001513"
     CDS             complement(1759..2220)
                     /locus_tag="VQ508_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2490489.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2183..2443)
                     /locus_tag="VQ508_001514"
     CDS             complement(2183..2443)
                     /locus_tag="VQ508_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490490.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2448..2876)
                     /locus_tag="VQ508_001515"
     CDS             complement(2448..2876)
                     /locus_tag="VQ508_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2490491.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2845..3171)
                     /locus_tag="VQ508_001516"
     CDS             complement(2845..3171)
                     /locus_tag="VQ508_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2490492.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3173..4189)
                     /locus_tag="VQ508_001517"
     CDS             complement(3173..4189)
                     /locus_tag="VQ508_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2490493.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4137..5078)
                     /locus_tag="VQ508_001518"
     CDS             complement(4137..5078)
                     /locus_tag="VQ508_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2490494.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5154..5519)
                     /locus_tag="VQ508_001519"
     CDS             complement(5154..5519)
                     /locus_tag="VQ508_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2490495.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5670..6728)
                     /locus_tag="VQ508_001520"
     CDS             complement(5670..6728)
                     /locus_tag="VQ508_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2490496.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6891..8042)
                     /gene="nagA"
                     /locus_tag="VQ508_001521"
     CDS             complement(6891..8042)
                     /gene="nagA"
                     /locus_tag="VQ508_001521"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2490497.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8195..10012)
                     /locus_tag="VQ508_001522"
     CDS             complement(8195..10012)
                     /locus_tag="VQ508_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2490498.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10113..11255)
                     /gene="tgt"
                     /locus_tag="VQ508_001523"
     CDS             complement(10113..11255)
                     /gene="tgt"
                     /locus_tag="VQ508_001523"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2490499.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11385..12242
                     /locus_tag="VQ508_001524"
     CDS             11385..12242
                     /locus_tag="VQ508_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2490500.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12270..12914)
                     /gene="pcp"
                     /locus_tag="VQ508_001525"
     CDS             complement(12270..12914)
                     /gene="pcp"
                     /locus_tag="VQ508_001525"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2490501.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTLERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(13021..13389)
                     /locus_tag="VQ508_001526"
     CDS             complement(13021..13389)
                     /locus_tag="VQ508_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2490502.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13400..13825)
                     /locus_tag="VQ508_001527"
     CDS             complement(13400..13825)
                     /locus_tag="VQ508_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2490503.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
BASE COUNT         4133 a         3113 c         2437 g         4218 t
ORIGIN      
        1 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgttgg
       61 tattatcaat catgaactag gattctagtt ttgcaggaag tctaataatg aaaataaagg
      121 actaaagcaa taactctaaa tacatctgat ttttatagga tatttcataa aaaaaatcta
      181 catacctaga gtcctattta gttatgtatc ttacataaag agaaaggaat taattatcat
      241 gaaattatct ttgtatagtg aagttgaaca ttaccatttt ttaagttctt attctttaaa
      301 tgatattaca tatactgata ccccaaaaaa tattattgaa aagtctaaaa aagataagta
      361 tagataccca atagtaatac ttaataaaga ccataaagta attggatttt tttgcctaca
      421 cctaaacact ggtcctaaag agtatggcta ttatcaaaat gattatgcgc ttattagagg
      481 attttctatt catgataatt tcagaaatca aggatatggt tctaatgctc tgaatgaaat
      541 atttgaattc atagactcaa ctttaaaatt agaaattaac catattatat tggctgtaaa
      601 tgaaaaaaat atattggctc aaaaagcata tagaaattcc ggtttttctg ttgtgaaaaa
      661 agatttagaa ggaaaaaggg ggaaattaat tattatggag aaaagcagaa actagtaatg
      721 atactagttt ctgcttttct ctaagtctgc tataaattta taacatgttg aaataagatg
      781 tgaataaatt gattgcagaa ttcaaaccaa tttctaacaa tgttttagaa aactcggcgg
      841 actattccag attcaatcca ctatattcta tcttatttgc atattttgag agaaataatc
      901 tatatactaa acttttatgt ttataattca ttcctttcgt ttcactcaag gcacaagaca
      961 gaatgaaaaa gtggtgtgct ctttattttg ttttataata atagtgagaa aacctatcac
     1021 tactacaaat cacggggagg tgaataagtg agtggtatag ccactacctc gcatttttat
     1081 aggtgtcatt ctttccatca agcacaagaa ctgtgactag gagcacgtaa acttataatt
     1141 gaataagcga ctcgtttgtg aaaggaaaga atagtcaagg gataggcaga ctcaaatttg
     1201 gtgaagggaa acccctcatg ttaaaagcca aacttcaatt ttaaaaattt taaaatttgt
     1261 ttcgtattca ctactccttg cctttatatt ggattgatta agagaacctt tcgcgtttct
     1321 agaaactaca gtggactatt ctggattcaa cgcattatag ttgaattgac taggaattct
     1381 ctttcttttc aggcttctct tctgattttt ttctccgctt tttcgcttga atcagtcgct
     1441 acctcttcct ttttatccgt tttctcttct ttgattttga ctgaaggaaa cagaaactca
     1501 tattgactct tatccgtacg aacagtcgtc accaagcttg ttttcttgtt ttgatagctc
     1561 acctgaccta gattaaaaac ctgttcccca ctttcttgct cggctttatc cttggttcgt
     1621 ttagccatgg caaaagcaac caatttttct ttatttaaag catagtcttg atagtgggcg
     1681 atttgtcggg tcaaataaaa ttgcaacaaa agactaaaga tggctgctat ggtgactgcg
     1741 tagaggagaa cacctgcctt aactttcttc tttttccaca cgatagatga actccctttc
     1801 taagcctttt tgaaactgga aacgaaaatg aaccagttga ttgtcctctg taatctgagc
     1861 tgatttgagg ccataaacca taggctgata cccccgtcca ctagcatcgg ttttacgaaa
     1921 atcgtctgac tttgacttac cgatggcgat gtccttgtca tcttgcttca tgtataggcg
     1981 attgccttct actttttcga actgcgaacg gtctaattct gcctcaagtt ggcccacaaa
     2041 caagagccac tccttttgct cgctttgttg ctggtagcga acttctgaaa tgaggagctg
     2101 actcatagct tgaaaaagga gtaatccccc actgatgaca atgagggcaa gcagggattc
     2161 taacaaggta aaagccttga ccttatggct ctttgattgc caacaactgt tctgaaccat
     2221 ggtagacctc caatcccttt tcactagaaa atacctgaat ctcaactccg ttgatgctta
     2281 cctgattttg ccccgtctgc aaggccatct tagctaccct caagacttct tccttttgca
     2341 agatttttgc ttcctcttgc ctattttttt gaatttgtcc caaaaggagg gtcgcaatgc
     2401 tggcaaagat agctagagcg actactgctt ccagtaaaat cactgcccta attttttgtt
     2461 tccttaatgc gtttaatttt tccatttcct agatataatt gatagcgaat cgctccttta
     2521 ctggtctgaa attcaacctt agccagggac gaattgcccc cagctcggtc aaatgtaata
     2581 ctttggcctg atggtgcctg aattccttta ggaactgtca acttttgact gccattgcta
     2641 atcatctgcc catccaagtt cagactagtc ttttgttgac tagctacact gcgtttttgg
     2701 gtttcccgat agagttcttc aaactccata aagaaaatct gttcctctac cgctgcaaaa
     2761 gtggactgga cagagccgga caagcccaag gcaaggatac tcacaagtcc caaaaccaag
     2821 agactttcca gcatggtaaa ggccttaatc attgacttta cgatttgccc ctccattttt
     2881 agtatagtat tcattataag ctttaacctg ttcttccgtg atgcgcccat ctccttgtaa
     2941 cttgctcaga gtagcatctt catttttatc caagctataa agttctgcct ggctttccac
     3001 caccttaaca acagctgctt ttcctttgtc attgactgct tctttttgct tggtcagatt
     3061 aggtacaaag agcaagaaaa gcacgctgat aatcagcaag accaccaaca tctccaccaa
     3121 tgtaaaagct ttaaccttag cttttttcaa gaatgtcatc atttttttca ttttaaaaat
     3181 ttacctccat attttgatac atgggcatga gcattgccgc ataaagtaaa acgataatca
     3241 gtgccacaaa gataaaaacc agtggctgca ccaaattcat ggtgcggttg actcgggtaa
     3301 aaaaggcttc ccaagttttt tcagcataga tttccaactc actacccagc ttggacttaa
     3361 cttccccata ctctatgatg agactcaatt ccttcctaaa gaaaggatag gttcctatcg
     3421 tctgagaaaa ttcacggcca tttttcaggg tttgagccag atcttgaccg atttctttaa
     3481 agagctggga accttgttcc tgcatcattt gaaaaatctg cgtcagctcc attccctgtg
     3541 aaatcatatt cccccattca cgtgcataat aggctgtcaa gtaggtctgc acaaagattc
     3601 caataaaggg aaggcgtgct aagatagaaa agacactcat cttagaactt cttttataaa
     3661 aagttagtgc taaaagggca agcacggaaa caagccctac catgcctaga aaaatttgtg
     3721 gcagattacc gataattcgg gtggcaatat tgctactatc cagttgtggg agcaggtaat
     3781 tccgtagccc cagcataatt aagagaagaa aatccagcaa aatcaaagga taggtcgcta
     3841 cttcaattaa ctttttcttg accttggcca gattgtctag atattcttct atctttccca
     3901 aactcaggtg aagattgcca tgaacttcag ctagagataa ctgagtgaca atggtacttg
     3961 aacatcccaa actttccatc atttctgaga atgatttccc ctgagacaag cctgtacgca
     4021 tctgggtcac acactgcttg tccaacaagg aactcctatc taaaaaggag atagtctcca
     4081 ccagatgaaa accgctagaa aagagattgt taaatagggt gatgatattt ttttgcttag
     4141 ctgtagctaa ttttttccgt ctcagcctga agacttgtga tatgtccatc tttaagaagt
     4201 tggtcaattt gctcattcca cttggctgct tggtgttctt gataatctct gtttgcaaag
     4261 tcaacgattc ctcctccccc gattaatctc tggtagcaga ctccttgcag aacaactgcc
     4321 aattcttctt cactcacacc caactccagc agacgctcat aaacacctcg gatactcttg
     4381 gcgtgaatgg ttgaaaagac tgtcgcacct gtcaaactag ctctgaccac tgcacgcgcc
     4441 gtctcgctgt cacgaatttc tccgataatc aagagatctg gtcgatgacg caaggaaagt
     4501 ttgattagat tttcataggt taggccgatt gcttcgttca actgcaactg aagcatgtcg
     4561 tcctgcttga tttcgacagg atcttcgatg gacataactt gctgtccttt aaagagtgac
     4621 ttggacaatt catgcatcaa ggtcgtctta ccactcccaa ccgggccagc aaaaagatag
     4681 agtccccgtt gcctgtactg cttgcctaat tcttcaatat cctgaaacca aaaatgcaag
     4741 tcctgctcct catcgtgcaa caaacggata accaaactct catgcccccg ataatcgcct
     4801 acagtagata aacgtagaga cgccatcttc tggtcatagg cataatcaca ggaacccagt
     4861 tgactacgtc ttttttctcc cacattcata cccgccacaa acttaaagtg actgataacg
     4921 gctgcaaact tttcaaaatc atagctacca attttacagc gctcgtctcc taccctcata
     4981 tgaagctcat aggcgtctaa cttagggaca aaataaatat cctgcgtccc ttttttccga
     5041 gctgaacgaa tgatttcttg tgcaatttct tgaaccatac ttacctcctc acctatacta
     5101 ttcgcaaagc gttagaaaaa taaaaaaagc aactccaaaa aagttacttt tctctatttt
     5161 aatttcatac ggcaagaacg attcctttcc tgactggttt gtttttcata gcctgagcgt
     5221 agtttttcgt ctgggataac ctgctcatcc tgcaaaagag ccaaagaatg gtattgttgt
     5281 aaatactcat cacattcatc acaccagcgt tggtcttccg ggtcccaaaa aatggtcatc
     5341 aagtcgcttc tacttttata aagaggggat tcttgttcca atctaaacca gcaagttttg
     5401 tagtatttga gagcatcata atactggtca aattttctac ttgctacaat atcttcttcc
     5461 caaccttcta agaaccacca cggttcaaaa tctccgtaca tttctataac acgatacata
     5521 ttcattcatt cctttgtacc gtatattata accaattccg ccagacaagg aaagaaatat
     5581 gctcatttct taattttttg ataaaaaatc cagccatctg atggatgact gaatttctat
     5641 ttgcttatgt acaaaaatat ttttactact taggcttctg agatatcgtt ttcgataatg
     5701 accttaatgg catggtggtc tgctgcctta ctgaagactt cgtaggcttt ttcaatttca
     5761 ctgagtttga aatagtgagt taccaatttt tccggttcaa tcttatgact ttcaagtgct
     5821 ttcaacaatt gtggagtcgt atttgtagat accaaaccag ttgttacatt gatgttgcga
     5881 atccaaagtt tatctaaatc gaattcaact ggtttaccat gcacaccaca gttggcaacc
     5941 gttccgtcta caccgataat cttttgacag aaatcaaatg tttcaggaat ccctacagct
     6001 tcgatagcga catccacacc acgaccatct gtcaaatcat aaatttcttt aatggctttt
     6061 ttagggtctg aagaattaac cttatgagtc gcaccgaatg atagggcagt ttccaagcgg
     6121 ttatcgtcta ggtctaccat aatcaattta gctggtgaat agaattgggc tgttaaaaga
     6181 gcagccaatc caactggacc tgaaccaata atggctacgc tgcaaccagg ttctactttc
     6241 ccttttaaga caccaatttc atatccagta ggcagaatgt ctgacagcat aaccaaagct
     6301 tcatctgaca agtcttctgg agtatggtaa agagtattat ctgcatgagg gacacgtaga
     6361 tattcagcct gcataccatc aatcaagtga ccgaaaatcc agcccccttc gtcttcacag
     6421 tgggcataaa ttcctttttt acagtagtag catttaccac aggcacagac gcaagaaatc
     6481 aagaccttgt cacctttttt gaagttggaa actccttccc caacttcttc aacaatccca
     6541 atcccttcgt ggccaagaat ggtaccactt tggcaagtag gaacatcccc tttgataata
     6601 tggaggtctg ttccacaaat agtggttttt acaatacgca caatagcgtc tgttggcttg
     6661 cgaataactg gtttgtctac atcaacaaaa gaagcaagtc ctggtttaac ataagtatag
     6721 gctttcataa agcactcctc cgttttttta tttgtaatat ttatagttca attgtaagcc
     6781 tttttctttg tttcttcaag ctttttttgt gattttttta actttttatt taccagtaaa
     6841 gtaccctgta taaaaaagca gaagctagtc tctaacttct gccttctctc ttatgcttga
     6901 taacgtttca caccatctag gtaggttgct accaattcca aatctttatc taatacgatg
     6961 aagtcagcgt cgtagccttc acggatttgg ccacagacgt catcgatgtg aacggattta
     7021 gctgggttga agctggccat catgactgct tcatgcggat tcgcaattcc ccattcgacc
     7081 acattcttca aaccatcttt gagtttgagg atagaacctg ccaagttacc tgtcgatttg
     7141 aggcgtgcag ttccatttgc aacgacaact gggaattctc ccaacatata atctccgtct
     7201 tccaatcccc cagctgtcat acagtctgtg ataagagcga tgttttctgt tcctttttgt
     7261 ttgataagaa tttcgcaagc ctttggatct acgtggtgac catcacagat caactctgca
     7321 taggtatgtg gcaattggta catggctcca accataccca attcacggtg agtcaaccca
     7381 cgcattccat tgtaggcatg cacccaaaca ctcgctccag catcgactgc ttttttggct
     7441 tcatcaaaag tcgcgtttga atgtccaaga gcaaccgtca caccttcgcc cgtaactgta
     7501 cgaacaaagt cttccacccc atcacgttct ggtgcaaggg caattttatt aagcaagcca
     7561 tttgccgctt tttgccaaga atgaaactcc tcaacacccg ggtctctcat ataagttgga
     7621 ttttgtgccc ccttaaaagt ttctgtgaaa tatggacctt cataataaat cccacgaatc
     7681 ttagcacctg ttgcttcttt ataatggttt ccaagatttt cagtgactgc aagcaattgc
     7741 tcataagtgg ctgttaaagt tgtgggtaag aaactggtaa caccggtact aagaagtcct
     7801 tcactcatag tatgcaatgt accttcaatg ttgttgtcca tcacatctac acctgcatat
     7861 ccatgaatat gagtatccac aagtcctggg gcaatgctat aacctgtata gtcaatcacc
     7921 tcagcccctt caggaatctg ctctacatgt ttcccaaact tgccgtccac aagttccaag
     7981 taaccacctc gacgaactcc gtgtgggtag aaaaactgat ccgctttaat atagttaggc
     8041 ataatgttaa cctccttaaa agattgattc tacaatttat tatgtcaatt acattataaa
     8101 cctttctttt ttatttgtaa atggtataga cctatttttt ggagatattt taaaagagct
     8161 ggatttctcc aactcttctc ttttttaata caagttattt tgatttaacc ggcttgtctt
     8221 gagctgtctg caaagctgtg gcaatcgtat ctgcatacaa ttttgctcct gcttcgatag
     8281 tgctactctc actcccgaaa tgaacctggt ctgttccagc ccaaatttct ggatgctctt
     8341 tcgcaacttg attccaatct gctatcgtaa tgtaaggtgt cttctctgcc aattctctca
     8401 tataggcagc agccttctca acgatggcat aggtctcttt tgtcttatct ccctcataag
     8461 gagtcaccaa aatcatatgg tgtcccttag gaagattttt cacgatactg tcccagtcat
     8521 ccttgtaatt ctcaggatta tttaccccag tcgcaatgac caccgtctta ggtaaaaatt
     8581 tattctggct attatttagc atgatttcat ttgcggtctt ggttgttacg ctgacctgcg
     8641 cgttaatctg tgctccagga agagctgtct gtagtgctgt atttgccctt aaagccactg
     8701 agtcaccaat taacatagtg ccatcagcaa ttcccaaact gtttgcatct gcccgttctg
     8761 ccatcacctt ggtctggcca atatttgttg cagcttgctt caagccattg acagtcaagt
     8821 ctgtctcaaa cgctcccact tgtggtgcca acaaggtcac catgcagaca atgaaggtca
     8881 agattcctgt acctgctgca agaattgcgt gaatataagg caggggacga agagtttgta
     8941 aaataggtgt gttcttgcct gcaatccaag gttccaatac ataaaatgac agactggcaa
     9001 agccataaga acaaatcaga gtcagtaata cagcaagaag atttgatgtc aactgtgaga
     9061 aaatgatata gaaaggccaa tggaaaagat aaaccgcata gctagtatcc gctaaaaagc
     9121 tgataatctt cggctcctgt atatgatgtg tcttttcatg taagacacgc gccgccagaa
     9181 tcatggcaag agctgcaaga ctggcaagca agaagccgat aagataggca aaaagataag
     9241 tgaatttgac aaagaaagtc aaaagaacta ggaaaccaaa gcctcctcca aaaactacta
     9301 aagtctttcg taaatcccag attttatcca actgcttgac gagggaagtc gtctgacgaa
     9361 cgcctacaat agttgctaac atacttccca aaaagaatgg atagacatga gttaaactgg
     9421 agaaataaac agaggaataa gaggtcacta gaaaactacc aataaacatg gagaagaaac
     9481 tgatcaagaa ggcagtagca gataagagaa agatcatccc cttcaactga ccatttgatt
     9541 tagcttgttt ggataagaac caaactgcca atccccaaag aatatagtag tgaacctcaa
     9601 ctgccaaact ccaattatga acaaacaaat gaggaatgaa ctgagattca taactcccac
     9661 ctgttaagag ttcatagaag ttggtcataa agcctaagac gcccgcaatc tggccaccaa
     9721 ttccagcaac atagtcttgg cgaaccaaga aagtaaaagg catggtcacc aagaccatca
     9781 aaaccacagg tggcacaatc cgataaaagc gtcttctaaa aaatcctatc aaatcaatct
     9841 cattgttctt agaaaattct tcaatgagta gagctgtaat caggaaacct gaaaatgtga
     9901 aaaagacatc taccccgaaa aatcctccag gaaagatcgt ctgaaagaag tgatacaaga
     9961 gtaccaaaag taaacctata atcctaatca aggaaaacca tttaatgcgc atacgagttt
    10021 attcttcctt tcattgtaca ctttattcta tcaaaaaaag aagaaaaatc ctaagagaaa
    10081 acttaggatt tttagcttat atcaattcca ttttagaaat tacgtcctga cttattatag
    10141 ccatattttt ccacaaaata cttacggaat tccaagagat tgtcatccat gatggcttgt
    10201 cgcacttgct tcatcaggtt aagcaagaag taaagattgt ggtagctagt caagcggata
    10261 ccaaaggttt catcagcctt gagcaggtga cgaaggtaag cgcgtgtata gttattacat
    10321 gtgtagcaat cacactcagg atccagtggc gtaaagtcct cagcaaactg ggcatttttc
    10381 acaaccaaac gtccttgact ggtcatacaa gtcccgttac gagcaattcg agtcggtaag
    10441 acacagtcaa acatatccac cccacgaatg accccatcga tcaagctatc tggcgctccc
    10501 acacccatca gataacgagg tttattttca ggcagcagtt gagttgtaaa gtccaagacc
    10561 gcattcatct cttcatgggt ttctcccact gccaaaccac cgatagagta gcctgagaaa
    10621 tccatgctga caagatcgtg agctgattgg cggcgaaggt cttcaaatcc tgcaccttgc
    10681 acaattccaa acaaaccttg gtcatgtgga cgacggtgag ccttcaaacc acgctcagcc
    10741 caacggctgg tacgctcgat cgatttctta acgtagtcat aaggttgata aaactgagga
    10801 cattcatcaa aggacatcat gatgtctgaa cccagattat tctgaataga gatggctttt
    10861 tctggggata ggagcatctt agaaccattt agatgatttt taaaggttac tccttcttct
    10921 gtgatattac ggctatctgc taaagaataa acctgaaaac caccactatc tgtcaagata
    10981 ggctggtccc aattcatgaa cttgtggaga ccaccagcgc gtgcaatgag ttcatctcca
    11041 gggcgaagcc agagatgata ggtgtttgat aggataattc ccgaacccat ctccttcaat
    11101 tcttcaggtg actgagtttt gacagtggct tgtgtcccaa ctggcataaa cataggtgtc
    11161 ggaaaggtac cgtggggagt gatgatttct cctagacgag ctcctgtgtg tttttctttc
    11221 ttaatcaaac gatatttgat tggtgaatct gacatttttt acctccgaag ctgggaaaga
    11281 cagtcccagt tcatacagtg tgcccaagga cacactgtat gattttatca aaaaaagtag
    11341 gaactgtcac gaactatgta taagaactat ggtataataa gagaatgaac tatccaaaaa
    11401 ttgatttaaa aacaattcgt caggagtcaa aacattttca ggctgacacc ccccgtctct
    11461 ttctcctcta tatccttccc agtatgctgg tcatcttatc tggttttctt aatcccttat
    11521 ctcggatcca cgggactgtt ttagaacagc catttttcag catacttggc caaatacttc
    11581 aaacctacct ctttcctcta ctagtctcct ttatcgggac tattcttttg accagttccg
    11641 tctatgcaac actgacactc atgaaagatt ctaaaacgga gccatctgta aaaaacagcc
    11701 tagctctctt tgatgaggag cgcttttctc aaaccttttt gaccctactt ctcaaacgtt
    11761 tctatctctt tttatggagt atccctaatt tacttgggat ttacctcctc ttttatagta
    11821 gctttttagc caaaaaattc gtcaccctac atcctgagtt tccaaatcta gatctctcat
    11881 cagttgaaac cgagcgtttc ctcatggtct ttggactcta ctttcttgca agtctcatct
    11941 tgatcattgt aggaaatatc ctctatattc cacagtatta tgcctattca caggtggaat
    12001 ttcttctctg ttatagtcta gacctaggac aggtcccacc aagacgaatc ctaaaaacta
    12061 gccgttcctt tatgaagggc tacaagttcc agcactttgt cctagattta cagcttcttc
    12121 cttggtactt cctcaattgg attacctttg gcattgctag tttttcactc ctaccttata
    12181 tccaatgcac taaaattatg ttttaccgag cagtattggc tcgaaaacgt ccaaaagctt
    12241 gaaggttttt cccctcaagc ttttttctat caatgagttt ctccgcctac caacttgagt
    12301 tcctgatctc catgttctat catagcgccg attgctgctt ctatccctcg ccgaatatcc
    12361 actaaactca tagttggagt agtcggtctg ttcaccacct gttccatcat ataaggaata
    12421 tgcataaaac ctgccttaac atatggaaat ttcttttcta ccaaatagag agcctgatac
    12481 atcaaatggc tgcagacaaa agtccctgcc gtattggaaa cagaggccgg taagccctct
    12541 ttttttatag cttgaaccat cgctttaatc ggcaaactac taaagtaggc cgaagcacca
    12601 tctgggcgaa tgggacggtc aatcggttga ttatcttcgt tatcagaaat gcgtgcatcg
    12661 tcttgattaa tggccactcg ttcaagtgtc acactagttc ttccaccagc ttgcccaata
    12721 caaaggacaa agtcaggttg ataacgattc atctcttctt ccaatacttg agcagatttg
    12781 tgaaaaactg tcggcacctc tagccaacgg acctcagcac catggatttc agctggtaaa
    12841 cctttaatgg cctccaaagt tggattgccc ttttcccctc caaagggctc aaaacctgtc
    12901 actaatactt tcatttctaa ctcctcaaaa ccaattcaat gagacttttt tcttaaaaac
    12961 aagtataaca tatttccctt attctggatt aaaaaggatt agcgagatta aaatcgacct
    13021 tcaagtgtat ttaaagagta aaatactaat caaggccaaa atagctgatc caccttgctt
    13081 caaaataatt tttttatccg ctgttaaaga gccgtaagtc gcagcaccaa tcacaaataa
    13141 gacaaaaata gtcacaattt ctaaattctg tgagaaataa atgccatacc agagaaagac
    13201 tcctagcaga gccttataaa tcccttgatt tttgaacaat gaacttactg acggatgagc
    13261 caattcttcc ttttccatat taaatacacg actagtcgca tctgatcgcg tagcaatact
    13321 ttccaaataa aaaatgtaaa aatgctccaa agcaacgatc gttgttaaaa taattgtaat
    13381 aattgacatc tatttctcct taaatttcta tattttcaat acccaaaacc aatttattta
    13441 ataaacggct gagttctgtt ctctcaaact ctgttaagat actttccatc tcttccttaa
    13501 ccttgatatg ttgcagaggg ggattcacca ctaactgctc cttggcatac ttcgcagcct
    13561 ctaccaacac ttcccgctga ttttcaggat tacgatgacg ctccaccaaa ccttccgttt
    13621 ccaaaatctt gaaatgccgt gtcaaagcag cctgatcaat tttcaaacgc tcctgaaccg
    13681 ccatttggtt acaaggagaa tgctccagca aaaacagtaa aatctgatac cgtgtcaaac
    13741 taatcccaag ccttttttca aacaattgcg tactcgcttg ctcagacaag cggagttgat
    13801 acagtaaatc ttgaatctct atcatttctt cctcctatcc attgacttat caatagttga
    13861 ccaatcaatt ataatataaa gtatagtgaa atgaaataag a
//
LOCUS       DAVBCR010000056        12531 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCR010000056 DAVBCR010000000
VERSION     DAVBCR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.056"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..196)
                     /locus_tag="VQ508_001528"
                     /pseudo
     CDS             complement(1..196)
                     /locus_tag="VQ508_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(258..707)
                     /locus_tag="VQ508_001529"
     CDS             complement(258..707)
                     /locus_tag="VQ508_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2490504.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(709..1467)
                     /locus_tag="VQ508_001530"
     CDS             complement(709..1467)
                     /locus_tag="VQ508_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2490505.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1582..3561)
                     /gene="pknB"
                     /locus_tag="VQ508_001531"
     CDS             complement(1582..3561)
                     /gene="pknB"
                     /locus_tag="VQ508_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2490506.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3558..4298)
                     /locus_tag="VQ508_001532"
     CDS             complement(3558..4298)
                     /locus_tag="VQ508_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2490507.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4313..5626)
                     /gene="rsmB"
                     /locus_tag="VQ508_001533"
     CDS             complement(4313..5626)
                     /gene="rsmB"
                     /locus_tag="VQ508_001533"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2490508.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5619..6554)
                     /locus_tag="VQ508_001534"
     CDS             complement(5619..6554)
                     /locus_tag="VQ508_001534"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2490509.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6567..8963)
                     /locus_tag="VQ508_001535"
     CDS             complement(6567..8963)
                     /locus_tag="VQ508_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2490510.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9029..9343)
                     /locus_tag="VQ508_001536"
     CDS             complement(9029..9343)
                     /locus_tag="VQ508_001536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2490511.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9368..9994)
                     /gene="gmk"
                     /locus_tag="VQ508_001537"
     CDS             complement(9368..9994)
                     /gene="gmk"
                     /locus_tag="VQ508_001537"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2490512.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10124..11737)
                     /locus_tag="VQ508_001538"
     CDS             complement(10124..11737)
                     /locus_tag="VQ508_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2490513.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11928..12182)
                     /locus_tag="VQ508_001539"
                     /pseudo
     CDS             complement(11928..12182)
                     /locus_tag="VQ508_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(12186..12440)
                     /locus_tag="VQ508_001540"
     CDS             complement(12186..12440)
                     /locus_tag="VQ508_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2490514.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
BASE COUNT         3329 a         2988 c         2484 g         3730 t
ORIGIN      
        1 ttttttaggg cgattgattt ttacttccgt cgcatcaatc attaccgtgt cttcagaact
       61 gagaggagtt cttgaaatcg taacaccacc tttgaacaag agttacttca acccattggc
      121 tccgacggag taagttgctt tcgtggatac caaaatcagc cgcaatttct tcataagtgc
      181 ggtattctcg cacatataga aagcgttgtt aatttattta tctcattttt cagaaaattc
      241 ttttatttct gtaaagtcta cgatactcga tgtgttttta tataatgata cagtctgaga
      301 atcactgttc cgctagccat tccaatagag attaccaaag ccaacatgac aaccaaggtc
      361 gcacttgcca gtgctttatt atagtcccct gtcacaaaaa aggcagttgt tcggtaggag
      421 agataacctg gaaccagcgg tgccaaaatg gccaagataa agaccacagc aggtgtctta
      481 taaagaatac ttaaaatctg gctgacacaa gaaccaataa tggctgcaat gaaggtagct
      541 acaatgacat tggtcggttc cttgagcaag agatagatta gccagacagt catgcccaaa
      601 atccctccag gtaagagcat agaccgttgc acattgagta cgattaaaaa agtgataatg
      661 gcaagaaaac ttgctactgc ttgtaataaa aaggttgtta gtgtcatatt agttcatcaa
      721 taccaaggcg acagaagttc ctgcccctaa agcgagggta atgagcaggg attcaaacat
      781 cttactcata ccagagttta tgtggttggt cataatatca cggaccgcat tggtcaaggc
      841 aatacctggt acaaacggca tgaccgcacc agctataatc aaatctgccg ttgaaggaaa
      901 acctgtgtag cgagcccaaa actgggcaat tatcccaaag acaaaggctc cagcaaaggc
      961 tgtcacaaag ggaattcgga taaatttttc cacatagagg gaaaaggcaa aaccaaataa
     1021 ggtcgccact cctgccccaa gtgcgtcgta gatatttccg ctaaacataa ctgaaaagaa
     1081 aggagcacta aaggtcgcag ccagagttac ctgcaactta gtatagggaa ggggttgagc
     1141 ttgcaaggcc gtcaattgct taaaggctgt ttctaagtca atctgccccc caactagctg
     1201 acgagaaatc tggttcacat cgcagacttt ttcgatgtta taagaagagg aggtcacgcg
     1261 cttcatgcgc gaaatattgg tattttcaat agagaaaaag atagcggcag gcatggcaag
     1321 gacattgcaa tccacaatcc cctgcgaatg cgcgattcga atcatggtat cttctacacg
     1381 atggatttct gagccacttt taaggagaat agtccccgct agcataatca catcaatgac
     1441 ggcatttaat tcttttgatt cttccatgct ttcctccttt tatcaactcc ctctattcta
     1501 tcacaaatcc ggactcaaaa aaaatctttg ccatgaaatc atgacaaaga ttgattactc
     1561 attttgatta tccatctgct tttaaggagt agctgaagtt gttttaggtt tgtagattga
     1621 aatcttgact ctagtcttat tgaggtctac cttttcacct gctctaggac tttgttcaac
     1681 aaccatgcct tctacactac ctgcaggcgc tgtcgtcact tctacaactt ctatattagc
     1741 ttccttaatc ccaacaattt gaatcaaatt gttcttagta aactccaagc tggaaccaat
     1801 gtaactcggc atggcaacac ttgtaacttt tttagctact gtcaagacaa tttgagtagg
     1861 tttactcaca tcataagtcg ttccggcacc tggactttgt ttcataatcg ttcctggttc
     1921 gctttcgctg gactcttctt cctctatctt aatcaaattc tcaggaacct tcttctgctt
     1981 gagttctgag attacttctg tagagttccg tccaatatag ttccctaatt gaatcgtcgt
     2041 agctttttta gctactgtca aaacaatttg agttgccttg ctcaagtcat aggtcgtacc
     2101 ttctggtaga ctttgcttca ggaccgttcc agcctcactc tcattcgact cttcttcctc
     2161 aattttaatc aaattatctg gaactttttt ctcttttaat tccgcgataa catcagaaga
     2221 tttccggccg acataattac taatttggaa ggattgtttg cctgatgaga caaccagatt
     2281 gattttcgtt ccttcttttc gaccagttcc agcgccagga tctgtacgga taatccgccc
     2341 ttcttccacc ttttcactag cctctgtctt ctcctcacca atctcaaaat tggctttttt
     2401 gagcgttgcc ttggcctctg caactgtctg acctgccaca tctggaatgg caatggttgc
     2461 aggagttctg gatagtatcc aaataagaga agctgccacc aatacaaggc tggccaacaa
     2521 aatcaggtaa cgcatcttaa atctatgttt tttcggtgct tgtggttggt aagtttcctc
     2581 tgtcacagcc tggcttgggt ttttgattga tttgtgttct gtctgcgctt gaaccttagg
     2641 aatagatgtc aaggtactct gagaaacctt cggcaaggtc ttggtatctg ccttgctcgt
     2701 ttcatcaaag attaacttac tttcatttct acgattgtag gacaagctac tagacaagtc
     2761 cacatacatc tctgaaaccg agcggtagcg attggtcaac tttttagcag ttgccttgat
     2821 aataacattt tctaaagcct gaggtacaga tggattttct gcaataacgg acggcagggg
     2881 tttctggaaa tgctggaggg caatggtcac cgcgctatcc ccgtcataag ggatatggcc
     2941 tgtcaacatc tcatagaaaa taatccccat ggcatagata tcactctgca cagtcgcctt
     3001 cgaaccacgc gcctgctctg gtgacaagta atgaactgag cccaacatcg agttagtctg
     3061 ggtcagactt gtctctgcaa aggctacagc aatcccaaag tctgtgacct tggcagtccc
     3121 atctggtgtc aaaaggatat tttgaggttt caagtccctg tgaacaattc ctcgagtatg
     3181 ggccaagcgc atagccaaga gaatttgtcc catgatacgg actgcttctt cattagaaag
     3241 aggataatgt tccttgatat agcgtttgag gtccagtcca gccacatact ccatagctag
     3301 gtactgttga ccgtcttcct cgccaatatc tgttatccga acgatatgag gatggtctag
     3361 atctgccata gctctcgctt cacgctgaaa acgagctaca gctatcgggt ccgtctggta
     3421 gttggtcctc agaaccttca ctgccacttc ttccccatct aagattaagt ctttggctag
     3481 gtagacatcc gccatgcctc ctcgaccaat ctgtttgaca atccgatagc gtccggcaaa
     3541 aatcttgccg atttggatca ttctgcatcc tcctcgttca taaaaacaag ggcaaccgta
     3601 atgttgtcta aacctcctgc attgttagca aaacgaacaa gtgtctccgt tttatctgct
     3661 aaaggaatat cactggttac aatatcacga atctcactgc ctgaaatcat gttggtcaag
     3721 ccgtcactat tgagcaagag atagtcacct gactcaagga taactgtccc aaaatcaggc
     3781 tgaatttcat ctttttgccc aatagactgg gtgataatat ttttttgcgg atgagcttct
     3841 gcctcttctg gtgtcaattg accagccttg agcaattcat taaccaagga atgatcgctc
     3901 gtcaactgat ggtattcttc tccacgaatc aagccgatac gcgaatcacc aatatgagca
     3961 tagatagcct gattatcaat aatagcaagg acttccaaag tagttcccat gcctctgtaa
     4021 gcttcatcct gaccaagctg gtgaatcttt tgattttcaa tttctaggta atgggcgaac
     4081 cattcacgca cttcattgac tgtatcgatc tgggtatcaa cccaagctac acccaggtct
     4141 gtgaccgcca tttcactagc gatattccct gcacgatgac ctcccatccc atcagctaaa
     4201 ataatcatgg tacgtccagc tctattgaca tagtggttga catagtcttg gttatttgtt
     4261 cgtttctgac caacatctgt taataatgaa atttccatgt gtcagttcct tcctaatccg
     4321 atatcttgcg aaattgactg atgaagaatc catcacttcc atacaattca ggtgtaatga
     4381 ggatacagcc gtctttcatg atatccttac attcatgttc tagttttacc tgctcgaact
     4441 cgggatgact ctctaaaaag gccttaacga cttgaaaatt ctcctctgag acgatagtgc
     4501 aggtgctata agttattata ccacctttgc ctagtatttg acaaacacta cctaatattt
     4561 ctaactgaat ttcctgcaag gacgcgaaat ctgccgtttc tttattgtat ttgatatctg
     4621 gttttcggcg caaaagaccg attcctgaac aaggagcatc caccaaaatc ttatcaaagg
     4681 aatcctggtc aaaaaactca tgcacctttc tggcatccaa tttttgagtt tgaacccgat
     4741 cttcaactcc cagacgttgg gcattttctt gaattaaatc caacttgtgg tcgtacaagt
     4801 ccagagcagt aacctgacct gtcgtaagat aagaggctat atgggctgtt ttcccacctg
     4861 gagccgcaca ggcatcaagc actcgctcat caccttgtaa atcaagcgtc ggagcaacca
     4921 actgactgga ctcgtcttgg atggtaatgg ctccatccgc aaacaaatta tgccctgcaa
     4981 aatgcccctg ctccttaacc agaccagtgg ttgctaaaag ggaattattc gcctccaaca
     5041 aggcttggat ttcctctttt cgacttaggt ctgttacacg aatactggct ttgtttcgca
     5101 ctaacaggct ttcaaagatg gcttttgctc tctcctctcc gtattcttcc ttgagtttgg
     5161 caactagcca aactgggaga gaataggcaa tggagtcacg cttgtttttt cgcttgatgc
     5221 tagcaatatc tggccagcct tcacgcaaga tacggcgaag gacagcgttg accaattttt
     5281 cactgccttt tttacggagt ttggccaatt ccactgcttc attgaccaca gcatgatctg
     5341 gaatcttgtc caaatagcgg agttggtagg cactcatgag aagaaggaca tagagccagc
     5401 tgtctaactg gtctctgtct tcgataaagt gggataggta ccattccaga gtcagtttac
     5461 gggctaccgt tccatagact agctcggtca ctaagccctt gtctgctgca gagagctgac
     5521 tccccttgag gtgtttattt aaggcaatat ttgaatatgc ttgattcaca aaaacatcct
     5581 ctagcactgc tagagctaaa cttctagccg tttctacttt agtcaccaaa tcgttctcct
     5641 acagtcaatg tacgtccaac tccattgagg aaggaagcaa tgtccatctt aggcttacca
     5701 gctggctgca cttgtttgag ggatagagcc ccttcagccg ttgcgacaat caattctttc
     5761 ttgccgatag agagaatctc acctggattt ccctgacctt ctactggtag ggcttcataa
     5821 atcttaaagc ggtcgccctt aaggaaagta tgggcaacag gccaggggtt cattccacga
     5881 atttggttaa agagttgacg attggttttg ttccagtcca gtttttcttc ctctggcttt
     5941 atatttggag agaaggtaac ctgactcgta tcctgcggtt caggtttgat atcaccagca
     6001 atataggcag gcagagtgtc caaaagcaaa tcacgaccaa ctagcgccaa tttttcaaac
     6061 aaggtgccaa cattgtcctc atctgtgatc ggaatgctgc gacgagaaat catatctcct
     6121 gcatccattt ccttaaccat ttccatgatg gtcacaccag cttcctcatc cccttgaatc
     6181 aaggcataat ggataggcgc accaccacgg tgtctaggaa ggagggaggc atgaacgttg
     6241 acagcaaagt ccatgctatc aaggagtttg cttgggagaa actgcccaaa agcagcagtc
     6301 acaattccat ctgctcctag cttcataagt tcttccatct ctggacttcc agataatttt
     6361 tcaggttggt agatagatag tcctgcttcc ttggcagcct gcttgactgg ggtttcttgg
     6421 ataacttttt tacgaccaac agcacggtct ggctgggtca caacggctag aatttcgtaa
     6481 cggtcatctg tcaaaagtcc ttttaagact gttgctgaaa agtcgggggt ccccataaag
     6541 attagttttg tcatatcttc tccttcttat aaaaattgct gcggctcatg gtcaatgctg
     6601 agacggagct cactattttc ccgttcttga gtcaaggcca agacctggtt gagggtcggc
     6661 cccagctcat cttctaaacg gtatttaatt aaaatctggt aatgatagag gttgtgggta
     6721 cgggcaatcg gttttggcgt tggccccaga atgggactgg tctctgacaa gcctgaccgc
     6781 aaaatgttca tgacttcata ggcacgtttg aaaacctctt cttctttctt gtgagaaagg
     6841 gtaataccaa tcgtgaaata gtaaggtgga tagccgagtt gtcgtctgat tcccatttca
     6901 taggcataaa agccttcgta atcttgatcc ttggcaaatc gaatagcata gtgctgcgaa
     6961 ttgtaggact gtatcaagac ttgacctgct ttttcagccc gacctgctcg acctgccacc
     7021 tgagtcaaga gctggaaggt tctctcagaa gaacggaaat caggcagatt caaggccgta
     7081 tccgcattta gaactccgac tagggtaaca ttgggaaaat ccaaaccctt tgcaatcatc
     7141 tgagtaccaa gtaaaatatc cgcttcccct cgcccaaact ggtcaagcaa ggcttggtga
     7201 ctgcctttct ttcgagtcgt atccacatcc atcctcaaaa tgcgagcttg gggaaagagt
     7261 tctgctagct catcataagc cttctgagtt cccgtcccat agtaacgaat actgcggctc
     7321 ttacagttag gacagacctg aggaatatcc ttcgagaaac cacaataatg gcagttcata
     7381 gtcttggtat ccatatgcaa ggtcagagaa atatcgcagt tgggacaagt atccaccgtc
     7441 ccacactccc gacacatgac aaagctagaa taaccacggc gattgagcat gagaaccacc
     7501 tgctcttttt taaccagacg gtcttggata gcctctagca aaggaggcgt aaagtttgac
     7561 gtctcatttt gtccgatata gtctcgaaag tcaatcactt gaacctcagg gattgtagcc
     7621 aaaggattgg cacgttgggt tagacgtaag tgttgataga cgcctttgcc agcacgtgcc
     7681 cggctctcta agctcggcgt tgcagatcca agtaccagag ttgcttgatt atactgagcc
     7741 cgtaaaatag ctacctctct ggcatggtaa cggggattgc tgtcctgctt ataagccgct
     7801 tcatgctctt catcaataat catgacaccc agatttttca gaggagcaaa gatagcagat
     7861 ctggcaccaa caacaacttg ggcatcgcca cgctccacct tgcgccattc atcatacttt
     7921 tcgccattgg ataatcctga gtgaagaatg gctaccttgt ccccaaaacg tgctataaaa
     7981 cgctcggtca tctgaggagt caaggaaatc tcaggtacca gcaaaatagc tgtcttaccc
     8041 ttatccaggg caccttggat aatctgcaag taaacctcgg tcttcccact tcctgtaatc
     8101 ccttgaagta gaaagggagg ttgagaactg ccaatagaac tcacaaccgc atcacgcgcc
     8161 tgtctttgtt ctggatttaa ctccaaaggt ctacttgctt caattccttc aaaataagca
     8221 gccgagcgtt gaacttcctt ttggactatg gtaacagtac cttgatccac aaagaagttg
     8281 acttgctctc gcgagtagga ctctaacaag ctagccaagg aagcgctctc tggatgagac
     8341 agcagataat ctctcagttc caactttttc ttggcacgtg tagaaatctc aacaccttct
     8401 aattgggcat ggtcaacctc ataccaagac tgggtcttga ccttcttttg atcgactgcc
     8461 tgatattcca gaccaagcag gccttttcta gtcaaacgca tcatttcagc ttgcttggca
     8521 aggtctagtg aagaaaaggc tagcgaatct tctgaaccaa acaggcgtac tcgttcttcc
     8581 tgactcaagc cttccagagg atagagaatc ttgtcatagc tagaattcag aaaccctgga
     8641 agcatggcct tgaggataga gattttgtag gagaagacag atttgcgtaa ctcctcagcc
     8701 agccagagtt gttctggcgt gagaacagga gaaaaatcca gcacctctgc aatatctttt
     8761 aaatcttgct ccatctcttc tccatctgat tgggacttca aaccaagaac aatcccttga
     8821 atcaggcgat tacccttacc aaaaggcaca tgaacccgca tcccaacttc cagcattccc
     8881 tcaaattcct ccggaatcct gtaactatag ggctggtccg tctgcatcaa gggcacatct
     8941 acgataatct tagctagggc catcttctca cctcctcctt gtcagtacat tcttgcaata
     9001 gaaaaaataa gattgagtcc ccccaacctt aaattttttc accatcttct ttttctttag
     9061 caatttgctc tttgattttc ttttcttctt cttctttgcg gcgtttttct tcttcgatac
     9121 ggcgacgcac tgcttcacgt tttccttctg gatctgggtg aattgtaacg tttcctgatt
     9181 cgatttcttc taaagcgcga agagttgatt tttcagactt gaaaccttga gttgctgggg
     9241 cacctgcttc caattcgtgg gcacgttttg cttccaagat tacgagtgaa tattttgaag
     9301 gaaccttgtc gagcaaggta tcaatagagg gttttaacat catttgcttg tacctatttt
     9361 ctaaatttta tcgggtagtt ggagattttg gtaacatctc ctgatagtga ccaatgacac
     9421 gatccacaca gaagtgttct gcttcaatca cacatttgac acgttcagca gctaggggca
     9481 cctgatcgtt gacaatcgca taatcatact cacgcatgag ggcaatttct tccttggcct
     9541 tttcgattcg ttgggcaatc acttctgcac tatctgttcc acgacctacc aagcgatctt
     9601 gcaattcatc caaatctggt ggtgtcagga agataaagac agcatctgga acctttttct
     9661 tgacctgaag agcaccctga acttcaattt caaggaaaac atcgattccc ttgtccaagg
     9721 tttcattgac ataggtcaga ggagttccat agtagttgcc gacatattct gcgtattcca
     9781 acatctgtcc ttgacgaatc agctcttcaa attcttcacg agtacggaag aaatagtcaa
     9841 caccgtccac ttctccagga cgttgtgcgc gtgtcgtcat cgatacagaa tattgaaatt
     9901 ggttttcaga actctcaaaa atctctcttc taaccgttcc ttttccaacc cctgaaggac
     9961 cagaaaaaac gattagtaag cctcggtctg ccattgtgtc tccttttagt caatctgtga
    10021 aataacattt ctctagaata atggcaaaaa gccagattat cctttacagt ctttctatct
    10081 agtgtaacaa aaaagcagta atttttcaac tgctctttct tatttattta gcataatcta
    10141 ctgcacgaag ctcgcgaatc acggttacct tgatatttcc tggataatcg agattgtttt
    10201 caattttctt acgaactttg tgagccaaga ttgtgacttt gtcgtccttg atttttcctg
    10261 gattgaccat gatacgaatt tcacgtcctg cttgaagggc aaagctagtt tgcactcctt
    10321 caaagccgtt agcaatttct tccaaatcat ggagacgctt gatgtagctt tcaagagact
    10381 cactacgagc acctggacgg gctgcgctca aggcatctgc tgcagcgacg ataactgcta
    10441 tcacgctctc agcttcaaca tctccgtggt gactagcaat cgtattcacc acaactgggg
    10501 gttccttgta cttacgggcc aattccatac cgatttcaac gtggctacct tcaacctcat
    10561 ggtcaatggc tttcccgata tcgtgaagga atccagcacg acgggcaaga gccgcatttt
    10621 caccaagttc gctcgccatg ataccagcca acttagcaac ctcaatcgaa tggcgcaaaa
    10681 cattttgtcc atatgaagta cggaactgca aacgtcccat aatcttcatc aagtctggat
    10741 gaaggtttgg cgcaccaatt tcataggcag cagcctcacc gtattcacga atcttattgt
    10801 caatctcttg acggtttttc tcaaccaact cttcgatacg agctggatgt atacgaccat
    10861 ctttgagcaa catttccata gtcatacggg caatctcacg acgaatcgga tcaaatcctg
    10921 acaaggtcac cacttctggt gtatcgtcga taatcacatc gacccctgtc aaactttcaa
    10981 aggtacgaat gttacgacct tcacgaccaa taatgcgtcc cttcatagta tcgtctggca
    11041 gatgaactgt tgagtttgtt gactccgcta catattcacc agcgatacgt tgcatagctt
    11101 gaaccaagat gtccttggcc attttgtcag aacgttcctt gacctcttgc tcagcttcgc
    11161 gaatgcgact ggcaatctcc ctggtcaagt tttcctctgt ctgagccaag ataatatctc
    11221 gtgcttctgc ctgagacaga gcaccaatac gctctagttc tgcttctttt tgtctttcga
    11281 cttcctctaa ttgctcttca cgcgcatcaa ggtttttcgc tctatcagaa atactttgtt
    11341 ctttttgttc aagtgtttgt tctttactcg tcaaattgtc gtccttacgg tcaaggctag
    11401 tagctctctc tgtcaaacga ctttcgattt gtttgagttc ttgacgttct gatttgaatt
    11461 cagcgtccac ttcttcacgg tattttctgg cttcttcttt ggcctccaat agtgcttctt
    11521 ttttaagaga cttgctttca cgtttggctt cattaacaag taaatccgct tcacgctcag
    11581 cttgtccacg taaattagtt gcttcttgtt cagcatttaa aagcatcaac tctgcagctt
    11641 cctgagatga tttcatctta gctgagatgc tgacatatcc aatgactaaa ccaatgatga
    11701 cggcaaaaac agcaatcgca agcgacatga tttccatgtt tttacctcat tttattgtta
    11761 ttccgaatga catacattct tttacattct accataaaaa agtgattttc acaaacctaa
    11821 aatagaatat gttttgggga atttggaaca catttaccaa aataaacttg ttgtttagaa
    11881 atagtagttt agtagagact tgagaaaaag cctactttgc agtagactta gtaatgatct
    11941 ttaaaggaca agaaagccac gctatctcca tccatcatat aaatcaagcg attttctgca
    12001 tcaatacgcc gtgaccaggc tccttggtaa tcatatttga gtggctctgg ttttcctatt
    12061 cctgtaaatg gatcgcgttg aatatccttg attaatttat tgattctttt tagtgttttt
    12121 ttatcttggt tttgtcagta gcaataatct gcccaggcat cttctgtaaa cttgagtagc
    12181 attcatcact cctcaatcac atggacctga gtgcttccag cacggacttg agccattcct
    12241 cgcaaaacct tgtctgataa ctccttattt tgggcgattc tcagggtttc ctgaatactg
    12301 tcccattcgc tctttgaaag aactacaatg tcctcatctg gatttttatt gaccaccgtc
    12361 aaaggctcaa attcatcatt taccttcttc atgtagtcct ttaaatgatt tcggaatgtt
    12421 gagtaaagga ctgcttccat aaccatacct cgttttagct cttttccact attatacacg
    12481 aaaagaaaga aattgtcagg aacttgtaca agattttctt ttctatctat t
//
LOCUS       DAVBCR010000057        12469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCR010000057 DAVBCR010000000
VERSION     DAVBCR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.057"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ508_001541"
     CDS             complement(251..433)
                     /locus_tag="VQ508_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490515.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ508_001542"
     CDS             complement(649..1881)
                     /locus_tag="VQ508_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2490516.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ508_001543"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ508_001543"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2490517.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ508_001544"
     CDS             complement(3048..4217)
                     /locus_tag="VQ508_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2490518.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ508_001545"
     CDS             complement(4403..5707)
                     /locus_tag="VQ508_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2490519.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ508_001546"
     CDS             complement(5824..6978)
                     /locus_tag="VQ508_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2490520.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ508_001547"
     CDS             complement(6975..7526)
                     /locus_tag="VQ508_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2490521.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ508_001548"
     CDS             complement(7519..8655)
                     /locus_tag="VQ508_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2490522.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ508_001549"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ508_001549"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2490523.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ508_001550"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ508_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2490524.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ508_001551"
     CDS             complement(10172..10903)
                     /locus_tag="VQ508_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2490525.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ508_001552"
     CDS             complement(10905..12350)
                     /locus_tag="VQ508_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2490526.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4192 a         2422 c         1640 g         4215 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaa
//
LOCUS       DAVBCR010000058        12431 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCR010000058 DAVBCR010000000
VERSION     DAVBCR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.058"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            249..509
                     /locus_tag="VQ508_001553"
     CDS             249..509
                     /locus_tag="VQ508_001553"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490527.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(613..1083)
                     /locus_tag="VQ508_001554"
     CDS             complement(613..1083)
                     /locus_tag="VQ508_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2490528.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1100..3373)
                     /locus_tag="VQ508_001555"
     CDS             complement(1100..3373)
                     /locus_tag="VQ508_001555"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2490529.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3720..6821
                     /locus_tag="VQ508_001556"
     CDS             3720..6821
                     /locus_tag="VQ508_001556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2490530.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6903..7910
                     /gene="pfkA"
                     /locus_tag="VQ508_001557"
     CDS             6903..7910
                     /gene="pfkA"
                     /locus_tag="VQ508_001557"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2490531.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7967..9472
                     /gene="pyk"
                     /locus_tag="VQ508_001558"
     CDS             7967..9472
                     /gene="pyk"
                     /locus_tag="VQ508_001558"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2490532.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(10204..10429)
                     /locus_tag="VQ508_001559"
                     /pseudo
     CDS             complement(10204..10429)
                     /locus_tag="VQ508_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11277..12149
                     /locus_tag="VQ508_001560"
     CDS             11277..12149
                     /locus_tag="VQ508_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2490533.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            12162..12326
                     /locus_tag="VQ508_001561"
     CDS             12162..12326
                     /locus_tag="VQ508_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490534.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPRKNETFIGYIQRYDIDGN
                     GYQSLPCPQN"
     gene            complement(12295..12431)
                     /locus_tag="VQ508_001562"
                     /pseudo
     CDS             complement(12295..12431)
                     /locus_tag="VQ508_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3800 a         2165 c         2656 g         3810 t
ORIGIN      
        1 taaaataggc tccataatat ctatagtgga tttacccact acaaatatta tagaaccctt
       61 ttttattgac agtatcagga tatctttttc aaatttttgg tttgttagat aatgaaaatg
      121 tttctatcaa tctagattta ggattaataa atcgtaaatg taattataaa gaaaaagata
      181 agttaaaaaa agatgtatta gcaagagttg gtctaactca tattcatcta taacaaaaga
      241 tatatgaatt gtctggcggt gaaacaaaac gtgttgcttt agcgaaaatc attcttaaaa
      301 atccaccatt aatattcgcg gatgagttga ctgcatcact cgattcgacg acttcacaag
      361 gaattatgaa attgttatta ggtatgaaaa ataaaaatcg tctaatcatc attgcaactc
      421 ataatcccga tatttggaat caagcagatg aagttataaa tttaaatcaa ttattacagt
      481 caatatatat cttctatatt ttaaagtaat tttatctgaa tccgttaaga gactattgtc
      541 ccaggctctt tctgatgtta aagactttct accaggtttt ttaaaagcat aattgttagt
      601 tgtagtcatt tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa
      661 gcacttttga gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag
      721 ggtagcgata gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag
      781 tccaggaagg gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc
      841 ttccatgtag agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata
      901 ataagcgcta tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag
      961 ggttgcctga gttacttgaa tgtcgttctc agcaagaagg gtttgcaact cagcctgtgt
     1021 atgaatcttg ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt
     1081 cataataatg taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag
     1141 tcaagtggta gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta
     1201 aacggagagt gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacgt
     1261 ccatccattc atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat
     1321 taaggtaacc ttttgtcacg actctttgtg cctctggtct aaatggcaat tcacagagat
     1381 tttccaacat gtgatagcga ccgttgtcaa tggttctagc acttaaaata gctggataag
     1441 gttgtaggta tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg
     1501 tactggattt gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca
     1561 caggaaggtt aatagtaatc tgattagctt tcccttgata gagctctgta ttgaaggttt
     1621 ggtatgtctt accatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct
     1681 cttcttgacc aagtgagaag gtttcacagt tttcttgccc accgaagtta tcaagtgata
     1741 accaagtctg tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt
     1801 cctgtcctag taatttcttg gtcaataagg catttatgga ctcacggaag tcaattgatt
     1861 gccaattgtt catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tgtatatgag
     1921 taggaagagc atggaacatc tggtaaacat gaagtggttt gacattccaa tcctgagaac
     1981 catgagtaaa gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat
     2041 gccaaaactg attgtagtcc ccagtttttc ggtctagttg agctttcact ttttctaagt
     2101 cagcttggtg agcttcattg ccacggatat agtcgctagc caagagatta cgagaatagg
     2161 ttaactcagc aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac
     2221 cgttttcacg gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta
     2281 aaccatcgac tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcccg
     2341 ttgtagcaac ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag
     2401 taaaggcacg gcaacggccg ttaagccaat cgatgacatt tttataagcc tcgatttgct
     2461 ggtagtctcc attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga
     2521 gattggcaaa gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag
     2581 ttagcttttc ctcagcctct gctataagct cagctttacc ttggggttgg acgagattta
     2641 gttgaggttt ctctagctca atcttgtgag gaagtttaac ctcaagctcg ccctccatct
     2701 tgtagagagc cttgtcacta gccttgtcat tggttccctg atgataaggg ctggctgtca
     2761 tgatggcagg gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg
     2821 atagcccttt ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat
     2881 cctggttaga aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg
     2941 gaataagacc atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat
     3001 tgagtaactg atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt
     3061 tacgaaaaac gtcactatct tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag
     3121 caacagtata aaaaatatct gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt
     3181 ctgttttttt atcagctgct aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa
     3241 agaaacgacg taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa
     3301 agccacattt ttttagttca gataagacat tttcttttgg aaaattgata taactatatt
     3361 gattaaaacg catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa
     3421 agcagaaagg gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact
     3481 aaattcaata tagagaacag agtaacaata agaataaata gatagggtat aaaagttcta
     3541 ggagatttat attatatgct ttctattttt atatacaata tagtataaat ataaaaatga
     3601 tgacaaaaat acaaatgaat agaaaataaa ttagtaagct gatgaaattt ttctcaagag
     3661 aagccattta taggtgaaaa tggtataata tagtgagaag gatagaggag aagtgtaaat
     3721 tgatcgcaca actagataca aaaacagtct atagttttat ggaaagcgtc atttcgatcg
     3781 aaaagtatgt gagagcagct aaagaatacg gctacactca tttggctatg atggatattg
     3841 acaatcttta tggcgctttc gactttctag agattacaaa aaaatacggc attcatcctt
     3901 tgctagggct tgaaatgaca gtgtttgtag atgatcaggg agtgaatttg cgctttttag
     3961 ctctatctag tgtgggctat cagcagttga tgaagctttc gacagccaag atgcaggggg
     4021 agaaaacttg gtcagtcctg tcccagtacc tggaggatat cgcggtcatt gtgccttatt
     4081 ttgatagagt tgagtcgtta gaactaggct gtgattacta tataggggtt tatccagaaa
     4141 cactagcaag cgaatttcat catcctatct tacctcttta tcgggtcaac gcttttgaaa
     4201 gcagggatag agaagttctt caagttttaa cagcgattaa agaaaatcta ccgctcagag
     4261 aagttccctt gcgttcgaga caagatgtct ttatatcagc aagttcttta gagaaactat
     4321 tccaagagcg ttttccgcaa gctttggaca atttagaaaa gcttatttca ggcatttctt
     4381 acgacttgga tactagtctg aaactgcctc gttttaatcc agctagacca gcagtagagg
     4441 agttgagaga gcgtgctgaa ctggggcttg ttcagaaggg gttgactagt aaagaatatc
     4501 aagatagact agaccaagaa ttgtctgtta ttcatgatat gggctttgat gattatttct
     4561 tggttgtttg ggatttgttg cgttttggac gatcgaatgg ctattatatg ggaatgggaa
     4621 ggggttctgc agtaggcagt ttggtttctt atgccttaga catcacgggg attgacccag
     4681 tagagaaaaa tctgattttt gaacgctttc ttaatcgtga acgctatacc atgcctgata
     4741 ttgatattga tatcccagat atttatcgtc cagattttat cagatatgtt ggtaataaat
     4801 atggtagtaa acatgcggca caaatcgtta ctttttcaac ctttggagcc aagcaagctc
     4861 ttcgagatgt cttgaaacgc tttggtgtgc cagagtatga attatctgca attactaaga
     4921 aaatcagttt tcgtgacaat cttaagtcgg cctatgaggg caatctccag tttcgtcagc
     4981 aaatcaatag taagttagaa taccaaaaag cttttgagat tgcttgcaag atagagggct
     5041 atccaaggca aacctctgtc catgcggctg gtgttgtaat tagtgaccaa gatttaacca
     5101 actacattcc tctaaagtat ggtgatgaaa ttccactgac tcagtatgat gctcatggag
     5161 ttgaggctag cggacttttg aagatggact ttctgggact acgaaatttg acctttgtcc
     5221 agaagatgca agagttgctt gctgaaacag aaggtattca tctgaaaatt gaagaaatcg
     5281 atttagaaga caaagaaaca ttagctttat ttgcctctgg taatacaaaa ggtatctttc
     5341 aatttgagca accaggtgcc attcgtctgc ttaagcgtgt gcaaccagtc tgttttgaag
     5401 atgtcgtcgc gactacttct ctaaatcgac cgggtgctag tgactatatc aataattttg
     5461 tggcaagaaa gcatgggcag gaagaagtga ctgttctgga tccagtactg gaggatattt
     5521 tggctccaac ctacggcata atgctctatc aggagcaggt tatgcaggtt gcccagcgac
     5581 ttgccggatt tagtcttggg aaagccgata ttttgcgtcg ggctatgggg aaaaaggatg
     5641 cctctgccat gcatgagatg agggcttcct ttattcaagg ttcattagaa gctggtcata
     5701 ctgtggaaaa agcagagcag gtctttgatg ttatggagaa gtttgcaggt tatggtttta
     5761 acaggtcaca cgcctatgcc tactcagcct tggccttcca gttggcttat ttcaaaacgc
     5821 attatccagc cattttttat caggtcatgt taaattcttc caacagtgat tacttaatag
     5881 atgcacttga agcaggtttt gaagtagcct ctctatccat caacaccatt ccctatcacg
     5941 ataaaattgc caacaaggcc atctatctag gtttgaaatc cattaaagga gtcagtaatg
     6001 atttagctct ctggattatt gaaaatagac cttattctaa cattgaagat tttatagcta
     6061 aattacctga gaattatctg aaacttcctc tgctagaacc tttggtaaaa gttggtcttt
     6121 tcgattcatt tgaaaaaaat cgtcaaaaag tatttaataa cttagctaat ctatttgaat
     6181 ttgtgaaaga gttgggaagt ttgtttggag atgctattta tagttggcag gaatcggaag
     6241 attggacgga acaagaaaaa ttttatatgg aacaagagct tttagggata ggtgtcagca
     6301 aacatccact acaagctatt gcaagtaagg ctatttaccc gattacccca atcggaaatt
     6361 tgtcagaaaa tagctatgct attatcttgg ttgaagttca gaaaataaaa gtgattcgta
     6421 ccaaaaaggg tgaaaatatg gccttcttac aggcagatga tagtaagaaa aaattggatg
     6481 ttacactctt ctcagattta tatcgacagg ttggtcagga agtaagagaa ggagcattct
     6541 actatatcaa agggaaagtc caatcacgtg acggtcgtct gcaaatgatt gcacaagaaa
     6601 taagagaagc agttgctgaa cgcttttgga tacaggtgaa aaatcatgaa tcggatcaag
     6661 aaatttcacg tattttagaa caatttaaag gcccaatccc agtcatcatc cggtatgaag
     6721 aggaacagaa aaccatcgtt tctccccatc attttgtagc taaatctgat gaattagagg
     6781 cgaaattgaa tgaaatcgtt atgaaaacga tttatcgcta aaaatacgga aaatagaaga
     6841 attttaaaat caaatgtgat ataatcaaga agaatgttaa aagaaaaagg agcataacca
     6901 atatgaaacg tattgctgtt ttgactagtg gtggagacgc ccctggtatg aacgctgcca
     6961 tccgtgcagt tgttcgtcaa gcaatttcag aaggaatgga agtgtttggt atctatgacg
     7021 gatatgctgg tatggttgcc ggtgaaattc atcccctaga tgcagcttca gtaggggaca
     7081 tcatttctcg tggtggtact ttccttcact cagctcgtta cccagagttc gctcaacttg
     7141 aagggcaact taaagggatt gagcaattga aaaaacacgg aattgaaggt gtagttgtta
     7201 tcggtggtga cggatcttac cacggcgcta tgcgtttgac tgaacatggc ttcccagcta
     7261 ttggtcttcc aggtacaatc gataacgata tcgttggtac tgactttaca atcggttttg
     7321 acacagcggt tactactgcc atggacgcta tcgataagat tcgtgataca tcatcaagtc
     7381 accgtcgtac ttttgtaatc gaagttatgg gacgtaacgc tggtgatatc gctctttggg
     7441 ctggtattgc aactggtgct gatgaaatca tcatccctga agcaggcttc aagatggaag
     7501 atatcgtagc aagcatcaaa gctggttatg aatgtggtaa aaaacacaat atcatcgtct
     7561 tagctgaagg tgtgatgtca gcggctgaat ttggtcaaaa acttaaagaa gctggagata
     7621 caagcgacct tcgtgtaaca gaacttggac atattcaacg tggtggttct ccaactgcgc
     7681 gtgaccgtgt tttggcgtca cgtatgggtg cacatgctgt taaacttctt aaagaaggta
     7741 tcggtggtgt tgcggttggt attcgtaacg aaaaaatggt tgaaaatcca attcttggta
     7801 ctgcagaaga aggggcattg tttagcctta ctgcagaagg taagattgtg gttaacaacc
     7861 cgcacaaagc cgatattgag ctatctagct tgaataagag cttgtcataa tttacaattt
     7921 agttaataag accaaaaagg tcatatataa aggagtcaca aaaatcatga acaaacgtgt
     7981 aaagatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa aattcggtga
     8041 ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca ttgctaaatt
     8101 gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc accaagaaca
     8161 aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa aagttggttt
     8221 ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg aagctaaaga
     8281 atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa tcaaatcaac
     8341 tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg atgttgaagt
     8401 tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg ctaaagatga
     8461 tgcaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta aacaaaaagg
     8521 tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg ataacgacga
     8581 tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg tacgtactgc
     8641 aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac atgttcaatt
     8701 attcgctaaa atcgaaaacc aacaaggtat cgataactta gacgaaatca tcgaagcagc
     8761 tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg aaatggttcc
     8821 agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg ttatcactgc
     8881 aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag aagtatcaga
     8941 cgtattcaac gctgttatcg acggaactga cgctacaatg ctgtcaggcg agtctgcaaa
     9001 cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga atgctcaagc
     9061 tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact ctaagacaga
     9121 agtaatggct tctgctgtta aagatgctac tagctcaatg gatatcaaat tggttgtaac
     9181 tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa atgctgacat
     9241 cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact ggggtgttat
     9301 cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg ctgaacgtaa
     9361 agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg ctggtgtgcc
     9421 agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt aagaaaaata
     9481 taaaaaccta tcatatccag ctttagagct tgtgtgatag gctttttgta tagagggtaa
     9541 gaaataggca aaactttcat aatggattga tactcttcga aaatcaaatt caaaccacgt
     9601 cagcgtcgcc ttaccgtata tatgttactg acttcgtcag ttctatctac aacctcaaag
     9661 cagtgctttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
     9721 gaaataagat atgcacaaat tgattaggaa attcaaatga atttctacaa atgttttagc
     9781 aatcgtaatg tacttgtcta gattcgatct gatatatttt cgatttaatg atatggtatt
     9841 taaaacctcc aaagtagctt gctccattct tttacttacg tgagtgtaga tgttatttac
     9901 tgttttagcg tttttgtgtt ccactctaac cattatagca ttcttctcag ctagtgtact
     9961 aaggagtgtg tgcctgaaaa tatgggaact aaggggctgg tttatcggtt tctctagttt
    10021 agtatttgcc ttttgcaaag tgatgttaaa tgcctttctc taaatttaca tatcactatt
    10081 gtttaacaaa atctaatcta ctttaggtca cttattcttt ttttgaaatg tagaatgaac
    10141 tttttcaaag tttttcgaat cttttaaaat ctgtttgttt tatatcgcca ttctccccct
    10201 tttttaattc tccctatata gcctgacaac tttcccgatg gtacgaatat ggttgctttc
    10261 gtctaggtgg atgtcggggt atttgggatt gagttttttt aaggtagcct tggcggagtt
    10321 tcttgacata gttagcgccg tctacttgga agatgccgat ggtattatag tcaatctgtg
    10381 gggtattctt gataaatagg tagtcgctgt ttcttatctt tggctccatg gacttgctga
    10441 cgacataagc gattgggtcg tagtcgtctg ggataatgga aactccataa ctaaatcgtc
    10501 gtcctgcatc gagcggctac ctgcagagat aaactaccta acacgagagt aagtagtctg
    10561 tctgtagtcg tccagtctga tgatttttac gatatttcgt ttttctgctc atacagttgc
    10621 ctctcggcat aggtcagaac tttaccttgt ctgggtggtg cccgttggtc gtagatagat
    10681 tggatatcgc taggagaatc cttttgaact ggaggaaaga gggtatcaat caagctactg
    10741 aatactttag ctaagtcaaa tatagtattt ttcttagtag acctaaccct tttttcataa
    10801 tttccaatgg tgtttttact tataccaatc ttagtaccca attcttattg agtccaacca
    10861 ttactagtct atattgtttt atagttgatt gagtttggaa tagtacgctg tagctgctaa
    10921 aacatttcta ggaattaatt tgactttcct aatagagttg ttcatatctt atttcaatct
    10981 actatgtttt tcacctctaa caatcgcaat ctcttcttta tccatgaatg aaatcgcttt
    11041 ctatttttgt aagtaaagca tcacacgaaa tccacgaaaa tgaaaacctt tgttgtgttt
    11101 tcgtaaaaaa tttgttgaca gagcacgaaa cgcctactat aattaaatca agcttaagga
    11161 aataacaaaa aaacaaatcg gagggaaaga cgatgaacac attaaatgag aaagtaatca
    11221 atatctgtaa agcagtagtt aaagaaactt taatccaaga catttaggag aaagaaatga
    11281 agaaatcaag aaaattagcc acattaggaa tctgttcagc cttgttttta ggcttggctg
    11341 cttgtcaaca acaacatgct acttctgagg ggacgaatca aaggcaaagc agttcagcga
    11401 aagttccatg gaaagcttca tacaccaacc taaacaacca ggtaagtaca gaagaggtca
    11461 aatctctctt atcagctcac ttggatccaa atagtgttga tgcatttttt aatctcgtta
    11521 atgactataa taccattgtc ggctcaactg gcttatcagg agatttcact tcctttactc
    11581 acaccgaata cgatgttgag aaaatcagtc atctctggaa tcaaaagaag ggcgatttcg
    11641 ttgggaccaa ctgccgtatc aatagttatt gtcttttgaa aaattcagtc accattccaa
    11701 agcttgaaaa gaatgaccaa ttgcttttcc tagataacga tgcgattgat aaaggaaagg
    11761 tctttgattc acaagataag gaagagtttg atattctatt ttcgagagtt ccaactgagg
    11821 caactacaga tgtcaaggtt cacgctgaaa agatggaaac attcttctca caatttcaat
    11881 tcaatgaaaa agctcgaatg ctgtctgtag tcttgcacga caatctggat ggcgagtatc
    11941 tgtttgtagg ccatgttggg gtcttagtac ctgctgatga cggtttctta tttgtagaga
    12001 aattgacttt cgaagagccc taccaagcga ttaaatttgc tagtaaggaa gattgctaca
    12061 agtatttggg caccaagtat gcggattata caggcgaggg actggctaag ccttttatca
    12121 tggataatga taagtgggtt aaactttaat agtgttatga tatgacagta atcttaggat
    12181 ttttatttct atttttgatg acttggtggg ttatttcaat cgttaatagc cagaaaccac
    12241 gcaaaaatga aacatttatt ggctatatcc aaagatacga tattgatggg aatggctatc
    12301 aatcattacc gtgtcctcag aactgagagg agttcttgaa accgtaaaac cgctttgaac
    12361 aagagttact tcaacccatt ggctccgacg gagtaagttg ctttcgtgaa taccaaaatc
    12421 agccgcaatt t
//
LOCUS       DAVBCR010000059        12136 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCR010000059 DAVBCR010000000
VERSION     DAVBCR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.059"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            119..1381
                     /locus_tag="VQ508_001563"
     CDS             119..1381
                     /locus_tag="VQ508_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2490535.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(1674..2144)
                     /gene="nrdI"
                     /locus_tag="VQ508_001564"
     CDS             complement(1674..2144)
                     /gene="nrdI"
                     /locus_tag="VQ508_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2490536.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(2222..2755)
                     /locus_tag="VQ508_001565"
     CDS             complement(2222..2755)
                     /locus_tag="VQ508_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490537.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            complement(2816..4102)
                     /locus_tag="VQ508_001566"
     CDS             complement(2816..4102)
                     /locus_tag="VQ508_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490538.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(4114..5760)
                     /locus_tag="VQ508_001567"
     CDS             complement(4114..5760)
                     /locus_tag="VQ508_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2490539.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(5762..6376)
                     /locus_tag="VQ508_001568"
     CDS             complement(5762..6376)
                     /locus_tag="VQ508_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2490540.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            6511..7080
                     /locus_tag="VQ508_001569"
     CDS             6511..7080
                     /locus_tag="VQ508_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2490541.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(7110..7802)
                     /locus_tag="VQ508_001570"
     CDS             complement(7110..7802)
                     /locus_tag="VQ508_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490542.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(7804..8865)
                     /locus_tag="VQ508_001571"
     CDS             complement(7804..8865)
                     /locus_tag="VQ508_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2490543.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            complement(8858..10231)
                     /locus_tag="VQ508_001572"
     CDS             complement(8858..10231)
                     /locus_tag="VQ508_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2490544.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(10331..11185)
                     /locus_tag="VQ508_001573"
     CDS             complement(10331..11185)
                     /locus_tag="VQ508_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2490545.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            complement(11339..12112)
                     /locus_tag="VQ508_001574"
     CDS             complement(11339..12112)
                     /locus_tag="VQ508_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2490546.1"
                     /translation="MLAACSGGAKKEGQAASKKEIIVATNGSPKPFIYEENGELTGYE
                     IEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPI
                     AQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKA
                     DFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQD
                     ELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK"
BASE COUNT         3462 a         2778 c         2252 g         3644 t
ORIGIN      
        1 attttctttg taaattttct atcttttttc aaaatattct tgcatttttc caaaattttt
       61 tgtagaatat agattacata tacagattat tctgaaaatt caaaaaggag tatttatcat
      121 gtcgcaagct atttccttaa atcaatcaac ctgggcaagc aagctaaaag caatgggacc
      181 tggaatccta atggcaactg ccgctgttgg aggttcccac attgtatcct caactcaagc
      241 tggcggttct tacggttggt ctctacttct cttggtcatc ttagccaatg tctttaaata
      301 tccatttttc cgttttggtg ctgaatacac agctgatact ggaaagactt tggttgaagg
      361 ttatgccgaa aaaggaaaac tctatctctg gattttcttt atcctcaatg tcttttcggc
      421 tatggtcaac acggctggtg ttgccattct gtgctcagct atcatcgcca gtgccttccc
      481 aatgattgga cttagcatta ctcagtggtc cctcattctc gttgcaatca tttgggctat
      541 gctactcttt ggaggctaca aacttttaga cggcatggtc aaatggatta tgtctgcctt
      601 aaccattgcg actgttctgg cagttatcat tgcggcggtc aagcatccag aatacagttc
      661 tgattttgtc gagaagacac cttggcaaat ggcagctctg cccttcatcg tctccctcct
      721 aggatggatg ccggctccta ttgaaatttc agccatcaat tcactttggt cagctgaaaa
      781 gagaaagacc gtcaacttta acacagaaga cgctctgttt gactttaaca ctggttatat
      841 tggaacagct atcctagccg tcttctttgt ggcactggga gcactgattc agtatcctac
      901 agggcaggcg gttgaagctg cttcagccaa atacatctct caattcgtgg gcatgtatgc
      961 ctctgttctt ggcgaatggt cccgttactt gattaccttt attgccttcc tctgtatctt
     1021 tggaacagtt ataactgtta tcgatggcta ttctcgcgtt aatcaggaat ctctccgact
     1081 gctaatcagt caaaaagagg acaatcgtaa atctttgaac atctggatga ccatcactgc
     1141 tatcatcggt atcgtcatta tcaagttctt ctctggtcag gtttcaacca tgctccgctt
     1201 tgccatgatt ggctctttcc tgacaacacc tttctttgct cttttgaatt acgccttggt
     1261 aacgcgtgaa aacaaaaatc ttccttcttg gctcaaacac cttgccattg cgggattgat
     1321 tttcctcttt ggcttcgcca tcttctttat ctacgcactc gcaatcggaa aagcagggta
     1381 agggacaagc gcgagatgaa gataaggttt catttcaaga gaaaattcag caaatatttc
     1441 tatgataaaa agcataagaa caaggttttg aagacctgaa cttatgcttt tttacgttct
     1501 taaagactgt ttatactcaa aaaacagttg aacaacttca accacctctt ataagaattt
     1561 tatactattc gagaatctct tcaaaccacg tcagctctat ctgcaacctc aaagctgtgc
     1621 tttgagcaac ctgcgactag cttcctagtt tgctctttga ttttcattga gtattaattc
     1681 tccttttcca actcatacaa atctgcgata atagctgcga catgtttgat atcttccagc
     1741 atgcctcgca tttcaaagtc agccaataca ggaaagccaa agcgttgact gtattgcttg
     1801 gctgttaggc agtattggtt attaaagtta cgatttcctg acccaaccac accaaaacac
     1861 ttactagcat tgttaccata ggcaataaaa tctcccaccg gtgtcgtcaa aatctcaaca
     1921 tctccgttat ccacgccatt cccaccttcg agataggtcg gcaaaaaagc gacataggga
     1981 tggtccattt catagaaatt tttgccttcc ttgactaaat ccttgatatg aatcttttga
     2041 acctcaatcc ctttgtactg ggacaagaga tagtctttca agcgcgtcac aaaactttca
     2101 gtgttgccac tcaaactaat ataaactaaa gaaattatct tcatatgtac ctccatgatt
     2161 ttatctataa tcaaaaaaca actaccaaga ttgtatcttg atagttcttt tttgtcaata
     2221 cttaggcaag ctcttctgtt tcctcttctt ctgccgaggc tttctgttgt cgccacatct
     2281 tatagaagac aagggctagg aagagaacat tgatgacgat atagaaaatg ctaacagctt
     2341 ttgaaccaat actcactgtc aaacgcatgc tctcatcttg aaccaactgc acagcgtctt
     2401 gtaaaccaac atagccataa atcgaaccaa taatagctaa caaaacatag ccaacgtaag
     2461 tatagtttgc atattgcaaa tttttacgaa caaggaagac aatcgctacg accactaaaa
     2521 tagtagatag cacaatcaaa gcaacattaa aaatagaatg agatgcttct gctactctat
     2581 aggtataatt aagctggtct tctaattgct gagcattgac ccctgcaact tgtttctgag
     2641 cagtacgaag ggcttcttta ctaggcaagg gactcacaat tccaaacaga gacggaactg
     2701 aaataagggc agacaagatt agcaagaccc atagataaat tggtttcttt ttcatgatag
     2761 aacctcctaa taataatatt tatattatag cacattttgt aaaggaatac aaatcttata
     2821 gtatagtttc tacctgtttt cggtaggctt ttggcgattt tccgcacaat tttcggaaaa
     2881 ctttatagaa atatccaagg ttactgtaac caacagcata acaaatatct tcgatgctgt
     2941 cactagttga aagcaaaagt tgttgggcag ccttaatacg ttgtttgttt agtaactctg
     3001 caaaagtcga atcggtttca cgcttaatca actgccctag atagacagga ttgataaaga
     3061 gagccttact gatatccttg agggaaagct ctttttggta atcacaacca atgacttcca
     3121 gcacactgac cacattttca ttcatacggt attgaccgaa aaagctgctc agagtttcct
     3181 tgatataagg aaccaattca tcgaaggatt gaatagcatg aatggttttg acgatgtctg
     3241 tcaggtcatc agctttgaga tgttcaaata aatggaaaac atccatgaca aactgaataa
     3301 aaagctgttt agtaatcgga acacgcggcg tattttcaag aactaccttc tccaagagat
     3361 ttaactcttc tacgatttga ttgagatttc cctgaatgat aaccctataa atcggttcgt
     3421 aataggcaaa gagactctca gactgctgca aatttacaga accgtaaaag agggttttct
     3481 cagcattcag cttccagcgt tcaaagtgtt cttgataagg agcttcaaaa ggtgtaacga
     3541 ctaaaccatc taggggttgg tcagaaatga aaatctgcca gtcttggccc aagacatagt
     3601 agggaatggt gaaggaacct tgtttttcct tggatagacc tatccaccaa ttctccttat
     3661 cccctaaata actaacaaat ccagcctcgt ctaattcttg actaagagtc tgacttttct
     3721 tccctctctc gccgagctga cctgcaatct tctccagcag atttcccagc tctaccttat
     3781 caacaggctt gaccaaatag tccaccacac taaggttcat tgctcttttt acataatcaa
     3841 actcctgata acctgagagc aggatatagg cagcatctgg taagatctct ttcatctccc
     3901 gaatcatatc aagccctgtt ttgtctggca tattgacatc ggaaatgatg acatcgacag
     3961 gattttcctg aacatattct agagcttcat cggcatgact ggctgttgcg acgacctcca
     4021 tatcccactt atcaaaggga atcaaacgct tcagaccttc tgtcaccatg tactcatcat
     4081 ctactaataa tactttatac attttctccc tttctactca tcttgaattg taatacgata
     4141 ctgaacaccg gcttgctctg cagactctat cgtaatggca tagcggtctc caaaatagag
     4201 cacaaaacgc tcgtgtacat tgacaatccc gatagactgc ctttgatcac tgtagctggc
     4261 tcggtgttca aaatatttct gacttaattt ttctcggata tttgccaact tttcagccga
     4321 cattcctcta ccattatcga ccaccaaaat ttccacaaaa ccatcctgtt taagagcctt
     4381 gatgctaatc acattatctg tccgcctgtg gtcaacacca tgcgcgaaat agttttctac
     4441 cagcggttgc aaggtaaact tgggaatctt catattctct aactctggat ctatcttgaa
     4501 accataggca atggacttgg gatagcgaac catgcagaga tagctgtatt tacggcaaaa
     4561 ttctaattcc tgtttgagga gggtctctct ttcgtcggaa atattgttac gcaagagact
     4621 actgaattca taaatgatat ctgccaactc atcttgactc tgcataactg catacatgcg
     4681 caagaactcc agcgtattat acataaaatg aggattgatt tgcgcctgca aggctcgcat
     4741 attggcatct ttttgactaa gctctaactg gtaaatatca tggatattct tttccaatcg
     4801 atccaacata tcattggtcg tctccgcgat taggagtaat tcctgatctt tttcggaaat
     4861 gtcaatccga cgcccctctt cgccttgagc aatgacttga atggattcca ctaaatccac
     4921 tacctgcttt tggtaattag caaaagtctg cctcaaagtc agataaagaa tgacaataaa
     4981 gagaaggctc aaacccacaa tcaaagcaga gctagtcact gttccttgta aaacaaagtt
     5041 tttaggaact gccacctgaa cctgatatcc atgagaagtt aagccaacta gccaattttc
     5101 actctcccta tcaactgtct caccaatatg aaaaatctcc gtatcaaaag gtgaggtcac
     5161 tgttactgcc atcggagtat gacctctagt attatcaatg gcatggtata aaacagcagg
     5221 atccaaggag atgtaaatca ctcctaaatc ttgatctgac actggatctg acaccggaat
     5281 ggcaaaacta tttcctgctg gtttgaaatc ctcagccctg attttttctc cactacgttt
     5341 gtctttagta gaaacataga cttccttgta atcttgaaaa gcaatggcca ccccagttac
     5401 aaaatcattt cgaacataga ggtcatcaac attttcatac agagagactg atatataagg
     5461 cgaagctttc cgctctaatt gccagtaaaa atagtctggc atgctaaggc taaagtattt
     5521 atagattccc tcaatacgag cttgattttc aactaaagcc tgagctatct gggttgactc
     5581 tctatggtaa tattcaattt cactcactgt tctagcagtt acacgctggg ctactctctg
     5641 ggcttccttt tcacgtgaat cccagtcagc gtaagagagt aagattgcaa aactcgcaat
     5701 gataatgatc atcactgaac tataggttct tagaagcgta tgtagccaaa actgcttcat
     5761 tctattttgt cgccaatttt tcatggatga tttcatagac aggttccaac atatcactga
     5821 taaagaaatg ccacatccca attcctacaa aaaatagcag ggcaggcata tagtaaccaa
     5881 ttgctacaag tagcacagtc ccaaggagaa ccttggccac agctgctaga ctcataaaga
     5941 tgccaatcaa ggataatttg agactatttc gataggacaa ggcaaaataa acttgtaatt
     6001 ttaaagatac tgtataagcc aaaaagatca gggcaactac taggacattc aaaagggtcg
     6061 caatcaaatg aacaatagtc tgatgaggca attgcaccaa gagatacaaa ccataggtca
     6121 aaactagacc cacacctaaa aagctataga aaatcaggtt gcttgagaca aaattttgct
     6181 tgtaaagaga ccaagcctcc ttcaaactgt attcccgaaa gctataacca tgttctgcat
     6241 ataagctcat caaggtggca ctagccggcg ctagaccaag gataatccct cctgctaaag
     6301 ttaatagcca aaagaaggct gtcgcaatca tccctaaaaa gaaacgatta aagacaaagt
     6361 ctaaaaatct acccatgata ttcccctaaa aataactatg aaaccattat atcatatttc
     6421 aagcgttttc aaaaacttct agtatccatt tacaatccca ccaaaccatg gtaaaatgag
     6481 aattgtgaaa aaattatcag gagacaattc atgaagaaat ctatcttaac tacactgctt
     6541 tttgcagttc tttacttcct ctgcatgggg attggtgtcc ttttgggcaa tctctttgac
     6601 caaactggaa acatgtttta tgcgcctgcc tttactgccc ttgtcggcgg tagcgtctat
     6661 atgatcctag tcgcaaaagt tccgcgcttt ggagccatta ccactatcgg ccttgtcatt
     6721 gccctctttt tcttgggaac taaacacggt gctggttcct tccttcctgg aattatctgt
     6781 ggcctcctag cagatggagt agctcattta ggaaaataca aggacaaaac aaagaacttc
     6841 ctttctttca ttattttcgc ctttagtaca acaggaccaa tcttgcttat gtggattgcg
     6901 cccaaagcct atatggctac tcttctggca agaggaaaat cccaagaata tatcgaccgc
     6961 atcatggtcg ctccaaaccc tggaactgtc cttctattta tcgcaagtat tgtcatcgga
     7021 gccctagtgg gtgccttgat tggacaagcc ttgagtaaaa aatttgcaca gaaaatctga
     7081 tcagttaaaa agagccacgc ggctcttttt tatttatggc tcaatttctt agtcaagaaa
     7141 tctcccaaga attggattgc aaagataatc aaaatgataa tgatggttgc caagatggtc
     7201 acatcgtgat tgtagcggtt aaatccataa gcgatggcta cattaccgat accaccagct
     7261 ccaaccgcac cggccatagc tgtttcccca acaagggaaa tcaaggtcac agtcgtcaca
     7321 cggatcaaat ctggaagacc ttctgatagg taaacaccca cgatgtccca gaaagtcgct
     7381 ccgctcgctt gagccgcctc aatgacaccg ccatccaatt cagccaagac aacctgcacc
     7441 tgacgggcaa agaaggcaaa gactgcaaaa gaaagtggga caagggctgc atttggcccg
     7501 atacttgttt taacaatcaa gtgagaaagt ggtgacaaga ttgccaagag gatgataaag
     7561 ggaactgcac gaaaaattga ggtaattttg tctaaaatcc agaatacgac tttattctcc
     7621 aagacaccac ctggcgctgt caagacgaga aagagacctg ccactagccc caagaagcct
     7681 ccgataatga aggaaagaac tgtcatataa agagttaagt agatagccgt tccccagcct
     7741 gcctgaccag cccaacccat tttatagaca tttggtaaat aggtttgaat caatgattcc
     7801 atcttactgt cctcccttca atacttttag ttgtacacct gcttgacgaa tggcttcttg
     7861 ggcacctgcc aacgctgctt tttcacctga caagaccacc accaattctc caacaggagt
     7921 accatcgaga atttcgatat tcccatagag aatattagcc attacttggt aatgcttgta
     7981 caattcattc aaaagtggct cgtctgttga agctccagcg tacttgagtt gcaccaagag
     8041 actgttttca gacaagtgtt ccacgatttc ttgcttctcg attttgacca tggcttcgtc
     8101 aatacctgta gctgttgaga taaagtcttg agtcaaaggt tgtttaggat ttgagaagat
     8161 ttcaagcaca ctaccctctt caatcaaatg cccatcctgc ataactgcaa cacggttggc
     8221 aatgtctttg acaatctgca tttcatgcgt aatcaagaca acagtcaagc ctaatttttg
     8281 gttcaaatct tgcaacaagg ccaaaatctg cttggttgtc ttcggatcaa gggcagaagt
     8341 tgactcgtct gaaatcaaga tttttggatc attggccaag gcacgcgcaa ttgccacacg
     8401 ctgtttttgc cctccagata gttgtgaagg gtagttttca gcacgatctg ccaaaccaac
     8461 caagtccaac aacttagcta ctttagcctt cttttcttcc ttgctgagtc cagagtgttt
     8521 aagggcaaag gctacattct cctctgctgt cttttggctc atcaggttaa aatgctggaa
     8581 aatcatcccg atatcttgac gtttacgacg caactgctct gccgtcaagg tgaccttacc
     8641 atcaaaaatc acatcgtcgt caatggtaat tttccctgca gatggttttt gcaagaggtt
     8701 aatcacccgt acaagggttg atttccctgc tccagaatat ccaacgattc cgtagatatc
     8761 cccttcttgg atgtgaatgg tcacatcctt aaccgctgtg atggttctct tcttttggtg
     8821 aaaagtcaca tcgatctgat ctaacttgat aatatctcta ctcatagctt ctaatcagct
     8881 cctctactaa ttcgatatgg gtgtaataat cagcgattcg cacattttca tctccaccgt
     8941 ggtctcggct attggcattt cctagaccaa atgcaaccat tggtacctct agggcatcaa
     9001 agaccgtatg cataggtcct gtccccgctg tcgtcggcaa gactgaaacg ccctgtggat
     9061 agaatttctt ggccaactcg atcacattga gaatggctgg tgcgctcata tcgcttcgat
     9121 agctcatctc tcccaaggta tagtataatt ctaccttatc aaagccattt ttgtctagtt
     9181 gtttccgaat tttttccaga acatcatgcg gttctaggcc cggaaccaga cgaacctcta
     9241 gcttggcact ggcttctgca ggtaaaatag tcttaacacc ctgaccttga taaccagact
     9301 ggattccttc gatattaagc gctggctcga aaaagaaacg ttttagaaag gccatccgct
     9361 cctcctgtaa gagaggcaac tccaatccat aaatccgact aacttcctct gggtttcgtt
     9421 gaccataagt ttctagcaag gccatttctc gttcattggg ctcttgtact tcttcgtaca
     9481 agccttcaac caagatacgg ccatccgcag cacgaagaga ctgtaaggct tggaggagat
     9541 accaaggagc tgattccaca acaccaccat aactcgagtg gatatccaca tcagcgcttt
     9601 ttaccttggc atcaaaggtc acaatcccct tattgccacc agaaatttcc agctgttcca
     9661 aggcattttt ggtcccttgt tcccagacca acaaatccgc cccacggagt ttgtctgcat
     9721 gcttttccaa atacttatct aggtctgttg aagccgattc ctccgctccc tccatgataa
     9781 agctgatatt gacaggtaaa tcatcatggt gctgcatata ttttctcaaa gcactcaagc
     9841 gagctgtgat atgacccttg tcgtcatcaa ccccacgccc atacatgaag ccattgcgga
     9901 ccgaaagcgt aaaaggatcc tctgtccaga cctgatcccc atccgctggc acagtgtcat
     9961 agtggttata gaaaatcaag gtcttggcat ctggacgcga actcttgaaa tgtgccatga
    10021 caaagggcgc tgtatagctc tcatcaatct ccacttcagc tccaacacgc ttgaaaatct
    10081 cacccagata atttgcgact tccttgagtc caacctgctg ggcaaagact gatttcttag
    10141 aaatcaaggt acgcaaaacc tcaaaataat gttggactac atgatccttt tcaaattttt
    10201 caatctgttc ttgttcgcta ggaaaaacca tattctctct acctttctat gaacgaatag
    10261 tcttctttac aatctatact ctatacaaaa gcaagaggtg gaattttctc tcccacctcc
    10321 ctgtttctta ttaccaaact ggttgatcca aaccatctga tgattcttcg ataacttttt
    10381 tcacgtcatc tgtgtggtaa gctgcgatta ctttcttgat agcatcagcc ttaggtgatg
    10441 tttcccaatc ttttttcgca acaatgatgt tgtaccattg ttttgagttt tcatcagctt
    10501 gttctttgaa aagtgctttc ttgtagtcca attttgcttc tgtaacgaag gtattgttta
    10561 caacggcagc gtcaactgat gacaatgaac gagctgtttg gctagcgtcc aattcagtga
    10621 ttttcaagtt ctttggattt tctttgatgt tggcaactgt tgcaagagca gttccagaaa
    10681 catccaattt aatcaagcca gctgattgaa gcaagtaaag cgcacggctt tcgtttgtag
    10741 cgtcattcgg tacagcgatt tctccgtttg ctgggatgtc ttctacttta gtgtacttgt
    10801 tggcacttcc attcaaacct gagtaaaggc ggattggaga gatgtaagta tctgcaatcg
    10861 ctacaaggtc ttttccgttt tctttgttcc agttgttcaa gaagttatag tgttggaaag
    10921 cgttcaaatc tacttcgcca tcagcagttg ctttgtttgg ttgtgagtag tctgtgaact
    10981 ctgtaaattc caaggtaatt ccgtcttttt taaccaattc ttggattttg tcccaacgtt
    11041 tttcttcaga accgctacgg ttaacagttg cgattttgat cgttgttgca ttgtctgctt
    11101 tcttttctga gtttccgcaa gctgcaagag ccaaacctgc gactgtagca agggctgcta
    11161 ggccaagcca ttttttgatt ttcatgattc tttctcctta aaaataatac cgtactagta
    11221 tctcacaatt tgtttgattt cacaaattgt tattagatag tcagatatat agtttaaaac
    11281 tatatcccta aaaactgaga aaccacccat cttactcaga atggatgatt tcaagaaatt
    11341 atttaatatc agcttctgcc ggtagataag tgtctccgaa gaattgttta gacaattttt
    11401 caagcgttcc gtctttgtag agttctttaa tgcgtttgtc tacaaatgat ttcaactcat
    11461 cttgaccctg agcaagaagt gggtaaacgt acggttgttg gtcgcttgga agttcgataa
    11521 ctttcaagtt gtccaaacct tggttcttga tcactgtttc aacaccgatt ttatcaaaaa
    11581 tcttgtagtc aaattgtcca tcgctcaaac gtaccatgat ttgttggaaa tctgccttag
    11641 tatagttaag gatagttggg ttgtccgtgt gttcagcgtt gtatgcttct aactgcttag
    11701 ctgatgtagt ggcttgaacg acttccgtcg attttccacc gatatcatcg agagacttga
    11761 tactagagtc atctttcttc actacaagga cattaggatt ttgggcaatt ggtgcggcat
    11821 agaggtattt ctccgcacgt tctttagtgt agctaagatt gttgacagcc atattgtaac
    11881 ggtcagcatc aagaccagca aagacacctg accattctgt cttttcaaac ttgacattat
    11941 atttgtcaga atctttaaag atagcgcgaa cgacttcaat ctcataacca gtcaattcgc
    12001 cattttcttc atagataaat ggctttggtg atccattggt tgcaacgatg atttctttct
    12061 tgctagctgc ttgtccttct ttcttagcac cacctgagca agccgcaagc acacctgcag
    12121 caacaagagc aagggc
//
LOCUS       DAVBCR010000060        11954 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCR010000060 DAVBCR010000000
VERSION     DAVBCR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.060"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(101..1732)
                     /locus_tag="VQ508_001575"
     CDS             complement(101..1732)
                     /locus_tag="VQ508_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2490547.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2025..7004
                     /locus_tag="VQ508_001576"
     CDS             2025..7004
                     /locus_tag="VQ508_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2490548.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLIALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7094..8290
                     /locus_tag="VQ508_001577"
     CDS             7094..8290
                     /locus_tag="VQ508_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2490549.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8426..8953
                     /locus_tag="VQ508_001578"
     CDS             8426..8953
                     /locus_tag="VQ508_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2490550.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            9030..9386
                     /gene="glnR"
                     /locus_tag="VQ508_001579"
     CDS             9030..9386
                     /gene="glnR"
                     /locus_tag="VQ508_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2490551.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRCALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9423..10769
                     /gene="glnA"
                     /locus_tag="VQ508_001580"
     CDS             9423..10769
                     /gene="glnA"
                     /locus_tag="VQ508_001580"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2490552.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10865..10984)
                     /locus_tag="VQ508_001581"
     CDS             complement(10865..10984)
                     /locus_tag="VQ508_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2490553.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11242..11379
                     /locus_tag="VQ508_001582"
     CDS             11242..11379
                     /locus_tag="VQ508_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490554.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            11472..11954
                     /locus_tag="VQ508_001583"
                     /pseudo
     CDS             11472..11954
                     /locus_tag="VQ508_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3841 a         2184 c         2565 g         3364 t
ORIGIN      
        1 tactatatat ctgcagaata ctctttcagt ttcattcagc ctagtcttgc ttaggaaaaa
       61 gtaaaaagcc atccaattat gatggatagc ttcttggttc ttagatttgt tctgcaaaaa
      121 ccttttcagc aaggttcata gcgtggtctg atacacgagt gtagtgtgag atgatgtcga
      181 taaagttgac cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg atgtgggttt
      241 ttctgagaac acgttctatc ttattgattg cttcatgacg ttctacaaga ctgcgtgctt
      301 tttcaatatc attgttttcc acactatcca gagcatcttt gataaagtca ctagtttggc
      361 ggtaaacttc ctctaattct ttcaaggcgg catcagaaaa ttcaacattt ttccgttgaa
      421 gatagtcagt cagattgagt agagcctccg tgtggtctcc aatccgttcc aaatcacggg
      481 aggaatcaag gatattggta agcacttcac tttctttttg gctgagagct tcgcttgaaa
      541 gggcaatgag ataacgtgtt aattgctcat cgatggtgtt aattgcttct tcggttttat
      601 gccctttttc agcaactttt tcatccaagt caatgatgta cttataggaa aggtcaaagg
      661 ctttagcagc gtagtttcct aagtgcaaga gctctttctt agcatttcct agagcgatag
      721 atggggcctg tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg acaacctcgt
      781 cctctccagg aataatcttg gttacaaagt aagccagagc tccgataaat ggaaattgga
      841 caatggtgtt ggtaatatta aaggttccgt gagcaaaggc gatggtcatt tccggtgcta
      901 gatttagcgt agcttcaaac caatgaatca ggacagtaaa aggaactaga aaaataacgc
      961 agacgactgt tccgataacg ttgaaggcaa catgagctcc tgctacccgt ttagctgcaa
     1021 tattagcccc taaagaggca atgatggctg taatggttat cccgatattg tcaccaaata
     1081 gaactggcaa agctccctgt aggtcaatta gattgccggc gtagaggttt tgtaaaatcc
     1141 caatggtagc cgaagaagct tgaattagca aggtcaagcc ggtaccgaca aagacaccca
     1201 aaacaggatt cttacttagc tcaatcatat agtccttaaa gacctgtaaa tccttgagtg
     1261 gagccattgc gccgctcatg agattgaggg caaaaaagat accaccgaca ccaaagagga
     1321 tgcgtccgat attattgact gtccgatttt ttgtaaaaaa gagacagacg gcaccgataa
     1381 agagcatagg tagggcatag ttacctaatt taaaaccgat gagaaaggat gtgacagttg
     1441 tcccaatatt agcacccatg acaatcccga tagcctgacg taaggttaag agaccggcac
     1501 tgaccaggcc gactgtgata actgttacac cagaactaga ctgaattaga gcagtcatcc
     1561 caataccaac cagaactcca aagaaaggat tactagtata tttgtcaatg taaaaacgaa
     1621 ggcgatctcc agcagcttgt tgcaaaccgt ctcccatggt cttgatgcta tataagaata
     1681 gccccagacc acctaaaaag tgaaataaaa tttcctgcca attaatggac atttcttttt
     1741 cctccgaaaa ataatcgcgg aatatcttct attctatttt aaaggataaa agtaaatcta
     1801 acaagtgtta atgtcaagtt tgtaaaagaa aattataatt gataagtgta ttatttttaa
     1861 gagtttttaa ccttatttta gatttgtgca aacagttatt gttttttgtc taaaaatgtt
     1921 ttaggataaa aatgtgatta tcatggttct agagtttgat aaaaaactac agaatataaa
     1981 gcgctttctt gaaaacaaca aaatcaatct tttaggagga gaaaatgaag aatccatttt
     2041 ttgaaagacg ttgtcgttac agtattcgta agttatcagt aggagcctgc tcgctgatga
     2101 ttggtgctgt tttatttgct ggtccagcct tggctgaaga aactgcagtt cctgaaaata
     2161 gcggagctaa tacagagctt gtttcaggag agagtgagca ttcgaccaat gaagctgata
     2221 agcagaatga aggggaacat gctagagaaa acaagctaga aaaggcagaa ggagtagcga
     2281 tagcatctga aactgcttcg ccagcaagca atgaagctgc aactactgaa actgcagaag
     2341 cagctagcgc agctaaacca gaggaaaaag caagtgaggt ggttgcagaa acaccatctg
     2401 cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa gcccgaagca actaaccaag
     2461 gggatgagtc taaaccagca gcagaagcta ataagactga aaaagaagtc cagccagatg
     2521 tccctaaaaa tacagaaaaa acattaaaac caaaggaaat caaatttaat tcatgggaag
     2581 aattgttaaa atgggaacca ggtgctcgtg aagatgatgc tattaaccgc ggatctgttg
     2641 tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa agctagcaag gaagcaaaag
     2701 ttcaagcctt atcaaacacc aattctaaag caaaagacca tgcttctgtt ggtggagaag
     2761 agttcaaggc ctatgctttt gactattggc aatatctaga ttcaatggtc ttctgggaag
     2821 gtctcgtacc aactcctgac gttattgatg caggtcaccg taacggggtt cctgtatacg
     2881 gtacactctt cttcaactgg tctaatagta ttgcagatca agaaagattt gctgaagctt
     2941 tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa attggtagac atggccaagt
     3001 attatggcta tgatggctat ttcatcaacc aagaaacaac tggagatttg gttaaacctc
     3061 ttggagaaaa gatgcgccag tttatgctct atagcaagga atatgctgct aaggtaaacc
     3121 atccaatcaa gtattcttgg tacgatgcca tgacctataa ctatggacgt taccatcaag
     3181 atggtttggg agaatacaac taccaattca tgcaaccaga aggagataag gttccggcag
     3241 ataacttctt tgctaacttt aactgggata aggctaaaaa tgattacact attgcaactg
     3301 ccaactggat tggtcgtaat ccttatgatg tatttgcagg tttggaattg caacagggtg
     3361 gttcctacaa gacaaaggtt aagtggaatg acattttaga cgaaaatggg aaattgcgcc
     3421 tttctcttgg tttatttgcc ccagatacca ttacaagttt aggaaaaact ggtgaagatt
     3481 atcataaaaa tgaagatatc ttctttacag gttatcaagg agaccctact ggccaaaaac
     3541 caggtgacaa agattggtat ggtattgcta acctagttgc ggaccgtacg ccagcggtag
     3601 gtaatacttt tactacttct tttaatacag gtcatggtaa aaaatggttc gtagatggta
     3661 aggtttctaa ggattctgag tggaattatc gttcagtatc aggtgttctt ccaacatggc
     3721 gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga atatgatttt acagatgcct
     3781 ataatggcgg aaattccctt aaattctctg gtgatgtagc cggtaagaca gatcaggatg
     3841 tgagacttta ttctactaag ttagaagtaa ctgagaagac caaacttcgt gttgcccaca
     3901 agggaggaaa aggttctaaa gtttatatgg cattctctac aactccagac tacaaattcg
     3961 atgatgcaga tgcatggaaa gagctaaccc tttctgacaa ctggacaaat gaagaatttg
     4021 atctcagctc actagcaggt aaaaccatct atgcagtcaa actatttttc gagcatgaag
     4081 gtgctgtaaa agattatcag ttcaacctcg gacaattaac tatctcggac aatcaccaag
     4141 agccacaatc gccgacaagc ttttctgtag tgaaacaatc tcttaaaaat gcccaagaag
     4201 cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga tttctatgaa gtttatgaaa
     4261 aagatggaga cagctggaaa ttactaactg gctcatcttc tacaactatt tatctaccaa
     4321 aagttagccg ctcagcaagt gctcagggta caactcaaga actgaaggtt gtagcagtcg
     4381 gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt tgattggggt atgactgtaa
     4441 aagataccag cctaccaaaa ccactagctg aaaatatcgt tccaggtgca acagttattg
     4501 atagtacttt ccctaagact gaaggtggag aaggtattga aggtatgttg aacggtacca
     4561 ttactagctt gtcagataaa tggtcttcag ctcagttgag tggtagtgtg gatattcgtt
     4621 tgaccaagcc acgtaccgtt gttagatggg tcatggatca tgcaggagct ggtggtgagt
     4681 ctgttaacga tggcttgatg aacactaaag actttgacct ttattataaa gatgcagatg
     4741 gtgagtggaa gctagctaag gaagtccgtg gtaacaaagc acacgtgaca gatatcactc
     4801 ttgataaacc aatcactgct caagactggc gcttgaatgt tgtcacttct gacaatggaa
     4861 ctccatggaa ggctattcgt atctataact ggaaaatgta tgaaaagctt gatactgaga
     4921 gtgtcaatat tccgatggcc aaggctgcag cccgttctct aggcaataac aaggtacaag
     4981 ttggctttgc agatgtaccg gctggagcaa ctattaccgt ttatgataat ccaaattctc
     5041 aaactccgct cgcaaccttg aagagcgaag ttggaggaga cctagcaagt gcaccattgg
     5101 atttgacaaa tcaatctggt cttctttatt atcgtaccca gttgccaggc aaggaaatta
     5161 gtaatgtcct agcagtttcc gttccaaaag atgacagaag aatcaagtca gtcagcctag
     5221 aaacaggacc taagaaaaca agctacgccg aaggggagga tttggacctt agaggtggtg
     5281 ttcttcgagt tcagtatgaa ggaggaactg aggacgaact cattcgccta actcacgcag
     5341 gtgtatcagt atcaggtttt gatacgcatc ataagggaga acagaatctt actctccaat
     5401 atttgggaca accagtaaat gctaatttgt cagtgactgt cactggccaa gatgaagcaa
     5461 gtccgaaaac tattttggga attgaagtaa gtcaggaacc gaaaaaagat tacctagttg
     5521 gtgatagctt agacttgtct gaaggacgct ttgcagtggc ttatagcaat gacaccatgg
     5581 aagaacattc ctttactgat gagggagttg aaatttctgg ttacgatgct caaaagactg
     5641 gtcgtcaaac cttgacgctt cgttaccaag gtcatgaagt caactttgat gttttggtat
     5701 ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa attagcagaa gttgaagctg
     5761 ctaagaacaa ggtggtctat aactttgctt catcagaagt aaaagaagcc ttcttgaaag
     5821 caattgaagc ggccgaacaa gtgttgaaag accatgaaac tagcacccaa gatcaagtca
     5881 atgaccgact taataaattg acagaagctc ataaagctct gaatggtcaa gagaaattta
     5941 cggaagaaaa gacagagctt gatcgcttaa caggtgaggt tcaagaactc ttggctgcca
     6001 aaccaaacca tccttcaggt tctgccctag ctccgcttct tgagaaaaac aaggccttgg
     6061 ttgaaaaagt agatttgagt ccagaagagc ttacaacagc gaaacagagt ctaaaagatc
     6121 tgattgcttt attgaaagaa gacaagccag cagtcttttc tgatagtaaa acaggtgttg
     6181 aagtacactt ctcaaataaa gagaagactg tcatcaaggg tttgaaagta gagcgtgttc
     6241 aagcaagtgc tgaagagaag aaatactttg ctggagaaga tgctcatgtc tttgaaatag
     6301 aaggtttgga tgaaaaaggt caagatgttg atctctctta tgcttctatt gtgaaaatcc
     6361 caattgaaaa agataagaaa gttaagaaag tatttttctt acctgaaggc aaagaggcag
     6421 tagaattggc ttttgaacaa acggatagtc atgttatctt tacagcacct cactttactc
     6481 attatgcctt tgtttatgaa tctgctgaaa aaccacaacc tgctaaacca gcaccacaaa
     6541 acacagtcct tccaaaacct acttatcaac cgacttctga tcaacaaaag gctcctaaat
     6601 tggaagttca agaggaaaag gttgcctttc atcgtcaaga gcatgaaaat actgagatgc
     6661 tagttgggga acaacgagtc atcatacagg gacgagatgg actgttaaga catgtctttg
     6721 aagttgatga aaacggtcag cgtcgtcttc gttcaacaga agtcatccaa gaagcgattc
     6781 cagaaattgt tgaaattgga acaaaagtaa aaacagtacc agcagtagta gctacacagg
     6841 aaaaaccagc tcaaaataca gcagttaaat cagaagaagc aagcaaacaa ttgccaaata
     6901 caggaacagc tgatgctaat gaagccctaa tagcaggctt agccagcctt ggtcttgcta
     6961 gtttagcctt gaccttgaga cggaaaagag aagataaaga ttaaatatcg aaaaatcttg
     7021 tgaaatcttt ccttatattt ccaaagtgtg atataatagt ttcgaataaa ataaataaag
     7081 gagtttttgt aacatggcaa aacttactgt taaagacgtt gacttgaaag gtaaaaaagt
     7141 cctcgttcgt gttgacttca acgtaccatt gaaagatggc gtaatcacta acgataaccg
     7201 tatcacagca gctcttccaa ctattaagta catcatcgaa caaggtggac gtgcaattct
     7261 tttctctcac cttggacgtg tgaaagaaga agctgataaa gctggtaaat cacttgctcc
     7321 tgtagcagct gacttggcag caaaacttgg tcaagatgtt gttttcccag gtgtcactcg
     7381 tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat ggacaagttc tcttggttga
     7441 aaacactcgt tacgaagatg ttgacggcaa gaaagaatct aaaaacgatc ctgaacttgg
     7501 taaatactgg gcatcacttg gagatggtat cttcgtaaac gatgcattcg gtacagctca
     7561 ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt gaaaaagcag ttgctggttt
     7621 ccttcttgaa aacgaaattg cctacatcca agaagcagtt gaaactccag aacgtccatt
     7681 cgtggctatc cttggtggtt caaaagtttc agacaagatc ggtgttatcg aaaacttgct
     7741 tgaaaaagct gataaagtcc ttatcggtgg tgggatgact tacacattct acaaagcaca
     7801 aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg cgaaagctct
     7861 tcttgaaaaa gcaaatggta aattgatctt gccagttgac tcaaaagaag ctaacgcatt
     7921 tgctggttac actgaagtgc gtgacactga aggtgaagca gtttctgaag gcttccttgg
     7981 tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa gctttgactg gtgccaaaac
     8041 agttgtatgg aacggaccta tgggtgtatt tgaaaaccca gatttccaag ctggtacaat
     8101 cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa tcaatcatcg gtggtggtga
     8161 ctcagctgcc gcagcgatta accttggccg tgcagacaag ttctcatgga ttagtacggg
     8221 tggtggagca tcaatggaac ttcttgaagg taaggttctt ccaggacttg cagccttgac
     8281 agaaaaataa gattttataa acaaatcaaa gaagagaggg atgaaagttc ctcttttctt
     8341 ttgcttaaaa taaaaacgct tcctctcaac tattactcat aaaaatcacc gatttatgat
     8401 aaaatggaaa tagaaagttg agattatgag ttattttaaa aaatataaat tcgataaatc
     8461 ccagttcaaa cttggtatgc gaacctttaa aacaggtatt gctgtttttc tagttctctt
     8521 gatttttggc ttttttggct ggaaaggtct tcaaattggt gctttgacag ccgtttttag
     8581 cctgagggag agttttgatg agagtgttca ttttgggact tcgcgtattc taggaaatag
     8641 tatcggtgga ctctatgcct tggtcttctt cttattaaat acctttttcc acgaagcctt
     8701 ttgggtgacc ttggtagttg ttccaatctg caccatgtta accattatga caaatgtagc
     8761 catgaataac aaagcagggg ttattggtgg tgtagcagct atgttaatca ttaccctatc
     8821 aattccaagt ggtgagacaa tttcgtacgt gtttgtgcgt gtatcagaaa cgtttatggg
     8881 agtttttgtc gcaattatcg taaattacga tattgatcgt gttcgtctct ttttagagaa
     8941 aaaagaaaaa taatgttaca ttttataaca ttatcaattg acgtttgtct ttttttagac
     9001 tataatagac agaaagaagg aaattgtaaa tgaaggaaaa agaatttcgc cgaaatatgg
     9061 ctgtttttcc tatcggcagt gttatgaagt tgaccgatct atcggcgcgt cagattcgtt
     9121 attatgaaga tcaagagttg atcaagcccg atcgaaacga aggaaatcgt cgcatgtatt
     9181 ccttgaatga catggatcgt ctgcttgaaa tcaaagatta tatctctgaa ggttataata
     9241 tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa atccaagaaa gcggttagtc
     9301 agactgaagt acgttgtgca cttcacaatg aactcctcca acaggggcgt tttgcttcag
     9361 tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag tcatagatta aggagaaaac
     9421 ttatgccaat cacagctgca gatattcgtc gtgaagtcaa ggaaaaaaat gttaccttta
     9481 ttcgtcttat gttctcagat attttgggaa ccatgaaaaa cgtcgaaatt cctgctacag
     9541 atgaacagtt agataaggtc ttgtcgaaca aggttatgtt tgatggatct tctattgaag
     9601 gttttgtacg tatcaatgag tcggatatgt acttgtaccc ggacttggat acatggacag
     9661 tcttcccttg gggagatgaa aatggaagtg ttgcaggtct gatctgtgat gtctatacaa
     9721 cagaaggtga accatttgcg ggtgaccctc gtggtaattt gaaacgagct cttcgtcaca
     9781 tggaagaagt tggattcaaa tccttcaacc ttggtccaga gccagaattc ttcctattta
     9841 agttggatga aaatggggac ccaacacttg aagtgaatga caagggtggc tactttgatt
     9901 tggcacctac tgaccttgcg gacaacacac gtcgtgagat tgtgaatgtc ttgaccaaaa
     9961 tgggatttga agtagaagcg agtcaccacg aggttgcggt tggacagcat gagattgact
    10021 ttaagtacga tgaagttctc cgtgcttgtg ataagattca aatctttaag cttgttgtta
    10081 aaaccattgc tcgcaaacac ggactttacg caacatttat ggcgaagcca aaatttggta
    10141 ttgctggatc aggtatgcac tgtaatatgt ccttgtttga tgcagaagga aataacgcct
    10201 tctttgatcc aaatgatcca aaaggaatgc agttgtcaga aacagcttac catttcctag
    10261 gcggtttgat caagcatgct tacaactata ctgccatcat gaacccaaca gttaactcat
    10321 acaaacgttt ggttccaggt tatgaagcgc ctgtttacat tgcttgggct ggtcgtaacc
    10381 gttcgccact tgtgcgcgta cctgcttcac gtggtatggg aactcgtctt gagttgcgtt
    10441 cagtggatcc aatggcgaac ccttacattg ctatggctgt tcttttggaa gttggtttgt
    10501 atggtattga aaataaaatc gaagcaccag ctcctatcga agaaaatatc tacatcatga
    10561 cagcagaaga gcgcaaggaa gctggtatta cagaccttcc atcaactctt cacaacgctt
    10621 tgaaagcttt gacagaagat gaagtggtta aagctgctct cggagatcac atctacacta
    10681 gcttccttga agccaaacga atcgaatggg caagttatgc aaccttcgtt tcacaatggg
    10741 aaattgataa ttatttagac ctttactaat actaatatag aagaaagatt gcctgtgggt
    10801 aaacagtcac acagcagtct aagccatcaa atacctataa cccttaaaat agcgatttcg
    10861 gtcgctattt atttttcgaa aaatcctctt gactcttttg cgaattagat ttatcatcag
    10921 acgtgtagaa gaaaatcaag gagtcactat ggaaaaattc aatttcaaaa acaatatcgg
    10981 gcaagaaaac aagttacttc aaatcgaaat ctacaagttt actaactttt gcaagcttca
    11041 aaattacaca tctgtaaata ttttttcgaa agatatattt gaagccatag taaattgaga
    11101 ctagaatagt acactacatc tgctaaaaat ttctagaaat taatttaact ttcctaatcg
    11161 atttgtttac atcttatttc aatctactat attatctcaa taaatgatga tggcgactgt
    11221 tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg aagaatattt ctgccgaggt
    11281 tgtcaagggg aaaatcctga agatattgaa ttttatgatg aacaattaca ggcagaaaag
    11341 gtggaggctt tgaatattcg attggaagta aaaaattgaa ttatctacaa tctcatcaac
    11401 aataaaccta tttcaacccc attatctata ggcgtatttt tacgcctttt tatatacttg
    11461 aagcacataa gaaacattat gggaagtttg ctgatggaag tactcaagaa attgatgttc
    11521 cttatgatat tcctgatact tgggagtggg tgaggataaa atcaatttat tggaattttg
    11581 ggcaaaataa gccagagaaa tcctttaggt atatagatac gtctagtatt gatagaaaaa
    11641 agaacataat caactacaaa aatctacaat atctttcacc tgaacaagcg ccttcccgtg
    11701 ccagaaaatt agtttcgcag aatagtgtct tattttcaac agttagacca tatctaaaaa
    11761 atattgctgt agttagagaa cttaaagagt atttgatagc tagtacagca tttattgttt
    11821 tggatacttt acttaacgaa acatatttga aatattattt attatcagat aattttatca
    11881 atcgtgttaa taataaatct actggaacaa gttatcctgc aatcaatgat tataatttta
    11941 atctattatt aatt
//
LOCUS       DAVBCR010000061        11835 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCR010000061 DAVBCR010000000
VERSION     DAVBCR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.061"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            173..2464
                     /gene="pcrA"
                     /locus_tag="VQ508_001584"
     CDS             173..2464
                     /gene="pcrA"
                     /locus_tag="VQ508_001584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2490555.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2507..3187
                     /gene="radC"
                     /locus_tag="VQ508_001585"
     CDS             2507..3187
                     /gene="radC"
                     /locus_tag="VQ508_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2490556.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3184..3873)
                     /locus_tag="VQ508_001586"
     CDS             complement(3184..3873)
                     /locus_tag="VQ508_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2490557.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3891..4532)
                     /locus_tag="VQ508_001587"
     CDS             complement(3891..4532)
                     /locus_tag="VQ508_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2490558.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4615..4830)
                     /locus_tag="VQ508_001588"
     CDS             complement(4615..4830)
                     /locus_tag="VQ508_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2490559.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4838..5185)
                     /locus_tag="VQ508_001589"
     CDS             complement(4838..5185)
                     /locus_tag="VQ508_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2490560.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5190..6305)
                     /locus_tag="VQ508_001590"
     CDS             complement(5190..6305)
                     /locus_tag="VQ508_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2490561.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6315..7274)
                     /locus_tag="VQ508_001591"
     CDS             complement(6315..7274)
                     /locus_tag="VQ508_001591"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2490562.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7387..7956)
                     /locus_tag="VQ508_001592"
     CDS             complement(7387..7956)
                     /locus_tag="VQ508_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2490563.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8084..8755
                     /locus_tag="VQ508_001593"
     CDS             8084..8755
                     /locus_tag="VQ508_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2490564.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8739..9557
                     /locus_tag="VQ508_001594"
     CDS             8739..9557
                     /locus_tag="VQ508_001594"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2490565.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9554..10450
                     /locus_tag="VQ508_001595"
     CDS             9554..10450
                     /locus_tag="VQ508_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2490566.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10494..11468
                     /gene="pta"
                     /locus_tag="VQ508_001596"
     CDS             10494..11468
                     /gene="pta"
                     /locus_tag="VQ508_001596"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2490567.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3440 a         2267 c         2550 g         3578 t
ORIGIN      
        1 aggtattgag ctagtttatg aaaaaattgg gtgaaaaagt cgagtgtttt agaaacccac
       61 agtgtagtat tctagtttca atccactata ttttgctact ccccgtaaag tttctatttt
      121 ccctgatttc tgatataata gaaatattga cttcaagagt aaggaagaga agatgaacgc
      181 attattaaat ggaatgaatg accgtcaggc tgaggcggtg caaacgacag aaggtccctt
      241 gctaatcatg gcaggagctg gttctggaaa gactcgtgtt ttaacccacc gtatcgctta
      301 tttgattgat gaaaagctgg tcaatccttg gaatatcttg gccattacct ttaccaacaa
      361 ggctgcgcgt gagatgaaag agcgtgctta tagcctcaat ccagctactc aggactgtct
      421 gattgcgacc ttccactcca tgtgtgtgcg tattttgcgt cgcgatgcgg accatattgg
      481 ctacaatcgt aattttacaa ttgtggatcc tggtgaacag cgaacgctca tgaaacgtat
      541 tctcaaacag ttgaacttgg atcctaaaaa atggaatgaa cgaactattt tggggaccat
      601 ttccaatgct aagaatgatt tgattgatga tgttgcttat gctgctcaag ctggcgatat
      661 gtatacgcaa attgtggccc agtgttatac aacctatcaa aaagaacttc gtcagtctga
      721 atccgttgac tttgatgatt tgattatgct gaccttgcgt ctctttgatc aaaatcctga
      781 tgttttgacc tactaccagc aaaaattcca atacatccac gttgatgagt accaagatac
      841 caaccacgct cagtaccaat tggtcaaact cttggcttcc cgttttaaaa atatctgtgt
      901 ggttggggat gcggaccagt ctatctacgg ttggcgtggt gctgatatgc agaatatctt
      961 ggactttgaa aaggattacc ccaaagctaa ggttgttttg ttggaggaaa attaccgctc
     1021 aaccaaaacc attctccaag cggccaacga ggttattaaa aataataaaa atcgccgtcc
     1081 taaaaatctc tggactcaaa acgctgatgg ggagcaaatc gtttactatc gtgctgatga
     1141 tgagctggat gaggctgtat ttgtagccag aaccatcgat gaacttagtc gcagtcaaaa
     1201 cttccttcat aaggattttg cagttctcta tcggactaat gcccagtccc gtacaattga
     1261 ggaagccctg ctcaagtcta acattcctta taccatggtt ggcggaacca aattctacag
     1321 ccgtaaggaa attcgcgata ttattgctta tctcaacctt attgctaatt tgagtgacaa
     1381 cattagtttt gagcgtatta tcaacgagcc taaacgtgga attggtctag gtacagttga
     1441 gaaaatccgt gattttgcaa atttgcaaaa tatgtctatg ctggatgctt ctgctaatat
     1501 tatgttgtct ggtatcaagg gtaaggcagc ccaatctatc tgggattttg ccaatatgat
     1561 gcttgatttg cgggagcagc tagaccactt aagcattaca gagttggttg agtccgtcct
     1621 agaaaaaaca ggttatgtcg atattcttaa cgcccaagcg actctagaaa gcaaggcaag
     1681 ggttgaaaat atcgaagagt ttctttctgt tacgaagaac tttgatgaca ccactgatgt
     1741 gacagaagag gaaactggtc tggacaaact gagtcgtttc ttaaatgact tggctttgat
     1801 tgccgacaca gattcaggta gtcaggagac atcagaagtg accttgatga ccctgcatgc
     1861 tgccaaaggt ctcgaatttc cagttgtctt tttgattggg atggaagaaa atgtctttcc
     1921 acttagtcgt gcgactgaag atccagatga attagaagaa gagcgccgtc tagcctatgt
     1981 aggtatcacg cgtgcagaga aaattctcta tctgaccaat gccaactcac gcttgctttt
     2041 tggtcgtacc aattataacc gtccgactcg ttttattaac gaaatcagtt cagacttgct
     2101 tgagtatcaa ggtctggctc gtcctgcaaa tacaagcttt aaggcatcat atagcagtgg
     2161 tagtatttcc tttggtcaag gtatgagttt ggctcaggct cttcaagacc gtaaacgcgg
     2221 tgctgcccca aaatcgatcc agtcaagcgg tcttccattt ggtcaattta cagctggcgc
     2281 aaaatcagca tctagcgagg caaattggtc cattggtgac attgctctcc acaagaaatg
     2341 gggagaggga actgttctgg aagtttcagg tagcggtgct aggcaggaat tgaaaatcaa
     2401 tttcccagaa gtaggtttga aaaaactttt agccagtgtg gctccaattg agaaaaaaat
     2461 ctaattttcc atccttctca cgaataataa agtgaggagg atttttatgt acagtatttc
     2521 attccaagaa gattcactat taccaagaga aaggctggcc aaggaaggag ttgaagcgct
     2581 tagtaaccaa gagttgctag ctattttact caggacagga acacgtcaag ctagcgtttt
     2641 tgaaattgcc caaaaagtct tgaacaatct ttcaagccta acggatttga aaaaaatgac
     2701 cctgcaggaa ttgcagagtt tgtctggtat tgggcgtgtt aaggccatag aattacaagc
     2761 tatgattgaa ctggggcatc gtattcacaa acacgagact cttgaaatgg aaagtattct
     2821 cagcagtcaa aagttggcca agaagatgca gcaggaatta ggggataaaa aacaagagca
     2881 cctggtggca ctctatctca atactcaaaa tcaaatcatc catcagcaga ccatttttat
     2941 cggttctgta actcgtagta tcgctgaacc gcgagagatt cttcactatg caatcaagca
     3001 tatggcgact tctcttatct tggtccacaa tcatccttca ggagcggtag cgcctagcca
     3061 aaatgatgat catgtcacta aacttgttaa agaagcctgc gaattgatgg ggattgttct
     3121 cttggaccat ttgattgtct ctcattctaa ttactttagt tatcgtgaaa agacagattt
     3181 aatctaaagt tcattaacga catagtcaaa gagtttttta tctttgggac gattttcaaa
     3241 aagaagttct ggatgccatt ggacaccgag aaaggcgaca tcatccgtac tcatgacagc
     3301 ctcaatgata ccatctttag gatcatgagc cacaactttt aaatttggtg ctaagtcctt
     3361 gatgctctgg tggtggaagg agttgatatg agagatttct ccatagattt cttggagaac
     3421 ggtatctggt tctgttacca agcgttgagt tgtgtactca acagaagaat cctgccaatg
     3481 gtcttcgata tcttggtaca aagttccacc catggcaacg ttaaagagtt gggtaccacg
     3541 gcagacagag aaaatgggct ttttctgttt aatagcttcc ttgatgaggg ccagttcgaa
     3601 gatatctctt tgaaggttat agtcatcgct atcaatggtt ttgggttcgc cataaaattt
     3661 tggatcgaca ttttgcccac ctgtcaagat gagcttgtca atcaaactga tatagtggca
     3721 ggccatttct tgatcaccaa tcggtaggat gatgggaatc cctccagcat ctttaacgcc
     3781 ttcaacaaag ccttttgctg cgtagctcat catgatgtca tcatctggat gagctttttc
     3841 gtttcctgta atcccaataa ctggtttttt cataaaatga ttttcgcttt ctaatcctct
     3901 tttcgcatga agtagaggag ggtttggagt tcacttgtca aatcgacata ctgaacgacc
     3961 acgtcttttg gtaaatgcag atggactggt gaaaaactga gaattccttt cacaccagca
     4021 tcaaccaaga gattagcaac ctcttgtgac ttgacgctgg gaacagttag gatagcagtc
     4081 ttcacatcag catccttgat tttatccttg atctgagaaa tcccgtaaat gggaatcccg
     4141 tcaggagttt gggtaccgac ttcaggatgg tcgtctaggt caaaggccat gataatcttc
     4201 atcttgttac gttcgtggaa gcggtagtgg agaagggcat ggcccatatt tccaatacca
     4261 accagcatga cattggtaat agagttgtca ttgagcaaat cggcaaaaaa tgtcattagt
     4321 tttttgacat catagccaaa accacgacga ccaagttcac caaaatagga aaaatcacga
     4381 cgtacggtcg ctgaatcaat accgatagcc tctgcaattt gcttagagtt ggcacgttca
     4441 atcttttctg catgaaatct cttaaaaatt cgatagtaga gagagagtct ttttgctgta
     4501 gcttttggaa tagcaaactg tttatctttc acaaaatcac aacctttcta ttcttctatt
     4561 ttatagaaac attgtgaaaa aatcaacaaa aataagaaaa aactaagaaa aatcttagtt
     4621 ttgatgtaaa aaatctgcat gagatagaaa acggtagagg tctccgacca gaccctgata
     4681 aactttttgg cccctaaaag tcagagaagt cacataaagt gtatctggta aggttacaca
     4741 tcctgacaaa gtcaacatga gagcctcatg atcctcatac ttgagagtac gctctacatg
     4801 atagcagtcc ttataggtca gttcaaacat tttggctcta tctttccgat tttgtaaaga
     4861 caccacgttc taccaagcta tccatgagga agtagaattt ttcctgatga atatggtggt
     4921 cttctgattt gaaaatatca actagacgaa ggctaaactt gtcagtgata ttgattttag
     4981 cccctgtaag ttccttgtta atgatgattt tgagttggaa gccttcaccg ctgtttggca
     5041 ctttttccaa aaggcgagtc agttcatagt taccaacctt agtttcaaaa aaggtgttat
     5101 ctttgagggt gaatttttta acagaagggc taagagtgta atcgtaacga caatttttta
     5161 actgaatgat tttttcaaat gccatatggc taacctccga taatttcttt taaggttttt
     5221 gcgagggttt gtaggtcttc aacggtattt tgtggcgaca aactgatgcg aagggattcc
     5281 ttcaagcgtt ctgaatttgc gccatacatg gcttcaagaa catggctgga ttggacaacg
     5341 cctgcagtac aggctgagcc agtagagatt gaaattccag ctaaatctag ccgaaggagt
     5401 aagaggtcat ttttctgacc aggaaatcca atattgagaa cataagggag atgatgtttt
     5461 cctctattca agtaatactg aatgccctcc agctctgcca gaaaggcggt ttctagattt
     5521 tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt cttttagggc tgcaaccatg
     5581 cctacgatag caggcagatt ttcagttcct gcacgttttt tctgttcctg gtctccgcca
     5641 tgtagatagg aatcaaagtc catgctagat gcgtagagaa aaccgattcc cttaggacca
     5701 tggaatttgt gggcagaagc agtgagaaaa tcaatgccca attcttctga atgaattggg
     5761 attttaccaa tagcctgaac tgcatcaaca tgataggcag cagggtgttg cttgagtatt
     5821 tggccaattt cagcgatggg cagtaggttt cctgtctcat tattggcaaa catggtagaa
     5881 accaaaatcg tatcgtcacg taaagccttt tgaatttgct gggctgtgat ttcttgattt
     5941 tctggctgga taatggttgc ttcaaaccca aagtgttgaa ccaagtaatc aattgtttca
     6001 aggacagcat ggtgctcgat ggcagttgtg atgatatgtt ttccttgttc ttggtgacga
     6061 agacagtagc caatgatggt agtattattg ccttcagtcc caccagaagt gaaaaagata
     6121 tgttgaggtt ttgtccttag taactgggct agttcctgac gggcttctcg caagagtttg
     6181 ccagcttgac gaccatgacc atgaatacta gaaggatttc cgtgggtttc ttgcataacc
     6241 ttggtcatag ctgaaatagc aactgctgac ataggagtcg ttgcagcatt gtccaaataa
     6301 atcaaagaat caccttattt ctttttattg taggcaaaga gtgggctgac tggttttctt
     6361 tcgtgaatac ggacgatagc atcaccaatt aactcactag cagtgatgta gcatacattt
     6421 ttaggagttt tttcttttgt tgctactgaa tcagtcacaa gaatttcttt aatattagta
     6481 ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt ggctagaaac agcataaatt
     6541 tctgtagctc cttcacgttc aacgatttta gaagcttcag agaaggtacg tcctgtattt
     6601 aaaatatcat caatcaagat agctttctta ccttcaacat caccaataat ataaccttcg
     6661 ttacgagttg catcgtcttg agggtagtcg ataatggcga taggagcatc aagatattca
     6721 gccaggctac gcgcacgttt gacacctgaa tttttagggc taacgacaac aacatctgaa
     6781 ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg gaacagtgaa aagattatcc
     6841 actggaatat caaagaaacc ttgaacctga acggcatgca aatcaagagt caggatacga
     6901 tcaactccag ccttaaccag catattggca actagttttg ctgtaagtgg ctcacgagga
     6961 caagcaatgc ggtcttgacg tgcatagcca aaatatggaa ggacaacgtt gatactgtgg
     7021 gcacttgcac gcacacaagc atcgaccatg attaacaatt ccattaggtg gttgttgaca
     7081 gggaaacttg ttgattggat gatgtaaaca tcataaccac ggacactttc ttcgatattt
     7141 acttggattt ctccgtctga aaattgacgt gatgatagtt ttccaagtgg gacaccaaca
     7201 gcttgggcaa ttttttgtgc aatctcttgg ttagagttga gtgcgaaaag tttcatgttt
     7261 tttctatctg acattataga ccgtcctctg taaactttat aaatcctagt tatatttacc
     7321 ttacatatat gaactgggat ttgtgtattt ttatcttttc tattttacca aaaaatggag
     7381 attatttcag ctatttttca tacttttgac aaatcgaacc aattttgaag gagctttttg
     7441 gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc tgtttgcctt gctcatgatt
     7501 ttccacttca agctccaatt cgtaatctgt tatatcaaag tatcggctct gatccagtgc
     7561 catgagacca atagctgttt tcatttcata gcgaagcgtt gttagacaac caagaacctg
     7621 ccagttctta ctttggatac catgtttcgc caattcatcc agtactagtc cttgaggaag
     7681 ttcttcctta ctcagatagt cctcagcatc ttttagttgc aatttttggt tgtattccat
     7741 gtttccaaca ctctgcggga ctttgagtgt caactcagcc cagtcttcaa aggttcgaat
     7801 gcgcatagcg actttctttt ctcgcagttc aaaatcaggc gtgtcgatgt agtaatttgt
     7861 ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga cgattgtatt catctttttt
     7921 caatagtgtt ttcaattcaa tttctaaatg tttcattttt cttacctttt tttatcgttg
     7981 aaagcggatt tatggtataa taagcattgt atttattgta tatgaatctg gagaaaaaat
     8041 caaagatatt tttgacggat aatatgagaa caagggagaa tatatgacct tagaatggga
     8101 agaatttcta gatccttaca ttcaagctgt tggtgagtta aagattaaac ttcgtggtat
     8161 tcgtaagcaa tatcgtaagc aaaataagca ttctccaatt gagtttgtga ccggtcgagt
     8221 caagccaatt gagagcatca aagaaaaaat ggctcgtcgt ggcattactt atgcgacctt
     8281 ggaacacgat ttgcaggata ttgctggctt acgtgtgatg gttcagtttg tagatgacgt
     8341 caaggaagta gtggatattt tgcacaagcg tcaggatatg cgaatcatac aggagcgaga
     8401 ttacattact catagaaaag catcaggcta tcgttcctat catgtggtag tagaatatac
     8461 ggttgatacc atcaatggag ctaagactat tttggcagaa attcaaattc gtactttggc
     8521 catgaatttc tgggcaacga tagaacattc tctcaactac aagtaccaag gggatttccc
     8581 agatgagatt aagaagcgac tggaaattac agctagaatc gcccatcagt tggatgaaga
     8641 aatgggtgaa attcgtgatg atatccaaga agcccaggca ctttttgatc ctttgagtag
     8701 aaaattaaat gacggtgtag gaaacagtga cgatacagat gaagaataca ggtaaacgaa
     8761 ttgatctgat agccaataga aaaccgcaga gtcaaagggt tttgtatgaa ttgcgagatc
     8821 gtttgaagag aaatcagttt atactcaatg ataccaatcc ggatattgtc atttccattg
     8881 gcggggatgg tatgctcttg tcggcctttc ataagtacga aaatcagctt gacaaggtcc
     8941 gctttatcgg tcttcatact ggacatttgg gcttctatac agattatcgt gattttgagt
     9001 tggacaagct agtgactaat ttgcagctag atactggggc aagggtttct taccctgttc
     9061 tgaatgtgaa ggtctttctt gaaaatggtg aagttaagat tttcagagca ctcaacgaag
     9121 ccagcatccg caggtctgat cgaaccatgg tggcagatat tgtaataaat ggtgttccct
     9181 ttgaacgttt tcgtggagac gggctaacag tttcgacacc gactggtagt actgcctata
     9241 acaagtctct tggcggtgct gttttacacc ctaccattga agctttgcaa ttaacggaaa
     9301 ttgccagcct taataatcgt gtttatcgaa cactgggctc ttccattatt gtgcctaaga
     9361 aggataagat tgaacttatt ccaacaagaa acgattatca tactatttcg gttgacaata
     9421 gcgtttattc tttccgtaat attgagcgta ttgagtatca aatcgaccat cataagattc
     9481 actttgtcgc gactcctagc cataccagtt tctggaaccg tgttaaggat gcctttatcg
     9541 gtgaggtgga tgaatgaggt ttgaatttat cgcagatgaa catgtcaagg ttaagacctt
     9601 tttaaaaaag cacgaggttt ctaagggatt gctggctaag attaagtttc gaggtggagc
     9661 tattctggtc aataatcaac cgcaaaatgc aacgtatcta ttggacgttg gagactacgt
     9721 taccattgac attcccgctg aggaaggctt tgaaaccttg gaggctattg agcatccatt
     9781 agatattctc tatgaggatg accactttct agtcttgaat aaaccctatg gagtggcttc
     9841 tattcctagt gtcaatcact ctaacaccat tgccaatttt atcaagggtt actatgtcaa
     9901 gcaaaattat gaaaatcagc aggttcacat tgttaccaga ctagataggg acacttctgg
     9961 cttgatgctc tttgccaagc acggttatgc ccatgcacga ttagacaagc agttgcagaa
    10021 gaaatctatc gagaaacgct actttgcttt ggttaaggga gatggacatt tggagccaga
    10081 aggggaaatt attgctccga ttgcgcgtga tgaagattcc attattacca gacgagtggc
    10141 taaaggcgga aagtatgccc atacttcata caagattgta gcttcttatg gaaatattca
    10201 cttggtctat attcacctgc acactggtcg aacccatcaa atccgagtcc atttttctca
    10261 tatcggtttt cctttgctgg gagatgattt gtatggtggt agtctggaag atggtattca
    10321 acgtcaggct ctgcattgcc attacctatc cttttatcat ccatttttag agcaagactt
    10381 gcagttagaa agtcccttgc cggatgattt cagtaacctt attacccagt tatcaactaa
    10441 tactctataa aaactgtctc agagtataat tattatctta aaggagaaaa ctcatggaag
    10501 tttttgaaag tctcaaagcc aaccttgttg gtaaaaatgc tcgtatcgtt ctccctgaag
    10561 gggaagagcc tcgtattctt caagcaacaa aacgcttagt aaaagaaaca gaagtgattc
    10621 ctgttttgct tggaaatcct gaaaaaatta aaatttatct tgaaattgaa ggaatcatgg
    10681 atggttatga ggtcatcgac cctcaacatt atcctcaatt tgaagaaatg gtttctgcct
    10741 tggtggagcg tcgcaagggc aaaatgactg aagaagatgt acgcaaggtt ttggttgaag
    10801 atgtcaacta ctttggtgtg atgttggttt acttgggctt ggttgatgga atggtgtcag
    10861 gagcgattca ctcaacagct tcaacagttc gtccagctct acaaatcatc aaaactcgtc
    10921 caaatgtaac tcgtacttca ggagccttcc tcatggttcg tggtacggaa cgttacctat
    10981 ttggagactg tgccattaac atcaatccag atgcagaagc cttggctgaa attgccatca
    11041 actcagcaat cacagctaag atgtttggca tcgaacctaa aattgccatg ttgagctatt
    11101 ctactaaagg ttcagggttt ggtgaaagcg ttgataaggt cgttgaagca actaaaattg
    11161 ctcacgactt gcgtcctgac cttgaaatcg atggtgagtt gcaatttgat gcagcctttg
    11221 ttcctgaaac tgcagctctg aaagctcctg gaagtacggt agctggtcaa gcaaatgtct
    11281 tcatcttccc aggtatcgag gccggaaata tcggttacaa gatggctgaa cgcctgggtg
    11341 gctttgcggc tgtaggacct gttttgcaag gtttaaacaa gccagttaat gatctttctc
    11401 gtggatgtaa tgcagatgat gtttacaagt tgaccctcat cacagcagct caagcagttc
    11461 atcaatagtg aaaactataa agcgatatac tatgctatac tgtagttatg aaactatgta
    11521 cgaaaagcac tgccattaat tcctgagaac taaattactg attggtgtca aaaaggaaaa
    11581 cttccaagcg atgatatcct gtctatacac gacctataga aatctgtaat atgtatgtcc
    11641 gtaaaacgat aatttccctt tttgatttta aatgagtatg aaaagagaat tttttcatga
    11701 gaaaacgcat aatattaagt gtcttgacac ctgctattat gcgttttctg gctctttgtc
    11761 aactgtagtg ggttgaagaa aagctaagct cgagaaagga caaattttgt cctttctttt
    11821 ttgatattca gagcg
//
LOCUS       DAVBCR010000062        11195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCR010000062 DAVBCR010000000
VERSION     DAVBCR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.062"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..869)
                     /locus_tag="VQ508_001597"
     CDS             complement(<1..869)
                     /locus_tag="VQ508_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2490568.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQN"
     gene            1493..1675
                     /locus_tag="VQ508_001598"
     CDS             1493..1675
                     /locus_tag="VQ508_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2490569.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1685..2050
                     /locus_tag="VQ508_001599"
     CDS             1685..2050
                     /locus_tag="VQ508_001599"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2490570.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            2047..3237
                     /locus_tag="VQ508_001600"
     CDS             2047..3237
                     /locus_tag="VQ508_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2490571.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3752..4744)
                     /locus_tag="VQ508_001601"
     CDS             complement(3752..4744)
                     /locus_tag="VQ508_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490572.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            4904..6658
                     /locus_tag="VQ508_001602"
     CDS             4904..6658
                     /locus_tag="VQ508_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490573.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6642..8363
                     /locus_tag="VQ508_001603"
     CDS             6642..8363
                     /locus_tag="VQ508_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490574.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8374..9003
                     /locus_tag="VQ508_001604"
     CDS             8374..9003
                     /locus_tag="VQ508_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2490575.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            8904..9461
                     /locus_tag="VQ508_001605"
     CDS             8904..9461
                     /locus_tag="VQ508_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2490576.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9449..10813
                     /locus_tag="VQ508_001606"
     CDS             9449..10813
                     /locus_tag="VQ508_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490577.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4101 a         1574 c         1896 g         3624 t
ORIGIN      
        1 aagttttgtt tgatggcttc aaacttttca gaactttcaa gataggcatc cacagccgcc
       61 ttgtagcagt tggttactgt tgatacgtag tgaatagact tcatacgtcc ttcgattttt
      121 agactgtcca caccattttc aatcatatct ggaatgtggt caatcataga catatcaacg
      181 gctgacattg aaaattcttc tagaatctca cccttcaaac tcttacgttc tttcccaaat
      241 ggcatatcgt aaaggtcgta tttccaacgg catgactgag aacacccacc acggttggca
      301 tcacgcatac tcatgtggtt tgaaagagta caacgtccag agtatgaaat acacatagct
      361 ccatggacaa aggcttcaat ttcaacatct gtacgtttgc ggatttcagc taattctttc
      421 attgaaacct cacgtgctaa aacgacacga gtcaagccta gctctttcca gaactcaagg
      481 gtttcatagt tagtggcact ggcttgggta gaaaggtgga tttcaaggcc tggtgcttca
      541 gtcgctgcaa tcataatcaa ggctgggtca gatacgataa ctgctgcaat cccgatatca
      601 cgcagtttac gaaaccactc accagcacca gcttcatttc cttcgtgcat aaccatatta
      661 gccgctacat agaccttggc accatacttg gccgcaaact gcacgccttc ttccatctgt
      721 tcgaaagtaa agtttcccgc acggctacga agaccatagg cctgaccacc gataaagaca
      781 gcatctgctc catactgaac agctaccttt agcttctcta aagtccctgc aggtgataaa
      841 acctcaggac gttttaatgt ttttgtcatg atttctccta tttgcaatat aaaagtttga
      901 catttttcct tctcatttta tagtaaataa aggataaaat caagcctaga cggattgttt
      961 tgacaattct aatcttttag actttgcata ataactatta aacagtcgaa aattacccca
     1021 gttttaacaa gtagtcaaaa agtctaaaaa agtaaaaacc agtatcccaa gatactgact
     1081 cgtaaaaata tataaattag tccttagatg actactccca ctctgttgct cctataatgg
     1141 aaaaggattg atacagtcta gaatatcgct tttttattta ttattctgat ataaaactgt
     1201 tagaattgat tttttgtttt atacccaaat atgaatagag acgtagctaa taagttgaat
     1261 aacatatttg gaaaaatgag aaatagcaat attactagaa cttttagaaa caatcaatat
     1321 ttaaaaagaa aaaagctcaa agaatttatt aaaccatgtt ataatagata tagctataat
     1381 tttatttata acaagaggat agaaatgacc gaattagaaa gaaaaaatcg aaaaattagc
     1441 taagaaatat tctgataact taaacatcaa agttcaagag agagttcgtg aaatggcaaa
     1501 tgataataag agccattatt tgatatacag agttttaggt atttcatttg aagaaggaga
     1561 aaatatcgat ttgtatcaaa ataaaggtcg ttttttatac aaatatgctg gttcattttt
     1621 agaagaagct gcagtactat cctttaacga aaaatttggt acagaaaata cttaaaaagt
     1681 taacattcct aattctgaaa atacaaaacc taagactttt gaaattgatt gtttagtcgg
     1741 agaaaaacac gcatacgaaa taaaatggtg ggatgcaact acagatggag accatataac
     1801 taaagaacac actagaataa aagttattca taacaaagga tatataccaa ttcggttaat
     1861 gttctactat ccaaatagaa ctcaagctat aaaaattcag caaactttag aaacattgta
     1921 taacggtatt ggagggaaat attattatgg agattctgcc tgggaacatt taagagcagt
     1981 gaccggtatt gatttactta gtattctaac agatattgca aataaaaaaa caggggtaaa
     2041 atcaaaatga cagtattaaa aggagataac ttagaaatat taaaaactat tgaatcctca
     2101 agtattgatt taatctatat ggaccctcct ttctttacac agaaaaccca aaaattatct
     2161 aataacaaaa atattatgta ttcattcgaa gatacgtgga cttcgattga ggattacaaa
     2221 gaatttttgt ctgtaagatt agaagaatgc aaaagagtgc taaaaaatag tggcagtatt
     2281 ttcgttcatt gtgataaaat tgcaaatcat catattagat taattttaga taatatcttt
     2341 ggagcagata tgtttcaaag cgaaattata tggaactata aacggtggtc taattcaaaa
     2401 aagggattat tgaacaatca tcaaaacatt tacttttatt caaagtcaaa agattttaaa
     2461 tttaatacaa tttttacaga gtattcttct actacaaata tcgaccaaat actagtagaa
     2521 cgaaaacgaa atggaaactc taaaactata tataaagttg ataataatgg taactatatt
     2581 ctagcaaaag agaaaaatgg agttcccctt tcagatgttt ggaatatacc atttcttaat
     2641 ccaaaagcta aagaaagagt aggttatcct acacaaaaac ctattctgtt attagaacaa
     2701 attataaaga ttgctactga taaaaatgat atagttttag acccgttctg tggaagtgga
     2761 actactttag tagcctccaa gattttgaat agaaattata tggggattga tttatctgag
     2821 gaagctatca atataactca gcaacgtctg gaaaatgtta taaaaacaag ttcaaattta
     2881 ttgaataaag gaatcgaagc atatagaacc aaaactgagg aagaggaaaa cattcttaaa
     2941 ttattacagg caaaaattgt tcaaagaaat aaaggaattg atggtttttt acctaaacat
     3001 tttcaaaaaa aaccgatacc tataaaaatt caaaaaaata atgaatgtct gaatgagagt
     3061 atctctttat tacagaatgc tataaactcc aaaaaacttg attttggagt agttataaaa
     3121 actcattcag ataatttatt atttgatttt gatacgattc ctgaaaatat aatagtagtt
     3181 gaccattttg aacttacaat cgaaaaatgg ttaagtaaaa gtcaacagtt actatagttt
     3241 gcaaattatt tacagaaaga acaaaaaatg ctctgaaaca ctgatttcaa ggcatttttt
     3301 gttagtttaa aattactccc attcttcttt tccaaacgta caatatatcc aaaaccattc
     3361 aaaatactag attctatttt ttataatatc actaaatcca cctaattata ggacgttttc
     3421 agatttttag tcccgtccca gtcccagtac cggagaaata ttgttttaat ataatatctc
     3481 tttttgtctt ctaagctctt aaaagcaaaa gaacaagtaa agagtcaaga caaggataaa
     3541 aagtccatat tagggcaaat aaaaagcttt aagacagatg acaaatctaa gtcaaataag
     3601 aaagaccata gcaaaggtgc agagagataa atattggcgg tcttcggact gcctttattt
     3661 ttttatccat ttttcaaatc aaatttattc agactatata tgcacatata cacttaaatt
     3721 catataaaaa catggcttgt aaaaaattac tttaatcaca ataatcgcat ttaaaattgt
     3781 gatgtttgca agctaaatta cggacttcac ttggaagttt tcccttgtat cttttataat
     3841 agatagaaaa tttgctggca gatgaatatc caacagattc tgctatctct tttataggta
     3901 gttcagtgtt taaaagaaga gtttcagcta cattcattct ttttctttga gtgtactctg
     3961 taatgctttg acaatatttt tccttaaata aattttttaa tttagtacca ctcattttag
     4021 atattttttc aagcgtgcct tgatttacat tcgttgcaaa atgatcatct aagaatcttg
     4081 ctacatcttc aagtgcttta tcatcatcaa tttcaatttt atattttttt ctatttaagt
     4141 atgtgtcaat tactatactt atccattcat ttgcctttgc tttaaagaaa aaatcagcgg
     4201 caggagcgtc catcttacaa tttaatattt ccattgccac tctttctaag gcctttgtaa
     4261 gtattatttg attcggttga agcaaggttg aataaaaaga ttctggatta atgttaatag
     4321 atgctaaatg tttttctatt agctcttttt taaaaccaat ggaaacagca agataacaac
     4381 aattctcgtg taataaaaaa acaaaatcat cttttatatt atcaaaatca aaagtacata
     4441 aagagtttgc ggtaatagtt tgatacggat taaacttttc tccgtttgca ctgacaatgt
     4501 aacttgaata aattgaaaca tagtctgaca tactataagt gctattttga actacttcct
     4561 ctttgatata aaaatcatgt atatcgataa tgaagctgcc tccttcataa aaccaatata
     4621 ggccttctgc ataagttgaa ttctcttttt tccaacaaaa tgtatgacct gcacttgaat
     4681 acttctcatt gtttttacat tcatctactt tcatataatc ttttacaaaa tcataatatg
     4741 acataacaca ctatcccttt tagacaatat tccaattagc cttattaatt caaaactatt
     4801 gtattagtaa ttataacaga tgtataatag aaaagcaatg atagatatta tcaattaagc
     4861 gaatttatat ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa
     4921 actatttgct tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc
     4981 tatatctgct gtacttacag tatatggata ttatttaatc tacaaatttc tagataagtt
     5041 aataattaat tcaaacttat ccggtgcaga gagtatagca ttaaaatctg ttattacact
     5101 aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacatatct tgggatttag
     5161 gcttgaaaca aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt
     5221 ctttgactta aatccatctg gtcaaataag aaagatcata gatgataatg cagcacaaac
     5281 tcatcaggtg gtagctcaca tgattccaga tagctctcag gcaatagtca cacctatact
     5341 tgtccttgta cttggcttta tagtaagtat aagagttggc ataattttgc ttgctcttac
     5401 tataattggt ggcttaattt taggggcaat gatgggcgag caagaattta tgaagatata
     5461 ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt
     5521 tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg cgataaaaga
     5581 ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca
     5641 atggttattt tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag
     5701 cttagctagc gcagaggtga ttttacttga gcttatcatg attttatttt tatcaggagt
     5761 tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta
     5821 tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg
     5881 taatgtcaat aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa
     5941 tgataaagct atcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact
     6001 tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa
     6061 tgttaataaa ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc
     6121 cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta
     6181 tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa
     6241 attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg
     6301 ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat
     6361 tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa
     6421 cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat
     6481 tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa
     6541 aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct
     6601 gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa
     6661 aaagatttgc tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat
     6721 ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag
     6781 ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga
     6841 tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag
     6901 aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact
     6961 tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag
     7021 agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa
     7081 tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta
     7141 ttttaagctt tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat
     7201 attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg
     7261 agattaaagc atataattta tcgaaggata ttaaagatga cttatataaa aaaatggaag
     7321 atagtgagag aatacactta aaggcggaag taactacaat tttaactttg tctatatctt
     7381 caatatttag ctttatatct cttgctgttg tgatatttgt cggcgtaaat ctaattatta
     7441 ataaagagat aaattctctc taccttatag gatatttact agctgctatg aagataaaag
     7501 actctttaga tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag
     7561 aaagattaaa agaaattcaa aatcaagatt tacaagaagg cgatgactat agcttaaaaa
     7621 aatttgatat tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa
     7681 atggtgtaag ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct
     7741 gcggtaaaac aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa
     7801 tcttaatcga tggcaaagat ataaaagaaa tatcaacaga atctcttttt gataaggtct
     7861 ctatagtttt ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg
     7921 gtaagcaaga tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag
     7981 attttataga aaaaatggat aaaggtttcg atacagttat tggtgaaaac ggagctgagc
     8041 tatcaggagg agaaagacaa agattatcaa tagccagagc cttcttaaaa gatgcgccga
     8101 tattgatctt agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag
     8161 agtctttaaa taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat
     8221 ccatagaaaa tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta
     8281 agcatgaaga gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa
     8341 tggcagaaga atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa
     8401 gatttagtaa gtatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt
     8461 atgatgggct tgattccaat attgttctta atatacccga cagtattagc catagttgca
     8521 ggaactgttg ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt
     8581 ggtatgatat caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta
     8641 tcacttatag taatgataat agcagaatta attagaaaga ttggtaatta taattcattt
     8701 aaatacaata tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa
     8761 atgcttttag caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat
     8821 gttgatgtat tagaaaagtt aataacttat cctcacatgg ctttaatagc cttaggtgct
     8881 ttcttaggag gaattcttgg agcatatata ggcaaggctc tattgaaaaa acacttttca
     8941 aatggattat attgtgtggg atactttact ccttgcctaa ttttatggtg ttatctgaat
     9001 taaaccctat agttaagatg tttttgagta tacctattgt tattagaatg tttattttac
     9061 catttatggc agcaagcttt atgataaaga cctcggatgt aggcgcaata atttcatcga
     9121 tggataagct taagatttca aagaatgtat ccatacctat tgcggttatg tttagattct
     9181 tcccatcttt taaggaggag aagaaaaaca tcaaaatggc tatgagagta agagggataa
     9241 attttaaaaa cccagtcaaa tatcttgaat atgtttctgt gccactactc attatatcat
     9301 ctaatatatc agatgacatt gcaaaagcgg cagaaacaaa ggcaatagaa aatccaattg
     9361 ccaagaccag atacattcgc gtaaagatac agctaattga ttttgtttat gttttagcgg
     9421 ttgctggact tattgtggga ggcttaatat ggttgaaata aaaaatttaa gtcttgatta
     9481 tggtgaagag catatattag atgatatatc actatccata gccgagggag agtgcgtgct
     9541 atttacagga aaaagtggaa gtggtaagtc atctttaata aattcaatca atggactagc
     9601 tgtaaggtat gataacgcaa agacaaaggg cgaaataatt attgatggta agaatataaa
     9661 aaatttggaa ctttatcaaa tctcaatgct tgtttcaact gtttttcaaa atcctaagac
     9721 atattttttt aatgtcaata cgacattaga attattattt tatttggaaa atatcggtct
     9781 tgcaagagaa gagatggaca ggcgtttgaa ggatatactt gagatattcc cgataaaaaa
     9841 tcttttgaac agaaatatat ttaatctatc cggcggtgaa aaacaaattc tttgcattgc
     9901 agcttcttat atagcaggta caaagattat agttatggat gagtcttcat cgaatttaga
     9961 tattaaaagc ataagtgttt tggcaaagat gctaaagata ttaaaagaga aaggcataag
    10021 cataattgtt gcagagcata gaatttatta tttgatggac atagttgacc gtgtattttt
    10081 aatagataaa ggaaagctta aaaaaactta tactagaagt gaatttttaa agctagataa
    10141 aaatgaatta aatgctttaa gtttaagaga taaagaatta agtaaattaa aagttcctta
    10201 tttaaaagaa ggtggagagt atcagataaa aaatcttagc tacaaattta ctgatgatga
    10261 gtgtttaagc ttaaaagata tttcgttcaa gcttgggaaa atttatggca taataggatc
    10321 caacggacga ggaaaatcaa cgcttttaag atgtttaata ggtcttgaga aaaaatcaaa
    10381 agaagaaatt tattttaagg gagagaagct atctaaaaaa gaaagactca aaaactcttc
    10441 acttgttatg caagatgtaa atcatcaatt attcacagat gaagtattca acgagcttag
    10501 attaggagta aagaattttg atgaagaaaa ggcgaaaatc attttaaaag atttaggcct
    10561 ggacgaattt attgaaaggc atccgatgag tttatcagga gggcaaaagc aaaggcttgc
    10621 aatagcatct gttatgtgca agacttctcc atttatcttt tttgacgaac cttcaagtgg
    10681 tatagattat tccaatatga taaaaatatc tgaactgatt aataagtata aaaccatgga
    10741 taaaataatt tttattgttt cccatgatat agaattttta aatgaagtgg cagatgaaat
    10801 ttttgaattg taaaatagag tctgttaaac atgtctatag aactggtaaa ttgtctaaaa
    10861 atttaaaaga tactaacaca tattgatatt tattagaaaa ttaacttcag gatttttaca
    10921 gtatttataa actttataat atgtatttat agtattaata atttccatat ctttagggaa
    10981 attatttatt tacttaccat aattaaagag atgagagata aatatcggca gtcttcggac
    11041 tgcctttatt tttttactta atttaatgca gtaaatcata cgattgaaag gaatctgcgt
    11101 tatatgctat aattaggtag ttaatctata catttagaaa ggtaaaatat agaattggag
    11161 aatgatatgg gattttcata taataaattg taacc
//
LOCUS       DAVBCR010000063        11086 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCR010000063 DAVBCR010000000
VERSION     DAVBCR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.063"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ508_001607"
     CDS             complement(6..890)
                     /locus_tag="VQ508_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2490578.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ508_001608"
     CDS             complement(894..1280)
                     /locus_tag="VQ508_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2490579.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ508_001609"
     CDS             complement(1273..2616)
                     /locus_tag="VQ508_001609"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2490580.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ508_001610"
     CDS             complement(2698..3582)
                     /locus_tag="VQ508_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490581.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ508_001611"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ508_001611"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2490582.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ508_001612"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ508_001612"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2490583.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ508_001613"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ508_001613"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2490584.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ508_001614"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ508_001614"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2490585.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ508_001615"
     CDS             complement(10565..10831)
                     /locus_tag="VQ508_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490586.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11086)
                     /locus_tag="VQ508_001616"
                     /pseudo
     CDS             complement(10915..11086)
                     /locus_tag="VQ508_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3096 a         2540 c         2193 g         3257 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc atttca
//
LOCUS       DAVBCR010000064        11053 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCR010000064 DAVBCR010000000
VERSION     DAVBCR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.064"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            1..486
                     /locus_tag="VQ508_001617"
                     /pseudo
     CDS             1..486
                     /locus_tag="VQ508_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(602..1405)
                     /locus_tag="VQ508_001618"
     CDS             complement(602..1405)
                     /locus_tag="VQ508_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2490587.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1684..2439)
                     /locus_tag="VQ508_001619"
     CDS             complement(1684..2439)
                     /locus_tag="VQ508_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2490588.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2509..5160)
                     /locus_tag="VQ508_001620"
     CDS             complement(2509..5160)
                     /locus_tag="VQ508_001620"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2490589.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(5153..5731)
                     /locus_tag="VQ508_001621"
     CDS             complement(5153..5731)
                     /locus_tag="VQ508_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2490590.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5731..6201)
                     /locus_tag="VQ508_001622"
     CDS             complement(5731..6201)
                     /locus_tag="VQ508_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490591.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(6188..6766)
                     /locus_tag="VQ508_001623"
     CDS             complement(6188..6766)
                     /locus_tag="VQ508_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2490592.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7049..7444)
                     /locus_tag="VQ508_001624"
     CDS             complement(7049..7444)
                     /locus_tag="VQ508_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2490593.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7540..7794)
                     /locus_tag="VQ508_001625"
     CDS             complement(7540..7794)
                     /locus_tag="VQ508_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2490594.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7803..9137)
                     /locus_tag="VQ508_001626"
     CDS             complement(7803..9137)
                     /locus_tag="VQ508_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2490595.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9137..9370)
                     /locus_tag="VQ508_001627"
     CDS             complement(9137..9370)
                     /locus_tag="VQ508_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2490596.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(9639..9779)
                     /locus_tag="VQ508_001628"
     CDS             complement(9639..9779)
                     /locus_tag="VQ508_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490597.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(9776..9958)
                     /locus_tag="VQ508_001629"
     CDS             complement(9776..9958)
                     /locus_tag="VQ508_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490598.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9960..10743)
                     /locus_tag="VQ508_001630"
                     /pseudo
     CDS             complement(9960..10743)
                     /locus_tag="VQ508_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3230 a         2440 c         2043 g         3340 t
ORIGIN      
        1 ggaaaagtgc tacacagatg tgactgaatt tgccattcca gcaagtactc aaaagcttta
       61 cttatcacca gttttagatg gctttaacag cgaaattatc gcctataatc tttcaacttc
      121 acccaactta gaacaagtac aaacaatgtt ggaacaggca ttcacagaga agcactacga
      181 gaatacgatt ctccatagtg accaaggctg gcaataccga cacgattctt atcatcggtt
      241 cctaagagta agggaattca agcatctatg tcacgcaagg gaaacagcca agacaacggt
      301 atgatggagt ccttctttgg gattctgaaa tcggaaatgt tttacggtta tgagaagtcg
      361 tttcagtcgc ttaagcaatt ggaacaagcc attatagact atattgatta ctacaacaat
      421 aaacgaatta aggtaaaact aaaaggactt agccttgtgc aatacagaac taaatccttc
      481 ggataaatta tttgtctaac tttttggggt cagtacacct aaaactttga tgatatacgt
      541 tttcttgtga gaatatttac ttcatttttg cctaaaattc aatgtttact cagtatttgg
      601 attatgaaaa atcgaggtct aaatctagat acattttttc tgaagacaaa tcattttgac
      661 caccgagcaa gagattttca aaaaaagctg ttaaaaactc agaacgtcgc tgtaaaatct
      721 ttgcattatc taataccaag gcatcacgaa aatatttgga atgttgctga aatggtgtat
      781 tatcaatatc aaaaccaaac tcacgaagat actgaatcaa aaagaccgtt actgtccgag
      841 tgtttccttc gcgaaatgga tgaatctgcc agattcctga aataaaatgc tggatttgtt
      901 taaccacatc cgcctgagtt agtgtcgcat atgcaacttg tttttcctga ttaaaatcat
      961 aatctaaggt catttgaatc atggagtaat cagagtacac aacactttca ccattcaaaa
     1021 caggttcatt ctttgtgata ttggtctgac gaaattgccc caccggaata gagggttcaa
     1081 atatatcttg aaacaactcc ttatgaatag caagtaaggt cgcaggacta aagctaaagc
     1141 ctcttcgaga caatagttct acaatacgta gagaaaccaa gtctgcctct tccgtacttg
     1201 catcaatagt atggtgataa gccgttgcat cctcataaac ctgctcataa gtcagttctc
     1261 cccgggactg tttctcagcc aaagattcca tatacgctga tggcactaga ttgtcaactt
     1321 tctgcagacc aaaacctatc cgccataaat cacgcttcgc ttcataagac aagtttggat
     1381 tgtcaatgtt gtaagttgat tgcatagaaa tatcctttca attgtttttc ttcattacca
     1441 gtaaatcacg aaattggttg gaaactggct taaactataa aaagtcctca caataggttc
     1501 tatgatatat atatatatac ttcatgtaaa taataaaaga gttataatca gcactactga
     1561 taaattcaat accgatttcg ccatcttaaa acttagtcaa acgaataccg ttgatttcca
     1621 gttcattaag tcgttgacct gtcaagactt ctcgttcctt caactggaaa agttaagggg
     1681 gaactaatac aaatcctcta cgcttctata ttcaatgatt tcatttttga taatattctt
     1741 tttaaactca aaatgtttta aaggattatc gattaacact aactttggaa tatcatctag
     1801 atttgtgacc aaggataaaa taaaatccga ttgaaactcc ttgacttttt caaattcttt
     1861 tgcagttaaa cgcacacgcc ccttagattt tcggatacct tttacttcgg caaggtaaga
     1921 atactcttgg acatctacct gaaagtcata tccatcacca taaagacggg catcggttaa
     1981 ctgtccacct tggaattttt cttctttatc aaaatgaagg ataaaataat tctcagcctc
     2041 cattcccgtt tcctgtagac gtttgaattt agttcgaact attggttttt caacgatggt
     2101 tatgcctgtt ttcttccctt cactggcaag taacatttta actatttcag cataactatg
     2161 aacatcttca tttccaaaca tcatatcaat taaatcttta cgaaaacgat aaacttcagc
     2221 tttctgccac caaccttttc gattattgtc aaaataagga tcaaataaat ccattctatt
     2281 ttttacaact ccagttgttt cagcaattcc cagagaaacc acatagcgat aaaactccga
     2341 cttgctagag cattgaaatt cttttataaa aagcttgtca aacttggcaa gaccgtagcc
     2401 aattaaattt aacaattcat aatgagggtg tttggacatc actttctcca tcaatagata
     2461 ttgatttcat tataccaaaa aacacggctc atcaccgtgt ttctgtgttt atttcaccaa
     2521 cttcttcatc tcatcaatac gtgctacggt cgcatcatac ttagcttggt aatctgcttg
     2581 cttgtcgcgt tctttttgga caacttctgg tttagcgttg gcgacgaagc gttcgttgct
     2641 gagttttttg ccaaccatgt cgagttcttt ctgccatttg gcaagttctt tttccagacg
     2701 tgccaattct tcttcgacat tcaagaggtc taccagtggc aagaagattt cggctcctgt
     2761 gatgacgctt gacatagcca gttcaggtgc agggatggtt gatgcgattt ccaagtgttc
     2821 aggatttgtg aaacgtttga tgtagttgac attgctgtta aagaaggctt ccaagtcgct
     2881 atcgcttgtc ttaacaagga tagtgatagg cttgcttgga gcaacgttta cttccgcacg
     2941 cgcattacga acggcacgga tcaagtcttt aaggctttcc acaccagtgt gagcagcaag
     3001 gtcttcaaag gctgggttga cagttgggta ttctgctgta acgatagatc cttctgagat
     3061 ttgtccaaag atttcctctg tcacaaatgg catgattggg tgaaggagac gaaggatctt
     3121 gtccaaagtg tatagtagaa cagaacgtgt gatgactttc tcttcttcgt tatcgctata
     3181 aaggacttcc ttggtcaact caacatacca gttggcaaat tcttcccaga tgaagttgta
     3241 aaggatatga ccagccacac caaactcgaa cttatcaaag ttttcagtaa cttttgcaat
     3301 ggtttcgttg agattgtgga gaatccagcg gtccgtcaca ttaccagcct cacctgttgc
     3361 aacttttgtg acattgtcat gcgccacatc cagcgtcaaa ccttcattgt tcatgaggat
     3421 atagcgagaa atgttccaaa ttttgttaat aaagttccat gaagcatcca ttttctcgta
     3481 agagaaacga acgtcttgac caggagctga gccgtttgac aagaaccaac gaagactatc
     3541 tgtaccatat ttatcaataa catccattgg gtcaatgcca tttccaagag atttagacat
     3601 cttacgtcct tcttcatcac gaattagacc atggataaga gcatttttaa atgggctctt
     3661 tcctgtaaat tcaattcctt ggaaaatcat gcgtgatacc cagaaaggaa taatatcata
     3721 gcctgttacg agtgttgaat ttggatagta gcgtttaaag tcagtagttt cggtattagg
     3781 ccagcccatg gttgagaagg gccagagggc agagctgaac caagtgtcaa ggacatcttc
     3841 gtcctgtttc cagccatcgc ccgctggggc ttcctcgccg acatagattt cgcctgcttc
     3901 attataccaa gcagggattt ggtgacccca ccagagctga cgggaaatca cccagtcgtg
     3961 gacattttcc atccattgca ggaaggtgtc gttgaagcgc ggtgggtaaa attccacctt
     4021 gtcatctgtg tcttggttgg caatggcgtt cttggccaat tggtccatct tgacgaacca
     4081 ttgagtagac aagcgtggct caactacgac acctgtccgt tctgagtgac cgactgaatg
     4141 gacacgtttt tcgattttga caagggcacc gatttcttcc aacttagcaa cgactgcctt
     4201 acgagcttca aaacgatcca tgcctgaaaa ttcaaaggca agctcgttca tagttccgtc
     4261 gtcgttcatg acgttgactt gtggcaagtt atgacgttgg ccaaccaaga agtcatttgg
     4321 atcgtgggca ggtgtgattt tcacgacacc agtaccaaac ttaggatctg cgtgctcatc
     4381 accaacgatt gggattaatt tattagcaat tggaaggatg acatttttac caatcaagtc
     4441 cttgtagcgc gggtcttctg ggttgaccgc aaccgcaacg tccccaaaca tggtctcagg
     4501 acgagttgta gcaacttcaa gggcgcgtga accatcttcc agcatgtaat tcatgtgata
     4561 gaaggcacct tcgacgtcct tgtgaataac ctcgatatca gaaagggctg tgcgagctgc
     4621 tgggtcccag ttgatgataa actcaccacg gtagatccag cctttcttgt aaaggtccac
     4681 aaagacctta cgaacagctt ttgacaaacc ttcgtcaaga gtgaaacgct cacgagagta
     4741 gtctacagag agccccatct tgccccattg ttccttgata gtagtggaat attcgtcttt
     4801 ccattcccag actttcgtca agaaagactc acgaccaagg tcatagcggg aaatgccctc
     4861 accacgcaag cgctcctcta ccttagcctg agtggcaatc cctgcgtggt ccatcccagg
     4921 aagccaaagg gtatcaaaac cttgcatgcg tttttgacgg atgataatat cttgcaaagt
     4981 tgtatcccaa gcgtgaccaa ggtgaagttt acctgtaacg tttggtggtg gaataacgat
     5041 tgaataaggc ttagcctttt gatcgcctga aggcttgaaa acatcagcat caagccattt
     5101 ttggtaacga ccagcctcaa cctcggctgg attgtattta gatgaaagtt ctttagacat
     5161 gtgtgtgtcc tttctctatt ttgtttattt tattttgaat ttgcttagca gcttcttctg
     5221 cagacaaatt cgtattattt attttaaagt agtggtgcaa ctcattcggt tgatattggg
     5281 aatttaattg aagtgtttca gcggtctcta aaatttctct ttcagatacc tcaatatgtc
     5341 gttttaaggg tttgtgcttt aatcgattct ccgttcgatt tcgacgtatg cactcttcaa
     5401 gacttgtttc caactcaaca aaaagaatct cttgatgata atcatccaac aaattctgaa
     5461 tttgctttaa atacatcagt tgatactgat ttgaaaaatc aattacatct gttaaaatta
     5521 ctgatcgctg atttcttgca cttgctccaa ggaatgaaaa agtgattcca cgaacaaatt
     5581 cccacatttc ctctgtatag tcctgataga tttctagtgc aaaatcaatg gcttgatggt
     5641 tataaaatag ggtagcatcc gtcagtcgag ataattcttg accaatggtc atttttcctg
     5701 atgctggagc accaatgatg aaaagatgca tcaaatcacc tcccactcac tcttcagcaa
     5761 ggcatatctc aaatcatcac agcagttgcc ttgagcatct ttacggtctc ttatgcgagc
     5821 ttcgagggta aagccaagct tttccgcgac tcgttgactt tgaaggttat atccaaagca
     5881 agttagttca atcttgtgaa gacccaaatc tttaaaggct aagtcaatca aggcacgcgc
     5941 agcttctggc acataacctc gcccccaata gtctgggtgt aaggtatagc cgatttccag
     6001 cacatcatct tcatggcgat ggttgaaatc gacagagcca acgatcttat ccgttccttt
     6061 gacgacaatc ccgtagcctg ctggataggc gacctctggc aaactagcat agtcaaagat
     6121 atcctcagca tccgccacag tacggactcg taaaacgaga cgttctgttt cgattttatc
     6181 tggcagctca gttcttgcca tccttcttcc tcgctttttt gatgaaactg cccttcatat
     6241 ctacacgctt atccagatag cgataaacgc gctgatatcc atctcccatg aaataggttg
     6301 gggcaaacag ttgattttta aaatgtccct tttcatccag gagttctggg gcaacaagtc
     6361 gctcaagaat cttggcaaag atgtggcaaa taccgtcttc ctcaacaatc ctatctaccc
     6421 gacaatctaa aacaagtgga caggcgtcta aaataggagt ctgagttcgt tcagaaattt
     6481 cataatgcac tcccaaacgt tccaatttct cctgatgact gataaaacca gcctgctcca
     6541 tcgcaagcat agaagtttca tcagaaatat tcacagtaaa tttttgatac tgtttgatct
     6601 gctctgcggc attctctctc gcaacgactc caatcacaac ccaatctcct agactatacg
     6661 aggaactaca ggtcgtgatg ttatagccaa aattctgatc ttgatatcct aaaataaaaa
     6721 caggaaaacc ataatatagt ttattagttt taaatgattg ttttataaca acctcctttg
     6781 actaagacac aaaagaaaag ccctgccatc tacatgacag ggacgaatgt atttatccgc
     6841 ggtaccaccc aatttcgggc caagcccgca tctttagtct ttaaaataaa aagacagaaa
     6901 tatttcattt tttcagtcat tagcaaccag ttttgacaca tttgtggact tctcagcgcc
     6961 gccactttct gtaaaatgtg ggattcaaaa cacctctaat agctctattg tagcaagttt
     7021 ttctcggaaa tgcaaggctc aagaaaaatt acttgaactt gatgccattt tctttcaatt
     7081 tcttgatggt agctgggccg attcctttca aggcaaggag ttctttttca gtccagtttt
     7141 tgaaatctga agcagacttg attccttcat catagaaagt cttggcacga tcaagtggaa
     7201 gtccacccaa gtttgctgca aaatcttcta cagaattagc tactttttga gcttgctctt
     7261 tggtagcttc tactgcttgt tcaacttttt tagaaacagc cttaccagct gcgcttgctg
     7321 actgttctgc acgcttcacg actttttttg tctcatctac aaccttagtc acagtacttg
     7381 aaaaggcacc tgcgcgacgg aggccatttc gtaattgttt tttacgattg agtttctttg
     7441 acatgataaa atcctttcaa taaaaaatcc ctgctctgac aaggatttaa cataaacatt
     7501 ctatcaagat tttagtgaaa aatcaagaat ctttctcgtt taataatttc gctcaccaaa
     7561 caagccttct ggcaaatcat ggaatccctt gcctactttg aagttttcaa acttaccaag
     7621 caagaactga taagcatcca agcgactctc gtagcgaatc atggcatctt tggcacgctc
     7681 gtcctggtct tcttcgtaaa ctttttgact ttccttgtgc gccatgtcgt taatcatgat
     7741 ttgggtattg acccaagctt caaattcctt cataaattct tgttcgtaac tcatttttct
     7801 ccttattcta atccacgaaa atagtccgta tcaatctcac ggtagggctg gatatcctga
     7861 acatactcca acactccttg gaattctccg tcttcatcgt gtactgcagc ataggtgatg
     7921 tggacaaact tacctcgcga ctcagacttg aaccacattt catacttgtc tttgcttccc
     7981 tcacgaagcc tcttcatgat agttttgacc ttgtccaagt acttaggcgg atggcagagt
     8041 tcgacattgc gcccgacttg agacggcgtc cgtttgaaaa tcatctcatc agctggcgta
     8101 ttgtcattgt aatactggaa aatatcttct ttattgacaa aggtaatctc catagggaga
     8161 tgattgagga tgagattggc ctgctcgact gaaagatagc cattaccaaa agcctgttga
     8221 ctatggcggt ccagcacagc ttccttttcc ttaggggtaa aggtaatggt aaaatggcct
     8281 tctggcgtat ctatgacttg ttgaacttga ccttctgccg tatctagctg tacaggctcc
     8341 tctgcaatct tttcctcaat aaagctctgt cgttctggca cccatttctc tgacggacgg
     8401 atgatggcat agccataggc atcgctctcc tccgcaatct gaagccagtc atcctgagta
     8461 aaagactcaa ggagaatcat gaggaggatg gactcttcct tgaaaatcat actttcaaac
     8521 tctgtcgcaa aagcttcaaa agcttccttt acactgctaa ttgacacttc tggtagtgac
     8581 ttgactgtcg ttagagctgt ttgaaagagt tccctaatct gatcatccac tccccacata
     8641 actttgggag gtgaatcgtg tccatagcgc tccatgatag gaaagaagag ttcttcctta
     8701 cgttggtaat ggatgtcaaa ttgccccaca agccccatct gacgcaccaa gcccttgcgc
     8761 atctccgcaa gcatttcctc gtcttccatc aactcatagg tatctaacaa tctacgaatg
     8821 cgaatcaaag ctgcacggag ggccaaattt tcttccttaa agacacgaac tgggtgccca
     8881 gggtgttcgg tatctaaaac ttcgacacct ttgatagcat ttttaaaaag attggcatgg
     8941 acatcacaga gttccataac atcttcaaaa gtgacgcccg aatccgagtt catcagctcg
     9001 tgctccataa gggaaatctc gatggctgac acgcccgtaa aggtcgcatc aaagcgatct
     9061 tgaaccgact caggagaggc gccattgtgc aattctaaca aaatatcccg taggatatga
     9121 atccattcat ctgtcattag tctaatccaa tcacttcgta gccattcgct tccagtgtgc
     9181 gtacaatctt gtccatagga gttcctgtta gcttagaacc ctgtttaagt gatactttac
     9241 gaccaactgt attgcgcatt aagggattgg caaggggttt aaaacccaac tccactagaa
     9301 tttccaagac ttctggatgc ttgtccacca cttctgcaac aggaattgac acatcgatga
     9361 tattgtccat gacgacctcc attgtatgtt ctaatactca atgaaaatca aagagcaaac
     9421 taggaagcta gccgcaggtt gctcaaaaca gtgttttgag gttgtggata gaactgacga
     9481 agtcagtaac catacctaca gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga
     9541 gtataaaatg attttactgc ttatattata ccatatggag agagattaaa aaactagcag
     9601 tggagttcct gctagttttc tgcgacttgg attaccatct agataagttt aaagaaccaa
     9661 tccaatatct tgtaaaacag caattgaact gcaaaagaaa tgatcatcca aaagaatttc
     9721 acaatcccaa taatcggttt gataaaaaga atagcaagaa ttgtccagaa aaatttcatt
     9781 ctctctcctc tggcttaatg agccaccgag ccgtatccat tagcttatct gcaataaaga
     9841 gttttcccag accaacaacg acatggtcat ctttctttat cgttttcatc acttttacac
     9901 cttgcacggt caaaaagtaa ttaccataaa ttttagcaaa agctttgata ggattcatat
     9961 tattctcctt tgatgatttc agcctctttt cggattgttt caacatcttc ttgatattga
    10021 acttcactat agttgactag cttggataat tctttctgca agctttcccc cattggtttc
    10081 atagttgcaa aggttaaacc acctgaaatg ataccaccca agataggaat aaatttcccc
    10141 attcctttgg ccaaccctcc cttggtaaga ttcacaccaa atatttttaa gacttttttc
    10201 aaaatagggt accaaagcgt ctttgttaaa gctttattag gcactgtttt cattacctgt
    10261 ttggcaattg ttataccacc agcacgtagc aaacaaagca gcggttccat tcacccctaa
    10321 cattacgcct agataaagca agagggtatt ttgagcatct tcactcaact cctcacgtga
    10381 agcccaaaga tcctcataac cataaatata acctaattct tgggccaatt tcagagagaa
    10441 accataaaat tgagccacat aagctggaat ggtaattgcc atagcaagcc ctccaggtaa
    10501 tcctgccaaa acagaagtcc cactcgctaa taaaacatta tccctaatac aggcattagc
    10561 cactcgatct aagatttctt gagataagag agacgttgga ccttgttcca ataaggtagg
    10621 aatgtcctgt ggacccaatt ctttggaaaa cttatccact aaaaatttcg aacgatcaac
    10681 cttaacaact ggtagtttta ccacttgttg taatacttgc atagctaagt cttgttcagc
    10741 catatacaaa ctccttattt cttttttgca ccttatcata ttatatttcc tacaaaaaaa
    10801 gcattttcta attagataac attaaaaaat acagaacccc ttttttatag taaaataaaa
    10861 caagaacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagta
    10921 gaggcgtact attctagttt caatatattt taaaagaata tttttccatt tcaaattcaa
    10981 cctattttcc tccaccaaaa aagggctcta taatatttgt agtgggtaaa ttccctatgg
    11041 atattatgga gcc
//
LOCUS       DAVBCR010000065        10989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCR010000065 DAVBCR010000000
VERSION     DAVBCR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.065"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ508_001631"
     CDS             136..2316
                     /locus_tag="VQ508_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2490599.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ508_001632"
     CDS             2368..3183
                     /locus_tag="VQ508_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2490600.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ508_001633"
     CDS             3323..4666
                     /locus_tag="VQ508_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2490601.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ508_001634"
     CDS             5043..6233
                     /locus_tag="VQ508_001634"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2490602.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQ508_001635"
     CDS             6786..7721
                     /locus_tag="VQ508_001635"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2490603.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQ508_001636"
     CDS             7755..8792
                     /locus_tag="VQ508_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2490604.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQ508_001637"
     CDS             8965..9570
                     /locus_tag="VQ508_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2490605.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQ508_001638"
     CDS             9726..10256
                     /locus_tag="VQ508_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2490606.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ508_001639"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQ508_001639"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2490607.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2395 g         3236 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBCR010000066        10721 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCR010000066 DAVBCR010000000
VERSION     DAVBCR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.066"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            51..233
                     /locus_tag="VQ508_001640"
     CDS             51..233
                     /locus_tag="VQ508_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2490608.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            533..1372
                     /locus_tag="VQ508_001641"
     CDS             533..1372
                     /locus_tag="VQ508_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2490609.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1390..3228
                     /locus_tag="VQ508_001642"
     CDS             1390..3228
                     /locus_tag="VQ508_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2490610.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3241..4656
                     /locus_tag="VQ508_001643"
     CDS             3241..4656
                     /locus_tag="VQ508_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2490611.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5248..6294
                     /gene="pheS"
                     /locus_tag="VQ508_001644"
     CDS             5248..6294
                     /gene="pheS"
                     /locus_tag="VQ508_001644"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2490612.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6294..6803
                     /locus_tag="VQ508_001645"
     CDS             6294..6803
                     /locus_tag="VQ508_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490613.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6880..9285
                     /locus_tag="VQ508_001646"
     CDS             6880..9285
                     /locus_tag="VQ508_001646"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2490614.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9458..10459)
                     /locus_tag="VQ508_001647"
     CDS             complement(9458..10459)
                     /locus_tag="VQ508_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2490615.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10615..>10721
                     /locus_tag="VQ508_001648"
     CDS             10615..>10721
                     /locus_tag="VQ508_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2490616.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT         3434 a         1723 c         2280 g         3284 t
ORIGIN      
        1 accccaaaag ttagattttt tctgtctaac ttttggggtg tagttcacag ttgaagagcc
       61 agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt ttggtgaata
      121 agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag atgaccatat
      181 tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga tagagaaaat
      241 ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc attatcacat
      301 tcgaatcaca caaaataaaa aaatttctac aagtacttga caaatagatt gaaatatcat
      361 aaaatgaaaa tggttacaga gttattaatt atttaagctt catgtcacca ttaaaaattg
      421 aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc acatcggaag
      481 aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt ttatgatcat
      541 aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata tcgatattct
      601 tctttttgga aggggcatag cttttggaag aaaaactgga gataaagtaa atccaattga
      661 tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta cagagatgtt
      721 tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc taggtaaaat
      781 aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc atatttcttc
      841 gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct gggaaatatc
      901 gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa taaaaaaaga
      961 gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt ttgttaatgc
     1021 taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta tgggaattga
     1081 gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg attattatag
     1141 attcataact catataaaat tatttgctca tcgcttggtt gaaaatacaa cttattgtga
     1201 cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag aatatgaatg
     1261 tggtgaacag gtggcaatgt ttatacaaac tgagtacaat tatttgctga cttctagtga
     1321 attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt aatagaatag
     1381 gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat tggaggtgaa
     1441 aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt aaaagatgaa
     1501 aatttagtca atgatgatga tgtgaagaag ataccagatg taataggtat tatgaaaaag
     1561 aatggacaat atcaaattat acttggtaat gatgtagcta attattataa agaattcgtt
     1621 aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa tattttagaa
     1681 agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat gttaggggga
     1741 ggtatgttga aagtcttggt aatcatttta ccaatgcttg gtatattgca atcagattct
     1801 cagactattg cttttttgac attttttggg gatgctccat attatttctt accgctgtta
     1861 ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat gtctgtagca
     1921 ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc tcttagttta
     1981 tttggtgcac ctgtgacacc agctagttat ggttcatcag tcgttccaat tcttattatg
     2041 gtttggttga tgaaatatat tgaaaaaata attgctaaat taacaccagc tattactaag
     2101 agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt agttgtagtc
     2161 ggacctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca aatgtatggt
     2221 gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat tgttatgact
     2281 ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac tccagacgta
     2341 ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc gatggctgtt
     2401 gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg tttctcagcc
     2461 ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata taaaaaaccg
     2521 ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct tactagtgtt
     2581 aaagcatatc tatttgctgt cccatctttg atagcgttgc ctcaatttat ttattctgat
     2641 gtgccatcaa atattgtaaa tgctttaatt gtggcggtca tttcggttgt tattaccttt
     2701 gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt agaagttaaa
     2761 gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat cattccgtta
     2821 agcgatgtcc aggataaaac attttcagat aaattaattg gagacggagt agcgattatc
     2881 ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat ttttccgact
     2941 aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca tattggatta
     3001 gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg agacagagtt
     3061 ttcaaaaatc agttgatttt tgaaatgaac ttgaatttaa tcaagactaa aggctacgaa
     3121 acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt agtattacct
     3181 aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg gaggaaagtt
     3241 atgaccattt ttccagatga ttttctttgg ggtggagcgg ttgcagctaa tcaagtagag
     3301 ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc caaaggtgga
     3361 ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg tattgatttt
     3421 tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa tgtttttcgt
     3481 acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc taacgaagct
     3541 ggtttgaaat attatgatga attatttgat gaactacatg ctcacgggat agaaccactt
     3601 gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca tggatggatt
     3661 gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga aaggtataaa
     3721 gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga attaccgttt
     3781 actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt atatcaagct
     3841 atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga gattaattca
     3901 gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac tccaaatcca
     3961 aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc agatgtgcat
     4021 gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga cattaacata
     4081 gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt tttatccttt
     4141 agctattaca tgagtgtgac tcaatctgct attccaacac agtataattc aggagaaggg
     4201 aatattattg gtggtttagt aaatccttat ttagagtctt cagagtgggg atggcaaatt
     4261 gatccaattg gactacgtat aattttaaat agatattatg accgttatca aatcccatta
     4321 tttatagtag aaaatggatt aggtgctaaa gatcaactaa taaaagatga acttaataac
     4381 ttaaccgtcc aagatgatta tagaattcag tatatgaaag aacatttatt gcaagttgct
     4441 gaagctctac aggatggtgt tgaaattatg ggctatacgt cttggggatg tattgactgt
     4501 gtttctatgt ccactgcaca actttctaaa aggtacggtc tcatttatgt tgatcgaaat
     4561 gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg gtataaagga
     4621 gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg attagatatt
     4681 atcacggtta aggcaacatc tcattaaata taaaataaag aggtttggaa gacatcaaat
     4741 tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca gagagccgat
     4801 tataaatagt taacttagga tagattggtt atatatagta gactgaatct aaaatagtac
     4861 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttttca
     4921 tcttatttca atccgctata aagttagata agaataagct aacaagcaga agtaagatag
     4981 gtcttgcaag aatggtaaaa atctgataaa ctaagtagta attgaatagg aatctgaaag
     5041 actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc tctctatatg
     5101 aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact tgtcagatga
     5161 aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg gttttctttt
     5221 tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt aaaagcgctt
     5281 cgcgaagaaa cgctggctag cttgaagcag attactgctg gaaatgaaaa agagatgcaa
     5341 gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct caaagggatg
     5401 aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga agctcgtgat
     5461 gtcttgactg ctgcttttga agaaacagct aagctcttgg aagaaaagaa agtcgcggct
     5521 caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc gactggtcac
     5581 cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg tatgggttat
     5641 caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg tatgaacctt
     5701 ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga agaaatcttg
     5761 ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga tttttctaaa
     5821 ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga cgatgcgacc
     5881 cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat ctctatggct
     5941 gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga gcgtcaaatt
     6001 cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga tgtttcttgc
     6061 ttcaagtgtg gtggagaagg ctgtaatgta tgtaagaaaa caggttggat cgaaattatg
     6121 ggggccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc gactgtatac
     6181 tctggctttg cctttggtct tggacaagag cgtgtagcta tgctccgtta tggaatcaac
     6241 gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa ataatgatta
     6301 gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa gcctttcgtg
     6361 aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa gaggcttata
     6421 gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat ttcattatgc
     6481 atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa actgagagag
     6541 aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc caaggatttg
     6601 gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat agtttttctt
     6661 gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat aaccgatatg
     6721 gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat acggattggt
     6781 tactgagaaa gaaattaagg taagaagaga attcttctat tgaaatgata ggaaaggaaa
     6841 agaaccagtg tggcaatggt cattctggag aactaaatta tgcttgtatc ttataaatgg
     6901 ttaaaagaat tggtgggcat tgatgtgcca tcacaggagt tggctgaaaa aatgtcaacc
     6961 acagggatcg aggtcgaagg tgtcgaatca ccagctgccg gtctttcaaa aattgtcgtc
     7021 ggtgagatct tgtcttgcga agatgtgcca gaaactcacc ttcatgtttg tcaggttaac
     7081 gttggcgaag aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgcaggcatc
     7141 aaggttatgg tggctcttcc gggagctcgt atcgctgaca attacaaaat caaaaaagga
     7201 aaaatccgtg gcttagagtc acttggaatg atctgttcac ttggtgaatt gggtatttct
     7261 gactcagttg tacctaagaa attcgcagat ggtattcaaa ttttgccaga aaatgccgtt
     7321 ccaggtgagg aagtcttttc atatctagac ttggatgatg aaatcatcga actttctatc
     7381 acaccaaacc gtgcagatgc cctttctatg cgtggagtgg ctcacgaagt ggcagccata
     7441 tatgacaagg cagtcaactt taaagaattt actttgacag aaacaaatga agctgcgaca
     7501 gatgcccttt ctgtgggcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     7561 gacaatgtga ctatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaaggc
     7621 atccgtccca tcaataacgt tgtagacgtg acaaactaca tcctgctcta ctttggtcaa
     7681 cctatgcatg cttttgactt ggacacattt gaagggactg acatccgtgt gcgtgaagcg
     7741 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     7801 ctagtcatca ctgttgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     7861 acagaaatct ctgaaaaatc tagtcatgtt gtccttgaag ctgctgtttt caatggcaaa
     7921 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     7981 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     8041 gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag cgggtgagct tgatacttca
     8101 gatgtagaag tttcttcaac tcttgctgat gttaaccgtg tcctcggaac tgagctgtct
     8161 tatgctgatg tcgaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     8221 agctttacag tcagcgtacc acgtcgtcgt tgggatatca caatcgaagc tgatctcttt
     8281 gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa ccagccttcc aaaagacgat
     8341 ggtacagctg gtgaattgac tgtgacacaa aaactccgcc gtcaagttcg taccattgct
     8401 gaaggagcag gtttgacaga aatcatcacc tatgttctaa caactcctga aaaagcagtt
     8461 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     8521 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     8581 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     8641 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     8701 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taaaggaatc
     8761 ctagaagcgc tctttactcg cttgggacta gaagtgacct atacagcaac agctgagatt
     8821 gccagccttc acccaggacg tacagcagtc atttcactcg gtgaccaagt tcttggtttt
     8881 cttggccaag tgcatccagt cactgctaag gcttacgata ttccagaaac gtatgtggct
     8941 gagctcaacc tttcagtcat cgaagctgcc cttcaacctg ctgctccatt tgtggaaatc
     9001 acgaaattcc cagcagtcag ccgtgacgtt gcccttctcc tcaaggcagc agtaactcac
     9061 caagaagtta tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     9121 tttgacgttt tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     9181 accttccaaa atccagaaga cagcttaacg gacgaagaag tcgcacgcta catggaaaaa
     9241 atccaagcat cgcttgaaaa aaaagtgaac gcagaagtgc gttaaacgat agtaggatac
     9301 aaattccccc tctagtttat gaacactaga ggggtgattt tatataatac tcaatgaaaa
     9361 tcaaaaagca aactaggaag ctagccgtaa gctgtacttg agtactgtaa ggcgaggctg
     9421 acgtggtttg aagagattgt cgaagagtat aaaataatta aaatgtgatt tctgtaaaaa
     9481 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
     9541 ttttaggcaa taaaatatga tctattgttg cattaaaaag ttttggactt tggctattcc
     9601 aagttcctct ttcaaaaggt ggtagtacat ttaatgcgtc cctatgagag cttatttttg
     9661 caagtgctcc atgacgcata gttgcattaa aatcatctgc aataatagcc tttggatatt
     9721 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
     9781 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
     9841 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
     9901 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
     9961 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    10021 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    10081 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    10141 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    10201 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    10261 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaatagc agccatagtt gcagtagcta
    10321 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaaata ttaagattag
    10381 aagatggaaa aaaagaataa gttatgatag ggataacaaa gagaatgacc gaaagggcag
    10441 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    10501 gtgactttgt gactttactt gattgatatt ctttttcttg tcttcctcat agattaagta
    10561 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    10621 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    10681 tggaaaaaag aagggaaatc aaatagagaa attgcctctc t
//
LOCUS       DAVBCR010000067        10613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCR010000067 DAVBCR010000000
VERSION     DAVBCR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.067"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..266
                     /locus_tag="VQ508_001649"
     CDS             <1..266
                     /locus_tag="VQ508_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2490617.1"
                     /translation="SIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGW
                     GDHYQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            720..1898
                     /locus_tag="VQ508_001650"
     CDS             720..1898
                     /locus_tag="VQ508_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490618.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2213..2344
                     /locus_tag="VQ508_001651"
     CDS             2213..2344
                     /locus_tag="VQ508_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2490619.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2365..3915
                     /gene="dltA"
                     /locus_tag="VQ508_001652"
     CDS             2365..3915
                     /gene="dltA"
                     /locus_tag="VQ508_001652"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2490620.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEALVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQSLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3912..5156
                     /gene="dltB"
                     /locus_tag="VQ508_001653"
     CDS             3912..5156
                     /gene="dltB"
                     /locus_tag="VQ508_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2490621.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5170..5409
                     /gene="dltC"
                     /locus_tag="VQ508_001654"
     CDS             5170..5409
                     /gene="dltC"
                     /locus_tag="VQ508_001654"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2490622.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDICVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5402..6670
                     /gene="dltD"
                     /locus_tag="VQ508_001655"
     CDS             5402..6670
                     /gene="dltD"
                     /locus_tag="VQ508_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2490623.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6825..7265
                     /gene="adcR"
                     /locus_tag="VQ508_001656"
     CDS             6825..7265
                     /gene="adcR"
                     /locus_tag="VQ508_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2490624.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7265..7969
                     /locus_tag="VQ508_001657"
     CDS             7265..7969
                     /locus_tag="VQ508_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2490625.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7962..8768
                     /locus_tag="VQ508_001658"
     CDS             7962..8768
                     /locus_tag="VQ508_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2490626.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8778..10283
                     /gene="adcA"
                     /locus_tag="VQ508_001659"
     CDS             8778..10283
                     /gene="adcA"
                     /locus_tag="VQ508_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2490627.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDVEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         3042 a         1984 c         2285 g         3302 t
ORIGIN      
        1 ccagcataga tgagcgcttg cgtactcgcc tacgagtgat tatctggaag caatggaaga
       61 agaaatcgag acgattatgg ggattgctta agttaggagt tcctaaatgg atagcagata
      121 aggtatctgg ctggggcgac cattatcaat tagtagctca gaagtcggta cttaaacgtg
      181 ctatatcaaa accagtcctg gaaaaacgtg gactggtttc gtgtttggat tattaccttg
      241 aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg aacggcacgt acggtggtgt
      301 gagaggggct agagattatc ctctactcga ttaactcccc tgaaatttgt tttaattatg
      361 caaatttcac gtatttttga tgctgagacg acgatcctgg gaacttttca gatatttttt
      421 tgactatcta aaatctatca ttagaaaagc ttagagcgcc aaaggatttg agcgtttttc
      481 tgatttttaa gactttttcc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
      541 taacttctta gtactagcca acaacgataa tcataattcc tcctaaaatt aggaataata
      601 aaggcaatag tttttgtttt ttcatgtaaa aaacctcact tttgttttct gctattttat
      661 gctaaaatat taaaaatcaa atttaattcc aaagtttgta actaaagggg gagcgctaca
      721 tgtctaattc atttgtcaag ttgttagtct ctcaattatt tgcaaattta gcagatattt
      781 tctttagagt aacaatcatt gctaacatat acattatttc aaaatcagta attgccacat
      841 cactagttcc tatcttaata ggaatatcct cttttgttgc gagtctttta gttccgttgg
      901 ttactaaaag gttagcgcta aatagggttt tatctttatc tcaatttgga aagactatat
      961 tattggcgat actggtagga atgtttaccg taatgcaatc cgtagcgcct ttggtgacct
     1021 atctatttgt tgttgcaatt tccatactag atggttttgc agcacccgtt tcctatgcta
     1081 ttgtgccacg ctatgcgacc gatttgggta aggctaattc agccttatca atgactggtg
     1141 aagctgttca attgataggt tggggattag gtggactctt gtttgcaaca attggtctgt
     1201 tacctaccac gtttatcatt ttagtcttgt atatcatttc tagctttctg atgttatttc
     1261 ttcctaacgc tgaagtggag gtgttagagt cagaaactaa tcttgaaatt ttgctcaaag
     1321 gttggaagtt agttgctaga aatcctagat taagactttt tgtatcagca aatttattgg
     1381 aaattttttc aaatacgatt tgggtttctt ccattatact tgtttttgta acggagttat
     1441 taaataaaac ggaaagttac tggggatatt ctaatacagc atactctatt ggtattataa
     1501 ttagtggctt aattgctttt aggctatctg aaaagttcct tgctgctaaa tgggaaagta
     1561 ttctttttcc tcttgtagct atggcaatag tgacactgac tattctatat tttccaaacg
     1621 cacagatgtt tttattattt tcagctttag ttggtatgtt atcgcaacta aaagaagttc
     1681 ctgaaagtgt atttcttcag gaaacagtag aggaaaatca tttagttaat gtctattccg
     1741 ttcttgaagt gatttctaca ttagcatttt cagtatttgt tttgctaatg agctatatta
     1801 ctgagagttt tggtattagc atcagttttt ggctatcagc catttgtctg atgatagaag
     1861 ctattttaat ttatatcaga cgagattatt ttaaatgaga gagtggttaa aaagtcttta
     1921 aaaatcagaa aagcgcatag tatcaggtgt tgaaaaaact tgatattgtg tgttttatta
     1981 tggggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     2041 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     2101 acggtaaaca attgaaaaga tactatataa agtggtacaa tggtagaaaa ataagctagt
     2161 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     2221 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     2281 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     2341 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     2401 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     2461 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2521 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2581 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2641 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2701 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2761 cttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2821 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2881 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2941 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     3001 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     3061 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     3121 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     3181 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcga gcgtttccca
     3241 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct ctcagcagtt
     3301 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     3361 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     3421 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3481 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3541 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3601 ggttaccgca ttgagttaga agatgtctct caaagcctca acaagtctcg ctttatcgaa
     3661 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3721 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3781 atcaaggaag acctgacaga catcatgatg tcctacatga tgccatctaa attcctttac
     3841 cgagacagtt tgccactaac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3901 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3961 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggc ctctttttca
     4021 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     4081 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     4141 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     4201 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     4261 cgcagtcttt gcttgggttc ttgggaattt cttacctgac ctttcgttcg gttgggatta
     4321 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     4381 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     4441 attatcagac cattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4501 atatcatgcg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4561 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4621 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4681 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4741 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4801 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4861 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4921 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4981 atgcctgggt tcgcaagaaa aaaacgctca ataaggaacg gaaaaaagca gggaaggctg
     5041 ccctacctga gaatcgctgg attcagttgc ttggtatggt tgtcactttc catgttgtca
     5101 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     5161 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     5221 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     5281 acggttgagt tgattgtgga gattgagaac cgttttgaca tttgtgtccc tgtaacagag
     5341 tttggtcgtg acgactggaa tacagctaat aaaattatag ctggtattgt ggagctacaa
     5401 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     5461 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5521 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5581 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5641 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5701 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5761 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5821 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5881 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5941 agctatgaag gacctggttc agaagttggc aagtaaagga gaattgtcga cagcagacaa
     6001 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcctcctttt ttggtcagtt
     6061 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     6121 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     6181 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     6241 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     6301 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     6361 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     6421 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6481 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6541 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6601 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6661 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6721 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6781 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6841 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6901 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6961 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     7021 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     7081 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     7141 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     7201 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     7261 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg agcctgttct
     7321 tgaacatatc aattatagtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     7381 agcggctaag acgacgctca tcaaggctag tcttggaatt ctgcaaccac gcattggaaa
     7441 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7501 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7561 tcgctatccg agaaaaggct ggttccgtcg tttgaatgct catgatgagg agcatatcaa
     7621 ggccagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7681 tgggggacaa aagcagcgag cggtgattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7741 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7801 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7861 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7921 gcgttgtttc aacgttcatg agaatggcca ggaggtgggc catgcttagt ttattatctt
     7981 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     8041 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     8101 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     8161 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     8221 tggaaatcgg gacagctatc ctcatgtcaa caggtctggc tgtttctctg attgtcatga
     8281 gcaagggtaa aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     8341 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     8401 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     8461 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8521 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8581 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8641 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8701 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8761 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8821 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8881 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8941 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     9001 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     9061 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     9121 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     9181 atgagtttga tccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     9241 ttcgtgacag cttgtcagca gattatcctg ataaaaaaga gacctttgag aagaatgcag
     9301 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     9361 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     9421 gactcaaaca agtcgcaatc tcaggacttt ctccagatgt agagccatca gctgctcgct
     9481 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9541 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9601 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaat tacatctcta
     9661 tcatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9721 agcctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9781 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9841 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9901 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9961 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatat acttacaagt
    10021 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
    10081 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgac cacaatgttg
    10141 ccccagttaa ggcagaacat ttccatatct tttttggagg tacaagccaa gaagctctat
    10201 ttgaagaaat ggataattgg ccaacctact acccagataa cctatctggc caagaaatcg
    10261 ctcaagaaat gttggcgcat taatgagaaa tcgactagtt cataagagct agtcggtttt
    10321 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
    10381 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
    10441 ccaaagcccc taactagtgc catagttaag ggcttttttg acgtatctta gcgatttaac
    10501 gggtgtttat aggctagtca gtcggtctac ttcttaccat ctagccattt gcagacgaaa
    10561 tacaagataa ttccagctat aacatctgct ataatagtaa gagcaagctc tgg
//
LOCUS       DAVBCR010000068        10603 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCR010000068 DAVBCR010000000
VERSION     DAVBCR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.068"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(6..1220)
                     /locus_tag="VQ508_001660"
     CDS             complement(6..1220)
                     /locus_tag="VQ508_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2490628.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1669)
                     /locus_tag="VQ508_001661"
     CDS             complement(1376..1669)
                     /locus_tag="VQ508_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490629.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1831..2283
                     /locus_tag="VQ508_001662"
     CDS             1831..2283
                     /locus_tag="VQ508_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2490630.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2318..3706)
                     /locus_tag="VQ508_001663"
     CDS             complement(2318..3706)
                     /locus_tag="VQ508_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2490631.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3772..4734
                     /locus_tag="VQ508_001664"
     CDS             3772..4734
                     /locus_tag="VQ508_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2490632.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4731..4922)
                     /locus_tag="VQ508_001665"
     CDS             complement(4731..4922)
                     /locus_tag="VQ508_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490633.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDRTDEVS
                     NHTYGRATLTWFAEIFEEY"
     gene            complement(4986..5996)
                     /gene="ccpA"
                     /locus_tag="VQ508_001666"
     CDS             complement(4986..5996)
                     /gene="ccpA"
                     /locus_tag="VQ508_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2490634.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6421..7020)
                     /locus_tag="VQ508_001667"
     CDS             complement(6421..7020)
                     /locus_tag="VQ508_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490635.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7021..8118)
                     /locus_tag="VQ508_001668"
     CDS             complement(7021..8118)
                     /locus_tag="VQ508_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2490636.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8118..8855)
                     /locus_tag="VQ508_001669"
     CDS             complement(8118..8855)
                     /locus_tag="VQ508_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490637.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8857..9741)
                     /locus_tag="VQ508_001670"
     CDS             complement(8857..9741)
                     /locus_tag="VQ508_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490638.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(9728..9922)
                     /locus_tag="VQ508_001671"
     CDS             complement(9728..9922)
                     /locus_tag="VQ508_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490639.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(9919..10125)
                     /locus_tag="VQ508_001672"
     CDS             complement(9919..10125)
                     /locus_tag="VQ508_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490640.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         3213 a         2411 c         1931 g         3048 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgctaaag gcgaggttaa gaacgtagag atagcaggca ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca
     1441 tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa
     1501 ataagattag atggtagagt acatgaaaga ctaaaaatcc aataaaactt ggataaagaa
     1561 gcggtaccag actctctaat tgacagatca tgatcacttt taccaagact gaaactagga
     1621 taattccata atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcccttcct
     1681 tcacttcttt ccttctatcc tttcttttat tttatcatat atttatatag attttaagcc
     1741 ctaatattct tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat
     1801 tatagtaaga aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc
     1861 gcaatcgatg gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta
     1921 cgaaacgacg ctaaactcac catcgcacat gtcattgaca cacgcgctct ccaaagcgta
     1981 tccacctttg atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa
     2041 gagtacgaaa aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg
     2101 ggaaatccaa agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc
     2161 ctcgttggcg caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa
     2221 tacatactcc gccatgctaa ggtcgatttg ctggttgtga gagaacaaga aaaaacctta
     2281 taatcacaaa gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat
     2341 ggcgttcgta agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat
     2401 atttttcttc ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct
     2461 tgccatctgg aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg
     2521 ggtccacccc catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat
     2581 ccacttgccc tatcaaatct tgctgagcag gtgtcttctt ggctacttta cctgctgcct
     2641 tatcaagggc ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg
     2701 ctgctcgaac aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct
     2761 ctaaatccca agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct
     2821 tgaccttatt gaaatgatag tcacggctga caaagacttt ttctgaagac aaaacaccaa
     2881 aatgttccaa ggccttgtgg atcccatctt gttgattgct agtggtaatg tgcttggcag
     2941 cttccttgac actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca
     3001 tttcaaggtc attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt
     3061 ctttcccaac tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg
     3121 caaaaggatt gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt
     3181 cttctggtgt catcagcatc aaaacttggt agataggctg attcatcagg tgaagcaggt
     3241 cctcttcctt ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc
     3301 gagttaaaac agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg
     3361 acatgatttt agaacccaac atggcatcct tggtccctag ggcaatctcc gtgccctctt
     3421 ttttagcata gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga
     3481 ctctgtcttt actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat
     3541 cgtccatcaa ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc
     3601 cttgttcttt gacaatctta atcgcgtcct tagtggattt caaaacactc ttgcgattgt
     3661 tgaccaaggt tccatcgata tcaaaaaaaa cagctttgac ttccatccta ttccaatctc
     3721 cccttttgtg atacaatgat tataccacat ttcagaaaga gtgagtaaat catgcctaag
     3781 aaaatccttg ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct
     3841 gttgtgacga gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa
     3901 gtccacgcct tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg
     3961 gtcctctacc agaaaattaa agaggaagca gataactacg atggagtggt aatcacacac
     4021 ggaaccgata ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg
     4081 cctatcgttc taacaggagc catgcgtagc tccaatgagc tcggtagtga tggtgtttat
     4141 aattacctaa gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg
     4201 gtcgttatga acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat
     4261 gtcagcacct tccagactcc aacacatggc cccctcggtc tcatcatgaa acaggaaatc
     4321 ctctacttca aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta
     4381 gtccctatca tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta
     4441 gaacacttgg acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg
     4501 gctcaaaaat tagaaagcct tctgcaaaaa ggaatcccag tcgctctggt atcacgatgc
     4561 tttaacggta ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa
     4621 gcaggcgttt tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc
     4681 gccctcaatg ccggactaac aggacaggct ttgaaagact atatggaagg ctaatactct
     4741 tcgaaaatct ctgcaaacca cgtcagagtc gccctaccgt atgtatggtt actgacttcg
     4801 tcagttctat ctacaacctc aaaaacatgt tttgagctga cttcgtcagt ttcatctaca
     4861 acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc tatggtattc
     4921 attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg attccctgat
     4981 tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg taagagaact
     5041 tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat actaatggca
     5101 ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa gcgtgagatt
     5161 tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacacc cttatcagcc
     5221 aaaccgttca agactcctgc tgccaactca tcacctgtca caactgctgc agttgcattt
     5281 gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt agattcaaat
     5341 accaaaccct cactataagt gattcctact tttttcaagg tttccttgta gccaactaaa
     5401 cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat acgctcattt
     5461 tctttagcaa ggtagctcac tgcatcaatt gttgcttgct tatagtcaat attgacactt
     5521 ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg tgaacgcgaa
     5581 aattctgagc gaattttatc tgtcaagtga taacccatat agataatgcc atctacctgc
     5641 tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc gctattagct
     5701 aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc caaactcgaa
     5761 aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt tttacttgca
     5821 agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc tagcactttt
     5881 ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac cgtcgccatg
     5941 gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc attcattcct
     6001 tttcctgtcc tttctatctc acacattctt ttacaagtag aggtactgat tgaagctcta
     6061 tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac ttattccatt
     6121 ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa aaatttcata
     6181 gaaaacctag gtttagctcc ttgctaccac cttagactaa ataaaaagga ggaaactaag
     6241 cccttctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag gacagtcaaa
     6301 tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatag
     6361 gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa tattatatca
     6421 tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac cagcatccag
     6481 ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt aaagctgatc
     6541 tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa tctcttgttc
     6601 tgtcaacgga ttgggacggg tcatcaccac ttccatcaat tcaggcgaat actccttgcg
     6661 tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt cctttaatac
     6721 ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct tgaaggtcgt
     6781 caccacaacc acctttgttt caagcctttc tgatcgtatc cactccaaga cttcaagacc
     6841 tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct tttctaatag
     6901 ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg gttgaagcgt
     6961 gagcaattga cacatagcat ctcgcaacat actttgatct tctgcgacta atactttcat
     7021 ctactttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt tctgactgat
     7081 tacacttact tctcctgaaa atggaaggac acgttctcga actgtatgga gctcattttc
     7141 tgttacagtt tcaaaacctt tcccgtcatc tctgaccatc aggagcaatt cctgatctgt
     7201 gcgttctaat tttaagtagg ctttagacgc tttagcatgt ttgatgatgt tggtcactaa
     7261 ttcaagcaaa atcatggaag ccgttgactc taattcctga gttaagctag ctgtatctag
     7321 ttgattagct atttccacct caattccagc aatttctaac atctttttca cagtctctag
     7381 ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgcactt cacgcattga
     7441 ttctttgcta atctgctgta tttctcttaa ttccttttcc acttgtggat aagcctgtat
     7501 ttgaaataac tgcaaggcta gatcagtttt aacactaagc atagcaaagg tatgccccag
     7561 actatcatgc aaatcttgac cgatacgatt gcgctcattt tctgctagca atagatttat
     7621 ctgggtattt tgcttagtct gagcttcttt caaatcctcg acaaggcgaa ttcgaaccga
     7681 gcccaaagtc atcaaatcaa caaaagtaag aattccaagc agatagacta gaaactcaac
     7741 ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg acttgagcaa gaagaaaagt
     7801 ccagacatgt aaagacttta aactacgtac gctgaaatga taacttaaga gattggaaag
     7861 gaaaaagaaa aaccagatat aattcacagc aacaaaggcg gtattcccag ctacatagat
     7921 cagcatgatc ccccagaaaa gccaagatag gcgctggctc ttagttgtta aaacacccaa
     7981 atatgccact acaaatagaa tatcaatcaa taaatgccag gcagaaagcc acccagtcac
     8041 tacaggtagg atggggaaaa tcataaaaat taaactggcc caaaacatat agtgtatgct
     8101 tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat ggtcaaacca
     8161 aacaaaactg ctgaaaaaac aagtagataa actgtggctg atagattgat gctaccctcg
     8221 tttaagaagg tcttgagcaa ctccatcaac tgataagttg gtaggcactt cccgattgct
     8281 tgcatccagt ctggaaataa agagatgggc atccagagtc cacctaaaac agccaagcct
     8341 agatagagaa gattgcccac gacagacatc agctgactag aaggcaagag tgtcaaggtc
     8401 aagccaagcg ctacaaaagc tatacttcct actatcagca agaacgcagc cccaatccag
     8461 cttcctaaag acatatccac acctctgacc aagtgcccaa ctgagaaaac aaccaggatt
     8521 gagaccaaat aatcaaccat catacttgtt atctttgata gataatattc taccatattt
     8581 acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg taagacagct
     8641 gggaatgaaa agatagcggt cgacatcatg gaaaaggcag tcatggagat gaggtagtcg
     8701 cgcataaaat tagccggtcc acctggtgtg tcctggtaca tgcctgaaaa gaatagatag
     8761 aaggctgtcg gcatccctac agataataaa taatagactg attgccgttt ggtcaataga
     8821 aattctatct tgtttagtgc aatccatcgt ttcatcttag tcatctccct tctgtgtttc
     8881 ttcaaagatt gtatccaaca aactacgatt attgacttcg atttcttgta tcctacatcc
     8941 tgcttgaact aacagttccc agaaagcatc cgcttcgcat gtgactactt gtaaggcatc
     9001 ttgtttttgt acccagtttt caaccaagac tgacttctca acgacttcct tgtaagctag
     9061 cggaaggata aaatgctttt caatctcctc actacgcata gctagaggcg tcgtgtcacg
     9121 aatcaactct cccttattta aaaccaaaat ccggtcagct gtatgctcta cctcttcaat
     9181 ataatgagac gaatagagaa tggtgactcc ttgcgctttt aggtcccgaa caatttccca
     9241 aaaacgttga cgagttgaag tatccatggc agcagttggc tcatctaaaa agacaagctt
     9301 tggtcgccca attaaggtca agataaaaga taaaagacgc ttttgcccac ctgacaattt
     9361 ttctgcgaat tgctctttct gttgctggtc aaactgtaat agttgatcga tttcctggtt
     9421 gttcaaggga tttggataga tacgttgaaa gaaagcaatc aactctttaa cctttagttt
     9481 ttgaacaatt acattttctt gagggagata ggatctcgta tagtctaact tagagctcgt
     9541 taccggcaaa tcttggatat atacttgccc actggtgacc aatttatctc caagcaaaca
     9601 gtccaagagt gtcgtcttac cagctccatt tggcccaatc aaggcgacgc attcaccttc
     9661 agctacctca aaggaaatat ccttcaaaat agccttactt ttgatgtttt tatgtaagcc
     9721 ttgtacctta atcatgttca taatattctc ctttcaacca ctccattccc atagggaaaa
     9781 cgacggaaat cataaatcct aatccccagg cgccgcgaat aacttggtga agaaaggatt
     9841 gatcaaacca acctgtaaac atttctacca accataccac tagtgatagt ccaataaaaa
     9901 gaaagagaat cgcttttttc atttttcaag ctcctttttc acatctctta ctaatttcaa
     9961 gccttctcgt atcaaccaag acatgattcc aaagcttgca aacaactccc aaggaaaatg
    10021 atagaatcct tcgtccaatc ctgagaacat gagataagtc atgactcctg ctactactaa
    10081 actcactgcg ataatcattt tatttctcat ctcttcttcc tccatttcat actacaatta
    10141 tagtcttttg aaatcagagg agatagaagc ttctgtcact aaaaaatatg acaaatgtca
    10201 taaaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa ttagataaat
    10261 ctaaggcaac ttcctcaaaa gagatatcaa accccattca cactataatg taaactaata
    10321 cttatttaaa gtcaaaagag cggaaatttt tatcgtacaa acatatatat tataggtaag
    10381 acaaaaaact cttcaattac tttcttaaaa atttatagga gttataaaat ctattaaatt
    10441 tcaatgtgga tacctaaact acagtattta ttgaaaagac tggagacaaa aagtatacgc
    10501 tgccaaaatg aattgctgaa aatcaaaaaa gagagaagca aactgattct ctcttaatgt
    10561 atataatatc tagttataaa aatacacact cacatatctc tgt
//
LOCUS       DAVBCR010000069        10525 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCR010000069 DAVBCR010000000
VERSION     DAVBCR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.069"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(80..1033)
                     /locus_tag="VQ508_001673"
     CDS             complement(80..1033)
                     /locus_tag="VQ508_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2490641.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(1101..2310)
                     /gene="sstT"
                     /locus_tag="VQ508_001674"
                     /pseudo
     CDS             complement(1101..2310)
                     /gene="sstT"
                     /locus_tag="VQ508_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            2449..3009
                     /locus_tag="VQ508_001675"
     CDS             2449..3009
                     /locus_tag="VQ508_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2490642.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            3066..3974
                     /locus_tag="VQ508_001676"
     CDS             3066..3974
                     /locus_tag="VQ508_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2490643.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            3926..4516
                     /locus_tag="VQ508_001677"
     CDS             3926..4516
                     /locus_tag="VQ508_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2490644.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            4519..5625
                     /gene="yqeH"
                     /locus_tag="VQ508_001678"
     CDS             4519..5625
                     /gene="yqeH"
                     /locus_tag="VQ508_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2490645.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            5839..6150
                     /gene="yhbY"
                     /locus_tag="VQ508_001679"
     CDS             5839..6150
                     /gene="yhbY"
                     /locus_tag="VQ508_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2490646.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            6202..6831
                     /locus_tag="VQ508_001680"
     CDS             6202..6831
                     /locus_tag="VQ508_001680"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2490647.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            6831..7424
                     /gene="yqeK"
                     /locus_tag="VQ508_001681"
     CDS             6831..7424
                     /gene="yqeK"
                     /locus_tag="VQ508_001681"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2490648.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            7425..7925
                     /locus_tag="VQ508_001682"
     CDS             7425..7925
                     /locus_tag="VQ508_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2490649.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            7938..8291
                     /gene="rsfS"
                     /locus_tag="VQ508_001683"
     CDS             7938..8291
                     /gene="rsfS"
                     /locus_tag="VQ508_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2490650.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            8611..9351
                     /locus_tag="VQ508_001684"
     CDS             8611..9351
                     /locus_tag="VQ508_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2490651.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            9361..10458
                     /locus_tag="VQ508_001685"
     CDS             9361..10458
                     /locus_tag="VQ508_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2490652.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3164 a         2071 c         2446 g         2844 t
ORIGIN      
        1 cgttttgagg ttgtggatag aactgacgaa gtcagtaaca tatatacagc aaggcgacgc
       61 tgacgtggtt tgaagagtat tactgtctat atttttggta aaaatcaact tttacttgga
      121 tgaaggtttt ggcttcacgt aggagttgaa gaagggtggc gcgggtttca aattcttctc
      181 ttgtcttggg cagactgcgg ttccggaaga cttccagata acgttcaatt tcatctagca
      241 aatcagaagc aggattggtc tggctcagtt gacctgcaat ttttgaaaag agttgcgcta
      301 agatcaggct ttcactggcg gcaaggtgac aagtgttaat ctgttgggcc atgtttctca
      361 ggatacgact ttgtcgttgt ctcatctcaa agtagtggat atggtagtcg gtctggtgaa
      421 agaggtggtc agagtgatcc aaatagacca gtctgagggc ttctttcaaa agcgtgtcta
      481 attctgctac cagctgtgct cggttgcgtc cgtctcctct ggataaatag tatttgaagc
      541 gctggaggat atcttttaac ttttcttcca ccagcgtgtg gtagtgctgg atttcctctt
      601 ctcgtgaagg catatagaga ttaacaagca aggcaaatcc tgtaccgata gcaaagagaa
      661 ggaattcgtt gactaggagg tctggagagg ttgactcttg aaccaagaga tggctaacca
      721 aaaccgtgct tggtgtgatg ccaatttccc agcccatctt gtaggctaaa ggaacgtaga
      781 aggccagata gaggccgaga ctccagatat gaaatccgct caagtgaaaa gctagaacac
      841 cgatagccag agctagaagc atagaaaaaa gacgattgcg agccagtttt aaagtacttc
      901 tacgcgtatc agataggctc aagagagcga taattccagc cgaaactgct gacgaaagat
      961 tgagaaaata agcaaacagg caggcaagac aggtagctaa gatgagcttg gtcgtacgtt
     1021 ggctaataga cataagaatt tcctaataag ttagaataaa agcgtaaaag acaagacatg
     1081 agcaggcttg ccttgatgag ttatttttta cgggttgctg cgtattcggc aacggcggta
     1141 aagaggacat ctgtagaaga gttaagggct gtttcacatg agtcttggat gacaccaatc
     1201 acaaaaccaa ccccaacaat ttgtatggca atatcgttag aaataccgaa aaggctacaa
     1261 gcaactggga taagaaggag ggaacctccg gcaatacctg aagcaccaca ggctgagata
     1321 gctgctacca cactgaggac aaaggctgtg gcaaagtcaa caggaattcc aagagtgtta
     1381 actgtaacaa gggtcaaaag gttaatggta atcgctactc cagccatatt gatagtagaa
     1441 ccgagtggga tagaaacaga ataggtatct gggttgagtc caaggtcatg gcagagtttc
     1501 atattgacag gaatgttagc cgcagaacta cgagtgaaaa aggctgtcac accgctgaca
     1561 cggaggcagt tccaaactag agggtaagga ttgcgtctca taaagaagaa ggcaatcaaa
     1621 gggttgacca caggggcaac aaaaagcata gtcgttacta atagaaccaa taaaataccg
     1681 tagttggcaa gccttccgac tcccttgtca gaaatggttt taaaaacaag accaaggatt
     1741 ccaaatggag tcagattgat gatccattcg acaattttag aagtcacgtc agcgatagtt
     1801 tttagcaatt ctttactatt tttactggct tctctcatag cgattccaaa aatgactgcc
     1861 caagataaga ttccaatata gttagcagta agcagggcgt tgactgggct gtcaaccagt
     1921 ttgagcaaga ggttgctgag gacctgccca atcccatctg gtggtgcaat ttcagtattg
     1981 gcactattta gggtaatttc aatagggacg atgaaacttg ctagtacagc tacaagagca
     2041 gcggcgaaag tccctatcat aggatataca agaaaacaac agttttcata ttgctatctt
     2101 gtcccttttg atgttgggaa agggcattgg caacgagagc aaagactagg ataggagcaa
     2161 cagcttttag acctccaacg aataaatccc cgagtagccc aatccctgag agattaggaa
     2221 gggtcagtcc taggattccc cacaaagcat accaatcaag atacgcttga caaggcttgc
     2281 cttattccaa gcatgaatga ttcttttcat aataatctcc tttttgtgta gtgattatga
     2341 ttatagtata aatgatagac aaaatcaaga attttctgtc tattttttga atatttatgg
     2401 agaatgagac tgatgaaaat atggtataat gaaataaagg agttttatat gcaaaaattt
     2461 attcaggctt atattgaaaa gctagatgtg acaaccatta tcgagaatat tctaaccaag
     2521 gtcatttctc ttttactgct tttgattgta ttttatattg ctaaaaaaat gcttcatacc
     2581 atggtgcaga gaattgtcaa accttctcta aaaatgtctc gtcatgatgt cggacgccaa
     2641 aaaaccatct cacgtttact agaaaatgtg tttaattata cgctatattt ctttttactc
     2701 tactgcattt tgtcgatttt aggtttgcca gtttctagtt tgctggctgg agctggtatt
     2761 gctggggtag cgattggtat gggagcccaa ggctttctgt ctgatgtcat caatggcttt
     2821 ttcatcctct ttgaacgtca actggatgtg ggagatgagg tcgttctgac aaatggaccg
     2881 attactgtat cgggtaaggt tgtcagtgtg ggaattcgta cgacacagct tcgtagcgag
     2941 gagcaagccc ttcactttgt ccctaaccga aatatcacag ttgttagcaa tttctcacgc
     3001 acagactaga cctgttattt taagtaattt gtggtacaat agagggagtt taataaggag
     3061 aaaagatggt tttagaaaag cagttgggcc atggttgtac ctggatagac ctagacctag
     3121 gaaagttgaa taaactagaa gacctttctg aaatttacgg tttggacaag gaaaccattg
     3181 aatacgcact ggatagaaac gagcgcgccc acatggacta ccaccgtgaa agtgggacgg
     3241 ttacctttat ctataatgtc ctagacgtaa aaaaggacaa ggcctactat gagacttttc
     3301 ccatgacctt tatcgtcgag catcgtcgcc tgattaccat tagtaatacc aagaacgcct
     3361 atgtcattga acagatgact cgttatctgg agagccatga cacgctttcg atttataagt
     3421 ttctctttgc cagtctggaa atcatcagca atgcctacta tcctgtcatt gagcagatgg
     3481 acaagagtag ggatgaggtc aatgacctct tgcgccagcg aactaccaag aaaaacctct
     3541 ttgccctgtc tgatttggag actggtatgg tttatctgac ggcagctgcc aaacaaaatc
     3601 ggattttgtt agagcatatt caaggtcatg ccttgtatcg tagttttgat gagattgaga
     3661 gagaacagtt tgatgatgcc atgattgagg ctcatcagct ggtatccatg acagacctaa
     3721 tctctcagat tttacagcag ctttcagcct cttacaacaa tattctaaac aataatctga
     3781 atgacaattt gacaaccttg actatcattt cagtcttgct agctgttttg gcagtcgtga
     3841 caggcttttt cggaatgaat gttcccttac ctttaacaga tgagccccat gcttggctct
     3901 atatcagttt ggctagtgca ggtttgtgga ttgttttatc cttgttacta aggaaaattg
     3961 cgaaaaaaag ttaagaaaag gagccagaat ggcgattgaa aattatatac cagattttgc
     4021 tgtggaagca gtctatgatc tgacagtccc aagcctgcag gcgcagggaa tcaaggctgt
     4081 tttggtcgat ttggataata ccctcattgc ttggaacaac cctgatggaa cgccagagat
     4141 gaagcaatgg ctacatgacc ttcgggacgc gggtattggc attatcgtag tgtcaaataa
     4201 caccaaaaaa cgcgttcaac gagcagttga gaaatttggg attgattacg tttactgggc
     4261 cttgaagccc ttcacatttg gtattgaccg tgccatgaag gaattccact atgacaaaaa
     4321 ggaagtggtc atggttggcg accagctcat gacagatata cgagcagccc accgtgcagg
     4381 gattcggtca attttagtca aacccttggt ccaacatgac tcaatcaaaa cgcagattaa
     4441 ccgaactcgt gagcgtcgtg ttatgagaaa aatcactgaa aagtacggac cgattacata
     4501 taaaaaagga atttaactat ggaagaaatt ctctgtattg gttgtggagc aaccattcag
     4561 acgacagata aagctggtct tggttttacc ccccagtcgg cacttgaaaa aggtttggag
     4621 actggcgaag tctattgcca acgctgtttc cgtctccgcc actacaatga aatcacagat
     4681 gtccagttga cgaacgatga tttcctcaag ctcttgcacg aggtgggaga cagtgatgcc
     4741 ttagtggtca atgtcattga tatctttgat tttaatggat ctgtcatccc aggtttacca
     4801 cgtttcgtct cgggcaatga tgtcctcttg gtaggaaata aaaaagatat ccttcctaag
     4861 tcagttaagt ctggtaagat tagccagtgg ctcatgaaac gtgcccatga agaaggtctt
     4921 cgtccagtcg atgtggtcct aacttcagca caaaataaac atgccattaa ggaagtcatt
     4981 gataagattg aacactaccg taagggccgc gatgtctatg tggtcggtgt gaccaacgtt
     5041 ggaaaatcaa ctctaatcaa tgctattatc caagaaatca cgggtgatca gaatgtcatc
     5101 actacttcac gcttcccagg gacaaccttg gacaaaatag agattccgct tgacgacgga
     5161 tcttatattt acgatacgcc gggaattatc caccgccacc agatggctca ctacttgacg
     5221 gccaaaaacc tcaagtatgt cagtcctaaa aaggaaatca agcctaagac ctatcagctt
     5281 aatcctgagc aaaccctatt tttaggtggt ttgggacgct ttgactttat agcaggagaa
     5341 aagcaaggat ttactgcttt ctttgataat gaactcaaac tccatcgtag caagcttgaa
     5401 ggagctagtg ctttctacga taagcacctg ggaactcttc tgacaccacc aaatagcaag
     5461 gaaaaagaag atttcccaag gctagtccag catgtcttta ccattaaaga taagacagac
     5521 ctagtcattt caggcctagg ctggattcgt gtaacaggca cagcaaaagt cgccgtctgg
     5581 gcaccagaag gcgtcgccgt cgccacacga aaagcaatta tttaaaacaa aatcgctgag
     5641 cgaacaaagt gagctcatat agcgatagtt attgccgtgg tgtgattgcc aatctgtaga
     5701 ttggcaaggg gcacaattga gaccaaggct caattgtgag gtcaggaaat ccatttttca
     5761 aagatgaaat ctttggaaaa ttagttccga ccgtccctca ccacctaaaa taactatcaa
     5821 aaaacaagga agaaaattat gtcattagca tcaaaacaac gtgccttcct caacagccag
     5881 gcacacaccc tcaaacctat catccaaatc gggaaaaatg gactcaacga ccaaatcaaa
     5941 accagcgtcc gtcaagctct tgatgcgcgt gaattaatca aggttactct cttacaaaac
     6001 acagatgaaa acatccacga agtagctgaa attttggaag aagaaatcgg tgtggataca
     6061 gtccaaaaaa taggacgcat cttgattttg tttaagcaat ctagcaagaa agaaaatcgc
     6121 aagatttcta agaaagtcaa agaaatctaa gaccgaaact ccaaataact gtttttatag
     6181 agaaataaag gggactagcc tatgacaatc gaactattga ctccctttac caaggtagag
     6241 ttggagccag aaatcaagga gaaaaaacgc aaacaagttg ggattttagg ggggaatttt
     6301 aaccctgttc acaatgccca tctcattgtt gcggatcaag tacggcaaca gttgggactg
     6361 gatcaagttc ttctcatgcc tgaataccaa cctcctcacg ttgataaaaa ggaaaccatc
     6421 cctgaacacc atcgtctcaa gatgcttgag ttggcaattg agggaattga cggcctagtc
     6481 attgaaacca ttgagttgga gcgcaagggt atttcctaca cctacgacac catgaagatt
     6541 ttgacagaga agaatccaga tacggattat tactttatca tcggtgccga catggttgac
     6601 tatctgccta agtggtaccg aattgatgaa ctggttgaca tggttcagtt tgtgggggtt
     6661 cagcgtccac gctacaaggt agggacttcc tatccagtta tctgggtgga cgtaccgctc
     6721 atggatatct cgtccagcat ggtgcgggac ttccttgccc aaggtcggaa acccaacttt
     6781 ctcctacctc agccagtgct agactacatc gagaaggagg ggctctactg atgtcctatc
     6841 aagactatat caactgctcc cgtgaggcct tgttggaaaa aatggcagag tttctacctg
     6901 agaaacgtct aacccattgt ctaggtgtgg agcgtgccgc tatggaatta gctcagcgct
     6961 ttggtgtaga tgttgagaaa gcaagtctag caggtcttct tcatgactat gctaaaaagt
     7021 tgtcaaatca ggaatttcta gccttgattg accgttacca gctagaccct gacctcaaaa
     7081 actggggcaa taatgtctgg catggtatgg tcggaattta caaaattcag gaagatttgg
     7141 atttgcatga ttcagaaatc ctgcgagcca ttgaagtcca tacagtcggt gctggtcaaa
     7201 tgacagacct agataaggtc atctacgtcg cagactatat cgagcacaat cgagcctttc
     7261 ctggagtaga tgtggcgcgt gagattgcaa atctatcgct caataaggcg gtggcctacg
     7321 aaacagctcg taccgtggag tatctagctc atcagggatt ccccatctat ccccaaaccc
     7381 ttgaaaccta taacgccttt gtgcactatt tgaaagagaa ctaaatgaag acggctttta
     7441 taatcattga tgttcagaat attttagtag aaactgggtt ccagacaaaa agtctattgg
     7501 gaaaaatttc ttatttacaa aaccaggcta gaagcaagaa tattgaaatt atctatgttc
     7561 aacatattga gaactctgaa gctcaaacat cagaagattg gcagttatct gcgcttttaa
     7621 atcgaaaacc tgctgaaaag gtctttcaga agaagtataa cagtattttc aaagaaactg
     7681 gcttaaaaga atacttggat aagcagggga ttgaaaaatt agttttatgt ggtatgcaga
     7741 cagaatattg tgtggatacc tctgtcaagg ttgcctttga atatggctat cagcttattg
     7801 ttccagaagg tgctgtcaca acctttgatg gggatgacat tccagcagaa acgattaatg
     7861 aattttatga ggacatttgg gaggagcgct ttgcagatgt tctagattac aaacatattt
     7921 tctaaaaaga ggattaaatg aacgaaaaag aattactaga actagtcgtg aaagcggctg
     7981 atgagaaacg tgcggaggat atcctcgcac ttgatgtaca agatttgact agtgtgacgg
     8041 actactttgt catcactagc tcaatgaata gccgtcagtt ggacgctatc gcagctaata
     8101 tccgtgaaaa agtagctcaa gcaggcttta aaggtagcca tgtcgaaggc gatgcagctg
     8161 gaggctgggt cttactggac cttggtgctg ttgtcgtgca tatcttttca gaagaaatgc
     8221 gtgcccatta taatctagag aagctatggc atgaggcgaa ttcagtagat atttcagaag
     8281 ctcttgctta gaagatgaac tcagttgtag tcaactgggt ttctttgctt attttagaaa
     8341 aattgataga aaggtaaagg gcctcgtgtt tgtttcattt cgaaactgaa cacgagctaa
     8401 aagctgagat aaaaagtaac tttctttgta tctgacgata gggaagaaag tttcctatgt
     8461 actctttgct tttaaggctc tttgtatctt agaaaggatt tgaacccgtc ctgtaattct
     8521 cggaaaatag atagccttcc ttggagctcg gctccatcgt aaggctccta attttctatc
     8581 gaattacggg cggacttggt atcatgtttt atggcgactt atgaaacctt tgcggcggtc
     8641 tatgatgcgg tcatggacga tagtttatac gacaaatgga cgaatttttc tctgcgtcat
     8701 ttgcctaaga ccaaggagag aaagaaactc ttggaattgg cttgtgggac aggaattcaa
     8761 tcagtgcgct tttctcaggc tggttttgat gtgactggac ttgacttgag tgcggatatg
     8821 ttgaagattg cggagaagag agcaacttca gccaagcaaa agattgcttt tatagaaggt
     8881 aatatgctga atttgtccaa ggcagggaaa tacgattttg tcacgtgtta ttcggactct
     8941 atctgctata tgcaggatga ggtggaagta ggggacgtct ttaaggacgt gtacaatgcg
     9001 ctgaatgaag agggagtttt catctttgac gtgcactcga cctaccagac agatgaagta
     9061 ttccctggct attcctacca tgaaaatgcg gaagattttg ccatgctttg ggatacatat
     9121 gaggacgaag ttcctcactc catcgtgcat gagctgactt tctttatcaa ggaggctgac
     9181 ggttccttta gtcgccacga tgaagtacat gaggagagga cctatgagat cttgacctat
     9241 gatatcttgc tggaacaggc tggatttaag tccttcaaac tctatgcaga ctttgaggat
     9301 aaggagccaa cagaaactag cacccgttgg ttttttgtgg cgcagaagta ggagattagc
     9361 atgaccataa caggtattat cgcggagttc aatccttttc ataatggcca taaatacctg
     9421 ctggatcagg cagagggact gaaaattgtt gccatgtcag ggaatttcat gcagcgtgga
     9481 gagcctgcta ttgtggataa gtggacaagg gcccagatgg cgctggaaaa tggcgcagac
     9541 ttggtagtgg aattgccctt tttagtcagt gttcaggcag ctgatttctt tggtcaagga
     9601 gctgtggata tcttggatcg cttgggcatt gatagtctag tttttgggac agaggaagtt
     9661 ctggattacc agaaaatcgc tgacttatac acagagaaag gtgctgagat ggagaaattt
     9721 gtgaaaaatc tgcctgattc cctttcctat ccccagaaaa cccaagctat gtggaaggaa
     9781 tttgctggtc ttgatttttc aggtaatacc cccaatcatg ttcttgctct ggcctatgcc
     9841 aaggcggttg caggacgaaa tatcaagctg catccgattc agcgtcaggg ggcaggttac
     9901 cattctgtga ataaagatgt ggactttgcc tcggcgacag ccctccgtca acatcagaag
     9961 gaccaagatt tcttagaacg ttttatgcct tctgttgccc tatttgagca ggccagtaag
    10021 gtgatctggg aagactattt tcccttgctc cgctatcaaa tcttgtcaaa tcccgaccta
    10081 accaccatct atcaggtcaa tcaagaaatg gctgtgcgca ttaaagaagc tattaaaaca
    10141 gcccagtctg tggaagaatt ggtcgagtta gtgactacca aacgttacac caaggcgcgt
    10201 gtcagacgcc tattgaccta tattttggta caggctagag aaaatgtctt gccagaagcc
    10261 attcatgtcc ttggatttac tgaaaaagtc aggcaacatc tcaagtctct gaaaggacag
    10321 gtcaatctag tcagccgaat tggcaaagaa ccttgggatg ctatgaccca aaaagcagac
    10381 cagatttacc aactagggaa gccaagtata gcagagcaaa attttggaag agtgccgatt
    10441 agaatagaaa caaactaagt ctaccgaaag gtaggctttt tgagattttt tacgaataat
    10501 actcttcgaa aatcaaattc aaatc
//
LOCUS       DAVBCR010000070        10129 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCR010000070 DAVBCR010000000
VERSION     DAVBCR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.070"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            126..1568
                     /gene="gtfA"
                     /locus_tag="VQ508_001686"
     CDS             126..1568
                     /gene="gtfA"
                     /locus_tag="VQ508_001686"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2490653.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            1706..2899
                     /locus_tag="VQ508_001687"
     CDS             1706..2899
                     /locus_tag="VQ508_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2490654.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3555..5534
                     /locus_tag="VQ508_001688"
     CDS             3555..5534
                     /locus_tag="VQ508_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2490655.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5601..7097
                     /locus_tag="VQ508_001689"
     CDS             5601..7097
                     /locus_tag="VQ508_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490656.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7097..8023
                     /locus_tag="VQ508_001690"
     CDS             7097..8023
                     /locus_tag="VQ508_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490657.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8032..9099
                     /locus_tag="VQ508_001691"
     CDS             8032..9099
                     /locus_tag="VQ508_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490658.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9110..10036
                     /locus_tag="VQ508_001692"
     CDS             9110..10036
                     /locus_tag="VQ508_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490659.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         2982 a         1798 c         2123 g         3226 t
ORIGIN      
        1 aaccatatct acggtaaggc gacactgacg tggtttgaag agattttcga agagtataac
       61 atgaatttga tttacgaagc cagatcatat gagacttggc ttcaattaga aaaaggagag
      121 taatgatgcc aattcaaaat aaaaccatgt tgattaccta ttctgatagc cttggaaata
      181 atcttaaaga cttatatgat aatttggaag agcattttgg agatgctatt ggaggagttc
      241 accttttacc atttttccca tcaacaggtg atcgtggatt tgcgccagtt gactacgacg
      301 aagtggactc agcttttggt gattgggagg atgttaagcg tttaggtgag aaatattatc
      361 ttatgtttga ttttatgatt aatcatattt ctcgtcaatc caagtattat aaggactatc
      421 aagaaaaaca tgaagccagt gaatttaaag atctcttttt aaactgggat aagttttggc
      481 cagaaaaccg tccgacacag tctgatgtag atttaattta caagcgtaag gatcgtgcac
      541 caaagcaaga gattgtgttt gaagatggtt cagtggaaca tttgtggaat acctttggtg
      601 aggagcagat tgatcttgat gtgaccaaag aagtaactat ggaatttatc cgtaagacca
      661 ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct agatgccttt gcttatgcag
      721 tgaagaaatt ggatactaat gatttctttg tggaaccaga tatttgggat ttattggaca
      781 aagttcgaga tatcgctgct gagtatggga cagagatttt acctgagatt catgaacact
      841 attcgattca gtttaaaata gcagaccatg attactatgt ttatgatttt actcttccaa
      901 tggtgacact ttatactctt tacagttcca gaacagagcg tttggctaag tggttgaaga
      961 tgagcccgat gaagcaattt acgacgctag atacccatga tgggattgga gtagtagatg
     1021 tcaaggatat cctgaccgat gaggagattg actatgcttc aaatgaactc tataaggttg
     1081 gagccaatgt caaacgtaag tactctagtg ccgagtataa caacttagat atctaccaaa
     1141 tcaattcaac ctactattca gcgcttggag atgatgatgt caagtatttt ctcgctcgtc
     1201 taattcaagc ttttgcccca ggtattcctc aggtttacta tgtgggtcta ttagcaggca
     1261 agaatgactt gaaattatta gaagaaacta aagaaggtcg aaatattaat cgtcattact
     1321 atagcaacga ggaaatagca agagaagtgc aacgacctgt tgtgaaggcc cttctcaatc
     1381 tattttcttt ccgtaaccgt tcagaagcct ttgatctaga agggactact gagatagaga
     1441 caccaacagc tcacagcatt gtaatcaaac gtcaaaataa agataagtcc gtaacagcag
     1501 tagcagaaat tgatttgcaa aatcagactt atcgggtaat tgagaatgga gttgaagtat
     1561 cattttgaag ccttgatatg gtggataaaa tagggttttt attttggaaa acgtttcctt
     1621 tgttttcaaa ttgctaaaaa agtggtacaa tataggatag cttactatta tctgaatcag
     1681 ctgatttgga gagaaaggat tcattttgaa atcaataggc tttattgaaa agctgaaggg
     1741 gttgtctagt aaagagctga ttttattggg aattatccta agtatctttt taccctttta
     1801 tctttttgta gttgtactct gtttatatat tatcagtttg atttttacag gagacatgaa
     1861 aagtattctt cagaaaatgg gggagcatcc gatgctgctt ctttttctta gctatagtac
     1921 tgttatatcc attcttgcac aaaattggat gggtcttgtg gcttcagtag gaatgtttct
     1981 atttactatt ttctttttgc actatcagtc gattttatcc cataaattct ttcgattgat
     2041 tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct tttgccagtt tagaacattt
     2101 ccaaattgtg aagaaattta actatgcttt tctttcaccc aatatgcagg tgtggcatca
     2161 gaaccgggca gaagtgacct tctttaatcc taattattat ggaattattt gttgtttctg
     2221 tattatgatt gctttctatc tgtttacaac gaccaagttg aattggttga aagtattctg
     2281 tgtgattgca ggctttgtta atctctttgg tttgaacttt actcaaaatc gaactgcctt
     2341 tcctgctatt atcgctggag caattatcta tctctttacg actattaaaa actggaaggc
     2401 cttttggctt agtattgggg tcttcgcgat tggtttgagt ttcctctttt ctagtgattt
     2461 gggagttcga atgggtactt tagactcttc tatggaagaa cgcatttcta tctgggatgc
     2521 tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa gggccattga cctatatgca
     2581 ctcttatcct cggatacatg ctccttatca tgaacatgcc cacagtcttt atattgatac
     2641 gattctgagt tacggaattg tgggtaccat tttattagtt ttgtcttctg ttgctcctgt
     2701 tcgcttgatg atggatatga gtcaggagtc ggggaaacgt ccgattatcg gcctttatct
     2761 atctttcctt acagtggttg ctgtgcacgg aatttttgac ttggctctct tctggattca
     2821 gtcaggcttt attttcttgc tagttatgtg cagtattcca ttggagcatc gaatgttggt
     2881 atcggacatg acggattaag ttttataagt ttgaaatctt ctaccttggg taggagattt
     2941 ttttatacga tgggcatgtc gtgcatctga ggtgtaagtc ctctgtaggg atccactacc
     3001 ggtgaataca atagcgctcc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct
     3061 ggggatagat cgttttaagt ctgacgcagg aaataagaat tgtcagagaa agcaatagtg
     3121 aaatcgtagg ctatgaatag aaacgtgata gcgaggagca tatagtggat aaggtagcga
     3181 gaaattttaa agtataaaag atagtcttaa tctatatatg taaatcacaa aagtaattga
     3241 attcggaata aggttggtgt gaaaagacat agtaaactga atcttgaata ctagaatgat
     3301 acttctacaa tattgttata atttaatttg aattccaaaa tcacattgtt ccgtccgtat
     3361 ttcattttac tgtatgtctt cctaaactcc aaagtttctg cgtcaaattt cctattttca
     3421 ctgtagtctt tgacacttcg aacgacaatt tgaatcatat attcaaaaaa aaatgaaaaa
     3481 tttgtttgat ttttaatgga taatgtgata taatggttgt caatctataa ttcacataaa
     3541 cagaggagta gaaaatgaaa aaaaatcgtg tatttgctac agcaggtctt gttttattag
     3601 cagcaggtgt acttgcagca tgcagttctt caaaatcatc tgattcatca gcccctaaag
     3661 cttatggcta tgtttataca gcagacccag aaaccttgga ctacctgatt tcaagtaaaa
     3721 atagtacaac agtagtgact tcaaatggga ttgatggttt attcactaac gataattacg
     3781 gtaatcttgc tcctgcagtt gcagaggatt gggaagtctc taaggatggt ttgacctaca
     3841 cttataagat tcgtaaaggg gttaaatggt ttacctctga tggagaagaa tatgcagagg
     3901 tgacggctaa agatttcgtg aacggtttaa aacacgcagc agataaaaaa tcagaagcta
     3961 tgtatttagc tgaaaattcg gttaaaggct tggcagatta tctatcagga acttcaacag
     4021 atttttcaac agttggtgtc aaggcggttg atgattatac gttacaatac actttgaacc
     4081 agcctgaacc gttctggaac tctaagttga cctattctat tttctggcct ctgaatgaag
     4141 aattcgaaac atcaaaagga agcgattttg ctaaaccaac agatccgaca tccttgcttt
     4201 ataatggtcc attcttgttg aaagggttga ctgcaaaatc ttctgtagag tttgtaaaaa
     4261 atgagcaata ttgggataaa gaaaatgtcc acctagatac tatcaatcta gcttactatg
     4321 atggatcaga tcaggagtcg ctagagcgta acttcactag tggagcttat agttatgccc
     4381 gtctttaccc taccagctcc aactattcta aggttgcaga agaatacaag gacaatatct
     4441 attacacaca atcaggctct gggattgctg gtctgggtgt gaatattgat cgccaaagtt
     4501 acaactatac ttctaaaact acagattcag agaaagtagc tactaagaag gcattgctta
     4561 acaaagattt ccgtcaagcc ttgaattttg ctcttgatcg ctcagcttac tcagctcaaa
     4621 tcaatggtaa agatggagca gctttagcag ttcgtaattt atttgtaaaa ccagactttg
     4681 tttcagctgg tgagaagacc tttggtgatt tagtcgctgc tcaacttcct gcttatggtg
     4741 atgagtggaa aggtgtgaat ttagctgatg ggcaggatgg tttattcaat gctgacaagg
     4801 ccaaggcaga gtttgcgaaa gctaagaaag ctttagaagc agacggcgtt cagtttccta
     4861 ttcatctgga cgttccagta gaccaagcat caaaaaacta catatctcgt attcagtcct
     4921 ttaaacaatc tgtagaaaca gttcttggtg ttgaaaatgt cgttgttgat attcaacaaa
     4981 tgacaagtga tgaattcctt aatattactt actatgctgc caatgcttca tctgaggatt
     5041 gggatgtatc gggaggagtt tcatgggggc cagactatca agacccatct acttacctgg
     5101 atattttaaa aacaactagc agtgaaacta caaaaacata tttaggattt gataatccaa
     5161 atagcccttc agtagttcaa gttggtttga aagaatacga taaattagtt gatgaagctg
     5221 ccaaagagac aagcgacttg aatgtccgtt atgaaaaata tgcagcggct caagcatggt
     5281 tgacagatag ttcactcttt attcctgcta tggcttcttc tggtgcagca ccagtgcttt
     5341 cacgaattgt tccatttact ggagcttctg cgcaaacagg ctctaagggg tcagatgttt
     5401 acttcaaata tttgaaatta caagataaag tggtgactaa ggaagagtat gaaaaagctc
     5461 gtgaaaaatg gttgaaagaa aaagctgaat caaatgagaa agctcaaaaa gaattggcaa
     5521 gtcatgtgaa gtaaataatt tctaatagaa ctttctctcc atgaggagaa ggttcttttg
     5581 ggatttttaa aggaaataat atgaaaaaat atatttttat gcgtgttttg cggtcattgg
     5641 tttcgatttt cttagtaacg actttgacct acacgattat ctataccttg gttcctcgaa
     5701 aattgatttt caagcaggat cctaactata ataaaattgc aacaacggct gataaacgtg
     5761 ataactatga aaatactgtg tttgagcgta tgggctacat tgagtattac gatactaaag
     5821 agttgcaaga aaaggcaagt agcatggatt cttctgtaac agtagaagca aatgcgacca
     5881 ataaagctat ttatgaaaag tacatcaatc aattaggtca tggttggact ttgggagaat
     5941 ttactgaaag tggtcaattc tatgctactc gtgaaattcc aatttttgaa cgtgtttttc
     6001 acttctatgc taacttgatt gacattgacc atacaaataa aatccaagac cctgaaaatc
     6061 cagacttgaa acgctacctt cgttttgaaa atgatccagc tatcggatgg tcattggtcg
     6121 gttcaggaac taaacataaa tatctcttgt actttaacag tcagttccca tttgttcatc
     6181 aaaactttgt gaacttgaat ttaggtgact cttacccaac ctatgctaat acaccagttc
     6241 ttcaggttat tactcaaggt caaggacaaa ccaaaactgc ccaagttcag ttcccaacag
     6301 gtaagaaaac gtcttctgta aatatttact caagaaccta caagtcacct agtcaggctg
     6361 actctcgtga agtagctagc tatgggaaag atgatcctta tacagcgact gaaagtaatt
     6421 accaatatcc atctatgatt gtcagctctg ctattactgg tttgattggt ttggttcttg
     6481 cctatgctct tgccgtgcca cttggttcag ccatggctcg tttcaagaac acttggattg
     6541 atagcctctc aacaggggct ttgaccttct tgcttgctct tccaacgatt gccttggttt
     6601 acatcgttcg attgattgga tcatctattg cccttccaga ttcattccct atcttgggag
     6661 ctggagattg gcgttcttac gttttaccag cagtcatcct tggtttgttg ggtgctcctg
     6721 gtacagccat ttggattcgt cgttacatga ttgacttgca atctcaagac tttgttcgtt
     6781 tcgctcgtgc aaaaggtttg tctgaaaaag aaatttcaaa caaacacatc tttaaaaatg
     6841 ccatggttcc gctggtttca ggaattcctg gtgccatcat tggggttatc ggtggtgcaa
     6901 cccttactga aacagtcttc gccttcccag gtatgggtaa aatgttgatt gactctgtaa
     6961 aagcatctaa taactctatg gtcgttggtc ttgtcttcat ctttacatgt atttctatct
     7021 tctcacgtct tttgggagat atttggatga ctattattga cccacgtatt aaattgactg
     7081 agaaaggagg caaataatgt ctacaatcga taaagaaaaa tttcagtttg taaaacgtga
     7141 cgattttgcc tctgaaacta ttgatgcgcc agcatattct tactggaaat cagtgtttaa
     7201 acaatttatg aagaaaaaat caactgtagt catgttggga atcttggtag ccatcatttt
     7261 gataagtttc atctacccaa tgttttctaa gtttgatttc aatgatgtca gcaaggtaaa
     7321 cgactttagt attcgttata tcaagccaaa tgcggagcat tggttcggta ctgacagtaa
     7381 cggtaaatcg ctctttgacg gtgtctggtt cggagctcgt aactccatcc tcatttctgt
     7441 gattgcgaca gtgattaact tggttatcgg tgtttttgtc ggtggtattt ggggtatttc
     7501 aaaatcagtt gaccgtgtca tgatggaagt ttacaacgtc atctcaaaca tcccacctct
     7561 tttgattgtt attgtcttga cttactcaat cggagctgga ttctggaatc tgatttttgc
     7621 catgagcgta acaacatgga ttggtatcgc cttcatgatc cgtgtgcaaa tcttgcgcta
     7681 tcgtgacttg gaatacaact tggcatcacg tactttggga acaccaacct tgaagattgt
     7741 tgccaaaaat atcatgcctc aattggtatc tgttattgtg acaaccatga ctcaaatgct
     7801 tccaagcttt atctcatacg aagccttctt gtctttcttc ggtcttggat taccgattac
     7861 agtgccaagt ttgggtcgtt tgatttcgga ttattcacaa aacgtaacaa ccaatgctta
     7921 cttgttctgg attccattga caacccttgt cttggtatcc ttgtcccttt tcgtagttgg
     7981 tcaaaactta gcggatgcta gtgatccacg tacacataga taggagtaga aatgacaaaa
     8041 gaaaaaaatg taattttgac tgctcgcgat attgtcgtgg aatttgacgt tcgtgacaaa
     8101 gtattgacag ccattcgcgg cgtttccctt gaactcgttg aaggagaagt attagccttg
     8161 gtaggtgagt caggatcagg taaatctgtt ttgacaaaga ccttcacagg tatgctcgaa
     8221 gaaaatggtc gcattgccca aggtagtatt gactaccgtg gtcaggattt gacagcttta
     8281 tcttctcaca aggattggga acaaattcgt ggtgctaaga ttgcgactat cttccaggac
     8341 ccaatgacta gtttggaccc cattaaaaca attggtagtc agattacaga agttattgta
     8401 aaacaccaag gaaaaacagc taaagaagcg aaagaattgg ccattgacta catgaataag
     8461 gttggcattc cagacgcaga tagacgtttt aatgaatacc cattccaata ttctggagga
     8521 atgcgtcaac gtatcgttat tgctattgcc cttgcctgcc gacctgatgt cttgatctgt
     8581 gatgagccaa caactgcctt ggatgtaact attcaagctc agattattga tttgctaaaa
     8641 tctttacaaa acgagtatca tttcacaaca atctttatta cccacgacct tggtgtggtg
     8701 gcaagtattg cggataaggt agcggttatg tatgcaggag aaatcgttga gtatggaacg
     8761 gttgaggaag tcttctatga ccctcgccat ccatatacat ggagtctctt gtctagcttg
     8821 cctcagcttg ctgatgataa aggggatctt tactcaatcc caggaacacc tccgtcactt
     8881 tatactgacc tgaaagggga tgcttttgcc ttgcgttctg actacgcaat gcagattgac
     8941 ttcgaacaaa aagctcctca attctcagta tcagagacac attgggctaa aacttggctt
     9001 cttcatgagg atgctccaaa agtagaaaaa ccagctgtga ttgcaaatct ccatgataag
     9061 attcgtgaaa aaatgggatt tgcccatctg gctgactagg aggaaggaaa tgtctgaaaa
     9121 attagtagaa atcaaagatt tagaaatttc cttcggtgaa ggaagtaaga agtttgtcgc
     9181 ggttaaaaat gctaacttct ttatcaacaa gggagaaact ttctcgcttg taggtgagtc
     9241 cggtagtggg aaaacaacta ttggtcgtgc tatcatcggt ctaaatgata caagtaatgg
     9301 agatatcatt tttgatggtc aaaagattaa tggtaagaaa tcgcgtgaac aagctgcgga
     9361 attgattcgt cgaatccaga tgattttcca agaccctgct gcaagtttga atgaacgtgc
     9421 gactgttgat tatattattt ctgaaggtct ttacaatcac cgtttattta aggatgaaga
     9481 agaacgtaaa gagaaagttc aaaatattat ccgtgaagta ggtcttcttg ctgagcactt
     9541 gactcgttac cctcatgaat tctcaggcgg tcaacgtcaa cgtatcggta ttgcccgtgc
     9601 cttggtcatg caaccagact ttgttattgc agatgagcca atttcagcct tggacgtttc
     9661 tgtacgtgcc caagtcttga acttgctcaa aaaattccaa aaagagctcg gcctgaccta
     9721 tctcttcatc gcccatgact tgtcggttgt tcgctttatt tcagatcgta tcgcagttat
     9781 ttacaagggt gttattgtag aggttgcaga aacagaagaa ttgtttaaca atccaattca
     9841 cccatatact caagccttgc tttcagcggt accaatccca gatccaatct tggaacgtaa
     9901 gaaggtcttg aaggtttacg acccaagtca acacgactat gagactgata agccatctat
     9961 ggtagaaatc cgtccaggtc actatgtttg ggcgaaccaa gccgaattgg cacgttatca
    10021 aaaaggacta aactaataat ggttttataa tttccatgtc aatttttatg ggaattataa
    10081 aacttttttg ttggactgat ttgaaaaact ttgaaagctt cctgaaaga
//
LOCUS       DAVBCR010000071        10086 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCR010000071 DAVBCR010000000
VERSION     DAVBCR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.071"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            588..986
                     /locus_tag="VQ508_001693"
     CDS             588..986
                     /locus_tag="VQ508_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2490660.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            1024..1713
                     /locus_tag="VQ508_001694"
     CDS             1024..1713
                     /locus_tag="VQ508_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490661.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1755..2003
                     /locus_tag="VQ508_001695"
     CDS             1755..2003
                     /locus_tag="VQ508_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490662.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            2033..2644
                     /locus_tag="VQ508_001696"
     CDS             2033..2644
                     /locus_tag="VQ508_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490663.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2805..3599
                     /locus_tag="VQ508_001697"
     CDS             2805..3599
                     /locus_tag="VQ508_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2490664.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3596..4231
                     /gene="trmB"
                     /locus_tag="VQ508_001698"
     CDS             3596..4231
                     /gene="trmB"
                     /locus_tag="VQ508_001698"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2490665.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4357..4836
                     /gene="rimP"
                     /locus_tag="VQ508_001699"
     CDS             4357..4836
                     /gene="rimP"
                     /locus_tag="VQ508_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2490666.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4880..6016
                     /gene="nusA"
                     /locus_tag="VQ508_001700"
     CDS             4880..6016
                     /gene="nusA"
                     /locus_tag="VQ508_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2490667.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6038..6331
                     /locus_tag="VQ508_001701"
     CDS             6038..6331
                     /locus_tag="VQ508_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2490668.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6324..6623
                     /locus_tag="VQ508_001702"
     CDS             6324..6623
                     /locus_tag="VQ508_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2490669.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6640..9432
                     /gene="infB"
                     /locus_tag="VQ508_001703"
     CDS             6640..9432
                     /gene="infB"
                     /locus_tag="VQ508_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2490670.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9683..10033
                     /gene="rbfA"
                     /locus_tag="VQ508_001704"
     CDS             9683..10033
                     /gene="rbfA"
                     /locus_tag="VQ508_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2490671.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3060 a         1847 c         2301 g         2878 t
ORIGIN      
        1 attcaagatt tggatgaaaa attttaaaaa acaatgatat actaaacttg tcaaagttgc
       61 aacgggtgct gctacattat gttaaaaatc gctcttgatt gataagctgg atttgttagg
      121 aaaggagtaa ataattatgg ataagaaaaa gatagtttcg actataatat gtatagtatt
      181 tcttgtagtg tcagtagata atttttttag agacttaacc ccgttactgt tcattttaaa
      241 tattattggc ctgtcttgct tttcagtgct aacatacatt aacataaaag agatactctt
      301 aaatataagt aaatgatgat ttggttagtc ctagaaccaa tcaacttatg tttatcggct
      361 tggcagattt tatgtttgtc atttgtttgt atagaggtat ttcagaaaca gaattctatc
      421 aacaactaat cgcttatata ggtgtattct ctgcttgttt atcacgcttc tgctcatgtg
      481 gggcttgaag tattataaaa agtagctttc tatccttttc tgacaggata tttgagtaga
      541 aggctctatt aaattgttag tgaaataaat gaatggaggt atttaatatg aaatacagat
      601 tattttttgt tattttcttg agtagtgtgt tggatattct tttagggaca tttttacaaa
      661 tctctatcgt atctattggg tggcttgttc tttacagtgg tttgtttgaa gtaggagttt
      721 tccttcttgc taataaaggg gtggcggtaa aaatcaagga agtagatatt cgaaatcgct
      781 ttaaatttat ttttggaaaa accttatggt ttcaaattct tttgctcatc tttttgataa
      841 tcaaacttta tcttggtttg gatgcgaggt tgattttatt ctatggacat attttcattg
      901 tctttaatgc cttaatgtat ctattatcta gtagtcaggt tagccttaaa aaaaaacaaa
      961 ctgtcttctt aatcttacag cagtagagtc tgacaaggtg aggtcatagt agggaggatt
     1021 tctatgaaaa agtatcaact tctattcaaa ataagtgcag tcttctctta cttatttttc
     1081 gtatttggtc tttctcagct gacgcttatc gtccaaaatt attggcaatt ttcttcccag
     1141 attggtaatt tcgtctggat tcaaaatatc ttgagtttac tttttattgg agtcatgatt
     1201 gtggttcttg ttaagacagg ccatggttat ctcttccgca ttccaagaaa aaaatggctt
     1261 tggtattcga ttttgacagt attagtggta gtgttccaga tctcttttaa cgttcagaca
     1321 gctaaacatg ttcagtcaac tgcggaaggt tgggctgtat tgattggtta tagtgggact
     1381 aactttgcag agctaggtat ttatatagcc ctgttctttc tggttccact gatggaagaa
     1441 ttgatttata gaggattact gcaacatgct ttctttaagc attcgcgatt tggtcttgat
     1501 ttgcttcttc cttctatttt atttgctctc cctcattttt caagcctgcc tagtctgtta
     1561 gatatcttcg tctttgcaac agttggaatc atctttgctg gtttgacccg ctataccaag
     1621 agcatttatc catcctatgc ggtgcatgtg atcaataata ttgtagcgac cttcccgttt
     1681 ttgctcactt ttctacatag ggtcttgggg taaaaaaaca aaaggcttgc ttttcagcca
     1741 tagaggaggt catcatgtat aaacacttat ttttcctaga ttccaaaact ttagatcggt
     1801 tgacacccta tattctagtc ttggcttctg acaccattgc ttttaatgtt tttgtgctaa
     1861 cctttgtatc tgcggtggtt tttaatttcc taaattccat gctagcttta atggctatat
     1921 tcataggggc tggctatgtg gtcggatttt ggttgctaaa atggtttgtt ttggaaaggt
     1981 tagagctaaa ggatgacgcg taggaagtct gtttggtgag gaggatagtt ttatggagtt
     2041 ttttgataaa tttcatgcct tgtgttttgg atttttagta ctaataattg tcattacagt
     2101 tccttatacg attaaccatg ggggtttttt tcaaaatgaa tctgcattga ttcttgtaag
     2161 tcttcttgta acctcgctga gtgttgctta tgctagaaag tttgaaatga tttcttttgg
     2221 gatgttaagc aagaaacaac ttttgctttt cattgcaatc tttcttctaa gtgtacttga
     2281 gacgctggtt tatattcatt tcttcgctgt ttcttctggc tcaggggtcc aacacttggc
     2341 ggaagtcagc agaggaattt ccctgtcttt gattttgact acctcagttt ttggccccat
     2401 ccaggaggaa ctcattttca gaggacttct tcaaggtgcg gtttttgaca attcttggtt
     2461 agggcttgtg ctaacttcct ctctcttttc tttcatgcat ggaccttcta atgtcccttc
     2521 gtttattttt tatctacttg ggggtttgtt gctgggcttt gcttataaaa agagtcaaaa
     2581 cctatgggtt tctactctag tacacatgtt ttacaacagt tggccactcc tatattattt
     2641 ataaaaatta tgaaaaggta agcagaagac ggtgcttacc tttttcaagc tataatgata
     2701 cccaagccaa tccagaggct tttctttgag aatcgggtta gagtggttga cgaataggcc
     2761 aaaaactagt agaatagtaa ggaaacttta tacggaggaa agaaatggat ttgggtgata
     2821 atgagctaac actgactccc atacctggga aaagtggcaa ggcttatatg ggtagctatc
     2881 ctgacgggaa gcgcatcttt gtaaaaatga acacctctcc aatcctacct ggtctagcta
     2941 gagaacaaat tgctccacaa ttattatgga gtcgtcgttt ggcagatggg cgtgatatgt
     3001 gtgctcaaga atggttgaca ggcaagatat tgacccccta tgatatgaat cgtaagcaaa
     3061 tcgtcaatat tttaacccgt cttcatcgct cacgtccgtt gatgacacaa ttgagtcgtt
     3121 tgggctatgc catggaaaca cctgtagatt tactacagtc ttggcaggaa acggctccag
     3181 atgctttgcg taaaaatcat tttatcagtg aagtgatggc tgatttacgt cagactattc
     3241 caggatttag agaggaccat gcgaccattg tccatggaga tgtacgacat agtaattgga
     3301 ttgagacaga tagtggcttg atttatttag tagattggga ttcggttcgc ttgaccgatc
     3361 gcatgtttga tgtggcccat atgctctgcc attatatttc agaacatcag tggaaggaat
     3421 ggttgaccta ctacggttac aagtacaatc aaacggtatt aagtaaattg tattggtatg
     3481 gtcaattgtc ttatttgagt cagatttcca agtattatat gaaccaagat ttagaaaatg
     3541 tcaatcggga gattcatggt ttgcgtcatt tccgagacaa gtatggaaag agaagatgag
     3601 agttagaaat cgtaaagggg caacagaatt actagaggca aatccccagt atgtggtcct
     3661 caatcccttg gaagccaagg caaaatggcg ggacttgttt ggcaatgata atcccattca
     3721 tgtggaagtt ggaagtggaa agggtgcctt tgtttcaggt atggccaagc aaaaccctga
     3781 catcaactat atcgggattg atattcaaaa gtctgttttg agctacgctt tggacaaggt
     3841 gcttgaagtt ggagtgccta acatcaagct cttgtgggta gatggttctg acttaactga
     3901 ctattttgaa gacggtgaga ttgatcgctt gtatctgaac ttttcagatc catggccgaa
     3961 aaaacgccat gaaaagcgtc gtttgaccta caagaccttc ttggatacct tcaaacgtat
     4021 cttgcctgaa aatggagaaa ttcatttcaa gacggataac cgtggcttgt ttgagtacag
     4081 tttagtgagc ttttctcaat atggcatgaa actcaatggt gtctggttag atttgcatgc
     4141 cagtgatttt gaaggcaatg tcatgacaga atacgagcaa aaattctcaa acaaggggca
     4201 agttatctac cgagttgagg cagaatttta agagatagcc taaaattagg ctgtacaagt
     4261 gcttttgctt tacataagtt ggcaaacgtg ctatactgat agtaagaata tgaaaagtga
     4321 ggcggggaaa tatcttcgcc tcttgcttat gaggaggtgg acgcaatcgc aacaatcgta
     4381 gaattagtca gagaagttgt agaacctgtc atagaagctc cttttgaact cgtggatatc
     4441 gagtatggaa agattggcag tgacatgatt ctcagtattt ttgtagataa acccgaagga
     4501 attaccttga acgacacggc agacttgaca gaaattatca gtcctgtcct agacaccatc
     4561 aagccagatc ccttcccaga acaatatttc ctagaaatta ccagtccagg tttggaacgc
     4621 cctttgaaaa ccaaggatgc cgtcgctgga gcggttggaa aatacatcca tgtcgggctc
     4681 taccaagcca tcgataagca aaaggtcttt gaaggaacct tgttggcctt cgaagaggac
     4741 gagttgacta tggaatatat ggacaagacg cgtaagaaaa ccgtccaaat tccatacagt
     4801 ttagtatcaa aagcacgttt agcagttaaa ttatagaaaa agaaaggata gcttttgagg
     4861 attcaaaagt gaagaaaaca tgagtaaaga aatgctagag gccttccgca ttttggaaga
     4921 agacaaggga atcaaaaaag aagatatcat cgacgcagta gtagagtcgc ttcgttccgc
     4981 ttatcgcaga cgctatggtc agtcagacag cgtagctatt gacttcaacg aaaaaacagg
     5041 tgactttaca gtttatactg tccgtgaagt tgttgatgaa gtatttgata gccgtttgga
     5101 aatcagcttg aaagatgctc ttgccattaa ttcagcttat gaacttggag acaaaatcaa
     5161 gtttgaagaa gcaccagctg agtttggtcg tgtagcagcc cagtctgcca aacaaaccat
     5221 catggaaaaa atgcgcaagc aaacacgtgc catcacttac aatacttaca aagaacatga
     5281 gcaagaaatc atgtctggta cagtagaacg ctttgacaac cgctttatct atgtcaacct
     5341 tggtagcatc gaagcccaat tgtcaaaaca agaccaaatt cctggagaag tttttgcttc
     5401 tcatgatcgt atcgaagttt atgtttacaa ggttgaagac aaccctcgtg gtgtgaacgt
     5461 ctttgttagc cgtagtcatc cagaaatgat caaacgttta atggagcaag aaattccaga
     5521 agtttatgat ggaactgttg aaatcatgag cgtggctcgt gaagcaggtg accgtacgaa
     5581 ggttgctgtt cgtagccaca atccaaacgt ggatgctatc ggtacaatcg ttggacgtgg
     5641 tggtgctaat atcaagaaga ttactagcaa attccaccca gctcgttacg atgctaaaaa
     5701 tgaccgcatg gtaccaatcg aagaaaatat cgatgttatc gagtgggtag cagatccagc
     5761 tgaatttatc tacaatgcca tcgctcctgc tgaggttgac caagttatct ttgatgaaaa
     5821 cgacagcaaa cgtgccttgg tggttgttcc agataacaag ctttctcttg ccattggtcg
     5881 tcgtggacaa aacgtgcgct tggcggctca cttgactggt taccgtatcg atatcaagtc
     5941 tgctagcgaa tttgaagcca tggaagacgc tgcttcagta gagttggaag tagaaaacga
     6001 tactgtagaa gaataaaagc tgctagagga gggaaagatg aaaacaagaa aaatcccttt
     6061 gcgcaagtct gttgtgtcta acgaagtcat tgataagcgt gatttgctcc gcattgtcaa
     6121 gaacaaggaa ggacaagtct ttattgatcc tacgggcaag gccaatggcc gcggcgctta
     6181 tatcaaacta gacaatgcag aagccctaga ggcgaaaaag aagaaggtct ttaaccgcag
     6241 ctttagcatg gaagtggaag aaagctttta tgacgagttg atcgcttatg tggatcacaa
     6301 agtgaaaaga agagagttgg gacttgaata agcaaaagat aagtaatctc ttggggcttg
     6361 ctcagcgagc agggcgcatc atatcgggtg aagaattggt ggtcaaggcc attcaagacg
     6421 gcaaggccaa gttggtcttt ctagctcatg atgctggacc caatctgacc aagaagattc
     6481 aagataaaag tcattattat caagtagaaa ttgtaaccgt gttttcaaca ctggaattaa
     6541 gcatagcagt cgggaaatcg agaaaggttt tggctgtaac agatgctgga tttacaaaga
     6601 aaatgaggtc tcttatggaa tagaagagga ggacatgatt tgtctaagaa aagattgtac
     6661 gaaatcgcaa aagaacttgg aaaagaaagt aaagaagttg tagcgcgtgc aaaagagttg
     6721 ggcttggatg tgaaaagcca ctcatcaagt gtggaagaag ctgtcgctgc aaaaattgct
     6781 gccagcttta agcctgcagc tgctccgaaa gtagaagcaa aacctgcagc accaaaagta
     6841 agtgcagaaa agaaagccga aaaatctgag ccagctaaac cagctgtagc taaggaagag
     6901 gcaaaaccgg ctgagccagt tgctccgaaa acagaaaaag tagcagtgaa accgcaaagt
     6961 cgtaatttca aggctgagcg tgaagcccgt gctaaagagc aggcagaacg acgcaagcaa
     7021 aataagggca ataaccgtga ccaacaacaa aacggaaacc gtcagaaaaa cgacggccgt
     7081 aatggtggaa aacaaggtca aagcaaccgc gacaatcgtc gctttaatga ccaagctaag
     7141 aagcagcaag gtcagcaaaa acgtagaaat gagcgccgtc agcaagagga taaacgttca
     7201 aatcaagcgg ctccacgtat tgactttaaa gcccgtgcag cagccctaaa agcagagcaa
     7261 aatgcagagt acgctcgttc aagtgaggaa cgcttcaagc agtatcaggc tgctaaagaa
     7321 gccttggctc aagctaacaa acgcaaggaa ccagaggaaa tctttgaaga agcggctaag
     7381 ttagctgaac aagcacagca agttcaagca gtggttgaag tcgtccctga gaaaaaagaa
     7441 cctgcagtgg atacacgtcg taaaaaacaa gctcgaccag acaaaaatcg tgacgattat
     7501 gatcatgaag aagatggtcc tagaaaacaa caaaagaatc gaagtagtca aaatcaagtg
     7561 agaaatcaaa agaatagtaa ctggaataac aacaaaaaga acaaaaaagg caataacaag
     7621 aacaaccgta atcagactcc aaaacctgtt acggagcgta aattccatga attgccaaca
     7681 gaatttgaat atacagatgg tatgaccgtt gcggaaatcg caaaacgtat caaacgtgaa
     7741 ccagctgaaa tcgttaagaa acttttcatg atgggtgtca tggccacaca aaaccaatcc
     7801 ttggatgggg aaacaattga actcctcatg gtggattacg gtatcgaagc caaacaaaag
     7861 gttgaagtgg ataatgctga catcgaacgt ttctttgtcg aagatggtta tctcaatgaa
     7921 gatgaattgg ttgagcgtcc accagttgtt actatcatgg gacacgttga ccacggtaaa
     7981 acaacccttt tggatactct tcgtaactca cgtgttgcga caggtgaagc aggtggtatt
     8041 actcagcata tcggtgccta ccaaatcgtg gaaaatggta agaagattac cttccttgat
     8101 acaccaggac acgcggcctt tacatcaatg cgtgcgcgtg gtgcttctgt taccgatatt
     8161 acgatcttgg tcgtagcggc agatgacggg gttatgcctc agactattga agccatcaac
     8221 cactcaaaag cagctaacgt tccaatcatc gtagccatta acaagattga taaaccaggt
     8281 gctaacccag aacgcgttat cggtgaattg gcagagcatg gtgtgatgtc aaccgcttgg
     8341 ggtggagatt ctgaatttgt tgaaatctca gctaaattca accaaaatat cgaagaattg
     8401 ttggaaacag tccttcttgt agctgaaatc caagaactca aggcagaccc aacagtgcgt
     8461 gcaatcggta cggttatcga agcgcgcttg gataaaggaa aaggtgcggt cgcaaccctt
     8521 cttgtacaac aaggtacctt gaatgttcaa gacccaatcg ttgtcggaaa taccttcggt
     8581 cgtgtccgtg ctatgaccaa cgaccttggt cgtcgtgtta aagttgctgg accatcaaca
     8641 ccagtctcta tcacaggttt gaacgaagca ccgatggcgg gtgaccactt tgccgtttac
     8701 gaggatgaaa aatctgcgcg tgcagcaggt gaagagcgtg ccaaacgtgc cctcatgaaa
     8761 caacgtcaag ctacccaacg tgttagcctt gaaaacctct ttgataccct taaagctggg
     8821 gaactcaaat ctgttaatgt tatcatcaag gctgatgtac aaggttctgt tgaagccctt
     8881 tctgcctcac ttcaaaagat tgacgtggaa ggtgtcaaag tgactatcgt ccactcagcg
     8941 gtcggtgcta tcaacgaatc agacgtgacc cttgccgaag cttcaaatgc ctttatcgtt
     9001 ggtttcaacg tacgccctac accacaagct cgtcaacaag cagaagctga cgatgtggaa
     9061 atccgtcttc acagcattat ctacaaggtt atcgaagaga tggaagaagc tatgaaaggg
     9121 atgcttgatc cagaatttga agaaaaagtt attggtgaag cggttatccg tgaaaccttc
     9181 aaggtgtcta aagtgggaac tatcggtgga tttatggtta tcaacggtaa ggttgcccgt
     9241 gactctaaag tccgtgttat ccgtgatggt gtcgttatct atgatggtga actcgcaagc
     9301 ttgaaacact ataaagacga cgttaaggaa gttacaaacg gccgtgaagg tggattgatg
     9361 atcgacggct acaatgatat taagatggat gatgtgattg aggcgtatgt catggaagaa
     9421 atcaagagat aagatttttt gctcctttct taggtggtga gggacgcaag caaaccgatg
     9481 gtttcattgc ttatttttga gcctagggtc tcaaaaatcc cctgtgatgg gactgataaa
     9541 tcagttccat cactttcacc acggcgaaag aagcagatga cttcaaattg aacttcgttt
     9601 caatttaaac tgaaaatcaa gaagtttaaa atagctaggt ctgctggcct agcttttggt
     9661 tcaaagtaga gaaaggaata tcatggcaaa tcatttccgt acagatcgtg tgggcatgga
     9721 aatcaagcgt gaagtcaatg agattttgca aaagaaagtc cgtgatccac gtgtccaagg
     9781 tgtgaccatc acagatgttc agatgctggg tgacttgtct gttgccaagg tttattacac
     9841 cattttgagt aaccttgctt cggataacca aaaagcccaa atcgggcttg aaaaagcaac
     9901 tggtaccatc aaacgtgaac ttggtcgcaa tttgaaattg tacaaaatcc cagatttgac
     9961 cttcgtcaaa gacgagtcca tcgagtatgg aaacaagatt gacgagatgc tacgcaatct
    10021 ggataagaac taaagaagag gggttgcccc tctttttcgg ctctttgtca actgtagtgg
    10081 gttgaa
//
LOCUS       DAVBCR010000072         9760 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCR010000072 DAVBCR010000000
VERSION     DAVBCR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.072"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            271..366
                     /locus_tag="VQ508_001705"
                     /pseudo
     CDS             271..366
                     /locus_tag="VQ508_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(619..1239)
                     /locus_tag="VQ508_001706"
     CDS             complement(619..1239)
                     /locus_tag="VQ508_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2490672.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1239..1721)
                     /locus_tag="VQ508_001707"
     CDS             complement(1239..1721)
                     /locus_tag="VQ508_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2490673.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1721..2563)
                     /locus_tag="VQ508_001708"
     CDS             complement(1721..2563)
                     /locus_tag="VQ508_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2490674.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2658..3458)
                     /locus_tag="VQ508_001709"
     CDS             complement(2658..3458)
                     /locus_tag="VQ508_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2490675.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3602..4981
                     /gene="glmU"
                     /locus_tag="VQ508_001710"
     CDS             3602..4981
                     /gene="glmU"
                     /locus_tag="VQ508_001710"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2490676.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4991..5536
                     /locus_tag="VQ508_001711"
     CDS             4991..5536
                     /locus_tag="VQ508_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2490677.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5548..5862
                     /locus_tag="VQ508_001712"
     CDS             5548..5862
                     /locus_tag="VQ508_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2490678.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5879..6571
                     /locus_tag="VQ508_001713"
     CDS             5879..6571
                     /locus_tag="VQ508_001713"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2490679.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6648..7139
                     /gene="rocS"
                     /locus_tag="VQ508_001714"
     CDS             6648..7139
                     /gene="rocS"
                     /locus_tag="VQ508_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2490680.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7237..7827
                     /locus_tag="VQ508_001715"
     CDS             7237..7827
                     /locus_tag="VQ508_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2490681.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7836..9599
                     /locus_tag="VQ508_001716"
     CDS             7836..9599
                     /locus_tag="VQ508_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2490682.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3004 a         1699 c         2084 g         2973 t
ORIGIN      
        1 caccccaaaa gttagatttt ttctgtctaa cttttggggt gtagttcatg tacacctgat
       61 atgatgcgtt ttataatttt aaagactttt tgaccagcct cattttttta acttgatact
      121 cagtgaaaag caaagattaa actaggaagc tagctgtagg ctgctcaaag aacagctttg
      181 aggttgcaga taaaacttgt gaggtcacca acatatataa tgtgaagctg acgtggcttg
      241 aatagatttt agaagggtat gagtctggaa gttttaatgg ataatgcaag attccataga
      301 atgggtaagc tagagttctt atgtgaagag tttgggcata aacttttacc ttttcctccc
      361 tactcatctt agtatagaaa agtgaatcta aaatagtaca taactgcttc taaaacattc
      421 ttataaattg atttaaattc tcaaatcata ttattcagtt cttatttcat tttgctctac
      481 aatcctgttg agaagacacg tgttcatatc aaaaaggtat tggcaagttg caataccttt
      541 ttacaaggtt cttttgtctt atttttgttt caactgacta tatctcctat ggttctagtt
      601 cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa
      661 tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt
      721 acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc
      781 gtgtccgaca attagaatac gctcagctgg actatctttt aatgatttga taaattggat
      841 ggtccgttga gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc
      901 aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaagg ttgcaatttt
      961 caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca
     1021 gggggtatag agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact
     1081 tgaataaatc tgatcaaaag gaatttcctt gagatactgg ccaagtcgtt ttagggtttc
     1141 aatggattca ggaagaagag gagaatcacc gctagcacct tgaaaacgac cttcttggtt
     1201 ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata
     1261 tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt
     1321 ccgacttcgc ctgcagagac aatcattcga tccaaatcta gagcaatttt atcgataaaa
     1381 atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca
     1441 gttgtttttt ctaagtcctt ttgtggaatc atgctcactt ttttattgcc agaaatttta
     1501 gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc
     1561 ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc
     1621 tctccttcta gggcattgat ttgaatcccc tgatgaggga tagctgcttt agataggatt
     1681 tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca
     1741 attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat
     1801 aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta
     1861 agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt
     1921 agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa
     1981 ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag
     2041 catagagtcc caaaatcaaa agtaagttcg atgggctgag gccaggaacc aagacgccaa
     2101 gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga
     2161 caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagcta
     2221 aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac
     2281 cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt
     2341 caatcgggta ggaaaataag ccgattccca gaagcatacc gatggcaact ggaataaagt
     2401 acaaaacatt ttctttaagg tctttaaagg gatgggccag aaagccaatc attcgttcat
     2461 agattcctaa gattgctgct agaacccctc cggaaattcc cggtaggata aatccaagag
     2521 caattacaat ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat
     2581 ttctatttca aaaattcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa
     2641 atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt
     2701 tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta
     2761 gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctctc caaaaatcct
     2821 gagtctgagc cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt
     2881 tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacctag tgatgctagt
     2941 tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca
     3001 agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt
     3061 tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg gacattccgc
     3121 ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca
     3181 tcattttctt ttgttgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt
     3241 tgtgagaggg catcgtagtt gatttcctca ggacgctgcc agccagagag gtcaataatc
     3301 ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta
     3361 aacgattggc tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatcccaaaa
     3421 aaactaaata aaataagtgg atgaatttgt tttctcatat cttataagtc taccctaaaa
     3481 atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag
     3541 agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat
     3601 tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt
     3661 gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt
     3721 gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga
     3781 ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca
     3841 tgcagttatg atgacagaac ctatcttaga aggtttgtca ggacacacct tggtcattgc
     3901 aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa
     3961 tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg
     4021 aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga
     4081 ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt
     4141 gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga
     4201 cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt
     4261 tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg
     4321 tcgtcgcatc aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac
     4381 ttatatcgat attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttacctt
     4441 gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga
     4501 cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc
     4561 agacggtgtg acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca
     4621 agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc
     4681 tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg
     4741 aactattaca gtcaactatg acggcaaaaa caaatacaag acagtcattg gagacaatgt
     4801 ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt
     4861 tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg
     4921 tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta
     4981 ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc
     5041 aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccgacg
     5101 ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact
     5161 tatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg
     5221 aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga
     5281 agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt
     5341 ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg
     5401 tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga
     5461 attaatccaa tcaggtcata tctgtgatgc caagacaatt atggctgttc agtattggga
     5521 gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt
     5581 gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggaa
     5641 actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa ggatcatggt
     5701 tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgtatt
     5761 gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt tgcggtcatc
     5821 tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat
     5881 gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga
     5941 taatacccag gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca
     6001 tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat
     6061 tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc
     6121 agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga
     6181 tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc
     6241 agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca
     6301 tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat
     6361 taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc
     6421 agcgcatgcc ctcaatctcc cagtcttagt catcagagct atgagtgaca atgccaacca
     6481 tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt
     6541 cttgttgacc tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat
     6601 gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa
     6661 tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg
     6721 tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca
     6781 gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgagcctg
     6841 tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag
     6901 aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg
     6961 agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac
     7021 aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag
     7081 caaaagaaga agtccaatcc actaagaaag gcttttttgc tcgtttattt ggaggataat
     7141 caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag
     7201 ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt
     7261 gcttttgact tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca
     7321 gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc
     7381 cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct
     7441 cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata
     7501 ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc
     7561 caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat
     7621 ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct
     7681 cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg
     7741 gatcaagcta gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtgtc
     7801 ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat
     7861 tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt
     7921 ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag
     7981 atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc
     8041 cattgattag tctagctttt ttagccttga ttgtaattaa ccttctggtt gcttattttc
     8101 aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa
     8161 ttgagtatat ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag ctaacttgcg
     8221 ctaaagcctt gtttatcttt ttctatatag ctatgctttt tccttttata cggaaaatgc
     8281 tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag
     8341 ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct
     8401 ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag
     8461 cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat
     8521 tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg
     8581 ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt
     8641 ttgtcgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt
     8701 catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc
     8761 gatggggtgt catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg
     8821 aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg
     8881 ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg
     8941 agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga
     9001 aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga
     9061 ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc
     9121 gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc
     9181 cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc
     9241 aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc
     9301 cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata
     9361 ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga
     9421 aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag
     9481 tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag
     9541 aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt
     9601 aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc
     9661 ttttttgtgg ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt tcaatttact
     9721 ttatagtgct ttgaagttgg aatgctacac tataacttct
//
LOCUS       DAVBCR010000073         9651 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCR010000073 DAVBCR010000000
VERSION     DAVBCR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.073"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(302..1282)
                     /gene="dusB"
                     /locus_tag="VQ508_001717"
     CDS             complement(302..1282)
                     /gene="dusB"
                     /locus_tag="VQ508_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2490683.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1269..2141)
                     /gene="hslO"
                     /locus_tag="VQ508_001718"
     CDS             complement(1269..2141)
                     /gene="hslO"
                     /locus_tag="VQ508_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2490684.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2306..3733
                     /locus_tag="VQ508_001719"
     CDS             2306..3733
                     /locus_tag="VQ508_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2490685.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3892..5400
                     /gene="glpK"
                     /locus_tag="VQ508_001720"
     CDS             3892..5400
                     /gene="glpK"
                     /locus_tag="VQ508_001720"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2490686.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5442..7268
                     /gene="glpO"
                     /locus_tag="VQ508_001721"
     CDS             5442..7268
                     /gene="glpO"
                     /locus_tag="VQ508_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2490687.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7338..8042
                     /locus_tag="VQ508_001722"
     CDS             7338..8042
                     /locus_tag="VQ508_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2490688.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8339..8542
                     /locus_tag="VQ508_001723"
     CDS             8339..8542
                     /locus_tag="VQ508_001723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490689.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8629..9216)
                     /locus_tag="VQ508_001724"
     CDS             complement(8629..9216)
                     /locus_tag="VQ508_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490690.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2795 a         1922 c         2181 g         2753 t
ORIGIN      
        1 tcaggtgccc ttgcagtcaa cacaactgta gatagctata gagtcaatgc caatggtgaa
       61 tgggtaaact aaacctaata taactagtta atactgactt cctgtaagaa ctctttaaag
      121 tattccctac aaataccata tcctttcagt agataatata cccttgtagg aagtttagat
      181 aaaaaaataa ctctgtaatc tctagccgga tttatagcgc tagagactac ggagtttttt
      241 tgatgaggaa agaatggcgg cattcaagag gctctttaag agagttacgg gttttaaact
      301 attaagcctt ctccaattgc aagagggctt caatctctgc cagggtgcta gcttgcgaaa
      361 tggctccacg gagtttggca gcgccagatg ttccacggag atagtgagga gcgaggccgc
      421 ggaattcacg aactgcgacg ttttctcctt tgaggttaat caatcgtttc aagtgttcgt
      481 aggcgatctt catcttgtct tcaaaggtca aatcaggtag gatttctcct gtttcaaagt
      541 aatggttgat ttggttgaag aggtaaggat ttcccatggc agctcggcca atcatgactg
      601 cgtcagcacc aacttcttcg atgcgttgct tggcttcttg gacagtacgg atatcaccgt
      661 tggcgatgaa tggaatcttg gttagagctt gggcaacctt gtaaagggtc tcaaggtctg
      721 cgtggccagt atacatttgt tcacgggtac ggccatgcat ggcgagggca gaaacacctg
      781 cagcctcagc agcgagggca ttttctactg caagagatgg gtccgcccat ccggtacgca
      841 ttttgacagt aagtgggata tcaaggacag actggacctt gttgatgata gagtagatct
      901 tgtcaggatc cttgagccac atagctccag cttcgttctt cacgattttg ttgacagggc
      961 agcccatgtt gatatcgacg atatcggtct tggtgttttc ttggatgaat tctgctgcgc
     1021 gtgctaggct gtcttcatcg ctaccaaaaa gttggataga gacagggttt tcgccctcat
     1081 cgatatgaag catgtgcagg gttttttcgt tgttgtattg gattcccttg tcagagacca
     1141 tttccattac aacgagtcca gctccgagct cttttgcgat ggtacgaaag gctgagttgg
     1201 tcacgccagc cataggcgct aaaacggtac gattgggaat ctcaatattg ccaatcataa
     1261 aaggtgtatt aagatttgtc acgaatgagt tcctccaggt ccttttcatc aaagttgtaa
     1321 gtagtttggc agaattgaca agtgatttct gccccgtggt cttcctcttt catttcctgt
     1381 aagtctgagc ttggaaggct ggcaagagcg ttcataaagc gttcatggct acagtcacat
     1441 tggaaacgga tttcttcttc agaaagacgc ttgtaggctt cgtccccgta gatagccttg
     1501 aggagggctt cgatatggtc gtcgctttcg agaagagtag agatagctgg catttcttgg
     1561 atgcgttttt caaagcgagc aatctcttct ttcttggctc ctggcaagac ttgaactagg
     1621 aaaccacctg caaccttgac cttgtcttcc tcgtccaaaa ggacattgag gccgaccgct
     1681 gaaggcgttt gttggctttc agtaaggtaa aaggcaaggt cttcaccgat ttctccagag
     1741 atgaggggag ttatagagtt gtaaggattt ccagcaccgt agtctgtgat aacgaggaat
     1801 tgaccatttc caacaaaagg tccgactagg acttcaccag tcgcagtctt tttgatgtca
     1861 acaccaggat tttgaacata gcctttgacg ttccccttgg tatcagcgac ggtgataata
     1921 gcacctagag agctagagcc caacacctta actgtaagtt tggtatttcc tttttcattg
     1981 gctgcgagaa tctggctagc gataagagtt cgaccaagcg ctacagttga gctagcttgg
     2041 gtttgatgtc tttcttgagc agtgcggacg gtttccgtgc tatcaaggac aaaagcacga
     2101 aaggctccgc tttctgatat agttttaata attttatcca tagctactat tttagcataa
     2161 aaatgcccaa agggggagcc gtgtgtttac tgattttcag gataatggac caggaaatca
     2221 gcatgaaaat aaaaagagaa acagattatt tcagcatttg taagatttat gctatgctta
     2281 aggtagaaaa tgaaaggggt aacaaatgta tttaggagat ttgatggaga aagccgagtg
     2341 tggtcaattt tcaatccttt cctttctatt acaagagtct cagacgaccg tcaaggctgt
     2401 aatggaagaa acaggatttt caaaagcaac cctaaccaaa tatgtcaccc tgctcaatga
     2461 caaggctttg gatagtggct tagaactgac tattcactca gaagatgaaa atctgcgtct
     2521 gtctatcggt gcagctacca aggggagaga tattcggagc ttgtttttgg atagtgctgt
     2581 taaataccag attttggttt atcttctcta ccaccaacag tttttagccc atcagctggc
     2641 tcaagaattg gtgattagcg aggctacgct tggtcgtcac ttggctggtt taaatcagat
     2701 tttgtcagaa tttgatttat ccatccaaaa tggacgctgg agaggtccag agcatcagat
     2761 tcgctatttc tatttctgtc ttttccgaaa ggtctggtcg agtcaggaat gggaaggtca
     2821 catgcagaaa ccagagagaa aacaggagat tgccaattta gaggaaatct gcggtgcaag
     2881 tttgtctgcg gggcagaaat tggacttggt tctctgggct cacatcagtc aacaacgtct
     2941 tcgggtcaat gcttgtcagt ttcaagtcat agaagagaaa atgcgagggt attttgacaa
     3001 tatcttttat cttcgtttgc tgagaaaggt tccgtccttt tttgctgggc aacatattcc
     3061 actaggagtt gaggatggtg agatgatgat attcttctct tttctcctat ctcatcgcat
     3121 tcttcctctt catactatgg agtatattct tggttttgga gggcagttgg cagatttact
     3181 gacgcaattg attcaagaaa tgaagaagga ggaactattg ggggattata cagaggacca
     3241 tgtcacctat gaactcagtc agctttgtgc tcaagtctat ctctataagg gctatatttt
     3301 acaggatcgc tacaagtacc agttagagaa tcgtcatcca tatttactga tggaacatga
     3361 ttttaaagag acagcagagg agatttttca tgctctacct gcttttcaac aggggacaga
     3421 tttagataag aagattctct gggaatggct ccagttaatc gaatatatgg ctgaaaacgg
     3481 tggccagcat atgcggattg gtctggattt gacatctggt tttcttgtct tttcaaggat
     3541 ggcagccatt ttgaaacggt atttggaata caatcgtttt attaccattg aagcttatga
     3601 ccctagtcgg cattatgatt tgctggttac caataacccg attcataaga aggaacagac
     3661 accagtctat tatttaaaaa acgacttgga tatggaagat ttggcaggga ttcgtcagtt
     3721 attattcact taaaaggctt ggttaatcta ggtctttttt gtgaaattca cacaatctcc
     3781 tcacattttt ttaaaaatta aaaaaagttg ataaacaaga aagcgcttta ttttgtatac
     3841 tagtaagtgt aaagaggaaa cacctcaaga tctttatcag gaggacagta catgtcacaa
     3901 gaaaaataca tcatggccat tgaccaggga actacaagtt ctcgtgccat cattttcaac
     3961 aaaaaagggg aaaaggttag ctcgagtcaa aaagagttta cccagatttt ccctcaggca
     4021 ggttgggttg agcacaatgc caatgaaatt tggaactctg ttcagtcagt tattgcgggt
     4081 gctttcatcg aaagtggtgt caagccaaat caaatcgagg caatcgggat taccaaccaa
     4141 cgtgaaacaa cggttgtctg ggataagaaa acaggacttc ctatctacaa tgctatcgtt
     4201 tggcagtcac gccagacagc acctttggct gagcaactaa aaagccaagg ttatgtggaa
     4261 aaattccatg aaaagactgg tttgattatt gatgcttact tctctgctac caaggttcgt
     4321 tggattttgg atcatgtaga aggtgctcaa gagcgagcag aaaaagggga attgctcttt
     4381 ggtactatcg atacttggtt ggtttggaaa ttgactgacg gtgcggctca cgtgactgac
     4441 tactcaaatg cagctcgtac catgctttat aacattaaag aactcaaatg ggatgatgag
     4501 attttggaaa tccttaacat tccgaaggct atacttccag aagttcgttc taactccgaa
     4561 atctacggca agacagctcc attccatttc tacggtggag aggtgccaat ctcaggtatg
     4621 gctggggacc aacaagcagc cctctttgga cagttggctt ttgagccagg tatggttaag
     4681 aatacttatg gaacaggctc tttcatcatc atgaatactg gggaagagat gcagttgtct
     4741 gaaaacaacc tcttgacaac cattggttac ggaatcaacg gtaaggttta ttatgccttg
     4801 gaaggttcta tcttcatcgc aggaagtgct attcagtggc ttcgtgacgg tcttcgcatg
     4861 gttgaaaatt caccagaatc tgaaaaatac gctcgtgatt ctcacaacaa cgatgaagtt
     4921 tatgtcgttc cagcctttac aggtctaggc gctccatact ggaaccaaaa tgctcgtggt
     4981 tccgtctttg gtttgactcg tggaacaagc aaagaagact ttatcaaggc gactttgcaa
     5041 tctattgctt atcaagtgcg tgatatcatc gacaccatgc aagtggatac tcagaccgcc
     5101 attcaagtac tgaaggtgga tggtggtgca gccatgaaca acttcctcat gcagttccag
     5161 gcggatattt taggcattga cattgcacgt gctaaaaacc tggaaacaac agctctagga
     5221 gcggccttcc tagcaggttt gtcagtaggg tactggaaag acttggacga gttgaaactc
     5281 ttgaacgaga caggagaact ctttgagcca tctatgaacg aatctcgcaa ggaacaactc
     5341 tacaagggct ggaagaaggc tgtgaaagca actcaagtct ttgcggaagt agacgactaa
     5401 tactggcaga ataaagcgat ttatttagaa agtgtgtaaa tatggaattt tcaaagaaaa
     5461 cacgtgaatt gtcaattaaa aaaatgcagg aacgcaccct ggacctcttg attatcggtg
     5521 gaggaatcac aggagctggt gtagccttgc aggcggcagc tagcggtctt gagactggtt
     5581 tgattgaaat gcaagacttt gcagaaggaa catctagtcg ttcaacaaaa ttggttcacg
     5641 gaggacttcg ttaccttaaa caattcgacg tagaagtggt ctcagatacg gtttctgaac
     5701 gtgcagtggt tcaacaaatc gctccacaca ttccaaaacc agacccaatg ctcttaccag
     5761 tttacgatga agatggagca acctttagcc tcttccgtct taaagtagcc atggacttgt
     5821 acgacctctt ggcaggtgtt agcaacacac caactgcgaa caaggttttg agcaaggatc
     5881 aagtcttgga acgccagcca aacttgaaga aggaaggctt ggtaggaggt ggagtgtatc
     5941 ttgacttccg taacaacgat gcgcgtctcg tgattgaaaa catcaaacgt gccaaccaag
     6001 acggtgccct cattgccaac cacgtgaagg cagaaggctt cctctttgac gaaagtggca
     6061 agattacagg tgttgtagct cgtgatctct tgacagacca agtatttgaa atcaaggccc
     6121 gtctggttat taatacaaca ggtccttgga gtgataaagt acgtaatttg tctaataagg
     6181 gaacgcaatt ctcacaaatg cgcccaacta agggagttca cttggtagtg gattcaagca
     6241 aaatcaaggt ttcacagcca gtttactttg acacaggttt gggtgacggt cgtatggtct
     6301 ttgttctccc acgtgaaaac aagacttact ttggtacaac tgatacagac tacacaggtg
     6361 atttggagca tccaaaagta actcaagaag atgtagatta tctacttggc attgtcaaca
     6421 accgctttcc agaatccaac atcaccattg atgatatcga aagcagctgg gcaggtcttc
     6481 gtccattgat tgcagggaac agtgcctctg actataatgg tggaaataac ggtaccatca
     6541 gtgatgaaag ctttgacaac ttgattgcga ctgttgaatc ttatctctcc aaagaaaaaa
     6601 cacgtgaaga tgttgagtct gctgtcagca agcttgaaag tagcacatct gagaaacatt
     6661 tggatccatc tgcagtttct cgtgggtcta gcttggaccg tgatgacaat ggtcttttga
     6721 ctcttgctgg cggtaaaatc acagactacc gtaagatggc tgaaggagct atggagcgcg
     6781 tggttgacat cctcaaagca gaatttgacc gtagctttaa attgatcaat tctaaaactt
     6841 accctgtttc aggtggagaa ttgaacccag caaatgtgga ttcagaaatc gaagcctttg
     6901 cgcaacttgg agtatcacgt ggtttggata gcaaggaagc tcactatctg gcaaatcttt
     6961 acggttcaaa tgcaccgaaa gtctttgcac ttgctcacag cttggaacaa gcgccaggac
     7021 tcagcttggc agatactttg tcccttcact atgcaatgcg caacgagttg gctcttagcc
     7081 cagttgactt ccttcttcgt cgtaccaacc atatgctctt tatgcgtgat agcttggata
     7141 gcatcgttga gccaattttg gatgaaatgg gacgattcta tgactggaca gaagaagaaa
     7201 aagcaactta ccgtgctgat gtcgaagcag ctctcgctaa caacgattta gcagaattaa
     7261 aaaattaaga aaaaataaaa gaggtggagg gcagcattcc ttgtcgcccg tcccttcttt
     7321 ttaatggaga cagaaagatg atgaatgaat tatttggaga atttctaggg actttaatcc
     7381 tgattcttct aggaaatggt gttgttgcag gtgtggttct tcctaaaacc aagagcaata
     7441 gctcaggttg gattgtgatt actatgggtt gggggattgc agttgcggtt gcagtctttg
     7501 tatctggcaa gctcagtcca gctcatttaa acccagctgt gaccatcggt gtggccttaa
     7561 aaggtggttt gccttgggct tccgttttgc cttatatctt agcccagttc gcaggggcca
     7621 tgctgggtca gattttggtt tggttgcaat tcaaacctca ctatgaggca gaagaaaatg
     7681 caggcaatat cctggcaacc ttcagtactg gaccagccat caaggatact gtatcaaact
     7741 tgattagcga aatccttgga acctttgttt tggtgttgac aatctttgct ttgggtcttt
     7801 acgattttca ggcaggtatc ggaacctttg cagtgggaac tttgattgtc ggtatcggtc
     7861 tatcactagg tgggacaaca ggttatgcct tgaacccagc tcgtgacctt ggacctcgta
     7921 tcatgcacag catcttgcca attccaaaca agggagacgg agactggtct tacgcttgga
     7981 ttcctgttgt aggccctgtt atcggagcag ccttggcagt gcttgtattc tcacttttct
     8041 agtttatact cttcgaaaat caaattcaaa ccacgtcagc gtcgccttac cgtactcaag
     8101 tacagcttac ggctagcttc ctagtttgct ctttgatttt cattgagtat tagaaaacaa
     8161 ttatgttgat agagcttggg caagagccca atttcagcaa aaaatgaagt aaatcttctc
     8221 ataataaaac gcatcatatc aagcacgaaa attccacgag gtcaactaca gtcagaaagc
     8281 tgaacaacaa gccaaaacgc ccaaaaaagg cggcaaaaag caagcacctg caagcaacgt
     8341 gccgaaatgg tcaaatcctg attatgtcaa cgaattagac ccaaaaatcg ttgatatgct
     8401 agtagaattt cacaagtcac aaggcacttt ggaaactccc gaggcgcaag cagaaatcgc
     8461 ccaaaaacgt gaagaaatcg agcaaaggag agctgagctt gaggataaaa aacaagagct
     8521 tttgaaccgc ttgaacaaat agagtttcgc aagtattatg cttacaaatt acttgagcaa
     8581 ttaactaaaa tataaaccct gcctttatat ctaggcaggg tttatatttt agaaattcac
     8641 gtaggttgtt acggttttta catacccagt atagtttgag tttctatagt attcagtgat
     8701 aaacttccat tttttttgag caacatggat ataagtactt gttatgtagt atggatatgg
     8761 gctttgtgaa tccaagtaag actgataagc ttgtatacca aaatatgctc caccaattat
     8821 tgcaccccat ggacccccca ataaagtacc tatcctacca atcatataac tgattccagc
     8881 accagtcatg aagttagcga atgtgttagc ttgtttattc ccatgtattg tgttgacgta
     8941 attccaaaca ttaggatcgt atgatctaaa agatatattt aggtcgattt cattcttttg
     9001 ataagccata taaaatgccc cattgatata gacgccgtca gcacgtcgtt caatagtgtc
     9061 tacacttcca tctggattga caacctcaag aacttcatcg cttaaaatat ttacttgcgt
     9121 atctccgaac cgcactgatg agccattctc aaactgagcc tcaccagata caactttaga
     9181 gtttgccgat aagctatcat cagcaaaaac aaacaagcga cggggaaatg ctagacatac
     9241 agaaaacaga cataactagc aaacacatgc atttaaacat cttagacata acggaaactc
     9301 ctttgtattt ttgatttttt tcaactttta ttatacaata aaaccaaata aaaagaaagc
     9361 ggtaacaata tgcttaatgc gaaaattttt tatatatttt tatgtttgat cgttatcgaa
     9421 actacaggct tgttgttgtt gaaaagaggt ctcgaaatgg gttatttaga cacagaagct
     9481 attatcctcg cagttttttc atttgctttt tacaacctat gttcattcgc ttgggtctgc
     9541 tctacaataa aaaacaataa aaaataaata gacgtatttt caaaaaaaaa aaaaaaaaaa
     9601 aaatgcatat ttatcttagc aaaacgacaa tttaaatcgt cgtttttttg t
//
LOCUS       DAVBCR010000074         9594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCR010000074 DAVBCR010000000
VERSION     DAVBCR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.074"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQ508_001725"
     CDS             complement(<1..947)
                     /locus_tag="VQ508_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490691.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQ508_001726"
     CDS             complement(1027..1890)
                     /locus_tag="VQ508_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2490692.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQ508_001727"
     CDS             complement(1893..3794)
                     /locus_tag="VQ508_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2490693.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQ508_001728"
     CDS             complement(4222..4497)
                     /locus_tag="VQ508_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2490694.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQ508_001729"
     CDS             complement(4603..5442)
                     /locus_tag="VQ508_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2490695.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQ508_001730"
     CDS             5584..6432
                     /locus_tag="VQ508_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2490696.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQ508_001731"
     CDS             complement(6525..7442)
                     /locus_tag="VQ508_001731"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2490697.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQ508_001732"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQ508_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ508_001733"
     CDS             complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ508_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2490698.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(8705..9049)
                     /locus_tag="VQ508_001734"
     CDS             complement(8705..9049)
                     /locus_tag="VQ508_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2490699.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ508_001735"
     CDS             complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ508_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2490700.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(9415..9502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
BASE COUNT         2931 a         2014 c         1613 g         3036 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aatcacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgtacttga gtacggcaag gtgaaactga
     8221 cgtggtttga atttgatttt cgaagagtat caatccacta tacatattaa aaagtatgga
     8281 tatcaaaaca aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     8341 agcttcctca cctttacatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     8401 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     8461 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     8521 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     8581 tgctttagct ccaagacgtt ttgcttgtga atcacgtccg tttgatgtag aacctccacc
     8641 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     8701 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaatagttc tccgataagt
     8761 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     8821 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     8881 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     8941 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     9001 cacccctcag ctcgccatct tcggctctct caaagactgc ttgtatcatg tgttctcctt
     9061 aaaattaagc gttaattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     9121 tacggtggct acctttttta ggtttgtact tgtaagtaac aactttcttt tgttttcctt
     9181 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     9241 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgaccagct tcaacgttca
     9301 atttttcaat gtaaactgct tgaccaactt caactttaac ttgttttccg ccagttttga
     9361 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     9421 gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc aagcaacgat
     9481 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     9541 tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt ctgc
//
LOCUS       DAVBCR010000075         8781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCR010000075 DAVBCR010000000
VERSION     DAVBCR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.075"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ508_001736"
     CDS             complement(135..1103)
                     /locus_tag="VQ508_001736"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2490701.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ508_001737"
     CDS             complement(1248..2063)
                     /locus_tag="VQ508_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2490702.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ508_001738"
     CDS             complement(2088..2585)
                     /locus_tag="VQ508_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2490703.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ508_001739"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ508_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2490704.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ508_001740"
     CDS             complement(4033..4548)
                     /locus_tag="VQ508_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2490705.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ508_001741"
     CDS             complement(4550..4993)
                     /locus_tag="VQ508_001741"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2490706.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ508_001742"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ508_001742"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ508_001743"
     CDS             complement(5180..5647)
                     /locus_tag="VQ508_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2490707.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ508_001744"
     CDS             complement(5848..7134)
                     /locus_tag="VQ508_001744"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2490708.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ508_001745"
     CDS             complement(7365..7601)
                     /locus_tag="VQ508_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2490709.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQ508_001746"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQ508_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2817 a         2097 c         1407 g         2460 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcagta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata t
//
LOCUS       DAVBCR010000076         8602 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCR010000076 DAVBCR010000000
VERSION     DAVBCR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.076"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            218..1408
                     /locus_tag="VQ508_001747"
     CDS             218..1408
                     /locus_tag="VQ508_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2490710.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(1729..2610)
                     /locus_tag="VQ508_001748"
     CDS             complement(1729..2610)
                     /locus_tag="VQ508_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2490711.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ508_001749"
     CDS             complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ508_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2490712.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ508_001750"
     CDS             complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ508_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2490713.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ508_001751"
     CDS             complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ508_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2490714.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(5044..6423)
                     /gene="vex3"
                     /locus_tag="VQ508_001752"
     CDS             complement(5044..6423)
                     /gene="vex3"
                     /locus_tag="VQ508_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2490715.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(6475..7122)
                     /gene="vex2"
                     /locus_tag="VQ508_001753"
     CDS             complement(6475..7122)
                     /gene="vex2"
                     /locus_tag="VQ508_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2490716.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(7135..8412)
                     /locus_tag="VQ508_001754"
     CDS             complement(7135..8412)
                     /locus_tag="VQ508_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490717.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         2439 a         1884 c         1631 g         2648 t
ORIGIN      
        1 acaacctcaa aacggtgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattaagt ataaacagcc actctccagt tcaattggag agttttttga tgagagtaga
      121 tatttataga cctcaatttg acaccataca ttttctatga taaaatgtaa caaattcatt
      181 tacaacaaat cgaaatctaa atgaaatgga attttctatg aaatcagtca aaggactact
      241 ctttatcata gctagtttta tcttgactct tttgacttgg atgaacactt ctccccaatt
      301 catgattcca ggactagctt taacaagcct atctctgact tttatcctag ccactcgtct
      361 cccactacta gaaagctggt ttcacggttt ggagaaggtc tacaccgtcc acaaattcac
      421 agcctttctc tccatcatcc tactaatctt tcataacttt agtatgggcg gtttgtgggg
      481 ctctcgctta gctgctcagt ttggtaatct tgccatctat atctttgcca gcatcatcct
      541 tgtcgcctat ttaggcaaat acatccaata cgaagcttgg cgatggattc accgcctggt
      601 ttacctagcc tatattttag gactctttca catctacatg ataatgggca atcgtctcct
      661 tacatttaat cttctaagtt ttcttgttgg tagctatgcc cttttaggct tactagctgg
      721 tttttatatc atttttctat atcaaaagat ttccttcccc tatctaggaa aaattaccca
      781 tctcaaacgc ttaaatcacg atactagaga aattcaaatc catcttagca gacctttcaa
      841 ctatcaatca ggacaatttg cctttctaaa gattttccaa gaaggctttg aaagtgctcc
      901 gcatcccttt tctatctcag gaggtcatgg tcaaactctt tactttactg ttaaaaattc
      961 aggcgaccat accaataata tctatgataa tcttcaagcc ggcagcaaag taaccctaga
     1021 cagagcttac ggacacatga tcatagaaga aggacgagaa aatcaggttt ggattgctgg
     1081 aggtattggg atcaccccct tcatctctta catccgtgaa catcctattt tagataaaca
     1141 ggttcacttc tactatagct tccgtggaga tgaaaatgca gtctacctag atttactccg
     1201 taactatgct cagaaaaatc ctaattttga actccatcta atcgacagta cgaaagacgg
     1261 ctatcttaat tttgaacaaa aagaagtgcc cgaacatgca accgtctata tgtgtggtcc
     1321 tatttctatg atgaaggcac ttgccaaaca gattaagaaa caaaatccaa aaacagagct
     1381 tatttacgaa ggattcaagt tcaaataagt aatcatactc tcctgggatt tcccaggaga
     1441 gttttttata gccaaatgaa ataaaaccgg aacgatggaa ttttggcatt caaatcaatt
     1501 gataagaata ttttatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa
     1561 atgaatttat agaaatcttt tagcagttat gttgtcctat tctagtttca aaacgctata
     1621 gaagcagcat tgtgctagtc tagattcagt ttactatact aaaacgagta gcttgaaatc
     1681 aaaaaaccca ccctcacagg caggttttat ctgtattatt cagctagatt atgctttacc
     1741 ttctgaaccg aatacgtcga tacgttcttc aaccgatgct tggatagctt ttacaccgtc
     1801 agccaagaat ttacgtgggt cgaagagttt tttcttgtcg tattctgctt cgtttgcttc
     1861 gtagtcacga gcaaatttac gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac
     1921 gttaactttg gcaacaccaa gtttgatagc tgcttggatt tgctcatcag gaatacctga
     1981 tccaccgtgc aatacgattg ggaatcctgg aagagcttct gtcaattttt gcaagtggtc
     2041 aaggtcaaga ccttcccagt ttactgggta agggccgtgg atgttaccga taccagctgc
     2101 caagaagtcg ataccagttt caaccattgc tttagcgtct tcgattggag ccaattcacc
     2161 tttaccgatg attccgtctt cttcaccacc gatagtacca acttcagctt ctactgagat
     2221 acctttagcg tgtgcttttt caacaacttc tttagccaat ttaaggtttt cttcaactgg
     2281 aaggtgtgaa ccgtcaaaca tgattgaagt ataaccaact tcgatacact caagtgcatc
     2341 ttcgtagtga ccgtggtcaa ggtggatagc tactggtaca gtgataccca ttgattcaac
     2401 aaggttagcg atcaagttgc gagcaacttt gtaaccaccc atgtatttag cagcacccat
     2461 tgaagtttgg atcaaaactg gagctttttt agcttctgct gcgcgcaaga tagcttgagt
     2521 ccactcaagg ttgtttgtgt taaatccacc aactgcataa ccgttgtcac gggctgcttg
     2581 gacaaatttt tctgctgaaa cgattgccat tttatcaggc ctcctgtata tttttatggg
     2641 tcatcccatt tacattgttc attttatcac tttttgccaa aaaaatctag tttttcccgc
     2701 agtttcgatt gattttcttc taactccatc tatgtaaacc ctttctctcc ctagtcttgg
     2761 acgacttttg gaaaatctat aaagaaggtt aaacgattct cctccatctc gaaacgataa
     2821 gctaattttt catgttctaa tagactctta accacaaaga gccccatacc agaccccttg
     2881 accttgcgac tggcattgtc agaaaaagac tgggctagtt tttcttgttc ctctgagcta
     2941 cagctatttt cgataaaaag ttctccttct ctttctccaa ttcgaactaa gccacctgga
     3001 acagagtgct taatggcatt gctgatgaga ttagaaagaa tcaacttcat aactgatggg
     3061 tttagataag cctgctgatg ggtcaaacta ttgtctatct ggagctctct ttccttggct
     3121 agcaaggcat aatctttgac cagattttgc gtcatctgga ggaggtcaat tgtttcccta
     3181 tcatctcgca attcctgcac agaagagagg gaaagtatct gcagaacatg gtgattgagt
     3241 tcatccacaa tccccaaggc aactcccaga tactggtctc tatccttata acgaccgata
     3301 ttctctctca tattttcgat taggattttc aaactagcca gcggtgtttt caattcatga
     3361 gaagctcctc gtaggaattc gaccttcatc ttctccagct ggagaatggc ttcattcttt
     3421 tcatgcaagt ccgcaataac agtcaagaga tgctggtaga ggctattgat ttgttccttg
     3481 agattaccta tctcatcctt agaatccacg cgcaatcgca cttgggaatc caggtccatc
     3541 atccgacggg tcacccgctt gatttccaag atcggtgcaa caatagtccg agcgtagatg
     3601 taggccacca agagggaaat cagaaaggag gccagcaagg tatagggaag aaactggaga
     3661 ctgatttgct ccgcttcctt ttgtaaatcc atggaagcta gaaactggag aatcatagta
     3721 gcaccgtctt gcgttttcac ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct
     3781 gtgtccagag gaagactgtc cttgacttct aacttgtcct cggtcatctc acctttgacg
     3841 gtccccttga tatcactagt ctgggaatac aagtctaaca cttgctcgat actctgccta
     3901 tctttccctt ctagggactg ggcaatggct gttgcctttt gaccaatggt ttcctgacga
     3961 tgactcagat aagtcgaagg aaaaagaaaa taaatagcta aatgaaggca gataaccaga
     4021 acactaaata tcgagaaggt atagataaat atctttgcaa ataaacctgt tcgtttcatt
     4081 ttcgctccaa tttataacca acattgcgca cagtgaggat acaatccaag tctagctttt
     4141 tccgcaattc cttgatataa acatcaataa cacggtcaaa gggaacctca tctgtcgctt
     4201 tccagacggc atcgataatc tgagatcgag tcaaggcccg gccttcattt ttcactagat
     4261 agtccagaat ttccaactct ttggcattga taggcacttc ttgacctgcg atgcttgcac
     4321 tgtagctttc aaagtccacc ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt
     4381 agcgcttgaa aatcgcgtcc accctcactt ttaaaaggga gagggagaaa ggtttttcca
     4441 gatagccatc tgccaaagag gcaaaggcac tcatcttgta ttcctcatct tgaaaagctg
     4501 tcaacatcaa gacaggaacc tgactggttt tacgaatctc agctaggact tctaagccgt
     4561 tgagcttggg catctggata tccagtaaaa ccagggccac ctcatagcta gaaaattgct
     4621 ccagagcttc ctgaccgtcc gctgcctcaa tagtttcata gccacaatcc gtcaaataat
     4681 cactgacccc ctcacggatc atctcttcat cttctacaat taaaattttc atactttaac
     4741 tgctctctat tttttatttt tcttagaata aatacctact ctattttcta ttatagtctc
     4801 ttgctggcct tttgtctgca agcaactgac cactagataa aacgttgtga aattcctttc
     4861 tcataaattc cataacttta gtatattata tttaagcact aaagtacaaa gaaagcaact
     4921 gaaagcaatg attttcacca ctgctttcgg atttattttg aattgttaaa tagccattcc
     4981 tatccactat tcttgaatag aaacacaaga tgcaatcttt attctagact cattttttca
     5041 aatttattca ccatccagca agagctcttt tggttgtttt ctaaggagat tgcttgaagc
     5101 aagcgccata acgagaacca ctagaaccaa ggcaaggaca aaaatgatga taaagtctga
     5161 tgtctgaatg gaaatgtcta ggctcgacaa ggtcttgcta aagccatcta cttctgcacc
     5221 accaccaagg ttagaggctt gagccgcctt actagcctgt ttggcaacac ctgaagtcac
     5281 attggcaagg acagtgtttc caattgcacg ggcagtgtaa ttagctagga agtaagcaga
     5341 aactagagca gggatagcaa tcaagataga ttcggtgatg aattgaccca agatacttgc
     5401 ctgcttgagg ccgatagaga ggagaattcc cacttccttg cgacgggcgt tgatccaaag
     5461 gctgagcaag agggcaagga ggagaactga gaagctcaag ctaccccaga agaggaggtt
     5521 ggccatcttg tacataccag agatagattg ctcaagagct gggtagttag aggagctctt
     5581 gacgagtgtg tagctcttcc agttgatacc actgatgcca ttcaactctt tcataacatc
     5641 atccaagttc ttgtctgctg ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc
     5701 tgtgtatcca taaagttttg cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag
     5761 ttcttgtgag taggttactg ctgacttatt atgaccatca aagagtccct tgattgtcac
     5821 ttcaactgtt tccttggctc ctttttcatt atctgcatcg tagatattag agtccagttt
     5881 aaccttgtcc cctactttcc agccgtgttt ggctgccaag tccttgtgca agaggatttt
     5941 atccttgtcg tcgttggtta agtgctctcc ttcgactagt ttataagaac cagagacaaa
     6001 cttgtcttct ttagaggagt cattgacacc tgtaatcatc aagctacttc caaaacgctt
     6061 ggcacgatca gcagtgagat tcttcttggt ttctggcgtt tcaatcaggt catatccagt
     6121 caaatctccg atagcgttga tacgtttgac ataagactcg atggccttgt tttcggtgat
     6181 ttttttgatg tcttcaccct tgatattccc agcaccacga ggcgttcctt ggttgacgcg
     6241 acgattgatt tgcatggaga agctattagt gatattttta aaggtctcct gagaagcctt
     6301 ggcagtagct cccttgattg acaagccgac caaactcaag ctcgccatga ggagaataat
     6361 caggaagatg acaatcgatt tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa
     6421 catagattcc ctttctagat tttgttttaa tcattctatc aaaataagct caaattattt
     6481 actagtattg cgcgtttcag tcagtttctt atcctttaat tcaagtgtaa tatctgacgc
     6541 ttgtgccact tctttactgt gagttacgac aatcacacat ttacctgttt tctgggcaag
     6601 tgatttgagt agttcgacaa tatctccagc agttttagga tccagatttc ctgttggctc
     6661 atcagctaga ataactggag cttctgagac caaactgcga gcaatggcaa cacgttgctg
     6721 ttgaccacct gataactgga gaacattccg cttgatctgg ctttcatcca aaccaagctc
     6781 aagaagtgta tccttgcttg cctttttgtt gaccaagcgg atattttcca gcggagaaag
     6841 ataatctatc aagttataat tttgaaagac cagggaaata tggtgcatgc gatggtaaga
     6901 atagcccttc ttacgaatat cctctccttg aaaaaggata gaaccttcaa caggactatc
     6961 tagaccagca agtagggaca agagtgtgga ttttcctgct cctgactccc caataatact
     7021 gtaaaatttt ccaggttcaa aattataatc gatctgatat aggactgctt cagcagtatt
     7081 cttataacgg taggtaacat cttgtaattg taataaagtc atgatttctc cttcttaact
     7141 aatagatgat aaaatttctt tcggtgattt tctaaataag aataggaaac aaagggctac
     7201 agataagcaa ctaagcagaa ctagaaaaac ataggattct gcaaaagata agatgctagt
     7261 tgataaactg cttgctttgg ctagtgtatc ttgtaagctt gcctgatctc cacttgctag
     7321 tagagtttgg agtagataac ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc
     7381 tccaagagat accaaaacta cctctaaaca gaattgtagg aagatcgagc tcttgccttt
     7441 tccaagtgca agtaaaatcc ccacttcata gacccgttct ctcaaccaga gagacaaaac
     7501 cagaattaag gctccagctc ctgctatcaa catcccataa aggaagatgg tcaagaaggt
     7561 ttggaaagtt gcaactgagt ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg
     7621 gtagccttga ttttccaagg ccaagttttc tacctgcttc atgagtccgt ccatttcctt
     7681 aggattttct acatagaagc gtgctgcact gacttgagct tcactattgc ccaaaagggt
     7741 ttggctactt tcatagtctg taaagacttg attttcactg aagtcagaag acaagcctgt
     7801 gaatttctct tgttttttac cagaaaagat gccgataatc tcaaactcta ctgtttgtcc
     7861 ttttccagat tcagactgac cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt
     7921 cttcttagcc aattcttcgt ggataaggat tttcttggaa tccccttttt gaaggtgtcg
     7981 cccttctttt agattgaaag ccgaactggt aaaggttaca tccttggatg aatcctcaag
     8041 agccgttaag ctaaccaagt tattgtctgc agctgataaa tcatcacgct ccacgctctg
     8101 ctcaccagtc actgcttcct tgtcttttag ttttgcgacc gtctcaagtt caggagagac
     8161 attttccagc cccttaatct tgcttacaga tgctaggtct gacaacttga atgtctgacc
     8221 attctctatc ttcttaatag aaaaagatgt attgagtgat ttataaagat tgctttctac
     8281 tgttttgttg gacttcatca gagtcaaaca ggctgaaatt ccggccaata agaccaataa
     8341 aatcagaaat aaaataaaac ttctcagtcg ctttctgctg acataagccc aagatctttg
     8401 gattggattc atttgtcacc tccatatttg taagactatt ataaaaccca aatatgaaat
     8461 gtttatgaaa tacgaaaaaa aatatcgagt aggggataat ctctagcccc tctcacacca
     8521 ccgtacgtgc cgttcggcat acggcggttc aactaacttt taacgcatgt cgttcaaggt
     8581 aataatccaa acacgaaacc tg
//
LOCUS       DAVBCR010000077         8559 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCR010000077 DAVBCR010000000
VERSION     DAVBCR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.077"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            221..2830
                     /locus_tag="VQ508_001755"
     CDS             221..2830
                     /locus_tag="VQ508_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2490718.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2863..4803
                     /locus_tag="VQ508_001756"
     CDS             2863..4803
                     /locus_tag="VQ508_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2490719.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4800..6407
                     /locus_tag="VQ508_001757"
     CDS             4800..6407
                     /locus_tag="VQ508_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490720.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6422..7162
                     /locus_tag="VQ508_001758"
     CDS             6422..7162
                     /locus_tag="VQ508_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490721.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7165..8484
                     /locus_tag="VQ508_001759"
     CDS             7165..8484
                     /locus_tag="VQ508_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490722.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         2949 a         1060 c         1602 g         2948 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
      121 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
      181 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
      241 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
      301 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
      361 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
      421 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
      481 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
      541 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
      601 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
      661 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
      721 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
      781 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
      841 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
      901 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
      961 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
     1021 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
     1081 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
     1141 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
     1201 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
     1261 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
     1321 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
     1381 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
     1441 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
     1501 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
     1561 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
     1621 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
     1681 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
     1741 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
     1801 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
     1861 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
     1921 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
     1981 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
     2041 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
     2101 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
     2161 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
     2221 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
     2281 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
     2341 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
     2401 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
     2461 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
     2521 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
     2581 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
     2641 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
     2701 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
     2761 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
     2821 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
     2881 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
     2941 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
     3001 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
     3061 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
     3121 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
     3181 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
     3241 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
     3301 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
     3361 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
     3421 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
     3481 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
     3541 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
     3601 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     3661 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     3721 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     3781 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     3841 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
     3901 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
     3961 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
     4021 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
     4081 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
     4141 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
     4201 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
     4261 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
     4321 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
     4381 ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
     4441 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
     4501 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
     4561 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
     4621 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
     4681 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
     4741 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
     4801 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
     4861 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
     4921 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
     4981 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
     5041 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
     5101 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
     5161 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
     5221 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
     5281 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
     5341 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
     5401 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
     5461 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
     5521 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
     5581 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
     5641 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
     5701 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
     5761 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
     5821 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
     5881 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag ccacaaaaag
     5941 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
     6001 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
     6061 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
     6121 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
     6181 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
     6241 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
     6301 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
     6361 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
     6421 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
     6481 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
     6541 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
     6601 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
     6661 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
     6721 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
     6781 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
     6841 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
     6901 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
     6961 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
     7021 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
     7081 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
     7141 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
     7201 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
     7261 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
     7321 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
     7381 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
     7441 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
     7501 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
     7561 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
     7621 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
     7681 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
     7741 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
     7801 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
     7861 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
     7921 tctataatta tatttagaaa taattttatg ttcattgctt cgaagtggaa tttactatat
     7981 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
     8041 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
     8101 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
     8161 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
     8221 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
     8281 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
     8341 tctttagagg atgttttaat agatgatatg aatcttttgg ggttaatact tccaaatttt
     8401 atagtgtcta ttattttatt tattttaata tttattacta aaagttccta ttttatcttt
     8461 aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa atagttaaat
     8521 ctattttgta tcattgcttg cattttactg gtggagaga
//
LOCUS       DAVBCR010000078         8369 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCR010000078 DAVBCR010000000
VERSION     DAVBCR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.078"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(626..934)
                     /locus_tag="VQ508_001760"
     CDS             complement(626..934)
                     /locus_tag="VQ508_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2490723.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(931..1239)
                     /locus_tag="VQ508_001761"
     CDS             complement(931..1239)
                     /locus_tag="VQ508_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2490724.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(1244..2539)
                     /locus_tag="VQ508_001762"
     CDS             complement(1244..2539)
                     /locus_tag="VQ508_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2490725.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNTVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2557..3966)
                     /locus_tag="VQ508_001763"
     CDS             complement(2557..3966)
                     /locus_tag="VQ508_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2490726.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4124..4852
                     /locus_tag="VQ508_001764"
     CDS             4124..4852
                     /locus_tag="VQ508_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2490727.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            5312..5506
                     /locus_tag="VQ508_001765"
     CDS             5312..5506
                     /locus_tag="VQ508_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490728.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(5872..7182)
                     /locus_tag="VQ508_001766"
     CDS             complement(5872..7182)
                     /locus_tag="VQ508_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2490729.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7419..8291
                     /locus_tag="VQ508_001767"
     CDS             7419..8291
                     /locus_tag="VQ508_001767"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2490730.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         2606 a         1470 c         1587 g         2706 t
ORIGIN      
        1 gatagagttg ttcacatctt atttcaattc actatagttt aatttaagag tagtatttac
       61 taaggcccaa ttaaaatcaa agagcaaact agaaaacgag tgccattcag ctcaaaacac
      121 tgatttgaga ttgcagataa gactagcccc tcattaacag gtttacgata aaacgatgac
      181 aaggtgtgtt gctttttgat ttctaaagag tataatgata gatctctata aaataagtgc
      241 gaaggaaatg agcttttata gtcctttcgt tttaaaatac tatctcagat gttcttatat
      301 cgacaagaag tttttgagtc attccctcat catacatatt aaataaatag tggctcattc
      361 aattttttac tagaataata agctagtata gtaaactgaa ataagatata aacaaataaa
      421 ttggagctta acatctattt ccagcaattt tttagaaact gcagtggact attctaaatt
      481 caacacatta taaaaactag agtaaaagaa aaggattgga tcttgtgtaa tgcaggatct
      541 aatcctttca atcattttgt ccaacttttg gaggttccta caatgtagcc gtcattaata
      601 aagacagatg ggaacgacag tgttcctatt tattttgata gagatcgatg aattctttag
      661 atagcaactg aataatctct gttgaagcca tttggtcttc tgcatgcata aatagcaagg
      721 agaatcctat tttttctcca gtagcttctt tttgtatgag attagagtga atcttgtgcg
      781 cttctactaa ggagtcttcc gcttcttcaa ctttaatttt cgcttctttt aaatttcctg
      841 ccttagctag ttggatggct tcaataaagg atgatttggc tgctccacta ttggcaatga
      901 gctgaaaaca gatatattcc atttcttctg tcatcttatt tctcctatcc atgcaagtgc
      961 ttgttccaga acttttgctc cattcatcat tccgtaatcc cgcatatcaa tggtatctac
     1021 agggatattt cctgcaattt ctttcacagc aagtaactca taacgaattt gtggcccaat
     1081 tagaatgaca tctgcttcat ggatattctt tttcgcttct gtcattgatt ttgcttggat
     1141 agaaatttca atcccacgtt cagtcgcact ttgttgcatt tttttaacaa gcatacttgt
     1201 cgacattccc gcattacata ctaataaaat ttgtttcata atcttaacct tccatttctt
     1261 gctcaacaac tttgtcatta actttgataa atggaatgta tagaagaact ccaagtgcaa
     1321 agatgatgaa ttgaactaga actgctctca cgtcccctgc tgttgctaac catgcattta
     1381 agaatactgg tgtagtccaa ggaacttgta taaatgcagg actcatgaat cctgtaactg
     1441 ttgctaagta gctgattaaa ataccaagga ctggaactgt gataaatgga atagctaatg
     1501 aaatgttata aacgattggg taaccgaata atactggttc attgatattg aagataccag
     1561 gtccaaaaga taatttagcc acgtttttag aaacagtatt gcgactcact aagaatgttg
     1621 ctattaataa acataatgta gatccactac cacccattaa agcgaatgtt tgtatttgtg
     1681 ataagttgat gatgtgtgga atggcttgtc cattatttgc tgcagtgatg ttttcagtaa
     1741 tgttaattaa tagtaatggt tctaggatgg cactgtaaat aactgcttgg tgaataccaa
     1801 atagccataa catatttcct aaagagtaaa taataatgac cccgattaag cttgtaccaa
     1861 tatgacgaat tggttcttga ataaagattg taatgattga gattaagttc attccagtta
     1921 tattgaataa taatgctgaa acaatcccaa ataaggagat gacagtcatg actggaagta
     1981 atacgctaaa tgatctacta acagctggtg gaatattttc accaaggttc atttgtaaag
     2041 ctttaacgtt tgataattca atgaataatt ctgttgcaat aatcgctacg ataaccccgg
     2101 cgaacattgc gcctgtacct gtgttgttga atgaaagaac acctgaaatg tttacagcat
     2161 cttttgctcc gtcaggaact acagaaactg tatttggcat catcacaatt aaagaaacta
     2221 atgatagcat tgatgctgct gacgggtttt cgaaatctct gtttttagct aagaaataac
     2281 caaccattac agcaataatc atacctgaaa tacttaaagt accgtttgca attgttattc
     2341 cccaatattg gaattttgtt aatgtatccc cttggaaaat ccacttaaat accgtgttgt
     2401 tcaaaagaac gattaaacct gccaaaatat ataatggcat tactgttacg aatgcatctc
     2461 ttagggtttt taaatgaatt tggttcccta gtttaccagc aaaggtggca aattgatcta
     2521 taaattttga gtttttcatc tgaatatctc ctctctttac tcctcaattt ctacttcaaa
     2581 accgttattg tcacttactt tgcgatacca tcttcctgag ctcttgatgg ttctcttttg
     2641 cgtttctaaa tcaacggaga taaatccgta acgattctta tatgcattat tccaagacca
     2701 gcaatcaaat gcggtccaag cgtggtatcc aaaacagaga cttccctctt caatggcttt
     2761 atgtagccat cttaaatgtt cttcataaaa ttcaatacga tacacgtcat cgataactcc
     2821 attttcatcg ataaaccgtg cttcgttttc aacacccatt ccgttctcac tgataaacca
     2881 tgggatatta ccatattctt ccttcacaat catagcaata tcataaatgg ctttcggaaa
     2941 aatttcccaa ccacgatatg gattcatgcg acgctccggc cattcatact ctttgaagta
     3001 ttggtctggc atccagggcg tctgatattc ctcaggattt gcttgtgctt gaacacgttt
     3061 tggatggtag tagtttactc caagaaaatc aaccgtattt gatttcatca gttgaagctc
     3121 tttttcggta tgactccata acacgccatc tctctctagc tgttttacca atctttctgg
     3181 gaaagttcct ttaacagctg gattcaagaa gactttgtta aagaagtctt ctgtaaatcg
     3241 acttgcttct aagtcttctg gagaattact tcttggataa gcaggtgtca agtttaaaat
     3301 aatcccaatc tttccatcaa gtcctaatga gcgatatagt tgaatcactt ttgcactagc
     3361 aaggtttaga ttatagatga cttgtacggc ctcttttccc tttcctttta gatttggata
     3421 atggaaagca tataagtatc ctgcttctgg aatgaccatt ggctcattga aagttgtcca
     3481 gtaatgaacc ttatctccaa agcatgtgaa agcagtcttg gcaaacttca cgaataactc
     3541 cactacatgt ttgctttccc aaccaccgta tttttgaaga agttccactg gtaaatcaaa
     3601 atgatgtaaa ttcatcacaa gatccatctg gttcttttta gcttcttcaa tgatggcatt
     3661 gtagaaagca ataccttttg gatcaggctc acctgtctct aaattcttga tgagtcgact
     3721 ccattggatg gaagtgcgga aagaattaac tccaatttcc ttcatcaagt ggaaatcttc
     3781 ggtgtaagta tgaaaaaagt tactggctac aagaggtccg acattatcga aaaaatcttc
     3841 tggatgcgtt ttgaaccagt agtccatcac attttcatgt acttttccat attgaccttc
     3901 tgtctgaggt ccgctagaag cagctcccca ccaaaacaga ttaggaaata ctaacttttt
     3961 tttcatgctc aacctccttt ctattatgct atacataaag tatagcataa ttttaaaatt
     4021 tgtctagaca tttttaaaaa ttgttgatac atatattgtg tttgcctcat ttcttgaact
     4081 ttgttatact actattagaa tattatagag agaaccagat taaatgagta caaaatattt
     4141 atttatttac aatgagattc gtgaaaagat tctctgtaat aaatatacca tgaacgaaca
     4201 attgcctgat gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa
     4261 agctttagac ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt
     4321 tgttctctct cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg
     4381 actgacaaaa atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa
     4441 attagaattt gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt
     4501 ttataatatt taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta
     4561 tatgagtacc gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa
     4621 ttacattgaa ggaaagttag gattgcatat tgccagtgct acaaaaatct tgcgagcttc
     4681 ttctagttca gaaaatgagc aacattactt gcagctcctt ccaacagaac cggtatttga
     4741 agtagaacaa gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca
     4801 tcgctatgat ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat
     4861 gtgaaaatga agccaatctt ttacaggctc tagtttaaga aaaatttaaa acagggcaag
     4921 aaggtcccat ctatgcttaa atggtttctc ttttctaaat aagatggctt taaaagagtg
     4981 atcgttttat ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta ggtactgaaa
     5041 ttgttcacct gatctacttc ttatagttat ttagttttaa atagtgtttc aaacattctt
     5101 acactgacga gaagtttttg agtcttttct tgtaacacat atagtatact gaggttagaa
     5161 tagtagactg tgacttctaa caaattgcta gaaatgaatt tcaatctccc aatttatttg
     5221 cttatatatt attttaatat attaaataaa ttctggatca taatcattta aaaaaatttt
     5281 atttttttat ttttcattac gaataatata gatgaagggg aaagagtatg aaaacagaac
     5341 tgtttctttt gctattagtt caaaaggaga aaaaatgaaa gtagaaaata tttcgtatag
     5401 ggtggatcat cgtatattgt ttgataatat ttcttttgat acttcgagtt cagacgtgac
     5461 attaattact ggtaaaaatg gtacaggaaa gtcaacttta ctatagtgga ttgaaactag
     5521 aatagtacac atctacttct aaaatattgt tagaaatcga tttgactatc ctgatctatt
     5581 tgtcctgttc ttatttcatt tcactatatc tcaaattgag tatgacgaag tgcgctccca
     5641 tgtcctggga acgcactttc ttcatatttt tcatattctt gaatccatcg ataaagacta
     5701 ttgggatgaa tttttaaagt tgaactaatc atttttacag gatgaggttt acagcagaga
     5761 gtttgaaggc tttatcaaag gtttttcttg gcataatgac ttttcctcgt ttccacttaa
     5821 ttttgtgtct actttattat accaagtcca cccttaagtt agataataaa tctaacttaa
     5881 ggaagctaga aggatgagaa tccaggtggt caagagtccc aaacttaagc tgattgggac
     5941 acccagaata atttgctttt tgaaggcaag gccacgttcc tctatattgg gaagtgagag
     6001 ttgaatgaga gaaccagctg atgaaaaggg tgagatatta gtagatagag cgccaataac
     6061 ggtggctgtt gtgagtaagt gaatatcaat ctggggattt tgagcactga tgatagcaat
     6121 gatgggaaag agggctggag ctacaacgga tagggtggaa ctaaagagtg acatcacccc
     6181 ggctatcaca caaaagaaca gaggtaacca gaaatgagga atggatgttg tcatgaggtg
     6241 ccctatcagt gtgactaaac ctgacttgac cgctagagac attagtaagc tcatgccgca
     6301 gagcatgata attgtagccc agggaacttt agccaaaatg gcttcttgct tccctaattt
     6361 gagccttaag gcgaggcaga ccatgagtat tgagacaaag ccaatatcaa atgtttttcg
     6421 ataagtagct atccaggcga tgtttgggaa aatgagatgc aacaagggaa aaagccaaac
     6481 caaaaccatg ctgctgatca tgagcaaggt ggtttgtctt tgaaccttgc tgaggagtgg
     6541 tggttggtca atagtcaagg atgagtttgt tcttccctta ctatagtgac tgtaacagga
     6601 taataaaagc aagacaatga gtgggtagat aatgctgacg ataaagatat gattgccaag
     6661 tgaaaaagct tgctcttccc atcccatttg cttaaacagg ccttgaaaga caatgcctga
     6721 gctactggtt atcaaattag cccctcctga agctccccaa ttgacggctt gagctccaat
     6781 caaagggtgt ttgtccgctt tttgacagag agtaatcgct agaggacagc aaacggccat
     6841 agtagtgaaa aatccagcac ctaaagcaga caaaagggtt gccatcaggt ataaaatcat
     6901 gtagagggcg ttagggtagg tgcgtgtgcg gtagagaatg tgttgagcca aaacatcaag
     6961 agtaccgtta gttattgcaa agttataaaa gagagagacg ctaaaaatgg taaaaaagag
     7021 tgaggttggc caaaaatgaa gaagttcttt ggggcttaat cccatgagag tggttgcgat
     7081 gaggtaagaa aaagcaatag ccagcaggcc aatattgatt ttggtgcggt aaccaattcc
     7141 aatggctaga gcaatggcgc taatcattat taaatgaatc attgaattgt cctttcagtt
     7201 agaatatgga aataggttgg caattttggc gctcaaaaga ccacctgttt cctattggta
     7261 cttgactatt atacacaaaa aaaatacaat tgtcttgaca attgtattga cacttgttag
     7321 aattaaaaat gaggtattca ggcttgtgat ttcctatgga agttaatagt gattgcctct
     7381 aatgcttaca agtgatatta aaaatagagg acctagtgat gtcaatcatt tcaactgatt
     7441 taaccccttt tcaaatagat gatacattga aagcagcctt gcgagaagat gttcattccg
     7501 aagattacag taccaatgcc atttttgatc atcatggcca agccaaggtg tcgctttttg
     7561 ccaaggaagc tggtgtttta gcggggctaa ccgtttttca aagggttttt accctatttg
     7621 atgccgaggt gaccttccag aatcctcatc aatttaagga tggggatcgt ttgactagtg
     7681 gcgatttggt tttagaaatc ataggctcgg tgagaagtct cttaacatgt gaacgcgttg
     7741 ccttgaattt tttacaacat ttatcaggga ttgcttcgat gacagctgct tatgtagaag
     7801 ccttaggcga tgattgcatt aaggtatttg atactcgaaa aactactcct aatttacgtc
     7861 tttttgagaa atatgcagtg agagttggcg gtggctataa tcatcgcttt aatttatcag
     7921 atgctatcct gctaaaagac aatcacattg cggcagtagg tagtgttcaa aaggcaattg
     7981 ctcaagcgcg tgcctatgcc ccttttgtga aaatggtcga ggtggaagtg gaaagccttg
     8041 ctgctgccga agaagctgcg gcggcgggtg ctgatattat catgttggat aatatgtcat
     8101 tggaacagat tgaacaggcc attaccctaa ttgcaggacg ttctcggatt gaatgttctg
     8161 gaaatattga tatgaccact attagccgtt ttcgtggttt agcgattgat tacgtctcca
     8221 gtggtagttt aacccatagt gctaagagtc ttgatttttc catgaagggg ttaacctacc
     8281 ttgatgtcta agttgtaaaa taaactaact ttttaaagga tgtctttcct ctagaacgag
     8341 ttttatgtca gatagtttaa acgcctctt
//
LOCUS       DAVBCR010000079         8286 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCR010000079 DAVBCR010000000
VERSION     DAVBCR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.079"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..215)
                     /locus_tag="VQ508_001768"
     CDS             complement(<1..215)
                     /locus_tag="VQ508_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2490731.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAA"
     gene            complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ508_001769"
     CDS             complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ508_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2490732.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ508_001770"
     CDS             complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ508_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2490733.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1499..3652
                     /gene="blpA"
                     /locus_tag="VQ508_001771"
     CDS             1499..3652
                     /gene="blpA"
                     /locus_tag="VQ508_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET2490734.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3663..5024
                     /locus_tag="VQ508_001772"
     CDS             3663..5024
                     /locus_tag="VQ508_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2490735.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLNQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5081..5209
                     /gene="blpC"
                     /locus_tag="VQ508_001773"
     CDS             5081..5209
                     /gene="blpC"
                     /locus_tag="VQ508_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2490736.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(5280..6620)
                     /locus_tag="VQ508_001774"
     CDS             complement(5280..6620)
                     /locus_tag="VQ508_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2490737.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEGSQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(6634..7371)
                     /locus_tag="VQ508_001775"
     CDS             complement(6634..7371)
                     /locus_tag="VQ508_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490738.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7376..7714)
                     /locus_tag="VQ508_001776"
     CDS             complement(7376..7714)
                     /locus_tag="VQ508_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490739.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7991..>8286
                     /locus_tag="VQ508_001777"
     CDS             7991..>8286
                     /locus_tag="VQ508_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2490740.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKT"
BASE COUNT         2718 a         1746 c         1304 g         2518 t
ORIGIN      
        1 gctgcatagg ctacacctcc aagtatagct ccacccgcag caccagttgc tgcaccttgc
       61 catgttcctg ttttaatgcc tagttgaaga cctcttgctg ctgctcctcc aacacctgct
      121 ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat ttcagtatcc
      181 ataacagaaa attgtgacat catttttgta tccatgacaa atactccttt tttaaaaaac
      241 taaaataaat cagaatagaa tcctcataat tttactataa gtcttaccaa cttagtccca
      301 atttatcacc aaccatacct cctaagcatg ttaatccacc cccaattgca ccaatgtgtg
      361 ctccaacaaa tgcaccagca agtccagcta ctcctaaagt ggccaaacct gctccagttc
      421 caccagttat aattcccgta gtgactcctg taatcagtgc attttgacaa tcagtggagc
      481 tatacccccc ttcaactttc gcaagcattt cagtatccat aacctctaac tgtgacaaca
      541 tttttgtatt catgatgaat acctcctttt tattttcaat ttgttaccaa agtcttaaat
      601 tcaataaaca aatagatttt ttatagtatc tttttgattt tcttaaaaaa gtatatacgt
      661 ctactatctt cttaaaggta gcagtaccta ttttttagtc taagatttca ataatcttga
      721 gtatctaaaa tatcttaatt tcgttattct ccttgcaata aaaagtttta ctatactatt
      781 tattaacttg cagaaagcaa aaaatattag taaataatag tttatagtta agttttttat
      841 tcctaccaat ccatcaacta agtaaaacat caacgattac ataaacgatt gataatataa
      901 ttaaaatttt gctaactatc ttattctcat cattcttaga taactttgat attttgtaag
      961 taagtaaata agacagtaaa ttaatagcga taataatact atatttaaga atcataatct
     1021 tacaaagagg acataattcc tgaacctaca caaataagtg ttgctgctcc cccagttatc
     1081 ggaccagtcg cagcagctaa tagtactgct ccaatacaac caccgattgc agatcctaaa
     1141 ttgcctcttc ctccactaac tatttcgagt tcttcattat ccataacaga aaattgttcc
     1201 atcatttttg tattcatgac aaatactcct tttttctttt tttatttttg tcttgttgta
     1261 actttgataa gtttagtata tcatcgtttt ttaaaatttt tcatccagat cttgaattgt
     1321 catcgaaacg tcttgaatta gcttttttat ttcaagccac ctctaaatgt ttaaaaaaaa
     1381 taatttctaa tcactttttt accattcagg aagttttaat gactattcaa gatttcataa
     1441 aatatgaact tagttttatg acataataga cctatccact atatgaaaag aattgccaat
     1501 gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg gtgtcgctgc
     1561 cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact tgagagaact
     1621 tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg ctgatgaaat
     1681 gggctttgaa acaaggcctg ttcaagcaga taaaactctc tttgacatga gtgatgtccc
     1741 ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact atgttgtcta
     1801 tcaaacaaag aaagactatc tggttattgg tgatcctgac ccttctgtaa aaatcactaa
     1861 aatgtcaaaa gaacgctttt tctctgaatg gactggagta gctatttttc tagctcccaa
     1921 acccagctat caaccccata aagataaaaa gaatggtcta ctaagcttcc ttcctctgat
     1981 tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattgg tcactattat
     2041 caatataggt ggttcttatt atctccaagg aatcttagat gaatacatcc caaatcagat
     2101 gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc tccaacaagt
     2161 catgagcttc tccagagatt atctcctaac cgttctgagc cagagattaa gcattgatgt
     2221 gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg cgacacgtcg
     2281 tacaggagaa atcatttcac ggttcacaga tgctaactct attatagatg ccttggcttc
     2341 taccattctt tctcttttcc tggatgtttc tattctgatt cttgtagggg gcgtcttact
     2401 ggcacaaaac cctaatctct tccttctttc tcttatttct attcctatat acatgttcat
     2461 catcttttct tttatgaagc ctttcgaaaa aatgaaccat gatgtcatgc aaagtaattc
     2521 tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa agtcgctcac
     2581 gagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt tggaaaaatc
     2641 ctttaagctc agtaaatatt ctattttaca aacaagttta aagcagggaa caaaattagt
     2701 tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta aaatttctat
     2761 cggtcagctg attaccttta acacacttct ttcttacttt acaactccta tggaaaatat
     2821 tatcaacctc caaaccaaac tccaatctgc gaaggttgct aataaccgtt tgaacgaagt
     2881 ctatctagtc gaatctgaat ttcaagttca agaaaaccct gttcattcac attttttgat
     2941 gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag ataccttaac
     3001 agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag ttagtggttc
     3061 tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca aaggacatat
     3121 ttccatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc gtcatattaa
     3181 ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact taactttggg
     3241 cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag ctgaaattcg
     3301 tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg gagctggtct
     3361 atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta aatctcctgt
     3421 tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa aggttataga
     3481 taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc tcagtatagc
     3541 cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag ttggtagtca
     3601 ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat aaggagaatg
     3661 tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac cataactatg
     3721 cgacagtgtt aattatacct ctttcattac tatttacttt catcttgatt ttctcccttg
     3781 ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca agtgtcattg
     3841 cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg gcaaatcaag
     3901 tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa agtcagaaaa
     3961 ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt ggaattttga
     4021 aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt ggctaccata
     4081 atacctttat gaattttact aaacaatccc atgatattga actgggtatc acaaagacta
     4141 acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct attgaacaag
     4201 aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat gctatcataa
     4261 ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat cgttatcttg
     4321 tagcctcaca aggacaaaca caaggaactg cagaggagcc atttttatct caaattaatc
     4381 aaagtattgc aggtcttgaa tcatctatcg caagcctcaa aattcagcaa gctggtatcg
     4441 gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc cgcactcagt
     4501 ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca gaattaaaag
     4561 tacaactaaa tcaagccaca cagcgtttgg aaaacaatac cttaacctcc ccaagtaaag
     4621 gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact ggtacagaaa
     4681 ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact tactacgtat
     4741 cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa ctggagaaga
     4801 ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa actcctacca
     4861 gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct aacgaggata
     4921 gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca aagaaaacat
     4981 attttgatta tttcaaagat aaaattttaa cacattctga ttaattttca gataacactc
     5041 tataactatt tattatctta tcaaaaagga gaatcataac atggataaga aacaaaacct
     5101 aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg gaggattgtg
     5161 ggaagattta ttatataaca ttaatagata tgctcattac atcacataag aacttcatca
     5221 tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct tatttttcat
     5281 cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca ggtggtattt
     5341 agactggtat taggatggct ttccacaatc ttcatgacgg tatagagacc aactcctctc
     5401 tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat gccctcttct
     5461 ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa ggcgattgaa
     5521 acatgaggtt gactaccttc cacactggct tcaatagcat tgtcacaaag gatagacaca
     5581 atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg aacttcgaca
     5641 ttaaagacaa tgttcttatc tctagctttt agaaatttcc ctgctaggag acttttgagg
     5701 gctttatcac gaatattcac caatcgacca aggtcatatt tattgtcctg caatttttca
     5761 ctagaatcct ttaagaccga gtcgtagatc tcttttatct gctccatatc ctcctcttca
     5821 atgcccagac gtaagctagt caagaggttg gtgtagtcat ggcgaaaact ccgtacttcc
     5881 ttataaagct cctctatatg ccgactatac cgctccatct ctctgtagcg caagtcctgc
     5941 tcttggttca gtctctcatg gagtttgtcc ttcaaatagg tatccaattt cttgataatc
     6001 cccataaaaa agagcaggta aaagactagg atgagatggc gaacagtcgt tgattgaatc
     6061 ccctgttcat attcaaagta agacagattt tgtatcacca aatagtaagc ccccattatc
     6121 cagttaatct gagtcaggga cttttgaaaa tctttatcga gaatcccctt tctcaagcta
     6181 gtgaaatcat agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt gaaaattatt
     6241 atacataacc aaataaatga gtagtcatca tatacttgcc cttgtcccaa aaatggaagc
     6301 acaaaatagg aaacacctct ataaaagaga ttcactaata tcattggaaa gagaccataa
     6361 aagagaagga gttttttagg aagttctctt aacaataaga aagataagcc tatgccgtac
     6421 aagggttcca taaaataaga tagataagta tttcctacca tatagccaag cattccaaaa
     6481 acaacggcca acagtatctt aaaaagaaag gccttaaaaa tcctctcaaa agtgagacta
     6541 attccatcta ccttaaagaa tatgacaatt tttaatccgt gagtaacaag tgtatacaac
     6601 aatatccaag caacattcat aaactctcct agctcagtgt aagttattaa tagcctcaga
     6661 cacttccctg accttataac gcgcgattag acaacttcca ccattgggaa agaaaagcag
     6721 tttttctttc ttatctaaat gcaccacatt ggcaggattg atgagaaaag agcgatggca
     6781 ctgcaagaga cgaggctcct gcttgagaac ctcctctaga ctcgctgtaa attccagcct
     6841 gtctgtcttg gtatagagaa taacacgatg gggtcttgtg gacgtttcga gatagtaaac
     6901 ctctttaaaa ggatactgga attgagcaaa ttttgattta aagtaaaagc aatcttccgc
     6961 tagactttta ctgtcttgac cattggcata gaggagggct gtctcgatac gagattcaaa
     7021 ctcctctgcc gaaagggcct tatcaatata gtccagagca gacacttggt agcgaaagga
     7081 caggggcata aactccgagt gagtcgtcac aaagacaatc agggcatacg gatcccgatc
     7141 ccgaatcttt ctagccactt ccagaccctt catctcctca tttcgaatct caatatccaa
     7201 aaagaatagc tgatgggccc ccttctcatg cacctctgcc agcagttggt ctggcttgcc
     7261 aaatacctca aaagagctgg gagtgatatg atgttccttc aagagtttct caatcgtcgt
     7321 ttcaatccta gtctgttggg aaaaatcatc ctctaaaaca aatattctca tcttcttact
     7381 ctccctcttt caaccatttt tgacgaattt ctctatagcg acgtctggca tacttgacag
     7441 cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc gattttaatt
     7501 tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca tattggttct
     7561 caagattact taaattttga agcatattaa aactagcttc ttctgtaaca atctgtacgg
     7621 tatgggcttt agttggatgt gtttggatat agtagatatc gtcgacgttt actttataga
     7681 ctgttttctg cgtctgaata atcatatgtt tcattggatt ctcctcctaa gaaaagaata
     7741 acagaaaagt tctagcccaa gcccacccaa tcctaaacag tctttgaaag ctcctaaaag
     7801 gattattttt ctacaacatc atgatgagct tacaactatt ttatcataaa attcactcag
     7861 cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag aattaaaaat
     7921 gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc agattaaagg
     7981 aggcattccc atgacagata cagaccccat caaaagagct catactttga ttactgactt
     8041 aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct ttcaggagca
     8101 attaaactct attcttggtt ttttagccaa ggctgaaaca gtggataatc gattcttgat
     8161 tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg ctcttgatcc
     8221 agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg accaagtcaa
     8281 gaccaa
//
LOCUS       DAVBCR010000080         7933 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCR010000080 DAVBCR010000000
VERSION     DAVBCR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.080"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(124..1350)
                     /locus_tag="VQ508_001778"
     CDS             complement(124..1350)
                     /locus_tag="VQ508_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2490741.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1422..2087)
                     /locus_tag="VQ508_001779"
     CDS             complement(1422..2087)
                     /locus_tag="VQ508_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490742.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2099..2776)
                     /locus_tag="VQ508_001780"
     CDS             complement(2099..2776)
                     /locus_tag="VQ508_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490743.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2798..3031)
                     /locus_tag="VQ508_001781"
     CDS             complement(2798..3031)
                     /locus_tag="VQ508_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490744.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3317..4180)
                     /locus_tag="VQ508_001782"
     CDS             complement(3317..4180)
                     /locus_tag="VQ508_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2490745.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            complement(4575..5747)
                     /locus_tag="VQ508_001783"
     CDS             complement(4575..5747)
                     /locus_tag="VQ508_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2490746.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            complement(5760..6509)
                     /locus_tag="VQ508_001784"
     CDS             complement(5760..6509)
                     /locus_tag="VQ508_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2490747.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            complement(6521..7933)
                     /locus_tag="VQ508_001785"
                     /pseudo
     CDS             complement(6521..7933)
                     /locus_tag="VQ508_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase"
BASE COUNT         2769 a         1490 c         1171 g         2503 t
ORIGIN      
        1 tttcgtacta ttttagattc agtctactat aacacattca gaaaagagaa aaagtctgtt
       61 gattttgacc atcataaaaa gactggcaat ccagtctcaa acatatatta tagaaattct
      121 ccactaaata ctttcacgaa tattcagaag catgacaaag gcaactagaa gaaatagtaa
      181 taaaacaaag ctaactgcca gagttccaaa gctagtagca atggttccca aagctattgt
      241 aaataagcta ggtaaaacaa ccgtaatggc accgatagaa gattgaactg ctcccattga
      301 ctcctcaggt atttgtttaa agacgagttc ttgcaatcta ggagagagaa tacccgcgaa
      361 aaaggcatcc aaaaagctaa agatcagaat caagccaaaa tgagctgtcg caaagcccac
      421 taggagtagt aattggagta ggagtgaagc atacagggct atttttatgg aaatggtatg
      481 ttgcagatag ccactcatca gacttcccaa aataagggct gataattcta gtgtggctaa
      541 caaggcaaga gattgaccag tttgtaaatt caaaaagggc tggttcctta aaaatagagt
      601 ggaaatagga accgtaacat ttatcactgc ttgactagta gagataataa acaaaaccaa
      661 gagcacctta ttcatattcc atatcaattt cgatgattgg agcaaatgct ggcaaaagga
      721 ttttacagag agtccttctt gatagctaat cgttttttct actttcaaga ggtcagtttt
      781 tatgaagagg atacctaaaa atgcgattaa aaaggtaaga gcgttcagta aggaaataaa
      841 ctggatggat agaatgccta gtaagactcc tcctaggata ttactgattg ttttcactaa
      901 actaacagtt gactgtttaa atccaatagc ttctgccaga tggtcttgcc caataattct
      961 aatgaaaatc ggagtgagca tggcgcctga aaaataactc aatgtgtcag acaagaggtt
     1021 aagcagacaa ataaaggtta ctagcaacaa ggaaaaagac tgccctgaaa gcgataggga
     1081 cactatagag taaagcaaaa attttgtaaa actaatgact gtgtatttca agacacgatg
     1141 atgttgaaaa tccgccaaaa ctcccagaaa gatttgtaga acttggggaa gggtttctga
     1201 aatcgtgata agtaaaatcg ccaaaggggc aaaagacgca tctgccacat aattcaggaa
     1261 ggccagataa aaaatcgtat ccccaagcgt tgaaatccac tggttgatag ttaattgcct
     1321 aaaatctcta ttttgaagaa atactttcat cacaactcct tcttaagttc aaatgggaat
     1381 ctttccccag aaatagaccg cgatactact aacaaccaaa attacagtaa catcaaaagc
     1441 tgaccaatgc cgttgtagac tatatgcagc ccaataggcc aataaatgga ctttgtcatt
     1501 ctaaataaga ctgcaaatat aagtccgcca cccatataga cgaaaaagtc tgtcaagacc
     1561 caaccgtgat tactaatgtg cgagacccca aataaaatag cagaaccaag tacatctagc
     1621 ccccatttct ttcctttttc cagagctgtc atcaccaatc cacgataaat catgtcttca
     1681 aaaatgggac ctgcaatcac aggataaaaa aaatacatca aaaatgccgt agctcctgta
     1741 aaagtaggag cagcatgttg ataagaaatt tcatttcgag taggtgggaa aagaaaaaag
     1801 gtgacaaaat tccaagcaac aaaagcaagc agagctagga aggaatagaa aagataggat
     1861 cctttaaact ttctactatt gattttctgc catttccccg accaaatcat agcaataaga
     1921 gcaaataaaa ccacaagaaa attcaacatc atatccgaca gataataggc aaagtcagat
     1981 agcccagcaa caaggtcgct gcgtaaaact aaaacactga acttctggtc agcaataact
     2041 agtagaaaaa ctataataaa gtagcggtgt gagattatct ttttcatata tcacctttct
     2101 aatatccaaa taccaataaa gtaacaatga gtaagaagct attccatgaa gcatgcagag
     2161 ctatagccca atagatggat cgggtgtagc gaaacatcat acaaaatatc aagcccattc
     2221 caaaatactt tatgaaatct gtcgttatcc aaccatactg caaaacatgc atagcgccaa
     2281 atatggcagc ggaaacaaga acatcaagat agtatctctt aactttagat aaacttgtca
     2341 tcaaaagacc acgacaaaca acctcttctg atacaggtgc gataatacta gtataaagta
     2401 ttcgcgtaac aaaatagcta attcctgtta aactggtggc tacttctacg actgtacttc
     2461 cattctgggt acgaggaaag atataggttg ttagatttgc ccacacgaac aataagaaaa
     2521 aagaaagaag gaaaacaccc aggtaagacc aacgaaactg gaaacgacca cactctttcc
     2581 aatgttcact tttgacaaaa gcaattgtag ctatagttcc cagaataagt accaataaaa
     2641 cttggaacac atagtacata ttatcagaca aagcaaccat aaaatctaag tctgatgtga
     2701 cattaaaaat gaggtaataa gtcaaaatca acaagccagt tgctaggtga aatttcactt
     2761 ctttcatttt cttcatccta ttatctccta taagagccta tcttctacgg cggccaaaca
     2821 atccatctgc taaatctata gtccaatcaa aagctccacg attaggactc atcccttgat
     2881 tgccccaacc agggtaaatt cctgggacgc cccaaccaga tataccactt cttccaccac
     2941 ctcccataga atttacgagg ttgcctcctc taacatcttg caactcagct tctgtcaatt
     3001 ccattgtttc tgcaaattgt aaatttaaca tcttttacac tccttcaatt atcttcattt
     3061 gtaaaccact tctgcgacct aggatttgct tcaaatgttt tacaaggaca gtataacacg
     3121 gaaattggct tattttagaa aatcgcatat ttgatatttt ttcttataga aatttcagat
     3181 ttgcgatttt ggtagatttg attatttccc tggtataata aagttattac taatgaggag
     3241 ggaagtaata tgaagaaaca agttttaaca ttattgacga tcgttgctga tattattatc
     3301 tttttcccat ttctaactaa tcgataagct ctttgtattg ctgcaaacgc aattcaaaaa
     3361 gggctgtcaa ttgtggattt tctaatactt gcagagattg gataaagtgt tcaatctctt
     3421 tttgattgct tcctttggtt tgaagaaaaa tctgcatttt ctttaaaaac tgccacaata
     3481 ctttttcaaa aacatcgtat ggacggagca tgcgctccag ctcagcttca aagattggaa
     3541 tataagaaaa tagcttgcgc tccataagtt ctgagatgat attaagataa ccacttttca
     3601 tataccatcg attgtgtact aactctttaa attccttgga tttttcgagt aaggaggttg
     3661 ataaaaagat cagatcttga ttgctcaaga agggcatagt attgcaaaaa agatagagtt
     3721 caaaccaggt ccaagactca atagcgtaga ggtaggtagt caaaaactcg ctatcctcct
     3781 ctgttagcgg gtagctttta tctaaggaat ggagtgcatt tttaatcaca atggcattca
     3841 aacgacgata ggtctgcgcc atctgttctt gatcgacttc ctccaatagc tgctctaaag
     3901 cagctatatc ctgatgggaa aagcaatcca caacccttct accaattcgc atatgtggag
     3961 attcttgata gttgttgagt ttgtgcccaa actcatcaaa attcatattg atcccttgga
     4021 tggctaggat cagcttgtct gcagacagca tagactgtcc tagttcaaac ttggacaact
     4081 gagaggctgt tagtccagca caagccacat ctgactgctt gagctttctc gccaagcgca
     4141 attccttgta aaattccccc agttccattt tttcaatcat cctaccacct cctagctttt
     4201 gcatattata tcattatttg tgttcatttg tcaaaacatt ctgacacttt ggtgattgaa
     4261 aaagccagcc ttaagctgac tctaaaagga ccgagccttg tacaatacag aactcaatcc
     4321 tcgttctaaa tagaatgtct aacttttttg ggcagtacac gagcttgggg aacagccact
     4381 ggaacgatga ggtgtgagct caaaatatcc tccagttatg tttttcctaa tagtataccg
     4441 gaagagtgaa aggattttat aatggagcgg ttacaaagaa cctactttct attaaacagt
     4501 atactatgaa aatgtgaaaa tttaacattt ttttgtacaa attttataaa ttattgcctt
     4561 tttaatatca atagttaatc tcttatccag atcccccttg tgtaaacttt atctttataa
     4621 gcttcaaggc ccctatccca tctatttgca acaattagat cactttgttt tataaatagt
     4681 tcaaaattct tttcaaaaat tacgttatct atactaacgt ttaaatttgg ttcatatact
     4741 aaaattttta taccgacaat caatagttca ttaattatac ttaaaatagc tgactctttg
     4801 taattatctg aattatattt catccccaat ttatatattc ctactatctt tggctttcgt
     4861 tccaatattt gtttaactat gaactgtttt ctatttgtgt ttgaaatatc aatcgcttct
     4921 atcactgggg catttatttc tataaattct ttttttaatt gtctagtatc tttgggaaga
     4981 caatatcctc caaatccaaa agaaggatta ttataaaaat ttccaattct tggatctaaa
     5041 caaacacctt ttattacaac ttcagcattt aaacttctcc tctcagcaaa agaatctagt
     5101 tcattaaaaa aagcaacacg gagagctaag aatgtgttag aaaaaagctt aattgcttct
     5161 gcttcagtag gagaaactaa cataacattt ttaatattgg cagtactatg agtactaatc
     5221 gaaaggaaca actctgcaat ttttcttcct tcaactgtct catctccaac aactatgcga
     5281 cttggatata aattatcata tatagaacaa ccttctctca aaaattcagg gacaaaaatg
     5341 atattttttg tatcaaacag cctttttaat ttgtttgaaa agccgatcgg aactgttgac
     5401 tttaaaataa tctttccatt aggttttacc ctcagaatct tcgataccgt ttgttcgatt
     5461 tcatatgtat taaaactacc aattttctca tcataatctg tcggaagcgc aataatataa
     5521 taatcaatat tatttttaat ttcagaaaat gtatcaaaaa aagtaatatt taagttattc
     5581 tcgcaaaaaa acttcataag ctcttcattt ttagatggaa gagtgccctt ttttaaatta
     5641 tttattttta cagaatctat atcatatgca acaactttat atttagatgc aaatagtaac
     5701 gcgtaggcca gcccaacatg ccccaaacca attactgcta tattcataaa actacttcct
     5761 tatttcttaa tccaaaatct aatagaataa gctgccccat tccttaaata caactcttta
     5821 atattgttta aaagtttttc aactgatttc cagattatca aaatctgaga tttatagcac
     5881 aatattgatg atattctatc aatataatgt ttttcatcaa gttcctcttg atacattttt
     5941 aattctttag tttttcccat ataactaacc atactactat cacttacata tgggaagtcc
     6001 tcataatata ttactttata acgcataaat tcaagcgccc ttccaatact attcacaaaa
     6061 acatgatcaa catggtcacc aagtgaaagc ggacaatata cgacacattt gtcgtctaaa
     6121 tgcattaaca gctcttttat gatatcattc tttaatgtgt cctcattttt taatttacta
     6181 tagatatgac ggtatagaaa attgccattt ctatctttcc tatagagaca ttcatagtac
     6241 gataagtgtc taaaatcaca ttgtagacgt tcacaagcta acctgtcttc tttcttcctt
     6301 tcttcaatcg gatatttccc aaggttacac aacttatgaa attgcttagc agagggctgt
     6361 agctgttggc tcaaagggta accagaaaat atagtaataa caagtacaat ttctccttct
     6421 gaagttaatt ttgaaatata atcaccacag gaaaaaattg cgtcatctaa atgtggagat
     6481 aaaaagatat acttagtatt gtcactcata accattccct ctacaattta tctaaaaact
     6541 cactaagtgt ctgattaaat tccacatcat caaaaaaatt caccttattc ttaataatga
     6601 atatttcgtt aaataaacat atatataaat atttcaatat cctttcaata tcatcctcta
     6661 aattctcctc aatattttgt atcagcccat ttacaatctt attaaaaaag ataagctctt
     6721 tatctctaaa attaaatatt ttcatacaac tgttgtatcg aaaaatatat aaaataattt
     6781 ttactaatgt ttgaatattt aaacaactaa ataaatgagt tgtacccggg acactattta
     6841 tgttatcaag aacactatct tgaaacctca actcacagtt ctttttgtga aattcttttt
     6901 tatcgtttag atctgatatt tttttagaca tttcaacaat ctcagacatt ttatatggat
     6961 atctaggatg aatgccaaaa ctatgcaaaa gtaggctatt tttctttaaa acaattgaat
     7021 ttgtaacata aaatggatag tcggaataac taaaatatac tgacttctgt ttatcattac
     7081 aaaattgatt gtagttcaca gaatgatact taaaagtcat tatttcatcc gctgactcgc
     7141 cacaaagaat acttgtgttc cctttttgga acaacaacct gcctaaatag tactgcagta
     7201 tgacaccaca ttcaaagaga cttccttcca atctccatac tatatcggga aaatattcta
     7261 aatcttgggc tcattacagt atcaactaat aaatttatac cataaaattt tgcaatttta
     7321 gttacttcag gaatctcatt aattcctttt tctcctccta tgcaaaatgc attaattgaa
     7381 gaattcgtat aattcgctaa tgtatataat atataatttg aatcaaaacc agatgatagt
     7441 gtaatattag cctctcctgc ttcactatac actcttaata tagattcacg tagcatagag
     7501 tcccaattct ttaatacttg ttctttagta aaattgtttg aatcattata atcaatacca
     7561 catatacgaa gagtattatt tacagaaatg taagagaaat attctaattt attaatttcg
     7621 gttactaaag tatttgaatc taaaataaat ccatttctca taaattcttg tatttttttg
     7681 ttatttaagg taacgggaac atgagataac ttcatcactt tctttaaaga tgtactatat
     7741 atagaacaca cctccataaa cagtatagta taaattaaag tatgaagtcg ttatatcttg
     7801 aaaaattttt aattcttttg tcttcgtatc aaaaattata attaaaaaca ttccggtaat
     7861 attctttatt aagctaattc cataaatctt atacaatcca gcgaattcat gcaattccaa
     7921 ctttatattt tta
//
LOCUS       DAVBCR010000081         7561 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCR010000081 DAVBCR010000000
VERSION     DAVBCR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.081"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..463
                     /locus_tag="VQ508_001786"
     CDS             <1..463
                     /locus_tag="VQ508_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2490748.1"
                     /translation="MEPISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTL
                     YEAADMDGANRWHKMWHVTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVS
                     DVLSTYVYRQGILNSDFSYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            474..1403
                     /locus_tag="VQ508_001787"
     CDS             474..1403
                     /locus_tag="VQ508_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2490749.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            1417..2856
                     /locus_tag="VQ508_001788"
     CDS             1417..2856
                     /locus_tag="VQ508_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490750.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            2925..4502
                     /locus_tag="VQ508_001789"
     CDS             2925..4502
                     /locus_tag="VQ508_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2490751.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            4562..6022
                     /locus_tag="VQ508_001790"
     CDS             4562..6022
                     /locus_tag="VQ508_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2490752.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            6044..6499
                     /locus_tag="VQ508_001791"
     CDS             6044..6499
                     /locus_tag="VQ508_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2490753.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            6586..6991
                     /gene="cdd"
                     /locus_tag="VQ508_001792"
                     /pseudo
     CDS             6586..6991
                     /gene="cdd"
                     /locus_tag="VQ508_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            7004..7453
                     /locus_tag="VQ508_001793"
     CDS             7004..7453
                     /locus_tag="VQ508_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2490754.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         2655 a          974 c         1418 g         2514 t
ORIGIN      
        1 aatggagcca ataagtttct ttacaaaatc tgaatggttc ttgcctctct acattggttc
       61 aggagtatgg aaaacaatag gttggggttc tatcatttat atgggagcat taaccaatat
      121 agattcaact ttatatgaag cagcagatat ggacggggct aatagatggc acaaaatgtg
      181 gcatgtaact ctaccaagtt taagacctac agtagttact ttgtttatac taaatgctgg
      241 tggtatcatg caagtaggat ttgaaaaggt attcttgatg tctagtcctg caacatatga
      301 agtatctgat gtattatcta cttatgttta tagacaaggt attttaaatt ctgatttcag
      361 ttactcagct gcagtaggat tatttaataa catagtagct ttgttatttg ttttattagc
      421 taataagatt gctaaaaaac ttggcgaaga aggaattgta taaggggggt aaaatgattt
      481 tttcaaaaaa tacaaaaaag agtaatagag acaaagaaac cttcgttgat tatctcttta
      541 tattcatttt aataataata accttactta tgttgtatcc attatggtgg gtagtgatga
      601 cgtcactttc agaaccagtt tggctatcta aaaacgttgt taaattattt cccttaaaaa
      661 tagatattac tgcatataag tttatttttg gggattctaa actatggcaa gcatatggta
      721 atagtgtatt gtatgctgca ctatctacaa ttgttagtgt tgcggtatgt agtatgtatg
      781 cctatccact tacaattcct gaaattaggg gtaaaaaatt ctggaatact cttattttga
      841 ttccaatgtt ctttagtgga ggaatgatcc cgctttatat acaagttgca aacctcggtt
      901 tattgaatac tgcttgggct cttgttttac ctggtgctgt ttctacatat tatataattt
      961 tgtttagaac acagtttaaa tcaatcccaa tggagcttcg tgagtctgct agaattgatg
     1021 gggcaagtga atttagaatt ttttggaatt tagtattacc attatcaaaa gttattttca
     1081 tgattatagc actttattgc atagttggtt catggaatag ttttactcct ccattacttt
     1141 atttgaatga cgacactaag atgcctttat ctatttattt gagaaattta gtcgttacaa
     1201 atacaactga tactgctgaa ttatcacttt ctgcaggtaa tacatattac caacaaattg
     1261 ctgctcaagg tggaggtatt ggtatgcgta ttgctattaa gatgggtact attgttgtta
     1321 gtgttattcc aattatgatg atttatccat ttattcagaa acattttgtt aagggaatcc
     1381 tgctaggttc tgtgaaaggt taataaggta attattttgc aaaatttaaa aaaatatctt
     1441 ttattagata gcggtttagt agttgggtat acattagaaa atgctcaatt aaaattaaat
     1501 aagctaaaaa aagatactga aaacaatcca ctatttggtg aagagtattt tgccgaaaat
     1561 cctaaattat gggaagttcg ttttgataat tcatatccaa acgttatcta tgataaagaa
     1621 ttgaaattat atagatgttg gtatcaaaca tttgttagtg atgaagcaag cgaagagact
     1681 cctttagcag aacgtggaga gaaggaatac attgtcaagt catctagaat gacagcactt
     1741 tgctatgctg aaagtaaaga tggagtaaaa tgggaaaaac caaatctcaa tctagtaaaa
     1801 tttaaaggtt caaaagataa taatattgtt ctgatgcatg cacacggtgc aggtatcatg
     1861 ctagatgaac aggagttgga tcctaagaaa cgatataaat tagtttcaaa attagaattt
     1921 tctccgaatc ttcattacat ggttgttgtt ttttcagaag acggtattca ttttgacgaa
     1981 cctattccat ggaaaaaatg gaatcctgca gcagatagtc ataattttcc atttagagat
     2041 ccaaaaactg gtaaatacgc tgttattaca agaacctggg caaataacgt acgtctaagt
     2101 gcaattactt tcagtgatga ttttattaat tggtcagaac ctaaagaaat ccttagggga
     2161 gatggatttg aagatcaaat ttattctatg ccagtaattt catacgcgga tatctattta
     2221 ggttttgctt caatgtacca tgaaggcgac actttaaatg aaaactatga taaagtagac
     2281 ttagaattga aattctctgc taatttggaa caatgggata gtgtatgtaa gggcgactat
     2341 ctcattgaaa ggggaggtgg agtatacccg cagaatgctg attttgatgc tagttgtatt
     2401 ttcacggctg ctccaataat tgaagatgat aagatttggt tctactatat gggtggaaat
     2461 ggtaatcaca ctggcttccg agaaagttca ttagctagag gatatattaa gaaagaccaa
     2521 tttgcttatt atactaataa gagagaagga attccagcta aaatatctac agttagcttc
     2581 caaacttacg atgatgaaat ttatatctta gctgatattc ctgaaaatgg ttatattaaa
     2641 gttgcggtag gtactaaata cggtaatgct tattcagatt tcggtcagga agatatctct
     2701 cttgttcaag tttccgatgg taagtataag ttggaattta ataaaaaatc tatcttagat
     2761 ttaggtaatt ctccagttgc gttcaaattt gaaatcgaga atgcaaaatt gtatggtatt
     2821 gaaggtagta ttcagcaaca ctatctcaaa tattaaccag aataattcgt taacaaataa
     2881 tctctatatt agtggagata gatatataaa atggaggtct tattatggta aagcttaata
     2941 aggtattaaa agggagtgtt gcattcctga gtgcaagttt attgctagca gcttgtggca
     3001 ataacgctaa ttcaaagccc gctgaaaaaa cagctgatgg taaaacgaaa atttcgtttc
     3061 tatctacatg gcaaattaat gatgttcgtc cgcctaaaga tgaaacactt gttggtcaat
     3121 ggttagaaga acagctaggt attgatattg atcttgtaac tattcctggc gagggagtaa
     3181 gttctaaagt aaattctctt attactagta accagttacc gactgttttg ttgacaacag
     3241 gagataagag tgatatcgct ggtatcaaca aacttggtaa acagggtgca ttcttagatt
     3301 tgtcacaaca catggacaaa ctaccaaact ataagaagta ccttgaaaaa aataatgcat
     3361 tagctcaaat agaagatgat gaaaaacata tttatgcttt cacaaaattc tttactgatg
     3421 agaatatcat gtatactaca ccaattctga gaaaagactt acttgttggt tcagaatttg
     3481 aagatctttc aaaaattaaa actgttgatg actatacaaa agttttgaaa tatttgactg
     3541 aaaaacaagg ttctccagca tttattcaac gtaatggcta tgaaggcttc atgaagcgag
     3601 ttacaccatt gtggaattta agccatcgta catactatga ctatgaatca gattcatata
     3661 aacatccagt tgaacaacct caattgaaac aattcgttga atggttgaaa gaattacgta
     3721 aagataacgt acttcaccct gactgggcag ttatgaaaga tgaaacttgg gaaggtctat
     3781 tggcttctaa caagggttca ttcactgttg accgtatgaa tatcattgga gataataact
     3841 ttgataaatc atttgattgg cagccacttg tttatccaga aattaatgga aaacgttatt
     3901 tgcaaccgaa aacaccgtac atttctgatt caggttgggt aatcaatgcc aatgcaccaa
     3961 aagaagaaat tgataaagcg ttagaattct ttaatttctt atacgctgat gaaaatcaag
     4021 aaaaacttgc tattggattt gaaggtaaaa catttacacg tgaaaaccct aatactcaag
     4081 caggtattaa atggttgatt caaatttacg gtgaaaatgc tggaaatgat aaagcagaac
     4141 tgctgtttaa acatggtaac caagcattta ctcgcgttca aactaaatta gatattgagg
     4201 cacagccagg aacattccct aaagttatgt atgatcaagt agataaaatt aagaaagatt
     4261 taggtggttt tagaccagat actccggttc tttcctttac aactgaagaa ttagatgttt
     4321 taactaaatc tgaagcagga ttaaatactt tctttgatga aaatattatt aaattcattg
     4381 aaggacaaag agatatgagt gagtgggatt catttatcaa tgaagctaaa tctaaagact
     4441 tggataaagt tacaaaagta tataatgatg cctacaaacg tgtcaaagat agaatgaaat
     4501 agactaaatg gtagctctct ggtttttcag agagctacta ctgcctgagg gggagtaagc
     4561 tttgtatgat gtagtaatta taggtgcagg aagttcgggc gcattggctg caattgcttc
     4621 ggggcgtcta gggttaaaaa cttgtctaat agagaaggga agtagaattg gtggagttcc
     4681 tattaataca ctaatggggt catttgctaa tctatatttt gatgattctg gtagagtaaa
     4741 tgcaagtaaa attgttgggg aattaataga acgtattatc aaaaaaggtg gtactgtctt
     4801 taactctatg gaagaattga tgaatgtaaa tgatttttat cagattacaa ttccctatca
     4861 accagaagtt tatgaatcgg ttttaactga tatgttgatt gagtctaaag ttgatatttt
     4921 actaaatgtt gaagttaata atattattta tgatagtaaa aatattaaaa gtcttgaaat
     4981 tttaagtaat caggaagtta aattgattga ttctaaagtt tttattgatg ctacagggaa
     5041 tgcaacaatt gcttctgcta gtggagctga tgtttatgaa aaaaattctt cttacggttg
     5101 tttaatgaga gttggaggag ttgatatctc aaagactata gaacatattg aaaaaacaaa
     5161 gcagtggaac tatatagaag gatataatca gtggttagaa gaagtattag caaatcgaaa
     5221 gtacagagcc actggagcag ttttaaaaga tccagtttct tatgatcatg cccctatgct
     5281 ttcaaaagac gaccatttta tgaatgatga aaaatggaat tacattaaag aaagatggga
     5341 aaaatttaga tttatttaca ctttagaagt atcactattt aggaatgaat taaaaaaagc
     5401 tgtagaatct ggggatttta attttgaaat ttatcatgat gataaatctg gagtcactat
     5461 gaatggcgac ggtataagct atggttcatg gggtcaaaat gtagcattaa ttaatgttgc
     5521 aaaagcattt ggattcaatc ccaaatatat ggaagatgaa tcacatgcta atattctttc
     5581 tagaaaatat aatttaacat ttttatcttt tcttaaaaaa tatgtaccag gttttgaaaa
     5641 ttcttttatc cttgatcagg gaactagagc tactaacaga tcatgtaggc acattgataa
     5701 ttcatctatg atggacgaaa ttcttgattt tccaatgtat acatttaaga ctatgtatag
     5761 ctatcaaact cctaaagaaa taacgtacaa atcaattgta ggtggaaaat tgaataattt
     5821 gtttgtaata ggtaaaggtg cttaccaatc cgaaagtttt agatcacaaa ttagttgtat
     5881 gttaatgggg atatcatcag cagcagcggc aaaaactatt attgaagata aaagcaatac
     5941 tctaaacatt aatagagatt tgtttaaatc gaacttagat atattattta catgggagtt
     6001 ttctaattgg attactgatt aacaaataat aagggaatat taaatgaatg atactaataa
     6061 aaatcaatac tttgtttatg aatttgatac tagctttgta gaaatgtctt attctcagtt
     6121 ttttgaagga tattgtattc ttttttcaaa agaaccctat ccttctttat ctgatatgcc
     6181 aatggaattt agaaaagaat ttttaatgga aatgagtata ttaggtgatg cgatgcttga
     6241 agtgttaaat gcctatagaa ttaattatga tatcttagga aattcaacac ctgtacttca
     6301 tgctcattta tttccgagat acatgtggga agaggaagag agaagaaaaa ctgtagttca
     6361 tagatatgat tcttcagttt ttagcaatcc tgagtttgaa tttactatag aaaagcatgg
     6421 accaattatt aaaaaactga ggtctgctat tgatagacat tatagagagt atcaacaatt
     6481 attagatgat tcaaactgaa ctccacattt tcgattagat ttaatacctt gtatattaca
     6541 actaggttgt ggatgatata attttttgaa aaatatattt taggaatgac ttataaagca
     6601 attatatcag aggctattaa atcaatggcc aaagcttatg ctccttattc tgattttaag
     6661 gttggagctg cgttagtaac tgaagataaa aaattttttc tggatgtaat atagaaaatg
     6721 ctgctttttc tcctacttgc tgtgcagaac gagtatcttt ttataaagct gtatttaaag
     6781 gagaacgttc gttttcaaga acatgtattg taggtggaaa aaatggtatc ttatcatctt
     6841 ttatagcacc ttgcggagtg tgtcgccagg taatgataga atttgtaatc ctgattcatt
     6901 tgaagttata ttggctagtg atgttgatac ttataaagta tataaactta aagaactact
     6961 accagttagt tttaatcaag aaaatttata acaggaggga actatggaat tagttgaatt
     7021 taaaaatgga catgttaata cggttcatga gcatatcgca ataacgaaag taaaagagtt
     7081 tctaaataat catgatatat taaaagataa aggtggcata cataaaattt ctactgattt
     7141 cttttacaat ataattgata tggaaactac aactgaagag aatagaccgt gggaaagtca
     7201 taaggaatat tatgatgtcc attatctgtt aaatggagaa gaaacaattt tatataactt
     7261 cttgtctcaa atggaattgt ctgaatataa agtagatgat gattggcaac aaatgaatgg
     7321 gacagcttta tttagtatta aattaaagaa agatatgtta cttttgttgg aaccaaacga
     7381 tgcacataga acaggcctat tggttgaaga accacttaat attaagaaag ttgtttttaa
     7441 ggtaaaaatt taactgaaag tactttcggg gattaagaat tttaaaatat ttaagaaatt
     7501 gtattctaaa gttctaaaat tatagatatt taatttaaag cttgttaaaa tagaatagta
     7561 a
//
LOCUS       DAVBCR010000082         7549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCR010000082 DAVBCR010000000
VERSION     DAVBCR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.082"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            172..1563
                     /locus_tag="VQ508_001794"
     CDS             172..1563
                     /locus_tag="VQ508_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2490755.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            1677..2519
                     /locus_tag="VQ508_001795"
     CDS             1677..2519
                     /locus_tag="VQ508_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2490756.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            2792..3553
                     /locus_tag="VQ508_001796"
     CDS             2792..3553
                     /locus_tag="VQ508_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2490757.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ508_001797"
     CDS             3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ508_001797"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2490758.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5100..7136
                     /locus_tag="VQ508_001798"
     CDS             5100..7136
                     /locus_tag="VQ508_001798"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2490759.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7178..7435
                     /locus_tag="VQ508_001799"
     CDS             7178..7435
                     /locus_tag="VQ508_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2490760.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2364 a         1325 c         1688 g         2172 t
ORIGIN      
        1 aggtctacta ttctagcttc aatctactag aaattccata gatagaaaac tacataatct
       61 ctacagatac ggatgttgga gttgatgtaa gatgctttgg cttgctagag gaattgtgga
      121 ttgccaaatt gtatcattga aattattgct caaatttgtt atgatataaa tatgaataaa
      181 agtagactag gacgtggcag acacgggaaa acgagacatg tattattggc tttgattggt
      241 attttagcaa tttctatttg cctattaggc ggatttattg cttttaagat ctaccagcaa
      301 aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga aagatgatca attgagtgag
      361 ggaaatcaga aggagcattt tcgtcagggg caagccgaag tgattgccta ttatcctctc
      421 caaggggaga aagtgatttc ctctgttagg gagctgataa atcaagatgt taaggacaag
      481 ctagaaagta aggacaatct tgttttctac tatacagagc aagaagagtc aggtttaaag
      541 ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt tagttgcttt taagattgaa
      601 gagactgaaa agaccagtct aggaaaggtt cacttaacag aagatgggca accttttaca
      661 cttgaccaac tgttttcaga tgctagtaag gctaaggaac agctgataaa agagttgacc
      721 tccttcatag aggataaaaa aatagatcaa gaccagagtg agcagattgt aaaaaacttc
      781 tctgaccaag acttgtctgc atggaatttt gattacaagg atagtcagat tatcctttat
      841 ccaagtcctg tggttgaaaa tttagaagag atagccttgc cagtatctgc tttctttgat
      901 gttatccaat cttcgtactt actcgaaaaa gatgcggcct tgtaccaatc ttactttgat
      961 aagaaacatc aaaaagttgt cgctctaacc tttgatgatg gtccaaatcc agcaacgacc
     1021 ccgcaggtat tagagaccct agctaaatat gatattaaag cgactttctt tgtgcttggg
     1081 aaaaatgttt ctgggaatga ggacttggtg aagaggataa aatctgaagg tcatgttgtt
     1141 ggaaaccata gttggagcca tccgattctc tcgcaactct ctcttgatga agctaaaaag
     1201 cagattactg atactgagga tgtgctaact aaagtgctgg gttctagttc taaactcatg
     1261 cgtccacctt atggtgctat tacagatgat attcgcaata gcttggattt gagctttatc
     1321 atgtgggatg tggatagtct ggactggaag agtaaaaatg aagcatctat tttgacagaa
     1381 attcagcatc aagtagctaa cggctctatc gttttgatgc atgatattca cagtccgaca
     1441 gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc aaggttatac ctttgtgacc
     1501 ataccagaga tgctcaatac tcgcctaaaa gctcatgagc tgtactatag tcgtgatgaa
     1561 taagcaagaa aaaataggtc tgttagatat ttgacagact tattttttac agaatatagt
     1621 actacttaaa aaatgtttta tgctataatt gatgaataaa atagaaggag aagcatatga
     1681 atacctatca attaaataat ggagtagaaa ttccagtatt gggatttgga acttttaagg
     1741 ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc cttgaaggct ggttatcgtc
     1801 atattgatac ggcggcgatt tatcagaatg aagaaagtgt tggtcaagca atcaaagata
     1861 gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct ttggaatagt cagcaaacct
     1921 atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa actgggcttg gattatttgg
     1981 atttgtattt gattcattgg ccgaacccaa aaccgctcag agaaaatgac gcatggaaaa
     2041 ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta tcaagaaggg aaaatccgtg
     2101 ctatcggcgt tagcaatttt cttccccatc atttggatgc cttgcttgaa actgcaacta
     2161 tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt gtatcaagaa caagtcgtag
     2221 cttactgtcg tgaaaaggga attttattgg aagcttgggg gccttttggt cagggagaac
     2281 tatttgatag caagcaagtg caagaaatcg cagcaaatca cggaaaatcg attgctcaga
     2341 tagccttggc ctggagcttg gcagaaggat ttttaccact tccaaaatct gtcacaacct
     2401 ctcgtattca agctaatctt gattgctttg gaattgaact gagtcatgag gagagagaaa
     2461 ccttaaaaac gattgctgtt caatcgggtg ctccacgagt tgatgatgtg gatttctaga
     2521 aaatcataaa aagaattgta cattattcta atttttgata taatagtcag caggaaagaa
     2581 agtcttatgg cgttcttcaa gcgagcttgg gatagtggga gccaagtagg gcaaaataat
     2641 gagctggcgc tttctgtagt attttcaaaa acaatgaagt aataaattag ggtggaaccg
     2701 cgtttctgac gcccctaggt taaatcaacc taggattgtc ggacgcggtt cttttgctta
     2761 ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac ctttatcttg taaaagacga
     2821 tagtcaacta gctacatttc gtgattttgt agtaagaaat actgaaaagt tgaaagatta
     2881 tcaatctttt ttaaagaatg aacttgcagt ctgtgattta ccgcaagccg ttatttggtc
     2941 agattttaat gctgctacac agattattag ggaaagcgct gttccagcct atacaaataa
     3001 tagacgaatg gttatggcgc ctgatttagc tgtttggaaa gagttgtatt tgtatcaatt
     3061 gatggactac gagtgttctc agcaaactca agcaatagaa agtcactatc attctttatc
     3121 tgaaaatttc ctcttacaga ttgtaggaca tgagttagct cattggtcgg agcatttttt
     3181 agatgatttt gatggttatg actcttatat ctggttcgaa gaggggatgg ttgaatatat
     3241 tagtcgcaag tatttcttga cagaagagga atttcaagcg gaaaaaattt gtaatcaatc
     3301 tctcgtagaa ctttttcaga agaagtatgg ttggcattca ttgaatgatt ttggttcttc
     3361 gacttatgat aagaactatg caagtatttt ttatgaatac tggcgcagct ttttgacagt
     3421 agataagttg gtagaaaatt taggtagtgt acaagcggtc ttagattctt atcatttatg
     3481 ggcaaataca gaaaaaactt ttcccttgtt agattggttt gttcagcaga aattaattga
     3541 aaaagaaata taaaaactaa aggagtaaac aatgtctaag aaattgacat ttcactgcgt
     3601 cagtggcaga aacctcctta cagtcggact gccccacgct cagcactaga gtgcctgagc
     3661 tagacgcagt actaactcgt cttgcctcgt atgatcgacg aggcagactc atgtcgcaag
     3721 taattatttt ttattaagga gtaaacaatg tctaagaaat taacatttca ctgcatcagt
     3781 ggcagaaacc tccttacagt cggactgccc cacgctcagc accctagtgc tgagctagac
     3841 gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca gactcgtgtc gcaagaaatc
     3901 attttttatt aaggagtaaa caatgtctaa gaaattaaca tttcaagaaa ttattttgac
     3961 tttgcaacaa ttttggaatg accaaggttg tatgcttatg caggcttatg acaatgaaaa
     4021 aggtgcgggg acaatgagtc cttacacttt ccttcgtgct atcggacctg agccatggaa
     4081 tgcagcttat gtagagccat cacgtcgtcc tgctgacggt cgttatgggg aaaaccctaa
     4141 ccgtctctac caacaccacc aattccaggt ggtcatgaag ccttctccat caaatatcca
     4201 agaactttac cttgagtctt tggaaaaatt gggaatcaat cctttggagc acgatattcg
     4261 ttttgttgag gacaactggg aaaacccatc aactggttca gctggtcttg gttgggaagt
     4321 ttggcttgac ggaatggaaa tcactcagtt cacttatttc caacaagtcg gtggattggc
     4381 aactggccct gtgactgcgg aagttaccta tggtttggag cgcttggctt cttacattca
     4441 agaagtagac tctgtctatg atatcgagtg ggctgatggt gtaaaatacg gagaaatctt
     4501 tatccagcct gagtatgagc actcaaaata ttcatttgaa atttcggacc aagaaatgtt
     4561 gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt gcattagaag aaggcttggt
     4621 acaccctgcc tatgactatg ttctcaaatg ttcacatacc tttaatctgc ttgacgcgcg
     4681 tggtgccgta tctgtaacag agcgtgcagg ctatatcgct cgtatccgta acttggcccg
     4741 tgttgtagcc aaaacctttg ttgcagaacg caaacgccta ggttacccac ttttggatga
     4801 agaaacacga gttaaacttt tagcagaaga cgcagaatag tgagtaatac tgtactctaa
     4861 aatcgttaga ggcaagtcga agacttgcta gagggatatg caccgccgta ctgttatgtg
     4921 gggatttttg aaaccttagg ttcaaaaatc agtcagctaa atccactttg atgagattgt
     4981 tggaaatctt gataaattta gcattggatt tccatacaga ctcacagagt tagttagcga
     5041 cgtccccagt acctaaggtg catatcaaaa aagattgaag aaaatgtaaa ggaaaaaaca
     5101 tgacaaaaaa cttattagta gaactcggtc ttgaagaatt accagcctat gttgtcacac
     5161 caagtgaaaa acaactaggc gaaaaaatgg cagccttcct caaggaaaac cgcctgtctt
     5221 ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc tgttcgtgtg actggtttat
     5281 cagacaaaca gtctgatttg acagaagatt tcaagggacc agcaaagaaa attgccttgg
     5341 atagtgatgg aaactttacc aaagcagctc aaggatttgt ccgtggaaaa ggcttgactg
     5401 ttgaagatat cgaattccgt gaaatcaagg gtgaagaata tgtctatgtc actaaggaag
     5461 aagttggtca agcagttgaa gccattgttc ctggtgttgt agatgtcttg aagtcattga
     5521 ctttccctgt cagcatgcac tgggctggaa atagctttga atacattcgt cctgtccaca
     5581 ctttaactgt tctcttggat gagcaagagt ttgacttgga tttccttgat atcaagggaa
     5641 gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac caaaattcag tcagcattga
     5701 gctatgaaga agaccttcgt aagcagtttg taatcgcaga tccatgtgaa cgtgagcaaa
     5761 tgattgttga ccaaatcaag gaaattgagg caaaacatgg tgtacgtatc gaaattgatg
     5821 cggatttgct gaatgaagtc ttgaatttgg ttgaataccc aactgccttc atgggaagtt
     5881 ttgatgctaa ataccttgaa gttccagaag aagtcttggt gacttctatg aaggaacacc
     5941 agcgttactt tgttgttcgt gatcaagatg gaaaactctt gccaaacttc atttctgttc
     6001 gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg aaatgaaaaa gtcttggtag
     6061 cccgcttgga agacggagaa ttcttctggc gtgaagacca aaaattggtg atttcagatc
     6121 ttgttgaaaa attaaacaat gtcaccttcc atgagaagat tggttccctt cgtgaacaca
     6181 tgattcgtac gggtcaaatc actgtacttt tggcagaaaa agctggtttg tcagtggatg
     6241 aaacagttga ccttgctcgt gcagcagcca tttacaagtt tgacttattg acaggtatgg
     6301 ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa atacaccctt cttgctggtg
     6361 aaactccagc ggtggcagct gctattcgtg aacactacat gcctacatca gctgaaggag
     6421 aacttccaga gagcaaggtc ggcgcagttc tagccattgc agacaaattg gatacgattt
     6481 tgagtttctt ctcagtagga ttgattccat caggttctaa tgacccttat gcccttcgtc
     6541 gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg ttggcacatt gctatggatg
     6601 agctgattga tagcctttat gcattgaaat ttgacagttt gacttatgaa aataaagcag
     6661 aggttatgga ctttatcaag gctcgtgttg ataagatgat gggctctact ccaaaagata
     6721 tcaaggaagc agttcttgca ggttcaaact ttgttgtggc agatatgttg gaagcagcaa
     6781 gtgctctcgt agaagtaagc aaggaagaag attttaaacc atctgttgaa tcactttctc
     6841 gtgcctttaa cctggctgag aaggcagaag gggttgctac agttgattca gaactatttg
     6901 agaatgacca agaaaaagct ttggcagaag cagtagaaac actcgtttta tcaggacctg
     6961 caagtcagca attgaaacaa ctctttgcgc ttagcccagt cattgatgct ttctttgaaa
     7021 atactatggt aatggctgaa gatcaggctg tccgtcaaaa tcgtttggca atcttgtcac
     7081 aactaaccaa gaaagcagct aagtttgctt gttttaacca aattaacact aaataaaatt
     7141 tgataaacgg actttatctt attacaaagg agaagaaatg gatccgaaaa aaattgctcg
     7201 tatcaatgag cttgctaaaa agaaaaaaac agaaggctta acaccagaag aaaaagtgga
     7261 acaagccaaa ctacgtgagg agtacatcga aggttatcgc cgcgctgttc gtcaccacat
     7321 tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt acaccagaaa aactacgcca
     7381 agtacaacgt gaaaaaggat tacatggccg tagtcttgat gacccaaatt cataatatgt
     7441 actctttctt ttaacaaaga tagatgaaac gataacaaag agactagcag tttgtgtttg
     7501 ctagtctttt ttcgctaaaa aaggaaccat tatggttcct aaaaactat
//
LOCUS       DAVBCR010000083         7342 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCR010000083 DAVBCR010000000
VERSION     DAVBCR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.083"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(23..857)
                     /locus_tag="VQ508_001800"
                     /pseudo
     CDS             complement(23..857)
                     /locus_tag="VQ508_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(871..1737)
                     /locus_tag="VQ508_001801"
     CDS             complement(871..1737)
                     /locus_tag="VQ508_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2490761.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1751..3010)
                     /locus_tag="VQ508_001802"
     CDS             complement(1751..3010)
                     /locus_tag="VQ508_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2490762.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3102..5264)
                     /locus_tag="VQ508_001803"
     CDS             complement(3102..5264)
                     /locus_tag="VQ508_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2490763.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5372..6232
                     /locus_tag="VQ508_001804"
     CDS             5372..6232
                     /locus_tag="VQ508_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2490764.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6229..7164
                     /locus_tag="VQ508_001805"
     CDS             6229..7164
                     /locus_tag="VQ508_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2490765.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         2329 a         1528 c         1326 g         2159 t
ORIGIN      
        1 gctctttgat tttcattgag tattacttca ctgccccgtt gcttattcct gcgatgatat
       61 ggcgttggaa gaagagatag acaatggtga tactgataat gccgaccacg taagaggcaa
      121 agcttggtcc gtagtcgttg aaatattggc ctgcgtagtt gtattggaac aaaggcagag
      181 tccacatttt ggaatcccgg ttcaagacaa ggagtggcaa catgaagtca ttccagaacc
      241 aaagggcatt gatgatcatg gttgtcgcat gcatcggttt catcattggg aagatgatgc
      301 ggaaataggt tgtaaattga ttagccccat cgatctctgc tgcttcatcc agactttctg
      361 gaatcgagat tttgatatag ccaacataga gaaagagggt ctgtggaatc gcataggtca
      421 agtagagcaa gatcaaacca aaggtattag ccaaaccgag tttactcatc ataaccgtaa
      481 tcggaatcat gatgacttgg aaaggtacga agattccgag gattaagagg gtatacatga
      541 tggtaaaggc ttttctttta ctcatattgc gagcgatgga gtaggctgcc atagggataa
      601 agatcattac tgcaagtaaa gacaagacag tgatgacgac agagttccaa taatagcctc
      661 caatccccat cagctaagag acggctaaag ttgtcccatg tgaagttggt tggaaagcca
      721 aagaaattat ctacaatatc cttagtggat ttgaaggaac taaagagggt agcaaggagc
      781 ggcactaaaa tcagaaccga tcctagaatc aatagaatgt atttgccaat cagggctttt
      841 ctttcatctt gtttcatcat gcttctcctc ttaaatttca aatttcttag atactctcaa
      901 ttggatgatc gaaatcacta caattaagaa gaacaagatt acggcaatgg cattggcata
      961 accgaattgg ttgtttttaa aggcatagtt ataaaccaag agcccaagtg aggttgtggc
     1021 attgtttgga ccaccaccgg tcatggcaaa gacttggtca aaggcagtca gcccaccttt
     1081 tagggctagg ataaagacta tagagacact tggtagcaag taaggcaatt caatgttcca
     1141 gaaaacttgc ttgctagtcg caccatcaat ccttgctgcc tctgtaatct cagttggaat
     1201 agattgcaaa ccagctagga agatgatgat gggcatagcc accccttgcc aaagaaggac
     1261 aaagacagcc gcaaagattg ctccccactt agtccctaaa agactggttt ggagaaattc
     1321 aatatgaagg gcattcccaa tcgctggaag accgtagttg aagacttgct tgaagatcaa
     1381 agccactgtc aaaccagata aaacagctgg gaagaagaac caagcacgga agaaggtttg
     1441 gcctttgatt ttagagttca agacacgcgc aatgaagatc ccgagtgcaa tctcaccaac
     1501 caccatggca atcgtaatga ttgcggtaaa gccaatcgca ttcatgaatt ttggatccat
     1561 gaagaggagc ttaaagttgt ttaagccgac aaatttgtag ttgtaagtca atcctgtcca
     1621 gttggtaaaa ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa ttgcttgtaa
     1681 caagaggggg atgaccacaa aagcccatgc ccaatatttt tgtaatactt ttttcatagt
     1741 ctctctactc ctaatccaca tccgctttca tcgggttaaa gaaggcattc aaatcattga
     1801 ccatgccttg tttatcaccg gtcaagacat agttcatggt caaggtatgg aagtctgctt
     1861 cactggtcca gtattgttgc aaccagacca agtgacgatc cgtaaaggca tattcggtca
     1921 taccagcaag cggtgaatct tctcctgctt gtttgacccc ttcgatcgct gttggagatc
     1981 cgtccacatc gtagtatttt tgcatgactt ctgaacgggt catatattcc acaaaggcat
     2041 tggcttcttt tggatgtttg gtggtggctg agatagacca tgccaagtct cccgcaccaa
     2101 cggttaagct ttgtcctttt tcttttcctg gaatcatgaa ggttccaatc ttaaagttcg
     2161 gtttttgttc attaatcgct gtgatcgccc aagacccatt tggtgtcatg aggacatccc
     2221 cacgtgcgaa ggctccgata acatcggtat agccagcacc ttcccagttc ttttgcttag
     2281 agccattaat gcgaaggatg tccatgacct tgatatcatc tttcataatc ggatccgaca
     2341 atttaatagc atttggttga gaataacgaa ggtattgatt tgcttctttt cctccacctg
     2401 ttgctgtcgc aaaggctaat tgattgtaac cattgagtgt ccaagcatct gcacctgcaa
     2461 ttccaaatgg tgtttgtcct ttagcaacga tatctttgac taactgttca aattcatccc
     2521 aggtttcagg aaccttcaag cccagttctt cgaatttatc tttgttgtag taaattccat
     2581 aagcattagc tgtaaaagga acgttgtaaa ctttttcgtt tacagcatat ttttcagcgt
     2641 agccattttt cacgcgtttc aggtagtctt tgttactcaa atcttcaaag acacctgctt
     2701 ttgcccattc ttgcagttcg atggactgtg ggtaaatatt gaccacatca ggcacatctc
     2761 ctgcgagaac gcgtgtcttc aatacttcac cagcatttgg tacattgacg actttgacct
     2821 ttatcttagg gttttccttc tcaaaatcac gagtgatttc ttccaaggtt ttggtcattt
     2881 cttttttctg gttgaaatac tcgatggtca ctgtgtcatc cgcagattta ccatagttgg
     2941 agcaagcacc gagcccaaac aaagctaaac ctgtagttgc aagaagtccg atttttttat
     3001 accattccat tagaaagcct cctttataaa tttatacacc cttatcaaac tgcacttcaa
     3061 aagttagata gaataaatct aacttttggg gtcagtacat atcatagttt tctaaaaata
     3121 tactgtctac tcaaaaaatc tccttgggat aagataacag ttaagcccgc atacattagt
     3181 tctgcacctg agtaaacttc gccattttcc tgtaatttat atagtccctc ttcatccaaa
     3241 tcttttaatt ttaaagttgt ttccatggtc tctacaacag ataaaacgcg aacgtaggtt
     3301 acaatcgttt gatttccgta attaaattgt acagctgctt cattggatgc agtatcagga
     3361 ttaattagtc tatactgctg tcctaactga actactggtc gtaattcttt atacaagttc
     3421 acctgattag caatcgtagc tttctcttca tctgataaat ttgtcaaatc aagctcatag
     3481 cccaaatttc ccatcattgc tacatggcca cgtgtttcta atggtgtcat tcgtcccatc
     3541 tgatgattcg gtactgctga cacatgagcc cccatagaaa tggttggata gaaataggat
     3601 gaaccgtatt gaattggtaa acgtgcaatg gcatcagtat tatcactagc ccagacttgt
     3661 gggaaatagc gtatcatacc aagatcattt cgtccaccac caccagagca ggactcaaag
     3721 agaatatggc tgtgcttctc tgtcagataa gaaacgagtt cataaagccc cagcatgtac
     3781 tgatgagatt gcatctgtgt ctctagataa gttaatccat tccctagctt agtgatattg
     3841 cggttcatat cccatttaat gtaatcaatc tcatgataag ataggagttg atctaagaca
     3901 cttttcaagt attctactac ctgaggattg gcaagattaa gtactaattg attccgagaa
     3961 taagtatgct catagtcagg aacctgaata gcccagtcag ggtgttgacg atacaaatca
     4021 ctatctacag aaatcatttc gggttctaac caaagtccaa actgcaaacc tctttcatgg
     4081 atagctgaaa tcagactttc tagacttcca cccagttttt cctcattaac aacccaatca
     4141 cctaaagcac gattatcatc aaaacgattg ccaaaccaac catcatctaa tacaaaaagt
     4201 tcaatgccaa ctttcttagc ttcatctgct aactctaaca gtttttctct ctgaaagtca
     4261 aagtaagtag cttcccagtt attgattaga attggacgtt cttttttaga aaattcactt
     4321 agaataatgt gcttcagtac aaaattctga ctttcatgac taataccagt taatccctga
     4381 tctgaatgag tcactaaagc taccggtgtt tcaaagtatt cctcaggggc taacttccaa
     4441 gaaaagtttt ctggattaat gccaatagcc acccgaactt cattcaattg atttttttga
     4501 acaaaagctt caaagttgcc actatacatt agttgaatag caaacacatt cccagcatcc
     4561 tctgtgactc cttgttcgca tagtagaaga gctggtgttt gagcatgacc agaagcacct
     4621 cggtttgaac taatcgaaaa gattccttgt tctacctgtt gacgtctaac agtcttttca
     4681 cgagcataag caccctgcag agttgctatt tcgtaatctg cagctggaaa atcagccata
     4741 aaagaaaaat ctttatggat gacaacttcc tgattactat tattatctaa tttactgtag
     4801 ctagtaatag tcgcatcatt attaaaagta gtataataca aagtcagact aagttgagcc
     4861 ttagaatctt ctaacattaa gacaagagtc tctgtatcgt ccatgctatg tggagaaggt
     4921 aagccctgtg gaccattctg accttttaaa atctttgctt ctacaaatcg aaagtctgtt
     4981 acttcagtta cactatgctg aacctgtata gttggtttcc taaaatctcc taagccatgt
     5041 tgtccaaaaa tctgtcgctg agtatctaaa ctaaaggttc gattagtagc cgttggattt
     5101 cctgaaaagg catggtctcg ttcataaaca ctattggaac ctttatagtt cttaatagtc
     5161 tttcctaaat gtttcaaaag taagtagcca tttcgatttt caataatcaa acttagattt
     5221 ttactctcaa cataaaatag attattctct atcctaactc ccatttactt cacctcatca
     5281 ctttattgat tatattttat cacctgaaat cgctttccga aatagaaaaa tgtctcaaga
     5341 atatggtaaa atgttaggta ggaggtagca catgttagtt ttttcagaat accagactgg
     5401 aacaatcgac cttgccctaa gcttttatgg atatgaggaa tgcacaccta attactcttt
     5461 tggtccagcc attcgtgata catacgttct acattacatt actaaaggac aaggaaaatt
     5521 ttattacaag ggtaaaattg ttgatttaaa agaaggagat ttctttctat taaaaccaga
     5581 ggaactaacc ttttatcaag cagatagtaa agaaccttgg gcctactact ggttaggaat
     5641 cactggaggg aaatcccctg attattttgc tctttctcaa atttctgatc aatcctatct
     5701 catccaatct gaaacttgtc atacccagac tactgcaaaa ctcatctcag acattgtccg
     5761 cttcgctcag attacaaaat caagtgaatt agctcaactc catatcatgg gacaacttca
     5821 tgaactgatg tttcatctgg gaactattgc tcccaatcag aaaaaaaaga atatttcatc
     5881 aacccaccaa ctctatcttg aatgcaaacg attaattgat agccactatc ctcaatcact
     5941 tacaattcaa gatttagcaa aagaactatc cgttcacaga agctacttat caagcgtatt
     6001 caaagaattt aataccttat cacccaaaga atacctactc tacgttcgaa tgcaccgagc
     6061 tagacaactt ctcgaaaata cccaagagtc catcaaggta attgcatact cggtaggttt
     6121 ttcagatcca ctccattttt cgaaagctta taaacaatac tttaatcaga ctccaagtca
     6181 tacaagaaaa gaatactctc aataccaact agtgagaaag gcaacattat gaaatcctac
     6241 caagctgtct accaaatcct atctaaagaa accgactata tcagcggaga aaaaatcgca
     6301 gaaaaactat ccctaagccg aacagcaatt tggaaagcca tcaagcgact agaacaagaa
     6361 ggcattgaaa ttgatagtat caaaaataga ggatataaac tgatgaatgg tgaccttatt
     6421 cttccagaga ttctagaaga aaatcttcca attaaagtca gctttaaacc cgaaacaaaa
     6481 tcaacacaac tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa taccctctat
     6541 ctagcttcct atcaaacagc aggccgaggc cgttttcaac gttccttcta ctcaccacaa
     6601 ggtggtattt atatgacact ccatcttaaa ccaaatctcc cctatgacaa attaccatcc
     6661 tacacactac ttgtagctgg agctgtctac aaagccatta agaacctaac tttaatagat
     6721 gtcgacataa aatgggtcaa tgatatctat ctaaacaatc ataaaattgg aggaatcctt
     6781 actgaagcaa tgacctctgt agaaactggc ttagtcacag atatcattat tggagtaggt
     6841 atcaatttca ctattaaaga cttccctcag gaattaaaag aaaaagctgc cagcttattt
     6901 aaagctacag ctcctataac aaggaatgaa ttgatcatag aaatctggcg tgctttcttc
     6961 gaaacaccag cagaagagct attataccta tacaaaaaac agtcattcat tctaggaaaa
     7021 gaagtcactt tcacactaga gcaaaaagac tacaagggac ttgctaaaga catctcagaa
     7081 aatggaaaac ttttagttca atgtgataac ggaaaagaaa tctggctaaa tagtggcgaa
     7141 atttctctca atagttggaa gtaaaataac acaattataa tataaacgat ataaaaataa
     7201 cttcagatta gtaattcaat taagttttac ggatctgaag ttttattgtc tctaaaaaca
     7261 aaaaagagag ttacaaactc tcattaaaac gggctctata atatttgtag tgggtaaatt
     7321 ccctatggat attatggagc ct
//
LOCUS       DAVBCR010000084         7324 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCR010000084 DAVBCR010000000
VERSION     DAVBCR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.084"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            66..935
                     /locus_tag="VQ508_001806"
     CDS             66..935
                     /locus_tag="VQ508_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2490766.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            939..1895
                     /locus_tag="VQ508_001807"
     CDS             939..1895
                     /locus_tag="VQ508_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2490767.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1895..2659
                     /locus_tag="VQ508_001808"
     CDS             1895..2659
                     /locus_tag="VQ508_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490768.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2659..3369
                     /locus_tag="VQ508_001809"
     CDS             2659..3369
                     /locus_tag="VQ508_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490769.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3677..4333
                     /locus_tag="VQ508_001810"
     CDS             3677..4333
                     /locus_tag="VQ508_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2490770.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4441..5536
                     /gene="prfB"
                     /locus_tag="VQ508_001811"
     CDS             join(4441..4512,4514..5536)
                     /gene="prfB"
                     /locus_tag="VQ508_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2490771.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5554..6246
                     /gene="ftsE"
                     /locus_tag="VQ508_001812"
     CDS             5554..6246
                     /gene="ftsE"
                     /locus_tag="VQ508_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2490772.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6239..7165
                     /locus_tag="VQ508_001813"
     CDS             6239..7165
                     /locus_tag="VQ508_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490773.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2162 a         1265 c         1643 g         2254 t
ORIGIN      
        1 agtataagaa cctatcaaaa agtgagggaa aaccctcgga attataaata gaaagagtga
       61 atcttatgct ccaacaactc gtaaatggtt tgattctagg tagtgtttac gcgctgttag
      121 ccctaggata taccatggtt tacggaatta tcaagctcat caacttcgcc catggtgata
      181 tttatatgat gggagccttt atcggttatt tcttgatcaa ttctttccaa atgaatttct
      241 ttgtagcgct tattgtagct atgctagcga cagctattct tggtgtcgtg attgagtttc
      301 ttgcttaccg acctttgcgc cactctactc gtattgctgt tttgattacg gctattgggg
      361 tttctttcct attggagtat ggaatggtct atctggttgg tgccaatacc cgtgccttcc
      421 ctcaagcgat tcaaacagtt cgatatgatt tgggaccaat tagcttaaca aatgtgcagt
      481 taatgatttt ggccatttcc ttgattttga tgattttgtt acaagtcatt gtccaaaaga
      541 ctaagatggg gaaagccatg cgtgcagtat cagtagatag cgacgcggcg caattgatgg
      601 ggatcaatgt aaaccgtacg attagcttta ccttcgcttt gggttctgct cttgcgggtg
      661 cggctggtgt tctgattgct ctttattata actctcttga gcctttgatg ggggttactc
      721 caggtcttaa atctttcgtt gccgcagtac ttggtggtat cggaattatt cctggtgcgg
      781 ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc gactgccttt gggatgtcag
      841 atttccgtga tgccattgtt tatggaatct tgttgttgat cttgattgtc cgcccagctg
      901 gtatccttgg taagaatgtg aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta
      961 atattctatg gttactcctt ttgttagctg gctatagctt gattagtgta ctggtttcag
     1021 tcggagtact taatctattc tatgtacaga ttttacaaca aattggaatt aatattattt
     1081 tggctgttgg tctcaactta atcgttggtt tttcaggaca attttcactt ggtcatgctg
     1141 gtttcatggc gattggtgcc tatgcagcag ctattattgg ttctaaatta ccaacctacg
     1201 gtgccttctt tggagctatg cttgtagggg ctttgctttc aggagcagtt gccttacttg
     1261 tcggcattcc aaccttgcgc ttgaaggggg actatcttgc ggtagcaact ctaggtgttt
     1321 ctgaaattat ccgtatcttt atcatcaatg gtggaagtct tacaaatggt gcggcaggta
     1381 tcttagggat tcctaacttt acaacttggc aaatggttta cttctttgtc gtgattacaa
     1441 ccattgcaac cttgaacttc ttgcgtagcc caattggccg ttcaaccctc tctgttcgtg
     1501 aagatgaaat cgctgctgag tcagttgggg ttaatacgac taaaattaaa atcattgctt
     1561 ttgtctttgg tgccattact gcaagtattg ctgggtcact tcaggcagga tttatcgggt
     1621 ctgttgtacc gaaagattac accttcatta actcaatcaa cgttttgatt attgttgtat
     1681 ttggtggact cggttccatt acaggtgcga ttgtttcggc tattgttctg ggaattttga
     1741 atatgcttct ccaagatgtt gctagtgtgc gtatgattat ttacgcttta gccttggtat
     1801 tggtaatgat tttcagacca ggtggactcc ttggaacgtg ggaactgagc ctatcacgtt
     1861 tctttaaaaa atctaagaag gaggaacaaa actaatggca ttacttgaag taaaacagtt
     1921 aaccaaacat tttggtggtc taacagctgt tggagatgtg actcttgaat tgaacgaagg
     1981 ggaactggtt ggattaatcg gtccaaacgg agctgggaaa accacccttt tcaacctttt
     2041 gaccggtgtt tatgaaccaa gcgagggaac agtaacccta gatggtcacc ttttgaatgg
     2101 gaaatcacct tataagattg cctctttggg acttggacgt actttccaaa atatccgtct
     2161 ctttaaagat ttaacagttt tagataatgt tttgattgct tttggaaacc atcacaaaca
     2221 gcatgttttt actagtttct tacgcttacc agctttttac aagagtgaaa aagaattaaa
     2281 ggctaaagct ttggaattgt tgaaaatctt tgatttagat ggtgatgcag agactcttgc
     2341 taaaaatctt tcctacggac aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga
     2401 acctaaaatt ctcttcttag atgaaccagc agcaggtatg aacccacagg aaacagccga
     2461 attgactgag ttaattcgtc gtatcaaaga tgaatttaag attacaatca tgttgattga
     2521 acacgatatg aatctggtca tggaagtaac agaacgtatc tacgtacttg aatatggccg
     2581 tttaattgct caaggaactc cagacgaaat taagaccaat aaacgcgtta tcgaagctta
     2641 tctaggaggt gaagcctaat gtctatgtta aaagttgaaa atctttctgt gcattacggt
     2701 atgatccaag cagtccgtga tgtaagcttt gaagttaatg aaggagaagt tgtttccctt
     2761 atcggtgcca acggtgcagg taagacaact attcttcgca ccttgtcagg tttggttcga
     2821 ccaagttcag gaaagattga atttttaggt caagaaatcc aaaaaatacc agctcagaaa
     2881 atcgtggcag gtggtctttc acaagttcca gaaggacgcc acgtctttcc tggcttgact
     2941 gttatggaaa atcttgaaat gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct
     3001 aacttgaaga aggttttctc acgctttcct cgtcttgaag aacgtaagaa ccaagatgca
     3061 gctactcttt caggagggga acaacaaatg cttgccatgg gacgcgctct tatgtcaaca
     3121 ccaaaacttc ttcttttaga tgaaccatca atgggacttg ccccaatctt catccaagag
     3181 atttttgata tcattcaaga tattcagaag caaggaacaa ccgtcctctt gattgaacaa
     3241 aatgccaata aagcacttgc aatctctgac cgaggatatg tattggaaac aggaaaaatc
     3301 gtcctatcag gaacaggaaa agaactcgct tcatcagaag aagtcagaaa agcatatcta
     3361 ggtggctaaa acaatccagt ggattgtttt agtcggcaga tgaagattac gaagtaatca
     3421 tcaatatagt ccgggggccc tttttagtcg gtagattgag attgcaaaca aatctgcatc
     3481 tacattgaaa gcttaatttc taataattga aaaaatcgaa tgaaaagttt cttaccttca
     3541 ttcacagagc tcgattttag agctcttttt gctagcttat tcatactttt ctaaattttg
     3601 aaaaagaaat gtaagcgttt gatagatttg caaaaagatt gtataatagg gataagaata
     3661 gaaaaggaga agtctcatgg cagttaaaga ttttatgacc cgcaaggtag tttatattag
     3721 tccagataca acagtatctc atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct
     3781 gcctgttatc gaaaatgatc aattggttgg tttggtgact gagggaacca ttgcacaagc
     3841 aagtccatct aaagcaacaa gtctttctat ctatgagatg aattatcttc tgaataagac
     3901 aaaagtaaaa gatgtcatga ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga
     3961 agatgcaact tatctgatgt tgaaaaataa gattggtatt ctccctgtcg tagataacca
     4021 tcaagtatac ggagttatta ctgaccgtga cgttttccaa gcctttcttg aaattgcagg
     4081 ttatggcgaa gaagggattc gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg
     4141 aaaaattgtt tctttgattg tagaagaaaa tttgaatatc tcccatacag tcaatattcc
     4201 gcgtaaggat ggtaaggtga ttatcgaagt gcaaatcgat gaatcaattg atttaccagc
     4261 cttgagagaa aaatttgaag caaatggtat tcaagtggaa gaaatcgctc gcacttcagc
     4321 aaaagtcttg taagaaggga agtccaaagg cttctttttt catgaaaagg ggattagagc
     4381 aaaagatgga aagaaatgat aaaatatgct ataatgaaat aatgtaaaaa aggagtattt
     4441 atggacattt cagtaattcg tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc
     4501 agggggtctc tttgacctcg aagggctaga ggaagagatt gccatcttgg aaaacaagat
     4561 gacagaacct gatttttgga acgataatat tgcggcccaa aaaacgtcgc aagaattgaa
     4621 tgaattaaaa aacacttaca ataccttcca taagatggaa gagttgcagg atgaagtcga
     4681 aattttattg gattttttgg ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt
     4741 agccgaactt gataagataa tgaccagcta cgagatgact ctactcttgt cagaacctta
     4801 tgaccacaac aatgccatct tggaaatcca tccaggttct ggtggtactg aggcgcagga
     4861 ctggggtgat atgttgcttc gtatgtatac tcgttatggt aatgctaaag gctttaaagt
     4921 ggaagtgttg gattaccaag caggtgatga ggctggtatt aagtcggtaa ctttatcatt
     4981 tgaagggcct aatgcctatg gtctcctcaa gtcagaaatg ggtgttcacc gcttagtgcg
     5041 aatctcacca tttgactctg ccaaacgtcg ccatacctct ttcacatctg tagaagtgat
     5101 gccagaattg gatgatacta ttgaagtgga aatccgtgaa gatgatatca agatggatac
     5161 cttccgttca ggtggtgccg gtggacaaaa cgtcaataag gtttcaacag gtgtacgttt
     5221 aacccacatt ccaactggaa ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa
     5281 tagagatcgt gccatgaaga tgttgcaggc taagctctat caaatggagc aagataagaa
     5341 ggctgcggag gtagattctc tcaaaggtga gaaaaaggag atcacttggg gaagccaaat
     5401 ccgttcttat gtcttcacgc cttatactat ggtaaaagat caccgaacta gctttgaggt
     5461 tgctcaggta gataaggtta tggatgggga cctagatggt tttatagatg cttatctcaa
     5521 gtggcgaatt agctaagata gaaaggaact cacatgtcaa ttattgaaat gagagatgtc
     5581 gttaaaaaat acgacaacgg aacgactgct ctacgcggtg tttcggtcag cgttcaaccg
     5641 ggggaatttg cttacatcgt aggaccttca ggagcaggga agtcaacttt tattcgttct
     5701 ctgtatcgtg aagtaaaaat cgataaagga agtctatcag ttgctggttt taatctggtt
     5761 aagatcaaaa agaaagatgt cccgcttcta cgtcgtagtg ttggggttgt cttccaagat
     5821 tataaattgt taccaaagaa aactgtctat gaaaatattg cttacgctat ggaagtaatc
     5881 ggggaaaatc gccgtaatat caaaagacga gtgatggaag ttttggactt ggttggattg
     5941 aagcataagg ttcgttcttt cccaaatgaa ctctcaggtg gggagcagca gcgaattgcg
     6001 attgcgcgtg caattgtaaa taatcccaaa gtattgatag ctgatgagcc aacaggaaat
     6061 ctggatccgg ataattcatg ggaaattatg aatctcttgg aacggattaa cctacaaggg
     6121 acaactattt tgatggcgac tcataatagc cagattgtaa ataccttgcg ccaccgtgtc
     6181 attgccattg aaaatggccg tgtcgttcgt gacgaatcaa aaggagagta tggatacgat
     6241 gattagtaga ttttttcgcc atttatttga agccttaaaa agtttgaaac gaaatggttg
     6301 gatgacagta gctgctgtca gttcagtcat gattactttg accttggtgg caatatttgc
     6361 atctgttatt ttcaatacag cgaaactagc tacagatatt gaaaataatg tccgtgtagt
     6421 agtttatatc cgaaaggatg tggaagataa tagtcagaca attgaaaaag aaggtcaaac
     6481 tgttacaaat aatgactacc acaaggtata tgattctttg aagaacatgt ctacggttaa
     6541 aagtgttacc ttttcaagta aagaagaaca atatgaaaaa ttaaccgaga taatgggaga
     6601 taactggaaa atctttgaag gagatgccaa tcctctctat gatgcctata ttgtagaggc
     6661 aaacactcca aatgatgtaa aaactatagc cgaagatgct aaaaaaattg aaggtgtctc
     6721 tgaggttcaa gatggcggtg ccaatacaga aagactcttc aagttagctt catttatccg
     6781 tgtttgggga ctagggattg ctgctttgtt aatttttatc gcagttttct tgatttcaaa
     6841 taccattcgt attaccatta tttcccgcag tcgcgaaatt caaatcatgc gcttggttgg
     6901 agctaaaaac agttatatcc gtggaccgtt cttgttagaa ggagccttta tcggtttatt
     6961 gggagctatc gcaccatctg ttttggtctt tattgtttat caaattgttt accaatctgt
     7021 caacaaatcg ttggtagggc aaaatctatc catgattagt ccagatttat ttagtccgtt
     7081 gatgattgcc ctactatttg tgattggggt tttcattggt tcattgggat caggaatatc
     7141 catgcgccga ttcttgaaga tttaggtaaa atagctgctt ttatgaggag attgtaaaat
     7201 ctcctttttt gctacaagag tttttgaaaa gagatgcgta gaagaaaaga gcttccaaag
     7261 aagtccccca gagaagactt cttgatggct aatttgaact tgaaatttgg cgcactagaa
     7321 attt
//
LOCUS       DAVBCR010000085         7061 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCR010000085 DAVBCR010000000
VERSION     DAVBCR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.085"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ508_001814"
     CDS             complement(52..1113)
                     /locus_tag="VQ508_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2490774.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ508_001815"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ508_001815"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2490775.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ508_001816"
     CDS             4069..5013
                     /locus_tag="VQ508_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2490776.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ508_001817"
     CDS             5025..5708
                     /locus_tag="VQ508_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2490777.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5963..6994
                     /locus_tag="VQ508_001818"
     CDS             5963..6994
                     /locus_tag="VQ508_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2490778.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYCLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT         1990 a         1665 c         1437 g         1969 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagcccatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaatacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgacacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccatcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggttcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagccct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg tcccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctatc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtctctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac gcctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtgatc tgcgatagac aggtctgaaa
     2641 tttctccgat atgtggccct tgctcaccac ccacacggac agacaaggcc tggtcattga
     2701 gacgataacc gtgacaggtt ccgcaagtca gctcattcat gtagaggcgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acgccaccaa attcattctc atagtggaaa tggaatttct
     2881 taccatctga gccgtagaga atcaagttct tgtcttcttc tgacaagtcc tcaaaaggct
     2941 tatccatagc cactccaaaa gccgtcatgg cctgctctag catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatttggca
     3061 ctaccaaatc agtatccacc tccagcttga tgcccaagcc gtcacactca ctacaagagc
     3121 caaaaggagc attgaaagaa aagagacgag gctctaactc tgggacagta aaaccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtt gtccatggtg tcgataatga
     3241 cataaccttc tgcaatacga agggcagcct caatggaatc aaagagacga ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acggacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 acaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc cttgatagcc ccatgcccgt tgatacagta aggcgtcccc acacgcgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgttgttcc caccgtcgag cgagggtttt
     3661 tgctagtcgt tttctggtcg atagaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccaaact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat tcccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taaaaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctgt gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
     5761 agttatactc aatgaaaatc aaagagcaaa ctaggaagct agctgcaggt tgctcaaagc
     5821 acagctttga ggttgcagat aaaactgacg tggtttgaag agattttcga agagtattaa
     5881 aagtattctt ctgaaatccc acatagcttt ctcttatatt ttgtgataaa ataggctcaa
     5941 tctatttcta ggaggatgag atatggtttc tactattggt attgttagtt tatctagtgg
     6001 cattatcgga gaggattttg tcaaacacga agtggacttg ggtatccaac gtctcaagga
     6061 tctgggactc aatcccatct ttttgcccca ttcgttaaaa ggattagact ttatcaagga
     6121 ccatcctgaa gctcgtgcag aggatttgat tcatgccttt tctgatgata gcatcgacat
     6181 gatcctatgt gccatcggtg gagacgatac ctattgcttg ctaccttatc tttttgaaaa
     6241 tgaccaactc caaaaggtta tcaagcaaaa aatttttctt ggcttctcgg atacaaccat
     6301 gaaccacctc atgttgcata aattaggaat caagactttt tatggtcaat cctttttagc
     6361 agacatttgt gaattagaca aagaaatgct agcctatagc cttcactact ttaaagaatt
     6421 gattgagacg ggaagaattt cagaaatccg ccctagtgac gtttggtatg aggaacgaac
     6481 tgattttagt cccacggccc tgggaacacc tcgtgtcagt catacaaata caggttttga
     6541 cttgttgcaa ggaagggcac tattcgaggg gaaaatcctc ggtggttgcc tcgaatccct
     6601 ctacgatatc tttgacaact ctcgatacgc agatagcacg gagctctgcc aaaaatacaa
     6661 acttttccct gacttgtcag actgggaagg gaaaatcctc ttgctcgaaa caagcgaaga
     6721 aaagcctaag ccagaagact tcaaaaagat gttgctaact ttaaaggaca ctggcatatt
     6781 cgcggtcatc aatggactct tggtcggaaa acctatggat gaaactttcc atgacgacta
     6841 taaagaggca ctattggata tcattgacag caatatcccg attgtctata atctgaatgc
     6901 cggccacgca actccaagag caattgtacc ctttggggtc catgctcatg tagatgcaca
     6961 ggaacaagtc attcgctttg actataacaa ataaagctgg gaaaagtttc tcagcttttt
     7021 tctcggtatc attactggca ttttcatcac atattacagc a
//
LOCUS       DAVBCR010000086         7013 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCR010000086 DAVBCR010000000
VERSION     DAVBCR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.086"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQ508_001819"
     CDS             complement(317..2008)
                     /locus_tag="VQ508_001819"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2490779.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQ508_001820"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQ508_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2490780.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQ508_001821"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQ508_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2490781.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..>7013
                     /locus_tag="VQ508_001822"
     CDS             5322..>7013
                     /locus_tag="VQ508_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490782.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1901 a         1370 c         1667 g         2075 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaa
//
LOCUS       DAVBCR010000087         6989 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCR010000087 DAVBCR010000000
VERSION     DAVBCR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.087"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQ508_001823"
     CDS             226..6657
                     /locus_tag="VQ508_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2490783.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2759 a          943 c         1347 g         1940 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 ttagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgat
//
LOCUS       DAVBCR010000088         6912 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCR010000088 DAVBCR010000000
VERSION     DAVBCR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.088"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(267..1409)
                     /locus_tag="VQ508_001824"
     CDS             complement(267..1409)
                     /locus_tag="VQ508_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2490784.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1575..2195)
                     /locus_tag="VQ508_001825"
     CDS             complement(1575..2195)
                     /locus_tag="VQ508_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2490785.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2199..3479)
                     /locus_tag="VQ508_001826"
     CDS             complement(2199..3479)
                     /locus_tag="VQ508_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2490786.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3540..5024)
                     /locus_tag="VQ508_001827"
     CDS             complement(3540..5024)
                     /locus_tag="VQ508_001827"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2490787.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(5100..6377)
                     /locus_tag="VQ508_001828"
     CDS             complement(5100..6377)
                     /locus_tag="VQ508_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2490788.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6484..6567)
                     /locus_tag="VQ508_001829"
     tRNA            complement(6484..6567)
                     /locus_tag="VQ508_001829"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6531..6533),aa:Leu,seq:caa)
     gene            complement(6577..6648)
                     /locus_tag="VQ508_001830"
     tRNA            complement(6577..6648)
                     /locus_tag="VQ508_001830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6614..6616),aa:Gln,seq:ttg)
     gene            complement(6662..6734)
                     /locus_tag="VQ508_001831"
     tRNA            complement(6662..6734)
                     /locus_tag="VQ508_001831"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6699..6701),aa:His,seq:gtg)
     gene            complement(6749..6819)
                     /locus_tag="VQ508_001832"
     tRNA            complement(6749..6819)
                     /locus_tag="VQ508_001832"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6785..6787),aa:Trp,seq:cca)
     gene            complement(6824..6904)
                     /locus_tag="VQ508_001833"
     tRNA            complement(6824..6904)
                     /locus_tag="VQ508_001833"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6868..6870),aa:Tyr,seq:gta)
BASE COUNT         2095 a         1619 c         1226 g         1972 t
ORIGIN      
        1 ctataaaata gattgcaaga atgttgctta gattattaca agatgataac tttccatttt
       61 ccattcaaat tgatactcaa tgaaaatcaa aaagcaaact aggaagctag tcgcaggttg
      121 ctcaaagcac agctttgagg tttcagataa aactgacgtg gtttgaagag attttcgaag
      181 agtatgacaa agaaaaaacg aacgagaatc gcttcccatc acgaaagcta gatctcattc
      241 gtcaaaatga tgttaccaag tcgggtttat ccattcattg aaacgtgaac gtgatcatag
      301 tgattttctg tcacactacc acggtctggc attgggttcc aagtgttagc tggcccatat
      361 ttgctatcga atggagcata gaaacgttgt ttccagatga tgtaactaat gccacggctg
      421 gccatgtttt gaatagcata ttccgcaatc ttatccccta attctgaacg ttctggtacc
      481 ataaagtcga tagccaaacc ttttccgtga tctccactgt ctcctggacg ataaccgcta
      541 aaggatgtaa tgccaaacaa gttagcaatt tcttctttaa aggcagctgt ttgtggttga
      601 agacctgcat tttcagattt tgctactgca agtccagcat aatcgggcgc agctggagcc
      661 tcgtaagttg ttgaaattat tttcgtctct tctggttgat aagtagaaac tgctgctgta
      721 gctccatttg atgatgctac ttcttttgct tctgaacttg ttgtagttgc ttccactgct
      781 gatgcagctt gagggcttgc ttgtgtttcc tgcttctcag ctggagtcgt tccctgagga
      841 gcttcttctg caactgcact gcttgtttcg gccgtttgct cctctgggac agaagtgccc
      901 gtagatggtg ccacttcttc agaagcaatc actgtctttg taacttctga acttgatgct
      961 acttcttttg gagcttctgc aattggttga gaaaggtctg caacctgaac agtttgatca
     1021 tcaacggtca cttgattagt ggtcaaatct gctgtcgcag ttgtcacttc ttcactagag
     1081 tctgcttgag gtgtttggat ttcaacttct gttacttctt ctgcttcatt gacagtcgtt
     1141 gtcaaaacag tttctgggaa aatcaagtcc atattagtga ttttgttcag attcgcaagc
     1201 actgtgacat ctacacccaa ggcttctgca atggtgctca aagtatcacc atactgtacg
     1261 gtataacttg ttttgttgtc cgttttagtc aaatcgtttt ggatttgctc aacactacgt
     1321 gcagtccaaa gaacttcttc tgcttgagtt gccaatactg gggcaaatga caaggctact
     1381 gttgacgcta ataacattct tttcttcatt ctttcaaatt cctttcaaat gagtacctgt
     1441 ctatcataac acaaaaaatg tagaaaaaaa gccctctcgg gcctatttaa cagaactgtc
     1501 tattccttgt aacaaactag attacttgaa atagtttgtt aggtctctgt cacaaaactg
     1561 acacaatctt tttatcactt agtctcaaaa atacgggaaa tatctgctaa cgcttgaatc
     1621 ctgtgattcc cttcataagt agactctaaa aagttgatac tttgaatccc actattctgg
     1681 gcaaattcca catccagagt ccgatcccct atataataag tattatcaga attcaactga
     1741 tacttatcta gcagataggt agccgcttct ggacttggct tccgcacaaa gccactctga
     1801 ctggtcaaaa tctctgtaaa ataggattcc acccccaagt ccttgagaat ggtaaaagtg
     1861 ttgttcccct tatgagtata tataaactgc tgaattcctg attcgtctgc ccaagctagc
     1921 acctcacgcg cacctggcat caaaactacc tgagcattct tctcagccag actctgggca
     1981 cgcacctgat ttagcacctc aacatccaga tttctatctt ctgccacccg cacaagcaaa
     2041 ttttgcaccg aatacttgaa gataaactct ctcaccttct ccttatcata aggaatagaa
     2101 aactgagcaa aagtctcctc aatccctgat aaaatcgctt cgtaagagtc caataaagtc
     2161 ccgtctaaat cccaaataaa agctgttttt tgcatttcct atcctttcaa gctcatatag
     2221 cgctggtaac gatagcgtag aaataaccag cgaaaaccat tatccaagag agaccctgcc
     2281 caaataccag gcaagcccca accaagcaca atacccatca gatatcctgt cccaatgcgg
     2341 atacaccaca tgcctatact tgtcgcataa aaagggaggc gtgcatttcc taatccctgc
     2401 cagactgccg tatagatgac tgttcctgtc gtcatagggg taccaagtag tgaaaacagt
     2461 gtcactagaa cactagcctc caccgctaga gaatcagtcg tatagagatg agttaatggt
     2521 acacccaaga catatatact aaaggacagg ggcaacatga ggaacagaga aagccaaaag
     2581 gtttgtttac tcaaactagc aactcttttc caatcatcct ctccaactgc tcgggccaac
     2641 agcatgaccg ttgccgtagc gacaccaaag gcaggcatat agttaaactg ggtcaagact
     2701 tctccgattg cattcccagc aactgcctcc gtcccaaaag aaacgaccaa ggcaatgatc
     2761 actacatctc cagccctcat cataagtcgc tctccagctg ctggtaaagc caaggtcaac
     2821 agttccttat ctaaaccaaa agttagcttc ccataaggca gttttaattg tgaccacaaa
     2881 atcacaagac caaccaaacg agacagaatt gtcccccaag caacaccagc tatccccata
     2941 tccagaacaa aaatagctag acttgaaaaa agaatattca aggcattgga taaaaaacta
     3001 acatagagag gcagacgtgg attatgcgtt gcacgaatca aggctcccaa actagtcatt
     3061 aaacctaaga gaacaatcga tccgcctacc aaagatagat acagtccacc actctcagct
     3121 acatccctct ccgtccccaa aagtcctatc atctctttcc cagcgaagat ggacaaaaat
     3181 cctaaaagga aacttaatag taaggtaatc ttcaacgcct cagtcacatg ataggccaac
     3241 ttcgactggt ctttctgccc tatgcttttt gaaataacac tggaaatagc agctcccaga
     3301 gcgatgaaaa tcgcctgata aatggtgata atattaccag ctactgaaac ccctgaaata
     3361 gctatcaatc ctaaatgagc aaccaaataa ctgtccacca ttcccattag catctgcaaa
     3421 aagttttcac ccatagctgg caatgcaata ttaagaatgt ctttattttt cttaaacaat
     3481 ctctcctcct gatgaaaaga aactcagttg gtttcccaac cgagtttgct ccctctgtct
     3541 taaagtccta aataagcctc aaccgctgct tgcatgtcag cagctgccac tgttgtcttg
     3601 tgacgaattg gagctatttc aagcccatca actgctggtg gcactgcaac gcctgagatt
     3661 tcatgtaatt gagccaaggc ttcaaagtct gtcaaacctg cttttccagt tacagcttct
     3721 actgcaacta ctgggaactt gtatggacta gctgttgaag caatcactgt cttagttaca
     3781 tctccagtaa ccgcttggta ttttctataa actgctgagg caaccgccgt atgtggatcc
     3841 tcaatataag aatctgactc ataaacacgc ttgatttctg ccgctgtttc ttcctcagtc
     3901 gcatattcag ctgcaaagag gtccaaaatc tctgcatcaa agtctgtcaa cttatattgt
     3961 ccttgcgtgt tcaaggcatt cataagttca gctgtctttt cagcattatt tcccaaaaga
     4021 tggaaaatca agcgctccaa gtttgaagat accaagatat ccatagatgg gctggttgtt
     4081 accttaaact cacgtttttt gtcatagaca cgtgttttaa agaagtctgt caagacatta
     4141 ttgtcatttg aagcacagat tagcttgcca actggtaggc cgatttgttt ggcataaaag
     4201 gcagccaaga tatttccaaa gtttcctgtt ggtactgtga agttaacctt ttcaccagct
     4261 acaatttcac cagtcttaac caattgagcg taagcataaa cataataaac aatttgtggc
     4321 accagacgac caatgttcat agagttagct gatgaaaatt gcaacttgtt ggtagtcaat
     4381 ttttcacgaa gagccacgtc gttaaacatg tgcttcacat ttgtttgcgc atcgtcaaaa
     4441 ttaccatcaa tagcaataac atgagtattg tcaccattct gagtggtcat ttgcaactct
     4501 tgtaccttgc tgacaccacc ctttggataa aagacgataa tctcagtacc aggcacatcc
     4561 gcaaaccccg ccatagcagc tttccccgtg tcaccagatg tcgctgtcaa gataacaatc
     4621 ttgttctcca aaccatgttt cttagcagca gtcgtcataa agtatggcaa aatagacaag
     4681 gccatatcct taaaggcaat cgttgaacca tggaaaagtt ccaaattgta ttgcccatct
     4741 aatttcacta atggtgcaat agctggagta tcaaatttgc tatcgtaggc attgttgata
     4801 cagtagtcca actcctcagc tgtaaagtca tctaaaaatg ctgacaaaac tagcttagca
     4861 acttcctggt aagaagcatc tttcaatttg tcaaagtcca aatctacctt tggataagta
     4921 accggtgtaa acaaaccgcc gtccgtcgcc aaaccttgca aaattgcttg gctggcagtt
     4981 actgtattgt tggcatcacg cgttgattga taaactaatg tcattactct ctatccttta
     5041 tctatagtct cgtccattat atcatgattt ttcagaaaat tctcccttta taagagaaat
     5101 tataggctca attcttctct caaaggctgt aaataggtca tttcttcctt atttctcacc
     5161 actagtccct cctcagctag taggagtaag tctttggaaa attcaataat cgtagtttct
     5221 tcctcatctg taagattttt cttagaaaat tgtctcctta aagacttgta atcataacca
     5281 aatactttaa agaaaggtgc tgtttctaag taagcttcta acttgtctaa attaaccaat
     5341 aatcccaagt gaaaagctgc agaagcaaaa gtcctatcaa gtggctgtgt acacacacta
     5401 cgaaactcaa ctgttcctcg agtcgttaaa tcttggtact ggtaactacg atgagtttca
     5461 aaatccttct cttgggggta aataataacc tcatccccat tcagagcaaa tgcttggatt
     5521 tcggacgtag ccaaatagtc cccagcctga ataggataaa aataataggt ctgcccatca
     5581 cgttccgcag taaaaatcgc agaatgattt agatagtcaa aaaaatcagc ttcatcatta
     5641 aggagtctag cattgacccc aacattctct ggatagatac catgcataga ttcttcccag
     5701 aaaatatccc ttgaaatttt cgtatcccaa tccgcacccg aaaattcaga gtttgcaaat
     5761 aaataagcct tagccgcttc aatttgagta aaagcattaa tcacccgtaa gtagttggtt
     5821 tttgaaatat ccagctgaac ctggctccca cagataaaag taccatattc agggaaatga
     5881 tgtaaatctg atttaataat atttctactc aaattcaaat aatccatcaa catctgatag
     5941 cgtggataag ccactggaca attctcattt ttatcccagt tgggatggat accacagcca
     6001 acaatagcat gatttgattc agctaacttt ctctgaatta cattcatata attattgaaa
     6061 cgattttcga cctcttgaat cgtttcagcc ttaccaaatg caaactcaat cgttgtatag
     6121 gaaacttcaa ataaaatagc atcctgactt atcggatcta ctaactggat cagattgcca
     6181 aaatcatcta cctttgctac ggtgagatcc aaagtagaaa ctaaataatg aaatagatcc
     6241 tttataactt caacatctgt agcatcccct tctaaacttg caacaggata ctccaactca
     6301 attccgacaa acaattcagg attttcttta atatttttca aataccgatg ttttaataaa
     6361 tcgatagaac aagacatact accctacact ttcttaatct aaataaaatt tgaataacta
     6421 taattatacc aaaaataata taaaaaagga acgaagaata acaacgttcc taacactata
     6481 ctataccggc ggccggggtc gaaccggcac gtccttgcgg acactggatt ttgagtccag
     6541 cgcgtctgcc aattccgcca cgccggcaaa tagtaactgg ggtagctgga ttcgaaccaa
     6601 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaat aatataaaat
     6661 aggcgagtga tggggatcga acccacgcat gccagagcca caatctggtg tgttaaccac
     6721 ttcaccacac ccgccataat tctattaaca cgggcagtag gaattgaacc cacactgaag
     6781 gttttggaga ccttagttct acctttaaac tatgcccgta aaatggaagg ggagggattc
     6841 gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc ttcgctaccc
     6901 ttccaaaata ta
//
LOCUS       DAVBCR010000089         6742 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCR010000089 DAVBCR010000000
VERSION     DAVBCR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.089"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..88
                     /locus_tag="VQ508_001834"
     CDS             <1..88
                     /locus_tag="VQ508_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2490789.1"
                     /translation="STTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(85..330)
                     /locus_tag="VQ508_001835"
     CDS             complement(85..330)
                     /locus_tag="VQ508_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2490790.1"
                     /translation="MEQSPQKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(345..1517)
                     /locus_tag="VQ508_001836"
     CDS             complement(345..1517)
                     /locus_tag="VQ508_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2490791.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(1576..2421)
                     /locus_tag="VQ508_001837"
     CDS             complement(1576..2421)
                     /locus_tag="VQ508_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2490792.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            2546..3103
                     /locus_tag="VQ508_001838"
     CDS             2546..3103
                     /locus_tag="VQ508_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2490793.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3122..3589
                     /locus_tag="VQ508_001839"
     CDS             3122..3589
                     /locus_tag="VQ508_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2490794.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            3678..4307
                     /gene="upp"
                     /locus_tag="VQ508_001840"
     CDS             3678..4307
                     /gene="upp"
                     /locus_tag="VQ508_001840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2490795.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4481..5071
                     /gene="clpP"
                     /locus_tag="VQ508_001841"
     CDS             4481..5071
                     /gene="clpP"
                     /locus_tag="VQ508_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2490796.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5150..5398
                     /locus_tag="VQ508_001842"
     CDS             5150..5398
                     /locus_tag="VQ508_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2490797.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5499..6659
                     /locus_tag="VQ508_001843"
     CDS             5499..6659
                     /locus_tag="VQ508_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2490798.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         2105 a         1305 c         1406 g         1926 t
ORIGIN      
        1 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgagtgctgg
       61 tattatcaat catgaactag gattctagtt ttgcaggaag tctaatagag tatgaacctg
      121 ttctcgtatt tcttcgagag ctaagttact ttcaagaata atatttccac tttgaatata
      181 agttcgaact tgttgaaaat cagcttctgt caagatatct actagataag acattttagg
      241 gatagcattt tttccattag gagtgactcc ccttagcaag ataatatgtt ccaaagtagt
      301 tcctttcttt ttttgggggg attgttccat ttagtaatgt atatttagat tggatttgat
      361 cccgccaccc agccggtaca gagggcagaa gtgatgttaa agccacctgt gtgggcattg
      421 atatccataa cttcgcctgc aaagtggagg ccaggtacca gcttactttc aagggtttta
      481 ggattgattt ccttgagact gactccaccc ttggtaacaa aggactttgc aagggacatt
      541 tttccagtta caggaatttt aagttcttta atggactgga caagttgttc tcgttccttt
      601 tcagtcagtt gtttgacttt ttcaggatat ccttgtacaa aaaattcggc caagcgttct
      661 ggtaacaagg tttttaaagc gtttttcaag gatttttccc gattttcttc tagaaatgta
      721 accaagtcct tctcagaaag ttgaggcaaa acatcgagtg agagaacctc cccacctttg
      781 acaaagctag acatgcgtag ggcagcagga cctgacaaac caaagtgggt aaagagtaaa
      841 tcatgagtga tgacatgctt accataactt agggtcacat cgtccaaaga aatcccttgt
      901 aaggctttat gtggaaaatc tgttaataaa ggactttcag cagcctcaag atcggtgatg
      961 gtatgcttaa aatggcgagc aatctcgtga ccaaaaccag tcgaaccagt cgaaggataa
     1021 gacttaccac ctgttgtgac aatgagtttc tcacaagtga aggtttgatc cgctgactta
     1081 aggacaaact ggtcatctac ttttttaaca gaaacgattt ctatttgagt agcaacttga
     1141 ccacctagtt cggtgatttt cttttccaaa gcttcgataa tagtccgaga cttgtcactg
     1201 gctggaaaga cgcgtccgtg gtcttcgacc ttaagtttaa caccattttc tgtaaaaaag
     1261 ttgatgatgt catgattatc gaactgggag aaaacactgt aaagaaagcg tccgtttcca
     1321 ggaattccag ctagcaggtt gtctaagcta ccattgttgg tcacattgca acgtccccca
     1381 ccagtcccag ctaatttttt tccaagtttc cgattttttt cgatgaggag ggttttctgt
     1441 ccataaaagc tactggaaat cgtagccatc ataccagcag gtcccccacc gatgacaata
     1501 gtatcaaaat gtttcatagc tctattgtac cacaaaaaaa caagagatga tggtcacctc
     1561 ttgtcaagaa tacaattaat caatttcata gcccatcagc aaaccgccat cttctgcata
     1621 gaaactgcag agaccagagg ttggtagaat tttaatatcc gcttgtggga aggtttcacg
     1681 gattcgctct gagagctgtt gacaacattt ttcgttattg cgttgggcca tgacaatacg
     1741 gccaccagca tatccagctt ttactaactc atcataggca gcttgaactg atttctttga
     1801 tccccttgct ttttgtagca attcgagagt tccagtttca ctagcttttc cgaccatacg
     1861 aatgttgaga aggccaacga ccgtaccgat aagcttgctc aaacggccgt tcttcaccaa
     1921 gttatcgact ttggctagga caaagagcaa cttagttttt tcttgatagg cggtgatagc
     1981 ttcaaccact tcttcaaaag acaagccctg gtcaatcaag tcattcaatt tttctacgag
     2041 taggtcaact tcaccaccag cagataaact atcaatcaca tgaatcttag tgtcaggatg
     2101 gtcttccaga taaatattct ttgctagttg agcactattg tgactgccag aaagggtacc
     2161 tgtgatggtt actaggaaaa tgtttttggc accttcaaat gctcgcaaat agtcatctgg
     2221 gcttggacaa gccgattttg aagcttctgc agttgcatac atggtttcca tcatttggtc
     2281 aatatcgaga ctggcgtcat caacaaagac ctgatcagct acttgaatgg ttaaggggac
     2341 acttacaaag gttgtgttaa tagctggtgt tggcagttga cgataatcac aaccagagtc
     2401 agcaataatc ttccaagtca tagaaattct ccatctttgt cagttataca ttgacaaagg
     2461 ttctgtcttt ttttacaatt atatcatgaa aacccttgaa acaaaagcat catccgtctg
     2521 ttgtgacaag tagaaagaaa atgttatgtc tgaacgtaga atctctgaaa agtctcttga
     2581 aaatctcaga aaatcaaacc aagaatccaa tttattaacc agagaagcca ttgaaacagc
     2641 cctcttgcaa ctcttggaaa aaaaggaact gaccaagatt agtatttctg aattggtcaa
     2701 acgtgcaggt gtttcgcgtg cggcctttta tcgcaattat gactccaaag aggaaatttt
     2761 agagagcgtc tttaaacgaa ctgtccacaa tattatggaa cagctgcatc attacgattt
     2821 aaagacagac ctttatttgg tttgggttca ccttttccgg gaggccagaa aggaagctag
     2881 agtaattcaa ttggccttgg attaccatct ggaaaaaatc tttgtccaag ccatgcagga
     2941 atttctagaa aaataccatg ggaaatcaaa aggtgtcagc tcttatcttc attccttctg
     3001 gagctcagcc atcgtctctg tccttctaaa atggatcaag gatggcatga aggtaccagc
     3061 tgaaaagatt gcagatttag gtttaccatt ttttaaaaaa tagagaaaaa ggagaaaaga
     3121 gatgactgaa aaaagactgg cctgggatga gtattttgca gcccaagctc tactaattgc
     3181 gaatcgttcc acttgtaaac gtgctaaagt gggcgcgatt ctggtaaaag ataataaggt
     3241 tatttccact ggttacaatg gttcggtgtc agggactgag cattgtattg atcacgaatg
     3301 tttggtcatt gaaggccact gtgttcgcac ccttcacgct gaggtcaatg ctatccttca
     3361 aggtgctgag cgtggtgttc ccaaaggctt tacagcctat gtaacccatt ttccttgtct
     3421 caactgtaca aaacaattgc ttcaggtcgg ctgcaagcgt gtggtttata tcaaccagta
     3481 ccgaatggac gactacgccc aataccttta tcaagaaaaa gggacagaat tgacccattt
     3541 accacttgag acagtacaga cagctcttaa agaggctgat ctaatgtaaa aattatcaaa
     3601 aataaatggt ttagaaagat ttttaacccg ttttttggta taataagaag aataaattga
     3661 aagaaggaat tccaaaaatg ggaaaaattg aagttattaa tcacccactg attcaacaca
     3721 aattgtcaat cttgcgtcgt acagatactt ctacaaaagc ttttcgtgag ctagtagatg
     3781 agattgcaat gttgatgggg tatgaagtac ttcgtgatct tccactagaa gatgtggaaa
     3841 tcgaaacacc aattacaaaa acagttcaaa aacaattggc aggtaagaaa ttggccatcg
     3901 tcccaatctt gcgtgcaggt atcgggatgg ttgatggtct cttgagcttg gttccagctg
     3961 ctaaagttgg ccacatcggt atgtaccgtg atgaagaaac atttcaacca gttgaatact
     4021 tggtgaaatt gcctgaggac attgaccaac gtcaaatttt tgtagtagac ccaatgttgg
     4081 caacaggtgg ctcagcaatc ttggctgttg attctcttaa aaaacgtggc gcatcaaata
     4141 tcaaatttgt ctgccttgta tctgctccag agggtgtaaa agcccttcaa gaagctcatc
     4201 cagatgtaga aatctttaca gcagccttgg atgaacgttt gaacgaacac ggttatatcg
     4261 ttccaggtct tggagatgct ggagaccgct tgttcggtac aaaataagat cgaaaagaag
     4321 aatgacttgg aaaagtattt ccagtcacga aaggaggttg ggtttttgtt tctgtctaat
     4381 gaaagcagag caaaaatttg accttttttg accaagatat tataatagtc ttatcttcag
     4441 tcatttgacc aacaaaatta aaactcaaaa ggagaaatga atgattcctg tagttattga
     4501 acaaacaagc cgtggagaac gttcttacga tatttactca cgtcttctca aagaccgcat
     4561 cattatgctg acaggtccgg ttgaagacaa tatggctaac tctgttattg cccaactgct
     4621 tttcttggat gcccaagata gtacaaaaga tatttacctt tatgtcaata caccaggtgg
     4681 ttctgtttca gctggtttgg caatcgtaga taccatgaac tttatcaagg cagatgtcca
     4741 aaccattgtt atgggaatgg ctgcatctat gggaacagtc atcgcatcaa gtggagcaaa
     4801 aggcaaacgt ttcatgcttc caaatgctga atacatgatt caccaaccaa tgggcggtac
     4861 aggtggtggt acccaacaaa ctgatatggc tatcgctgca gaacacttgc tcaaaactcg
     4921 taataccttg gaaaaaatct tggctgaaaa ttcaggtcag tcaatggaaa aagtccatgc
     4981 agatgcagaa cgtgataact ggatgagcgc ccaggaaaca cttgaatatg gctttattga
     5041 tgaaattatg gccaacaatt cattgaacta atgatgatag aaggcaaact cgactgggtt
     5101 tgcttttttt ggtataatag ggagagattt cttagaaaga ggatttatca tgtttgaaaa
     5161 agtcaatcgc tctggcttga ttatctatct ttactataat cgtgatgcca aaaaactgca
     5221 ggattatgga gatattacct atcattccaa gaaacatcgt tacttacaac tctatgttcc
     5281 aactcaagaa gtggagcaat tggtcggacg cttgagcaag gaaaaattta ttaaaaaagt
     5341 tagggtttgt catatccaag agttggaaac accctttgtg ggcaatcttt atcgatagga
     5401 aaacgttatc atcgaaaaag ttcaaggaaa acattgacaa ttttctgata attcggtata
     5461 ttcttaacag actatttaag aaataaggag acaaaaagat gaagaaaaaa tttgccctat
     5521 cgtttgtggc gcttgcaagt gtagcacttc ttgcagcctg tgtagaagtg aagtctggag
     5581 cagtcaacac tgctggtaac tcagtagagg aaaagacaat taaaatcggg tttaactttg
     5641 aagaatcagg ttctttagct gcatacggaa cagctgaaca aaaaggtgcc caattggctg
     5701 ttgatgaaat caatgccgca ggtggtatcg atggaaaaca aatcgaagta gtcgataaag
     5761 ataataagtc tgaaacagct gaggctgctt cagttacaac taaccttgta acccaatcta
     5821 aagtatcagc agtcgtagga cctgcgacat ctggtgcgac tgcagctgcg gtagcgaacg
     5881 ctacaaaagc aggtgttcca ttgatctcac caagtgcgac tcaagatgga ttgactaaag
     5941 gtcaagatta cctctttatt ggaactttcc aagatagctt ccaaggaaaa attatctcaa
     6001 actatgtttc tgaaaaatta aatgctaaga aagttgttct ttacactgac aatgccagtg
     6061 actatgctaa agggattgct aaatctttcc gcgagtcata caagggtgaa atcgttgcag
     6121 atgaaacttt cgtagcaggt gacacagact tccaagcagc ccttacaaac atgaaaggga
     6181 aagactttga tgctatcgtt gttcctggtt actataatga ggctggtaag attgtaaacc
     6241 aagcgcgtgg catgggaatt gacaaaccaa tcgttggtgg tgatggattc aacggtgagg
     6301 agtttgtaca acaagcaact gctgaaaaag catcaaacat ctactttatc tcaggcttct
     6361 caactactgt agaagtttca gctaaagcta aagccttcct tgacgcttac cgtgctaagt
     6421 acaatgaaga gccttcaaca tttgcagcct tggcttatga ttcagttcac cttgtagcaa
     6481 acgcagcaaa aggtgctaaa aattcaggtg aaatcaagga taaccttgct aaaacaaaag
     6541 attttgaagg tgtaactggt caaacaagct tcgatgcaga ccacaacaca gtcaaaactg
     6601 cttacatgat gaccatgaac aatggtaaag ttgaagcagc agaagttgta aaaccataat
     6661 agaaaaatgt tgaaataggg aatgagcctt tgactcactc cctgtttcga tatttaatac
     6721 tcttcgaaaa tctcttcaaa ct
//
LOCUS       DAVBCR010000090         6533 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCR010000090 DAVBCR010000000
VERSION     DAVBCR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.090"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            135..425
                     /locus_tag="VQ508_001844"
     CDS             135..425
                     /locus_tag="VQ508_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490799.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            449..1165
                     /locus_tag="VQ508_001845"
     CDS             449..1165
                     /locus_tag="VQ508_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2490800.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1294..2109)
                     /locus_tag="VQ508_001846"
     CDS             complement(1294..2109)
                     /locus_tag="VQ508_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2490801.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2106..3011)
                     /locus_tag="VQ508_001847"
     CDS             complement(2106..3011)
                     /locus_tag="VQ508_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2490802.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            3409..5538
                     /locus_tag="VQ508_001848"
     CDS             3409..5538
                     /locus_tag="VQ508_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2490803.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            5525..5974
                     /locus_tag="VQ508_001849"
     CDS             5525..5974
                     /locus_tag="VQ508_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2490804.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            6033..6305
                     /locus_tag="VQ508_001850"
     CDS             6033..6305
                     /locus_tag="VQ508_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2490805.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         2045 a         1191 c         1419 g         1878 t
ORIGIN      
        1 tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc
       61 ttaaagttta ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata
      121 ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga
      181 aaaggcttac aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag
      241 tcccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta
      301 cgattaaacg tatttcaatt gaataccctg aactctacgc tgtagctcaa acaagtggtg
      361 agattccaga aaaaaacgcg aagaattgcg tcagattatc acaactatct ttgaacaaaa
      421 gatgaataag catagtatta agtagagaat gaaaaaatat tttattggcg gtttgggaag
      481 caatgcctat catagcaagg attttcttca agaactagat tcgcaggtct attttctaaa
      541 tccatatgaa aagcatcttc gagatgaaac agaattgaaa tcatggttta aaaatgagat
      601 tgtagaggaa gaatctatct gtctgatagg tcattctatt ggaggcgatt tagctcgtta
      661 tttcgcatcg gaatttgaag aagtaaagaa actgattctt ttggatggtg gctatctaga
      721 tttagataag attttacctt tggatacaga gttagaggaa actaaaaatt atatcaaatc
      781 tcaaattatt tcggacttag atgttcttac ttctaaagaa aaatctgaag caaagcattg
      841 gtcagaaaat atggagaaag ctgtaagaca gtcctatcac tggaatgttg agtataatag
      901 atatgagttg gctataaatt atgaaaatat agaagcgata ctccgcctac ggagaaaaat
      961 acaagctttt aagagagaag tgggagatac cttgtttatt agtcctcgct atcctaatga
     1021 agctacatgg agagaggaag ccctaaaaga attgccagac tattttgata ctattttttt
     1081 agaaaacttt ggccatgagc tttatactca agcacctaaa gaaatcgcta gtctaatgaa
     1141 tgagtggctc gcttattttc tatgagctac ttgtattgct ggaaatggta taagatgaaa
     1201 atctacaagt cactaatttt catataacaa aaaacgaaca ggctcttttt ctgttattca
     1261 gaagagaact tgttcgtttt tatcttgatt ttcttaaaaa gctctatata catagggatt
     1321 ttctggttta tcgactttgg taaagctgtc gatttccaag gttggaaggt tttgtttgag
     1381 ttctttatgg tagtgattga ccagttttcc tttggctgtt atccaagtgt tgttttcata
     1441 ctgccgggta ttgcccttgg tcagcaatcc atagacacca gaatctgcga tacagtggat
     1501 aatgccgaat cggaacagaa attgactatt ggcatgactg gggtcgttat agacaaagcc
     1561 tgtaaactgg attgtcttgc cctcaaactc atctggatag tcgtagatag cctccatgac
     1621 ttccatatag ttttcattag tgatctgaat actatcttgg gataagtatt tatccgccgc
     1681 cgttcgcatt tccttttcat aggctgattt tgaaaaataa gaactggtat ctggtttcaa
     1741 atattggctt gtcgtccctt cgcttgtctg aatggctaga tctgttcctt ccgatagggg
     1801 gaaatgataa cctttagcag aaacggtctg agaatccaag ctaacagttg ggaaagttaa
     1861 gccgatgaca agtggaatag ccagaagaga aatactcgtc atcttggcca atcggctgtt
     1921 cagatgactg tgggttttga cttgcttcat ccagatatac aattgaacaa ttgccaagat
     1981 aaaggaaagc accatggaga tataggccag ataggaatag tgcatgttga tgtactggtt
     2041 tagtttgccc gacagatgga ggtaaatagt cagttcaaaa tagccagcta aaactaaaaa
     2101 tcgaatcata ggatgactcc aatcaagaga gaatagacta agacgacaag agttacaatc
     2161 gttatgaagt gactgataaa tcgtgctttc aagtaatttt tcatcatgag aatatttttg
     2221 atatccagca ttggaccaat gacgagaaag gccagaactg gtgccaaacc gaaactcgag
     2281 agaagagaag cacctataaa ggcatccgcc tcactacaga gcgaaagaag aaaggccaaa
     2341 atcatcaaga gcaggatggc aaaaagagga gtcgcactga tagaggtcag aatccgagtc
     2401 ggaacgtaga cctgtattat agaagcaaag aggcagccaa ataccaaata acgccccgta
     2461 tcaaaaaatt catcaatggc ctgcacaaag acttgaaaaa ctttttttgc agaactcaag
     2521 taagaaaaat catgctcatg acaagccaga cgattttctt tctgaatcgg ttcttgccag
     2581 aaaaatccta gaaaaattcc tagtattaca gccacaagaa tggaacccag agctcgtaat
     2641 agggcgacat ggaaggagtt gccaaaggca gaataggtcg caaaaagaac aatgggattg
     2701 ataacaggtg ctgtcacaag aaaaggaacg gctgtgtaac tgggaacctt tttttccaga
     2761 aaacgattga tgatggggac gattccacat tcacaagaag ggaaaagcat accgacaaag
     2821 gtcccaaaaa agattctccc ccaacgattt cgagggagaa aatgataaac cttgtcaggt
     2881 gtgatataaa cttcaatcag ccctgagaca atgcttccta ttagaacgaa gggaagggct
     2941 tcaatgataa tggagagaaa aatggctcca gtttgaagta cactagatgg gagttgttga
     3001 aaagcagtca tctagttttt cttttctgct ttggcttttt tctctttgtc atcagggaaa
     3061 gtaacttgtt tcaaatcagg gagtttagca aactcttcaa acatcttgtc caagtcgtct
     3121 cgttttgtaa attttacagc cataggtgta cctcgattct aaagtctacc tctattatac
     3181 tattattttg cggaaaaggg agttttttaa tttgttttcg atagttgata cacttcgaaa
     3241 atctcttcaa accacgtcag cttcgcctta ccgtatatat gtgacttatc cacaacctca
     3301 aaacagtatt ttgagcagcc tgcggctagc ttcctagttt gctttttgat tttcattgag
     3361 tatgaaaagg attggagaaa gccttccagc ttgtggtata atagatttat ggataaaaaa
     3421 tatgaaaaaa tctctcagga tttgggagtg atgttaaagc aaattgatac cgttctaagt
     3481 ttgacagctg aaggggcgac tattcccttt atcgcgcgtt atcgcaagga catgactggt
     3541 agtctggatg aggtggcgat taaggctatt attgatttgg ataaaagtct gacaaatctc
     3601 aatgaccgta aggaagctgt cttagctaag attcaagaac aaggtaagtt gaccaaggaa
     3661 ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg ttgaagaact ctatcttcct
     3721 tataaggaaa agcgtcgtac caaggcaacc attgcccgtg aagctggact ctttcctctt
     3781 gctcgtttga ttttgcagaa tatagttgac ttagagaaag aagctgaaaa gttcgtctgt
     3841 gaaggatttg cgactggcaa ggaagccttg accggtgcag ttgatatttt ggtcgaagcc
     3901 ttatcggaag atgtgacctt gcgttctatg acttatcagg aagtgctgag acactctaaa
     3961 cttacttctc aagccaagga tgaaagtctt gatgaaaagc aggtttttca gatttattat
     4021 gatttttcag agacagttgg aactatgcaa ggctatcgta ccttggctct caatcgtggg
     4081 gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga cggaccgtat tcttgccttc
     4141 tttgctactc gtttcaaggt gaaaaatgct tatattgatg aagttgttca gcaatccgtt
     4201 aagaaaaagg tcttgcctgc tatcgagcgt cgtattcgga cagaattaac tgagaaagct
     4261 gaagaaggag ctatccaact tttttctgac aatctgcgca atctcctctt ggttgctcca
     4321 ctgaaagggc gcgtggttct tggatttgac ccagcctttc gtacaggtgc caagttagct
     4381 gtcgtggatg caacaggaaa aatgctgaca actcaggtta tttatcctgt taaaccagca
     4441 tcagctcgtc aaatcgaaga agccaagaaa gatttagcag atttaattgg tcaatacggt
     4501 gtagagatta ttgccattgg aaatggaacg gccagtcgtg aaagtgaagc ttttgtagcg
     4561 gaagttctga aagatttccc tgaagtcagc tatgttatcg ttaatgaaag tggtgcttct
     4621 gtctattctg ccagcgaact tgctcgtcag gagtttccag acttgaccgt tgaaaaacgc
     4681 tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg cggaattggt caaaatcgat
     4741 cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtca gtcagaagaa actatctgag
     4801 agtctggact ttgttgtcga tacagtggtt aaccaagttg gtgtcaatgt caatacagct
     4861 agcccagctc ttctttcaca cgtagctgga ctcaacaaaa ctatctctga aaatattgtc
     4921 aaataccgcg aggaagaagg aaaaatcact tcacgcgccc aaatcaagaa agttcctcgt
     4981 ctgggagcca aggcctttga gcaggctgct ggtttccttc gtatccctga aagtagcaat
     5041 atccttgata atacaggagt tcacccagag aattacactg cagttaagga actcttcaaa
     5101 cgtttagata ttaaagacct aaacgaagaa gcccaaagca aactcaagtc cctttcagtc
     5161 aaggaaatgg ctcaagagct agacctcggt ccagaaaccc ttaaagatat cattgcagat
     5221 cttctcaaac caggtcgaga tttccgtgat tcctttgacg cacctgtgct tcgccaagat
     5281 gtcctagata tcaaagacct agtggtaggt cagaagctag aaggtgttgt gcgtaacgtt
     5341 gttgacttcg gtgccttcgt tgatatcggg attcacgagg acggcttgat tcatatttct
     5401 catatgagtc gcaagtttat caaacaccct agtcaagtgg tgtcagttgg agatttggtg
     5461 accgtttggg tcaagaaaat tgatactgaa cgtgaaaaag ttaatctgtc gctcctcgct
     5521 ccaaatgaaa ctgactgatt atgtcaagca ggtttcacta gaagacttcg ggagagcttt
     5581 tatccatcat gtccagtgga ataggcgtct acgttcgaca ggtgggcgat ttttccccaa
     5641 agatgggcat ctggatttta atcccaaggt ttatcagaaa ctgggtatgg aggtttttag
     5701 gaaaattgtc cgacatgaac tctgtcatta tcacctttat ttccaaggga agggctatca
     5761 acacaaggat cgggatttta aggaactgtt gaaagcagtg gatggattaa ggtttgtacc
     5821 atccttgccc aatagtaact ctaaaccact caagctttat cgttgtcaat cctgccagca
     5881 aaggtatcag cgcaagcgta ggattgatac caaacgctat cgctgtggac tttgtcgagg
     5941 taaattgctt ctgataaatc agcctgagga ctgatgaaag ccgagcctcc ccgtgatata
     6001 ctatgtctaa cctagcagaa agaggaaata tcatgaacag taaattttat aaaatgagac
     6061 gcaatcgcat gatttcagga gttctggcag gtctgtcaga caagtggaat tttgatgtta
     6121 ccctagttcg tttcttgttt gccattttta cagttgcaaa ctttggactt ggcgtgatta
     6181 tctatatcat cctagcttct atcatgccta ccaaggaaga aatcgaagca gaaatgtacg
     6241 gaacaggccc acgtaaacgc aaagaagccc aagccattga cgacaatgaa ggctggtttt
     6301 ggtgaggttc tacagaagat ttggaataag caaaaagctt taaatcaata gtctaaactg
     6361 cttgatttaa agctttttta cttttatact caataaaaat caaagtgcaa actaggaagc
     6421 cagccacagg ctgtaattta gtcggcaatg tgaagttgac atagtttgca ctttgatttt
     6481 tgaatagtat taataatatt tcgccagctt catccacaaa ctatagtgga ttg
//
LOCUS       DAVBCR010000091         6508 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCR010000091 DAVBCR010000000
VERSION     DAVBCR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.091"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..211
                     /locus_tag="VQ508_001851"
     CDS             <1..211
                     /locus_tag="VQ508_001851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490806.1"
                     /translation="PNPSNRRDDRVELSHQVIDTFFRLVNEPLVMFDDFITRDKTSMR
                     YQTHYQLKQKAVVRSFRKSSSDWRK"
     gene            298..2064
                     /gene="patB"
                     /locus_tag="VQ508_001852"
     CDS             298..2064
                     /gene="patB"
                     /locus_tag="VQ508_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2490807.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2203..2892
                     /locus_tag="VQ508_001853"
     CDS             2203..2892
                     /locus_tag="VQ508_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2490808.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2896..3075
                     /locus_tag="VQ508_001854"
     CDS             2896..3075
                     /locus_tag="VQ508_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490809.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3062..4411
                     /locus_tag="VQ508_001855"
     CDS             3062..4411
                     /locus_tag="VQ508_001855"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2490810.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4535..5995
                     /locus_tag="VQ508_001856"
     CDS             4535..5995
                     /locus_tag="VQ508_001856"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2490811.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6009..6443
                     /locus_tag="VQ508_001857"
     CDS             6009..6443
                     /locus_tag="VQ508_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2490812.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2045 a         1250 c         1398 g         1815 t
ORIGIN      
        1 tcccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag tcattgacac
       61 atttttccgc cttgtcaatg agcctcttgt aatgtttgat gatttcatta cacgagataa
      121 aacgtctatg cgttatcaaa ctcattacca attaaaacaa aaagctgtgg ttagatcctt
      181 tcggaaatcg tcaagcgatt ggaggaaatg aactaatcca cagcggctta ttccaagtat
      241 accacttggg ctttggcagt agctaactgc gctaaatata atataaggag gagtaaaatg
      301 aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt tgtagttgtc
      361 atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt ttctggacaa
      421 gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc agaacttgta
      481 tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt tctatttatc
      541 tctagtgtaa tatacatgtg tctcatgacg cacgtgattg cagaatcgac caacgagatg
      601 cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga ccgtcgacaa
      661 gatggcgata tcttgtctca ttttaccagt gatttggata atatcctcca agcctttaac
      721 gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat tcttgtcatg
      781 ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt ggctttcctt
      841 atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca gaaagaggta
      901 gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa
      961 ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca
     1021 acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg gatgagcctg
     1081 attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga taagtctatt
     1141 gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca gcagtactac
     1201 cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt tactggagct
     1261 gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc
     1321 ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata cttgcctgat
     1381 aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac agcagttgtt
     1441 gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt ttatgatgtt
     1501 gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt agatagtctt
     1561 agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac gattcgagac
     1621 aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc agcaaaagca
     1681 acccacattc acgactatat cgaaagtttg cctgataagt acgatactct tattgatgat
     1741 gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg aaccctgatg
     1801 acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac ggtgacagaa
     1861 agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt cgtcattgcc
     1921 caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga tggagaagtc
     1981 attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc agaactctat
     2041 cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc tttttcttgt
     2101 ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt cattttgagt
     2161 gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag aacggttgta
     2221 ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca ttcatctgta
     2281 tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt cattcctgtt
     2341 ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat tttgacagga
     2401 ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag cgatgattac
     2461 aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg tcagaataaa
     2521 ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc
     2581 aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca ctctattgag
     2641 acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt caactcagtc
     2701 catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat tgattcaaga
     2761 gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg tttacagtgg
     2821 catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga gtatttattg
     2881 gatgaactgt aacaattgga acatctggtc gttctttctt ttattttttc aaaattttta
     2941 gaatgtggta ttttacgcaa acgtttgaat tctgataaaa attggagaaa ttcgacaaaa
     3001 aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt ggaggaagaa
     3061 catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg caccacatga
     3121 agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta aaggaactgg
     3181 tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc gcgaagaatt
     3241 tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt tggttgtgat
     3301 cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga atcaccattt
     3361 tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg caggaaactc
     3421 aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag acttctcagt
     3481 aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc gtgtctttaa
     3541 agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct atgcaacaac
     3601 tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg gaacatttgt
     3661 tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt tgcttccaat
     3721 cgcagcatca ggagctgaca taaaagctct tatggaaggt gcgaatgcag ctcgcaaaga
     3781 ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg ttcgtaacat
     3841 cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat cacttcaata
     3901 cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat
     3961 ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat ttatccaaga
     4021 aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct
     4081 tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa aagacgttga
     4141 ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc
     4201 aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca ctatctactt
     4261 cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat ttgaccaacc
     4321 aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga
     4381 attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt gcccactctt
     4441 tttactctct ttatccatag aaattggact cagccaagac ttgtgatata atatagaaag
     4501 caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc gcgtacgtta
     4561 cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat tgtttaatta
     4621 cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata ctgaccgtaa
     4681 acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt taggcatgga
     4741 ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt tggacttgta
     4801 tcaaaaatac atcgaccaac tattagctga aggaaaagcc tacaaatctt acgttacaga
     4861 agaagagttg gcagctgaac gtgaacgtca agaagtagct ggcgaaacac cacgctacat
     4921 caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag aacgtgaagc
     4981 agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct acaagtggca
     5041 tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg actgggttat
     5101 ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gttatcgatg accacgatat
     5161 gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa aacagcttat
     5221 ggtctatgaa gctcttggtt gggaagctcc agagttcggt cacatgacct tgattatcaa
     5281 ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat ttatcgaaga
     5341 ttaccgaaag aaaggttacc ttccagaagc agtctttaat tttattgctc ttcttggttg
     5401 gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac tttttgatga
     5461 aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact ggatgagcaa
     5521 tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac cattcttaga
     5581 ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata aaccacaaat
     5641 gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt tcccagaatt
     5701 gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt
     5761 caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata tcttcccaca
     5821 aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca tgcctattcg
     5881 tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt tcttgcttgg
     5941 acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta aataaagagg
     6001 atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta tacaatccac
     6061 atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa gcagaagatg
     6121 gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat ctctgcgcag
     6181 aacgggcggc cctcttcaat acgtaccaat tttcaggaca aactaaggtt aagaaagtat
     6241 tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc ggtgcttgta
     6301 gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg atggactata
     6361 atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga gaagaacgtg
     6421 cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt tactataagt
     6481 tgaaaaaata aaaaaaatta gaaaaagt
//
LOCUS       DAVBCR010000092         6476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCR010000092 DAVBCR010000000
VERSION     DAVBCR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.092"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            53..1015
                     /locus_tag="VQ508_001858"
     CDS             53..1015
                     /locus_tag="VQ508_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2490813.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1192..2199
                     /gene="gap"
                     /locus_tag="VQ508_001859"
     CDS             1192..2199
                     /gene="gap"
                     /locus_tag="VQ508_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2490814.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2266..3102)
                     /locus_tag="VQ508_001860"
     CDS             complement(2266..3102)
                     /locus_tag="VQ508_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2490815.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3228..5423
                     /locus_tag="VQ508_001861"
     CDS             3228..5423
                     /locus_tag="VQ508_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2490816.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ508_001862"
     CDS             5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ508_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2490817.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5638..5814
                     /gene="secE"
                     /locus_tag="VQ508_001863"
     CDS             5638..5814
                     /gene="secE"
                     /locus_tag="VQ508_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2490818.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5870..6406
                     /gene="nusG"
                     /locus_tag="VQ508_001864"
     CDS             5870..6406
                     /gene="nusG"
                     /locus_tag="VQ508_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2490819.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2012 a         1128 c         1418 g         1918 t
ORIGIN      
        1 cacggataca gttgggaaag aagctattaa acatttggag agtccttttg ggatgaacat
       61 gtcaaatatt actatagatg ggcaagaaat ggatctgagt caaacgatgc cttcttatgc
      121 tcaagtagtg agtctttatg atgatacaag tatctctgtt agtagtaatg agacggacaa
      181 agtgttggct ggctccctct atactgatac aaatgaacag ggattaacga ttccaagcag
      241 tttactaaaa aattggaatg aacagacagg aaaaaatttg acagctaatg atcttattgg
      301 caaatcagtc tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt
      361 tcaaacgaag attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt
      421 catgctgtct catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc
      481 ttattttatc ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca
      541 gaaaaataag aagtatactg tgctttctca acagagagtt cttgatattg tgattatctt
      601 tattcgtgtt attcagggat tattgattgt gctttcatca caagctattg tggtagcagc
      661 ggttatgatt ggtatcatta tttacatcaa tatcatgcag cgttccaagg aaataggtgt
      721 catgaaggca gttggttatc agaatcgtga tgtcaaagga atttttattt acgaggctat
      781 ctggattgta ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt
      841 ggcgaatgcg attgtaagtc acttttaccc atccatcact aaagtttttg aattaaatct
      901 tttatctgtt ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt
      961 cccggcgcgt aagattagta aaatggatcc tgtagaaccg ttacgctatg aatagtaagg
     1021 gattccttga tttacagtct taaaaagaaa ctaacataga aaaacaatgg acatacttgc
     1081 aattcaactc aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt
     1141 tactcttttc acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt
     1201 aaagttggta ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac
     1261 gtagaaggtg ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac
     1321 ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt
     1381 ggatttgaag ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc
     1441 gactgggcta ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa
     1501 gaagcagctg aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt
     1561 ggaaacgacg ttaaaacagt tgtattcaat actaaccacg acgttcttga cggtactgaa
     1621 acagttatct caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt
     1681 caagacaact ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac
     1741 caaatgatcc ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct
     1801 gcaaacatcg ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa
     1861 ttgaatggta aacttgatgg atctgcacaa cgcgttccaa ctccaactgg atcagttact
     1921 gaattggtag cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa
     1981 gcagcttcaa acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta
     2041 ggtatgtctt acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt
     2101 aaacaattgg ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt
     2161 gttcgtactc ttgaatactt cgcaaaaatt gctaaataat tcttgagttg atagaaagca
     2221 aggctttgtg gtcttgcttt ttatatggaa aaatggatga cacgatcatc cattcttttt
     2281 taattctttt tcaaatgtat ttgaaagggt agtgaaagtt agcttctcta aagtaagtgg
     2341 gtgggtaaag gaaagtcgga aggcatgaag cataagccga cttgtctttg atttactatt
     2401 atagagaggg tcgcccagga taggaagatt atgatgcgaa aggtgcacac gaatctgatg
     2461 ggttcgccct gtctttagct tgcaacgagc caaggaagtc ttgtttgaga attgctttaa
     2521 tctgcttaca tacgtttcag catattgccc attttttgca tcaactattc tttttctacg
     2581 atcatggcga tcacgtccaa ttttgtctct gaaaacaagt tcttttctgt tgatatttcc
     2641 atcaactaga gcccaatatt ctctagaaat ctcttttttc tccaataagc gattgagaat
     2701 gggcaggata aaaggatttt tggcaaagag aactaagcca ctggtttcca tgtccagacg
     2761 atgaacgaca tagcaggttt ggccaacata ggtactgaca tggttaagaa gggcaatttc
     2821 gtttggttga ttaccatgcg ttttcatccc ctctggtttg tttacaataa tcaagtattg
     2881 atcttgataa acttcctgca ctaagtctgg gttgccccaa gggatcgtct tttggggata
     2941 atcttcctcg tcaaaagtca actggcaaac atctccagga tttacgattt cgttccagtg
     3001 gacttcttct tgatttatca aaatatgttt cttgattctc aaaaaatgac ggatttttct
     3061 agggatgagg agttgttcct caagtaattg ctttaccgtc atttgaggta gagaggcggg
     3121 taatgtaaat gtgaattgca tacagatatt gtaacaaaaa aagccctatt tggataggaa
     3181 atagctaaat tcttgtcttc ctatgatgaa gatgataaaa taaacgcatg aaattagata
     3241 aattatttga gaaatttctt tctcttttta aaaaagaaac aagtgaacta gaggactctg
     3301 attctactat cttacgtcgc tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga
     3361 ttcgaaaatt ctggcgtcgt tatcatctaa caaagattat ccttatacta ggtttgagtg
     3421 caggcttgct agttggaatc tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt
     3481 tgcaaaatgc cttgaaaact cggactctta tttttgaccg tgaagaaaaa gaggctggtg
     3541 ccttgtctgg tcaaaaggga acctatgttg agctgactga tatcagtaaa aacttgcaga
     3601 atgctgttat tgcgacagaa gaccgttctt tctataaaaa tgacgggatt aactatggcc
     3661 gtttcttctt ggctattgtc actgctggac gttcaggtgg tggctctacc attacccaac
     3721 agctggctaa aaacgcctat ttatcacagg atcaaactgt tgagagaaaa gcgaaagaat
     3781 ttttccttgc cttagaatta agcaaaaaat atagtaagga gcaaattcta accatgtacc
     3841 ttaacaacgc ttattttgga aatggtgtgt ggggtgtaga agatgcgagt aagaaatact
     3901 ttggagtttc tgcatcagaa gtgagtctgg atcaagctgc gactctggca gggatgctca
     3961 aggggccgga actgtataat cccttgaatt ccgtagaaga ttctactaat cggcgcgata
     4021 ctgtcttgca gaatatggtt gcagcaggat atattgataa aaaccaagaa accgaagctg
     4081 ctgaagttga tatggcttcg caattgcacg ataagtatga aggaaaaatc tcagattacc
     4141 gttacccctc ttattttgat gcggtggtta atgaagctgt ttccaagtat aatctaacag
     4201 aggaagagat tgtcaataat ggctaccgca tttacacaga gctggaccaa aactaccaag
     4261 caaatatgca gattgtttat gaaaacacat cgctatttcc gagggcagag gatggaacgt
     4321 ttgctcaatc aggaagtgta gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg
     4381 gtcaagttgc tgacaatgat aaaactggat tccggaattt caactatgca acccaatcaa
     4441 agcgtagtcc tggttctaca attaagcctt tagttgttta tacgccagca gttgaagcag
     4501 gctgggcttt gaataagcag ttggataacc ataccatgca gtatgacagc tataaggttg
     4561 ataactatgc agggatcaaa acgagtcgag aagttcctat gtatcaagcc ttggcagaat
     4621 cgcttaatct acctgctgtt gccgctgtta atgatttggg tgtcgacaag gcttttgagg
     4681 caggcgaaaa attcggactc aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg
     4741 gaagcggtgt tgaaaccaac cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg
     4801 aaggtttaat gcctgaagct cattttatta gtagaattga aaatgctagt ggacaagtta
     4861 ttgcgagtca taaaaattca caaaaacggg tgattgataa gtctgtagct gacaagatga
     4921 ccagtatgat gttggggact ttcaccaacg gtaccggtat tagttcatcg cctgcagact
     4981 atattatggc agggaaaact ggaacaactg aagcagtttt caatccggag tacacaagtg
     5041 accagtgggt aattgcttat actccggatg tagtgattag ccactggctt ggctttccta
     5101 ccactgatga aaatcactat ctagctggct ctacttcaaa cggtgcagct catgtcttta
     5161 gaaacattgc caataccatt ttaccttata cgccaggaag tacctttacg gttgaaaatg
     5221 cttataagca aaatggaatt gcaccagcca atacaaaaag acaagtacaa accaatgata
     5281 atagccagac agatgataat ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg
     5341 aggctagccg ggctatctca gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt
     5401 cgatagtcaa tctatttcgc taagatgctt gtcaaagcct agctttcttg ttataatgga
     5461 taggatggag gcgttatggc actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg
     5521 agaaactatt caatcaagat cagcggaaac cccaagccta cacgactaga agtaaataaa
     5581 ttttgtaagc attgtggcaa gtacactaca cacagagaaa cgagatagga gagagcgatg
     5641 cgttttattg gagatatttt tagacttctt aaagacacaa cgtggccaac tcgcaaggaa
     5701 agctggagag attttcgttc tatcatggaa tacacagctt tctttgtagt aattatttac
     5761 atttttgacc agttgattgt ttcaggtttg attcgattta ttaacatttt ttagaagatt
     5821 agtggagtta attacactag aaatcttcta tattatgaaa ggaaatatca tggatagttt
     5881 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
     5941 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
     6001 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
     6061 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     6121 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     6181 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     6241 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     6301 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     6361 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct
     6421 ggagcctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatg
//
LOCUS       DAVBCR010000093         6434 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCR010000093 DAVBCR010000000
VERSION     DAVBCR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.093"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            209..409
                     /locus_tag="VQ508_001865"
     CDS             209..409
                     /locus_tag="VQ508_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490820.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            434..850
                     /locus_tag="VQ508_001866"
     CDS             434..850
                     /locus_tag="VQ508_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490821.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ508_001867"
     CDS             1081..1788
                     /gene="ccdA2"
                     /locus_tag="VQ508_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2490822.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ508_001868"
     CDS             1799..2356
                     /gene="sdbB"
                     /locus_tag="VQ508_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2490823.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2477..3868
                     /locus_tag="VQ508_001869"
     CDS             2477..3868
                     /locus_tag="VQ508_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2490824.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ508_001870"
     CDS             4011..4928
                     /gene="adcAII"
                     /locus_tag="VQ508_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2490825.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4936..>6434
                     /locus_tag="VQ508_001871"
     CDS             4936..>6434
                     /locus_tag="VQ508_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2490826.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1975 a         1170 c         1351 g         1938 t
ORIGIN      
        1 tttctcttac caacattaac cacaccatcg gttattatag taaaatggaa taagaacagg
       61 acaaatcgat caggacagta aaatcgaatt ctaacaatgt tttagaagtc tatatgtact
      121 attctagttt caatatacca taactgctcc tagtcaacct gcaagtcctg tcccagctcc
      181 aagctcagct ccgactcaag atcctagagt ggtagcaagc gcttcggcta gctcaacgag
      241 cacacaagct caagagcaag ttgacaagtc tgaacttcgt gccttgagtc aagagttaga
      301 ccaacgcttg aaagctttgg caacagtatc tgatccaaaa atcgatgcaa ccaaggctgt
      361 cctcctagat gctcaaaaag ctccggaaga tagtgccttg acagagtaag gtctgcatac
      421 tgcagtagag tctgtgaagg ctgccttgga ctctctcaaa gatgtgaaag caaatgctag
      481 cgacagcaag cctgcacagg acaagaagga tgcaaaacaa ggaacggaag atagtaagga
      541 ttcagataag atgactgaaa caaactcagt tccggcagga gtgattgtgg tcagtctact
      601 tgccctccta ggcgtgattg ccttctggct gattcgccgt aagaaagagt cagaaatcca
      661 gcaattaagc acggaattga tcaaggttct aggacagcta gatgcagaaa aagcggataa
      721 aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc cttgatttcg tgaaagaaga
      781 aaatggctca gcagagacag aaactaaact agtagaggag cttaaagcaa tccttgacaa
      841 actcaagtaa aacatgattt atgaaatgtc agacctaaaa gacattttcg ttcacaaaag
      901 cagtgacgag taatccttgt cgttgctttt tggctatttt gggggataag atccgccttg
      961 actagcaaaa gaaaagattg gtccaatagt gcccgatacg attttgcgta gcagaaagct
     1021 tacaaataaa attgaatata gtaaaatagg attagaatta ttaagaaagt tggttcttta
     1081 ttggaaacga tagtattttt aatctctgtt tttctagcag gtgttttatc ctttttttct
     1141 ccttgtattt ttcctcttct gccagtctat gctgggattt tattggatga tcaagaaagt
     1201 gcaaaaagct tttctttgtt tgggagaaag gttctctggt caggcttgat tcgaacactt
     1261 tgctttatcg ctggtatctc tctcattttc tttattctag gttttggtgc tggttacttt
     1321 ggtcatattc tctatgcaaa ttggtttcga tatgtcatgg gagctattat tatcattttg
     1381 ggtcttcacc agatggaaat ttttcatttg aagaaattag aagttcaaaa aagttttacc
     1441 tttaaaaaat cagattctaa tcgttattgg tcagcttttt tacttggtat tacctttagc
     1501 tttggttgga caccttgtat tggtccagtt ttaagttctg ttttagcact tgcggcttct
     1561 ggaggcaatg gtgcttggca aggagcgatt tatactctca tttacactct gggcatggcc
     1621 cttcctttct tggtcttggc actagcttca ggtctagtca tgccatattt tagtaaaatc
     1681 aagcgtcata tgatgctact aaagaaaatt ggtggtttcc tcattgtttt aatgggaatt
     1741 ttgttactat taggacaagt aaatgttcta gctggaattt ttgaataaag gaggacaaat
     1801 gaaaaaagta atgtttgctg gcttaagtct cttgtcatta gttgtattga tggcctgtgg
     1861 tgaggaagaa actaaaaaga ctcaagcagc acaacagcca aaacaacaaa cgactgtaca
     1921 acaaattgct gttggaaaag atgctccaga cttcacattg caatccatgg atggcaaaga
     1981 agttaagtta tctgatttta agggtaaaaa ggtttacttg aagttttggg cttcatggtg
     2041 tggtccatgc aagaaaagta tgccagagtt gatggaacta gcggcgaaac cagatcgtga
     2101 tttcgaaatt cttactgtca ttgcaccagg aattcaaggt gaaaaaactg ttgagcaatt
     2161 cccacaatgg ttccaggaac aaggatataa ggatatccca gttctttatg ataccaaagc
     2221 aaccaccttc caagcttatc aaattcgaag cattcctaca gaatatttaa ttgatagcca
     2281 aggaaagatt ggaaagattc aatttggtgc tatcagtaat gcggatgcag aagcagcatt
     2341 taaagaaatg aactagaatc gataaaatct gattacaaga tgtagtcaga tttttttaga
     2401 aaaacatttt ataaatattc acatatctcc tatatttatg gtaaaataga attatcagtt
     2461 tattttggag tcaaagatga atatatttag aacaaagaat gttagtttag ataaaacaga
     2521 gatgcatagg catttgaagt tatgggattt gattttgctg ggtatcggag ccatggtagg
     2581 gacaggcgtc tttacaatca caggtactgc agctgcaaca cttgctggcc cagccctagt
     2641 gatttcaatc gttatttctg ccttgtgtgt gggattatca gccctctttt ttgcagaatt
     2701 tgcctcgcga gtacccgcta caggaggtgc ctatagttac ctctatgcta tcttaggaga
     2761 attcccttcc tggttggctg gttggttaac catgatggag ttcatgacag ccatatcagg
     2821 cgtagcttcg ggttgggcag cttattttaa aggcttgctc tctcaatacg ggatagcctt
     2881 tcctcaggct ttaaatggta cctttaatcc tcaagcaggg acatttgttg atttattgcc
     2941 tattcttgtc ttggtcttgg tgacttcgct tgttttacta aatgccaagg cagccttacg
     3001 ctttaattcg attctagtta ttttgaaatt ttccgcttta gctctctttg ttttagtagg
     3061 aatttggaat attaagtttg ataattggag caattttgct ccttacggtt ttggacaaat
     3121 ctatggagct agcactggta ttatggctgg tgcgtccttg atgttcttcg gttttttggg
     3181 atttgaatcc atctctatgg ctgtagatga agtcaagact cctcaaaaaa atattcctag
     3241 agggattgtc ttatcgcttt ctatcgtaac cattctctat gccttggtga cccttgtttt
     3301 gactggtgtg gttcactata gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg
     3361 tagtgttggg attagttggg cagccaacta tgtgtcatta gtggctatct tgaccttgat
     3421 tacagtttgt atttcgatga cctatgccct atcgcgtatg atttacagtt tagcacgtga
     3481 cggcttagtg cctgctgcct ttaaggaact gacgaagact agcaagatac caaagaatgc
     3541 tactatcttg acaggtctag cttcagcagt agcagcagga atgttcccac tagccagtat
     3601 tgcagccttt ttaaatattt gtaccttagc ctacttgatc atgctggctt atggtctgat
     3661 tcgcttacgg aaagaaaaag gaatgcccaa agctggggaa tttaaaacac cattggtacc
     3721 tctgttacca attttatcga tcatcatttg tttatccttc atgttacagt ataatatgaa
     3781 cacctggatt gctttcctag ttgcgttgtt agtaggaagt attatttact tcacttatgg
     3841 ctataagcat tctaccatag aagaataaag tgaaagtcta ttgagttgtt gacactcagt
     3901 agatttttat atgtgaaaga aatttcaaat tttttctaaa aatatcttga cagattaaat
     3961 tttcaggagt agaatattta ctagttaatt aaatggttaa ggagttgttc atgaagaaac
     4021 aaaatttatt tttagtcctg ttaagtgtct ttcttttgtg tttgggggct tgtggtcaaa
     4081 aggaaagtca gacaggaaag gggatgaaaa ttgtgaccag tttttatcct atctacgcta
     4141 tggttaagga agtatctggt gacttgaatg atgttcggat gattcagtca agtagtggga
     4201 ttcactcctt tgaaccttcg gcaaatgata tcgcagccat ctatgatgca gatgtctttg
     4261 tttaccattc tcatacactc gaatcttggg caggaagtct ggatccaaat ctaaaaaaat
     4321 ccaaagtgaa ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc cctggactag
     4381 aggatgtgga agcaggggat ggagttgatg aaaaaacgct ctatgaccct cacacatggc
     4441 tagatcctga aaaagctgga gaagaagccc aaattatcgc tgataaactt tcagaggtgg
     4501 atagtgagca taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg
     4561 aattgactaa gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac
     4621 aacatacagc cttttcttat ctagcgaaga gatttgggct taatcaactt ggtattgcag
     4681 gtatctctcc tgaacaagaa ccaagtccac gacaactaac agaaattcag gaatttgtta
     4741 agacctataa ggttaaaacg atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa
     4801 ctcttgtcaa atcaacaggt gtgggtctta aaactctgaa tcctttagag tcagacccac
     4861 aaaatgacaa gacctattta gaaaatcttg aagaaaatat gagtattcta gcagaagaat
     4921 taaagtgagg aaagaatgaa aatcaataaa aaatatctag caggttcagt ggcagtcctt
     4981 gccctaagtg tttgttccta tgaacttggt cgtcaccaag ctggtcaggt taagaaagag
     5041 tctaatcgag tttcttatat agatggtgat caggctggtc aaaaggcaga aaatttgaca
     5101 ccagatgaag tcagtaagag agaggggatc aacgccgaac aaattgttat caagattacg
     5161 gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg caaggttcct
     5221 tatgatgcca tcatcagtga agaacttctc atgaaagatc cgaattatca gttgaaggat
     5281 tcagacattg tcaatgaaat caagggtggc tatgtgatta aggtagacgg aaaatactat
     5341 gtttacctta aagatgcagc ccatgcggac aatattcgga caaaagaaga gattaaacgt
     5401 cagaagcagg aacgcagtca taatcacaac tcaagagcag ataatgctgt tgctgcagcc
     5461 agagcccaag gacgttatac aacggatgat gggtatatct tcaatgcatc tgatatcatt
     5521 gaggacacgg gtgatgctta tattgtccct cacggcaatc acttccacta tattcctaag
     5581 agtgatttgt ctgccagtga attagctgct gcccaagcct attggaatgg gaagcaggga
     5641 tctcgtcctt cttcaagttc tagtcataat gcaaatccag ctcaaccaag attgtcagag
     5701 aaccacaatc tgactgtcac tccaacttat catcaaaatc aaggggaaaa catttcaagc
     5761 cttttacgtg aattgtatgc taaaccctta tcagaacgcc atgtagaatc tgatggccta
     5821 gtatttgatc cggcacaaat aacatatcgt acagtcaatg gtgttgctgt accgcacgga
     5881 gaccattatc actttattcc ttattcacaa ctgtcacctt tggaagaaaa attggctcgt
     5941 atgattgctg ttaagggaca aaatggtgca gtcttgccag gaatgcatta tttgaaacca
     6001 gcaccaaaac cgcaagttca accatcaact gagaaaaaac aaactgattt tgcagtagaa
     6061 caggttgtta gaaaagttgg agaaggctat gttgtggaaa ttgctggtgt cagtcactat
     6121 gtttttgcta aagacttggc taaggataag attgatgcta ttgaaaatct cttgtctaag
     6181 aaaactcaag agacacatgc tctagttgct aaaaaagaaa atgtcgctcc tcgtgaccaa
     6241 gaattttatg ataaagctta caatctgtta acccaggctc ataaagtctt gtctgaaaat
     6301 aaaggtcgta cttctgattt ccaagcctta gacaaattag cagaacgctt gaataatgaa
     6361 tcttctaata aagtaaaatt ggtagatgat ttattggcat tcctagcacc aattacccac
     6421 ccagaacgcc ttgg
//
LOCUS       DAVBCR010000094         6111 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCR010000094 DAVBCR010000000
VERSION     DAVBCR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.094"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..69)
                     /locus_tag="VQ508_001872"
     CDS             complement(<1..69)
                     /locus_tag="VQ508_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490827.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEK"
     gene            283..2052
                     /locus_tag="VQ508_001873"
     CDS             283..2052
                     /locus_tag="VQ508_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET2490828.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2076..4031
                     /locus_tag="VQ508_001874"
     CDS             2076..4031
                     /locus_tag="VQ508_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2490829.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTIKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4033..4470
                     /locus_tag="VQ508_001875"
     CDS             4033..4470
                     /locus_tag="VQ508_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2490830.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4532..5668
                     /locus_tag="VQ508_001876"
     CDS             4532..5668
                     /locus_tag="VQ508_001876"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET2490831.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
BASE COUNT         1865 a         1029 c         1238 g         1979 t
ORIGIN      
        1 cttctctaag ctatatcctt gttttctaag ttcatagatc tgaactttat catcataagt
       61 taacttcatg ataaaaacac cccaaaagtt agattttttc tgtctaactt ttggggtgca
      121 gttcatcatt tcaacatcct atactatgcg ttttataatt tttaagcctt tttgcccagc
      181 ctcgtcaaaa gtaatgtttt gacacaaaat ctgtgacaaa actttagttt taaaggtttt
      241 taactttgta tatactagtt ttaagaaaag gaggatgatc taatggaaga aaaagtatca
      301 ttgaaagtca gggttcaaaa actagggaca tcgctttcaa atatggttat gcccaatatt
      361 ggagcattta ttgcttgggg agtattgact gccctcttta tcgctgatgg ctatctgcca
      421 aatgaacagt tagctactgt tgttggtcct atgttaacgt atttattgcc aatcctgatt
      481 ggttacacag gtggatatat gatccatggc caacgtggtg ccgttgtagg agctattgct
      541 actgttggtg caatcacagg ttctagtgtt cctatgttta tcggagctat ggtaatgggc
      601 ccactgggag gatggactat caagaaattt gatgagaagt tccaggaaaa aattcgtccc
      661 ggatttgaaa tgttagttaa taacttctca gctggtctcg ttggttttgc attattgctt
      721 ttggctttct acgcaatcgg tccagtcgta tcgactctta ctggagctgt tgggaatggt
      781 gttgaggcta ttgtcaatgc tcgcctcctt cctatggcta atattatcat cgaaccggct
      841 aaagtccttt tcctcaataa tgccctcaat catggcattt ttactcctct gggagtagaa
      901 caggtagctc aagctggtaa gtcaattctc ttcctattgg aagctaatcc tggaccaggc
      961 ctgggaattc tattagctta tgctgtattc ggtaaaggtt ctgctaaatc ttcttcttgg
     1021 ggggcaatgg ttattcattt cttcggaggg attcatgaaa tttactttcc ttatgttatg
     1081 atgaagccta ctctattttt agctgctatg gcaggaggta tctctggaac ttttactttt
     1141 caactcttag acgctggtct taaatctcca gcttcaccag gttctattat tgcgattatg
     1201 gctacggcgc caaaaggtgt ttggcctcat ctaaatgttc ttttaggtgt tttagtggca
     1261 gcagttgttt ctttccttgt agcagccctt attcttcatg cagacaagtc aactgaggat
     1321 tcgctcgaag ctgctcaggc ggctacccaa gcagctaagg ctcagtctaa aggtcagtta
     1381 gtatcaactt ctgttgatgc agttgtttcg acagactcag tggaaaaaat cattttcgcc
     1441 tgcgatgctg gtatgggaag ctctgctatg ggagctagta ttcttcgaga taaggttaaa
     1501 aaagcaggtc tagagattcc agtatctaat caggcaatct caaatttgct tgatacacca
     1561 aaaacattaa ttgttactca ggaagaactg acaccaagag ctaaagacaa gagtccaagt
     1621 gctattcatg tttctgttga taatttctta gcgtcccctc gttatgatga aattgtagct
     1681 tcattaacag gagcttttcc aatagcagaa attgaaggag atataccaac ttcagcacca
     1741 gtagatagtc aggaaattga ccttaaccat attgatgctg tagtagttgc ttatggtaaa
     1801 gcacagggaa ctgcaactat gggctgtgaa acgattcggg ctatctttag aaacaagaat
     1861 attcgtattc cagtttctac tgccaaaatt tcagaattag gtgaatttaa ttctaaaaac
     1921 ataatgattg taacaactat ttctttacag gcagaagtgc agcaagcagc accgaattct
     1981 caatttctta ttgtggatag tttagtaaca acaccagaat atgacaaaat ggctgctaga
     2041 atgtacaaat agaactagag gtttctaaat tacgaatgct attaaccaaa cgagaagaac
     2101 aattattgaa ggctttccta catgtaggga agctttcaat gcaagatatg actgaaatct
     2161 tacaggtttc atctagaaca atttatcgaa ctttatcaga tttgacagat agcatggagc
     2221 aatatggaat cgaaataacg aagcatggga aatactatat tttgactgga gagttggatg
     2281 atttgccgac agaacttgaa gtgttagttg agtatagtcc ccaagaaaga caagagttga
     2341 ttacctatcg ccttctgact gagagcggtt ttgtcaccaa tgaagcattg caagagtgca
     2401 cgaaagtcag taatgtaact attattcagg atatttcaga tattgataag cgtcttttag
     2461 actttgatct gaaaattgaa cgacaaaaag gttatcggat ttctggtgat tcagttggta
     2521 agagaagatt tttggctatt ttactgacaa actgtatctc agtagcagat ttttcaaccg
     2581 gtaattttgg gagctttgat attttagaag cagatagaac taggctggcc agtcagattg
     2641 ttaataagca actgtcaggt tttccagata tggatgctag gatgaagatg ttttttgcga
     2701 tcttgttatc tcttataggt caggagcaaa acattgaaaa ttcacctaat actagtaagc
     2761 aggctttgga aatttctcaa aaaatttttc aaacttactc taagcagact gcacaatttt
     2821 atagtattca ggaaattatc tattttgcga gcatcttgga tgaattaatc attaaacgtc
     2881 aggacaatcc gctctttacg gagaaatttg atggtgaatt tttctacaat atttcaaatc
     2941 tgattgatac ggtttccatg tataccaaga ttgacttttt taaggacaag gttttattca
     3001 attttctttt ccatcatatt cggctcagtt taggcgtccc tatccttttt cagggtgaaa
     3061 atttgccaga atctgtccag attttagttg aaaggaataa atttctttat acagtcatca
     3121 gtcttttagt gaatgatatt tttccgaaat atcttcatac agagtatgag tatggcatga
     3181 ttgccctaca ttttatctct agcttaggcc gtagtccaga gatttatcca gtccgtgttt
     3241 tgcttttaac ggatgaacgt cgggtcacta gagatttatt agtcagtaaa attaagagtg
     3301 ttgctccttt tgtagagttg atagatattc agtctctagt agattaccac agtattgatc
     3361 tcagtcagta tgattatatt ttatctacca agccgctgac taatcaggaa atcgatgtaa
     3421 tttctagttt tccaaccatc aaagaattgc ttgaattaca ggaacgactt cagtatgtac
     3481 aggcacatcg tacaattgtc gcgcgtgatg ctatcgctcc agagaaaagt tatgacttgc
     3541 aagattattt aatatctagt agtcagcttt tgagtcaatt cgagttggtt caattggaga
     3601 ataatcaatc atttgagcac acggtagaac aaatcatcca atatcagaag aatgtgagtg
     3661 acagagatta cctaacaaga aaattgttat ctcacttcca gaatagtcct atggctattc
     3721 ctaatactgg tctggtgctt ttacatagtc agtctagcaa agtaacaaca aatagtttta
     3781 ctatgtttga actcaaacta cctatctccg cattgtcaat gaaacgagag gaagaagagg
     3841 tcaaaaggtg tctgctaatg ctaatgtcta aagaagctag cgaggaagcg agagatttaa
     3901 tgacagctat tagtcagtcg attattgaaa atcatcttta tacagagatt tacaagacgg
     3961 gaaatcaatc cattatttat cagatgctaa atactatttt taacgaaaaa attaagaaat
     4021 tggagaacta atatgaaact tgaaaaacat ttgattaagc ttaataaaca attttctaac
     4081 aaggaggaag ctatttgtta ttgtgggcaa gttctttatg agggtggata tgttaatgaa
     4141 gactatattg aagccatgat tgagcgagat aaagagctat ctgtttacat gggtaacttt
     4201 atcgccatac cgcatggaac agatgcagca aaaaaggatg tcctcaagtc tggtattaca
     4261 gtcgttcaag tccctagagg ggttgatttt gggaatgtat ctaaccctca agtggcaacg
     4321 gttctttttg gtattgctgg tattggtaat gaacacttag aaattattca gaaaatttct
     4381 atcttctgtg cagatgtaga taatgttctt aaactagccg atgctcagtc aaaagaggaa
     4441 gtattgcgct tatttgatgc tgttgaataa ttgaatttcg tcatttgcca tctagtatat
     4501 atgtccctca aataggaaaa ggagaaattg aatgaaacat tctgttcatt ttggtgccgg
     4561 taatatcggt cgtggtttta taggtgaaat tctatttaaa aatggtttcc atattgattt
     4621 tgtggatgtc aataatcaga taattcatgc tctaaatgaa aagggcaagt atgaaattga
     4681 aattgcacag aaaggacagt ctcgtataga agtaactaat gtggctggca ttaatagcaa
     4741 agaacatcct gagcaagtca ttgaagcgat tcaaaagacg gatattatta ctactgcaat
     4801 cggacctaat atactccctt ttatcgctga acttctagcc aaaggaatcg aagctcgccg
     4861 agttgcaggg aatacacagg tattggatgt tatggcctgt gaaaatatga ttggcgggtc
     4921 tcaatttctt tatcaagaag tcaagaaata tttaagtccg gaaggtttga catttgctga
     4981 taactacata ggttttccaa atgctgcagt agacaggatt gttccagcac aaagtcacga
     5041 agattccctt tttgttgtgg tcgagccctt taatgaatgg gtcgtggaaa ccaagcgtct
     5101 taaaaatcca gatttacgtc taaaagatgt gcattatgaa gaagatttag aaccctttat
     5161 tgagcgaaaa cttttttcag tcaattctgg acatgcaact tcagcttaca ttggtgcgca
     5221 ttatggtgcc aagacaattt tggaagctct tcaaaatcct aatatcaaat ctcggattga
     5281 atctgtatta gctgaaattc ggagtctctt gattgccaaa tggaactttg ataaaaaaga
     5341 attggagaat tatcacaaag tcattataga acggtttgaa aatcctttca tagtggacga
     5401 ggttagtcgc gtagctcgta ctccaatccg aaaattaggc tataatgaac gattcatccg
     5461 gccgatacgt gaattgaaag aactcagttt gtcatataaa aacctactta aaacagttgg
     5521 ctatgccttt gactatcgcg atgtaaatga tgaagaaagt attcgattag gtgaattgtt
     5581 ggctaaacaa tcagtcaaag atgttgttat acaagttaca ggtttagacg accaagaatt
     5641 gattgatcaa attgtagagt atatttaata tttttcgaaa atctcttcaa atcaggttag
     5701 catcgctttg tcttaggcat atgttactga ctccgtcagt cttatctgca acctcaaaac
     5761 actgtttgag ttatctgcgg taatctttct agcttgtctt tgatttttgt tgttatttat
     5821 aaggtaaaag aagctggaca aaaagtcttc aaaatcggaa aaaggcagcc tatcgggtgt
     5881 tcaaaaatct tgataggatg tcctttatta tggaaagcct tattggattt tctcctcaga
     5941 ttgagttttt gatcagcttt atgagatagg ttttgctaga gatgtagccc atcatgctat
     6001 ttttatggac agtgggaaaa ttgttgaaaa aaataatgcc catcaattct ttagtcgtcc
     6061 aagagaagaa cgaaccaagc aattttggaa cgaattcttt cgaatgcgat c
//
LOCUS       DAVBCR010000095         6040 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCR010000095 DAVBCR010000000
VERSION     DAVBCR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.095"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            226..1392
                     /locus_tag="VQ508_001877"
     CDS             226..1392
                     /locus_tag="VQ508_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2490832.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1508..2545
                     /locus_tag="VQ508_001878"
     CDS             1508..2545
                     /locus_tag="VQ508_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2490833.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2753..3607
                     /locus_tag="VQ508_001879"
     CDS             2753..3607
                     /locus_tag="VQ508_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2490834.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            3757..4014
                     /locus_tag="VQ508_001880"
     CDS             3757..4014
                     /locus_tag="VQ508_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2490835.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(4390..4482)
                     /locus_tag="VQ508_001881"
                     /pseudo
     CDS             complement(4390..4482)
                     /locus_tag="VQ508_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4518..4814)
                     /locus_tag="VQ508_001882"
     CDS             complement(4518..4814)
                     /locus_tag="VQ508_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2490836.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(4804..4965)
                     /locus_tag="VQ508_001883"
     CDS             complement(4804..4965)
                     /locus_tag="VQ508_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2490837.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            5035..5799
                     /locus_tag="VQ508_001884"
     CDS             5035..5799
                     /locus_tag="VQ508_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2490838.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
BASE COUNT         1706 a         1122 c         1403 g         1809 t
ORIGIN      
        1 ttattcggca acctcaaaac ggtgttttga gctgacttcg tcagtcttat ccggcaacct
       61 caaagcagtg ctttgagcag cctgcggcta gtttcctaca gattttagtt ggaactcgat
      121 tcaattcatg tgacaacgtg aaaatcgtta gagcatttta tatagaatat acatgggaat
      181 gtagcttact cccattccca tatttaatag aaaaagagga actcaatgct acattataca
      241 aaagaagact tgctcgaatt gggtgcagaa atcactacgc gtgaaatcta ccaacagcct
      301 gatgtatgga gagaagcttt tgaattttat caagcaaaac gtgaagaaat tgcagccttc
      361 ctacaagaaa tcgctgataa acatgactat attaaggtta tcttgacagg tgctgggact
      421 tctgcttatg tgggagatac cttgctacct tattttaagg aagtctatga cgaacgcaaa
      481 tggaatttca atgctattgc gacaacagat atcgttgcca atccagcaac ctatttgaaa
      541 aaagatgtgg caactgtcct tgtgtctttt gctcgtagtg ggaattcgcc tgaaagtttg
      601 gcgactgttg atttggccaa atccttggtg gatgagcttt atcaagtgac gattacttgt
      661 gcagcagatg gtaaattggc tcttcaagct cacggtgatg atcgtaatct cttgctcttg
      721 caaccagctg tctctaatga tgctggattt gccatgactt ctagctttac gtctatgatg
      781 ttgacaactc tcttggtctt tgatcctaca gaatttgctg ttaagtctga acgttttgaa
      841 gttgtatcta gtcttgcccg taaagtttta gacaaggcag aagatgtcaa agagctcgtt
      901 gatttagact ttaaccgtgt catctatcta ggcgctggtc ctttctttgg acttgctcat
      961 gaagctcagc tcaagatttt ggaattaact gctggtcaag ttgcgaccat gtatgaaagt
     1021 ccagttggct tccgtcacgg tccaaaatct cttatcaacg acaatacagt tgttttggtc
     1081 tttggtacaa cgacagacta cactcgtaag tacgacttgg acttggttcg tgaagttgct
     1141 ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc aagcttttgg tcttgaaaat
     1201 gtcaaagaag tggcccttgg ttgtggcggt gtcttgaatg atatttaccg tgtcttccct
     1261 tacatcgttt atgcccaact ctttgcttta ttgacttcac tcaaggtaga aaataaacca
     1321 gatacaccgt ctcctacagg tacagtaaac cgtgtagtac aaggtgtcat aattcacgaa
     1381 tatcaaaagt aagacagtgt ttatgaattc ttgacaagag gatttgtaaa ttatcagata
     1441 aaccatagat tgtcagtacg ctttctatgg tttgtttgct tgagagaaat agtaaaagga
     1501 gaacagaatg aaagcataca cagagcgtgt atttggaaat gttgagggtg aggatgtctt
     1561 ggcctatcga tttgagacag acggtggcta ccaacttgag gttatgactt atggtgcgac
     1621 tatcttgcgc tatgtcgcac ctgacaaggc tggaaatttt gccaatgtta tcttgggatt
     1681 tgatgacttt gatagttatg taggcaatag tcccaagcat ggagcaagtg taggtcctgt
     1741 agcgggtcgt attgcaggtg cgacctttga gctcaatggt aagacctatg accttgaggt
     1801 taataatgct agcaactgta atcacagtgg ttcaactggt tgggattcca gcttgtttga
     1861 agttgaagaa gtaagcgatc atggcttgac tctctacaca gagcgtacag atgggacagg
     1921 agggttccct ggaaatctca agatttggat cagttatcac ttggaagaaa ctggtgccta
     1981 tgaaatcagc tacaaggtaa cgaccgatca ggatacgctg gtcaatccaa ccaaccacag
     2041 ctatttcaac ttgtctggtg atttcacgca gacgattgac cgtcatgtct tccaactaaa
     2101 cacagagggc atttactcaa tcgctcctga cggtgttcct gccaaaactc cagaagccaa
     2161 ccgtgatgtg gtcaaacacg tctacaatgg taccttgttg aaggatatct ttgcagaaga
     2221 agatgagcaa atccagctgg catcaggttt ggatcatcca tttgcccttc ctgcaggcca
     2281 tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc ttcctgcttt tcaagacaga
     2341 agctccttgc tttgtggtct acacagcaaa ctttgtggat gaaagtgtca tcataggagg
     2401 tcagccaatg ctacagcaca atgggattgc tcttgaagcg caagctttac cagatgccat
     2461 tcacagtgac cttaaaggcc aagtcattct taaagctggt caaaccttca ccagtaagac
     2521 acgttatgaa cttgttgtga aataaaaaaa gctggtcaaa aagtctttaa aattaaaata
     2581 acgcatattg tcaggtgatg aaaagtttga taatatgcgt tttattatga aatgatttac
     2641 tttatattat cctgaaatgg tgttattttt gagttatctg gcttgacttt cttgatggaa
     2701 attatataat agttgtaaca atgataatta caactagaaa agaggaccat ttatgaccta
     2761 tgaatacaag agtcacattt atttggcaga gacagcttta aatgtaaagg atttggccag
     2821 tcaaacagcc ttttatcagc aagttattgg tttagaaatc ttatctcaaa ctgagacaga
     2881 gtccattcta ggccttggtg gaaaagtctt ggtacagctg attcaagcac aagagagcgg
     2941 agaagtgagg gaatattatg gtctttacca tttggccatt ctcttgccca cacgcaaggc
     3001 tttagctgat gtcttgaaac acctgacaga tttacaaatt cctcttgtcg gcggtgcaga
     3061 ccatggttac agtgaagcta tttacttgga agatttggag ggaaatggca ttgaactcta
     3121 tcgagataag ccagtttcca catgggatat tcgagaagat ggacgcatta tcggagtgac
     3181 agaggctctt gcggcgcagg acatttatga gttgggggaa agagtagagc cctttatcct
     3241 ggcagaaggt acgagaatgg ggcatattca tctttccgtc aaggatagtc gaaagtccag
     3301 ccagttttat caaaaggtgt tagggctaga ggataaattc agtgtgccta gcgctagttg
     3361 gattgcggct ggagattacc atcatcattt agcagttaac gaatggggag gaaaaggtct
     3421 ggcttcgcgt aagcaaggct taccaggttt agcctactat gtcatcgaag tcgcacacaa
     3481 aaaagaacta ttaacgattg cccaacgagc acaagaagtt gacgcaccaa tcaaatggat
     3541 gacatcgagc caattggaaa tcacagactc agatggcatc gtgacccgta ttcgtttagc
     3601 aaggtaaaat taggaatttt taataagtta taagtattgc tagtttgctt tttgaacggt
     3661 ctttggtaac agaactgcgt gagctggatg ggcgagaatt ttgacaaaga agctgattag
     3721 agatataatg aagaaaaatc gagcaaggaa aaagaaatga caaattcaaa gtatattact
     3781 tgtttgaaac gttcagaggg ccagttgtgt gggattcaaa agatgattga aggagatcgt
     3841 gactgtgctg atattgtgac gcaactgaca gcagtgagat ctagtgtaga gcgcgtgatt
     3901 gagatgataa ttaccgaaaa tcttactgaa tgtatcaatc agccactaga tgattctgaa
     3961 gctcaaaagg aacgcttaga aaaggctatc cgatacttga ttaaacggaa ataaatgaat
     4021 gttttgagac gatatgtgca aaggtctatt aactattaga atagtttaat gggagtcaaa
     4081 agaagttgta aaaaatcttt aaaattaaaa aaacgcatag tatcaggtat tgaaatgtac
     4141 tgaccccaaa aagttagatt ttttctgtct aacttttggg gtgcagttca taagaacctt
     4201 ggtaatatgc gttttattat ggggagattt atacttttcg aaaatctctt caaaccaggt
     4261 cagcttcacc ttgccgtaga tatggttact gacttcgtca gttctatcca caacctcaaa
     4321 acagtgtttt gagctgactt cgtcagttcc atccacaacc ttaaaacagt gttttgagct
     4381 gacttcgtca gttccatcca caaccttaaa acagtgtttt gagcagcctg cggctaactt
     4441 cctagtttgc actttaattt tcattgagta ttacttcatc ttctcatgaa attgagtttt
     4501 tgcccagttc ctcttgatta ttctacttga ccaggccaag cattcatacc accttctacg
     4561 ttggtaacgg tgaggccttg ggagatgaga aattggcagg tagatgcaga gcggactcct
     4621 ccttgacaaa taacatagta ttcatggtca ggtttgagtt ctttgtagcc ttgctctaag
     4681 gtacttaatg gaagattttt ggcacctggt gcatgtcctg cttggaattc atgtgcttca
     4741 cggacatcga taagttctag attttcattt tggtattttc cataaaagtc agccatgctg
     4801 atactagttt ccatattcta ctccttctgg gttaaccatt ttgtatagtg aataagcgcc
     4861 gtcaaggttt tgggcggtaa atcctgcttg cttgaggata ggctctgcga tatagctgcg
     4921 caaaccactg tggcagctga cgatgtaggc ttgttttttg tccagttcat cagcttcctc
     4981 cttgacactt tgtcagtttt tgatacaata gtacaaaatt agaggaggtg ggctatgatt
     5041 cagaaacatg cgattcctat tttagagttt gatgacaatc ctcaggcggt tatcatgccc
     5101 aatcacgagg ggctggactt gcagttgcca aagaagtgtg tttatgcatt tttagatgag
     5161 gagattgacc gctatgcaag ggaagtaggg gcggattgtg tcggcgaatt cgtttctgct
     5221 accaagacct atccagtcta tgtcatcaac tacaagggtg aggaggtctg tctggctcag
     5281 gctcctgttg gctccgctcc agcagcccag tttatggatt ggttgattgg ctatggtgtg
     5341 gagcagatta tctctactgg gacctgtggt gttctagctg atatagagga aaatgccttt
     5401 ctagtccctg ttcgcgctct gcgagatgaa ggagccagtt accactatgt ggcaccttct
     5461 cgttatatgg aaatgcagcc agaggctatt gctgctattg aggaagtttt ggaagacaga
     5521 gggattcctt atgaagaagt catgacctgg acgacagacg gtttttaccg agaaacggct
     5581 gaaaaggtgg cttatcgtaa ggaagaaggc tgtgctgttg tggagatgga gtgttctgct
     5641 cttgcggcag tagctcaatt gcgtggggtt ctctggggtg aattgttgtt cacagcagat
     5701 tctctagcgg acttggacca gtacgacagt cgtgactggg gctcggaagc ttttaataag
     5761 gcgctagaac tgagtttagc aagtgttcac cacctttagt tgtactggca aaggatttgt
     5821 tttatcataa aatgtctagc tcatactttt caaaaatatg tttaaacgag gtcaccttcc
     5881 tcttgtccta agcatgttga ggttgggaaa aatctttaaa atcagaaaaa cgtatcatat
     5941 caggtgatga aaaccttgat actatgcgtt ttatgttggt aagatttaga gtgagatgaa
     6001 atgatactct tcgaaaatct tttcaaacca ggtcagcttc
//
LOCUS       DAVBCR010000096         5951 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCR010000096 DAVBCR010000000
VERSION     DAVBCR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.096"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            476..1495
                     /locus_tag="VQ508_001885"
     CDS             476..1495
                     /locus_tag="VQ508_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2490839.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            1508..2611
                     /locus_tag="VQ508_001886"
     CDS             1508..2611
                     /locus_tag="VQ508_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490840.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLTNIKTDYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIIDLEDDKK
                     RLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSVKQRIVS
                     SISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAKSGLLYN
                     SLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIFSMLQVS
                     SVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            2640..3548
                     /locus_tag="VQ508_001887"
     CDS             2640..3548
                     /locus_tag="VQ508_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490841.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            3550..5448
                     /locus_tag="VQ508_001888"
     CDS             3550..5448
                     /locus_tag="VQ508_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2490842.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            5472..>5951
                     /locus_tag="VQ508_001889"
     CDS             5472..>5951
                     /locus_tag="VQ508_001889"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2490843.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         2235 a          634 c         1032 g         2050 t
ORIGIN      
        1 gcacgtacgg tggtgtgaga ggggctagag attatcccct actcgattgt ctatagctat
       61 aggggtaaag ttattagaga ggttgctagt ttatagagac atcttgattt gtaaaaagtt
      121 aaattttaga caattatccc ctttaaaaaa tctattaaac catagctagt tgaagtaaat
      181 ttttatagaa cataatttta tttatcaaga cattgtagag ttgaggcaaa ttgaaaaaga
      241 aagtttaaaa gttgataata agtcaataag aaagctatta attctaacga gattatttga
      301 tagtaatttg ttttttcagc tactattcgg acaatgttat tataataaac acaggagaga
      361 acaccagaag gtagggacat cagaagaaat agttcaggat atagttaaca taatcatcaa
      421 agaagctcct agactcgcta atgttaattt ggaaacatat tatccagatg aatatatgtc
      481 aaatacattg aagcaattac tactttttag agaggagttc ataaaaaata ttttgaatgg
      541 gattgatgtg gatgaaacag tagtaataga aaatgatttt gtagaagagt tggagaactt
      601 tttaaaagag aatgatttgc tagatttttc aaatagcata ttttttcagt tatctggaga
      661 taaagtaata gtgaataata tttatccagg atatttagca tattttaata ggttccttac
      721 attttatccg aaaatattcg ctattaaaga agttggagaa tatattgact ttttaaattt
      781 tgaaaagaga attgcggata tgttttattg ttggggattt aatgcaaatt ctagagtgtt
      841 gacagctgaa aaaataattg aaatcccaaa tcaccaaatc aatgctaagt acataaaaaa
      901 agttataaaa atgagtgaac tcttgtttgg tgttgatagt aataacagaa ttcggttgct
      961 taacaataga agggaattga taaaaactat tttccaaggt tctgtaatta agtcagcaat
     1021 tcctgctcta gctgctactt tggatacaat cagtaacagt ggatcatttc aaatttcttt
     1081 atcaaagcag ataattaata atttagtatt aagtggtaaa gataaagtta caattcctag
     1141 gatagagtta gataatgttg ttctttcaag acagaagatt attgtatcag tttcgtatat
     1201 aaatgatttt ttggatttga gatttccaga taaaatagaa tacattttaa attttttaga
     1261 aggatctggt ttcaatacta aagtaatggt ctactttggg aaaattgtag aagaagataa
     1321 gaactatttt gttaatcttg aaaaacctca gtattttgat tttaccagta tacttttcaa
     1381 taagttattc tttaaagaat taaacaagtt tgattacatg gttattgaag aactcattcc
     1441 aaatcttgat gaagatgtaa tgatgacaga gtatataaaa gagtatactg aataggagca
     1501 atctattatg attttaagta aagtacgttg ttacatatat aattctaaaa ttagaggtaa
     1561 agttctgtat gaattatatg aatttataaa tgaaaataag caattgatgg acgcaattaa
     1621 ttcaattaaa gttgacagta gtaatggttt tagtattcaa atattacatt taggagaaca
     1681 gcaaacaact gtcttattaa cagatttttt tactaaaact ttagacttgt ttaaggaatt
     1741 tagggttaca aataagagta ctcttgagca ggtgaaaaat caaattagca tactaaattc
     1801 ttctggtcca aaaatggatt taacgaatat aaaaacagat tactcaatag tggaagaaaa
     1861 attagattgg caaagtaaat tatctaaaat ttatccgaat gttgaattta taaaaattga
     1921 aaaattaaaa ttgattttgt tgtgtaaata tagaaatcaa attattgact tagaggatga
     1981 taaaaaaaga ttgttactat tgaaattatt atatttgact tcaaagcaat tttgctatga
     2041 tgaatttaaa ggagcgaaat ttggatgtat gtcatttttt tcacattatc aaggttatat
     2101 gagcagatta gattcactcg actcagataa atttgtttcg gttaaacaga ggattgtttc
     2161 ctctatttct aaattagttg agcaatattc agatcatatt ataaatctgg atagaacttt
     2221 agttgattct ctaagtgata cccttgagaa tcagctattt gatgattggg attatatatt
     2281 aagcgaaata actacatata gcgaattatt agcaaaatca ggattgttat ataattcgct
     2341 agactataag attgataatg taaaaaaaga agtggccaag ctaagtgact ttcaccaatc
     2401 tttatttaaa attgaaggat tttcagattt ttttgatagt tggcaatttc aaagatatag
     2461 gtctctcgtg aaccaagttt atgaaatttt ttcaatgtta caagtaagta gtgtagaaag
     2521 aactgtgttg tgcggagctc taagtactat tgtctttagt aagtcaaatt ttaactatca
     2581 agcttttttt gagtatctaa aagatacgta gaattcgatt ttaaaggagg ctgaaatata
     2641 tggattatgt agatgtaata ttggatgagt atgggagtgt ttttctgata aaggatcaca
     2701 taaaactaaa aattaataat ctagaactat atcggtattt tagtagcttt ggaatgagtc
     2761 atcttcaatt tagaaaagaa gagcttccag gaaaattaaa gcagtttgat tttgaaaaaa
     2821 ataaaagtga cagttatttt tctaagaaaa gtagtctgat tgattttatt aagagaaatt
     2881 atttttatga tattaagtta tattctgata atccgaaaaa tccgatagag tcttggaaaa
     2941 ataagacaga aaaaattaag attgctaaaa gtacagaaat tctttggagt gaagtagcaa
     3001 atttttcgtc aatttctaaa tctccttact attgcttgtt atataattta aagaatactg
     3061 aaaattacat attgataatc agtaagacat atttttcagc catccaaaaa atggtttcgt
     3121 ataatctaaa taattatttt gaattagaag atgtttgttg taaaagcata gtgagcaagc
     3181 gagaattgat agaaaattca attaatatta acaaaagcaa aatgaaactg ctaaatgttt
     3241 gcgaaggtag taaaggttac acaagaagag tatttaagta caataatatt tttgtgggac
     3301 aattggaatt tcactctaac ataatctttt cagaaattgc tttaaatgaa ttaagtattg
     3361 ttaagctttt agattctgtt gaaagtgttt tatctgagca actatttgga aaattaaatt
     3421 gggataattt atttgttgtt ttatcagatg attttaatga ttatctggat ataaatgaat
     3481 ctcaaaaata ctactcaaat ggttctttga gaggactgga gcttggaatc tttatgggag
     3541 gggtttaaaa tgagaataat tgaaattgtt gatgttgagg accaactttt acaactttta
     3601 agtgcacata ccaatattga tattagagca caagagttat caacacttga cccagattca
     3661 aaagttgatt tgatcatata cttgaaatct cgacgtgata ttgaaaacat aaataatatt
     3721 attattaaat cacctaacag gaatatcatt gttataataa gtgcttttga tattatatat
     3781 gcattgtatt ttccaaaatc aacatttaga ccttgtttga actgtctatt attaaaactt
     3841 aaagataaca accctgagca taatctatta ttattcctga aatacggaac attacctagt
     3901 aaacatttta atcttgataa taggttaaaa tcaaatttaa tactatttaa tgagaagtta
     3961 gaccctaagg tagaggagta tagtattgag ggggtaaata ctacaaattt tactttccat
     4021 tttagttgta gctgtgaaaa tagtggttac gatatgtcgg aaagaatggc agatattcta
     4081 cttggtgaag gaaaaaaatt atctcctaaa aattatcgtt taaaaaagag tgagcatcta
     4141 ttcgatttta ctagatatct agatagaaat tttggatttt ttcatcattc atatgatgaa
     4201 aaaatctccg aatatgcgcc tctccatgca gtagagatta atgttgatgc tggttacgag
     4261 tcaggctggg gaaggacttt tatgacagag gctgcaacaa aatctgcttt tttagagtca
     4321 ttagagagat actgcaactc tagaaataga cgctataaaa aattaattag agggtgtttt
     4381 tctgatttat ctaaaaagta caatacatta aatcctgttg atttttgtct ttctaataaa
     4441 gaatatagag atagttccta taaatttcat gattttgatt ctgagaaaaa ttatgattgg
     4501 atatttggta aaaatataac taccggaaaa atttgcttat tgccctcgca actagtattt
     4561 tataaagact ttagtaaaag cgaagaaaat gaaaggtttg tttatgaaac atcaaatgga
     4621 tgtgctttgg ggagtagtat tgaggaagct attttttatg ggattttaga gattattgaa
     4681 agagataatt ttttacttac atggtattta aaagaagaag taaaacaatt atcatgggaa
     4741 tctttaccat ctcgcctacg tattattaat gaaagactaa aagaagataa ctgtgagctc
     4801 aaacttttta atataactaa agaagtgggc ataccagtaa tttgggcatt gtatataaat
     4861 cataatgaaa atgctagtat tcgaagtttt tcagctgcag gctgtcatat taatccttta
     4921 agtgcaattg aatcagcaat ttttgaagta ataacttctg tgccaacttt tgaaagatta
     4981 attgaagtta gaaataaaga gtcatttatt gaggaaattc agaataattt ttctttaata
     5041 cgagatattg acgatcatat attgtatcat gctcatgcgg attataaaaa taaaatgccc
     5101 tttttattta aaaataatta tagtgaatat caaaatgaat ttgatgagaa tagaattttt
     5161 aatacttatt cacatgcaac gcttacatca gatttacagg cactaataaa aattattgaa
     5221 aaaaaatttg gagatatata tgtagtgaat ataagtgata aagtttccga aaaatttgga
     5281 ctttattgtg ttaaagtgtt gattcctgga cttcttaata tgtcatttgg acattcaaat
     5341 cggcgtgtaa atatagagag gataaaaaat tatgtagatt atagaggggg aaatgtaaat
     5401 agtattacat tgaattccat aaaccatctt ccccatccat ttccataaga ttatatataa
     5461 aggaataata aatgaaaaga gattggaaat caaagcattt attttatcat gatgttcaac
     5521 aatttgataa attggtaatc tatttatcaa aatttatgga taaattagtt aatcagaatc
     5581 agattgaaaa atatttctat attcgttatt gggaaggggg gcctcatatt cgattaagat
     5641 atttggtgag ggatacacaa aattttgaaa caattgatag tatgataaat aagtatgctt
     5701 ataaattttg ggaaaataat cctccaatga agttattaaa taagaccgat tataagaaaa
     5761 tttataatga gagtattgat aacaacggaa agatggatag aaaattgagg aaaaatgcga
     5821 ctattgaaaa cattgcctat ataccggaaa tagagagata tggcggggaa gaagctctga
     5881 tagtaagcga agatctattt tgtatctgta gtcaggtttc aactgcgtta cttaatgtgg
     5941 tagataacta t
//
LOCUS       DAVBCR010000097         5804 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCR010000097 DAVBCR010000000
VERSION     DAVBCR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.097"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(119..751)
                     /locus_tag="VQ508_001890"
     CDS             complement(119..751)
                     /locus_tag="VQ508_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2490844.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ508_001891"
     CDS             complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ508_001891"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2490845.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ508_001892"
     CDS             complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ508_001892"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2490846.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2567..3139
                     /locus_tag="VQ508_001893"
     CDS             2567..3139
                     /locus_tag="VQ508_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2490847.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3191..4054)
                     /locus_tag="VQ508_001894"
     CDS             complement(3191..4054)
                     /locus_tag="VQ508_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490848.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4520..4858
                     /locus_tag="VQ508_001895"
     CDS             4520..4858
                     /locus_tag="VQ508_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2490849.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4836..5195
                     /locus_tag="VQ508_001896"
     CDS             4836..5195
                     /locus_tag="VQ508_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490850.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5214..5426
                     /locus_tag="VQ508_001897"
     CDS             5214..5426
                     /locus_tag="VQ508_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490851.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
BASE COUNT         1783 a         1002 c         1215 g         1804 t
ORIGIN      
        1 atctacaacc ttaaaacagt gttttgagca gcccgcagct agtttcctag tttgctcttt
       61 gattttcatt gagtattact tcattttctt ctgaaataga gttgtcgccc agtctatgct
      121 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
      181 gtagatctgc ttgctctcca aatccccaag tgacggccaa tttctgaata cctgtttctc
      241 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
      301 gatgtgtctg taaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
      361 cataaatacc atcaaagaaa tgatggattt ccaagttttt ggccatgtct tgagcagtag
      421 atgtattctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
      481 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
      541 atatctgcat ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
      601 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
      661 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
      721 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
      781 cgaaaatctc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
      841 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
      901 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
      961 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     1021 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     1081 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     1141 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     1201 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     1261 cagcagtgag aacggaaggg agacaaccgg cacttcctgc ggtaggagta gcgcagacca
     1321 agcccatttt agcattgtgt tcattgactg cgatagcatt tcgggcagca gagagaatcg
     1381 tgtaatctga taaagttttt tcgtttttga tgtagtgatc caatttggca gcatctccac
     1441 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     1501 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     1561 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     1621 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     1681 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     1741 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     1801 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     1861 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     1921 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     1981 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     2041 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     2101 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     2161 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     2221 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     2281 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     2341 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     2401 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     2461 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacagtaata
     2521 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     2581 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat tttctccatc
     2641 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     2701 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     2761 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     2821 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     2881 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     2941 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3001 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3061 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3121 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3181 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3241 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3301 aatcgtctgt acaaatccca atacggacat agcagatagc cagcctatcg tagcttttct
     3361 tattcttggc gtcctccagt aaagtgtagc agatttgctt acacatgttt ttatcttgat
     3421 tgtatagata gagtgtggaa aggtttaata gaattgtcat tcgcaagtca tataaatgtt
     3481 gatagttttt atagacttct aggcgttgca atatttttcc agtgatgaga tggaggtatt
     3541 caatgggaaa actgaaaaga atggtattga ggatttttag atcactctca taccatgtat
     3601 cttgtttttc aatttttttc caaagttttt tgacaacctg ttctgcgtgt tttgatagtt
     3661 cccctgcccc gtgttgacgg agataaataa caacttcaag catatctctg atttcttcta
     3721 tagggagatc gtggtgggtc ttgaggtagt cttggcattt ttgaaataaa ttgacaagat
     3781 cgctggtccc gataatggaa cgcatattga gataggtttg cataatctct gtgcgttgac
     3841 ttggttgata aagttgacag atatattcaa actcttcaaa actcatattg atttgacgga
     3901 gaagaaattc catattttca tattttgggg ttgtcttgcc actttcgatt tttgataagc
     3961 tggttcttga gagaacaccc ccacagacct cttcttgggt cagccccttt gactcacgta
     4021 tttctttata gacctttccg aaatcataac gcatgatttt ctcctttctg tgaaaaaagt
     4081 taacaaacaa taaaattttg ttaaaaatat tgtatcatag caatgtaaaa agtaaaaaag
     4141 aaagtcgaaa taaatttgtg aaaaaagtta acgtaatttt aaaatgagct taataagagg
     4201 tatgatgtag gtagaaaata aagataggag aaaaagtatg aataaaaaaa tattttgtat
     4261 tctagtatgt attatatcat tgattagtct ggccataatc tttccatggg gctggtcgat
     4321 ataatgtaag ggagcttgag ttgttaaagg aggcttagtc cttggacaaa aatctgctgt
     4381 actacaatgc ttggaaagat atcaagaaca aagaatgata cgattgaaaa taaactgatt
     4441 caagaactac tgggcagtga ttgaggtttg ggtgacatca aagaaaagtt agattaattc
     4501 taaagaattg agtatttcta tgttaaaaaa atatccttgt acgatgcaac atgatcagtc
     4561 agattgtgct gcggcagttg tttcaacagt tcttttatct tacaaaaagg aactatcaat
     4621 catgaagatt cgggaaatta ttggtacaga catgtatgga acgactgtca gtggtattgt
     4681 ttcaggtctg aataagttga attttacagt aaaagctgtt cgagtagcac tggaagattt
     4741 gactccaaaa ttaacatttc ctgcgattct tcaagttaag aatgatttag gtcaaaatca
     4801 ttttgtggta ttacatagta taaaggagaa aataaatgga actcgtatta ccaaataatt
     4861 atgttgtgat tgatgaagaa gagatgatgt accttgatgg gggggctata tatataccca
     4921 ggtgggcaat tacaggagcc attactggtg cagcatatgc agcattagca gcagcaggag
     4981 gtggaggcct tcaactagtt cttgcatctt atggattacg ctccgcactg gtagctggga
     5041 ttgttaaagg tttaggagta ttaggaattc atattggaaa tgcttttgca aatactgtta
     5101 ttagaagtat tgcatctgct ggaattggtg ctggagctga ttggatttta ccaatattat
     5161 tgatggctgg gatgggcgac gtgataatca attgagaata ggataatagt attatgatgg
     5221 ttgttttaat ttcagttatt gttgcattcc tgtaccataa aattacagag tatgtcaaca
     5281 acaggaaaat tgatattgtt atttttctat cattgctgat ttctcatttt atttggaatt
     5341 actatttttt accaattgaa atattgatag caatcagtgg agcaaattta ttattaagtt
     5401 ttttaaaaaa attacagatt aaataatatt attccatatg acatatcaac taggtcatta
     5461 gattttattg aagatataaa ttaaaaacga ggttgggaaa aaactcaatt tgaaagtgaa
     5521 attgagtaaa tttttctatt atataacgca tattatcaag atttttgaac acctgataat
     5581 atgcactttt cttattttaa agattttttg cctttcttca tttcttgaat gaattggaga
     5641 gatgacttta tttaggtgtt tgacattact atagatagga tttagcagta ctaatatgaa
     5701 ctcatattat aggtgcagac ttaaattttg gaagcaagct aaaggatatc ttatctaggt
     5761 aactaagtgc tataaacaaa gtcaacgatc acttgttagg aaac
//
LOCUS       DAVBCR010000098         5771 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCR010000098 DAVBCR010000000
VERSION     DAVBCR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.098"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..1412)
                     /locus_tag="VQ508_001898"
     CDS             complement(<1..1412)
                     /locus_tag="VQ508_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2490852.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNAN
                     GAMATGWLQYNDSWYYLNANG"
     gene            complement(1962..2201)
                     /locus_tag="VQ508_001899"
     CDS             complement(1962..2201)
                     /locus_tag="VQ508_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490853.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            complement(3336..4064)
                     /locus_tag="VQ508_001900"
     CDS             complement(3336..4064)
                     /locus_tag="VQ508_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490854.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            complement(4061..4300)
                     /locus_tag="VQ508_001901"
     CDS             complement(4061..4300)
                     /locus_tag="VQ508_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2490855.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            complement(4356..5258)
                     /locus_tag="VQ508_001902"
     CDS             complement(4356..5258)
                     /locus_tag="VQ508_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490856.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            5512..5670
                     /locus_tag="VQ508_001903"
     CDS             5512..5670
                     /locus_tag="VQ508_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490857.1"
                     /translation="MAQIVVIASPIDIQAANPLIADSANPATLFATRTPTYLAAAPAI
                     ALVTRRKP"
BASE COUNT         1840 a         1100 c          819 g         2012 t
ORIGIN      
        1 ccgttagcgt ttaggtaata ccatgaatcg ttgtattgga gccaacctgt agccatagca
       61 ccattagcgt tgaggtagta ccatgaaccg ttgtattgga gccaacctgt agccatagca
      121 ccattagcgt tgaggtagta ccatgaaccg ttgttttgta gccaacctgt agccattgaa
      181 ccatcagtat tgtagaagta ccacataccg ttttcttgtt tccaacctgg ttgtggtgca
      241 ggagctggtt tttctgcttt tggcggttgc tgttgggtca agcgattata ttcttcttct
      301 gatctacgag catagtcttc ttcagcttgt tgatctgctg acttctctgg ttgtggtgct
      361 ggagctggat tctcaggctc ttcagctggt ttttctggtt gaggagccgg cgctggtttt
      421 ggttgttcag ctggttgctc tggttttggt gctggagctg gagtttcttc agctggtttt
      481 tctggctcat taactgcttt cttaaggtca gcttcagttt tttctaattc agctttttta
      541 gcaactaagt cttcttcagc tgcagcaagg tattgtccag cttgctcacc gtctgagttt
      601 ttgaaatctt ctacatcttt ttcaagtttt gcaatttcag cgtctaactc atcaatctta
      661 tcactcaact cttcaagttt tgatagttta gcttgcttaa catccaattc aaattgaaga
      721 ggagcacgga gaccttcttt aacataatct tctgaatcag actcatcaat ctctttgaga
      781 gctttttcta gtttctgaac ttcatgttcc aattcagcga ttttagcttg aggagcgact
      841 tcttcagcat gttctgcatc cactttttgt ttggcttcag taactttttt ctcagcttct
      901 tctacttttt tagtaagttc tgcttctttt gctttagcct cttctgcttt ttgtctagtc
      961 ttttttaatt cttccgcgct aggaattacc tttgcacgaa cttcagcaaa ttccgtttgt
     1021 tttttcttat actctttctc agcctcatcc attgctttct tagcttcagc tttttttgtt
     1081 ggatcatttt cacgtatata tttaaccaac tcttgttgat atttcagatt tgcagcttgt
     1141 tcttcttcag aagcttttct ttcttcttcc gctttctcct cagttttctt ctgatcatct
     1201 tcatactttt tctgagcagc ttttccgtcc tcatattcct tcttagcagc ttcatatttt
     1261 ttcactgctg catcatagtc tttctcagct ttagactgac tagctacggg agcttcttct
     1321 gctcttacaa aagtaggttg agacgtaaca aaaccagccc ctaagatagc gacgctggct
     1381 agacttgtta aaatcatttt tttcttattc atctaaattt acctcttttc tgatagtcat
     1441 ttttagacta tacttatatt aagtatactc cccccgtaaa tatttgtcaa atgttttata
     1501 ttacattttt attacaaatt tctatatcat aagcttctac tccaaaaaga taaagacact
     1561 acttttgccc ttttcgattc tctttttaaa catagcatca tcttccttca ttcctataaa
     1621 aacatttccg atgagagagc ttgtttttta atttcgttaa acaacaaaaa acgcaagctt
     1681 aagcctgcgg acaatcttta tagccatttc atttctttca catctgtttt acgaacttta
     1741 gcatatttct tagcgcttgt aaaatgtaga gtaactggtt taaatactcc gcttttgtct
     1801 cttcgtaaac ctaaaactgc ggatctttta tttttatcct caaaaaatat gacgtccaat
     1861 ttcatagtat ttttagataa atccttagct acgatgcaac aacttgctag cttagatata
     1921 gaagatatcg ccgataaact cacataaaga tatcctttcg attacatcat tgtacatagg
     1981 atatttctaa aatcttctaa aataaaaaca ccatcttgaa tagcaggttt tgtttgacta
     2041 gcataatctc gtgtgatttt gtataagtca aataaatgat gtaaatcagt caaacaaaaa
     2101 ttcactttga aaatctctaa atacttataa ttcgttgata cttgacatgt ttttccattg
     2161 aatctttctt gatagtccaa aatacaatca agtaatttca acgacaaact ccatattttc
     2221 tcaaaataaa aaagcacggg gcagttctca ctattgccca aagtggttga ttagagtcgg
     2281 cttctctaac tccctatgtt gtgcttatta ggcttatcgc aagggaacag tttttagctc
     2341 tgccaagcca catagtgtgc ttactactat gtgtccatgt caccatgaat tgccgaacgg
     2401 tttaacgaga aagtatagta gattgaaaca agatgtgaac aaatcgatta ggaaagtcaa
     2461 attaatttct agaaatattt taacagtcat aacggactat tccagattca atctactata
     2521 ttacttttag gtttatccca actcaattat gacatctttt tttcaaaagt caatatatct
     2581 cactcttttc aacgacaaga aagaggctga taatcgacca acctctgttt ttagatacag
     2641 tttaagtcca aactattctt tatagaaaag tccgataaat tcgagcaata gttaacatac
     2701 ttacaaatat aactcccaaa atagataaca ctattttcac aaaactcagt tccgaaccta
     2761 tgacatatat atgaaaaaga ggggctagtc ctacacatat atacaataag tagccaacaa
     2821 gttttatctc accaattcct gcaaaaaaat gtttcatgta acgattctcc atttactcta
     2881 ttaaatcaat tttattttag actatcataa cacaatcacc tcttcaataa aaaccattgg
     2941 cgaaatacta ttctgaagca ttgatagctc agatttctca gaatagagct gtcctaacta
     3001 tcgagcagct caaatacata tatatgagca attctaagcc tagaattgct ataaaataaa
     3061 tgttttcagg ctattctaag tctttgactg cttaaaattt tacgttctga aggattctaa
     3121 agctagaata gctcaaaatc acgagaacta aggtattcga aacttcagag taatacaatc
     3181 cccataggaa ttctccattc ttattaagca tgattaaaat gtaatcctaa caagtgatcc
     3241 tcgattgtgt ttgtagcact tagttaccta gataagacta tttcccttta tcatatctga
     3301 ttttaagccg ttctaagatt taatccatat ttctattact tgctattttt aaataattcg
     3361 tattcctttt ctttgataaa tggaactgaa aaattagaaa aaagaagtaa aaccgaaaac
     3421 aaatagcctg ctttttgaat cactaaaaga tatcctaaaa ataagtaagc tataacatcc
     3481 aatgcgaaaa gaaaaaatat tctcctcttc attaaagtac ttgtgtcaaa taatctgatg
     3541 aataaaaatt ttatcgtagt atctacacaa atttttatca aaaagataag agtcagtaca
     3601 tgattatcaa ctttaccata aagaatagat ggaaatatag ttaatataaa actagaaatc
     3661 tctaaatatt ttatccaagg agattgagac accttgcttc taaactgact aattttctta
     3721 tattcgttag tcaatttaat atcaaaatct ttcctaatta aatgaaagat tgctaactct
     3781 aggataaaaa cacttattaa tacggtataa gtcaaaaaac tttctgggaa aaacttattg
     3841 attaaaatgg ggaaactcat taacagaatc gaatttataa ctagacagat agatactatt
     3901 ttatttttca cagaatatag aggtagtgat actattgagt agataaaagc taaaaaaata
     3961 agcaaaaaat actccgtatc atttataatc gccctgaatg ataaaaatat atacacccaa
     4021 cacacgatga tgtgtatttt tcgaatctta tctacattac tcatcttact gttgcagata
     4081 tgaatccatc ccaaggataa gggttctcgt taatatcaca acctctatta agaacaccat
     4141 atccaataca gtaagctatt ttccctgctg ctgtagccaa tacacctgct gctgcgcaaa
     4201 ttccttgaca agcacgcttg cttaaataag ctcccccatc aagatacatc attacttctt
     4261 catcaatcac aacataatta tttggtaata caagttccat ttgttttctc ctccttagtt
     4321 gaaattgttt catttgtttt acaaatagga cgaacttaaa ctatcttttt attactcagt
     4381 aataatttcc gagatttcat tttatgatac tctctattca tacagaaatc aaaattttgc
     4441 cttaaccaac ttgtataata gaaaaaatct aattgattga ttctgatgag ctgtattcta
     4501 attttatcaa ccttttcttg ccagttgtta ttgttactaa tagaaatctc atacagcagt
     4561 aactcgacaa gacctgtaca cagaagggtg ttatgagaat cttcgatttt tttcaaaata
     4621 tcctcaaaaa gttcactatc cgttaaaaag gtggattggt tcatcgcatc atagagaaga
     4681 agggacatta agtcagaata gcttaattct gatttttgta ttttgtccat caatatctta
     4741 tgcaaattac tttcaaatga atcttctttt tgccatttgt tttgattaaa aagaaattcc
     4801 gtcttattaa aaacgagatg attgatatcc attatagtcc agaaaaatat tgtagtaagc
     4861 atgatgattg caaaaggaat ttttaaaata aatactagga taatagcaat ttcaagaata
     4921 aaactccata taatcctaga tcttaaatca tcaggcgaca aacttttacg atggaatgaa
     4981 aaatagggcg agaatctcac attcactttt ttccttctta taaaaataaa gggaaaaatc
     5041 ttaataagac cttctttctt gtataaatct ttcaatgcgt atagtatgtc taaaactatt
     5101 tttgataaaa tcaaactaca gaaagcaaga ctcatactaa tcacaaaatc aaggggagat
     5161 tctagattca atagtaagtt attcactagg acatgcaact ctagccacaa gacaaaaaga
     5221 gggagcatta gtaagaaaac aattagttta cgtatcataa ttttacagtg tccttatcat
     5281 tttcttaatt ttggataaat catcattgct atgattaaaa tcaaaaaaac gacattaatc
     5341 caaataatca acttttttat ttcttcgcta acaaaatcat aggctaaaaa agttatggat
     5401 gatataaata gacatacaaa aaaaataata acaaaaattt tttccatttt aatacccata
     5461 tctagttacc acaaggcgtt taaatttggg tctctcctat ttacatcatt gttggcccag
     5521 attgttgtaa tagcatcgcc aattgacatc caagctgcaa atccattaat agcagacagt
     5581 gcaaatcctg ccactctatt tgcaactcgt accccaacat atcttgcagc tgctccagca
     5641 attgcgcttg taactcgccg caaaccataa ttacgaatat aatttctaac tagactgatt
     5701 gctcctccac ctaaaacacc attaaaagct atattaatgg ctgttgcaac aggccatctc
     5761 ggaatagaaa a
//
LOCUS       DAVBCR010000099         5738 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCR010000099 DAVBCR010000000
VERSION     DAVBCR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.099"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(436..960)
                     /gene="grpE"
                     /locus_tag="VQ508_001904"
     CDS             complement(436..960)
                     /gene="grpE"
                     /locus_tag="VQ508_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2490858.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(987..2021)
                     /gene="hrcA"
                     /locus_tag="VQ508_001905"
     CDS             complement(987..2021)
                     /gene="hrcA"
                     /locus_tag="VQ508_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2490859.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(2185..2694)
                     /locus_tag="VQ508_001906"
     CDS             complement(2185..2694)
                     /locus_tag="VQ508_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2490860.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            3302..5240
                     /locus_tag="VQ508_001907"
                     /pseudo
     CDS             3302..5240
                     /locus_tag="VQ508_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            5238..5738
                     /locus_tag="VQ508_001908"
                     /pseudo
     CDS             5238..5738
                     /locus_tag="VQ508_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         1821 a         1085 c         1103 g         1729 t
ORIGIN      
        1 actcttctta agtggcgacg ccgtcagagc ttgacttcgt caatctcttt gactaaactt
       61 tgagcctaag gtctcaaaat ttgcgcaaat agcaccacgg cgaagagtat cgtctttggt
      121 tcgcttcgct cactattaca aagccaaaca tattttatct ttcttcatag tttattgtcg
      181 tttcggacaa gttttctatt atgtaaattg cgacacgagg agtcgaaatc gattttattt
      241 cgacgacgag ttagtaagga agctaggcaa acgtcatagc gattgccgtt ttctgacgag
      301 ttgctttagc taccgtcaga attgccgaat cgaacacgcc ctaagctctg agtaaaaaag
      361 atagattttc ttgtgttcat cgaacacttc gtcaatctcc tatttttact gcgagctttt
      421 tacgagcttt gtatcttagt tatacaccac taccattgct gggcgtagga tgcggtcatg
      481 gagtttgtag cctttttgaa agacttgggc gatggtatct actgggtgtt catcgtctgc
      541 tgggagagtt tggatggcca tatggtagtt atggtcaaat tcgccatctg ctgcgatttc
      601 ttcaattcct tcttctttca aagcgtgaat caagctttct tgcaccatcc ccaagccctt
      661 cttcacatca tctgtcaaac cttcaactgc aagtgcacgc tcaaggttgt caagagatgg
      721 taagattgct tttgccaagt cctggctacg ataacgttgc aagttttgac gttcttcatt
      781 ggcacggcgt tggatatttt gcatttctgc atgagcgcga agatatttgt tttcgaactc
      841 atctgcacgt tcatttgcca agtccaactc agacttttca ggagttgttt cttcagctgt
      901 ttccacaact tcctcttctt gaacttcttc tacttcttca ttttttatat cttgggccat
      961 gttcgcctcc tttaatgatt tcaatcttaa tgtacttcgt aatgattact gctgaggtag
     1021 cggtaaaaat ctgtcaactt catggtcaaa acacggttga ccacattgac ttgattgatt
     1081 agctgttggt aatccagatt aactggaccg ataatggcta gaattccaac tccccgataa
     1141 ggaatgagga acttactact aatcaccgct aggtcagcta aacaggactc ttgaccgtct
     1201 gcaacacgaa cattttgcat ctgatcctca cgcaacccct cacgaatctc caaggccacc
     1261 ttttgcggtt ggtcaaagaa ctgataggct tctagattgg caaaattcaa gagattgacc
     1321 ttgcccgcca tcacaatgtt ttcgttgaac atttccttaa agatgtgttc aaagagatcg
     1381 atgacattat ccgttgttgt aaagtaacgc tggataatct gcggaatctc cgtccgaatc
     1441 ttgtagtgaa tatctaaaac ggtgtgaccg aggaaacgtt cctgaatgat gctcttcagt
     1501 ttcagcaaat cctcctgcaa gaagttcctt ggaatcagaa actgactagt aaccgttcgc
     1561 gactcgtcta gggtaaatac cgccaaggct gtatgttgcc ccaaaacaac gatatcaaag
     1621 gctgtcaaac gttgcctgct cggctcaaca tccagtgcca ctaccgtaca gccactcagg
     1681 cctgttagca agttagcagc ctcttgcaga atatcctcca atttgaagaa ttcctgatca
     1741 aaggctttga caatctcata tacctcattt tcagccagcc ggtgaaaatc cagtgagtgt
     1801 ttcacatagt actgaaaacc agcaacactt ggcatccgac cgcttgaagt atgagccttc
     1861 tcaagcaacc cttgtttttc tagttccgcc atgtcattac gaatggttgc actgctagag
     1921 ttaatagact cttgcaaggc ttttgatccg acaggttcgt gcgttttggt aaagatgtca
     1981 ataatcagat ttaaaatatc ctgctgacgc tctgtaacca tctcatcact cctctctgtc
     2041 tgatcaatta gcactcaatg catccaagtg ctaacttatg attctattat acacccttaa
     2101 aagagaatgt caagacaaaa actcaaaaaa ttagcactct tttatcaaga gtgctaaaaa
     2161 tcaatctgaa gacctagaac cttgctattc atctactcgg tatttccttt ttagtctgaa
     2221 ataaccatga attagataag aaatcagaaa agcatataaa acaaaaataa gaaagaaaga
     2281 catagaaaga tcctctctaa acaaactcgt acaaacaaca agaccaccta agaaagtagt
     2341 tgtacaggaa cgaagtctag atcgcataac ttcccatttg agccccttac tcatatagac
     2401 ttgatcctct ggaagtaaat cagaagacga tttacgaaga atcgaacaag aacctgctcc
     2461 tatcaattcc caacctctat ctctaaaatc ttgcagttca tgcttatatt ttttaagaaa
     2521 tctagaatca tagatacggt agatgacatc gtctggttga cattggtcaa aatagaacaa
     2581 accaaaacga ctcgttctat acctccaacc tttcaaatgc atctcatgta aatattcttc
     2641 ttccttgtcc aaatcaacaa tggtgaaaat ccgaaattct actctgctat tcattgtctt
     2701 accccaaaat tagaaaacat gcctggcgtt atttattaga taattctttc cacttttgac
     2761 tcaatctcca aaaaatataa gaaatctgaa tcgcaaaaac tatcaataaa acccaatcta
     2821 ttatgaaaat caaaaacact ttccaactga aagaactacc tccagtgaca aactttgagc
     2881 aaaacggtag tagagctaaa aagagaagta aaataggaag catccgcatt gttaaaatcc
     2941 gtttggcata aaaaaatctt tatttaaact aaaatattat agcaaaattt acgccagttt
     3001 ttgaacggct gatgtagata ttttatactt tcaaaatgtt taaatgtgat tatttatttt
     3061 tgaaaaatag ctcaccagcc cgactgaaag tgcttataga atgataataa gtcgcctgcc
     3121 gaaaacagcg gaaaatagcg gtgttatgcg gagataatct gacgcgatgc gaaagtatat
     3181 tgcatactta ttttcaacaa tttagcagag tatttttata agtgtgatat aatagaagta
     3241 taatttgttc tgatagttta ttttatggag aagtagattt ttagaatgcg gagggttcaa
     3301 tatggttgag tttataaagt ctaagaaaga aatgagtgag gaggatatta aagcaaattt
     3361 catcactcct gctattgtat ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga
     3421 atacttcact gatggtcgaa ttgaggttag aggagataag gctcgtcgta aagaaggaaa
     3481 aaaatcagac tattcactgt attaccaatt tggaactcga attgcaattg ttgaggcaaa
     3541 ggataataaa cacagcgttc gagcaggatt acaacaagct attgaatatg gagagatttt
     3601 agatgttcca tttgtttatt cttcgaatgg tgatggcttt attgaacacg accgtatcac
     3661 gagagaagaa cgtgagctgg agttagacga attccctact cgtgaagaat tattttctcg
     3721 tatgacgaag gaaaaaggat tgacgtacga aattacagaa gctatctcaa ctccatacta
     3781 tacagacgcc ttctcaatga aaacgccacg ctattatcag caaatagcta tcaaccgtac
     3841 tattgaaaca gttgccagag gacaaaaacg agtaatgttt gtgatggcaa caggaacagg
     3901 gaaaacgttc atggcttttc aaattattca tcgccttcga aaagctggtt tggctaaacg
     3961 agttttattc ttagcagata gaaacatctt agtagaccaa acgatggctg aagactttaa
     4021 gccattcgaa aaggtaatga cgaaaattac accaaaactt ttgactgctc ctgaaaaatt
     4081 aaattctttc gaaatttatc tagggcttta tcagcaacta actggtgaag atggaactga
     4141 aacacattat caaaaatttg acaaagactt ctttgattta atcgtaattg atgaagcgca
     4201 ccgtggttca gctaaggaaa acagtaactg gcgtaaggta attgattatt tcagttctgc
     4261 gacacagatt gggatgaccg ctactcctaa agaaaccaag aatgcttcca atacggaata
     4321 ctttggtaag ccaatctata cttatagttt aaaacaggga atcgaggatg gttttttggc
     4381 tccatatcgt gttatgaggg ttaatttaga tgtggatgtg gatggttatc gtccagaaac
     4441 tggaaaagtt gatgctaacg gacaattaat agaagatagg tactacggca ggaaagattt
     4501 tgataaaacc attgtcattg atgatagaac gcaaagagtt gccaagtttg tttctgatta
     4561 ttatgaagca aaacaatgca cgatttgata aaacaattgt tttttgtgtt gatattgacc
     4621 atgccgagcg aatgcgtgct gcacttgtaa aagagaatct agacttagtc caagaagact
     4681 atcgttatgt catgcaagta actggtgaca acgctgaagg aaaagctcaa ctggataact
     4741 ttatggatgt caattctaat tttcccgcta ttgtaacaac gtctaaatta ttaacgacag
     4801 gagttaatgc taaaacatgt cgtttgattg ttttagactc taatatccaa tccatgactg
     4861 aatttaaaca aattattggt cgtggcacac gtctttatcc tcaaaagggg aaagaatttt
     4921 ttacgattat tgattttcga aatgttacca atttgtttgc tgaccctgat tttgatggtg
     4981 agccagtgaa ggtgctggaa acaggtgcga aaacagtcag tggttctacg cctggtttcg
     5041 taggtgagga aggtgaccca gtagaaaaat atatcgttac agacaagcag gttaccattc
     5101 ttaattctac tgttcaagta ttggatgaaa acgggaaact gattaccgaa agcctgaccg
     5161 actacactcg aaagaatatc ttaggtagct acgccacttt gaacgatttt atcacagttt
     5221 ggcatacggc agataagaaa cattatggga agtttgctga tggaagcact caagaaattg
     5281 atgttcctta tgatattcct gatacttggg agtgggtgag gttttctaca ttggttgaaa
     5341 ttgtcagagg tggctctcca cgaccaatca aagattatct tacttctgaa gtagatggaa
     5401 taaattggat aaaaataggt gatactgaaa agggtgaaaa gtatataaat aatgttaaag
     5461 aaaaaatcaa aaaatcaggg cttaacaaaa ctagatttgt aaaaaaaggt acatttttgt
     5521 taactaattc tatgagtttt ggtagacctt atattttgaa tgttgatggt gcaatacacg
     5581 atggatggtt ggctatttcg aactatgaaa actcattaaa taaagattac ctattctata
     5641 ttctttcatc aaatgtagtt tattctcaat ttctatctct aattagtgga gctgttgtga
     5701 aaaacttgaa tagtgataaa gttgcttcta ttcttatc
//
LOCUS       DAVBCR010000100         4917 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCR010000100 DAVBCR010000000
VERSION     DAVBCR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.100"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            237..455
                     /locus_tag="VQ508_001909"
     CDS             237..455
                     /locus_tag="VQ508_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2490861.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1500..2492
                     /locus_tag="VQ508_001910"
     CDS             1500..2492
                     /locus_tag="VQ508_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2490862.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2494..3312
                     /locus_tag="VQ508_001911"
     CDS             2494..3312
                     /locus_tag="VQ508_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2490863.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3314..4099
                     /locus_tag="VQ508_001912"
     CDS             3314..4099
                     /locus_tag="VQ508_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490864.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4138..4416
                     /locus_tag="VQ508_001913"
     CDS             4138..4416
                     /locus_tag="VQ508_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2490865.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4401..4598
                     /locus_tag="VQ508_001914"
     CDS             4401..4598
                     /locus_tag="VQ508_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490866.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1413 a          809 c         1029 g         1666 t
ORIGIN      
        1 cgtcagttcc atctacaacc tcaaaacagt gttttgagca acctgaggct agtttcctag
       61 tttgctcttt ggttttcatt tagtataaca gattgttaga agttggttta aatttcctaa
      121 tcagtttgtt cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag
      181 atgattatag tcatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga
      241 aaaaaatatc tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg
      301 atattctata cgtaaatggt actgctattc tttatcatta tttacgttct attgtttcaa
      361 tagtttcggc aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt
      421 tactagataa atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt
      481 aaggaaaagt aaaatcagta agaaaaatgt cttgcattat acagcaacct tttgggaatg
      541 agtagatgga ttgaataaaa ttttattaag agtggatgat ttatctgtag attattattg
      601 gacagttagt cttgaagtag tctaagaatt aggttataat cagtagaatc cttgctaata
      661 atgaggaggt tagtttatgt atagtagact gaatctaaaa tagtacgaaa caattgctaa
      721 aacatttata gaaattaatt ttactttccc aatcgatttg ttctcatctt atttcaatcc
      781 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
      841 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      901 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      961 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
     1021 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
     1081 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
     1141 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
     1201 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
     1261 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
     1321 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
     1381 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
     1441 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
     1501 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
     1561 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
     1621 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
     1681 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
     1741 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1801 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1861 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1921 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1981 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     2041 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     2101 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     2161 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     2221 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     2281 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     2341 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     2401 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     2461 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     2521 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     2581 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     2641 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     2701 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     2761 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2821 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2881 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2941 actgtcattt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     3001 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     3061 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     3121 tcagatattc tctccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     3181 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     3241 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     3301 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     3361 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     3421 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     3481 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     3541 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggggagtt
     3601 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     3661 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     3721 cattgtttgg actcttccga aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3781 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3841 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3901 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3961 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     4021 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     4081 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     4141 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     4201 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     4261 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactagatt tgtaagctta
     4321 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     4381 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     4441 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     4501 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     4561 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     4621 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     4681 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     4741 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4801 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4861 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactctt
//
LOCUS       DAVBCR010000101         4548 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCR010000101 DAVBCR010000000
VERSION     DAVBCR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.101"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            157..1416
                     /locus_tag="VQ508_001915"
     CDS             157..1416
                     /locus_tag="VQ508_001915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2490867.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            1432..1983
                     /locus_tag="VQ508_001916"
     CDS             1432..1983
                     /locus_tag="VQ508_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490868.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            1976..3253
                     /locus_tag="VQ508_001917"
     CDS             1976..3253
                     /locus_tag="VQ508_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2490869.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3269..4129
                     /locus_tag="VQ508_001918"
     CDS             3269..4129
                     /locus_tag="VQ508_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2490870.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            4352..4462
                     /locus_tag="VQ508_001919"
                     /pseudo
     CDS             4352..4462
                     /locus_tag="VQ508_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         1368 a          731 c         1044 g         1405 t
ORIGIN      
        1 tctttgattt tcattgagta ttagtaattc agttactaac tcgtcaactc tgatttatcc
       61 aataaaattg aaaaggatgg aaaaaaggat aaatttatga tatactttat tttgaagacc
      121 ttattagaaa tcttgaaaga gtattgaaaa cttagaatga gaaaaattgt tatcaatggt
      181 ggattaccac tgcaaggtga aatcactatt agtggtgcta aaaatagtgt cgttgcctta
      241 attccagcta ttatcttggc tgatgatgtg gtgactttgg attgcgttcc agatatttcg
      301 gatgtagcca gtcttgtcga aatcatggaa ttgatgggag ctactgttaa gcgttatgac
      361 gatgtattgg agattgaccc aagaggtgtt caaaatattc caatgcctta tggtaaaatt
      421 aacagtcttc gtgcatctta ctatttttat gggagcctct taggccgttt tggtgaagcg
      481 acagttggtc taccgggagg atgtgatctt ggtcctcgtc cgattgactt acaccttaag
      541 gcgtttgaag ctatgggtgc cactgctagc tacgagggag ataacatgaa gttatctgct
      601 aaagatacag gacttcatgg tgcaagtatt tacatggata cggttagtgt gggagcaacg
      661 attaatacga tgattgctgc ggttaaagca aatggtcgta ctattattga aaatgcagcc
      721 cgtgaacctg agattattga tgtagctact ctcttgaata atatgggtgc ccatatccgt
      781 ggggcaggaa ctaatatcat cattattgat ggtgttgaaa gattacatgg gacacgtcat
      841 caggtgattc cagaccgtat tgaagctgga acatatatat ctttagctgc tgcagttggt
      901 aaaggaattc gtataaataa tgttctttac gaacacctgg aagggtttat tgctaagttg
      961 gaagaaatgg gagtgagaat gactgtatct gaggacagca tttttgtcga ggaacagtct
     1021 aatttgaaag caatcaatat taagacagct ccttacccag gctttgcaac tgatttgcaa
     1081 caaccgctta cccctctttt actaagagcg aatggtcgtg gtacaattgt cgatacgatt
     1141 tacgaaaaac gtgtaaatca tgtttttgaa ctagcaaaga tggatgcgga tatttcgaca
     1201 acaaatggtc atattttgta cacgggtgga cgtgatttac gtggggccag tgttaaagcg
     1261 accgacttaa gagctggggc tgcactagtc attgctgggc ttatggctga aggtaaaact
     1321 gaaattacca atatcgagtt tatcttacgt ggttattctg atattatcga aaaattacgt
     1381 aatttaggag cggatattag acttgttgag gattaaaccg tagaggtgtt tatgaatatt
     1441 tggaccaaat tagcaatgtt ttcttttttt gaaacggatc gcttgtattt gcgtcctttc
     1501 ttttttagtg atagtcagga cttccgcgag atagcttcaa atccagaaaa tcttcaattt
     1561 attttcccaa cgcaggcaag tctggaagaa agtcaatatg cactggccaa ttactttatg
     1621 aagtcccctt tgggagtgtg ggcaatttgt gaccagaaaa atcaacaaat gattggttct
     1681 attaaatttg agaagttaga tgaaatcaaa aaagaagctg agcttggcta ttttttgaga
     1741 aaagatgctt ggtcgcaagg atttatgaca gaggttgtta gaaaaatttg tcagctttct
     1801 tttgaggaat ttggcttaaa acaattattt atcattaccc accttgaaaa tgaagctagc
     1861 caaagagttg ctcttaagtc tggatttagt ttgttccgtc agtttaaggg aagtgatcgt
     1921 tacacaagaa aaatgcggga ttatcttgaa tttcggtatg taaaaggaga gttcaatgag
     1981 taagcatcag gaaattctaa gctatttgga ggaattacca gtaggtaaaa gggtcagtgt
     2041 tcgtagcatt tcgaatcatc taggagttag tgatggaaca gcctatcgag ctattaaaga
     2101 agctgaaaac cgtggaattg tggagacccg tcctagaagt ggaacaattc gtgttaaatc
     2161 ccagaaagtt gctatagata gattaacgtt tgctgaaatt gcagaagtga cttcttctga
     2221 ggttctggct gggcaagaag gtttagagag agaatttagt aagttttcaa ttggtgccat
     2281 gactgaacaa aatatcttgt cttaccttca tgatgggggg ctcttgattg tcggagaccg
     2341 aacccgtatt cagttgctag ccttggaaaa tgaaaatgca gttctggtta cagggggatt
     2401 tcaggttcat gatgatgtgc ttaaactggc caatcaaaaa gggattcctg ttctaagaag
     2461 taagcatgat acctttaccg tcgctaccat gatcaataaa gccttgtcaa atgtccaaat
     2521 caagactgat attctgacag ttgagaaact ttatcgccct agtcatgagt atggttttct
     2581 gagagagaca gatacagtta aagattattt ggacttggtt cgtaagaatc gtagcagccg
     2641 tttccctgtt atcaatcaac atcaggtcgt tgttggcgtt gtaaccatga gagacgctgg
     2701 tgataaatca ccaagcacga caattgataa ggttatgtct cgtagtctat ttttggttgg
     2761 attatcgaca aatattgcca atgtgagtca acggatgatc gcagaagact ttgaaatggt
     2821 accagttgtt cgaagcaatc aaactttgct tggcgttgtg acgcgacgag atgtcatgga
     2881 gaagatgagc cgttcccaag tttcggctct accaactttt tctgagcaga ttggacaaaa
     2941 gctctcttat caccatgatg aagtagtcat tacagtggaa ccctttatgc tagaaaaaaa
     3001 tggagttttg gctaatggtg tattggcaga aattctgacc cacatgaccc aagatttagt
     3061 tgttaatagt ggtcgcaatc tcattatcga gcagatgctg atctactttt tgcaggccgt
     3121 tcagatagat gatatattgc gcattcaggc acggattatt catcatacga gacggtcagc
     3181 tataattgat tacgatattt atcatggtca ccagattgtt tcaaaagcaa atgtgactgt
     3241 taaaattaat tagaaactag gagaaaagat gataacatta aaatcagctc gtgaaatcga
     3301 agctatggac aaggctggtg attttctagc aagtattcat ataggcttac gtgatttgat
     3361 taagccaggc gtagatatgt gggaagttga agaatatgtc cgccgtcgtt gtaaagaaga
     3421 aaatttcctt ccacttcaga ttggggttga cggtgccatg atggactatc cttatgctac
     3481 ctgttgctct cttaacgatg aagtggctca cgctttccct cgtcattata tcttgaaaga
     3541 tggtgatttg ctcaaagttg atatggtttt gggaggtccc attgctaaat ctgacctaaa
     3601 tgtctcaaaa ttaaacttca acaatgttga acaaatgaaa aaatacactc agagttattc
     3661 tggtggttta gcagactcat gttgggctta tgctgttggt acaccgtccg aagaagtcaa
     3721 aaacttgatg gatgtaacca aagaagctat gtacaagggt attgagcaag ctgttgttgg
     3781 aaatcgtatc ggtgatatcg gtgcggctat tcaagaatac gctgaaagtc gtggttacgg
     3841 tgtagtgcgt gatttggttg gtcatggtgt tggcccaact atgcacgaag aaccaatggt
     3901 tcctaactat ggtattgcag gtcgtggact ccgtcttcgt gaaggaatgg tcttaaccat
     3961 tgaaccaatg atcaatacag gcgattggga aattgataca gatatgaaaa ctggttgggc
     4021 gcataagacc attgacggtg gattgtcatg tcagtatgaa caccaatttg tcattacgaa
     4081 agatggacct gttatcttga ctagccaagg tgaagaagga acttattaat aaaaagtgaa
     4141 aagactactg gaagtttatt atgaaaaaat ccagtagatc ttttcataat aaaacgcatt
     4201 gtatcaagtg ttaggggctg atatcatgcg tttttctgct tttaagattt tttccaactc
     4261 tgtttgtaag cgcatcataa caaagggtct aggattcagg gctctcctcc tatatactat
     4321 tagtaaagta aaactaaggg aggatatttt agtgtcgcag tctattgttc ctgtagagat
     4381 tccacaatat tgtcgttttg attctaaaaa gagaaatgga attctgttta atgttcgtat
     4441 tgccaatctt aaatttacct tttttagatt agacttcctg cgaaacaaaa catggtatag
     4501 tagttctatg aatgatgaag caagtaaaca actaactgat gcacgatt
//
LOCUS       DAVBCR010000102         4461 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCR010000102 DAVBCR010000000
VERSION     DAVBCR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.102"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(32..265)
                     /locus_tag="VQ508_001920"
     CDS             complement(32..265)
                     /locus_tag="VQ508_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490871.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(258..374)
                     /locus_tag="VQ508_001921"
     CDS             complement(258..374)
                     /locus_tag="VQ508_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2490872.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(589..942)
                     /locus_tag="VQ508_001922"
     CDS             complement(589..942)
                     /locus_tag="VQ508_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2490873.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            1195..3828
                     /gene="polA"
                     /locus_tag="VQ508_001923"
     CDS             1195..3828
                     /gene="polA"
                     /locus_tag="VQ508_001923"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2490874.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3913..4350
                     /locus_tag="VQ508_001924"
     CDS             3913..4350
                     /locus_tag="VQ508_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2490875.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1211 a          823 c         1080 g         1347 t
ORIGIN      
        1 atttacccac tatggatatt atggagccat tttacaacag tgcctcaaat tcagatactg
       61 acatacctaa ttgctggctg gcaacctcag aagtcagaag accttggcgt actatgtcta
      121 ggaaggcaaa aagtttcctt tctgcccttc cttcaacttt cccacgctct agtccttgtt
      181 caaggccttc agccctagca gtttccaagg catagtcctg tgctaacaag acttgttctt
      241 ctcgcatatg tagttgacta aacattttcc tgtcctcctc ggaccagctc ttgtagtcca
      301 gcagttgatt tgcttgggta atggctcgtt gcggttgctg ggtaaagggc ttgttgccga
      361 aaaactccaa ccatggcttg cgaaccttgt ctttgctggt ttctctgtat tttttaattc
      421 caagaatgcc atcttaacca gatggttttc ctgtccattg ttggtaatcg ccaatacctc
      481 acctgttgtg tcttcgcgca tactaaagct atgaaaagcc aggtcatctg agaaataatt
      541 actatccacg atagcaatga cgtaaacagg agcgatgtgt ttgtagatct agtgagtatc
      601 accttctcgc tgacgaattt tttcaagatt ttgattaacc tgactgcaca ggtaagccca
      661 caagtgattg atgaaaaaat tctgatgatg gacttgaatc tcaataatta cttgagtacc
      721 gttatccaac tccgccaaga cgtctatact ggtataaaaa tcctgcgctg agtagggcat
      781 ggaaggcaag acatgaatgt tacttccctc caaaatagtc acatttttgg ctggcaagtc
      841 cagcatatcg cggataaatt gacaagtgat ttctgggttg ctgaaaattt tcttagcaac
      901 caagtcattg gtcgggctaa ttcccagatg tctgagaatc atccttttcc tccttctata
      961 gtgaattgaa ataagatgtg aacaactcta tcaggaaagt caaattaatt tatagaaata
     1021 ttttagcagt caaggtgtac tattatagat tcaatatact atattatagc acatttttaa
     1081 atctaggttt actaaattct ctggctctat ctatttattc ggaaaaagtt tgaaaaatac
     1141 ttgctctagc tcttcccaat ggtatttttt gattctttcc tttataatgg gtgtatggat
     1201 aagaaaaaat tattattgat tgatgggtct tctgtagctt ttcgggcgtt ttttgcgctg
     1261 tatcagcagt tggaccgttt taagaatgcg gctggtttgc ataccaatgc gatttatggt
     1321 tttcagttga tgttgagtca tttgttggag cgggttgagc cgagtcatat tttggtggct
     1381 tttgatgcgg gaaagacgac cttccggaca gagatgtatg cggactataa gggtggtcgg
     1441 gccaagactc ctgatgagtt tcgtgagcaa tttcctttca ttcgtgagtt gctggatcat
     1501 atggggattc gtcactatga gctggctcag tatgaggcgg atgacatcat tgggacgttg
     1561 gataagctag cagagcagga tggttttgat attaccattg tcagtgggga caaggatttg
     1621 attcagctga cggatgagca tacggtggtt gaaatttcca agaaaggtgt ggctgagttt
     1681 gaggccttta cgccagatta cctcatggaa gaaatgggcc tcacaccagc tcagtttatc
     1741 gatctcaagg cgctcatggg tgataagtcg gataatatcc ctggggtgac caaagtcggt
     1801 gaaaagacgg gtattaagct cttgctggag catggttcgc ttgaggggat ttatgaaaat
     1861 attgatggaa tgaagacttc taagatgaag gaaaatctca tcaatgacaa ggaacaggcc
     1921 tttttgtcta aaacactagc gaccattgat accaaggcac cgattgcgat tggtttagag
     1981 gacttggtct atagtggtcc agatgttgaa aatcttggga aattttacga tgagatggga
     2041 ttcaaacagc tcaagcaggc tttaaatgtg tcgtcagctg atgtggctga gggtttggat
     2101 tttactattg ttgaccaaat cagtcaagat atgctgagtg aagagtctat cttccacttt
     2161 gagctttttg gtgagaatta tcatacggat aatttggttg gatttgcctg gtcttgtggg
     2221 gataagctct atgccacaga caagcttgag ctgttgcaag acccgatttt caaggatttc
     2281 ttagaaaaaa catctctgag agtttatgac tttaagaagg ttaaagttct tttgcaacgt
     2341 tttggtgtgg atttgcaggc gcctgctttt gacatccgtt tggctaaata cctcctttcg
     2401 actgtggagg acaatgaaat tgcgaccatc gctagtcttt atggtcagac ttacttggtt
     2461 gatgatgaaa ctttctacgg taagggtgtt aaaaaggcca ttcctgaacg tgagaaattc
     2521 ttggaacact tagcttgtaa acttgctgtt ttggtagaaa cagagcctat tttacttgaa
     2581 aaactcagcg aaaatgggca attagagctt ctttatgata tggagcaacc tctggctttt
     2641 gttcttgcca agatggaaat tgctgggatt gtggtcaaga aagagacctt gcttgagatg
     2701 caggctgaaa atgagcttgt cattgaaaaa ctgactcaag agatttacga gctggctggt
     2761 gaggagttta atgtcaactc gcctaagcag ttgggcgtgc ttctctttga gaaattggga
     2821 cttcctctag aatacactaa gaaaaccaag acaggttatt cgacagcagt ggatgtgttg
     2881 gagcgtctcg ctcctattgc tccgattgtt aagaaaatcc tggattaccg tcaaattgct
     2941 aagattcaat ctacttatgt aattggcttg caggactgga ttttggctga tggaaagatt
     3001 catactcgct atatgcagga tttgacccag accgggcgtt tgtctagtgt ggatccaaac
     3061 ttgcaaaata ttcctgcccg attggaacaa gggcgcttga ttcggaaggc ttttgtgcca
     3121 gagtgggagg atagtgtgct actcagctct gactattcac agattgaatt gcgcgttttg
     3181 gcgcatattt ctaaggatga gcacttgatt aaggccttcc aagagggggc agatatccat
     3241 acttcgacag ccatgcgggt ctttggcatt gagcgtcctg ataatgtgac tgcaaatgat
     3301 cgtcgcaatg ccaaggcagt taactttgga gtggtttatg ggatttcaga ctttggcttg
     3361 tctaataatt tgggaattag tcgtaaggaa gccaaagcct acattgatac ctactttgaa
     3421 cgttttccag gtattaaaaa ctacatggat gaagtggtgc gggaggcgcg tgataagggc
     3481 tatgtagaga ccctctttaa gcgtcgccgt gagttgccag atatcaattc gcgcaacttc
     3541 aatattcgtg gttttgcgga gcgaactgct atcaactcac ctatccaggg ttcggcagca
     3601 gatattctca agattgccat gattcagctg gataaagcct tggttgcagg tggttatcag
     3661 actaagatgc tgttacaagt gcacgatgaa atcgtccttg aagtgcctaa atctgaattg
     3721 gtagagatga aaaaattggt gaaacaaacc atggaagaag ccattcaact cagtgttcct
     3781 cttatcgcag atgagaatga aggggcaacc tggtacgagg ctaaataaaa agggggctag
     3841 tcctcctttt ttgtagtaga attctgcaaa ccttttcaaa atgtgctata ctgatgaaaa
     3901 aggaggtttt ctatgagtca agaatttatc aatccaagtg atggcgtgat tcgtcagtat
     3961 ctcgcaacga gtaaaaccct tgctgtggtg ggtttgtctg accgtgaaga aacaaccagc
     4021 aatcgagtga caaaggaaat gcaggctcgg ggctataaaa tcatcccagt caatcccaag
     4081 gcggcaggtg gcgaaatctt gggtgaaaag gcttatgcta gcctcgctga aattcctttt
     4141 cctgtagata ttgtcaatgt ttatcgtcgc agtgaatttc tgcccgatgt ggcgcgtgat
     4201 tttctcaaag ctgatgccaa gattttttgg gcacagctag gacttgaaag tttagaagcg
     4261 aaagagattt tgcgtgatgg tggatgtgat gatatcgtga tgaatcgctg tatcaagaga
     4321 gaacatactc gcttgattga ggaagcataa gaaaaaggta gctgatgggc taccttttgt
     4381 gttatactca atgaaaatca aagagcaaac taggaaacta gtcgcgggct gctcaaaaca
     4441 ctgttttgag gttgtagata c
//
LOCUS       DAVBCR010000103         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCR010000103 DAVBCR010000000
VERSION     DAVBCR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.103"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ508_001925"
     CDS             <1..119
                     /locus_tag="VQ508_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2490876.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ508_001926"
     CDS             121..966
                     /locus_tag="VQ508_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2490877.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ508_001927"
     CDS             976..2295
                     /locus_tag="VQ508_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2490878.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQ508_001928"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ508_001928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ508_001929"
     CDS             complement(2839..4110)
                     /locus_tag="VQ508_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2490879.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCR010000104         4423 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCR010000104 DAVBCR010000000
VERSION     DAVBCR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.104"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQ508_001930"
     CDS             complement(56..1066)
                     /locus_tag="VQ508_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2490880.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQ508_001931"
     CDS             1215..2384
                     /locus_tag="VQ508_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2490881.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQ508_001932"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQ508_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2490882.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQ508_001933"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQ508_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2490883.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQ508_001934"
     CDS             4146..4379
                     /locus_tag="VQ508_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2490884.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1286 a          842 c         1135 g         1160 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aat
//
LOCUS       DAVBCR010000105         4334 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCR010000105 DAVBCR010000000
VERSION     DAVBCR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.105"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..141
                     /locus_tag="VQ508_001935"
     CDS             <1..141
                     /locus_tag="VQ508_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490885.1"
                     /translation="LLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            complement(134..307)
                     /locus_tag="VQ508_001936"
     CDS             complement(134..307)
                     /locus_tag="VQ508_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2490886.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(465..2072)
                     /locus_tag="VQ508_001937"
     CDS             complement(465..2072)
                     /locus_tag="VQ508_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2490887.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2225..3709
                     /locus_tag="VQ508_001938"
     CDS             2225..3709
                     /locus_tag="VQ508_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2490888.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3804..4286
                     /locus_tag="VQ508_001939"
     CDS             3804..4286
                     /locus_tag="VQ508_001939"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2490889.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1254 a         1002 c          821 g         1257 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
      121 tcttgttctt gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg
      181 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      241 aagcagctaa aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta
      301 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      361 aaagtttata aaccttggtt ataaaaaaca aaggatttct gcttttatac agtcctccct
      421 tgtttttata tgatttcaat tttaaatttt tctgcaaaaa atatttatag taattccaca
      481 cagaaagcat cccatggaac taagatttgt ttttcaaaga cttcttgagc tagggtgttt
      541 tcaatcaaga cagatttgac ttttccttct actgtcaagt cttgctcttc attggacaag
      601 ttagccacaa ctaggaagcg acggtcgcca tccttacgta tataagcaaa gaccttatca
      661 gccgtatcaa gcaattcaaa gtcagctcga actagccagc tattctcctt gcgaatttgg
      721 accagtttct gataggtata gaaaatagaa tctggatttg ccagcgcttc ttggacattg
      781 atcatctcgt aatttggatt aaccgccaac caaggttgac ctgttgagaa accagcgttt
      841 ttgctctcgt cccattgcat aggggtacgg gcattgtcac gtccaataac acggatactg
      901 tccatgattt cttcaatcgg aacacctttt tcaagagcct cacgcgcata gttgagagat
      961 tcaatatctt ctacttgatc cagtgtttca aacggatagt tggtcatccc aatctcctca
     1021 ccttggtaga tataaggagt tcctctcata agatgaagca agattgcaaa ggctttggca
     1081 gatttttcgc ggtattcttg gtcatttccc cagattgaga caatacgagg gaggtcatgg
     1141 ttgttccaga agagggaatt ccagccgtcc tcaactccta actctgtctg ccatttgttg
     1201 aagatttctt ttaacttagc gatattcagc tctttttgat agtgccattt aggctgacct
     1261 tcctgatact gaagaccgat atgttcaaac tggaagacca tagacaattc ttgccccttt
     1321 ggatcagagt agagcttggc aatctctggc gttgctcccc aagtctcccc tactgtcaag
     1381 agatccttat ctccaaaggt cgcctgattc atttccttga gatagggatg gagcatagga
     1441 ccattattga ctaccttctc gtcaggaatt ttgccaatca tgtcaataac atccatacgg
     1501 aaaccaccaa tacccttatc aatccagaag ttcatcatct cataaatttt ctggcgaagt
     1561 ttttcatttt cccagttgag atccggctgt ttcttgctga aaaagtggag atagtattga
     1621 cctgactttt catcgtattc ccaagcagac ccactaaaga tagaatctaa atcgttaggc
     1681 tcatcccgcc agatatagta gtctcgctca gggctattag gattttcaca ggcctctaca
     1741 aaccaggcat gctcatccga ggtatgattg accaccaagt ccatgatgat acggatatca
     1801 cgcttcttag cttctgcaat cagctgatcc atgtcctcca tggttccaaa aatagccgca
     1861 atcgcttgat aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga
     1921 gaaagccaaa ttgctgtgat tcctagcttg gctagatagt ccaacttact ggtaattcct
     1981 ggcaaatcgc caattccatc tccattacta tccataaaac tctttggata gacttgatag
     2041 actacggcat tatgccacca tttttcttgc atctgtttac ctcattattt tcataatcta
     2101 tagtctatga tagcactttt taaaattttg tgcaagcgtt tgcttaatct ttttaatttc
     2161 ttttttagct ccgtagtttc ctaacttcca attttttatt ataatagagt ccagaggtaa
     2221 aaaaatgaaa aaacaagctt ttagttctga acagtatttg aatttacaac gcgaccatat
     2281 tttggagcgc attaaccaat ttgacggcaa gctctactta gagtttggcg gtaaaatgtt
     2341 agaagatttc cacgctgctc gtgtccttcc tggttatgaa cctgacaaca aaatcaagct
     2401 cttgcaagaa ttgaaagagc aggttgaggt tgtgattgcc attaatgcta gcaacattga
     2461 acattccaaa gcacgtggcg acttgggcat ttcttatgac caagaagttc ttcgtttgat
     2521 tgacaaattc aatgaattag ggatttttgt tggttccgtt gtcattacac aatacgctgg
     2581 ccaacccgct gcagatgcct tcctcaacca acttgagaaa aacggaattg attcttatct
     2641 tcattatcca atcaaaggat atccgacgga tatggatcac atcatttccc cagaaggcat
     2701 gggcaaaaac gactacatca aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc
     2761 cggttctgga aaattggcaa cgtgtatgtc caatatgtac cacgaccaaa tcaatggcat
     2821 caagtctggc tacgctaaat ttgaaacctt ccctgtttgg aatcttcccc ttcatcatcc
     2881 agttaacttg gcttatgaag ctgccacagc tgaccttgat gatgtcaaca tgattgaccc
     2941 cttccatctt caaacctatg gagaaaccac tgtcaactac aaccgtgata tcgaaatctt
     3001 cccagtgctc aagcgcatgt tggaacgtat tctcggaaaa tcaccatacg cttcaccgac
     3061 agatatgggt gtcaacatgg ttggtttcgc tattacagat gacgaggctg ctgtcgaagc
     3121 ttctaaacaa gaaatcatcc gccgttacta tcaaacagtt cttgacttca aagctgaaaa
     3181 agttggcgaa gctgccgtca agaaaattga gttgctcatg aacgacctcg gtatctcacc
     3241 tgcagaccgt aaggttgctg tcgttgcgcg ccaaaaagca gaagaaactg gcggaccagc
     3301 cctagctctt gaattaccaa atggtgacat tgtcactggt aaaaactcag aactctttgg
     3361 ccctacagcc gctgccttga tcaacgccat caaaaaatca gctgacatcg ctaaagaagt
     3421 aaaactaatc gagcctgaag ttgttaagcc aatccaagga cttaaaatcg atcatctcgg
     3481 tagccgcaat ccacgccttc attcaaatga aatcctgatt gcacttgcta tcacagctac
     3541 agaaaatcct gatgctgccc gcgctatgga agaactcggc aacctcaaag gaagcgaagc
     3601 ccactcaacc atcatcttga ctgatgaaga caagaatgtc cttcgtaaac tgggtatcaa
     3661 cgtaaccttt gacccatact accaatacga ccgcttatat cgtaagtaaa gatttcaacc
     3721 tctccagcct cggagaggtt ttctctctgt ctcaggagca agctttatga tataatgaga
     3781 gaagaaaact gaaaggattt accatgtcaa aagaagttat tgtcgaaagt tttgaacttg
     3841 accacaccat tgtcaaagca ccctatgttc gcttgattgg ggaagaaaca ggaccaaaag
     3901 gagacatcat ctccaattat gatattcgct tggtgcaacc aaacgaagac tctatcccta
     3961 ctgccggcct tcacactatc gagcacctct tagccaaact catccgtacc cgcattgacg
     4021 gcatgattga ctgttcacca tttggttgcc gcacaggctt ccacatgatt atgtggggac
     4081 gtcacaccag tgctaaaatc gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa
     4141 ctactacttg ggaagatgtc ccagggacaa ccatcgaatc ttgcggaaac tacaaggacc
     4201 acagcctctt ctctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg
     4261 atgcctttga acgtcatgtg atttaaaatc gagtagggga taatctctag cccctctcac
     4321 accaccgtac gtgc
//
LOCUS       DAVBCR010000106         4289 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCR010000106 DAVBCR010000000
VERSION     DAVBCR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.106"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(105..1301)
                     /gene="tuf"
                     /locus_tag="VQ508_001940"
     CDS             complement(105..1301)
                     /gene="tuf"
                     /locus_tag="VQ508_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2490890.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2212..3081)
                     /locus_tag="VQ508_001941"
     CDS             complement(2212..3081)
                     /locus_tag="VQ508_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2490891.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3304..3927)
                     /locus_tag="VQ508_001942"
     CDS             complement(3304..3927)
                     /locus_tag="VQ508_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2490892.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKAETPVNPDPE
                     VPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(3885..>4289)
                     /locus_tag="VQ508_001943"
     CDS             complement(3885..>4289)
                     /locus_tag="VQ508_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2490893.1"
                     /translation="TFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYK
                     EGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANA
                     YQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT         1202 a          874 c          847 g         1366 t
ORIGIN      
        1 gattttcatt gagtattagt tcaatagcta aaaaaagaac cttgccaagc aagattcttt
       61 tagtgtctga cttaaataat tgttcttctg ggaactaaat cgaattaagc ttcgatttct
      121 gtaaccatac ctgaaccaac agtacgtcca ccctcacgga tagagaatgt agtaccttgt
      181 tctacggcga ttgggtgaat caactcaaca tcgattgtca cgttatcacc aggcattacc
      241 atttcagtac ctgctggaag ttcgattgaa cctgtaacgt cagtagtacg gaagtagaat
      301 tgtggacggt agttgttgaa gaatggagtg tgacgtccac cttcttcttt agtaaggatg
      361 tagacttcac ctttgaattt agtgtgtggg ttgattgaac ctggtttagc gataacttgt
      421 ccacgttcga tttcatcacg ttgaacacca cgaagaagga cacctacgtt atctccagca
      481 agaccttcgt caagttgttt acggaacatt tcaacaccag taacaactgc tttttgagtt
      541 tcttctttga taccaacgat ttcgatttcg tcgttgactt taacgatacc acggtcgata
      601 cgtcctgaag caactgtacc acgtccagtg attgagaata cgtcctcgac tggaagaagc
      661 aatggtttgt cagtgtcacg ttctggttct gggatatact catcaactgt gttcatcaat
      721 tccataacga tgtcttcgta tttagagtca ccttcaagag ctttaagtgc tgaaccttgg
      781 ataactggaa gatcgtcacc tgggaagtcg tattctgaca ataggtcacg gatttccatt
      841 tcaaccaatt caagcaattc ttcgtcgtca accaagtcaa ctttgttcat gaagacgata
      901 aggtgtttaa caccaacctg acgtgaaaga aggatgtgct cacgagtttg tggcattggt
      961 ccgtcagttg aagctactac aaggatagct ccgtccattt gagcagcacc agtgatcatg
     1021 tttttaacgt agtccgcgtg tcctggagcg tcgatgtgag cgtagtgacg tttttcagtt
     1081 tcgtactcaa cgtgcgcagt gttgatagtg ataccgcgtt cgcgttcttc tggagcagca
     1141 tcgatagacg catagtcttt aggttggtta actgatgaag gcaagcgacg tgccaaaaca
     1201 gttgtgatag ctgcagttag ggtagtttta ccgtggtcaa cgtgtccgat agtaccaatg
     1261 ttaacgtgtg gtttactacg atcgtatttt tcttttgcca tttgagtaaa agcctccaat
     1321 aaaatatatt ttatagatag acagtaggca atacagtcta actttcctta ctattttatc
     1381 aaatttcagc taaattgcaa gtgttttaca acgtttttgt gaattattct tggtaaaaat
     1441 ttaactaatt aatagaaagt actaaataga cttatccttc aaggagtttt cagcattatc
     1501 catccactca cttgctgaaa atgtaaacag tctctggttt atatagtaaa atgaaacaag
     1561 aatagagcaa atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagatg
     1621 tgtactattc tagtttcaat ctactataca acctcaaaac tgtgttttga acaacctgcg
     1681 gctagcttct tagtttgccc tttgattttc attgagtata aaattgcaga taaaacgata
     1741 acaatcatag gactcagcag agtcaatgta ggtcgaagaa tagcccccct aatcgcttat
     1801 tttatagcaa aatgaaatag aaactgaaca ttttgttgag gaaggtaaat caattcctca
     1861 caatgtttga gaaagaaggg aggactattc caacttcaac ccactataaa gcagacttac
     1921 aagataggga acccatctcc gataatatat agtagaatga aaactgcaca atttgattgg
     1981 agaattcaaa ccaatttcta acaatgtttt agaaacaagg gtctattatt tcaactgcaa
     2041 cctactatag cccattgaag ctggaatagc ccactgagac ttctaaacca ctgctggaga
     2101 ggagtttgtt tagagtttct ttcatttccc taaaaaatca aaaagagaga accagtctga
     2161 tcctctctta cttatagatg gtgctcactc aaatgttaaa ggagctattt cttagagata
     2221 aaggaatttg aatacagcta ctgccgcaat tgctgctgcg ataggtgcta ctactggtac
     2281 ccaagaatac caccattttg aatcgccttt atgctcacca agaactgatt ttggaaggaa
     2341 agcatgaagg agacgtggtc ccaagtcacg ggctgggttc aaggcaggtc ctgtaggtcc
     2401 tccaagtgat gttaccaaag ccataacgag gaatccaagt gccaagtgag ccacagaaag
     2461 tcctgaagca gtgtgtggag ccacctgtgc tttaatagcc aagtcagaaa aatcaactgt
     2521 ttgtcctgct tctattgcct tttgtttcat gaattgaagc acttcagcac caaagaagtt
     2581 tttagtcaaa ccaagagctg caaagaacaa aacaaatgaa ccaacaaact cattaatcaa
     2641 accattgaca gttgctgcat agcgactttc ttttgtacca tggtcaatac ttgaaatagt
     2701 tgagaaagtt cccaagatgt tatttggttt ttcagttttc aagtagaatg gacggtatgt
     2761 tgccacaact aaggcttggc caaagatagc ccccaagact tgcgcgataa tgtaaggtac
     2821 cacttgtgcc caagggaaaa gaccgctaac tgcaagccct agagtgaaag cagggttgat
     2881 gtgattccca gatacgttac caaacatcaa ggctgggatc ataaccccca taccataacc
     2941 aacagcgatg acgatccagc cactttggtg acctttcgta cctttaagtt caacgttggc
     3001 aactgcacca ttcccaagaa tgatcaaaat ggcagttccc aaaaattcag tggcatactt
     3061 cagtgcccat gtgaaatcca tttgctagat tctccttaaa attttttaga tattccttat
     3121 tttatcaatt tttaccctat ttagcaactg atttctggaa atatttttaa aaaaatagga
     3181 ttcatagttt cccagttcaa cgtacttgta agcgttttta ttcttcacga aagacctcat
     3241 tctcaaaacc aggaaaaagc ccacatctgt gatcttttcc tggttcctag attctaaaat
     3301 ctattatttt tcatcttctt ttttcttgaa gaatcctaat cctagaccag ctaacaaggt
     3361 catgatacca gcactagcca aggtagcatt agcttctgta cctgtgtttg gcaattcctc
     3421 tctcttacct gtctcataag tcggaacttc tgggtctgga ttcactggag tttcagcttt
     3481 tggagtacct ggttctggag ttggtttatc tggtgttgga gtctcttgat ttggtagttc
     3541 tgggtttggt gtcggaacac ttggatgctc tggatttggt tgtgggattg gtttttctgg
     3601 agttggttta tctggtgttg ataaacggtc ataccttacc gttatttctt tatcactaga
     3661 gtctgacgta acttcttgtg attcaactgt tggaatatct ggatctttgt acttgtcaat
     3721 cttaccagat ataacctcgt cccagtttcc tgttgtccat tcaccgtagg ttacaactcc
     3781 cgtgaccttg ttctcagttt ttgtacggct taaggttaca ggttgaacaa catcttcttt
     3841 tacattttgg ttcgtaactt tatcaacgta atgaatgata cgcgttatag tcttcgtctc
     3901 agtagaggtt gctgttttgg gaaccactgt ttcctcaaca ttctcacggt agtaatagtc
     3961 aactgttgca ccgtcttctg gtacgcattt gcaggagttg ctaccaaggt gtatgttgtt
     4021 ttccttgtga taactcggtc ttctttgtcc tcagttgttg ttttcccttc aatagttttt
     4081 gattctgtgg tatactcaga acctatcgct aaatcagctt ttataacaga ctctgccaac
     4141 ttctcttggc taccttcttt atagtaattg gctgttactg tagcagtggt tggcgcttcg
     4201 ctttctctat aaactaaggt cactgttcta ccttcgctta caatattccc agttaaactt
     4261 gcagaatttg tatctgcttc tttaaaagt
//
LOCUS       DAVBCR010000107         4172 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCR010000107 DAVBCR010000000
VERSION     DAVBCR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.107"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            52..693
                     /locus_tag="VQ508_001944"
     CDS             52..693
                     /locus_tag="VQ508_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2490894.1"
                     /translation="MRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(735..2723)
                     /locus_tag="VQ508_001945"
     CDS             complement(735..2723)
                     /locus_tag="VQ508_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490895.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2725..3483)
                     /locus_tag="VQ508_001946"
     CDS             complement(2725..3483)
                     /locus_tag="VQ508_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490896.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3911..4102
                     /locus_tag="VQ508_001947"
     CDS             3911..4102
                     /locus_tag="VQ508_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490897.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1274 a          825 c          810 g         1263 t
ORIGIN      
        1 tcagaaacaa atcacgaaat tgattcaaat tttgcaggtc gtttaaatat cctgcgtgcg
       61 ggtgttcttg atgctaacga tggaattatt tccattgctg gtgtggttat cggggttgcc
      121 agtgccacga ccaatatctg gattatcttt ttatcaggat ttgcggctat cttagctggt
      181 gccttttcaa tggctggtgg agaatatgta tccgtttcaa ctccaaaaga taccgaggaa
      241 gctgccgttt cgcgagaaaa actcttgcta gaccaagata gggaactagc caaaaaatcc
      301 ctctatgctg cttatatcca aaatgaagaa ttcaaaactt ctgcccaact cttgactaat
      361 aagatctttc ttaaaaatcc actcaaggct ctggtagagg aaaaatatgg gattgagtat
      421 gaagaattta ccaatccttg gcacgctgcc atttctagct tcgttgcctt tttccttaga
      481 agtttgcctc caatgctgtc agtgaccatt tttccaagtg attaccgcat ccctgctacc
      541 gtccttattg tcggtgtggc ccttcttctc actggttaca caagtgctag acttggaaaa
      601 gccccaacca aaacagctat gattcggaac cttgctattg gtctcttgac catgggagtt
      661 accttcctgc tcgaacaact tttcagcatt tagaatacaa gaaatacctc gattttgaag
      721 tcgaggtatc ttttttacat ttgcacaatc ttgcgataac ttcttgaagt aatcatgaaa
      781 atcagcacat aggcgatgag gaagatagcg cagatagaca aggtcacaat caacatcata
      841 gtcgtatcca gtacaccaat cacttttaaa atcagactaa gcatatggta ggcaaaggct
      901 agatgtagga aggcaaagag caaaggaagg aagaatacag ttaaaacctg tttgttgatg
      961 gtttgcttga tttgcttttg atctaaaccg actttctgca agataataaa gcgctcacgg
     1021 tcttcatatc cttcagaaat ttgtttgtag tagatgacca gaacggttcc gaccataaag
     1081 ataatggata agaaaatacc gataaagaag acaccaccaa agagggcact catctgagca
     1141 ctagcatctg ctagagtgct accatacaca tagttacctt cagtgtttaa ttgagcatta
     1201 aacttttgta agtatttttc atactcctca gcgaccttga gttgttctgc ttcactggca
     1261 tttacattca taccaccgta aaactgatta tagatagccg aatctgggaa ttgatccaaa
     1321 aaggcttgta aatcaggtac aacaaggtaa ttgtaatcag cagtcaaaat attaaactga
     1381 tttggaacat ggttgacaat aaaatcttta gtaaattctt cttttacaga aaattgatga
     1441 tcatttagag ttagagcttt ctgttcttta actccctcat tctttgcaaa gagtccgacc
     1501 tcatttcctg atagagacag tttttgacca gtcatatttt cataatcttt ttggtcaaat
     1561 accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt tttcaaaaat agttaactta
     1621 gttccttctt gatttgcaat accaaagtta ctgtaacgaa gtacttcttt ctctttgaca
     1681 ctataacctt tgtcacttgc aaactggctc aagagtttgt ccaagtcttc tttttcaaca
     1741 ttttgccctg aaaccccaaa atcatgagga tttagaactt ttttaaagct ttctgaggaa
     1801 ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca ttgttgacaa gatagcgatg
     1861 gtggctagtc caactgcatt tttcttcata cggaaaatca agttagaaac agatatgaga
     1921 ttatttggtt ggtaatagta tttcttattt ttctttaaga tttggaggaa aacggtaatc
     1981 cctgcattaa acaagagata agtcccaaag ataaccagta aaacagctat gaagaaggtt
     2041 gttaaggctg taagaggatc ttttaccgta agggcaagat aatagccaat tcctaaactt
     2101 atggaaccaa gaatggtttg gagaggaagg aagcgacctt ttttctctcc actagctttc
     2161 tcacgagaga gctggagggc attcatacgg gcgattcgaa gggcattcag gaacatgagg
     2221 cctaggaaaa tcaaaccgaa gacaacaagc actgtaatga caactttcgt ctggaaggta
     2281 gcaaccagct caaccttcaa tttcattagt ttgagcagga aagcgaaaat taacttgtca
     2341 aacaaggctc caataccgat acccgctcca acagttagaa tcccaaatac cactaactcc
     2401 ttaaaggtca tactgataag atgacgcttc tccaagccca acatgccata aattcctagt
     2461 tccttggaac ggttcttcat gacaaaacta ttggcataga gaacgataat ggctgacgca
     2521 agggtgacga caaacatacc aaatccaagt gtagcctgaa tggttgttcc tccacgaatt
     2581 tcagcaatct tgggattgaa ggttagagag taaaagagat aggtgacagt gactgccaag
     2641 agaacagcca gcgcaaaagg atagtagagt ttgcggtttt taatcaagtt cgataccgct
     2701 aacttattgg ttaatcgaaa catactaatt cacctcgctt gccatgacag tcaaggtatc
     2761 agagatttct tggaacatct gacgttctgt cttctctcca cggtagattt gattgtaaag
     2821 aatgccgtct ttgataaaga gtacacgttt agctctgcta gctgctgctg ttgagtgggt
     2881 taccatgaga atggtttggc cgcgctcatt gatttcatca aagacatcaa gtaaggctgc
     2941 agatgacttg gagtcaagag ctcctgtcgg ttcgtccgca aggagaattt caggttcagt
     3001 gatgatggcg cgtgctactg ctacacgctg tttctggcca ccagaaatct cgtaagggta
     3061 cttctcttgt aattggttta ttcccagatt ctcagctgtc accaccaatt tcttcatcat
     3121 ctctgtaata ggtcttcttg acaagacaag cggaagcaag atattgtcct ttacagatag
     3181 agtatctagc aagttaaagt cttggaagac aaagcccaac ttttcacgac ggaagctaga
     3241 ggcctgtgaa tttttaatgg ttgcggtgtc agttccattc aagtaaacct gaccacgact
     3301 tggtttatcc agcatagcta gaatattgag aagagttgat ttaccagaac cagactcacc
     3361 catgatggca acgtagtcac ccttttcgac ggtaaagtga atatccttga gggcttctac
     3421 ttggttgccc tgaaaacgtg ttttataaat tttttgaacg tgttttacat ctaaaagtgt
     3481 catgagaatc tcctttctta atatcccatc aaatgaaatg tagcttgcat catagtgcat
     3541 cccagctgaa cgcgctcaat ttctttgtga cttggttttc tagttttctt atgtaagatt
     3601 tgtttcatga tgttactcct ttgttcttta tgagtctagt ttacatcaaa aaaagaccgc
     3661 ttgccataac ctaaccttac aaaagagact ttaatcttac atttttgtaa gattgggtga
     3721 aatataagca aaattactta gaaaatgatt tgatttccta tttttaaagt tgtaaaatat
     3781 aagtatgaaa aggcttactt tggaaaggag gaagcgggaa ctatcatttg agtaagctta
     3841 cctagataac ataccattca atttaacact tgcaagtcgt tcaacctata aggaaagaag
     3901 gtgcgcttta atgaaacaca aagaacatat actcatcgga cttctctatc tgctctctcc
     3961 attcatcggt cagctcttag ttgaacacac acactttatc ggtacagaat ttaccggcac
     4021 tgcttatgtt atttgctggt tatcagttgt tatcagcatc caccattttt ccaaaaatgt
     4081 attgtctcag cagcaaaaat agattaaaaa actgagttta aattactgct tttataataa
     4141 aatgaaataa gaacaggaca aatcgatcag ga
//
LOCUS       DAVBCR010000108         4046 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCR010000108 DAVBCR010000000
VERSION     DAVBCR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.108"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..370)
                     /locus_tag="VQ508_001948"
     CDS             complement(<1..370)
                     /locus_tag="VQ508_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490898.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSG"
     gene            complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ508_001949"
                     /pseudo
     CDS             complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ508_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ508_001950"
     CDS             complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ508_001950"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2490899.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2422..3018)
                     /locus_tag="VQ508_001951"
     CDS             complement(2422..3018)
                     /locus_tag="VQ508_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2490900.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ508_001952"
     CDS             complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ508_001952"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2490901.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1136 a          927 c          734 g         1249 t
ORIGIN      
        1 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
       61 acaaaaaagt ccaatagtag gtcagatggc tatcactctc tagaacccga taagaggttt
      121 catccgcatg aagaagaggt tgttctaaca acttttctcg taaaagctta taaagggact
      181 ctaaatagta ttgacttgcc ttgatatgcc aattagagat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaaactg agccaaagct atgcaccaaa gagaccagca
      361 ccaacaacaa gataattgta catcttaagc tcctttggag ttcattatct gcctctttta
      421 ttagactaga aaatcattct actacttatc tcacttcttg tttgtaaaat tcttgcaatg
      481 catcttgcca agttggaata acaaatccag tagctttggc tttggccagg ctcatcgttg
      541 agtttagcgg acgttgagct ttggctggaa attgactgga atctactggc ttgacttcga
      601 catctgtatc tttcaaaatt tcaactgcaa aatcatacca tgttgtgtct tctgtcgcat
      661 catttgacaa atgataataa ccaaattcct tacgattttc agctaggtag atcatgaatt
      721 cagccaaggt acgagtccaa gtcggacgac cgtactggtc atttacaact gttaaagtct
      781 tatgagtttt cgcaagattt tgcatggtaa aaacgaagtt tttgccataa tttccaaata
      841 cccaggcagt acggataata tagaaattag acacatgctt ctcaactaac tcttccccca
      901 tacgcttagt gcgtccatat tctgtctgtg gatctggtcg gtcatcaact tcccactctt
      961 gtccaactgg tttcttaccg tcaaagacct agtccgtaga aatataaact agagttgcac
     1021 catgcttttc agatgctttt gcgacatttt ttgtccccat cacattgatg gcgaagtcca
     1081 actcttttcc ttcatcctct gctgcatcaa cagcggtgta ggctgcacaa tggtagacta
     1141 aagtcggttt cacctcttca aaaactttct caaccatttc ttcattggta atgtccatct
     1201 cagccacatc tactgccacg tattcttcat tacgttcatc caataaatag cgaagttccg
     1261 ttcctaattg gccatttgcc cctgtaatta aaatcatctc tagacctttc taatatgtaa
     1321 ctcttcccaa tatagcaata aaaagcagcg atttcctgct ttttatactg taataatctc
     1381 ctgagtctta gcataattgg cttcaacagc ttctttttct gctttccacc attcttggtt
     1441 atctgtatac cacttgattg ttgccttgag cccagcttca aagttggtaa attcaggttt
     1501 ccaccccaac tcatcacgga gcttgctggc atcaatcgca tagcgaaggt catgtcctgc
     1561 acggtcagtc acatgatcat aggcatccgt agcttgtccc atttccttaa ggataagttc
     1621 caaaacttcc ttattgttct tctcaccatc agccccaatc aagtaggttt caccgatttg
     1681 cccttttgtc aagattgtcc aaactcctga agaatggtca ttggtatgaa tccagtcacg
     1741 aacgttctta ccttcaccgt aaagttttgg cttaattcca cttaggatgt tagtaatctg
     1801 acgtgggatg aatttttcga tatgttgata aggaccgtag ttatttgaac agttggaaat
     1861 cgttgccttg actccaaaag aacgcaccca ggctttgaca atcaagtctg atgcggcctt
     1921 ggttgatgag tacggagagc ttgggttgta ttttgtttca gcagtaaatt tctcacctgg
     1981 tccttcacca tgacctggca aatcttctcg taaagggaga tccccataaa cttcatctgt
     2041 cgatacatgg tggaagcgaa tatcatactt acgagcagct tctaaaagag tatagattcc
     2101 aatgaagtta gtatgaataa atggcgatgg atcattgagc gaattatcat tgtggctttc
     2161 cgctgcataa tgaacgatag catctgcttg agcaaccaac ttgtctacca actccgcatc
     2221 agcaatgtca ccaacaacta actcaacacg attgcctaaa atttcctcaa tattcgcgcg
     2281 gtttccagca taagtcaact tatctaggac tgtcacgtga acatctggaa agttctcgta
     2341 aacataatgg acaaagttag aaccgataaa gccagctcca cctgtcacga taatattttt
     2401 gtattcagtc atattctttc cttattccaa atcttctttt ttcaaaggct tcacgtcctt
     2461 aagtagggga tgatgtttgt ctgcttcaga tacgtctgct tcttccagat tttcccattc
     2521 aataccaagg cttggatcag cgtagttcac aaaggcatac ttgggtttga gttcaagagc
     2581 ccagtaatca ttgaccagat agctatatga cactgtatct gataaaactt ggaagccatt
     2641 agctacgcct cgaggaacaa agattccctt gcttgcatca attactgtct gataggtatt
     2701 cccaaaggtt tcaccctcgc gtagatcaac ccaagaaccc agaactttcc ctccatctgc
     2761 tacagagatg tacttatccc aaggctctgc gtggaggcct cgaaggacat ttttacgtga
     2821 gaaggataca ttgttttgca attttccttc tgcaaagaaa gactctggaa atccaagtgg
     2881 aagcactttt tccttttgga aattttcttt aaaccagcca cgattatctc catgaacggg
     2941 gatatcaaac tccaacatgc ctggaatagc ttcaaccttg cgtgccgcaa gcgtcttacc
     3001 gaaaaaatta tctgtcatct atgcttctcc aatcaaacgg agcagatact gtccgtattc
     3061 atttttctta agtggttggg ctaaggccaa tacatcttct cgactgatat agcccatacg
     3121 gtaagcaatt tcttctaagt ttgctacctg aacattttgc atccgttgca ctgtttcgat
     3181 gtactgtgaa gcctctagta aactttcatg agttccagta ttcagccaag caaagccacg
     3241 tcccataagc tcaacagaca aatcaccacg atctaggtaa gccttgttta catctgtaat
     3301 ttccagttca ccacgagggc ttggtttaat actcttggca atttcgacaa catcgttatc
     3361 atagaaatac agtcctgtca ctgcatagtt ggagcgagga tactccggtt tttcttctat
     3421 ggaaatagca ttcatgtctg tatcaaactc aaccacacca aaacgctctg gatccttcac
     3481 ttggtagcca aaaacagtcg cacctttctc tttcttggct gctttttgaa gcattgtgct
     3541 cagaccagga ccatggtaga tattgtcgcc taaaatcaag gcaacgctat cgtcagaaat
     3601 aaactcttcc ccaattgcca aaccatctgt acttggttgc tctgcataag aaagtttgat
     3661 cccaaactca gatccgtctt gaagaagctc ttgaaatcga tgtaaatcct gtggagtgga
     3721 aataatcaaa atatccctaa tcccagccaa catcaatgtt gaaagtgggt agtaaatcat
     3781 cggtttatca taaaccggca taagttgttt tgacgcagcg cgagtcaaag gatataaacg
     3841 tgtccccgaa ccacctgcta gaataatacc tttcatagta gggtaccttt ctttgttatt
     3901 ttagagtcaa ttactattaa ttgtgtactg ctctaaaaag aaaacaatca tcccatgttt
     3961 tatatggctc tataatattt gtagtgggta aatccactat agatattatg gagcctattt
     4021 tattgtagaa aaaaagtccc ataaga
//
LOCUS       DAVBCR010000109         3949 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCR010000109 DAVBCR010000000
VERSION     DAVBCR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.109"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(418..1554)
                     /gene="dnaJ"
                     /locus_tag="VQ508_001953"
     CDS             complement(418..1554)
                     /gene="dnaJ"
                     /locus_tag="VQ508_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2490902.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1759..2112)
                     /locus_tag="VQ508_001954"
     CDS             complement(1759..2112)
                     /locus_tag="VQ508_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490903.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(2125..3948)
                     /gene="dnaK"
                     /locus_tag="VQ508_001955"
     CDS             complement(2125..3948)
                     /gene="dnaK"
                     /locus_tag="VQ508_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2490904.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
BASE COUNT         1036 a          891 c          789 g         1233 t
ORIGIN      
        1 atgacaagcc gacgctgacg tggtttgaag aaatttttga agagtatcat tcaccatttc
       61 acaactaaat cgaagacttt ctgccgtaat gaaaacacct gaaacagctt ggtttcagct
      121 gtccggaaac tttaagactt aggttcaaag tttaggtatg gaacttcgaa ggaggtcgct
      181 accgtccgtc attacttagg aaagtcttaa aaaatctata aagtaaaaag agcccgacag
      241 ctctttttgt ttatactcaa tgaaaatcaa aaagcaaact aggaaactag ccacgggctg
      301 ctcaaaacac tgttttgagg ttgtagatac gactcacata tatacggcaa gccgacgctg
      361 acgtggtttg aagagatttt cgacgctgac gtggtttgaa gagattttca aagagtatta
      421 ttctccatca aaggcatctt taatatggtc aaagaagcct tttttctttg gatttacttt
      481 caagtcacca gcagccgcga attctttcaa ggctactttt tggcggtcgt tcaagcctgt
      541 cggtgttacg acattaacag taacgtattg gtcaccaact gcaccgccac gaaggctcgg
      601 tgccccctta ctacgtaggc ggaatttctt accagtctga gttccctctg gaataaccaa
      661 ttcaacatca ccgtgaacag ttggaatatc tactgtatca ccaagagccg cttggacaaa
      721 gttgaggttg agattgtaga agatagtcgt tccttcacgt tcaaacttgt cgctagcttc
      781 tacagaaact actacataca agtccccata aggtccaccg ttaaagcctg cttcgccttg
      841 accagcgagg cgaatttgtt gacctgtttc cacaccagca gggattttca catgtacgct
      901 atgagcttgt ttctcatgac ctgttccatg acaggttgta catggatatt tgatttcttt
      961 tcctcgaccg tgacagacat cacaggttac ttggcgacgc atcataccaa gaggagtctg
     1021 cgtatcgacg ttaatgacac cagcgccatg acagcgtcca caagtgactg gacttgtccc
     1081 tggcttagca ccagagccat tacatgtacg acagccagct tcacgatgat acttaacttc
     1141 cttctcagtt ccgaagatag cttcttcaaa ggtcaaattg acacgatact ggagatcatc
     1201 tccttggcga ggagcgtttg gattgcgcga agaaccgcct ccgccgaaga aacttgaaaa
     1261 aatatcctca aaaccaccga agccacctgc cccattgaaa ccaccgaaac caccagctcc
     1321 accaaaacca ccattggcgc ctgcagcacc atactggtca taggcagcac gtttttggtc
     1381 gtcactcaaa gtctcatagg cttcttgaac ttccttgtac ttgtcctcag caccaggctc
     1441 cttgttgata tctgggtgat attttttgga aagcttacga taagcctttt tgatctcgtc
     1501 tgccgaagcg ttttttgaca cccccagacg atcataaaat tcagtattgt tcatacaaga
     1561 taccaagacc gtaaaattcg actgaaaaat aggaaatctg acgctggagc gacgctccta
     1621 gacagatttc tcttttttcc gagaatttag gtcgggttca attcctttct tttatattga
     1681 tgagataata ctccagccac tcggccctat ccctttttac cgaaaaacag aggctgagtt
     1741 gcaacctctg tggttagatt attcatttga aataaaaatg gttgtattat ctctagtagt
     1801 aatcttcaaa gaccttttag ctaaatcaaa ctcaaattta ttatagtgtg ggtcttgtgt
     1861 tttatatagg tgtaactcct gacatatatg atccgaaaag taaatcataa catctttaaa
     1921 cacagaagtg tggtcatatt tactcttacc aacagctact tctgtaaatt ctttataaat
     1981 gacatttcca tttctttcca cagtcaccag catttctgtt ggattataat ccaaattaaa
     2041 taattcttca aaaaacttta tcttttcatg ataatctgca atgtcagaaa gattattttt
     2101 attaacactc ataacactag ccccttattt ttcagtaaac tctccgtcta cgacgtcatc
     2161 gcctgcgttt cctgttgctt gtgcgccttc tgctccttct tgagcttgtt gcgctgctgc
     2221 agcttgttcg tagagtttaa cagcaagtcc ttgagctttt tcgttcaatg cttcaagttt
     2281 tgctttcatg tcgtccaagt tgttgtcttc ttgagctttc ttaaggtcat caagggcagc
     2341 ttgggcagcg tcacgttctg cgtcgaagcc tttaccttca gtttccttga ttgtcttttc
     2401 agtcgcaaag attgcttggt ctacttcatt acgaaggtct acttcttctt tacgtttctt
     2461 atcggcttca gcgtttgctt ctgcatcttt catcatgcgg tcgatttctt cgtcagtcaa
     2521 acctgagttc gattggatga caatagtttg ttctttttga gttccaaggt ctttggcctt
     2581 aacagacacg ataccgttct tgtcgatgtc aaatgttact tcgatttgag gaattccacg
     2641 aggtgcagct gggatatcag tcaattggaa gcgtccaaga gtcttgttat ctgctgccat
     2701 tgggcgttca ccttgaagaa cgtggatatc aacggctggt tggttgtctg ctgctgttga
     2761 gaagacttgt gatttagatg ttgggattgt agtgttgcga tcgataagtt ttgtaaatac
     2821 tccacccatt gtttcgatac caagtgacaa tggcgttaca tcaagaagga caacgtcctt
     2881 gacatcacca gtaatcacac caccttggat agccgcaccc atagcaacta cttcatcagg
     2941 gtttactgat ttgtttggtt ctttaccagt ttcagcttta acagcttcaa caacggcagg
     3001 gatacgagtt gaaccaccaa caaggataac ttcgtcgatt tctgacaagc tcaaacctgc
     3061 atctgaaagg gcttgacgaa ctggaacttt tgtacgttca acaaggtcac gagtcaaatc
     3121 gtcaaatttc gcacgagtca aagtcatttc caagtgaaga ggtccagcct cacctgcagt
     3181 gataaatggc aagctgattt gtgttgaagt tacaccagaa aggtctttct tcgctttttc
     3241 agccgcatct ttcaaacgtt gcattgccat cttgtcagta gacaagtcga taccgttttc
     3301 tttcttgaat tctgctacca agtggtcaat gattttttgg tcaaagtcgt caccaccaag
     3361 tttgttgtcc cctgcagttg acaatacgtc gaagacaccg tcacccaatt caaggataga
     3421 gacgtcgaat gtaccaccac caaggtcaaa taccaagatt ttttcttctt tgtcagtctt
     3481 gtccaaacca taagcaagag ctgctgcagt tggttcgtta acaatacgtt ctacttcaag
     3541 accagcaatt ttaccagcgt cttttgttgc ttgacgttga gcgtcgttga agtaagccgg
     3601 aactgtgata acagctttgg ttactttctc accaaggtag tcttcagcgt agcctttcaa
     3661 gtattgaagg atcatagctg agatttcttg tggagtgtat tcttttccat ttgcagaaac
     3721 tttttcagaa gttcccatct tagatttgat agagataact gtatctgggt ttgtaactgc
     3781 ttgacgtttt gcagcatcac caacgatgat ttctccgttt ttgaatgaga ctacagatgg
     3841 agttgtgcgg tttccttctg ggtttgcgat gattttgctt tcagttcctt caagaactgc
     3901 aactgctgag tttgttgtac ctaagtcaat accgataatt ttagacatg
//
LOCUS       DAVBCR010000110         3939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCR010000110 DAVBCR010000000
VERSION     DAVBCR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.110"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ508_001956"
     CDS             98..1357
                     /locus_tag="VQ508_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2490905.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ508_001957"
     CDS             1607..2107
                     /locus_tag="VQ508_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2490906.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ508_001958"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ508_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2490907.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ508_001959"
     CDS             2738..2908
                     /locus_tag="VQ508_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490908.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ508_001960"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ508_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ508_001961"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ508_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQ508_001962"
     CDS             3503..3883
                     /locus_tag="VQ508_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2490909.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT         1259 a          701 c          842 g         1137 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaccc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctga
//
LOCUS       DAVBCR010000111         3449 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCR010000111 DAVBCR010000000
VERSION     DAVBCR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.111"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            30..547
                     /locus_tag="VQ508_001963"
                     /pseudo
     CDS             30..547
                     /locus_tag="VQ508_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(513..1988)
                     /locus_tag="VQ508_001964"
     CDS             complement(513..1988)
                     /locus_tag="VQ508_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2490910.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2695..3449
                     /locus_tag="VQ508_001965"
                     /pseudo
     CDS             2695..3449
                     /locus_tag="VQ508_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1094 a          737 c          633 g          985 t
ORIGIN      
        1 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
       61 tttacaccta caatcctttg ctcatcaaga aatttgccaa atcattatca cttcgcaaga
      121 ctaaaacgga taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
      181 gtgaacaatt tcaacacaat aatcggcaag tagaactaaa aatactgact agacatattc
      241 atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgc cttgatatca
      301 tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac caactcttga
      361 cacgctaccc taatcctcag aaaaggattg aggcaggatt tgataagctg atagaaatta
      421 agcgattgac cgcttctaat tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc
      481 atcaaattga aataagaaat agtttagata aactattgac ttttcttata gtttgtcttt
      541 ctcttaataa cgttgatatg attgacctct ggatcttccg tcccttggag ggagcaacca
      601 cgttccttgg cgaattggat atagtcgaca atttctcgtg taatgcgttc accaggagcc
      661 aagataggaa tacctggagg gtaacacata acaaattctc cacagacctg tccaacagaa
      721 tcatccaaag ttaaactttt tctttctgaa tagaaggctt cttgcggaga cagcactaac
      781 tcgggctgaa tatattctcc tgctatcaaa tcttttccat ctcttgaata gagtctctta
      841 atatcagcca gagcaccaac caagcgctcg atgtcttgga tgcggtcgcc gatggaaata
      901 taggccaaga tattgccgat atcaccaaac tcgatctgaa tgtcgtattc gtctcgcaag
      961 aggtcataaa cctcgatacc tgttaagcca ataccctgag tgtaaactga caacttagtt
     1021 acgtcaaaat cgcaaaccga aacaccgtct attaactctt ttgagtaggc atagtagcca
     1081 ccgatagcat tgatttcatg acgagcgtac tcggatagct caatgacttc ctcaaacgac
     1141 tctttaccac gaagggccaa gttgcgacgt gaaatatcca aactagccat caataagtaa
     1201 gaggcagatg tagactgggt caggtttata atctgacgaa cgtactcggg attcatctgc
     1261 tccccgatta aaagaagcga actttgtgtc aaactcccac cagacttatg catggaaact
     1321 gctgccatat cagctcctgc atccatagca gaaattggaa gtttaccagt gaaatgcaaa
     1381 tgcgctccat gggcttcatc tactaaaacc atcatgccag cttcatgagc catttctgtc
     1441 aaccccttta ggtctgaaca gatgccgtag taagtaggat tgttgattag gatagcctta
     1501 gcatctggat ggtcctttat ggcctgtgct actcggtcat tttcaagacc taaagcgata
     1561 ccaatcttag gatctacact catctcgata tagatgggaa tggcaccaca tagaaccaac
     1621 gcattgatag cagatttatg gacatttcgt ggcagaataa tcttatctcc tgccttgcag
     1681 gttgccagaa tcatagtctg caccgatgaa gttgttccac caatcattag aaaggcatgg
     1741 ctagctccaa aagcatctgc agccagctcc tctgcatcac gaataatcga aataggatgg
     1801 cctaaattat ccaagggttt catcgaattg acatcaatgc ctacacattt ttctcctaag
     1861 agttcgacaa gttctggatt tccccgtcca cgcttgtgac ctggaacatc aaagggaaca
     1921 atccttttct tgcgtaactt caccaaggcc tcataaattg gggcttggtt ttgatctaac
     1981 tctttcaaaa catactcctt tcaatatttt taggacctta tgaaaactaa ggaactaaag
     2041 gttgactcat gaaaaaagag caggttttca cctgctctgc aatcttctga cgtgtttatt
     2101 ttggtttctg ttgagtatgt ctgttcccga tgtttcctaa ctctctagta gcaaatatta
     2161 cgtactttat tgatcgtttc acaagatgac gtgtgctttc accacactaa aaatttatga
     2221 attaggacaa gtgtcctaac atcaacttat aaaagtaggc ttttaaccct atcaataatg
     2281 tggcttttca accacgcttc ggcattcaac cctgcctgtc cgtaggcatt ttttactcgg
     2341 gtttatattt gctagaaaac atcatctcat tttctaagct ttattactat atcatgtttt
     2401 taagaacttg taaagtattt tttgataaaa aacaaaaata taatagattg aaactagaat
     2461 agtacacctc tgcttctaaa acattgttag aaatagattt tactgtcctg atcgatttgt
     2521 tctgttcttg tttcatttta ctatatgttc gtaaatgaaa gttctataat gaagttagcc
     2581 acccagccct cctaaaaatt agatggttca actagctgat agctcttgta ggcataagaa
     2641 aacacctctg tgttatactt gttgttcaac cacaaacaca agaaaggcac agaaatgcaa
     2701 gacaattata ctacaaaagg taaacatttg acaatcgata gccgtcgctt aatcgaaaga
     2761 tggaaaaaag aaggaaaatc aaatagagaa attgcctctc tacttggaaa agctcctcaa
     2821 actatccaca ctaaaatcaa gcgtaggaca gtccgaaaat gtcttggaaa agggcgcttc
     2881 aaagaggttt attctgccga ctatgctcaa cagtcttatg aaaataatcg caagcactcg
     2941 gtcaagagat caagcgtgac caaggaacta aaggaaaaga ttctccacta tcataaccaa
     3001 aaatttttgc ctgaaatgat ggttatggct aaaggggtta acgtgggaat ttcaaccatt
     3061 tactattgga ttcatcatgg aaaattgggg taaagcaagc aggatctgct ttaccctaga
     3121 aaaggaaaag tgcttaagaa acaggccagc accaacttta aacctgctgg tcaatccatc
     3181 gaacagcggc ttgaagctat caattttcgc ttggagaatg ggcattatga gattgatacg
     3241 gttctactta cgagagcgaa aaactactgc ttgcttgtct tgacggatcg aaaaagtaga
     3301 catcagatca tccgattgat tccaaataaa agtgctgagg tggtcaatca ggctctaaaa
     3361 ctcatcttaa aacaacacaa gattctttcc atcacggcag ataatggaac ggaattcaat
     3421 cgcttgtcta atgtattttc tgaggagca
//
LOCUS       DAVBCR010000112         3262 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCR010000112 DAVBCR010000000
VERSION     DAVBCR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.112"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ508_001966"
     CDS             complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ508_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2490911.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1022..2074)
                     /locus_tag="VQ508_001967"
     CDS             complement(1022..2074)
                     /locus_tag="VQ508_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2490912.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2071..2769)
                     /locus_tag="VQ508_001968"
     CDS             complement(2071..2769)
                     /locus_tag="VQ508_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490913.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2936..>3262)
                     /locus_tag="VQ508_001969"
     CDS             complement(2936..>3262)
                     /locus_tag="VQ508_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2490914.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          912 a          735 c          626 g          989 t
ORIGIN      
        1 ttttttacga aaatctattg aatatgccat aaaaggatta taccacattg tgtactattt
       61 ttggttcatt ttactatatc actttggact atatcggtct agttatgtct aaacgattat
      121 agctggatac taagagaaat caaaaaagca ctaagggaga acgtcccaaa gtgcttaata
      181 tcaaggcctc aaagcctgtc ttattcaata aaatattaca acatcttgtt gtagaattca
      241 acgacaagtg cttcgttgat ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat
      301 gaaccttcca atttttcagc gtcgaatgat acgaatgctg gacgtccaag agtagcttct
      361 actgcttcaa ggattgctgg aactttcaat gatttttcac gaactgagat cacttgacct
      421 ggagttacgc ggtatgatgg gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg
      481 tttacgaatt gacgagcttg acgacgagta gtcgcgagac caagacggta aacaacgtta
      541 tccaaacgac gttccaaaag aagcataaag ttgaaaccta ggattccgcc tttgattttt
      601 gtagcttgta cgaacaagtt acggaattgt ttttcaccta caccgtaagt gaaacgaagt
      661 ttttgttttt cagccaattg caaaccgtat tctgacaatt tagaacggtt gtttggtccg
      721 tgttgtcctg gtacgtagtt acgacgtgcc aattctttac ctgtacctgt aagtgaaagg
      781 ccaaggcgac gagcttgttt ccaagatggt cctgtataac gtgacatatg aatgtcctcc
      841 tgatataaat aatatttcgg tggaaatagt cacttagaaa gccctgattc gtgcagatgc
      901 ccttcgccta aacagccaag gttacttgtc ataagacacc tgttgacgag cttcatgctt
      961 tcctgctgat atttcacaca aaatccattt taccatgaat agctggattt gtaaaggggt
     1021 tttaggcttt attttcacta ccagagagat tgagaacgag ggtaaaggta cttcctagac
     1081 cgtactggct gctgactgtg atttccccac ccaattgatg ggccaattca cgcgcaatcg
     1141 caagtcctaa tccatgacca cctgtcttca tgttacgcga agtttcgaca cgataaaggc
     1201 gtttgaaaat attttccaaa tcctctgggg caatgccctg cccttcatcg gtcacactga
     1261 ttgaaagctg gcccttctcc agcttagtca ccacttccag cttggttcct ggagcagaat
     1321 atttaaaagc gttatcgacc agattcacca agatacgaga aagcttagca taatctccct
     1381 caatccgggc agactctggg attacctgca agtggacatc tcttctctcc tgctcaatca
     1441 aaaactgaaa ttcactcatg cactcaatta agagcttgtc cagaaaaata ctgtctttac
     1501 tggtagtttc cacctgattt ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca
     1561 gtttattgag cctctccgtc tggcgtccaa tggttgctag ataatgagct tgctccgact
     1621 ccttgataat cccatccaaa atcccttcta ccgtcgcttg gatcgaagtg ataggggtct
     1681 taatatcatg cgacaactgg gcaatcatca agcccttttc tcgttcgctt tcttccaagg
     1741 aatcaaagct tacctgcaaa tcatgggaca tctcattaaa agtttgccct aattgctgaa
     1801 attctacagg accttgaacc tccaaatttg aaggaaaatc cttggccgct acccgcttgg
     1861 catgctcctt gagtttgccc aacgacgtaa agactggcaa taggagaaag agactaatcc
     1921 cagcaccgac aaggctggca acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg
     1981 caatcagcat tttttgaaca gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa
     2041 tatatccaac caaaatataa ctttttagtt tcatgtttgt cctctcggtt tctctatctt
     2101 atatcccaac ccccaaactg tcttaatagt gggcgtttgg tcactactat attttgccag
     2161 ctcctgtcga agagcatgga tatgcacatt caaggtattg gtgtcatcca cgtagtcttc
     2221 tttccagatc ttttcataga ggtctgtctt ggagaaaact cgctctggat tgctagctaa
     2281 aatccacagc aattcaaatg atttaacagt taaatccagc atttcttctc ctatttgaac
     2341 ttcatgacta ctatgattca tttttagatt gccaagggaa atcagctctg tttcgccccc
     2401 acgatgaagg cggcgcaaaa tattgtggac acgcaaaacc agctcacgtg ggctaaaagg
     2461 cttagcaata aaatcatctg ctcccaagct caggccgtaa atcttgtcct gttcactggt
     2521 cttagcagta ataaatagga aaggctgctc tggtgataag tattgaacct cactgattaa
     2581 atcataacca tccatccgag gcatcataac atctgtgata atcaaatcaa tcggtttttt
     2641 cttgaacagc tctagcgctt ccagtccatc atgggctacc aagacctgat aacctgcctg
     2701 aattaagtat ctctgatgaa tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt
     2761 ctttcccatc tgtctctcct ttgataaaaa cagtgctata ctgctttaag tataacacta
     2821 tttttattgt tttacgatga ttttaatctg ctattgaaag aaagtcctga ttgctgattg
     2881 agttgttaaa ctaatcttag tcagcccact atccatcatt tatttcctta gctgattagt
     2941 tttcttcttt tttgtttttc aaacctagac caccgagtaa acctgcaagc gcaagcccaa
     3001 ggaaaccaat acttgccatt gatgtttgag tctcaccagt atttggtagc atagctttat
     3061 cctctgacat catcgtatca gacatcttgt tagcagaagc agccatgttt tcacctgcca
     3121 tcgtgttggt agaacttgtc atggtgtcag caggcatgct atctgtagta cctgtagcat
     3181 gattgtgatt catcggagtc acgccagaac cagagttaga aggtgataat gaaccatttg
     3241 ctgtgtctga tgtttcttta ac
//
LOCUS       DAVBCR010000113         3243 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCR010000113 DAVBCR010000000
VERSION     DAVBCR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.113"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            169..396
                     /locus_tag="VQ508_001970"
     CDS             169..396
                     /locus_tag="VQ508_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490915.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYNKINLI
                     NSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            553..1611
                     /locus_tag="VQ508_001971"
     CDS             553..1611
                     /locus_tag="VQ508_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490916.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1926..2900
                     /locus_tag="VQ508_001972"
     CDS             1926..2900
                     /locus_tag="VQ508_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490917.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
BASE COUNT         1079 a          422 c          567 g         1175 t
ORIGIN      
        1 ggtgttggtt gtaactggtg gaacaaacgt gataatattg ctgcagctgc agtttctgga
       61 gggatgtcag tataccagac atatgccatt aaaaagttaa ttgctaatcg tggtatgatg
      121 agccgagtcg ttagaagtgc tataacaaga tatattggtg gagttagtat ggcaactatt
      181 actaatgctc ttaatattgc ggctactgtt gctgaagttt tctcattagg aggagccatt
      241 gcatatggtt tagatatcgt agacggtaaa tttgatggat ataataaaat aaatctaata
      301 aattctatta ttatagctct tggtatgata ttggcattaa ttttggtatt atcaagagtg
      361 tatagaaaat atttttatag agacgatgat gactaaagtt tttatcaata atttgggctc
      421 cttgtcaact gtagtggatt gaagaaaagc taacgtctgg ggaagggttt aaagctttgc
      481 tttatcaaat attgacttac agagttgctc tattgtattg atgtattctt agtaaataat
      541 atggtgtttt gaatgaaaga tgatcagaaa tatttattag cgggacttta ttcgcttcta
      601 gttgcaatat tttattttcc attgattgag tccaagggga tttttgtaag tatattgatg
      661 gcggttttac ttttatattt aatatacttc attgcaacag tcattcatat tgtgataatt
      721 aagtttataa gaaaaaaatc ttttaagtat ttggttctat atccatttac ttatgatggg
      781 agctggagat ttcaacctat aaacttactc tactttccag aaatggtgag agatgtcatt
      841 ccgattaatt tagttcaaga atattgccaa ggacaacctt acggactgtt aaaaaagatg
      901 ctgaaaagaa ttcgattgtc acgagagata gctttactcc tggctaccat tatcgtctat
      961 ttttttacgc atagaattct acctctttca gtatttacgt tcatattttc ttacatattg
     1021 ctatttgcac aatcttattt gggtggtaat accgtttgga ttggaaatag aagattaata
     1081 atagatgatg aatttgaaaa aatcttgctt tcaaaaagtt atataaaaga aatatcatct
     1141 gcacgatact cggaatactt gacatgtgaa tacaaaaacc ttacaccaat catattgtta
     1201 gctatttttg aaaatttgct tgactcctac ttgcttcaga atcagagtga ggttgactta
     1261 gatatttttt ataaagtttt acctttgctt tataaagaaa aatatactat gggattcaat
     1321 tactttgtaa gtttaaatta tttactatat aaggttggat ttttgggaat catttacgat
     1381 aatgaagcat taggagattt gtcaaaacaa tatttaaaca gaaatatttc ggaattacaa
     1441 gatggttctt tcgatggcgg tatacaagat gctgttgcct ctaaacaaat agtagttata
     1501 aatgaattta tagcttgttt aaattccaga tgcatgccat cccaatatga tagatttttt
     1561 tataaagata gaccttatat tttttccaga aagagtccta ttaaggggtg aaaatttgtt
     1621 acatttggat taacaagtgt cctagggatt agaaataact ctagtatagc tagatatttt
     1681 tgagtaacta gaaatatcat taggctaccc atatgattgc cagaaaagat ggctgatgaa
     1741 tcttatttat actgttgcag taggcattgt ctaggataaa taagctatct tgatggggga
     1801 aattggaata attttagtag aaacattaaa ggattatgga caagtagcgc aggattaggt
     1861 gatgcaatgt gctaagttgg cttggtgtta gcagtagtat tatttaacaa tggaaagtag
     1921 actaaatgaa aaataagcgt tatttttttg acacaatttt aatgatttta ctgcttataa
     1981 gcacgatatt ttgtgtcagc ccagtattta taaaattgga catcttagga accccctctc
     2041 atgccatctt aacctttgta ttggcgatac cgttattcta tattttgtct caatgcttac
     2101 atactttgct attactagtt tcctcaatct tttgtaaatt aagaccaatt tatttttatt
     2161 ttatatttgt gatcattata ggagctagga aatactatcg aattctcttt caccaactaa
     2221 tgggatttag tccaggaata gcagtgtttt ataaggagag tcaaacaact aagaatctgt
     2281 ttaaatttta ctacttcttg tactttacaa cactaatttc atactatttt ttctttactt
     2341 ttgtgtatga caaaccgttg cttttgcctc ttattccatt ttctattatc atagcattag
     2401 tacaaaaact ttatagaatt gagaatcaac aattatttct gttaaaatca aaggtactaa
     2461 caatactgga atctaagaag aattgtgagt ttaatcttca ggattatcat gaaatttgga
     2521 aattgcaatc aaaatctgag ttgccatgcg tggctttatc ttatatcagt ttgatcaagc
     2581 catatctttc agagagcgtg cgtgaacaaa ttgatttatt agaagttaag cgatttaaaa
     2641 aaataaatca tccaatatca ctttatggta tgttggatgt tataaaatta aatctatatt
     2701 tacggcatta taatgaaaaa aataaatatg aatcaatgtt aaagaaaatt ttagaggtaa
     2761 gaccagactt tgttttaatc gagcagaata ttgatgatag tttaaatagc tcgcaaccat
     2821 tatctttatc tttagccatt tcagaaatcc aacttttgct tgaagtgtat atggggatca
     2881 aacatgtttc aaccaagtga taaaaccaga ataatatttg ccattatagg ggcaagttgc
     2941 atattagtca gtatgtatct tattccactt attaaaactg agaataaatt ttgggaaata
     3001 cttttattta tattaggtgt attgctaatt ggaattgcgt tactaagtca aaaaaaataa
     3061 attttgaaat agtattagtg agttcgcaat catttattgc tagtggttta gaactgttaa
     3121 gtttcaaaca ataattgatt ttaaacctta ggtaacctat aaaaacaaat aaggtagaga
     3181 aaggatatct tatctaggta gctaagtgat aaaaacacag tcgtctatca cttgttagga
     3241 aac
//
LOCUS       DAVBCR010000114         3178 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCR010000114 DAVBCR010000000
VERSION     DAVBCR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.114"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ508_001973"
     CDS             complement(381..914)
                     /locus_tag="VQ508_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2490918.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ508_001974"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ508_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2490919.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINVNQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ508_001975"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ508_001975"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2490920.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1023 a          560 c          578 g         1017 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattgacatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaagg
//
LOCUS       DAVBCR010000115         3060 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCR010000115 DAVBCR010000000
VERSION     DAVBCR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.115"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            143..901
                     /locus_tag="VQ508_001976"
                     /pseudo
     CDS             143..901
                     /locus_tag="VQ508_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            941..1705
                     /locus_tag="VQ508_001977"
     CDS             941..1705
                     /locus_tag="VQ508_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2490921.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1718..2395
                     /locus_tag="VQ508_001978"
     CDS             1718..2395
                     /locus_tag="VQ508_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2490922.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2405..>3060
                     /locus_tag="VQ508_001979"
     CDS             2405..>3060
                     /locus_tag="VQ508_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2490923.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     "
BASE COUNT          841 a          731 c          584 g          904 t
ORIGIN      
        1 attgttagaa atcgatttga ctgtcccgat cgatttgtcc tgttcttatt tcactatagt
       61 gaattttttc agatatggtt tgacaagaac attctttcat tgtagaatcg tgttataaaa
      121 tataacacaa aggagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta
      181 cggagactac cacgcactcc gcaacatcaa tctccgtttt gaaaaaggac aagttgttgt
      241 cctgcttgga ccttctggct ctgggaagtc cactcttatc cgtacgatca atggtttaga
      301 ggctgttgac aaaggaagtc tcctagtcaa tgggcaccaa gttgctggtg ccagccagaa
      361 agatttggta cctcttcgca aggaagtcgg catggttttt caacatttta acctttatcc
      421 acacaaaacg gtgttagaaa acgtgacact tgcgcccatt aaagttctag gaattgataa
      481 aaaagaagct gaaaaaaccg cccaaaaata tctggaattt gtaaatatgt gagacaagaa
      541 agattcctat cccgccatgc tatctggtgg acaaaaacag cggatcgcca tcgctcgtgg
      601 tcttgctatg catccggaac tcctcctctt tgatgaacca acatctgctc ttgatcctga
      661 gactatcgga gatgttctag cagttatgca gaaactggcg catgatggga tgaacatgat
      721 catcgttacc cacgaaatgg gctttgctcg agaggttgcg gaccgcatta tctttatggc
      781 cgacggagaa gttttagtag atacgacaga tgtcgataac ttttttgaca atccaagcga
      841 acctcgtgcc caacaattcc tcagcaaaat tatcaaccac gaaagtgaca aagtcaaata
      901 aggaggcgcc tatgaaaaaa aattcttttt atcagcatta ttgattagcc ttttcggcct
      961 tgctgctgcc aaaccagtcc aggctgatac tagtatcgca gacattcaaa aaagaggcga
     1021 actggttgtc ggtgtcaaac aagacgttcc caattttggt tacaaagatc ccaagaccgg
     1081 tacttattct ggtatcgaaa ccgacttggc caagatggta gctgatgaac tcaaggtcaa
     1141 gattcgctat gtgccggtta cagcacaaac ccgcggcccc cttctagaca atgaacaggt
     1201 cgatatggat atcgcgacct ttaccatcac ggacgaacgc aaaaaactct acaactttac
     1261 cagtccctac tacacagacg cttctggatt tttggtcaat aaatctgcca aaatcaaaaa
     1321 gattgaggac ctaaacggca aaaccatcgg agtcgcccaa ggttctatca cccaacgcct
     1381 aattactgaa ctgggtaaaa agaaaggtct gaagtttaaa ttcgtcgaac ttggttccta
     1441 cccagaattg attacttccc ttcacgctca tcgtatcgat gccttttccg ttgaccgctc
     1501 tattctgtct ggctacacta gtaaacgcac agaactacta gatgatagtt tcaagccatc
     1561 tgactacggt attgttacca agaaatcaaa tacagagctc aacgactatc ttgataactt
     1621 ggttactaaa tggagcaagg atggtagttt gcagaaactt tatgaccgtt acaagctcaa
     1681 accatctagc catactgcag attaaggagg acaccccatg atagatttat catcttggac
     1741 agcctatttt caggattttg gacaattttt caatggtttc ctctttactc ttgccctagc
     1801 gattggatcc tttatcctcg ccatggtctt aggaatcttc tttggtgcca tgtcaaccag
     1861 taaacgtcca attttacgca ttttagctca catctttgtg gaattttacc aaaacactcc
     1921 cctcttggtg cagtttgtta ttgtttttta tggcctacct cttatcagtg accatactat
     1981 catgattcca atttattgga cagctgttct ctgcatggga ctctatcacg gcgcttatat
     2041 cgctgaggtt attcgttcag ggattcagtc tattcctagt ggtcagatgg aggccgcctt
     2101 gtcgcaaggt tttacctata tcagtgccat gcgcttgatt atcttgcctc agaccttccg
     2161 catcattctc cctccattga ccaaccaaat tgttaacctc atcaagaaca cctctactgt
     2221 agctatcatc tctggagtag acttgatgtt tgtgactaag tcttggtcgg ctctcaacgg
     2281 aaactatatc ccagcctttt taggtgctgc tcttctctac tttgctctat gcttccctgt
     2341 tgcccagttt ggtcgcaaga tggagcaagc caacaaaaaa gcctattcac tttaggaggt
     2401 tactatggaa tctattttag aagttttaac cccagataac ctagtcttta tctttaaagg
     2461 atttggcttg accctctata tttctctgat tgccatcatc ctctctacta tcatcggtac
     2521 ggtgctagct gtcatgagaa atggcaaaaa tcctgtctta cgcattattt ccagtattta
     2581 tatcgagttt gtgcgcaacg ttcccaacct tctctggatt tttactatct ttttggtgtt
     2641 caaaatgaaa tccacaccag caggtattac agcctttact ctctttacat cagcagcctt
     2701 ggctgagatt attcgaggcg gtctcaatac cgtagacaag ggacagtacg aagcaggaat
     2761 gtcacaaggc ttcacctcag cccaaatcct ctactacatc attctcccac aagccatccg
     2821 caaaatgcta ccagccatca tttctcagtt tgttaccgtg attaaggata ccagtctcct
     2881 ctactctgtt atcgccctac aagaactctt tggagccagc caaattctca tgggccgtta
     2941 tttcgaacca gagcaggtct tcagtctttg catcctgatt gccctcatct acttcagctt
     3001 taacctagca atttctagcc tgtctcatat gctagccaaa cgttggcaac aagctgcaga
//
LOCUS       DAVBCR010000116         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCR010000116 DAVBCR010000000
VERSION     DAVBCR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.116"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(91..1140)
                     /locus_tag="VQ508_001980"
     CDS             complement(91..1140)
                     /locus_tag="VQ508_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2490924.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1137..1868)
                     /locus_tag="VQ508_001981"
     CDS             complement(1137..1868)
                     /locus_tag="VQ508_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2490925.1"
                     /translation="MLEIKNLTGGYVHVPILKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1936..2346
                     /locus_tag="VQ508_001982"
     CDS             1936..2346
                     /locus_tag="VQ508_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2490926.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2356..2643
                     /locus_tag="VQ508_001983"
     CDS             2356..2643
                     /locus_tag="VQ508_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490927.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT          967 a          648 c          549 g          727 t
ORIGIN      
        1 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
       61 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
      121 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
      181 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
      241 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
      301 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
      361 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
      421 ggaaagtaat agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
      481 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
      541 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
      601 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataacatagt cccagtccag
      661 tccagtttca gtaaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
      721 caataaaagc acatatagca gaaaaactgg caagccataa cccattgcta aaaataaagg
      781 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
      841 ttgacgcttg agatggagct tgattgcctc ttctccaact aagagaaaga gcttgtctgg
      901 agcctccata taggtggcaa ttcctcccca aagtaagagt aaaacagacg taattcctac
      961 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     1021 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     1081 cagataacaa agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     1141 agatcctcct ctttggtcag agccaagtaa atatcattca aactagcctc aggcatatca
     1201 aaggcttcac gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     1261 acaaaggcat cacacatctt ttccgccgaa tccagcacgt gggtactcat gagaatagac
     1321 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     1381 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     1441 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     1501 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     1561 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     1621 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     1681 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     1741 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     1801 gtaaaggaca catctttcaa aataggaaca tgaacatagc cacctgtcag gtttttaatt
     1861 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     1921 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     1981 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     2041 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatgaatgct
     2101 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     2161 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     2221 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     2281 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     2341 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     2401 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     2461 cgcgttcaag ctatcaaaga cgatgtggat attatccaaa atagcctgca aatcatcgac
     2521 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     2581 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     2641 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     2701 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     2761 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     2821 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     2881 aacaccgttt t
//
LOCUS       DAVBCR010000117         2839 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCR010000117 DAVBCR010000000
VERSION     DAVBCR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.117"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            86..2782
                     /locus_tag="VQ508_001984"
                     /pseudo
     CDS             86..2782
                     /locus_tag="VQ508_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          876 a          458 c          623 g          882 t
ORIGIN      
        1 cttgtgagat agctataaaa gtgtggagaa atagcttgaa atttagtata atatagctaa
       61 actatttgtt taaagtgaga aaaaaatggg aaattttagc tttcttttaa aaaatgacga
      121 atatgaatct ttttcaaaac cttgcattga agctgagaat atgattgcta catcaactgt
      181 ggctactgcc tttatggcgc gtcgtgcttt agagcaggct gtccattgga tatatagtca
      241 cgattcatat ttagaagctc cctatcgtgc tactctatct tctttagtat gggatgatga
      301 ttttagggat atcgtagatt ctgaactcca caagcagata gttctgttga ttcggtgggg
      361 aaaccatgct gctcatggtg gtgaaattaa ggaacgagaa gcgattttag ctttgcatca
      421 tttgtatcag tttgttaatt ttatcgatta ttgttacagc aatgagtttg tggagcgtta
      481 ttttgatgag aagtgcttac cactttcagc aaacatcaaa taccgagaaa ctccacaatc
      541 tatgataaag ttacaagaca gtttaccaga actgcctgat tttcatgaac agatggctgc
      601 tcagtccgta gaagttcaag agacttatac tgaaaaacgt gagactgcag cgcaacggca
      661 agatgtgcct ttccatattg atcaattatc tgaggcagag acaagaaagc tctttattga
      721 tatcgatctc cgtttagcag gatggatatt tgaagaaaac tgtcgtgttg agatagccgt
      781 tgatggtctc aagcacggtt caggaattgg ttactgtgac tatgtacttt atggtaaaaa
      841 tgggaaaatt ttagcgattg tggaggctaa aaaagcctct gtcaatccag aagtagggga
      901 agtacaggtc aaagaatatg ctgaagcttt ggagaaacat atcggctatc agccaatttg
      961 ctttattaca aatgggttga agcactatat acttgatggt ccgaaccgcc gccagattgc
     1021 aggcttttac tctcaagaag aattgcaatt agtgatggat agacgtcatc ttcaaaaacc
     1081 gcttgaggat atttctagta aaattaggga cgatatttcc gggcgtcact accaaaaaca
     1141 tgccattgca agcgtttgtg aagctttctc tgatcatcgt agacaggcac ttttggttat
     1201 ggcaactggg gcggggaaaa ctcgtacagc agtttctcta gttgatatct tatcacgtca
     1261 taactgggta aaaaacgttc tcttcttagc cgatagaact tccttggtta agcaagcata
     1321 tgattcgttt agaaaattac tcccagatct ttccgtttgt aacttcttag aagataaaga
     1381 aggagctcaa tcaagtcgaa tggtcttttc aacttatccg accatgattg gagcgattag
     1441 tggtcaagaa gaagtaaatc aacgcccttt tactgttggg cattttgacc ttatcataat
     1501 tgacgaatct caccgttcta tttatcagaa atacaagtcc atttttgatt attttgatgc
     1561 aaggattgta ggcttaacag ctactccgcg tcaagattta gataaaaaca cctatggatt
     1621 ctttaatttg gagaatgggg ttccaacata tgcatatgat ttggaagagg ctgttaaaga
     1681 cggatattta gtagcctatc attctatcga aaccaaactg aaactaccta cggatggtct
     1741 acattatgat gatttgtccg aagaagaaaa ggaacatttt gatagcaaat ttgaagacga
     1801 tagctgtgaa aaagatattg atgggagtgt gtttaattcc tttattttca ataaaagtac
     1861 agtagaaatt gttttaaatg aactcatgac aagaggaatt cagacagcct cgggtgatga
     1921 aattggtaaa actattattt ttgctaaaaa tcatgaccat gcggaatata tcagaggtat
     1981 ttttaacaac cgctatcctg aaaaagggag cgactatgct caggtgattg attatagtat
     2041 taagcattat cagaccttga ttgatgattt taaaattaag gagaagtatc ctcaaattgc
     2101 gatttctgtc gatatgttag atacaggtat tgatgtacca gaggttgtta atttagtctt
     2161 cttcaagaaa gtacgctcta aaactaagtt ttggcagatg attggtcgag gaacccgtct
     2221 atgtaaagat ttatttggac ctgagcagga taaggaaaac ttcttggtat ttgattatgg
     2281 ggacaatttt gattattttc gtgcagatcc aagagatgga gagggtcgtc acattgtttc
     2341 gctgactcag cgtttattta atatcaaagt ggacttgatt cgagaacttc agggactcca
     2401 ataccaagaa gatcagtttg cgagagcata ccgtcagcag cttgtctcgg aacttcaagg
     2461 tcgtatagag agcttaaatg agttggactt cagggttcgt atggttttag atacagttta
     2521 tagctatagg aaattggaaa gttggcagaa tctaactgct gttacaagtg aaaccattca
     2581 aaaaaatctc tctccgcttt tatttgatga agataaagaa gatgagatgg cgaggagatt
     2641 tgatttgtgg ttgcttcata ttcagttggg gcaactgaca gctaaatctt ccactgttca
     2701 tatttcccaa gtgatgaaga cggctagagc tctttctgct attggcaata tcccgcaggt
     2761 ttttgagcag gctgaaatta tggctctttg tcaactgtag tgggttgaaa aaaagctaag
     2821 cttgagaaag gacaaattt
//
LOCUS       DAVBCR010000118         2707 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCR010000118 DAVBCR010000000
VERSION     DAVBCR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.118"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            239..2563
                     /gene="pflB"
                     /locus_tag="VQ508_001985"
     CDS             239..2563
                     /gene="pflB"
                     /locus_tag="VQ508_001985"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2490928.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKSNFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          856 a          560 c          533 g          758 t
ORIGIN      
        1 ttcgatttcc attgagtgtt actgcttatt ttcttttatt ataccctttt ttctgaaaaa
       61 aagaaaaaag gactttattt tttcaaaaat ataatacagt ttgaaataaa atatagactg
      121 ttttagaaaa gaaagtgtaa aaatagggaa ttttcacttg ttgaaatcgg ttactatatg
      181 gtatacttgt cttatgaatg taacagatga ctgttactag aaaaaagagg acattaatat
      241 ggttgttaag acagttgttg aagcacaaga tatttttgac aaagcttggg aaggcttcaa
      301 aggcgtagat tggaaagaaa aagcaagtgt atcacgattt gtacaagcta actacacacc
      361 ttatgatgga gacgaaagct tccttgcagg accaacagag cgttcacttc acatcaagaa
      421 aattgtagaa gaaactaaag cacactacga agaaactcgt ttcccaatgg acactcgtcc
      481 aacatctatc gctgatatcc ctgctggatt tatcgacaaa gaaaatgaag ttatcttcgg
      541 tatccaaaac gatgaactct tcaaattgaa cttcatgcca aaaggtggta tccgtatggc
      601 tgaaactact ttgaaagaaa atggatacga accagaccca gctgttcacg aaatcttcac
      661 taaatatgta acaacagtta acgacggtat tttccgtgcc tacacttcaa atattcgtcg
      721 cgctcgtcac gcacacactg taactggtct tccagatgca tactcacgcg gacgtatcat
      781 cggtgtttac gcacgtcttg ctctttacgg tgcagactac ttgatgcaag aaaaagtaaa
      841 tgactggaat gcaatcaaag aaatcgatga agaaacaatc cgtcttcgtg aagaagtaaa
      901 ccttcaatac caagcattgc aacaagttgt tcgcctgggt gacctttacg gggttgatgt
      961 tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg gttaacattg ctttcatggc
     1021 tgtctgccgt gtgattaacg gtgctgctac atctctaggt cgtgtaccaa tcgtattgga
     1081 catctttgca gaacgtgacc ttgctcgtgg tacatttact gaatcagaaa tccaagaatt
     1141 cgttgatgat ttcgttatga aacttcgtac agttaaattt gctcgtacaa aagcttatga
     1201 ccaattgtac tcaggtgacc caacctttat cacaacttct atggctggta tgggtaacga
     1261 cggtcgtcac cgtgttacta agatggacta ccgtttcttg aacactcttg acaacatcgg
     1321 taactcacca gaaccaaact tgacagttct ttggactgac aaattgccat acaacttccg
     1381 tcgctactgt atgcacatga gccacaaaca ctcttctatc caatacgaag gtgtaacaac
     1441 aatggctaaa gacggatatg gtgaaatgag ctgtatctca tgctgtgtgt ctccacttga
     1501 tccagaaaat gaagaacaac gccacaacat ccagtacttc ggtgctcgtg taaacgttct
     1561 taaagccctt cttactggtt tgaatggtgg ttacgacgat gttcacaaag actacaaagt
     1621 atttgatatc gaaccaatcc gtgacgaagt tcttgaattt gaatcagtta aatcgaactt
     1681 tgaaaaatct cttgactggt tgactgacac ttacgtagat gccttgaaca tcatccacta
     1741 catgactgat aggtacaact acgaagctgt tcaaatggcc ttcttgccaa ctaaacaacg
     1801 tgccaacatg ggattcggta tctgtggatt tgctaacact gttgatacat tgtcagctat
     1861 caaatacgct acagttaaac caatccgtga cgaagatggc tacatctacg attacgaaac
     1921 aatcggtgac tacccacgct ggggtgaaga tgacccacgt tcaaacgaat tggcagaatg
     1981 gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa ctatacaaag acgcagaagc
     2041 tacagtatca cttttgacaa tcacatctaa cgttgcttac tctaaacaaa ctggtaactc
     2101 accagttcac aaaggtgtat acctcaacga agatggttct gtgaacttgt ctaaacttga
     2161 attcttctca ccaggtgcta acccatctaa caaagctaaa ggtggttggt tgcaaaactt
     2221 gaactcactt tctagccttg actttagtta tgcagctgac ggtatctcat tgactacaca
     2281 agtatcacct cgcgctcttg gtaagactcg tgatgaacaa gttgataact tggtaacaat
     2341 ccttgatggt tacttcgaaa acggtggaca acacgttaac ttgaacgtta tggacttgaa
     2401 cgatgtttac gaaaaaatca tgtcaggcga agacgttatc gtacgtatct ctggatactg
     2461 tgtaaacact aaatacctca ctccagaaca aaaaactgaa ttgacacaac gtgtcttcca
     2521 cgaagttctt tcaatggatg acgccttgga tgcattgagc taatcaagtt cttgaataat
     2581 aaaaaggaac cctcggtcaa acgactgagg gttttgtgct tgggatagta tgagcaattc
     2641 cttcggcgca atatgcaatg tttttgggct ctttgtcaac tgtagtgggt tgaaaaaaag
     2701 ctaagct
//
LOCUS       DAVBCR010000119         2657 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCR010000119 DAVBCR010000000
VERSION     DAVBCR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.119"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            204..2363
                     /gene="pfbA"
                     /locus_tag="VQ508_001986"
     CDS             204..2363
                     /gene="pfbA"
                     /locus_tag="VQ508_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2490929.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2347..2643
                     /locus_tag="VQ508_001987"
     CDS             2347..2643
                     /locus_tag="VQ508_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490930.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT         1036 a          326 c          500 g          795 t
ORIGIN      
        1 tagtttcagt ctactataga actgaccaag tcagtaacct agacttaggg caaggcggca
       61 ctgacctagt ttgaagagat ttccgaagag tataaatttt aatattttct tgtgttattc
      121 cttgacaatt caatttggaa aatatatgat aaagataatg acagcggtgt cattctatct
      181 attttaagaa aagtaataat caattgttaa aaatagtaaa aaaattggag gttctgatga
      241 aatattttgt tcctaatgag gtattcagta ttcgtaaatt aaaggtgggg acttgctcgg
      301 tactattggc aatttcaatt ttgggaagcc aaggtatttt atcggatgaa gttgttacta
      361 gttcttcacc gatggctaca aaagagtctt ctaatgcaat cactaatgat ttagataatt
      421 caccaactgt taatcaaaat cgttctgctg aaatgattgc ctctaattca accactaatg
      481 gtttagataa ttcgttaagt gttaatagta tcagctctaa tggtactatt cgttccaatt
      541 cacaattaga caacagaaca gttgaatcta cagtaatatc tactaatgaa aataagagtt
      601 ataaggaaga tgttataagt gacagaatta tcaaaaaaga atttgaagat actgctttaa
      661 gtgtaaaaga ttatggtgcg gtaggtgatg ggattcatga tgatcgacaa gcaattcaag
      721 atgcaataga tgctgcagct caagggctag gtggaggaaa tgtatatttt cctgaaggaa
      781 cttatttagt aaaagaaatt gtttttttaa aaagtcatac acacttagaa ttgaatgaga
      841 aagctacaat tctaaatggt ataaatatta agaatcaccc ttccattgtt tttatgacag
      901 gtttatttac ggatgatggt gcgcaagtag aatggggccc aacagaagat attagttatt
      961 ctggtggtac gattgatatg aacggtgctt tgaatgaaga aggaactaaa gcaaaaaatc
     1021 taccacttat aaattcttca ggtgcatttg ctattgggaa ttcaaataac gtaactataa
     1081 aaaatgtaac attcaaggat agttatcaag ggcatgctat tcaaattgca ggttcgaaaa
     1141 atgtattagt tgataattct cgttttcttg ggcaagcctt acccaaaacg atgaaggatg
     1201 ggcaaatcat aagtaaggag agcattcaga ttgaaccatt aactagaaaa ggttttcctt
     1261 atgccttgaa tgatgatggg aaaaaatctg aaaatgtgac tattcaaaat tcctattttg
     1321 gcaaaagtga taaatctggg gaattagtaa cagcaattgg cacacactat caaacattgt
     1381 cgacacagaa cccctctaat attaaaattt taaataatca ttttgataac atgatgtatg
     1441 caggtgtacg ttttacagga ttcactgatg tattaatcaa aggaaatcgc tttgataaga
     1501 aagttaaagg agagagtgta cattatcgag aaagcggagc agctttagta aatgcttata
     1561 gctataaaaa cactaaagac ctattagatt taaataaaca ggtggttatc gccgaaaata
     1621 tatttaatat tgccgatcct aaaacaaaag cgatacgagt tgcaaaagat agtgcagaat
     1681 atttaggaaa agtatcagat attactgtaa caaaaaatgt aattaataat aattctaagg
     1741 aaacagaaca accaaatatt gaattattac gagttagtga taatttagta gtctcagaga
     1801 atagtatatt cggtggtaaa gaaggaattg ttattgagga ttcaaagggt aaaataaccg
     1861 ttttaaataa ccaattttat aatttatccg gtaagtatat atcattcatc aaatctaatg
     1921 caaatgggaa agaatctgtt atacgtgata gcgatggtaa tttcaatatt gtaacggaga
     1981 atgggcttta caaaattgta acaaataatt taagtgataa aaacgaaaaa gaaaaaaaca
     2041 aagaggaaaa acaatctaat tcaaataatg taattgatag taaccagaag aacggagagt
     2101 ttaactcaag taaagataat agacaaatga atgacaagat cgacaataaa caagataata
     2161 agacagaaga agtaaactat aaaatagttg gagatggcag agaaactgaa aatcatatta
     2221 ataaatctaa agaaatagta gatgtaaaac aaaaattacc aaagactggt tcgaacaaga
     2281 ttatggaact attcttaaca gtgacaggaa ttggtttact tttgacacta aaagggttga
     2341 agtattatgg taaagataaa taaaatttgt tcgatacaag gaagttccgt agaaaatgag
     2401 gatattgtag gttcacaaaa tcagtatttt tggattattg gtggagctac agatttatat
     2461 aattctaaag aggagatagg ttattcagtc tcagaagttg tacatatttt atcagaaagt
     2521 ttgtcggtta attgtaaaga atcaaaaact cttaaacaaa tatttgaaac tgctttactc
     2581 gaggttaaag atgaaatcgg tttaaactca tatgaattaa cagagtatag taaaatgaaa
     2641 taataatagg acaaatc
//
LOCUS       DAVBCR010000120         2612 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCR010000120 DAVBCR010000000
VERSION     DAVBCR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.120"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            76..993
                     /locus_tag="VQ508_001988"
                     /pseudo
     CDS             76..993
                     /locus_tag="VQ508_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ508_001989"
     CDS             complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ508_001989"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2490931.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2109..2390)
                     /locus_tag="VQ508_001990"
     CDS             complement(2109..2390)
                     /locus_tag="VQ508_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2490932.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2455..2577
                     /locus_tag="VQ508_001991"
                     /pseudo
     CDS             2455..2577
                     /locus_tag="VQ508_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          695 a          605 c          475 g          837 t
ORIGIN      
        1 gtgcatcagt tagttgttta cttgcttcat cattcataga actactatgc catattttgt
       61 ttcgcaggaa gtctgatcat ttcaaatcca gccattgctc taggagctca agcgaccttt
      121 atcctattga atatcttcat tgtatcacgt ggggttcaaa aagggattga aagagcttcg
      181 aaagtcatga tgcccctgct ctttatcatc tttgtcgtca tcatcgaacg ctctctcagt
      241 ttgccaaatg ccatggaagg ggttctttac ttcctcaaac cagatttttc aaaactgact
      301 agcgctggtc tcctctatgc tctgagacaa tctttctttg ccctctcact aggggttaca
      361 gccatgttga cctatgcttc ttacttggac aagaaaacca atctggtcca gtcaagaatc
      421 tccatcgtag ccatgaatat ctcggtatcc atcatggcag gtctagccat tttccaagct
      481 cggtccccct tcagactgaa tattgaaggg ggaccgagct tggtctttat cgtcttgcct
      541 caactctttg acaagatgcc ttttggaacc attttctatg tcctcttcct ctttgcgact
      601 gtcacttctt ctgtcgttat gctggaaatc aatgtagaca atatcaccaa tcaggacaac
      661 agcaagcgtg ctaaatggag tgttatttta ggaattttga cctttgtctt tggcattcct
      721 tcagccctat cttacggtgt catggcggat gttcacattt ttggtaaagc cttctttgac
      781 gctatggact tcttggtttc caatctcctc atgccatttg gagctctctt cctttcactt
      841 tttacaggct atatctttaa aaaggctctt gcaatggagg aactccatct cgatgaaaga
      901 gcatggaaac aaggactgtt ccaagtctgg ctcttccttc ttcgtttcgt cattccaatc
      961 atcatcgtgg tcttcatcgc ccaatttatg taatcaaaag gacttgagta atgaactcag
     1021 gtcctttctt ttttatggat gactaacaat taattctaaa tcttgccctt ctagagtcca
     1081 agattcaaca tcacttggta ggataaagtg tctccctttt tggattggat agtttttccc
     1141 gtcaacagtt agttgacctt gaccagccaa gacactcaac aagctgtagt cagctgtctt
     1201 ttcaaagtta acttttccag taatttccca tttgtaaact gcgaagaaat cattggatac
     1261 aaggatagtg gaacgtagat catctgcttt gattgtcact ggacggctat ttgcgggttc
     1321 accaatgttc aagacatcga tagatttttc aaggtgaagt tcacgtaaat tgcccttgtc
     1381 atccttacgg tcaaagtcat agacacggta agtggtatcg ctagactgct gggtttcaag
     1441 aatcaagata cccgccccga tagcgtgcat ggtaccgctt ggtacataga agaaatctcc
     1501 tgctttaact ggcacttttg tcaacagcgc atcccagttc ttatcctcga tttgctggcg
     1561 gagttcttct tttgacttgg cattgtgacc gtagataatc tctgaacctt catctgctgc
     1621 aatgatgtac caacattctg tttttccaag ttcgccttca tgctcgagtc cataagcatc
     1681 gtctgggtga acttggacac tgagccagtc gttggcatcg aggatcttgg tcaaaagtgg
     1741 aaatacaggt tctggacgat tgccaaataa ttcacggtgt tccgcataca aagtagcaag
     1801 atctgttccc tcgtaacgac cattggcaac tttagaaaca ccatttagat gagctgagat
     1861 agcccagtac tctccgactt tttcacttgg gatgtcgtag ccaaactcat cacgtagctt
     1921 ggtaccaccc cagatttttt cttgcataac tgattgtaaa aataatggtt ctgacatctt
     1981 aatctcctgt ctgatttttc tcccctcatt atagcaaaaa aagagttcca tttgaactct
     2041 tttttacatc ttataaagca gggagaagat tttataaaaa tagtaaacaa atgtgctcta
     2101 ctcgatgctt acaccattgc tataaatgac atgtaccaat agaaggactt cttcttgcta
     2161 cgtttgagag tcccatttcc tacattatct cgatctacat agataaagcc atagcgctta
     2221 ttcatttccc ctgtgccagc tgaaaccgaa tcgatacagc cccatgtcgt ataaccaagc
     2281 aagtcaaccc cgtctttgta aatggcatct cgcatggcct tgatgtgggc ctctaagtaa
     2341 gtaatccgat agtcatctgc tacataacca ttctcatccg gtgtatccat agcaccgagt
     2401 ccattttcta cgataatact atactaaaat caaaaagcat tatataatag tgatatgaaa
     2461 tcaactaaag aagaaatcca aaccatcaaa acacttttta aagactctcg tacagctaaa
     2521 tatcataaat gccttcaaat cattctattt cgtctgatgg gcaaatctta taaagactcc
     2581 gactaatatt ggttttgcat ttattcctcc at
//
LOCUS       DAVBCR010000121         2599 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCR010000121 DAVBCR010000000
VERSION     DAVBCR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.121"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            213..527
                     /locus_tag="VQ508_001992"
     CDS             213..527
                     /locus_tag="VQ508_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490933.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            537..743
                     /locus_tag="VQ508_001993"
     CDS             537..743
                     /locus_tag="VQ508_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2490934.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            743..940
                     /locus_tag="VQ508_001994"
     CDS             743..940
                     /locus_tag="VQ508_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490935.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            943..1665
                     /locus_tag="VQ508_001995"
     CDS             943..1665
                     /locus_tag="VQ508_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2490936.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            1677..2318
                     /locus_tag="VQ508_001996"
     CDS             1677..2318
                     /locus_tag="VQ508_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490937.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2336..2566
                     /locus_tag="VQ508_001997"
     CDS             2336..2566
                     /locus_tag="VQ508_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490938.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          763 a          430 c          527 g          879 t
ORIGIN      
        1 aaccatacct acggcgaggt gaagctgacg tggtttgaag agattttcga agagtattaa
       61 tgatagataa actggtcagt aacctactcc tgaccttttt cttttgcaaa atgacaaaaa
      121 tcataaactt tttgacaact atacttgtca aaaagaaaaa gatgtgttac aatgaattca
      181 agttaagaaa taggaagcag aaaggagtta taatgtgggt actagtattt atactattta
      241 tgattttctt ttattctaat aattctaaaa aaatcaagaa actagagaat aaaatcaaaa
      301 aacttgagcg aaaagagaaa ggaaacgcag aaatgtcgag attattgcaa gaaatgattg
      361 gaaaggaacc aattataacg ggagtgtata ttgggccaga taactgggaa gttgtggatg
      421 ttgatgagga atgggtaaag ctacgacgtg tagataatac gggaaaagaa aaattcaagt
      481 tgcaacgcat tgaggatatc caaaccgttg aatttgacgg agagtaggtg tgtaacatgc
      541 aacttaaaaa tcgtctaaaa gagcttagag ctcgcgatgg tctcaatcaa accgacctag
      601 ccaaactggc aggggtttcc agacagacca ttagcctact agaacgggat gagtacaccc
      661 catccattat cattgccctg aaaatatctc aaattttcaa cgaaacagtc gaatcggtat
      721 ttcgtttgga ggaggacgag tgatgaacaa gtataaagtg atttattatg tagtggtcat
      781 agctttatta gtcagtgtat ttctactgat tgggatggac ctaagctggt ttaatcccta
      841 tcaaagcgac caatttgttt gggtctactt tgctctcatc ccagtaattg aatggattga
      901 aaagaaatcc aaaaatctag caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga
      961 ttgttatttt tgatgtctat tgtcttgggc atgtttgtag ggatgtttaa ggcacgtgtc
     1021 gaatcccacg aaattatttt agatgtaaaa gccttgatgc catggatatc agctatttgt
     1081 ttactgatag gtttcattag tatgtttttg actttcaatt tcttaaagaa aagcagaaaa
     1141 tttcattcct tgtatcaaga ggaaatggat gacgatctga atgaaaccta ttatgtgcaa
     1201 atgtatcgga atcttgagtt tggaaccatt gcttttaata ttacaggtgt agcgattcca
     1261 ttggctattt ttatttcatt aagtgaggtg attatattgc atacaaaccc tcaaacattt
     1321 ttcctttctt tcttactctt tgtggtattc ttagtcgctc aaaaatctct ttttaaaacc
     1381 attgcgattg ttcgtcagtt tgatttagaa tttttcgcta caccaaagga tgtcttgaac
     1441 tatataaatt cttatgatga aggggagcgt caggctaatt tggaacagag ttttcgaatt
     1501 ttattccaat tacaccaata tgtcttacca gccttatata tttttcttat tatcatttct
     1561 ttcttgacag gagagattca gttactagct ttcttgcttg taggagccat ccatgtttat
     1621 atcaatgtga tgcagttacc tatggtaaaa cgttatttca aataaaggag ttatctatga
     1681 aattacttaa aaaccttggc tggattcttc tagcccttct atccttttta tttatctatg
     1741 gctttattca ggggctcgcg actgcgtcgc ttgctttagg cgcttcaccc tatgctgtta
     1801 ctctactcta tgtggccttg gctggagtat atgtgtacgg catttacaaa tggtatcaga
     1861 aggctcctgt tcatattgag aagagcggct ttaatcgatt tatttggctt cctgtcttgg
     1921 tttggttctt atctctggtc gttcaattct tcttgccaga tgatccttca gtaaatcagc
     1981 aaatagcgac agacttgacc ttgtctcaac cacttttctc attctttgct gtggttattt
     2041 ttgctccttt gacggaagag attgttttta gagggatgtt agcacgctat ctctttccta
     2101 agcaggacaa tagtaaacga accctgattt ttcttctggt atctagtctt ctatttgcct
     2161 tgattcattt tccaggtgat gtgcaacaat tttttgtcta ttttagcctt ggttttagtt
     2221 tgggtttggc ttacattagc agaaaaggtc tggtctacag tatttctctt cacgctttga
     2281 ataatttagt cggctttttg atgattctca tgctataata gagtcaggag gtcacatgaa
     2341 acgagtaatt ttattagcag tgattcaggc agtcgttcta tttttcatca ttggagcgct
     2401 agcttatgcc ttcaaaggcg atttcttcta taactatcta gcagttgtct ttgctcctat
     2461 tgcaggtgta ctgcgttttg ggacggctta cataacggaa attgtcttgc ctcgaaaggc
     2521 agccgaaatc gctgaaaagc gtaaagcagg caaaaattca aaataaaaga gtccggtgaa
     2581 gttcatcgga tttttgtat
//
LOCUS       DAVBCR010000122         2519 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCR010000122 DAVBCR010000000
VERSION     DAVBCR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.122"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(173..838)
                     /locus_tag="VQ508_001998"
     CDS             complement(173..838)
                     /locus_tag="VQ508_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2490939.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(852..2231)
                     /locus_tag="VQ508_001999"
     CDS             complement(852..2231)
                     /locus_tag="VQ508_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2490940.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT          825 a          571 c          471 g          652 t
ORIGIN      
        1 cccgcagcta gtttcctagt ttgatctttg attttcattg agtattactc tctcttgtca
       61 cttccttcta ttttaccata aagtccagcc tttgaagaac ttttactaga agacaagggg
      121 cttctgtctc tatttgccat cttgggcatc aaaaaagagg ggtcatccct ctttacgaat
      181 tcaatgctac tagggtatcc aaatactggt tgttgatgac tgccaaaata taggtatctg
      241 ctttcaagag gtcatctggt ccaaattcaa catccaatgg ggaattttcc tgctctcgga
      301 aacccaaaat attcagattg tatttgccac ggaggtctaa tttactcaga ctttgacctg
      361 cccaagactg aggaattttc atctccacga tagacacatt tttatccaac tgaaagacat
      421 caacactatt atggaaaaga atggtctgtg ctagagactg ccccatttca tactctggcg
      481 agataaccga gtcagctcca atcttttcta gcactttctt agcggtctga cttttgacct
      541 tagcaataac agtcggtacc cccaaactct tacagtgcat aaccgcaagc acactcgact
      601 ccagattttc acctgtcgcg actacaacgg tatcgcaggt atcaatccct gctgatctca
      661 ataattcttc atctgtgatg tcaccaatca ctccacgcgc caaaactggc tcaaactgat
      721 tgatgcgctc tgcgtggtca tcaatagcga taatattcat atcctgcttg gctagggcag
      781 ctaggacact gctcccaaaa attcccaagc ccaaaattcc aatcgtacga tctgacatgc
      841 tctttccttt cttaaccaat actaatatct gctttcatat agtgaatcat atctttcttt
      901 tctggatggt aatctgccaa gctaacaaac aaggtcaagg gaccaattcg tcccataaac
      961 ataagtggca tgataacact gagagccaat ttcccaaggt caggagtcag atttgccgtt
     1021 acaccaactg tactaagagc tgaaatggtt tcaaatatga ggtggataaa gggaggattg
     1081 cctttggctg ttatccctag cagaatcaat cctatcaaga agctcatcaa aaagataata
     1141 aagacactaa aggatttttg aaccgttcgc ggcgcaatcg ttcgtctcgc aacattggca
     1201 tgaggcaagc ctagaagctc acttcgtgca aagaccaaga ggacaaaaaa tgtcgtaatc
     1261 ttgagtcccc cagctgttcc tccaggtgcc ccacctagaa acatctgtaa gatataaatc
     1321 aaaagagtca caggatgagc ctgagtataa tctatcgtag aaaagccagc tgttcgcatc
     1381 gtcactgttt gaaaaaagct aactaaaacc ttatcggcaa cagggagatt gccaatcgtt
     1441 ccagcattgt tccactcaag aaagagagta gttgccgttc caaataacaa caaacctata
     1501 gtcaacaata gtacaagctt cgtatgaaag tgcagacgtc cttttttctt tcttcctaca
     1561 tgaccagcca aatcaaacca gaccataaaa ccaaggccgc ctgtaataat caagcctgca
     1621 atcaccagat tgaccagtaa atcggtctga aaagcaaata aactggtgct ccctaaatta
     1681 tcaaaaccgg cattacagaa ggctgagatc gctagaaaaa tggaactaaa aagaccacgt
     1741 ccccagccaa gttgaggaat aaggcgaaaa ctaagcaaaa tagctcccaa gctctcaacc
     1801 aaaaaggtcg tgagaaaaat agaatagaca aactttctca aagatcgagt ttctccataa
     1861 ctaaaactat cctgaatagt tgcacggcta cgaagactaa gcttttgctt gctctggata
     1921 tagaaaaccc caataaaggt catgagccct agaccaccga tctgaatcaa gagcaaacag
     1981 attatttggc cccatatatt ataggtgtga gctactggaa gggttgagag acctgtcaca
     2041 cagactgcag agacagcagt gaaaagatga tcaaaataag tcgctcgtga gctttcaact
     2101 tggacaaagg gcaagctcaa aagaagagag cctagtaaaa tgaccaaggc aaaactcaaa
     2161 aaaatacgac gggctggcga taaacgagat aagcctagct ctattttttc cttagataat
     2221 ttgaataaca tgtttccatt gtaccataaa aagttcgaaa tgacttagat aggataaggc
     2281 aaaatctact aacaccaagc ctaaaaaatg ccaccttttt attgatagct taggcaagaa
     2341 caatacttga tagatgtgac tataggtcaa aaaagcaaga caggagcttt taacgccagc
     2401 tactctatat gcttttcagg agaaaatttc tctttttgat tataggcaat gagaaccaag
     2461 gcatcgatta gaaagctagt cacagattgc tcaaaacact gttttgaggt tgcaaatag
//
LOCUS       DAVBCR010000123         2376 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCR010000123 DAVBCR010000000
VERSION     DAVBCR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.123"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..1545
                     /gene="phtA"
                     /locus_tag="VQ508_002000"
     CDS             <1..1545
                     /gene="phtA"
                     /locus_tag="VQ508_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2490941.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGDHYHFIPYSQMSELEER
                     IARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVG
                     EGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDK
                     AYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPER
                     LGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGK
                     DSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPY
                     MVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDE
                     RPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLK
                     EAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSV
                     SKEKIN"
     gene            1703..>2376
                     /locus_tag="VQ508_002001"
     CDS             1703..>2376
                     /locus_tag="VQ508_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2490942.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASE"
BASE COUNT          835 a          430 c          481 g          630 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aacagctaga
       61 ggtgttgcag tgccacacgg agatcattac cacttcatcc cttactctca aatgtctgaa
      121 ttggaagaac gaatcgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaaggc cagaacaacc aagtccacaa ccgactccgg aacctagtcc aggcccgcaa
      241 cctgcaccaa atcttaaaat agactcaaat tcttctttgg ttagtcagct ggtacgaaaa
      301 gttggggaag gatatgtatt cgaagaaaag ggcatctctc gttatgtctt tgcgaaagat
      361 ttaccatctg aaactgttaa aaatcttgaa agtaagttat caaaacaaga gagtgtttca
      421 cacactttaa ctgctaaaaa agaaaatgtt gctcctcgtg accaagaatt ttatgataaa
      481 gcatataatc tgttaactga ggctcataaa gccttgtttg aaaataaggg tcgtaattct
      541 gatttccaag ccttagacaa attattagaa cgcttgaatg atgaatcgac taataaagaa
      601 aaattggtag atgatttatt ggcattccta gcaccaatta cccatccaga gcgacttggc
      661 aagccaaatt ctcaaattga gtatactgaa gacgaagttc gtattgctca attagctgat
      721 aagtatacaa cgtcagatgg ttacattttt gatgaacatg atataatcag tgatgaagga
      781 gatgcatatg taacgcctca tatgggccat agtcactgga ttggaaaaga tagcctttct
      841 gataaggaaa aagttgcagc tcaagcctat actaaagaaa aaggtatcct acctccatct
      901 ccagacgcag atgttaaagc aaatccaact ggagatagtg cagcagctat ttacaatcgt
      961 gtgaaagggg aaaaacgaat tccactcgtt cgacttccat atatggttga gcatacagtt
     1021 gaggttaaaa acggtaattt gattattcct cataaggatc attaccataa tattaaattt
     1081 gcttggtttg atgatcacac atacaaagct ccaaatggct ataccttgga agatttgttt
     1141 gcgacgatta agtactacgt agaacaccct gacgaacgtc cacattctaa tgatggatgg
     1201 ggcaatgcca gtgagcatgt gttaggcaag aaagaccaca gtgaagatcc aaataagaac
     1261 ttcaaagcgg atgaagagcc agtagaggaa acacctgctg agccagaagt ccctcaagta
     1321 gagactgaaa aagtagaagc ccaactcaaa gaagcagaag ttttgcttgc gaaagtaacg
     1381 gattctagtc tgaaagccaa tgcaacagaa actctagctg gtttacgaaa taatttgact
     1441 cttcaaatta tggataacaa tagtatcatg gcagaagcag aaaaattact tgcgttgtta
     1501 aaaggaagta atccttcatc tgtaagtaag gaaaaaataa actaatgaaa aatgaaagtc
     1561 tcgataaaga ggctttcatt tttattatgt atatatgtaa aattcttgac aagcaatatt
     1621 aaaaagagta aactattaac tagttaatta accggtttat tactttatag tgaatcaaat
     1681 atacttaaga aaagaggaaa gaatgaaaat caataaaaaa tatctagctg ggtcagtagc
     1741 tacacttgtt ttaagtgtct gtgcttatga actaggtttg catcaagctc aaactgtaaa
     1801 agaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa cggagaattt
     1861 gactcctgat gaggttagca agcgtgaagg aatcaatgcc gaacaaatcg tcatcaagat
     1921 aacagaccaa ggctatgtca cttcacatgg ggaccactat cattattaca atggtaaggt
     1981 tccttatgac gctatcatca gtgaagaatt gctgatgaag gatccaaact accagctaaa
     2041 ggacgaggat attatcagtg aaatcaaggg tggttatgtg attaaggtag atggaaaata
     2101 ctatgtttac cttaaggatg cagctcatgc ggataatgtc cgtacaaaag aagaaatcaa
     2161 tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg acttcaacaa acgatggtgc
     2221 ggtagccttt gcacgttcac aaggacgcta tactacagat gatggttata tctttaatgc
     2281 ttctgatatc attgaggaca cgggtgatgc ttatatcgtt cctcacggcg accattacca
     2341 ttacattcct aagaatgagt tatcagctag cgagtt
//
LOCUS       DAVBCR010000124         2318 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCR010000124 DAVBCR010000000
VERSION     DAVBCR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.124"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(30..426)
                     /locus_tag="VQ508_002002"
                     /pseudo
     CDS             complement(30..426)
                     /locus_tag="VQ508_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(371..553)
                     /locus_tag="VQ508_002003"
     CDS             complement(371..553)
                     /locus_tag="VQ508_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2490943.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            910..>2318
                     /gene="pcpA"
                     /locus_tag="VQ508_002004"
     CDS             910..>2318
                     /gene="pcpA"
                     /locus_tag="VQ508_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2490944.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKG"
BASE COUNT          811 a          351 c          379 g          777 t
ORIGIN      
        1 agccttcata gcgtggcatt tctttttact tccgtcacat caatcattac cgtgtcctca
       61 gaactgagag gagttcttga aatcgtaaca ccacctttga acaagagtta cttcaaccca
      121 ttagctccga cggattaagt tgctttcgtg aataccaaaa tcagccgcaa tttcttcata
      181 agtgcggtat tctcgcacat attgaagagt ggccataaga aggtcttcta ggcttaattt
      241 aggttttcgt ccaccttttg tgtgtttaag ttgataagct gtttttaaca cagctaacat
      301 ctcttcaaaa gtggtgcgct gaacaccaac aagacgctta aatcgtgcat cggttagttg
      361 tttacttgct tcataatttc gcagggagtc tattgactct ttggtaggtg tcaatgtttt
      421 tttcatctat cccgagaatt attttcccgc catttgtatt tgcaaatgct gagtaggttt
      481 cccagaaaga ctctggaaga ttgtttttag cttttttgta ttctaaatca accccttcaa
      541 attttaagtc catatttttc ctttacatct gttttttgtg gttctggtat ttgttcaagt
      601 tgagtgataa tatagcgaat tgaatttcga gagtttttac tcagttaatt tcttttttaa
      661 cccactttaa tttctttttt aacccacttt aatttctttt ttaacacggg ttaaaaaaga
      721 aattaaagtg ggttaatttt tcttgactta aaatttaaaa atgatatact gtgtttatgg
      781 agagatgaca atttataaaa tgtactactc tattctacta gataatgatt gaataaaact
      841 ttttataaca acggctagca aagttataat tttatatcta ttagaaaata aatatgtaag
      901 gaaatttaaa tgaaaaaaac tacaatatta tcattaacta cagctgcggt tattttagca
      961 gcatatgtcc ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag
     1021 actaaagttg gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa
     1081 ggtaacatag gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt
     1141 caagatggaa aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc
     1201 gttactgaag tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc
     1261 tactatccta gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc
     1321 catggaagta aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa
     1381 gatggagctt ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat
     1441 attggaacaa gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt
     1501 tcaaaattag aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta
     1561 acattaccaa aatcggttaa aacattagga agtaatctat ttagactcac tactagctta
     1621 aaacatgttg atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt
     1681 tcaaaagata aaacccaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa
     1741 acgcctaagg agacaaaaga acttgcatca tattcgttta ataaaaattc ttacttgaaa
     1801 aaactcgaat tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc agatgcgatt
     1861 aaacttgaag aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac
     1921 ggtaatttag aattaaaaga acttatatta ccagataatg ttaaaaattt tggtaaacac
     1981 gttatgaacg gtttaccaaa attaaaaagt ttaacaattg gtaataatat caactcattg
     2041 ccgtccttct tcctaagtgg cgtcttagat tcattaaagg aaattcatat taagaataaa
     2101 agtacagagt tttctgtgaa aaaagataca tttgcaattc ctgaaactgt taagttctat
     2161 gtaacatcag aacatataaa agatgttctt aaatcaaatt tatctactag taatgatatc
     2221 attgttgaaa aagtagataa tataaaacaa gaaactgatg tagctaaacc taaaaagaat
     2281 tctaatcagg gagtagttgg ttgggttaaa gacaaagg
//
LOCUS       DAVBCR010000125         2233 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCR010000125 DAVBCR010000000
VERSION     DAVBCR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.125"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>2233
                     /locus_tag="VQ508_002005"
     rRNA            <1..>2233
                     /locus_tag="VQ508_002005"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          613 a          458 c          665 g          497 t
ORIGIN      
        1 gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag ttgaagagac
       61 ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt agacccgaaa
      121 ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga ggaccgaacc
      181 agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc aaacgaactt
      241 ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta gagattcttg
      301 gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca gataaactcc
      361 gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg tagtcgaaag
      421 ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga aaaggatgtg
      481 gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa agagtgcgta
      541 atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg
      601 aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat gaaggtatac
      661 cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg aaagacaggt
      721 gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg ccctgggtta
      781 gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg atattcctgt
      841 actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg attggaagag
      901 tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac attgagctgt
      961 gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga aaagcttcta
     1021 gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg cgagtagcct
     1081 caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag
     1141 aaggggtgct gactttaagt cagccgcagt gaataggccc aagcaactgt ttatcaaaaa
     1201 cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc cggtgctgga
     1261 aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag taaacggcgg
     1321 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac
     1381 gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg
     1441 tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta ctgcagtttg
     1501 atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat cgggacgcca
     1561 gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct aacccagata
     1621 ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa
     1681 aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc gcagagtgta
     1741 aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa agtcgggctt
     1801 agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc taccctgggg
     1861 ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca cctcgatgtc
     1921 ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag
     1981 cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg
     2041 taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact taccgctggt
     2101 gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg ataaacgctg
     2161 aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat atatcagtaa
     2221 gagccctgag aga
//
LOCUS       DAVBCR010000126         2223 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCR010000126 DAVBCR010000000
VERSION     DAVBCR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.126"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ508_002006"
     CDS             101..1078
                     /locus_tag="VQ508_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2490945.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ508_002007"
     CDS             1075..2157
                     /locus_tag="VQ508_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2490946.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          703 a          502 c          402 g          616 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt gttggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacaa aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgacca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattgactg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg
     2041 ggcaaggaag gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattttatac
     2221 tca
//
LOCUS       DAVBCR010000127         2213 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCR010000127 DAVBCR010000000
VERSION     DAVBCR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.127"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(275..883)
                     /locus_tag="VQ508_002008"
     CDS             complement(275..883)
                     /locus_tag="VQ508_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2490947.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            1049..1219
                     /locus_tag="VQ508_002009"
                     /pseudo
     CDS             1049..1219
                     /locus_tag="VQ508_002009"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            1208..1819
                     /locus_tag="VQ508_002010"
     CDS             1208..1819
                     /locus_tag="VQ508_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2490948.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT          863 a          227 c          338 g          785 t
ORIGIN      
        1 acttagttct gtgcagtaca gaactaaatc ctttggataa attatttgtc taattttttg
       61 gggtcagtac agtattaaat accatgatct tatgcgtttt ataataaaaa atctgttctt
      121 gttttatttt tagaaaattt actaaagact aaaaatatta tgaataattt aaaaaatagt
      181 caggttatat agttttttga aattaaaatt attttatcgt tgaaattagt taaaaatttt
      241 tataaaatca agatgcaagg ctatgagatg ttctctagtt taatttttca tacttcgaga
      301 atataaagtt atataaaaat gcattagcat attttgttat tgagggaagt ataaaaatca
      361 ttaaagttat ccaaactagt aattgattct gtaatataaa gaagagtaga actgggataa
      421 tttcaataat tctaataaga acggcaataa aaaaatgttt ataggctaac atgattgaat
      481 tgtgtagaga attttttaaa gtgttattat atctaccgat taaaggaatt agaaatgttg
      541 ttgctattaa atgaagtatg atatatatta ctccaatatg tcgtaagata gaaatgatat
      601 atataggtaa tggtatgtag ggtaggaaga acaaactgaa taataagatt aaaattggta
      661 aattagatat taatgctgct cctaaatttt tccaataatc tttaaaaaaa ctgtttgcga
      721 agtctctata gggataggat tcctcgtgtc gtacaatttt tattagagtt gctattaggg
      781 cactttcacc tgatccgata gcaataactg ttactactga cgctacatag agtagattta
      841 acattataag atttgaaact ttatcaataa attttatctg catctttaac tcctgctgtt
      901 tttatactta taatatcaca tcatttgagt ttcatcaact ttgaatacat taagaaagaa
      961 tataactatg aggaaaggaa aaaattatta tgaataaatc caaaaaatat aaagaaagaa
     1021 ttcattatga atacgataat cttactaaat cagagaaaaa attgctgact atattttagt
     1081 tagagatagt gaaatagttt atgaaacgtc ttcagaaatt gttagtaaag taaaagttgg
     1141 agagggaact cttaatcgat tttgcaaaaa gattggatac tctggttttc aggaactaaa
     1201 attaaaaatg acgaaagata tattagagtt agaatctcaa aaaatgagta gtgacacatt
     1261 tattgatgaa ataaaaaata attatttatc tattgtggaa agtacaagaa aattaattga
     1321 tggtagacaa atagaattag ctataaaatt aataagagaa gcaaaccaga ttttaatgat
     1381 tggtgtgggt tctagtggaa atgcggctag agaatttgag agtagtttgt taagaattgg
     1441 aataataagt aagacggtaa ttgataccca ttttcaactc atgcatactg cgttattgaa
     1501 agataatgat ttgataatag cgtttagttt atctggttcc acaaaagaag ttgaggaaac
     1561 tttattaaac gcaaaaagaa aaaatgttaa aattatttca ataacaaatt attcgtccag
     1621 aaatatagca aaattatctg actgcgttct tttgacatca aaaaaagaat cttatttaga
     1681 gggtggtagt ttaatggcta aggcttctca actattcata attgatgtaa tatgcactag
     1741 actctcttta ataaattatg aagatactat atgtaaaaaa gaagagattg cttctttgct
     1801 gtcaaataaa gtggaataaa tcaataattt ttaaatttta ataaaaattt tttaaaataa
     1861 attaaaaaac gatcctaaaa gggtcgtttt tttgctaaag ttttaaaaat aaacaataat
     1921 taagattata gtgcgttgaa tctatattag gacataacaa ttcttaaatc attgttagaa
     1981 attagtttga attttccaat caatttgttc gatttttaca tcattgcatt atatcacgtg
     2041 actattaacc ttgttttctc ctaaaattga ttttcttctt ttcttatctt gtccactcga
     2101 aacaagtatt gtaagaattt gattattttt gaaagtactt ttaatatatt tgagatagtt
     2161 aaaaaagatt tgaaactaaa ttccaaatta gaaaaagact tgaaatgctt aaa
//
LOCUS       DAVBCR010000128         2161 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCR010000128 DAVBCR010000000
VERSION     DAVBCR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.128"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            42..1103
                     /gene="dinB"
                     /locus_tag="VQ508_002011"
     CDS             42..1103
                     /gene="dinB"
                     /locus_tag="VQ508_002011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2490949.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(1250..2095)
                     /locus_tag="VQ508_002012"
     CDS             complement(1250..2095)
                     /locus_tag="VQ508_002012"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2490950.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          726 a          438 c          435 g          562 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaaa aggtgctagg catgttgatt tttcctttgt
       61 taaatgattt gtcaagaaaa atcatccata ttgacatgga tgcctttttt gctgcagtgg
      121 aaatcaggga taatcctaaa ctcagaggaa aacctgtcat tattggaagc gaccctcggc
      181 aaacaggtgg acggggagtc gtttctacct gtagttatga ggcaagagct tttggtgtcc
      241 attctgccat gagttccaag gaagcttatg aacgttgtcc ccaggctgtc tttatctcag
      301 ggaattatga gaaatacaag tctgtgggac tccagattcg agctattttt aagcgctata
      361 cagatttgat tgaacccatg agcattgacg aagcctattt ggatgtgaca gaaaataaac
      421 tcggtatcaa gtcagcggtc aaaattgctc gcctcattca aaaagatatc tggcaagaac
      481 tccatctaac tgcttccgca ggcgtttctt acaacaaatt cttagctaaa atggcgagtg
      541 attatcaaaa accacatggt ttgacagtga ttctacctga acaggctgag gattttctca
      601 aacaaatgga tatttccaaa tttcatggag taggaaaaaa gacagtagaa cgtcttcatc
      661 aaatgggcgt ttttactggt gctgatttac ttgaagttcc tgaggtaacc ctaatagacc
      721 gttttggtag actaggctat gatctgtatc gaaaggctcg tggcattcac aactctccag
      781 tcaaatccaa tcgcatccgt aaatcaatcg gcaaggagaa aacctacggg aagattctcc
      841 ttgctgagga agatatcaaa aaagagctga ctcttctatc agaaaaagtc gctctcaatc
      901 tacatcaaca agaaaaagct ggaaaaattg tcattttgaa aatccgctac gaggactttt
      961 caactcttac caaacgaaaa agtcttgctc aaaaaacaca agatgctagt cagataagcc
     1021 aaatagccct gcaactctat gaagaattaa gcaagaaaga aagaggtgtc cgcctattgg
     1081 ggattaccat gactggattt taaagctcaa tgaaaatcaa agagcaaact aggaggctag
     1141 ccgcaggctg ctcaaaacac tgttttgagg ttgcagataa agctgacgcg gtttgaagag
     1201 attttcgaag agtataaaac cttgaagagt tgccccttca aggtttttct tatacaaata
     1261 aacggacaaa actgtaaagt agcaaaacac taccaagcac gatacggtat ttaccaaaaa
     1321 gggtgaagtc gtgttttttc acatagctgg tcaagaagcg aatagccacc atgctgaccg
     1381 caaaagctac tcccatcgcg accaagagca aaaacaattg cccaaagctc aagagttctc
     1441 cggctttcac aaatttgaaa atctttaagg cactagctcc aaacataaca ggaatcccaa
     1501 gatagaaggt aaattctgtc acaactgaac gactggttcc atttaacaaa ccaccgacaa
     1561 tcgttgcacc tgaacggcta gtccctggta aaagagcaag aacttggaag agtccgatat
     1621 agaaagcggt cgtataagga agcttgtcca actctgttac acttggctcg atagcacgcg
     1681 ctttattgcg cttttccaaa tagatgaagg caaccccgta gataatcaac atgagagcaa
     1741 ctgaaaccat gttatggaag tgggtatcaa accaatcatc aaatttaaag acaccaagta
     1801 aaggtaaagt agcaatcaag accttcaacc atagtctcca agtcttacga acttcctgtt
     1861 tgtccttagt cggtttaaaa ggattgagct tgttaaaata aatcaccata actgctaaaa
     1921 tagcaccaag ctgaatcacg acattaaaca tggacataaa ggcttcattt tgattttggt
     1981 attggatgaa ttcctctgct aaaatcaagt gacctgtact ggaaatcggc aaccattccg
     2041 taattccttc aacaatcccg aagaagatag attttaaaat ttcaataaga tacatagatt
     2101 actccctata ttataattag cgcagttagc tactgccaaa gcccaagtgg tatacttgga
     2161 a
//
LOCUS       DAVBCR010000129         2150 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCR010000129 DAVBCR010000000
VERSION     DAVBCR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.129"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            81..530
                     /locus_tag="VQ508_002013"
     CDS             81..530
                     /locus_tag="VQ508_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2490951.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            687..1097
                     /locus_tag="VQ508_002014"
     CDS             687..1097
                     /locus_tag="VQ508_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2490952.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1213..>2150
                     /gene="pavB"
                     /locus_tag="VQ508_002015"
     CDS             1213..>2150
                     /gene="pavB"
                     /locus_tag="VQ508_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2490953.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDS"
BASE COUNT          661 a          410 c          477 g          602 t
ORIGIN      
        1 tacggcaagg cgacgttgac gcggtttgaa gagattttcg aagagtataa gaaaaaatca
       61 gtcccctaaa ggagcagatt atgaagttat tgtctatcgc catttctagc tataatgcag
      121 cagcctatct tcattactgt gtggagtcgc tagtgattgg tggtgagcaa gttgggattt
      181 tgattatcaa tgacgggtct caggatcaga ctcaggaaat cgctgagtgt ttagctagca
      241 agtatcctaa tatcgttaga gccatctatc aggaaaataa atgccatggc ggtgcggtca
      301 atcgtggctt ggcagaggct tctgggcgct attttaaagt agttgacagt gatgactggg
      361 tggatcctcg tgcctacttg aaaattcttg aaaccttgca ggaacttgag agcaagggtc
      421 aagaggtgga tgcctttgtg accaattttg tctatgaaaa ggaaggtcag tctcgtaaga
      481 agagtatgag ttacgagtca gtcttgcctg ttcggcagat ttttgactag gaccaggtcg
      541 gaaatttctc caaaggccag tatatcatga tacactcgct gatttatcgg acagatttgt
      601 tgcgtgctag ccagttctaa ctgcctgaac atacttttta tgtcgatgat ctctttgtct
      661 ttacgcccct tcagcaggtc aagaccatgt actatctgcc tgtcgatttc tatcgttatt
      721 tgattgggcg tgaggaccag tctgtcaatg agcaagtgat gattaagcgc attgaccagc
      781 aactcaaggt caatcgactc ttggtagacc agcttgattt gtcccaagtg agtcatccta
      841 aaatgcgaga atatctgctg aatcatattg aaatcacgac ggtgatttcc agtaccctgc
      901 tcaaccgatc tgaaacagcg gagcatctgg caaaaaaacg ccaattgtgg acctatattc
      961 agcagaaaaa tccagaagtc tttcaggcta ttcgtaagac catgttgagc cgtttgacca
     1021 aacattctgt cttgccagat cgcaaactgt ccaatgtcgt ctatcaaatc accaaatctg
     1081 tttatggatt taattaatat aagtgtttta taagagggat ttaagaaaaa ttttaacttt
     1141 ttcttagtcc tttttaattt caggagatta tactagagtc atcaaataaa gaaagactct
     1201 aaggagaatc ctatgaaatt caatccaaat caaagatata ctcgttggtc tattcgccgt
     1261 ctcagtgtcg gtgttgcctc agttgttgtg gctagtggct tctttgtcct agttggtcag
     1321 ccaagttctg tacgtgccga tgtggtcaat ccgaccccag gtcaagtctt acctgaagag
     1381 acatcgggaa cgaaagaggg tgacttatca gaaaaaccag gagacaccgt tctcactcaa
     1441 gcgaaacctg agggcgttac tggaaatacg aattcacttc cgacacctac agaaagaact
     1501 gaagtgagcg aggaaacaaa ctcttctagt ctggatacac tttttgaaaa agatgaagaa
     1561 gctcaaaaaa atccagagct aacagatgtc ttaaaagaaa ctgtagatac agctgatgtg
     1621 gatgggacac aagcaagtcc agcagaaact actcctgaac aagtaaaagg tggagtgaaa
     1681 gaaaatacaa aagacagcat cgatgttcct gctgcttatc ttgaaaaagc tgaagggaaa
     1741 ggtcctttca ctgccggtgt aaaccaagta attccttatg aactattcgc tggtgatggt
     1801 atgttaactc gtctattact aaaagcttcg gataatgctc cttggtctga caatggtact
     1861 gctaaaaatc ctgctttacc tcctcttgaa ggattaacaa aagggaaata cttctatgaa
     1921 gtagacttaa atggcaatac tgttggtaaa caaggtcaag ctttaattga tcaacttcgc
     1981 gctaatggta ctcaaactta taaagctact gttaaagttt acggaaataa agacggtaaa
     2041 gctgacttga ctaatctagt tgctactaaa aatgtagaca tcaacatcaa tggattagtt
     2101 gctaaagaaa ctgtcgaaaa agctgttaaa gataacgtta aagacagtat
//
LOCUS       DAVBCR010000130         2142 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCR010000130 DAVBCR010000000
VERSION     DAVBCR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.130"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            129..1622
                     /locus_tag="VQ508_002016"
     CDS             129..1622
                     /locus_tag="VQ508_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2490954.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2142
                     /locus_tag="VQ508_002017"
     CDS             1635..>2142
                     /locus_tag="VQ508_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2490955.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT          757 a          322 c          424 g          639 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agagaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cg
//
LOCUS       DAVBCR010000131         2118 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCR010000131 DAVBCR010000000
VERSION     DAVBCR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.131"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            368..1714
                     /gene="gdhA"
                     /locus_tag="VQ508_002018"
     CDS             368..1714
                     /gene="gdhA"
                     /locus_tag="VQ508_002018"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2490956.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          673 a          384 c          401 g          660 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatc actattatat aatgattttt gattttagtc
       61 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
      121 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      181 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      241 tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc tgttttttct
      301 ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac aataaaagga
      361 gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg tgaaagctcg
      421 taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca ctttagaacc
      481 tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta ttactgagcc
      541 tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa ttcaagtaaa
      601 ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg gacttcgttt
      661 ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa tctttaaaaa
      721 tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg atcctaaagg
      781 taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat tgcaaaaaca
      841 catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac gtgaaattgg
      901 ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct tgactggtaa
      961 acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg gtttggttta
     1021 ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga aagtcgttat
     1081 ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac tcggtgcaac
     1141 tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta tcgacttcga
     1201 tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg cagctgaaaa
     1261 agcaactgca acctaccacg aaggtactgt atggacttac gctggaaact atgacattgc
     1321 tcttccatgt gccactcaaa acgaaatcaa cggtgaagca gctaaacgtt tggttgctca
     1381 aggcgttatc tgtgtatctg aaggtgccaa catgccgagc gaccttgatg ccatcaaagt
     1441 ttacaaagaa aatggtatct tctacggacc tgcaaaagct gccaacgctg gtggtgtagc
     1501 cgtttcagct cttgaaatga gccaaaacag tcttcgcctc tcatggactc gtgaagaagt
     1561 tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa cgacttcaga
     1621 aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct ttgaaaatgt
     1681 agcaaatgcc atgattgcac aaggtattgt ttaagattta tttactcaat cctcaatcac
     1741 tacgattggg gatttttagc ttgaattaaa aaattggaaa atctaattaa ataaaatgaa
     1801 atgaaataaa aactgaacaa attgattgcg gaattcaaac caatttctaa catacttcta
     1861 gaaattaatt tgacttttct aatcgatttg ttcatatctt atttcaattt actatactta
     1921 cttaaccctt tttatactca atgaaaatca aagagcaaac taggaagcga gccgcaggct
     1981 gtacttgagt acggcaaggc aaagctgacg tggtttgaag agattttcaa agagtattaa
     2041 acaaaaagaa aaaccgctac tcctaagaat agcgatttaa tcatgttttt aagctactac
     2101 tgtagttgct ctattatt
//
LOCUS       DAVBCR010000132         2024 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCR010000132 DAVBCR010000000
VERSION     DAVBCR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.132"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..233
                     /locus_tag="VQ508_002019"
     CDS             <1..233
                     /locus_tag="VQ508_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2490957.1"
                     /translation="IHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFAD
                     FVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            293..529
                     /locus_tag="VQ508_002020"
     CDS             293..529
                     /locus_tag="VQ508_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET2490958.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            526..939
                     /locus_tag="VQ508_002021"
     CDS             526..939
                     /locus_tag="VQ508_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET2490959.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            1057..2022
                     /locus_tag="VQ508_002022"
     CDS             1057..2022
                     /locus_tag="VQ508_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2490960.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
BASE COUNT          734 a          315 c          386 g          589 t
ORIGIN      
        1 ttatccatgt tttaaggaat aataattata gtcgagtgat atcaggaagt gctcagcctc
       61 agttaccaat tacaaaatta aaaaaaatac ttctccccct ccccccacta gccctccaaa
      121 atgagttcgc agactttgta gccctggtcg acaaatcaca attggcaatc caaaaatctc
      181 tggaagaact tgaaactttg aagaaatctc tgatgcagga gtattttggc tgatattctg
      241 ccattgtaat tacggtaatg atttgttata atacttcaaa ggaggaaatc agatggtagt
      301 aaaaacaaga aaacaaggaa attcaatcac cattacgatt ccaagtgaat ttaatattcc
      361 aagtggtgtt aaatacgaag cgaaattgtt accaagtggt gagattatct ttactcctga
      421 agaattgggg cagcaggttt cttatgtatc tgatgatgcc tttgacttaa atttagataa
      481 aatatttgac gaatacgacg atgttttcaa agctttggtg gaaaaatgac aatctatttg
      541 acagaaaagc aaattgaaaa aataaatgct ttagcaattc aacggtattc tccaaatgag
      601 aaaattcaaa cagttagttc ttctgcctta aatatgattg tgaacttacc agaacaattt
      661 gtctttggga agcctcttta tccaacaatt tttgataaag caacgatact atttgtccaa
      721 ttgataaaga agcatgtttt tgctaatgct aataaaagaa ctgctttctt cgttttggtc
      781 aaatttttac aattaaacgg ctatcgtttt tctgtaacgg tagaagaagc agtaaaaatg
      841 tgtgtaacca tcgcagtaga agctttaact gatgaaaaaa tgacaagcta ctccaaatgg
      901 atttctgaac attctgttag agaaaaggtc aaaaagtaac ctagtatgct ggatttgaat
      961 gagcacaaga aaataaatga acagacaata ttagaattct gtaatgcaga aactgatatt
     1021 gtctcttttt attgatgaat aagaaagtga gaaattatgg aatcaaaagt tacaattatc
     1081 atgcaagaaa tgttacctct tttaaataat gaacaattac tagcgttgag agagagttta
     1141 gaacatcatc tagtagacgg aaaaaagcag cagaagtatt cgaataataa cctgttgcaa
     1201 ctatttatta ccgccaagca ggtagagggc tgtagctcaa aaacaattcg ttattatcag
     1261 aggacgattg aaaacttgtt taatgctatt aaagagtctg tgacacaact cacaacagat
     1321 gatttaagga gttatttagc aaattaccag tctgaaaagg attgtagtaa ggcaaattta
     1381 gacaatatta ggcgtatatt gtcttctttt tttgcttggc ttgagcaaga ggaatatatc
     1441 attaaaaatc ccattcgacg gataaagaaa attaagactg agcaaaatgt gaaggaaact
     1501 tatactgatg aacatttgga aattatgcgt gataactgtg aaaatttgag agatttggca
     1561 ataatagacc tactagcatc gacaggtatg cgtgtagggg agcttgtaca gttgaatcgt
     1621 tcagatattg attttgaaaa cagagagtgt gttgtctttg gtaaaggaaa gaaggagaga
     1681 ccagtatatt ttgacgctcg tacgaaaatt catttaagaa attatcttaa cgacagaaaa
     1741 gatagtcacc ctgctctttt tgtaacgcta gttggaaaag tccagaggct tggaattgct
     1801 ggtgtagaga ttcgcttaag aaagttagga gacaaactcg gcatacaaaa ggttcaccca
     1861 cataagttca gaagaacttt agcgactaag gcaattgata aaggtatgcc tatcgaacaa
     1921 gtccaaaaac tgctaggtca tagcaagatt gacacaaccc tggcctatgc catggtcaat
     1981 caaaataatg tcaagcattc acaccaaaaa ttcatctctt aaaa
//
LOCUS       DAVBCR010000133         1651 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCR010000133 DAVBCR010000000
VERSION     DAVBCR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.133"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQ508_002023"
     CDS             100..1575
                     /locus_tag="VQ508_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2490961.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          468 a          335 c          328 g          520 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaaacta gccacaggtt gctcaaaaca c
//
LOCUS       DAVBCR010000134         1640 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCR010000134 DAVBCR010000000
VERSION     DAVBCR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.134"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            110..409
                     /locus_tag="VQ508_002024"
     CDS             110..409
                     /locus_tag="VQ508_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2490962.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            411..1181
                     /locus_tag="VQ508_002025"
     CDS             411..1181
                     /locus_tag="VQ508_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2490963.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1201..1365
                     /locus_tag="VQ508_002026"
     CDS             1201..1365
                     /locus_tag="VQ508_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490964.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          602 a          180 c          268 g          590 t
ORIGIN      
        1 agataactat tattacgaaa aaaaattctt gtgtaaaatt ctattgactt tctatattat
       61 ttttgtattt cataacgaat gtaatttaac aagtgaagaa attttaaaga tggattccta
      121 tgctattaat ttctgggaga gtaaaggggt cggtattgac aaaaaaatct tggaatttat
      181 tcaaaaaaat attacagtta ttaaaaataa aaattttttg aataaagatt ttgatattta
      241 tgttaaaaag attcataatt tattctttgt catttctact ttagtttcaa aagagcaata
      301 catggggata gtttcttccc aaatacatat gttcgcaaat aggatgggaa tttctcccac
      361 cattgaagtt accatgctag atttttttct gagggaggat tctgattaat atggaaaaat
      421 atttttttga tatttctatt tttgaaaaaa taaaaaaagt attatcatac caatctaaat
      481 attgggaaat atcgaagcct agggataagt acagtttcaa aagtcaaaat agaatatctg
      541 gctctaatag cattaaattt aaagattttg agttagatta tgaactggag tcacttggta
      601 aaatactttt aaaaaggaga tcgtttttac taaatgaatt tgataagaat gcgagttttg
      661 acaaactgat ttacttgttg aaaactgtat tctctcctac aaattgcaag tttgtatttg
      721 gagaagaggt tcctttattt agctatccta catcaggtgc gttaaattca attactcctt
      781 ttgtttatat tgataatatg gatgaagtaa agaatggtat atatgaagta aagtatgacg
      841 gacttgaatt taaaaaaagt atgaatatta tagacttaga aacatttacc ccaataacaa
      901 gtaaaaacag tgacgtacaa aataattcat ttaaaaaggt aaatatgata attatgttat
      961 gttcgaatta tgaaaatatt ttcaataaat atggattatt agcttatcga cttttgcatt
     1021 tagaggctgg tcatattgca caaaatatcc agcttgtttc tactgcgcta ggaataaaaa
     1081 gtttaccgat gggaggttgg tttgatgata aaatagaaac ttttattaat gaaattagtg
     1141 ataaaaaaaa tatttatctc tatgcaattg cagttggata aatttaagga ggaagtgtag
     1201 atgaacgcag aggataaatt tcgcgaacta gaaaatgaaa ataaacttct aaaacttcat
     1261 aacaaggagc ttgagaatga aattcaaaat ggatggaaat tttggatatg ggtaactgtc
     1321 cctattttag gtttgatata tgctattatt caaaaaattt tttaattatg ctagtttgtc
     1381 aggttgagta gtttttgttc aaaatttaat aaactttttt caatgaagat tatctaaaga
     1441 ttgcttctca gatgagaatg gcttctctat cttatataaa ttatcttcat agagtgtcct
     1501 gttattcatg ttatcacaga atagatagtt tttttggctc tataatattt gtagtgggta
     1561 aatcccctat agatattatg gagcctattt ttgtgtagaa aaaaagtccc atatcatcta
     1621 taatgaaaag caaccaaact
//
LOCUS       DAVBCR010000135         1549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBCR010000135 DAVBCR010000000
VERSION     DAVBCR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.135"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(6..605)
                     /locus_tag="VQ508_002027"
     CDS             complement(6..605)
                     /locus_tag="VQ508_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2490965.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            1075..1374
                     /locus_tag="VQ508_002028"
     CDS             1075..1374
                     /locus_tag="VQ508_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2490966.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          582 a          296 c          244 g          427 t
ORIGIN      
        1 tgccactagg caaaaagcat actgagataa aaaatgctca tcataatagg aacaccaaga
       61 atggtctttt catgatagaa aatcgtcaaa taggctgaaa agacatcgcc aaggacaaaa
      121 ctactaagca ggctaacaaa tatgaaccct tcacgcaaaa aaggagtgtg cttagttcgg
      181 aaataatctc caaaagctag cattgttcgt ttgatattcc ctgtcataaa ggcattattg
      241 taggcaatac ccgaaacctc accaaaagca gttgtcacca gtcccataca gaaggccaag
      301 ggctgcacta gatagatatt atctacagtt tgtggcacaa aaccaataat tattgatagg
      361 attgctaaag gaatcaagga cagaataggt tttttcacaa ttctcaattt ttccttataa
      421 atcgttaata aaaagactcc cattataaac gttattaagg ttaaaagctt atccctaaca
      481 tccgaaacat tattttgtat tagctctacc gaaagaaaga caacatttcc agcttgtcca
      541 gctacaaggg tattccctcg aacaataaaa gtgtaagcat ccacatatcc agcacaaaat
      601 gtcaaaaaag tgctaacctt ttagactgac gtgatatttt tcttataggt aataacctca
      661 ttttccctcc cattgtattt tctcttagaa atattgtacc atttctttct aaaaaatcgt
      721 agactaccat ttagatttta ctattagcat aaaaataata atagacaact atttatccaa
      781 aaatagatag aggtaacatg tttgcaaaca aagcatacga acctttagta aaatcatttc
      841 catgaaacta gaatagagcc cctcttagca aaaatcatta ttttaattta tttctaatca
      901 ctccttgaca taaataactc tcaccaataa aagactatgt cttaaaaaaa tggtataata
      961 aaatcaatac ctgggcttga tggctatgct actaataaca attaggagag aaaatcaggc
     1021 acttgttaac aacaaggatt atccccttga gatgaaagga actttagaaa tcttatgatg
     1081 aacatgcaaa acatgatgcg tcaagcacaa aaacttcaaa aacaaatgga acaaagccaa
     1141 gctgaacttg ctgctatgca atttgttggc aaatctgctc aagatcttgt ccaagcgacc
     1201 ttaactggcg ataagaaagt tgtcagcatt gatttcaatc cagctgtcgt tgacccagag
     1261 gaccttgaga ctctttctga tatgaccgtt caagccatca actctgctct tgaacaaatc
     1321 gatgaaacta ctaagaaaaa actgggtgct ttcgctggga aattgccatt ctaaaaacaa
     1381 ggagctagaa caatacttgt cgataacaaa ggctaagaaa ggtgcaaaaa ttataaaaaa
     1441 cgcatagtat caagcaacct aaaactttgg gctctataat atttgtagtg ggtaaatcca
     1501 ctatagatat tatggagcct attttattgt agaaaaaaat cccataaga
//
LOCUS       DAVBCR010000136         1544 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBCR010000136 DAVBCR010000000
VERSION     DAVBCR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.136"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            432..>1544
                     /locus_tag="VQ508_002029"
     CDS             join(432..771,771..>1544)
                     /locus_tag="VQ508_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2490967.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEK"
BASE COUNT          527 a          242 c          350 g          425 t
ORIGIN      
        1 gcttttagtt gggaaaattg ttaattcatt taaattgtaa actttaggga ttctctgaag
       61 ttatggatgt cgttccaata tgtgtacgtt ggaatgttag tgcttatatg gggggaacag
      121 aatcctctct tgattgaaga caagttagcc attaggctgg tttgtctctt tgtcaactgt
      181 agtgggttga tataatagta ttagtgagtg ggataaaagt ttcatttagt ttattcagta
      241 caaatttaac gggtcaagat ttattgaact gcaccccaat agttagacag aaaaaatcta
      301 acttttgggg tgttttatta tgaaattgag ttatgaagat aaagttcaga tctatgaact
      361 aagaaaagca aggacaaagc ttcaaacagc tttcaaaaag atttggtgtg gatgtttctg
      421 gtctaaagta catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga
      481 agaatcgtca ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag
      541 gttgttcaca aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat
      601 tttggccggc acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac
      661 cagctaaaat gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac
      721 tccaggagga gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt
      781 tagaggaaag ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc
      841 aggaggattt cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta
      901 ttatcagttg aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca
      961 gggcattgat aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag
     1021 gaatcgtggt tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt
     1081 aacggctcga attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa
     1141 agcagagaat ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac
     1201 agatgtgaca gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt
     1261 agatggtttt aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca
     1321 agtgaagagt atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca
     1381 tagtgaccaa ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg
     1441 aattcaagca tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt
     1501 ctttggcatt ttaaaatccg aaatgtttta tggctatgag aaga
//
LOCUS       DAVBCR010000137         1432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBCR010000137 DAVBCR010000000
VERSION     DAVBCR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.137"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ508_002030"
     CDS             84..491
                     /locus_tag="VQ508_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2490968.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQ508_002031"
     CDS             656..955
                     /locus_tag="VQ508_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2490969.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..>1432
                     /gene="tnpB"
                     /locus_tag="VQ508_002032"
     CDS             1216..>1432
                     /gene="tnpB"
                     /locus_tag="VQ508_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2490970.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRF"
BASE COUNT          492 a          281 c          253 g          406 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tg
//
LOCUS       DAVBCR010000138         1347 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBCR010000138 DAVBCR010000000
VERSION     DAVBCR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.138"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            146..280
                     /gene="rpmH"
                     /locus_tag="VQ508_002033"
     CDS             146..280
                     /gene="rpmH"
                     /locus_tag="VQ508_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2490971.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            564..>1347
                     /locus_tag="VQ508_002034"
     CDS             564..>1347
                     /locus_tag="VQ508_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2490972.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGK"
BASE COUNT          487 a          249 c          295 g          316 t
ORIGIN      
        1 gggcttgcat tcgaaaaagc tggaaacatt tgcctagagc tattgacaaa agtgaaagaa
       61 tcagatagaa tagtaaagta tgtttagtaa ctgatcactt tatagccggc taaacgaata
      121 caaaatctat gaggaggtat tcatcgtgaa acgtacttat caaccaagta aacttcgtcg
      181 tgcgcgcaaa cacggattcc gtaaccgtat gtcaactaaa aatggtcgtc gcgtattggc
      241 agctcgtcgt cgtaaaggac gcaaagtttt ggctgcataa tccaaacgaa ttctatcaaa
      301 aatcagtagg aactcgagtc tactgatttt tatttttgta aaaaagttca gtagatgcaa
      361 atggattcgg aagcgatgtt atagtagatt gaaactagaa tagtacacct ctatttctaa
      421 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttatt atttcatttt
      481 actataaagt tgaagtagct ggagatggcg cagcgacaat cgtctttaaa gatggttcag
      541 ctattacaat tccaggaaat caattggtag cacaagatcc aaaagcacaa gatagcacta
      601 aaccgactgc tgaaaaatca actgttaaag cacctgctca aagagtagat gtaaaagata
      661 taactcattt aacagatgaa gaaaaagtta aggttgctat tttacaagca aatggttcag
      721 cattagacgg agcgacaatc aatgtagctg gagatggtac agcaacaatc acattcccag
      781 atggttcagt agtgacgatt ctaggaaaag atacagttca acaatctgcg aaaggtgaat
      841 ctgtaactca agaagctaca ccagagtata agctagaaaa tacaccaggt ggagataagg
      901 gaggcaatac tggaagctca gatgctaatg cgaatgcagg cggtggtagc caggcgggtg
      961 gatcagctca cacaggttca caaaactcag ctcaatcaca agcttctaag caattagcta
     1021 ctgaaaaaga atcagctaaa aatgccattg aaaaagcagc caaggacaag caggatgaaa
     1081 tcaaaggcgc accgctttct gataaagaaa aagcagaact tttagcaaga gtggaagcag
     1141 aaaaacaagc agctctcaaa gagattgaaa atgcgaaaac tatggaagat gtgaaggaag
     1201 cagaaacgat tggagtgcaa gccattgtca tggttacagt tcctaagaga ccagtggctc
     1261 ctaatgctgc tcctaagaca acaagtgcac cgcaagcaac tgcaggaaca atgcaagatg
     1321 ttacctacca gtcacctgct ggcaaac
//
LOCUS       DAVBCR010000139         1327 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBCR010000139 DAVBCR010000000
VERSION     DAVBCR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.139"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            236..676
                     /locus_tag="VQ508_002035"
     CDS             236..676
                     /locus_tag="VQ508_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2490973.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            673..1131
                     /locus_tag="VQ508_002036"
     CDS             673..1131
                     /locus_tag="VQ508_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2490974.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          421 a          288 c          249 g          369 t
ORIGIN      
        1 ccttgccaga gggagtaaaa tagtggtata ctcaataaaa atcaaagagc aaactagaaa
       61 gctaggcgca gacagctcaa aacactgttt tgaggttgta gataaaactg acaaagtcaa
      121 caactatcct acggcaagac gacgctgacg tggtttgaag agatttttga agagtattaa
      181 taaaggtgta caagaagtga gattaatttt gtatgaaaaa caaggagaga aatttatgaa
      241 agtagaatta cagattagtg agacttacga ggaggaaaag ctgattgtcc aagcacctca
      301 gccgacagat aaagttcaga aagtcatcga gttcgcagaa aatctggacc aaagagaaaa
      361 aatcaaaggg aaaatcgatg atcaggtcta tctagttgag attggcaaga ttcagcgctt
      421 ctatatcgag aatcggaagg ttctagcaga aaccgcgagt cagacctaca gcattgattt
      481 gcgactctat caggttctta aactcttgcc aaccaatttt atccaaattt cccaatcaga
      541 aatcatcaat atcgattcca tctctcacct caagctcacg ccaaacggtc tggtagaaat
      601 tttcttgaaa aacgaaagct tcacctactc ttcacgccgt tatctaaaaa ccatcaagga
      661 gaaattagaa ctatgaaaaa acaagtattt cacgatgcag ctgccggtgt tcttatcggc
      721 ctcatcctct ctatcctctt ttcactcatt tatgcaccaa atacctacgc accactaaat
      781 ccctactctc tcatcggcca agtgatggat cagcatcagg ttcacggtgc cttggtcttg
      841 ctctactgca cacttatctg ggcagccatt ggtatgctct tcaactttgg caatcgctta
      901 ttcagccgtg actggagcat gcttcgtgcc actctgactc atttcttcct tatgctggct
      961 ggctttgtcc cactagcaac tcttgctggt tggttccctt tccactggat tttctacctc
     1021 cagctcatta tcgagtttgc gattgtctat ctcatcatct gggctattct ctataaaaga
     1081 gaggctaaaa aagtagatca catcaatcaa ctcttggagc atagaaaata ggaacctcat
     1141 aataagcaaa tccgagaaag aaacttctcg gtttttcttt atcctgctgc accttactat
     1201 agtgaaatga aataagaaca ggataaatcg atcaggacag tcaaatcgat ttctaacaat
     1261 gttttagaag cagaggtgta ctattctagt ttcaatcctc tatatcataa cagttatttc
     1321 agatctt
//
LOCUS       DAVBCR010000140         1302 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBCR010000140 DAVBCR010000000
VERSION     DAVBCR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.140"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            168..854
                     /locus_tag="VQ508_002037"
     CDS             168..854
                     /locus_tag="VQ508_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2490975.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKTAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..>1302
                     /locus_tag="VQ508_002038"
     CDS             855..>1302
                     /locus_tag="VQ508_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2490976.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGII"
BASE COUNT          433 a          174 c          244 g          451 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa aaccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgcta tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gattttttat
     1141 tctttttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat ta
//
LOCUS       DAVBCR010000141         1190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBCR010000141 DAVBCR010000000
VERSION     DAVBCR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.141"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQ508_002039"
     CDS             239..>1190
                     /locus_tag="VQ508_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2490977.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBCR010000142         1159 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBCR010000142 DAVBCR010000000
VERSION     DAVBCR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.142"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(58..381)
                     /locus_tag="VQ508_002040"
     CDS             complement(58..381)
                     /locus_tag="VQ508_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2490978.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            complement(371..1027)
                     /locus_tag="VQ508_002041"
     CDS             complement(371..1027)
                     /locus_tag="VQ508_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2490979.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSSFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
BASE COUNT          399 a          282 c          228 g          250 t
ORIGIN      
        1 atcatttaca ccattatatg atatatatat ctgtgatagg ttttctttat ggctgattta
       61 aaagaccaaa cgcaagacgg caatcaagac tactccaaag agaactgttc cgactagatt
      121 gcggtagcga aaggctaccc aagctgttgg aaagacggct aagaagtcta gccatttaat
      181 ttgaggaagg ctcccaacct tacctgtcac tacgcttgaa agaatcaagg caaagataat
      241 ggaaacgggc aagaacttca aaaaacgctc aacgattgca ggcaagccct tatacttgac
      301 taagatgaag ggaatcatac ggagaatcca agtcaccaag tcagagaaaa taactgctaa
      361 taaaagatac ttactgacca tctaaaacca cccccatgct acaaccaagt agcgtcgcaa
      421 acagaacagc tagtgactga gacaccactg tcaagagcaa aaaggaggac accgcaacaa
      481 ctgctaggat aatgagcaga ttgcggacag gaatccgtct ttgcataatc tgaaattgcg
      541 aagcaaaaat accaataaac atcccaacca gggcaaaatc caagccaaag atttctggat
      601 ttggtagcag gccacccaga gccgttccga ctactgtccc cacaaaccaa gccacatagc
      661 tgttaagatt gtttccgtgc atccacatag gatttacctt gtctgtatgg gccaattcac
      721 ccatcaaaac gccataggtc tcatctgtca agatactaga cataccgata ttgtaccaaa
      781 gactggtatg acggaaataa gtcgatgcgt gtaaactcaa caaaaagaga cgcaagttga
      841 ttagaaaaac cgtcatagca atagctgcca caggagcttg aaccacaatc agtgccaaca
      901 tggcaaactg ggcactccca gcataaacaa agagactcat caagcccatc tcaacaggtg
      961 tcacataggg cgcaccgata gtcccacagg ccaggccgat actgacatag ccaagagccg
     1021 ttggcatggc tgcctgcgcc ccctcctaaa atcctttttc tttcatcttt ctcctcatat
     1081 tgtcttaata atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggttgct
     1141 caaaacattg ttttgaggt
//
LOCUS       DAVBCR010000143         1140 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBCR010000143 DAVBCR010000000
VERSION     DAVBCR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.143"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..107)
                     /locus_tag="VQ508_002042"
     CDS             complement(<1..107)
                     /locus_tag="VQ508_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2490980.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASL"
     gene            complement(565..963)
                     /locus_tag="VQ508_002043"
     CDS             complement(565..963)
                     /locus_tag="VQ508_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2490981.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLQIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          347 a          221 c          168 g          404 t
ORIGIN      
        1 agagaggcaa tttctctatt tgattttcct tcttttttcc atcgttcgat taagcgacgg
       61 ctatcgattg tcaaatgttt ggcttttgta gtataattgt cttgcatctc tgtgcctttc
      121 ttgtgtttgt ggttgaacaa caagtataac acagaggtgt tttcttatgc ctacaagagt
      181 tttcattatt tccattttct ttcggatttc actctattct gaaaaacttg tgtatattta
      241 ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc ctagagggga
      301 atctcggatt aatgtcaatt taaaaagata gagagttttt agtttaaata aattgtttta
      361 aaatatcaac aacatcactt cttttcttaa cctgataagc cttgattcct aattttggag
      421 ctacgattat attgtcctca atatcgtcta gaaagacaca atttctaggt tataactggt
      481 atttatcgat agttactcat atacatcagt ccacctccat acttatgtgc gagcctctct
      541 ttgtattata cctccatact caccttacag attcttttgg taataatatc tttgcctaat
      601 gtagagatag tcttgcaaag aaaaaacttc cttgtagcca tgtttctgat aaaagtccgg
      661 tgcctggaac tgataagtat tgacaaaggc aaaacaacaa tttcgattct tagcttcact
      721 ttctgcctgt tgcaatagtt ttgaaccgat tccttgccct cgcagttcct cttttacaaa
      781 caaatactcg atttgtagcc aatttccaaa agtctctgct atcaaacccg caaggagatt
      841 gcccttttca tcttcgacat aaagattaag tggctcactt tcagcctctt ccctttttga
      901 acggttataa acacgaatca gattccctat ttcttgcgat ttatgtgatt ccttattttc
      961 caatctaaag tataatgaaa tgaaataaaa catgcgcaaa tcgattaagg aatttaatct
     1021 aatttctaat aatgtcttag aaatcaaagt ttactatttt aacttcaatg cactatacat
     1081 ctaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggtt gctcaaaaca
//
LOCUS       DAVBCR010000144         1084 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBCR010000144 DAVBCR010000000
VERSION     DAVBCR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.144"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(88..237)
                     /gene="blpO"
                     /locus_tag="VQ508_002044"
     CDS             complement(88..237)
                     /gene="blpO"
                     /locus_tag="VQ508_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2490982.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(481..684)
                     /gene="blpN"
                     /locus_tag="VQ508_002045"
     CDS             complement(481..684)
                     /gene="blpN"
                     /locus_tag="VQ508_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2490983.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(700..954)
                     /gene="blpM"
                     /locus_tag="VQ508_002046"
     CDS             complement(700..954)
                     /gene="blpM"
                     /locus_tag="VQ508_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2490984.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
BASE COUNT          351 a          261 c          132 g          340 t
ORIGIN      
        1 ttgctgcata ggctacacct ccaagtatag ctccacctgc agcaccagtt acacctattc
       61 ctatagcaaa tggtcccaat agaaatgtca aaccgttgtt gcacacccat caattgcgcc
      121 atatgcaacc cctgctgcac aactaatttt tcttccccaa tcaatatctc caccttcaac
      181 gcaagcaagc atttcattat ccataactgc aaattgtgac atcatttttg tatccatata
      241 gtatatcact tttcagttac ggaacaagtt taatataaaa attatcaaaa aaacataggc
      301 aataaagaga aaaattaatt tatcatagat tagaaataat atgacaaaac aattcaatga
      361 tgttaattca atagtctttt gttttttatc ggagatactt atggatagat aaataagata
      421 ggtttgaaaa gcgaagagaa taataaagaa tatagccttc ataaaattta gctttcattt
      481 ttatgatgta gcggtatagg ctaaatatcc acaaaccact gctcctccaa ttcctcctat
      541 tgcagcgccc catggtccta gaagtctccc atatttcact ccacccgctg cacaacctaa
      601 agcagcaact acagctgctc ctccggaatt acctccataa acctcactca gcattgtttc
      661 atttatatta caataagtat tcatacaagt ctccttttat taaaatccac ccgttgcccc
      721 tgttactcct gcccaaagat ccacaccaaa tttagctcct atgtatccac atgctcccat
      781 aaatggtgct ccaacaccac tcgcagcaca aatagctgtc cctagccccc agccaccaaa
      841 agcagcacca ccaccttcca agacattagt ttgccaatta ttcttgcctc cttcaatact
      901 agataacata gttatatcca tttcatgaaa ttgttccata atttttgtat ccatgacaaa
      961 tactcttttt tatttttaat ttttgtcttg ttgtaacttt gacaagttta gtatatcatc
     1021 gttttttaaa atttttcatc cagatcttga atagtcatcg aaacgtcttg aattgcaaaa
     1081 atta
//
LOCUS       DAVBCR010000145         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBCR010000145 DAVBCR010000000
VERSION     DAVBCR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.145"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ508_002047"
     CDS             <1..555
                     /locus_tag="VQ508_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2490985.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ508_002048"
     CDS             complement(503..1048)
                     /locus_tag="VQ508_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2490986.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCR010000146         1035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBCR010000146 DAVBCR010000000
VERSION     DAVBCR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.146"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ508_002049"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ508_002049"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2490987.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBCR010000147         1032 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBCR010000147 DAVBCR010000000
VERSION     DAVBCR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.147"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQ508_002050"
     CDS             complement(741..1001)
                     /locus_tag="VQ508_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490988.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          342 a          196 c          166 g          328 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga gcagcatttc ctagccaata ggacgctcca aatcacctat tctaaatcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtgtt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaatgtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa tt
//
LOCUS       DAVBCR010000148          973 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBCR010000148 DAVBCR010000000
VERSION     DAVBCR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.148"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            25..798
                     /locus_tag="VQ508_002051"
     CDS             25..798
                     /locus_tag="VQ508_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2490989.1"
                     /translation="MLAACSGGAKKEGQAASKKEIIVATNSSPKPFNYEENGELTGYE
                     IEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAVNNISYTKERAEKYLYAAPT
                     VKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQLEDYNKQHSDNPTILNYTKA
                     NLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVIDLPSDQQPYVYPLLAKGQD
                     ELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK"
     gene            839..916
                     /locus_tag="VQ508_002052"
                     /pseudo
     CDS             839..916
                     /locus_tag="VQ508_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
BASE COUNT          324 a          195 c          192 g          262 t
ORIGIN      
        1 gccctagggc ttgttgctgc aggtgtgcta gcagcttgct ccggtggtgc taagaaagaa
       61 ggacaagcag ctagcaagaa agaaattatt gttgcaacta attcttcacc aaaaccattc
      121 aactatgaag aaaatggcga attgactggt tacgagattg aagtcgttcg cgctatcttt
      181 aaagactctg ataagtatga tgtcaagttt gagaagacag agtggtcagg tgtctttgcg
      241 ggtcttgacg ctgaccgtta ccaaatggct gttaacaaca tcagctacac taaagaacgt
      301 gcggagaaat acctttatgc agccccaact gttaaagacc ctaacgttct tgtagtcaag
      361 aaagacgatt ctagcatcaa atcgcttgat gatgttggtg gaaaatcaac agaagttgtt
      421 caaggaacaa catctgctaa acagttagaa gactacaaca aacaacactc agataatcca
      481 actatcctta actatactaa agcaaactta caacaaatca tgggccactt gagcgatggt
      541 cagtttgact acaagatttc tgataaaatc actgttgaaa cagtcatcaa gaaccaaggt
      601 ttggacaact tgaaagtcat cgatcttcca agcgaccaac aaccttacgt ttacccactt
      661 cttgctaaag gtcaagatga gttgaaaaca tttgtagaca aacgtatcca agaactctac
      721 aaagacggaa ctcttgaaaa attgtctaaa caattcttcg gagacactta cctaccggca
      781 gaagctgata ttaaataatt tcttgaaatc atccattctg agtaagatgg gtgatttttc
      841 agttcgctac cgcaatctag tcggaacagt tctctttgga gtggtcttga ttgccatctt
      901 gcgtttggtc ttttaaatta cccataaaaa aaacttatca cagagataga tatcatataa
      961 tggcgtaaat gct
//
LOCUS       DAVBCR010000149          922 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBCR010000149 DAVBCR010000000
VERSION     DAVBCR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.149"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ508_002053"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ508_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          139 c          172 g          275 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 actagcatag atgagcgctt gc
//
LOCUS       DAVBCR010000150          860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBCR010000150 DAVBCR010000000
VERSION     DAVBCR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.150"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            101..265
                     /locus_tag="VQ508_002054"
     CDS             101..265
                     /locus_tag="VQ508_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490990.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            262..666
                     /locus_tag="VQ508_002055"
     CDS             262..666
                     /locus_tag="VQ508_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2490991.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          257 a          132 c          151 g          320 t
ORIGIN      
        1 atttatgatt tgagtgatcc aaggtcaaaa taagtgctat actagcagtg taaatgttct
       61 tgctcaacta agataaatcg cacatttagg aggaaatctt atgaagaaaa aaatactgat
      121 tattttcgtt ctgtatctaa tcatgtccat ctttctatat ccgcttaggg agagtgcttg
      181 gtatcagcta ttttatacca tagcctatgt gattgcagtt atgatctatt ttgctatcaa
      241 taaaaagaaa ggagaaaaga aatgaaaact ttttttgcta aaaaacggaa catcttcctt
      301 gcgagattgt tcttaggtca gttgcccttg cttgtttcta cttatctatt tctatctcgt
      361 cagtttttaa atttttccgt agttttccaa tttcttttag tagttattaa cttggcttct
      421 attttggtta ctgtttatct cactagggaa atgaggataa gagagtttga agatgatgat
      481 ttggttagtc ctagaaccaa tcaacttatg ttcatcggct tgacaggttt tatgtctatt
      541 atttgtttgt atagaggtat cacagcagga gaatcctacc aacaaataat cgcttatatt
      601 ggcgctgttc tctgcttaat catcatgctt ctgctcatgt ggggcttgaa gtattataaa
      661 aagtagagct taatgaagta taaaagaaaa gggtaagtgt ttttgcaata cttacccttt
      721 tataatagat tccctgcgaa acaaaatatg gtatagtata gtatattgaa actagaatag
      781 tacgcctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttatcc
      841 tgttcttatt tcattttact
//
LOCUS       DAVBCR010000151          834 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBCR010000151 DAVBCR010000000
VERSION     DAVBCR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.151"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..>834)
                     /locus_tag="VQ508_002056"
     rRNA            complement(<1..>834)
                     /locus_tag="VQ508_002056"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          178 a          267 c          176 g          213 t
ORIGIN      
        1 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
       61 gcaccacctg tcacctctgt cccgaaggaa aactctatct ctagagcggt cagagggatg
      121 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      181 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      241 cttaatgcgt tagctacggc actaaacccc ggaaagggtc taacacctag cactcatcgt
      301 ttacagcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      361 cgtcagttac aagccagaga gccgctttcg ccaccggtgt tcctccatat atctacgcat
      421 ttcaccgcta cacatggaat tccactctcc cctcttgcac tcaagttaaa cagtttccaa
      481 agcctactat ggttaagcca cagcctttaa cttcagactt atctaaccgc ctgcgctcgc
      541 tttacgccca ataaatccgg acaacgctcg ggacctacgt attaccgcgg ctgctggcac
      601 gtagttagcc gtccctttct ggtaagatac cgtcacagtg tgaactttcc actctcacac
      661 tcgttcttct cttacaacag agctttacga tccgaaaacc ttcttcactc acgcggcgtt
      721 gctcggtcag acttccgtcc attgccgaag attccctact gctgcctccc gtaggagtct
      781 gggccgtgtc tcagtcccag tgtggccgat caccctctca ggtcggctat gtat
//
LOCUS       DAVBCR010000152          832 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBCR010000152 DAVBCR010000000
VERSION     DAVBCR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.152"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            377..526
                     /locus_tag="VQ508_002057"
     CDS             377..526
                     /locus_tag="VQ508_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET2490992.1"
                     /translation="MGDQPVQDTETSSALILSHYLDEQDLSEKLKSDLQWFELENKLL
                     NLWEY"
     gene            514..804
                     /locus_tag="VQ508_002058"
     CDS             514..804
                     /locus_tag="VQ508_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2490993.1"
                     /translation="MGVLGYYEAFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT          313 a          108 c          160 g          251 t
ORIGIN      
        1 ttatctacta cctcaaaacg gtgttttgag caacctgtgg ctagtttcct agtttgctct
       61 ttgattttca ttgagtacaa agagcttggc aacaggctct ttttgtattg cgtttattaa
      121 gaaaaacaaa aaacatcgat atacatttaa ctaaaaatta ttgtatgtta tctcccttaa
      181 ttaggaatga taagggaata actagaaaga tttgtgaata caaactattt ctgatataca
      241 aaatatacag taataatgaa tgatgggaga tgggatgaaa gaatttcaat ttgagagaaa
      301 gccgcgtttt tctttgaggc catatgcaat aggagcttgt taggtcttgc taggaacgag
      361 tttatttttt gctggtatgg gtgatcagcc tgtacaggat acagaaacga gttcagcact
      421 aattttaagt cattatttgg atgagcagga tttatctgaa aagctgaaat ctgatttgca
      481 atggtttgaa ttagaaaaca agcttttgaa cttatgggag tattaggtta ctatgaagca
      541 tttgttcctt atgtttcaaa tcaatacaaa aaccaagctg aagaagaagg caaaccgcta
      601 tctgataaat atattttcga aaaaatatta ggaaaaacat atgcagcctt caaaaaagat
      661 caaattaatg agcgtgtcga gaaattaggt aagttaaaac ctattacaat aaattacaac
      721 ggaaaatcag aagtaattga tagtaaagaa aaattacaag agcttatgaa taaagccgtt
      781 aaagacgaag tggctcaaat atagtaaaat gaaataagaa caggacaaat cg
//
LOCUS       DAVBCR010000153          810 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBCR010000153 DAVBCR010000000
VERSION     DAVBCR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.153"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(144..731)
                     /locus_tag="VQ508_002059"
     CDS             complement(144..731)
                     /locus_tag="VQ508_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2490994.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(734..>810)
                     /locus_tag="VQ508_002060"
     CDS             complement(734..>810)
                     /locus_tag="VQ508_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2490995.1"
                     /translation="MPQSPWVERIVQVLDLDYEDLFRR"
BASE COUNT          298 a          167 c          111 g          234 t
ORIGIN      
        1 tctctccacc agtaaaatgc aagcaattat acaatgttgt cacatagaaa ataatgtttc
       61 cgtaactttt caaagtaact tccatctctc tcccaaaact ggaagttagt tttagaagtt
      121 acctaaaaat caggtcacct attttaaaaa agcagcaaac tataaactag taggttccac
      181 accaaatgta gtcccatact gccccataag tcagatttat agcgcaccat acctaaaaac
      241 atcccaagtg aaacatacaa acaccaagct agaatggttc ctgtatgatg tgctaaggca
      301 aataaaacac ttgtcaaagc aactctgata tctaattttc tgaccaaatt ccataaaatt
      361 tctcgataca gaaattcttc aaccatactc gcattgatta agaacaataa aaatgaaaac
      421 caaggaattt gatgttgaag gccaattaag tttgcttgat tcgtgcttcc ttgagcatga
      481 atcagactaa aacatagact tataatcagt aggctaacaa attcaacacc aagccatttc
      541 atcctagatt tcatattgac cttatgcgct tgtttgcgtt ggccatacat ccataaaaaa
      601 gaaatgagtg acgaaccata gagaatctgt agtatagtta actcaccgat acaaagaaat
      661 ttcaataagt atagagatac caataggaca tttacttgtt ggaatatata aactggaatt
      721 attcttttca tagttacctc cgaaataaat cttcataatc taaatctaat acctgcacaa
      781 tcctttctac ccatggactt tgaggcattc
//
LOCUS       DAVBCR010000154          692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBCR010000154 DAVBCR010000000
VERSION     DAVBCR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.154"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ508_002061"
     tRNA            complement(74..147)
                     /locus_tag="VQ508_002061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ508_002062"
     tRNA            complement(157..242)
                     /locus_tag="VQ508_002062"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ508_002063"
     tRNA            complement(250..321)
                     /locus_tag="VQ508_002063"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ508_002064"
     tRNA            complement(344..416)
                     /locus_tag="VQ508_002064"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ508_002065"
     tRNA            complement(435..516)
                     /locus_tag="VQ508_002065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ508_002066"
     tRNA            complement(522..594)
                     /locus_tag="VQ508_002066"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ508_002067"
     tRNA            complement(619..691)
                     /locus_tag="VQ508_002067"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVBCR010000155          671 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBCR010000155 DAVBCR010000000
VERSION     DAVBCR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.155"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>671
                     /locus_tag="VQ508_002068"
     CDS             <1..>671
                     /locus_tag="VQ508_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2490996.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKR
                     IARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAV
                     RKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDRE
                     FYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIR
                     HPERLG"
BASE COUNT          210 a          148 c          137 g          176 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aaccgccaga
       61 ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca aatgtctgaa
      121 ttggaaaaac gaattgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaagac cagaacaacc aagtccacaa tcgactccgg aacctagtcc aagtctgcaa
      241 cctgcaccaa atcctcaacc agctccaagc aatccaattg atgagaaatt ggtcaaagaa
      301 gctgttcgaa aagtaggcga tggttatgtc tttgaggaga atggagtttc tcgttatatc
      361 ccagccaagg atctttcagc agaaacagca gcaggcattg atagcaaact ggccaagcag
      421 gaaagtttat cgcataagct aggagctaag aaaactgacc tcccatctag tgatagagaa
      481 ttttacaata agacttatga cttactagca agaattcacc aagatttact tgataataaa
      541 ggtcgacaag ttgattttga ggctttggat aacctgttgg aacgactcaa ggatgtctca
      601 agtgataaag tcaagttagt ggatgatatt cttgccttct tagctccgat tcgtcatcca
      661 gaacgtttag g
//
LOCUS       DAVBCR010000156          638 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBCR010000156 DAVBCR010000000
VERSION     DAVBCR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.156"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            11..>638
                     /locus_tag="VQ508_002069"
     CDS             11..>638
                     /locus_tag="VQ508_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490997.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIG"
BASE COUNT          201 a          142 c          116 g          179 t
ORIGIN      
        1 aggtactccc atgctttatg tgggcattga tatcgctaaa aataaacacg atgttacagc
       61 cttgaatgtt ccaggaaaaa ctgttcttaa accactcact ttttcaaata ataaagctgg
      121 ttttgaactc ttagatctgt ctcttcgaca gctcaaccaa gactgtctca tcgctcttaa
      181 acttctttct gaccccaatc gtgaacaatt tcaacacgat aatcggcaag tagacctaaa
      241 aatactggct agacatattc atcgtctcaa gaaaaaacag tctgattgga aagtacaata
      301 cactcgttgt cttgatatca tctttcctga gttggataaa atcgttggaa agcattcaga
      361 atatacctac caactcttga cgcgctaccc taatcctcag aaaaggattg aggcaggatt
      421 tgataagctg atagaaatta agcgattgac cgcttctaaa attcaggata tcctctcagt
      481 cgcacctcgt tctatcggaa caacatctcc tgctcgtgaa ttcgaaatca tcgaaatcat
      541 caaacattac aagaggctca ttgacaaggc ggaaacatgt gtcaatgact tgatggctga
      601 gttcaactca gtcatcacga cggttactgg gattgggg
//
LOCUS       DAVBCR010000157          628 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBCR010000157 DAVBCR010000000
VERSION     DAVBCR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.157"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            519..>628
                     /locus_tag="VQ508_002070"
     CDS             519..>628
                     /locus_tag="VQ508_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2490998.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          222 a           95 c          109 g          202 t
ORIGIN      
        1 ttcctaatcg atttgttcat atcttatttc aatccattat aaatagcgag aaatatctat
       61 cctatcttct agaatgtctt ccaaacgagg aaactctcgt aaacaaagag gttttagagg
      121 cctatttacc atggactaaa gttgtacaag aaaagtgcaa ataagaaatc tccagattag
      181 gaactatccg tgagttcact aatctggaga tttttcaata gacttcgtta ttgggcggtt
      241 acgatatgat cactgacttc gtcagtctta tctacaacct caaaactgtg ttttgagcaa
      301 cctgcggcta gcttcctagt ttgctctttg attttcattg agtattagaa cagaaaaaat
      361 acttggagta tttgtttgtg tgtttatttt tatataacaa actataaaca aaacaaaaat
      421 ataaaaaaag agacaaaaaa gaacagaaag caattgacaa cggtttcata tagtgttata
      481 cttatggcat aaagttaatg gaaagaaggg aaaaacttat ggtattagat tatttctttg
      541 acaaaaacct tgtgttttgc ttagaagcgg ataatcaaga acaactcttt gatcaggttg
      601 caactttatt ggaagaacga gaaattgt
//
LOCUS       DAVBCR010000158          623 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBCR010000158 DAVBCR010000000
VERSION     DAVBCR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.158"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
BASE COUNT          194 a          120 c          158 g          151 t
ORIGIN      
        1 ttttaagtac gacgggcatg tcgtgcatct gaggtgtaag tcctccgtgg gcacctgcta
       61 ccggtgaacc caatagcgat tcccaagcct gactatcgtg aggtagcgga ttaaaacggt
      121 ctggggatag accgttttaa gtctgacgca ggaaataaga atcgtcagag gaagggatag
      181 cgaaatcgag gctctacgaa caggaacgtg atagtaaggc gtatacagcg gataaggggg
      241 cttaaaactc taaagtccaa aaaggtagtc gtaacctata tgcgtaaatc acgagagtaa
      301 ttgaattcgg actaaggtct atgtgaaaaa gataaatctt cctagagtct aaagactctg
      361 cgtcagattt cctattttca ttgtaacctt ttaacgtcct catatcttgt ataaacgagg
      421 aaagatgtac ggcttatccc gtgaggtctc atgagcgccg aaagcgtagt aacaacgaat
      481 catgagaagt cagccgagcc catagtagtg aggaaacttc tgtaatggaa gtggagcgaa
      541 ggggtaaaca actaatcgaa gtaatcctac ttcacttgtg tctgtaaaac gaacggtctg
      601 atagaactgg actctgtcac gta
//
LOCUS       DAVBCR010000159          615 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBCR010000159 DAVBCR010000000
VERSION     DAVBCR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.159"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..198
                     /locus_tag="VQ508_002071"
     CDS             <1..198
                     /locus_tag="VQ508_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2490999.1"
                     /translation="SKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTYRNH
                     RKRFGLRMNLIAGIINHELGF"
     gene            401..>615
                     /locus_tag="VQ508_002072"
     CDS             401..>615
                     /locus_tag="VQ508_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2491000.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          226 a           84 c          139 g          166 t
ORIGIN      
        1 agcaaactca agccgctaac agttgaagat aaagcctgta atcatgcgct atctaaggag
       61 agaagcaagg ttgagaatat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc
      121 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat
      181 catgaactag gattctagtt ttgcaggaag tctaatagta aaaaagtgat tagaaaacat
      241 cttttttaaa aatagagatg attttgaaac aaaaaagcta attcaagacg tttcgatgcc
      301 aattcaagat ttggatgaaa aaaattaata gatactgtta tactaaactt gtcaagtttg
      361 taacaagaca aatattaaaa ataaaaaaga ggtattcgtt atgaatacaa aaaagatgtc
      421 acaatttgaa attatggata ctgagatgct tgcttgcgtt gaaggtggcg gatgcaattg
      481 gggagatttt gccaaagcag gtgttggagg aggagcagta cgaggtcttc agctaggaat
      541 taaaacaaga acatggcaag gtgcagcaac tggtgctgcg ggaggagcta tacttggagg
      601 tgtggcctat gcagc
//
LOCUS       DAVBCR010000160          610 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBCR010000160 DAVBCR010000000
VERSION     DAVBCR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.160"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(188..>610)
                     /locus_tag="VQ508_002073"
     CDS             complement(188..>610)
                     /locus_tag="VQ508_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2491001.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          155 a          121 c           92 g          242 t
ORIGIN      
        1 atgaagtttt ttctcaactt cttttacttt attaaatagt tttctaaacc ggttatttaa
       61 ccatttaatt aaccagtaaa tagtttacta tttaaaatta cttttgtcaa gtttctgtta
      121 cgtttttcta aaaaagagaa gtaacacgaa aggttacttc tccataaaat atgctccatt
      181 cattttacta ctgtatagga gtcggttgac tttcttttaa caaagccaag agactatcta
      241 cttctgctga aatagtgtta ttatctttcg ttccgagaag aagactactt tttagaccag
      301 tcaatgtctc cacagcattt tgtctaatac tagaatctgt tactttttct agcaaggcct
      361 ccgcctctgc tatcttagcg ttaataacag aatgctctac ttgaggaatt tcagcctcat
      421 ctgtagtatc ttcagcttct tcctctgtct cttccgtatc agtgcttggt ttataaagat
      481 tatctgctga aggatttaaa ccagcgtgat tctctttttc atcagattca gggtgagttg
      541 gctcacttac ttcatcatgt tccttatctt cctcaggttt ttctgtctga ggtttctcct
      601 cgtttggttt
//
LOCUS       DAVBCR010000161          572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBCR010000161 DAVBCR010000000
VERSION     DAVBCR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.161"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            212..427
                     /locus_tag="VQ508_002074"
     CDS             212..427
                     /locus_tag="VQ508_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2491002.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYIE
                     NVYLDSLSVKQKFKFIFDFGDEWRFEC"
BASE COUNT          190 a           83 c          127 g          172 t
ORIGIN      
        1 aaaacaagaa caccttgctt ggcgcagggt gtttcgcgtg aaacagaaga attatctggt
       61 ttcaaatgct acagttaaaa ttagtaaaac aagaaataga agctgaaaag ccagcatctg
      121 tagaagcttg gatcatttcc gtcaaattaa aaaaggttgc taccgacata tatagattcc
      181 aaaaacaaaa acgttagcgg aactagcaga tgtgatttta tggagtttcg attttgcaaa
      241 tgatcatgct catgcatttt tcatggataa tgttgagtgg agtcatgcag attcttactt
      301 tcttagcttt gttagtgacg atgttgaaga acgttacata gaaaatgtct atctggatag
      361 cctaagtgtc aaacaaaaat ttaagtttat tttcgacttc ggtgatgaat ggcgttttga
      421 atgctaagtg ctgagagaaa tcgagacaga ggacgaagaa gcttatctcg tacgttcggt
      481 tggaacgttg ccagaacaat atccagatta tgatggtttt gactatgaag aatggtaaaa
      541 ttgaaatcag tctgtgtagg cttagtattt ca
//
LOCUS       DAVBCR010000162          534 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBCR010000162 DAVBCR010000000
VERSION     DAVBCR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.162"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
BASE COUNT          172 a           89 c          139 g          134 t
ORIGIN      
        1 ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct ctacgaacag
       61 gaacgtgata gtaaggcgta tatagcggat aaggaggctt caaactctaa agtccaaaaa
      121 ggtagtcgta acctatatgt gtaaatcacg agagtaattg aattcggact aaggtttgtg
      181 tgaaaaagat aaatctttct agagtctaaa gactctgcgt cagatttcct attttcactg
      241 taacctttta acgtcctcat atcttgtata aacgaggaaa gatgtacgac ttatcccgtg
      301 aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat gagaagtcag ccgagcccat
      361 agtagtgagg aaacttccgt aatggaagtg gagcgaaggg gtgaatactc aaacagtctg
      421 gggagagact gtttgaggtc tgtcgctaga aagagaaaac gacagatcga agtaatccta
      481 cttcacttgt gtctgtaaaa tgagtggtct gatagaactg gactttgaca cgta
//
LOCUS       DAVBCR010000163          530 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBCR010000163 DAVBCR010000000
VERSION     DAVBCR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.163"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..530)
                     /locus_tag="VQ508_002075"
                     /pseudo
     CDS             complement(1..530)
                     /locus_tag="VQ508_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          170 a           95 c          115 g          150 t
ORIGIN      
        1 ttaaagacag tctgaaaaat aggatgaacc tgcttcagat tgtcctcaat aagtccgaaa
       61 aatttctctg gttccttatt ctggaagtga aaaagcaaga gctgatagag ctgatagtgg
      121 tgtttcaagt cttccgaata gctcaaaagc ttgtctaaaa tctctttatt ggttaaatgc
      181 atacgaaaag taggacgata aaaacgctta tcactcagtt tacggctatc ctgttgtatg
      241 agcttccagt agcgcttgat agccttgtat tcatgggatt ttcgatgaaa ctgattcatg
      301 atttaaacac gcacacgact catagcacgg ctaagatgtt gcacaatgtg aaagcgatca
      361 agaacgattt tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc ctgagcctag
      421 aaatttgaaa gcgaagctgt ctagctaagt catagtaagg gctaaacata tccatagtaa
      481 taattttgac gcgacatcgg acaactctat cgtagcgaag aaagtgattt
//
LOCUS       DAVBCR010000164          526 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBCR010000164 DAVBCR010000000
VERSION     DAVBCR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.164"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(238..325)
                     /locus_tag="VQ508_002076"
     tRNA            complement(238..325)
                     /locus_tag="VQ508_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ser,seq:gct)
     gene            complement(332..405)
                     /locus_tag="VQ508_002077"
     tRNA            complement(332..405)
                     /locus_tag="VQ508_002077"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Ile,seq:gat)
     gene            complement(441..511)
                     /locus_tag="VQ508_002078"
     tRNA            complement(441..511)
                     /locus_tag="VQ508_002078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Gly,seq:tcc)
BASE COUNT          176 a          119 c           92 g          139 t
ORIGIN      
        1 gcaatgcctt tggttttcga aactttgaaa actttaaaaa acggattttt atcgctttga
       61 acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
      121 tacagttgac aaagagccat aaaaataact tcagattagt aattcaatta agttttacgg
      181 atctgaagct ttattgtctc taaaaataaa aaagagagtt acaaactctc attaaaacgg
      241 agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag caaaccgtcc
      301 tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga accaccgacc
      361 tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt aaaaaacttg
      421 gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa
      481 ggctgtagtt ttaccactaa actacacccg cataaatact atcaat
//
LOCUS       DAVBCR010000165          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBCR010000165 DAVBCR010000000
VERSION     DAVBCR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.165"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            152..460
                     /locus_tag="VQ508_002079"
                     /pseudo
     CDS             152..460
                     /locus_tag="VQ508_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT          204 a           64 c           97 g          148 t
ORIGIN      
        1 tatcgcttac caatatttgc taaactttca gaaaaaaagt tggagtatat gatgctaggt
       61 gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga taacttattt
      121 gaaaaaggga aaaatgagat aaaagattca agtagtagat ttagcaatag gacatataaa
      181 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
      241 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
      301 ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa atgcagacaa
      361 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
      421 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
      481 ctgtattttg gaaaaattac ttaacctttt aat
//
LOCUS       DAVBCR010000166          500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBCR010000166 DAVBCR010000000
VERSION     DAVBCR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.166"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(4..381)
                     /locus_tag="VQ508_002080"
     CDS             complement(4..381)
                     /locus_tag="VQ508_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2491003.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
BASE COUNT          143 a           94 c           97 g          166 t
ORIGIN      
        1 atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac
       61 aattgcgata gcgtatacta gtgcgatttg tttgtagctc tggtgggtat caccttcacg
      121 ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc ataggcgatt
      181 gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa
      241 ctccgccaag acgtctatac tagtatagaa atcttgcgct gagtagggca tggaaggcaa
      301 gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc
      361 gcggataaat tgacaaatca ttagttgggc tgatacctgg atgtctgaga atcctccttt
      421 tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct
      481 atttattcgg aaaagaaagc
//
LOCUS       DAVBCR010000167          482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBCR010000167 DAVBCR010000000
VERSION     DAVBCR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.167"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            30..>482
                     /locus_tag="VQ508_002081"
     CDS             30..>482
                     /locus_tag="VQ508_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2491004.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          178 a           62 c          116 g          126 t
ORIGIN      
        1 gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa gaagtccaag
       61 ggactgtgta taagtgtaga aatgaatatt accttcattt atgggaattg tcggattggg
      121 accaagaagg catgctctgc ttacatgaat tgattagtag agaagaagga ctggtagacg
      181 atattccacg tttaaggaaa tatttcaaaa ccaagtttcg aaatcgaatt ttagactata
      241 tccgtaagca ggaaagtcag aagcgtagat acgataaaga accctatgaa gaagtgggtg
      301 agatcagtca tcgtataagt gaggggggtc tctggctaga tgattattat ctctttcatg
      361 aaacactaag agattataga aacaaacaaa gtaaagagaa acaagaagaa ctagaacgcg
      421 tcttaagcaa tgaacgattt cgagggcgtc aaagagtatt aagagactta cgcattgtgt
      481 tt
//
LOCUS       DAVBCR010000168          463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBCR010000168 DAVBCR010000000
VERSION     DAVBCR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.168"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(137..>463)
                     /locus_tag="VQ508_002082"
     CDS             complement(137..>463)
                     /locus_tag="VQ508_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2491005.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
BASE COUNT           90 a           98 c           77 g          198 t
ORIGIN      
        1 tactattcta gtttcaatct actatatttt gcacattttc atgaaaaaaa tgagaactag
       61 aactcacatt ctgctctcat ttttcgtttt cccgttctcc tatcctgttt ttaggagtta
      121 gaaaatgctg ctacctttac ttactctcct ttaataaagc caatagtttt tcagcttctg
      181 ccataatagt attgttgtcc tgggtgccaa atagtaaatt attttttaat cctgtgagag
      241 tctctttggc attggacttg ataattggat cctggatttt tccaagtaaa tcttcagcct
      301 ctctcatttt ttcttcaacc ttttcagtct cgacctgagg ttcttctgat tcctctggtg
      361 attcttcttc tggttcctct gttggttttg gagactctgg tttctcgctt tgcggtttct
      421 cttctcgagg ggtttcttcc tcaggttttt ctgtctgagg ttt
//
LOCUS       DAVBCR010000169          435 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBCR010000169 DAVBCR010000000
VERSION     DAVBCR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.169"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..71
                     /locus_tag="VQ508_002083"
     rRNA            <1..71
                     /locus_tag="VQ508_002083"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            123..195
                     /locus_tag="VQ508_002084"
     tRNA            123..195
                     /locus_tag="VQ508_002084"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Ala,seq:tgc)
     gene            320..>435
                     /locus_tag="VQ508_002085"
     rRNA            320..>435
                     /locus_tag="VQ508_002085"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a           77 c          127 g          105 t
ORIGIN      
        1 tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga
       61 tcacctcctt tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt
      121 gtggggcctt agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga
      181 tcccgctagg ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct
      241 atatcaaata gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga
      301 ctgaaaggtc agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta
      361 ggagccgacg aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg
      421 atccagggat ttccg
//
LOCUS       DAVBCR010000170          409 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVBCR010000170 DAVBCR010000000
VERSION     DAVBCR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.170"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>409
                     /locus_tag="VQ508_002086"
     CDS             <1..>409
                     /locus_tag="VQ508_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2491006.1"
                     /translation="GKKKRHTLKTQIMLDLTTHKVCQMAFSDGHTHDFTLFKESIGQS
                     LPETTLAFVDLGYLGILKFHENTFIPAKNSKNRRLSEDDKQLNKEMSAIRIEIEHFNA
                     KFKTFQIMSVPYRNRRKRFELRAELICAIINYEV"
BASE COUNT          138 a           78 c           76 g          117 t
ORIGIN      
        1 ggtaaaaaga aacgacacac cttaaaaact caaattatgc tggatttgac gacacataaa
       61 gtctgtcaaa tggccttttc tgacggacat acgcatgatt ttactctctt caaagaaagt
      121 attggacaaa gtttgcctga aacgacgctt gcctttgttg acctaggtta tttaggcatc
      181 ttgaaatttc atgagaatac tttcattcct gctaaaaatt ccaaaaatcg ccgcctgagt
      241 gaggatgata agcagttaaa taaagagatg tcagcgatac gaattgaaat tgaacatttt
      301 aacgctaaat tcaagacctt ccaaatcatg tcagtccctt atcgtaaccg cagaaaacgt
      361 ttcgagttac gggcggaatt aatttgtgcc atcatcaatt atgaagtga
//
LOCUS       DAVBCR010000171          372 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVBCR010000171 DAVBCR010000000
VERSION     DAVBCR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.171"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            21..>372
                     /locus_tag="VQ508_002087"
     CDS             21..>372
                     /locus_tag="VQ508_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2491007.1"
                     /translation="METVYDKAQKLNSKNFKLLIGVKKETFQLMLEHLNSAYQIQHRK
                     GGRPRSLPMEDQLIMTLRYLRYYPTQRLLAFDFGVGVATVNAIITWVEDTLRASGSFD
                     LDHLEAPSAAVAIDV"
BASE COUNT          106 a           84 c           80 g          102 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt ga
//
LOCUS       DAVBCR010000172          361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVBCR010000172 DAVBCR010000000
VERSION     DAVBCR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.172"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(46..315)
                     /locus_tag="VQ508_002088"
     CDS             complement(46..315)
                     /locus_tag="VQ508_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2491008.1"
                     /translation="MGLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADET
                     SYRVLESDSQLTYYWTFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT          111 a           70 c           71 g          109 t
ORIGIN      
        1 cgtttggaag acattctaga agataggata gatatttctc actatttata atggattgaa
       61 ataagatatg aacaaatcga ttaggacatg atggtaaagc gtaatccctt gtttctcagc
      121 tttcccagac aaaaaagtcc aatagtaagt cagctgacta tcactctcta gcaccctata
      181 agaagtttca tccgcatgaa gtaagggctg agtcaatagt ctctctcgca agaggttata
      241 aaggggctcc aaatagtatt gactcgtctt gatatgccga ttagagattt ccttacgtgt
      301 gattggtaaa cccatcctag cccaatcttc ttcttggcga taattgggta ccttcagatt
      361 a
//
LOCUS       DAVBCR010000173          359 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVBCR010000173 DAVBCR010000000
VERSION     DAVBCR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.173"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            1..359
                     /locus_tag="VQ508_002089"
                     /pseudo
     CDS             1..359
                     /locus_tag="VQ508_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          112 a           73 c           78 g           96 t
ORIGIN      
        1 catttgaagg ctgcagaggc aggagaattt gttacaattg atgccttatt tcaggcttat
       61 aaaaaggagt taggtcgttc ctacacacgt gatgccttct atcaactgtt gaagcgccat
      121 ggttggcgaa atattacgcc acgtccagaa catcctaaga aagcagacgc tcaaaccatt
      181 gtcgcgtcta aaaataaaat ctcaattcaa gaagacaaga aagcgcttta aaaccagtag
      241 accttttcat aaggttcgct tgatgtacca agatgaggct ggtttcggta gaatcagtaa
      301 actgggatct tgttggtctc caataggagt aggtccacat gtccatagtc actatatac
//
LOCUS       DAVBCR010000174          324 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVBCR010000174 DAVBCR010000000
VERSION     DAVBCR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.174"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>324
                     /locus_tag="VQ508_002090"
     CDS             <1..>324
                     /locus_tag="VQ508_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2491009.1"
                     /translation="IINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFS
                     RLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVK
                     IITMDM"
BASE COUNT           99 a           64 c           57 g          104 t
ORIGIN      
        1 atcatcaacc aaaaaattgc tcaaaagtta attgaaaaaa tttctatgac tgatattgcc
       61 catcagctgg ccatttcaac ttcaactgtc attcgcaagc tcaatgactt tcactttgag
      121 catgattttt ctcggcttcc aaagattatg tcttgggatg agtatgcctt cactaaggga
      181 aagatgagtt tcattgcgca agattttgat aatcttaata ttatcactgt tcttgaaggc
      241 agaacacaag ctgtcatccg aaatcacttt cttcgctacg atagagccgt tcgttgtcaa
      301 gtgaaaatca ttacgatgga tatg
//
LOCUS       DAVBCR010000175          316 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVBCR010000175 DAVBCR010000000
VERSION     DAVBCR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.175"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..175)
                     /locus_tag="VQ508_002091"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VQ508_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           95 a           52 c           54 g          115 t
ORIGIN      
        1 ttgacaatct tctctttatc tttgaggaag gttttagagg atgaacctga ttcagattgt
       61 cctcaatgag tccgaaaaat ttgtcaggct ccttattctg aaagtgaaaa agcaagagtt
      121 gatagagatt atagtggtgt ttcaagtctt ctgaatagct caaaagttta tctatagtag
      181 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt
      241 cctgaatgat ttgtcctgtt attatttcat tttactataa atccacgttt acgaatctct
      301 ttccacactt gttcaa
//
LOCUS       DAVBCR010000176          305 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVBCR010000176 DAVBCR010000000
VERSION     DAVBCR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.176"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..>305)
                     /locus_tag="VQ508_002092"
     CDS             complement(<1..>305)
                     /locus_tag="VQ508_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2491010.1"
                     /translation="SHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKI
                     LSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEK"
BASE COUNT           91 a           44 c           64 g          106 t
ORIGIN      
        1 attttctctt tgttctttag aaaggtttta aagacagtct gaaaaagagg atgaacctgc
       61 ttcagattgt cctcaatgag tccgaaaaat ttctcagggt ctttgttctg aaagtgaaaa
      121 agtaagagtt gatagatctg atagtggtgt ttcaagtctt ctgaatagct taaaatcttg
      181 tcaagaattt ctttatttgt taagtgcatg cgaaaagtag ggcgataaaa acgtttatcg
      241 ctcaatttac gactatcctg ttggatgagt ttccagtaac gcttgatagc cttgtattca
      301 tgaga
//
LOCUS       DAVBCR010000177          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVBCR010000177 DAVBCR010000000
VERSION     DAVBCR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.177"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ508_002093"
     rRNA            51..>292
                     /locus_tag="VQ508_002093"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCR010000178          288 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVBCR010000178 DAVBCR010000000
VERSION     DAVBCR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.178"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQ508_002094"
     CDS             <1..>288
                     /locus_tag="VQ508_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2491011.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVBCR010000179          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVBCR010000179 DAVBCR010000000
VERSION     DAVBCR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.179"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ508_002095"
     CDS             <1..>277
                     /locus_tag="VQ508_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2491012.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCR010000180          272 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVBCR010000180 DAVBCR010000000
VERSION     DAVBCR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.180"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..272)
                     /locus_tag="VQ508_002096"
                     /pseudo
     CDS             complement(1..272)
                     /locus_tag="VQ508_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           75 a           72 c           48 g           77 t
ORIGIN      
        1 atacttgagg ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg
       61 ccggcaccaa gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc
      121 gttcatcagc tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag
      181 ctaaaccttt agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact
      241 tccttctcac atgcgcccaa catccaacaa gt
//
LOCUS       DAVBCR010000181          270 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVBCR010000181 DAVBCR010000000
VERSION     DAVBCR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.181"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>270
                     /locus_tag="VQ508_002097"
     rRNA            <1..>270
                     /locus_tag="VQ508_002097"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           74 c           72 g           55 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca
//
LOCUS       DAVBCR010000182          267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVBCR010000182 DAVBCR010000000
VERSION     DAVBCR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.182"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(1..267)
                     /locus_tag="VQ508_002098"
                     /pseudo
     CDS             complement(1..267)
                     /locus_tag="VQ508_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           85 a           42 c           56 g           84 t
ORIGIN      
        1 tgaaaaagca agagctgata gagttgatag tggtgtttca agtcttccga ataactcaaa
       61 agcttgttta aaatctcttt attggttcaa tgcatacgaa aagtaggacg ataaaaatgt
      121 ttatcactca gtttacgact atcctgttga atgagtttcc agtagcgctt gatagccttg
      181 tattcatggg attttcgatg aaactgattt atgatttgaa cacgcacacg actcatagca
      241 cggctaagat gttgtacaat gtgaaag
//
LOCUS       DAVBCR010000183          261 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVBCR010000183 DAVBCR010000000
VERSION     DAVBCR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.183"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
BASE COUNT           83 a           45 c           51 g           82 t
ORIGIN      
        1 atagagctcg ctcttactat tatagcggat gttatagctg gaattatctt gtatttcgtc
       61 tgcagatggc tagatagtaa ggagtagacc gagtgactag cctataaaca cccgttaaat
      121 cgctaagata cgtcaaaaaa gcccttaact acggccatag ttaggggctt tggtgttcta
      181 atgaacctta tcaattaact acattttagc atataatcta caatagttca agcattaagt
      241 gaggttgatg atctaaattt a
//
LOCUS       DAVBCR010000184          233 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVBCR010000184 DAVBCR010000000
VERSION     DAVBCR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.184"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            complement(<1..>233)
                     /locus_tag="VQ508_002099"
     CDS             complement(<1..>233)
                     /locus_tag="VQ508_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2491013.1"
                     /translation="LLFLKPLYNLLRERLLTQPLLHADETSYRVLESHSHLTYYWTFL
                     SGKVENQAITLYHHDQSRSGSVVQEFLGDYSGYV"
BASE COUNT           71 a           50 c           52 g           60 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaatag gag
//
LOCUS       DAVBCR010000185          229 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVBCR010000185 DAVBCR010000000
VERSION     DAVBCR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.185"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            <1..>229
                     /locus_tag="VQ508_002100"
     CDS             <1..>229
                     /locus_tag="VQ508_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2491014.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH"
BASE COUNT           72 a           49 c           55 g           53 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatc
//
LOCUS       DAVBCR010000186          227 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVBCR010000186 DAVBCR010000000
VERSION     DAVBCR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.186"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
BASE COUNT           71 a           43 c           32 g           81 t
ORIGIN      
        1 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat ttgagtctag
       61 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
      121 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgagcagt ctgcagctaa
      181 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagaca
//
LOCUS       DAVBCR010000187          204 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GA02211
            SAMEA1919809-rid20172923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVBCR010000187 DAVBCR010000000
VERSION     DAVBCR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919809
            Sequence Read Archive: ERR298094
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980259.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980259.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,101
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,944
            CDSs (with protein)               :: 1,944
            Genes (RNA)                       :: 38
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 62 of 119
            Pseudo Genes (incomplete)         :: 52 of 119
            Pseudo Genes (internal stop)      :: 34 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919809-rid20172923.denovo.187"
                     /strain="GA02211"
                     /db_xref="taxon:1313"
     gene            79..152
                     /locus_tag="VQ508_002101"
     tRNA            79..152
                     /locus_tag="VQ508_002101"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Met,seq:cat)
BASE COUNT           47 a           48 c           59 g           50 t
ORIGIN      
        1 gggaacggtc ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc
       61 ctccttttat attattaacg cgggatggag cagctcggta gctcgtcggg ctcataaccc
      121 gaaggtcgta ggttcaaatc ctgctcccgc aataaggctc ggtagctcag ttggtagagc
      181 aatggattga agctccatgt gtcg
//
